NC1194: Nanotechnology and Biosensors

(Multistate Research Project)

Status: Active

Project Menu

SAES-422 Reports

Annual/Termination Reports:

[10/14/2022] [10/05/2023] [10/21/2024]

Date of Annual Report: 10/14/2022

Report Information

Annual Meeting Dates: 08/11/2022 - 08/12/2022
Period the Report Covers: 10/01/2021 - 09/30/2022

Participants

Yanbin Li (Arkansas), Sathish Kumar Ponniah (Arkansas), Jeong-Yeol Yoon (Arizona), Yangchao Luo (Connecticut), Margaret Frey (Cornell), Bin Gao (Florida), Jose I Reyes-De-Corcuera (Georgia), Daniel Jenkins (Hawaii), Yi-Cheng Wang (Illinois), Kurt Ristroph (Indiana), Carmen Gomes (Iowa), Chenxu Yu (Iowa), Olga Tsyusko (Kentucky), Todd Monroe (Louisiana), Naveen Kumar Dixit (Maryland), Joel Pedersen (Maryland), Evangelyn Alocilja (Michigan), Mrinal Bhattacharya (Minnesota), Mengshi Lin (Missouri), Paul Takhistov (New Jersey), Tzuen-Rong Jeremy Tzeng (South Carolina), Diana Vanegas (South Carolina), Eric S McLamore (South Carolina), Zhengrong Gu (South Dakota), Anhong Zhou (Utah),Chenming Zhang (Virginia), Juhong Chen (Virginia), Mike Zhang (Virginia), Sundaram Gunasekaran (Wisconsin)

Brief Summary of Minutes

Station reports were delivered from each participant describing completed and on-going research.

Administrative updates by Hongda Chen and Steve Lommel where Hongda Chen shared information on the collection of the publications on Smart Agriculture in the issue of The Bridge, Summer 2022. He also provided update on the AFRI funding in 2021/2022 with the cap up to $ 650 K and with additional $150 K available for MSIs, small institutions and EPSCoR, he emphasized that the success rate was at 30% for the new investigator seed grants with a $300 K budget and that there is also a postdoc grant for an independent research available to the students within 6-8 months of their graduation.

Steve Lommel reminded that the annual report is due within 60 days and that the report should follow abbreviated format (3-page limit) with a stronger emphasis on impacts and products and alignment to societal challenges. He pointed out that continued collaboration and productivity are the key for the future success in the multistate project and conveyed that based on our impactful collaborative multistate activities we have an opportunity to be nominated for the APLU award.

Anhong Zhou was elected as Secretary for the 2022-2023 term. Jose Reyes-De-Corcuera will become the chair and Chenxu Yu will become the vice-chair for the 2022-2023 term starting immediately after the meeting. The remainder of the meeting was devoted to discussing options for 2023 meeting, collaborations, joined publications, grant applications, and preparing a conference proposal. Full minutes are available at NIMSS website under Reports.

Lommel, Steve (slommel@ncsu.edu) – North Carolina State University / project administrator; Chen, Hongda (hongda.chen@usda.gov) – USDA-NIFA.

Accomplishments

Contributions to the objectives of this project on its first year are summarized. At least 46 publications associated to the research and listed and the end of this report were published by the NC-1194 group. <ol> <li>Develop new technologies for characterizing fundamental nanoscale processes and fabricate self-assembled nanostructures</li> </ol> Outputs: Surface-enhanced Raman spectroscopy (SERS) coupled with gold nanostars (AuNS) for rapid detection of paraquat residues in green tea was developed. The spiky tips of AuNS can serve as SERS hot spots to intensify Raman signals of analyte molecules and enhance substrate-analyte interactions resulting in a detection limit of this SERS method is 0.2 mg/kg. Cellulose nanofiber wipers fabricated on quartz paper coated with silver nanoparticles and AuNS detected ferbam on kale leaves with a detection limit was 50 µg/kg (MO). Spectroscopic imaging methods for analyzing protein-nanoplastics interactions were developed, Also methods to make functional food with enhanced nutritional values and quality (IA). Differential scanning fluorimetry at high hydrostatic pressure was developed to determine the melting temperature of proteins and better characterize their thermal unfolding mechanism (GA). <ol start="2"> <li>Develop devices and systems incorporating nanotechnology and data-driven analytics for detection of biological/chemical targets, with an emphasis on detection of infectious diseases in plants, animals, humans, and the environment</li> </ol> Outputs: Research on the characterization and understanding of environmental health toxicity and toxicity mechanisms of the various nanomaterials used in agriculture before and after environmental transformations in simple and complex exposure scenarios continued this year. The potential foliar application of plant-activated nitrogen nanocarriers to non-targeted soil and microbial communities was examined. The toxicity and potential applications of the composite membranes with 2D nanomaterials (phosphorene and hexagonal boron nitride) for degradation of the PFAS in drinking water was analyzed. (KY). Methods and nanomaterials to better understand and improve the mechanisms that control mixed manure composting and methods to monitor antibiotic-resistant bacteria during fresh produce production using municipal wastewater effluents were developed (IA). Nano-delivery systems for marine-originated polyphenols as therapeutics were developed (IA), Portable gene-based and immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva were developed (IA, HI). First generation phosphate sensors based on stimulus-response nanobrush electrodes or on carbon nanoparticles, nitrogen sensors based on laser inscribed graphene electrodes, and Listeria spp. sensors were developed (SC IA). A novel InvG protein as a potential biosensor for Salmonella spp is being researched (SC FL). Functionalized iron-oxide nanoparticles for targeted cancer therapy have been developed (SC). A norovirus detection and the capillary flow profile method were tested for human saline samples and aerosol samples for detecting SARS-CoV-2 and a paper microfluidic and smartphone detection for toxic mushrooms and THC from human saliva were tested (AZ). A new affordable optical-based instrument (based on simple modification to an existing instrument) was built to conduct gene-based diagnostic assays, and its application for detecting SARS-CoV-2 virus in human saliva. A fluidics and image processing system to support the development of a related instrument, where samples can be tested for multiple pathogens and internal controls simultaneously with minimal pipetting, on a novel / low cost thermoformed plastic card was demonstrated and new electrode array systems based on customized “flex” circuit manufacture overlaid with low cost laser inscribed graphene networks, supporting disposable diagnostics development were made and interfaced with support instrumentation were made (HI) <ol start="3"> <li>Advance the integration of novel sensor networks, information systems, and artificial intelligence for effective risk assessment and decision support for food security and safety</li> </ol> Outputs: AI-driven imaging techniques for disease diagnosis and graphene potentiometric ion-selective electrodes for nitrate sensing were developed (IA). Machine learning (ML) tools were created to analyze Raman spectra to analyze the overall features of different macrophage subtypes in the study the anti-inflammatory effect of docosahexaenoic acid (DHA) in mouse macrophage cell line. Cytokine analysis and immunofluorescence imaging were also performed to validate and compare with Raman data (UT). A ML suite of for predicting E. coli concentration in alternative water sources used for leafy green irrigation and frost protection as well as a hierarchical clustering algorithm for cloud-based analysis of sensor-to-sensor variation during manufacturing was created in collaboration with the SmartPath Center of Excellence (NIFA Project No. 2018-67016-27578), (SC FL).  A method ML-based classification was tested for identifying oil spills, unknown bacterial species, and human NK cell subpopulations (AZ). <ol start="4"> <li>Develop and update education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment.</li> </ol> Outputs: Short courses on Nanotechnology (NJ, KY), Biosensors (SC) and Entrepreneurship for Scientist (MI) at the international Global Alliance for Rapid Diagnostics (GARD) symposium with participants from 37 countries was taught. Also sessions on Nano-biointeractions and environmental responses at Society of Environmental Toxicology and Chemistry (SETAC) North America meeting and at Nanoweek and Nanocommons conference in Limassol, Cyprus were organized (KY). Serving as US co-chair for US-EU Nanoecotoxicity COR to reach out to researchers from academia and industry working on Nanosafety. (KY). A REU-site on wearable graphene-based stress biosensor development with community college and high school students starting summer 2021 and educational tools for inspiring self-stewardship as well as racial inclusiveness were created (IA). A new 267-page textbook on tissue engineering with laboratory exercises and 126 figures was published (AZ). <ol start="5"> <li>Increase the number academic-industry partnerships to help move the developed technologies to commercialization phase.</li> </ol> IA station worked with a IUCRC team to improve academic-industry partnership and develop technologies for soil dynamics to promote commercialization of the technology

Publications

<ol> <li>Akarapipad A, Kaarj K, Breshears LE, Sosnowski K, Baker B, Nguyen BT, Eades C, Uhrlaub JL, Quick G, Nikolich-Zugich J, Worobey M, and Yoon J-Y, "Smartphone-based Sensitive Detection of SARS-CoV-2 from Saline Gargle Samples via Flow Profile Analysis on Paper Microfluidic Chip," Biosensors and Bioelectronics, 2022, 207: 114192.</li> <li>Angelé-Martínez, C., F.S. Ameer, Y.S. Raval, G. Huang, T.R.J. Tzeng, J.N Anker, J. Brumaghim (2022) Polyphenol effects on CuO-nanoparticle-mediated DNA damage, reactive oxygen species generation, and fibroblast cell death. Toxicology in Vitro 78: 105252.</li> <li>Breshears LE, Nguyen BT, Akarapipad P, Sosnowski K, Kaarj K, Quirk G, Uhrlaub JL, Nikolich-Zugich J, Worobey M, and Yoon J-Y, "Sensitive, Smartphone-based SARS-CoV-2 Detection from Clinical Saline Gargle Samples," PNAS Nexus, 2022, 1(1): pgac028.</li> <li>Breshears LE, Nguyen BT, Mata-Robles S, Wu L, and Yoon J-Y, "Biosensor Detection of Airborne Respiratory Viruses Such As SARS-CoV-2," SLAS Technology, 2022, 27(1): 4-17.</li> <li>Bahng EJ, Rutenberg A, Gomes C. Spectrum and Matrix of Inclusion Tendencies and Experiences using Narrative Stories, Iowa State Conference on Race and Ethnicity, 23(1), 2022</li> <li>Buchanan BC and Yoon J-Y, "Microscopic Imaging Methods for Organ-on-a-Chip Platforms," Micromachines, 2022, 13: 328.</li> <li>Buchanan BC, Safavinia B, Wu L, and Yoon J-Y, "Smartphone-Based Autofluorescence Imaging to Detect Bacterial Species on Laboratory Surfaces," Analyst, 2022, 147: 2980-2987. Analyst HOT Articles 2022.</li> <li>Day AS, Ulep T-H, Budiman E, Dieckhaus L, Safavinia B,  Hertenstein T, Yoon J-Y, "Contamination-resistant, Rapid Emulsion-based Isothermal Nucleic Acid Amplification with Mie-scatter Inspired Light Scatter Analysis for Bacterial Identification," Scientific Reports, 2021, 11: 19933.</li> <li>Diaz, L. M., B. E. Lee, and D. M. Jenkins. 2021. Real-time optical analysis of a colorimetric LAMP assay for SARS-CoV-2 in saliva with a handheld instrument improves accuracy compared to endpoint assessment. Journal of Biomolecular Techniques. 32(3): 158–171. https://doi.org/10.7171/jbt.21-3203-011.</li> <li>Domingo, R., C. Perez, D. Klair, H. Vu, A. Candelario-Tochiki, X. Wang, A. Camson, J. N. Uy, M. Salameh, D. Arizala, S. Dobhal, G. Boluk, J.-P. Bingham, F. Ochoa-Corona, M. E. Ali, J. P. Stack, J. Fletcher, J. Odani, D. M. Jenkins, A. M. Alvarez, and M. Arif, M. 2021. Genome-informed loop-mediated isothermal amplification assay for specific detection of Pectobacterium parmentieri in infected potato tissues and soil. Sci. Rep. 11, 21948. <a href="https://doi.org/10.1038/s41598-021-01196-4">https://doi.org/10.1038/s41598-021-01196-4</a>.</li> <li>Chen* C., Unrine J.M., Hu Y., Guo L., Tsyusko O.V., Fan Z., Liu S., Wei G. 2021. Responses of soil bacteria and fungal communities to pristine and sulfidized zinc oxide nanoparticles relative to Zn ions. Journal of Hazardous Materials 405, 124258.</li> <li>Choo, K. W., Dhital, R., Mao, L., Lin, M., Mustapha, A. 2021. Development of polyvinyl alcohol/chitosan/ modified bacterial nanocellulose films incorporated with 4-hexylresorcinol for food packaging applications. Food Packag. Shelf Life. 30, 100769</li> <li>Cícero C Pola, Sonal V Rangnekar, Robert Sheets, Beata M Szydłowska, Julia R Downing, Kshama W Parate, Shay G Wallace, Daphne Tsai, Mark C Hersam, Carmen L Gomes, Jonathan C Claussen, Aerosol-jet-printed graphene electrochemical immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva, 2D materials, 9(3), 035016, 2022.</li> <li>Diehl, M., Kang, M.J., Reyes-De-Corcuera, J.I.* (2022) Effect of high-pressure technologies on enzymes used in nonfood processing applications in Effect of High Pressure Technologies on Enzymes, Leite Júnior, B. and Tribst, A., Editors. Academic Press. In press</li> <li>Eke J., Banks L., Mottaleb M.A., Morris A.J., Tsyusko O.V., and Escobar* I.C. 2021. Dual-Functional Phosphorene Nanocomposite Membranes for the Treatment of Perfluorinated Water: An Investigation of perfluorooctanoic acid removal via filtration combined with ultraviolet irradiation or oxygenation. Membranes 11, no. 1: 18.</li> <li>Eluchie C, Hu H, Johnson Z, Gomes C, Claussen J, Hu H, An Explorative Study to Use Graphene-based Materials for Aircraft Icing Mitigation, AIAA AVIATION 2022 Forum, 4115, 2022. <a href="https://doi.org/10.2514/6.2022-4115.vid">https://doi.org/10.2514/6.2022-4115.vid</a></li> <li>Hjort R, Gomes CL, The Importance of Sensors in Water Quality Research and Monitoring, Resource Magazine, 29(4), 17-19, 2022</li> <li>Hjort, R.G., R.R.A. Soares, J. Li, D. Jing, L. Hartfiel, B. Chen, B. Van Belle, M. Soupir, E. Smith, E.S. McLamore, J.C. Claussen, C.L. Gomes (2022) Hydrophobic laser-induced graphene potentiometric ion-selective electrodes for nitrate sensing. Microchimica Acta 189 (3), 1-11.</li> <li>Jiao, C., Z. Guo, J. Gong, Y. Zuo, S. Li, D. Vanegas, E.S. McLamore, Y. Shen (2022) CML8 and GAD4 function in (Z)–3–hexenol–mediated defense by regulating γ–aminobutyric acid accumulation in Arabidopsis. Plant Physiology and Biochemistry 186, 135-144.</li> <li>Kim S, Patarajarin Akarapipad, Brandon T. Nguyen, Lane E. Breshears, Katelyn Sosnowski, Jacob Baker, Jennifer L. Uhrlaub, Janko Nikolich-Zugich, and Jeong-Yeol Yoon, "Direct Capture and Smartphone Quantification of Airborne SARS-CoV-2 on a Paper Microfluidic Chip," Biosensors and Bioelectronics, 2022, 200: 113912</li> <li>Kim S, Day AS, and Yoon J-Y, "Machine Learning Classification of Bacterial Species Using Mix-and-Match Reagents on Paper Microfluidic Chips and Smartphone-based Capillary Flow Analysis," Analytical and Bioanalytical Chemistry, 2022, 414: 3895-3904.</li> <li>Kim S, Ciara Eades, and Yoon J-Y, "COVID-19 Variants’ Cross-Reactivity on the Paper Microfluidic Particle Counting Immunoassay," Analytical and Bioanalytical Chemistry, 2022, doi:10.1007/s00216-022-04333-8. Papers in Forefront.</li> <li>Lee, B.-E., T. Kang,  M. Jenkins, Y. Li, M. Wall, and S. Jun. 2022. A single-walled carbon nanotubes-based electrochemical impedance immunosensor for on-site detection of Listeria monocytogenes. Journal of Food Science. 87(1): 280-288. 10.1111/1750-3841.15996</li> <li>Liang Y, Avory Zhou, Candace S. Bever, Luisa W. Cheng, and Jeong-Yeol Yoon, "Smartphone-Based Paper Microfluidic Competitive Immunoassay for the Detection of Alpha-Amanitin from Mushrooms," Microchimica Acta, 2022, 189: 322.</li> <li>Liang Y, Avory Zhou, and Jeong-Yeol Yoon, "Machine Learning-Based Quantification of (-)-trans-delta-Tetrahydrocannabinol from Human Saliva Samples on a Smartphone-Based Paper Microfluidic Platform," ACS Omega, 2022, 7(34): 30064–30073.</li> <li>Lin, M.-H., Sun, L., Kong, F., Lin, M. 2021. Rapid detection of paraquat residues in green tea using surface-enhanced Raman spectroscopy (SERS) coupled with gold nanostars. Food Control. 130, 108280.</li> <li>Mannier C and Yoon J-Y, "Progression of LAMP as a Result of the COVID-19 Pandemic: Is PCR Finally Rivaled?" Biosensors, 2022, 12: 492.</li> <li>McLamore, E.S., G. Moreira, D.C. Vanegas, S.P.A. Datta (2022) Context-Aware Diagnostic Specificity (CADS). Biosensors 12 (2), 101.</li> <li>Oliveira, D.A., S. Althawab, E.S. McLamore, C.L. Gomes (2021) One-step fabrication of stimuli-responsive chitosan-platinum brushes for Listeria monocytogenes detection. Biosensors, 1(12), 511; DOI: 10.3390/bios11120511.</li> <li>Reyes-De-Corcuera, J.I.* (2022) Enzymes – Inspring evolutionary wisdom. In nanozymes, advances and applications. Gunasekaran, S., Editor, CRC Press. 1-14.</li> <li>Sosnowski K, Loh A, Zubler AV, Shir H, Ha SY, Yim UH, Yoon J-Y, "Machine Learning Techniques for Chemical and Type Analysis of Ocean Oil Samples via Handheld Spectrophotometer Device," Biosensors and Bioelectronics: X, 2022, 10: 100128.</li> <li>Summerlin, H., C.C Pola, K.R. Chamakura K. Borel, R. Young, T. Gentry, E.S. McLamore, R. Karthikeyan, C. Gomes, (2021). Fate of enteric viruses during leafy greens (romaine lettuce) production using treated municipal wastewater and AP205 bacteriophage as a surrogate. J. Environmental Science and Health, Part A. 56(10): 1138-1144.</li> <li>Sun J, Liu X, Wang Z, Yin F, Liu H, Nakamura Y, Yu C, Zhou D, Gastrointestinal digestion and absorption characterization in vitro of zinc‐chelating hydrolysate from scallop adductor (Patinopecten yessoensis), Journal of the Science of Food and Agriculture, 102(8), 3277-3286, 2022. https://doi.org/10.1002/jsfa.11673.</li> <li>Wang Z, Sun J, Ma X, Liu X, Yin F, Li D, Nakamura Y, Yu C, Zhou D, Characterization of a synthetic zinc‐chelating peptide from sea cucumber (Stichopus japonicus) and its gastrointestinal digestion and absorption in vitro, Journal of the Science of Food and Agriculture, 2022, https://doi.org/10.1002/jsfa.11811</li> <li>Xie Y, Yu X, Wang Y, Yu C, Prakash S, Zhu B, Dong X, Role of dietary fiber and flaxseed oil in altering the physicochemical properties and 3D printability of cod protein composite gel, Journal of Food Engineering, 327, 111053, 2022 https://doi.org/10.1016/j.jfoodeng.2022.111053</li> <li>Xie Y, Yu X, Wang Z, Yu C, Prakash S, Dong X, The synergistic effects of myofibrillar protein enrichment and homogenization on the quality of cod protein gel. Food Hydrocolloids, 127, 107468, 2022 https://doi.org/10.1016/j.biortech.2021.126626</li> <li>Yoon J-Y, "Tissue Engineering: A Primer with Laboratory Demonstrations," Springer: Cham, Switzerland, 2022, ISBN: 978-3-030-83695-5.</li> <li>Yin S, Zhang W, Tong T, Yu C, Chang X, Chen K, Xing Y, Yang Y, Feedstock-dependent abundance of functional genes related to nitrogen transformation controlled nitrogen loss in composting, Bioresource Technology, 2022, https://doi.org/10.1016/j.biortech.2021.126626</li> <li>Yu C, Takhistov P, Alocilja E, Reyes de Corcuera J, Frey MW, Gomes CL, Mao Y, McLamore ES, Lin M, Tsyusko OV, Tzeng T-R, Yoon J-Y, Zhou A, Bioanalytical approaches for the detection, characterization, and risk assessment of micro- and nano-plastics in agriculture and food systems, Analytical and Bioanalytical Chemistry, 2022. https://doi.org/10.1007/s00216-022-04069-5.</li> <li>Yu X, Wang Y, Xie Y, Wei S, Ding H, Yu C, Dong X, Gelation properties and protein conformation of Grass Carp fish ball as influenced by egg white protein, Journal of Texture Studies. 2022. https://doi.org/10.1111/jtxs.12668</li> <li>Yu W, Wang Z, Pan Y, Jiang P, Pan J, Yu C, Dong X, Effect of κ-carrageenan on quality improvement of 3D printed Hypophthalmichthys molitrix-sea cucumber compound surimi product, LWT, 154, 112279, 2022. <a href="https://doi.org/10.1016/j.lwt.2021.112279">https://doi.org/10.1016/j.lwt.2021.112279</a></li> <li>Zachary T Johnson, Nathan Jared, John K Peterson, Jingzhe Li, Emily A Smith, Scott A Walper, Shelby L Hooe, Joyce C Breger, Igor L Medintz, Carmen Gomes, Jonathan C Claussen, Enzymatic Laser‐Induced Graphene Biosensor for Electrochemical Sensing of the Herbicide Glyphosate, Global Challenges, 6(9), 2200057, 2022 <a href="https://doi.org/10.1002/gch2.202200057">https://doi.org/10.1002/gch2.202200057</a>.</li> <li>Zenhausern R, Alexander S. Day, Babak Safavinia, Seungmin Han, Paige E. Rudy, Young-Wook Won, and Jeong-Yeol Yoon, "Natural Killer Cell Detection, Quantification, and Subpopulation Identification on Paper Microfluidic Cell Chromatography Using Smartphone-based Machine Learning Classification," Biosensors and Bioelectronics, 2022, 200: 113916.</li> <li>Zhang W., Karagiannidis I., Van Vliet E.D.S, Yao R., Beswick E., Zhou A., "Effects of granulocyte colony-stimulating factor on colon and breast cancer cells investigated by machine learning based non-invasive Raman spectroscopy", Analyst. 2021, 146, 6124-6131</li> <li>Zhang W, Yu C, Wang X, Yin S, Chang X, Additives improved saprotrophic fungi for formation of humic acids in chicken manure and corn stover mix composting, Bioresource Technology, 346, 126626, 2022, https://doi.org/10.1016/j.biortech.2021.126626</li> <li>Zhao, Y., Zhang, W., Devener, B.V., Bunch, T.D., Zhou, A., Isom, S. Clay, “In situ characterization of porcine fibroblasts in response to silver ions by Raman spectroscopy and liquid scanning transmission electron microscopy”, Talanta, 2022, 246 (15) 123522.</li> </ol>

Impact Statements

The research carried out by the group increased the understanding of nanotechnology and biosensors by the gen-eral public by bringing greater awareness of their current and potential roles in food and agriculture. At least 5 post-docs, 21 PhD students, 8 MS students, and 21 undergraduate students participated in this project. 1. Research carried out this year resulted an easy and cost-effective way to fabricate high-performance substrates for SERS. The results indicate that SERS coupled with gold nanostars is a practical approach and has great po-tential to be applied for the qualification and quantification of trace contaminants in foods (IA). 2. Data of the safety and toxicity of the commonly used nanomaterials can inform policy decisions related to manufacturing, use, and exposure to these materials to safeguard public and environmental health. Transfor-mational change in the way nitrogen is used is required and sustainable nitrogen fertilization in crops using safe N nanocarriers has a potential to decrease soil nitrate leaching and greenhouse gas emissions (KY). 3. A proposed non-invasive Raman spectroscopy and specific antibody conjugated SERS probes offer new tools that can be used to develop new drugs against pro-inflammatory macrophages induced chronic diseases, such as inflammatory bowel disease (IBD) and obesity (UT). 4. New “single tube” systems integrated with ABE-STAT for testing irrigation water, drinking water samples and for optical biosensors in the field that use a common smartphone that will be coupled to a decision support app were developed and applied to SARS-Co-2 and to apple stem pitting virus (SC HI NSF STEPS). 5. A smartphone-based autofluorescence detection of bacterial contamination was featured as Analyst HOT Arti-cles 2022. 6. A novel method and user-friendly device have been proposed and successfully demonstrated using of a smartphone-based fluorescence microscope to paper microfluidic devices was demonstrate for SARS-CoV-2 and cancer cells, toxins and THC from human saliva. Novel machine learning algorithms were implemented to our handheld biosensors and paper microfluidic chips, for classifying oil spills, unknown bacterial species, and la-boratory bacterial contamination. These devices can be used by a lay person in myriads of environmental con-ditions. Such demonstrations will protect the general public from potential health risks from food, water, aero-sols, and humans (AZ). 7. A correlation between protein cavities and the optimal pressure for the stability of an enzyme was developed this will allow predicting the optimal conditions for enzyme catalysis at high pressure (GA) 8. The design of an existing open-source, palm-sized instrument for electrochemical measurements to improve performance for a wide variety of molecular diagnostics, with the aim of advancing the commercialization of new electrochemical based biosensor technologies being developed by collaborators at multiple participating states (HI). Outcomes. Received collaborative grants from NSF ECO-CBET “Foliar applied plant-activated nitrogen delivery agents for sustainable crop production” and NSF grant on Transformation, interaction and toxicity of emerging 2D nano-materials free-standing and embedded onto nanocomposite membranes for PFAS degradation.

Date of Annual Report: 10/05/2023

Report Information

Annual Meeting Dates: 08/07/2023 - 08/08/2023
Period the Report Covers: 10/01/2022 - 09/30/2023

Participants

Yanbin Li (Arkansas), Jose I Reyes-De-Corcuera (Florida), Daniel Jenkins (Hawaii), Chenxu Yu (Iowa), Olga Tsyusko and Jarad Cochran (Kentucky), Evangelyn Alocilja (Michigan), Mengshi Lin (Missouri), Paul Takhistov (New Jersey), Eric S McLamore (South Carolina), Zhengrong Gu (South Dakota), Anhong Zhou (Utah), Chenming Zhang (Virginia)

Brief Summary of Minutes

Station reports were delivered from each participant describing completed and on-going research.

Administrative updates by Hongda Chen and Steve Lommel where Hongda Chen shared information on the upcoming AFRI funding in 2022/2023 with the cap up to $ 650 K and with additional $150 K available for MSIs, small institutions and EPSCoR, he emphasized that the success rate was at 30% for the new investigator seed grants with a $300 K budget and that there is also a postdoc grant for an independent research available to the students within 6-8 months of their graduation. He encouraged continuation of collaborative committee activities to generate impacts both domestically and internationally, and document the impacts to support the mission of NIFA and multistate hatch programs.

Steve Lommel reminded that the annual report is due within 60 days and that the report should follow abbreviated format (3-page limit) with a stronger emphasis on impacts and products and alignment to societal challenges. He pointed out that continued collaboration and productivity are the key for the future success in the multistate project and conveyed that with better documentation and evidence presentation we could again pursue an opportunity to be nominated for the APLU award.

Zhengrong Gu was elected as Secretary for the 2023-2024 term. Chenxu Yu will become the chair and Anhong Zhou will become the vice-chair for the 2023-2024 term starting immediately after the meeting. The remainder of the meeting was devoted to discussing options for 2024 meeting, collaborations, joined publications, grant applications, and exploring collaboration with another multistate project S1077 “enhancing microbial food safety by risk analysis”. and rewrite a conference proposal co-sponsored by NC1194 and S1077 for NIFA. Full minutes are available at NIMSS website under Reports.

Accomplishments

Contributions to the objectives of this project on its first year are summarized. At least 46 publications associated to the research and listed and the end of this report were published by the NC-1194 group. <ol> <li>Develop new technologies for characterizing fundamental nanoscale processes and fabricate self-assembled nanostructures</li> </ol> Outputs: A novel SERS substrate by electrospinning for the detection of thiabendazole in soy-based foods was developed. The approach combines electrospun substrates and SERS for enhanced sensitivity and selectivity. Spectroscopic imaging methods for analyzing protein-nanoplastics interactions were developed, The results demonstrated a linear relationship between intensity and concentration within the range of 100 to 1000 ppb for the soy sauce sample and 10 to 600 ppb for the soymilk sample, with high coefficient of determination values (99.75% and 99.42%, respectively). The limits of quantification (LOQ) were determined to be 69.9 and 240.59 ppb for soymilk and soy sauce samples, respectively, indicating the sensitivity of the method, while the limits of detection (LOD) were 23.1 ppb for soymilk and 79.4 ppb for soy sauce (MO). Methods to make nanocarriers for drug/vaccine delivery and functional food with enhanced nutritional values and quality were developed (IA). Novel adjuvants that can be incorporated into nanoparticles in vaccine was developed (VT); Differential scanning fluorimetry at high hydrostatic pressure was developed to determine the melting temperature of proteins and better characterize their thermal unfolding mechanism (FL). Electrochemical analyses, including impedance and differential pulse voltammetry, were developed for biomolecule interactions such as DNA chain hybridization in nano-scale, small molecule-hydrogel of biopolymers, and small molecule-2D metal organic films (SD). <ol start="2"> <li>Develop devices and systems incorporating nanotechnology and data-driven analytics for detection of biological/chemical targets, with an emphasis on detection of infectious diseases in plants, animals, humans, and the environment</li> </ol> Outputs: Research on the characterization and understanding of environmental health toxicity and toxicity mechanisms of the various nanomaterials used in agriculture before and after environmental transformations in simple and complex exposure scenarios, such as plant-activated nitrogen nanocarriers to non-targeted soil and microbial communities was conducted, the toxicity and potential applications of the composite membranes with 2D nanomaterials (phosphorene and hexagonal boron nitride) for degradation of the PFAS in drinking water was analyzed. (KY). Methods to better understand and improve the mechanisms of spreading of antibiotic-resistant genes during manure composting were developed (IA). Portable gene-based and immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva were developed (IA, HI). First generation phosphate sensors based on stimulus-response nanobrush electrodes or on carbon nanoparticles, nitrogen sensors based on laser inscribed graphene electrodes, and Listeria spp. as well as Salmonella sensors were developed (SC IA). A novel InvG protein as a potential biosensor for Salmonella spp is being researched (SC FL). The team in MSU developed and validated the SMART biosensor to rapidly extract and detect foodborne pathogens, such as Salmonella enterica, E. coli O157:H7, Staphylococcus aureus, and Bacillus cereus from large-volume complex food and farm samples, such as fresh produce (spinach, broccoli, lettuce), meat (beef, chicken), environmental samples (water), and clinical samples (human isolates). They also developed the African Swine Fever Biosensor to detect ASF in farm settings rapidly. The ASF biosensor is being validated in ASF-infected farm samples by our international collaborators (MI). The Utah State team investigated cellular oxidative stress in response to polystyrene (PS) nanoparticles (PS50 for 50 nm, PS500 for 500 nm, and PS100-NH2 for 100 nm with amino group (UT). Design improvements were made to an open-source potentiostat project (intended to accelerate commercialization of electrochemical based biosensors) to improve performance, and importantly to facilitate multiplexed detection of multiple samples and / or biological targets simultaneously through adaptable switched networks. We also successfully demonstrated a new low-cost microfluidic actuation to enable multiplexed detection of optical based biosensors (i.e. for gene-based diagnostics), for implementation with high quality field tests with built in internal controls. (HI) <ol start="3"> <li>Advance the integration of novel sensor networks, information systems, and artificial intelligence for effective risk assessment and decision support for food security and safety</li> </ol> Outputs: AI-driven spectroscopic imaging techniques for CWD diagnosis was developed (IA). Machine learning (ML) tools were created to analyze Raman spectra to analyze the stresses in cells induced by exposure of nanoplastics (UT). Networks of custom surveillance systems were implemented to monitor traps for invasive beetles, with potential to guide operations to eliminate breeding sites, and identify new invasions at ports of entry (HI). <ol start="4"> <li>Develop and update education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment.</li> </ol> Outputs: The Michigan state team held the 2023 GARD Forum virtually on March 20-26, 2023, which was attended by more than 700 participants from more than 30 countries in six continents. Technical sessions, short courses, and an Innovation Challenge were held during the forum. The team also demonstrated the technology developed by them at the MSU Science Festival in April 2023, and trained two minority students under the Summer Research Opportunity Program (SROP). Short courses on Nanotechnology were taught (NJ, KY), course on biosensors was developed and taught (FL). A REU-site on wearable graphene-based stress biosensor development with community college and high school students starting summer 2021 continued through 2022-2034, and educational tools for inspiring self-stewardship as well as racial inclusiveness were created (IA). <ol start="5"> <li>Increase the number academic-industry partnerships to help move the developed technologies to commercialization phase.</li> </ol> IA station continued working with a IUCRC team to improve academic-industry partnership and develop technologies for soil dynamics to promote commercialization of the technology.

Publications

<ol> <li>Caliskan-Aydogan O1, Sharief S1, and Alocilja EC3. 2023. Rapid Isolation of Low-level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles, Biosensors 2023, 13(10), 902; https://doi.org/10.3390/bios13100902</li> <li>Caliskan-Aydogan O1 and Alocilja EC3. 2023. A Review of Carbapenem Resistance in Enterobacterales and Its Detection Techniques. Microorganisms, 2023, 11(6), 1491; https://doi.org/10.3390/microorganisms11061491</li> <li>Sharief, S. A., Caliskan-Aydogan, O., & Alocilja, E. C. 2023. Carbohydrate-coated nanoparticles for PCR-less genomic detection of Salmonella from fresh produce. Food Control, 150 (2023) 109770</li> <li>Boodoo C1, Dester E1, David J1, Patel V1, Rabin KC, and Alocilja EC3. 2023. Multi-Probe Nano-Genomic Biosensor to Detect S. aureus from Magnetically-Extracted Food Samples, Biosensors, accepted, in press. Biosensors 2023, 13(6), 608</li> <li>Sharief S1, Caliskan-Aydogan O1, Alocilja EC3. 2023. Carbohydrate-coated nanoparticles for PCR-less genomic detection of Salmonella from fresh produce, Food Control, Vol. 150, 109770.</li> <li>Boodoo C1, Dester E1, Sharief S1, and Alocilja EC3. 2023. Influence of Biological and Environmental Factors in the Extraction and Concentration of Foodborne Pathogens using Glycan-Coated Magnetic Nanoparticles,  Journal of Food Protection, 86(4):100066.</li> <li>Sharief SA1, Caliskan-Aydogan O1, and Alocilja EC3. 2023. Carbohydrate-coated nanoparticles for point-of-use food contamination testing, Biosensors and Bioelectronics: X, 13 (2023), 100322, 9 pp.</li> <li>Bhattarai RK1,3, Basnet HB, Dhakal IP, Alocilja E. 2023. Virulence genes of avian pathogenic Escherichia coli isolated from commercial chicken in Nepal, Comparative Immunology, Microbiology and Infectious Diseases, 95 (2023) 101961,</li> <li>Caliskan-Aydogan O1, Sharief S1, and Alocilja EC3. 2023. Nanoparticle-Based Plasmonic Biosensor for the Unamplified Genomic Detection of Carbapenem-Resistant Bacteria, Diagnostics, Diagnostics 2023, 13(4), 656</li> <li>Yuanzhi Bian, Debra L. Walter, Chenming Zhang. Efficiency of interferon-γ in activating dendritic cells and its potential synergy with toll-like receptor agonists. Viruses (MDPI). 2023, 15, 1198.doi.org/10.3390/v15051198.</li> <li>Hajikhani, M., Zhang, Y., Gao, X., Lin, M. 2023. Advances in CRISPR-based SERS detection of food contaminants: A review. Trends Food Sci. Technol. 138, 615-627.</li> <li>Wang, W., Yu, Z., Lin, M., Mustapha, A. 2023. Toxicity of silver nanoparticle incorporated-bacterial nanocellulose to human cells and intestinal bacteria. Int. J. Biol. Macromol. 241, 124705.</li> <li>Weng, Z., You, Z., Li, H., Wu, G., Song, Y., Sun, H., Fradlin, A., Neal-Harris, C., Lin, M., Gao, X., Zhang, Y. 2023. CRISPR-Cas12a biosensor array for ultrasensitive detection of unamplified DNA with single-nucleotide polymorphic discrimination. ACS Sensors. 8(4), 1489-1499.</li> <li>Asgari, S., Dhital, R., Mustapha, A., Lin, M. 2022. Duplex detection of foodborne pathogens using a SERS optofluidic sensor coupled with immunoassay. Int. J. Food Microbiol. 383, 109947.</li> <li>Hajikhani, M., Lin, M. 2022. A review on designing nanofibers with high porous and rough surface via electrospinning technology for rapid detection of food quality and safety attributes. Trends Food Sci. Technol. 128, 118-128.</li> <li>Asgari, S., Dhital, R., Ali Aghvami, S., Mustapha, A., Zhang, Y., Lin, M. 2022. Separation and detection of E. coli O157:H7 using a SERS-based microfluidic immunosensor. Microchimica Acta. 189, 111.</li> <li>Diehl, M., Kang, M.J., Reyes-De-Corcuera, J.I.* (2023) Effect of high-pressure technologies on enzymes used in nonfood processing applications in Effect of High-Pressure Technologies on Enzymes, Leite Júnior, B. and Tribst, A., Editors. Academic Press, 405-424.</li> <li>Tong T,* Qi Y, Bussiere L, Dhar D, Miller C, Yu C, Wang Q, Rational Design of Oral Vaccines by Gut Organoid Mucosal Models, Bioactive Materials, 2023, 30, 116-128, <a href="https://doi.org/10.1016/j.bioactmat.2023.07.014">https://doi.org/10.1016/j.bioactmat.2023.07.014</a></li> <li>Zhao M, Cao X, Wu Y, Zou S, Li Z, Lin X, Ji C, Dong L, Zhang S, Yu C, Liang H, Effects of prebiotics on the fermentation of traditional suancai of Northeast China, Food Science and Human Wellness, 2023, <a href="https://doi.org/10.26599/FSHW.2022.9250114">https://doi.org/10.26599/FSHW.2022.9250114</a></li> <li>Zhou Y, Zheng J, Zhao J, Li S, Xing J, Ai C, Yu C, Yang S, Yang J, Oxygenated storage alleviates autolysis of the sea cucumber Apostichopus japonicus during transport. Aquaculture International, 2023. <a href="https://doi.org/10.1007/s10499-023-01108-5">https://doi.org/10.1007/s10499-023-01108-5</a></li> <li>Zhang W, Yu C, Yin S, Chang K, Chen K, Xing Y, Yang Y, Transmission and retention of antibiotic resistance genes (ARGs) in chicken and sheep manure composting, Bioresource Technology, 382, 129190, 2023. <a href="https://doi.org/10.1016/j.biortech.2023.129190">https://doi.org/10.1016/j.biortech.2023.129190</a></li> <li>Wang Z, Yu X*, Zhao W, Wang Y, Li S, Yu C, Dong X, 3D printability of sturgeon paste as affected by colloid milling. Journal of Food Engineering, 346, 111429, 2023, <a href="https://doi.org/10.1016/j.jfoodeng.2023.111429">https://doi.org/10.1016/j.jfoodeng.2023.111429</a></li> <li>Chen KS, Yu C, Cai L, Zhang W, Xing Y, Yang Y, Bacterial community succession in aerobic-anaerobic-coupled and aerobic composting with mown hay affected C and N losses, Environmental Science and Pollution Research, 2023, <a href="https://doi.org/10.1007/s11356-023-27572-3">https://doi.org/10.1007/s11356-023-27572-3</a></li> <li>He Q*, Tong TJ*, Yu C and Wang Q, Advances in Algin and Alginate-Hybrid Materials for Drug Delivery and Tissue Engineering, Marine Drugs, 21(1), 14, 2023, <a href="https://doi.org/10.3390/md21010014">https://doi.org/10.3390/md21010014</a></li> <li>Leng L, Zou H, Wang Y, Yu C, Qi H, Seaweed Slurry Improved Gel Properties and Enhanced Protein Structure of Silver Carp (Hypophthalmichthys molitrix) Surimi, Foods, 11(19), 3115, 2022, <a href="https://doi.org/10.3390/foods11193115">https://doi.org/10.3390/foods11193115</a></li> <li>Liu Y*, Wang Z, Huang Y, Konno K, Tong T*, Yu C, Zhu B, Dong X, Characteristic stable structure of the myosin rod in dark muscle of yellowtail kingfish (Seriola aureovittata), International Journal of Food Science and Technology, 2022, <a href="https://doi.org/10.1111/ijfs.16146">https://doi.org/10.1111/ijfs.16146</a></li> </ol>

Impact Statements

Our research has increased our understanding of the effects of HHP on enzyme stability from the structural perspective. This new knowledge will serve as the foundation for the stabilization of glucose and alcohol bio-sensors.

Date of Annual Report: 10/21/2024

Report Information

Annual Meeting Dates: 07/17/2024 - 07/18/2024
Period the Report Covers: 10/01/2023 - 09/30/2024

Participants

Jose I Reyes-De-Corcuera (Florida), Jeong-Yeol Yoon (Arizona), Chenxu Yu (Iowa), Olga Tsyusko and Jarad Cochran (Kentucky), Ranee Anderson and Sam Nugen (Cornell), Evangelyn Alocilja (Michigan), Mengshi Lin (Missouri), Yi-Cheng Wang (Illinois), Sundaram Gunasekaran (Wisconsin), Anhong Zhou (Utah)

Brief Summary of Minutes

Station reports were delivered from each participant describing completed and on-going research.

Administrative updates by Hongda Chen and Amy Gruden (on behalf of Steve Lommel). Hongda Chen gave an overview of NIFA investments in nanotechnology R&D and education from 2003 through 2024, and fiscal 2025 plans and priorities including Nanotechnology for Agricultural and Food (Priority A1511) and Social Implications of Food and Agricultural Technologies (Priority A1642). He also highlighted key NIFA accomplishments for National Nanotechnology Initiative (NNI) goals, particularly Goal#4, 2, 3. He covered the Gordon Research Conference (GRC) – Nanoscale Science and Engineering for Agriculture and Food Systems on June 22-28, 2024, which was chaired by NC1194 member Dr. Carmen Gomes from Iowa State University. Finally, he gave some thoughts for NC-1194 discussion during the meeting, including recruiting initiative to bring more potential new members, exploring attending other multistate projects (e.g., S1077 Enhancing Microbial Food Safety by Risk Analysis) to discuss potential collaborations, approaches for commercialization of nano sensors, and enhancing research on micro- and nanoplastics. He suggested future meeting in conjunction with other professional meetings, such GRC IBE, and so on.

Amy Gruden reminded that this NC-1194 project expires September 2026, and it typically takes one year to start the preparation of renewal submission. Amy passed Steve Lommel’s message to remind the election of new officers. Steve encouraged NC-1194 group to resubmit the application of the North Central Regional Association’s (NCRA) Multistate Research award in the coming year. Steve also encouraged the NC-1194 to work with other multi-state group, especially food safety group.

The meeting was organized by current Chair Chenxu Yu and completed the election of officers for the 2024-2025 starting immediately after the meeting: Anhong Zhou as the Chair, Zhengrong Gu as the Vice-Chair, Yi-Cheng Wang as the Secretary. The remainder of the meeting was devoted to discussing options for 2025 meeting (tentatively August 11-12 in Salt Lake City, UT), collaborations, joint publications, grant applications, and exploring collaboration with another multistate project S1077 “enhancing microbial food safety by risk analysis”. and rewrite a conference proposal co-sponsored by NC1194 and S1077 for NIFA. Full minutes are available at NIMSS website under Reports.

Lommel, Steve (slommel@ncsu.edu) – North Carolina State University / project administrator; Chen, Hongda (hongda.chen@usda.gov) – USDA-NIFA.

Accomplishments

Contributions to the objectives of this project on its first year are summarized. At least 49 publications last year associated to the research and listed and the end of this report were published by the NC-1194 group. <ol> <li>Develop new technologies for characterizing fundamental nanoscale processes and fabricate self-assembled nanostructures</li> </ol> Outputs: Spectroscopic imaging methods for analyzing protein-nanoplastics interactions were developed, and methods to better understand the soil amendment mechanisms to reduce continuous cropping obstacle for potato were applied (IA). SERS-based nanosensor was developed by molecular self-assembly approaches to detect four pesticides, thiabendazole, thiram, endosulfan, malathion, at the limits of detection (LOD) of 44-88 µg/kg strawberry extract (MO). Nanocarriers for drug/vaccine delivery and functional food with enhanced nutritional values and quality were developed (IA). Novel nanoparticles-based adjuvants for vaccine to treat porcine epidemic diarrhea virus was developed (VT). Electrospun nanofiber-based electrochemical biosensor was developed to detect multiple agriculture related targets (WI). A novel electrochemical biosensor platforms using graphene coated magnetic nanoparticles or microfluidic chips were designed and fabricated for detection of infectious pathogens such as wine influenza A and penicillin-resistant Salmonella with rapid response (< 30 min), high sensitivity (10-19 mol/L, equal to 100 pathogen/ml) and fast detection (< 30 min) of the target DNA or RNA of pathogens (SD). <ol start="2"> <li>Develop devices and systems incorporating nanotechnology and data-driven analytics for detection of biological/chemical targets, with an emphasis on detection of infectious diseases in plants, animals, humans, and the environment</li> </ol> Outputs: Research on the characterization and understanding of environmental health toxicity and toxicity mechanisms of the various nanomaterials used in agriculture before and after environmental transformations. Transformation, interaction, and toxicity of emerging 2D nanomaterials free-standing and embedded onto nanocomposite membranes for PFAS degradation were explored. Zn- and Fe-dopped hydroxyapatite nanoparticles for nitrogen delivery was developed in a safety-by-design approached (KY). Methods to better understand and improve the mechanisms of spreading of antibiotic-resistant genes during manure composting were developed (IA). Portable gene-based and immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva were developed (IA). First generation phosphate sensors based on stimulus-response nanobrush electrodes or on carbon nanoparticles, nitrogen sensors based on laser inscribed graphene electrodes, and Listeria spp. as well as Salmonella sensors were developed (IA). Sensor-based technologies were developed to monitor antibiotic-resistant bacteria during fresh produce production using Municipal Wastewater Effluents (IA). The concentration of nano/microplastics was quantified in 132 Iowa lakes, identified the impact of the presence of bioturbators (e.g. midge fly larvae) on the behavior and distribution of microplastics in aquatic ecosystems, and assessed the impact of nanoplastics on the development of Drosophila (IA). The team in MSU developed and validated the SMART biosensor to rapidly extract and detect foodborne pathogens, such as Salmonella enterica, E. coli O157:H7, Staphylococcus aureus, and Bacillus cereus from large-volume complex food and farm samples, such as turkey and chicken meats, rinsates from turkey and chicken processing plants, poultry manure, and cattle manure. They also developed the African Swine Fever Biosensor to detect ASF in farm settings rapidly. The ASF biosensor is being validated in ASF-infected farm samples by our international collaborators (MI). The Utah State team investigated cellular oxidative stress in response to polystyrene (PS) nanoparticles (PS50 for 50 nm, PS500 for 500 nm, and PS100-NH2 for 100 nm with amino group (UT). A novel concept of using a set of biomolecules and subsequent ML classification has been proposed and successfully demonstrated for detecting environmental toxicants, including microplastics, nanoplastics, and PFAS, from various environmental samples (aerosols, particulate matter, soil, and wastewater). This method was also demonstrated for identifying the lung disease biomarker (eNAMPT) from human blood plasma samples. A smartphone-based multi-spectral fluorescence imaging system was designed, fabricated, and demonstrated for detecting microalgae from seawater and microbiota from field soil samples (AZ). A CRISPR-based biosensor was developed to detection of antimicrobial-resistant genes in Carbapenem-resistant Enterobacterales (IL). The engineering of bacteriophages was developed for isolation, concentration and detection of Salmonella in agriculture water (NY). <ol start="3"> <li>Advance the integration of novel sensor networks, information systems, and artificial intelligence for effective risk assessment and decision support for food security and safety</li> </ol> Outputs: A new textbook on machine learning (ML) and artificial intelligence (AI) in chemical and biological sensing published by Elsevier in 2024 was jointly edited by NC1194 members Chenxu Yu (IA) and Jeong-Yeol Yoon (AZ). A smartphone-based multi-spectral fluorescence imaging technology was developed for detection of microalgae and soil microbiota (AZ). ML-based SERSFormer technology was recently developed to highly sensitively and specifically detect pesticides in various agricultural samples (MO). Machine learning (ML) tools were created to analyze Raman spectra to determine the cellular oxidative stresses induced by exposure of nanoplastics (UT). An intelligent packaging technology for food quality monitoring was developed (IL). <ol start="4"> <li>Develop and update education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment.</li> </ol> Outputs: The Michigan state team continued hosting the 2024 GARD Forum virtually on March 14-16, 2024, with >700 registered participants from 39 countries in six continents. Technical sessions, short courses, and an Innovation Challenge were held during the forum with involvement of 35 teams that led to 6 finalists. The team also demonstrated the technology developed by them at the MSU Science Festival in April 2024. Short courses on Nanotechnology were taught (KY), course on biosensors was developed and taught (FL). A REU-site on wearable graphene-based stress biosensor development with community college and high school students starting summer 2021 continued through 2023-2024 (IA). A USDA conference grant “One Health” was awarded to the team (IA) to support the 2024 Annual Institute of Biological Engineering (IBE) conference in Atlanta, GA on September 14-15, 2024. <ol start="5"> <li>Increase the number academic-industry partnerships to help move the developed technologies to commercialization phase.</li> </ol> IA station continued working with an NSF-funded IUCRC team to improve academic-industry partnership and develop technologies for soil dynamics to promote commercialization of the technology (IA). The biosensors developed by MSU team (MI) has initiated the connections with the local turkey and chicken industry.

Publications

<ol> <li>Yan Liang, Bradley Khanthaphixay, Jocelyn Reynolds, Preston J. Leigh, Melissa L. Lim, and Jeong-Yeol Yoon, "A Smartphone-Based Approach for Comprehensive Soil Microbiome Profiling," Applied Physics Reviews, 2024, 11(3): 031412.</li> <li>Jeong-Yeol Yoon and Chenxu Yu, Editors, "Machine Learning and Artificial Intelligence in Chemical and Biological Sensing," Elsevier: Amsterdam/London/Cambridge, 2024, ISBN: 978-0-443-22001-2 (paperback), 978-0-443-22000-5 (eBook).</li> <li>Jeong-Yeol Yoon, "Chapter 10 - ML-Assisted Biosensors Utilizing a Set of Biological Polymers," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.259-274.</li> <li>Chenxu Yu and Jeong-Yeol Yoon, "Chapter 4 - ML-Assisted E-Nose and Gas Sensors," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.83-112.</li> <li>Jeong-Yeol Yoon, "Chapter 3 - Use of ML/AI in Chemical Sensors and Biosensors," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.71-81.</li> <li>Yan Liang and Jeong-Yeol Yoon, "Chapter 2 - Fundamentals of Machine Learning," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.23-70.</li> <li>Yan Liang and Jeong-Yeol Yoon, "Sensors for Blood Brain Barrier on a Chip," Vitamins and Hormones, 2024, 126, 219-240.</li> <li>Bailey C. Buchanan, Yisha Tang, Hannah A. Lopez, Nancy G. Casanova, Joe G.N. Garcia, and Jeong-Yeol Yoon, "Development of a Cloud-Based Flow Rate Tool for eNAMPT Biomarker Detection," PNAS Nexus, 2024, 3(5): pgae173.</li> <li>Chloe Thomas, Togzhan Spatayeva, Dawon Yu, Andrew Loh, Un Hyuk Yim, and Jeong-Yeol Yoon, "A Comparison of Current Analytical Methods for Detecting Particulate Matter and Micro/Nanoplastics," Applied Physics Reviews, 2024, 11(1): 011313.</li> <li>Sinyang Kim, Katelyn Sosnowski, Dong Soo Hwang, and Jeong-Yeol Yoon, "Smartphone-Based Microalgae Monitoring Platform Using Machine Learning," ACS ES&T Engineering, 2024, 4(1): 186-195.</li> <li>Tyler Hertenstein, Yisha Tang, Alexander S. Day, Jocelyn Reynolds, Patrick V. Viboolmate, and Jeong-Yeol Yoon, "Rapid and Sensitive Detection of miRNA Via Light Scatter-Aided Emulsion-Based Isothermal Amplification Using a Custom Low-Cost Device," Biosensors and Bioelectronics, 2023, 237: 115444.</li> <li>Yang J, Yu, XL, Dong XP, Yu C, Improvement of surimi gel from frozen stored silver carp, Gels, 10(6), 374, 2024, <a href="https://doi.org/10.3390/gels10060374">https://doi.org/10.3390/gels10060374</a></li> <li>Xing Y, Zhang P, Zhang W, Yu C, Luo Z, Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora, Frontiers in Microbiology, 14, 1318586, 2024, <a href="https://doi.org/10.3389/fmicb.2023.1318586">https://doi.org/10.3389/fmicb.2023.1318586</a></li> <li>Tong T, Qi Y, Bussiere L, Dhar D, Miller C, Yu C, Wang Q, Rational Design of Oral Vaccines by Gut Organoid Mucosal Models, Bioactive Materials, 2023, 30, 116-128, <a href="https://doi.org/10.1016/j.bioactmat.2023.07.014">https://doi.org/10.1016/j.bioactmat.2023.07.014</a></li> <li>Zhao M, Cao X, Wu Y, Zou S, Li Z, Lin X, Ji C, Dong L, Zhang S, Yu C, Liang H, Effects of prebiotics on the fermentation of traditional suancai of Northeast China, Food Science and Human Wellness, 2023, <a href="https://doi.org/10.26599/FSHW.2022.9250114">https://doi.org/10.26599/FSHW.2022.9250114</a></li> <li>Zhang W, Yu C, Yin S, Chang K, Chen K, Xing Y, Yang Y, Transmission and retention of antibiotic resistance genes (ARGs) in chicken and sheep manure composting, Bioresource Technology, 382, 129190, 2023,. <a href="https://doi.org/10.1016/j.biortech.2023.129190">https://doi.org/10.1016/j.biortech.2023.129190</a></li> <li>He Q, Habib F, Tong T, Yu C, Raman Spectroscopy for Detection of Foodborne Pathogens, Chemical Contaminants and Nanoparticles, in Encyclopedia of Food Safety, edited by Byron Brehm-Stecher, Elsevier, 2024.</li> <li>Roque JV, Pola CC, Terra LR, Oliveira TV, Teofilo RF, Gomes CL, Soares NFF, Mapping the Distribution of Additives Within Polymer Films Through Near-Infrared Spectroscopy and Hyperspectral Imaging, in Food Packaging Materials: Current Protocols, pp 183-203. 2024</li> <li>Qian H, Moreira G, Vanegas D, Tang Y, Pola C, Gomes C, McLamore E, Bliznyuk N, Improving high throughput manufacture of laser-inscribed graphene electrodes via hierarchical clustering, Scientific Reports, 1, 7980, 2024</li> <li>Beata M Szydlowska, Cícero C Pola, Zizhen Cai, Lindsay E Chaney, Janan Hui, Robert Sheets, Jeremiah Carpenter, Delphine Dean, Jonathan C Claussen, Carmen L Gomes, Mark C Hersam, Biolayer-Interferometry-Guided Functionalization of Screen-Printed Graphene for Label-Free Electrochemical Virus Detection, ACS Applied Materials & Interfaces, 16(19), 25169-25180, 2024</li> <li>Sara L Silvestre, Maria Morais, Raquel RA Soares, Zachary T Johnson, Eric Benson, Elisabeth Ainsley, Veronica Pham, Jonathan C Claussen, Carmen L Gomes, Rodrigo Martins, Elvira Fortunato, Luis Pereira, João Coelho, Green Fabrication of Stackable Laser‐Induced Graphene Micro‐Supercapacitors under Ambient Conditions: Toward the Design of Truly Sustainable Technological Platforms, Advanced Materials Technologies, 2400261, 2024</li> <li>J Claussen, J Hondred, Enhanced 3D porous architectured electroactive devices via impregnated porogens, US Patent 11,971,383</li> <li>Nathan M Jared, Zachary T Johnson, Cicero C Pola, Kristi K Bez, Krishangee Bez, Shelby L Hooe, Joyce C Breger, Emily A Smith, Igor L Medintz, Nathan M Neihart, Jonathan C Claussen, Biomimetic laser-induced graphene fern leaf and enzymatic biosensor for pesticide spray collection and monitoring, Nanoscale Horizons, 9(9), 1543-1556, 2024</li> <li>Jelena Stanković, Djuradj Milo&scaron;ević, Momir Paunović, Boris Jovanović, Nata&scaron;a Popović, Jelena Tomović, Ana Atanacković, Katarina Radulović, Davor Lončarević, Maja Raković, Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates, Water, 16(7), 962, 2024</li> <li>Khouloud Sebteoui, Djuradj Milo&scaron;ević, Jelena Stanković, Viktor Baranov, Boris Jovanović, Stefan Krause, Zoltán Csabai, Beneath the surface: Decoding the impact of Chironomus riparius bioturbation on microplastic dispersion in sedimentary matrix, Science of the Total Environment, 919, 170844, 2024</li> <li>Alyssa M Hohman, Rachel Sorenson, Boris Jovanovic, Elizabeth McNeill, The heart of plastic: utilizing the Drosophila model to investigate the effects of micro/nanoplastics on heart function, Frontiers in Toxicology, 6, 1438061, 2024</li> <li>Rachel M Sorensen, Dimitrija Savić-Zdravković, Boris Jovanović, Changes in the wing shape and size in fruit flies exposed to micro and nanoplastics, Chemosphere, 363, 142821, 2024.</li> <li>Ghazy A, Nyarku R, Faraj R, Bentum K, Woube Y, Williams M, Alocilja E, and Abebe W. 2024. Gold Nanoparticle-based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from bovine fecal Samples, Microorganisms, 12, 1069. </li> <li>Caliskan-Aydogan1 O, Zaborney Kline C1, and Alocilja EC3. 2024. Cell Morphology as Biomarker of Carbapenem Exposure, Journal of Antibiotics, 77, pages 600–611 (2024).</li> <li>Caliskan-Aydogan1 O and Alocilja EC3. 2024. A Parallel biosensor platform for the detection of carbapenemase-producing coli in spiked food and water samples, Food Control, Vol. 163, 110485.</li> <li>Caliskan-Aydogan O1, Sharief S1, and Alocilja EC3. 2023. Rapid Isolation of Low-level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles, Biosensors 2023, 13(10), 902.</li> <li>Caliskan-Aydogan O1 and Alocilja EC3. 2023. A Review of Carbapenem Resistance in Enterobacterales and Its Detection Techniques. Microorganisms, 2023, 11(6), 1491.</li> <li>Boodoo C1, Dester E1, David J1, Patel V1, Rabin KC, and Alocilja EC3. 2023. Multi-Probe Nano-Genomic Biosensor to Detect aureus from Magnetically-Extracted Food Samples, Biosensors, 2023, 13(6), 608, https://doi.org/10.3390/bios13060608.</li> <li>Sharief S1, Caliskan-Aydogan O1, Alocilja EC3. 2023. Carbohydrate-coated nanoparticles for PCR-less genomic detection of Salmonella from fresh produce, Food Control, Vol. 150, 109770.</li> <li>Boodoo C1, Dester E1, Sharief S1, and Alocilja EC3. 2023. Influence of Biological and Environmental Factors in the Extraction and Concentration of Foodborne Pathogens using Glycan-Coated Magnetic Nanoparticles, Journal of Food Protection, 86(4):100066.</li> <li>Sharief SA1, Caliskan-Aydogan O1, and Alocilja EC3. 2023. Carbohydrate-coated nanoparticles for point-of-use food contamination testing, Biosensors and Bioelectronics: X, 13 (2023), 100322, 9 pp.</li> <li>Bhattarai RK1,3, Basnet HB, Dhakal IP, Alocilja E. 2023. Virulence genes of avian pathogenic Escherichia coli isolated from commercial chicken in Nepal, Comparative Immunology, Microbiology and Infectious Diseases, 95 (2023) 101961.</li> <li>Caliskan-Aydogan O1, Sharief S1, and Alocilja EC3. 2023. Nanoparticle-Based Plasmonic Biosensor for the Unamplified Genomic Detection of Carbapenem-Resistant Bacteria, Diagnostics, 2023, 13(4), 656.</li> <li>Hajikhani, M., Hegde, A., Snyder, J., Cheng, J., Lin, M. 2024. Integrating transformer-based machine learning with SERS technology for the analysis of hazardous pesticides in spinach. Hazard. Mater. 470, 134208.</li> <li>Hajikhani, M., Kousheh, S., Lin, M. 2024. Design of a novel SERS substrate by electrospinning for the detection of thiabendazole in soy-based foods. Food Chem. 436, 137703.</li> <li>Zhai, K., Sun, L., Nguyen, T., Lin, M. 2024. Facile synthesis of gold nanostars for duplex detection of pesticide residues in grapes using SERS. Food Sci. 89, 2512-2521.</li> <li>Alsammarraie, F. K., Lin, M., Mustapha, A. 2023. Green synthesis of silver nanomaterials and evaluation of their antibacterial and antioxidant effectiveness in chicken meat. Food Biosci. 56, 103332.</li> <li>Hajikhani, M., Zhang, Y., Gao, X., Lin, M. 2023. Advances in CRISPR-based SERS detection of food contaminants: A review. Trends Food Sci. Technol. 138, 615-627.</li> <li>Wang, W., Yu, Z., Lin, M., Mustapha, A. 2023. Toxicity of silver nanoparticle incorporated-bacterial nanocellulose to human cells and intestinal bacteria.  J. Biol. Macromol. 241, 124705.</li> <li>Weng, Z., You, Z., Li, H., Wu, G., Song, Y., Sun, H., Fradlin, A., Neal-Harris, C., Lin, M., Gao, X., Zhang, Y. 2023. CRISPR-Cas12a biosensor array for ultrasensitive detection of unamplified DNA with single-nucleotide polymorphic discrimination. ACS Sensors. 8(4), 1489-1499.</li> <li>M Liang, L Yuan, C Shao, X Zheng, Q Song, Z Gu, S Lu; 2023, Sequential Visual Sensing of H2O2 and GSH Based on Fluorescent Copper Nanoclusters Incorporated Eggshell Membrane, IEEE Sensors Journal (accepted)</li> <li>Liang, T. Schaffer, A. Sobhan, M. Biesecker, Z. Yang, C. Han, J. Hu, A. Smirnova, Z. Gu, 2023, 3D Cu Pyramid Array Grown on Planar Cu Foil for Stable and Dendrite–Free Lithium Deposition, Materials Science Vol.29 No.4, 2023 (accepted)</li> <li>Y Liang, W Ding, B Yao, F Zheng, A Smirnova, Z Gu, 2023, Mediating Lithium Plating/Stripping by Constructing 3D Au@ Cu Pentagonal Pyramid Array, Batteries 9 (5), 279</li> <li>Yuanzhi Bian,* Debra L. Walter,* Chenming Zhang. Efficiency of interferon-γ in activating dendritic cells and its potential synergy with toll-like receptor agonists. Viruses (MDPI). 2023, 15, 1198. doi.org/10.3390/v15051198.</li> </ol>

Impact Statements

The research carried out by the group increased the understanding of nanotechnology and biosensors by the general public by bringing greater awareness of their current and potential roles in food and agriculture. At least 6 postdocs, 28 PhD students, 9 MS students, 25 undergraduate students, and 1 visiting scholar participated in this project. 1. Fabrication of high-performance substrates for SERS via electrospinning with self-assembly was achieved to successful detection of pesticides in foods with improved sensitivity and specificity (MO). 2. Several cross-country and cross-continent collaborations were formed through the GARD. The GARD-Asia-Africa Initiative (GARD-AAI) conducted workshops on nanotechnology and biosensors attended by scientists from both continents (MI). 3. We trained four female undergraduates and two minority students under the Summer Research Opportunity Program (SROP), a gateway to graduate education at Big Ten Academic Alliance universities, whose goal is to increase the number of underrepresented students to pursue graduate study and research careers. We also trained one minority student under the Engineering Summer Undergraduate Research Experience (EnSURE), an "internship in graduate school" program that provides participants an early opportunity to participate in research by working with faculty mentors. These students presented their work at the 2024 Mid-SURE event, where they learned the skills of public engagement and articulated the potential impact of their work on society’s real needs (MI). We demonstrated our Raman measurement of living cells exposed to nanoplastics to four native American college students (UT). 4. We also demonstrated our Salmonella biosensor to staff from the Michigan Turkey Producers and the Miller Poultry. These stakeholders received attention to nanotechnology-enabled biosensors that are capable of rapidly inform and control Salmonella contamination in turkey and chicken production systems (MI). 5. Study of adjuvants that can enhance the efficacy of nanovaccines would greatly reduce porcine epidemic diarrhea virus (PEDV) (VT). 6. Our research has increased our understanding of the effects of HHP on enzyme stability from the structural perspective (FL). This new knowledge will serve as the foundation for the stabilization of enzyme-based biosensors. 7. We designed, developed, and tested a smartphone-based multi-spectral fluorescence imaging system for detection of microalgae from seawater and microbiota from field soil samples (AZ) 8. We developed different biosensor technologies and methods for micro- and nanoplastics (including PFAS) detection and cells-MNPs interaction induced toxicity (IA, AZ, UT, KY) 9. We developed and validated novel electrochemical biosensor platforms that use graphene coated magnetic nanoparticles or microfluidic chips to detect DNA of specific microbes (SD) Outcomes Participants at MSU Received funding from the Michigan Alliance for Animal Agriculture on “Optimizing a nano-biosensor for rapid detection of the African swine fever and from the MSU AgBioResearch for a project on “Development of a Biosensing System for Rapid and Integrated Genome-to-Phenome Antimicrobial Resistance Testing (BRIGHT)”. AZ group received university’s One Health Initiative for a project entitled “Machine learning- and paper microfluidic-based classification of nanoplastics and identification of biologically interacting molecules from soil, plant, animal, and human samples”. Through an NSF funded IUCRC grant, the team from IA is collaborating with the researchers from Univ. of Florida, Univ. of Connecticut, Univ. of Washington, and Univ. of South California to develop soil dynamics technologies. UT team received funding from USDA-ARS Forage and Range Research Laboratory (FRRL) to conduct chemical composition analysis of wheat lines with salt tolerance. The SD team received funding from NASA-EPSCoR, NSF-EPSCOR, Sun Grant (North Central Center), South Dakota Oilseeds Initiative, South Dakota Oilseeds Council, South Dakota Beef Industrial Council, and USDA-NIFA.