WERA89: Potato Virus and Virus-Like Disease Management

(Multistate Research Coordinating Committee and Information Exchange Group)

Status: Active

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SAES-422 Reports

Annual/Termination Reports:

[06/16/2022] [06/27/2023] [05/15/2024]

Date of Annual Report: 06/16/2022

Report Information

Annual Meeting Dates: 03/15/2022 - 03/16/2022
Period the Report Covers: 10/01/2021 - 09/30/2022

Participants

On-line:
David Douches
Hanu Pappu
Jason Ingram
Jonathan Whitworth
Joseph Coombs
Kylie Swisher Grimm
Max Feldman
Melanie Filiatrault
Steve Hystad
Tami Brown
Alan Westra
Prabu Gnanasekaran
Mathuresh Singh
Richard Manasseh
Hira Kamal
Kiwamu Tanaka
Gregory Elison
Brooke Babler
Rich Novy
Noelle Anglin In-person:
Adam Winchester
Alexander Karasev
Alice Pilgeram
Amy Charkowski
Andrew Houser
Brian Charlton
Erik Wenninger
Jen Rushton
John Mizicko
Julie Pasche
Kasia Duellman
Kenneth Frost
Kent Sather
Lynn Woodell
Mark Pavek
Nathan Gelles
Nina Zidack
Nora Olsen
Rabecka Hendricks
Renee Rioux
Sarah Hensley
Teresa Almeida
Vamsi Nalam
Vidyasagar Sathuvalli
Yuan Zeng

In-person:
Adam Winchester
Alexander Karasev
Alice Pilgeram
Amy Charkowski
Andrew Houser
Brian Charlton
Erik Wenninger
Jen Rushton
John Mizicko
Julie Pasche
Kasia Duellman
Kenneth Frost
Kent Sather
Lynn Woodell
Mark Pavek
Nathan Gelles
Nina Zidack
Nora Olsen
Rabecka Hendricks
Renee Rioux
Sarah Hensley
Teresa Almeida
Vamsi Nalam
Vidyasagar Sathuvalli
Yuan Zeng

Brief Summary of Minutes

Minutes Attachment:

WERA89 Minutes2022.docx

Accomplishments

The group continued to conduct research, extension programs and other outreach related to potato viruses and virus-like organisms. For example:   An SCRI grant, led by Dr. Alex Karasev at the University of Idaho, was awarded in fall of 2021. Numerous collaborators across multiple states have made progress in four areas and continue efforts to develop management solutions for necrotic viruses (PMTV and PVY) that infect potato. Work continues to understand the factors affecting the spread of PVY. Advances have been made in reducing virus levels in New Brunswick in recent years. Major trends in PVY spread were identified via amassing a very large data set of grower practices and other factors. Management decisions by individual growers play a large role, among other factors. Led by Dr. Mathuresh Singh, this effort may provide other regions with strategies to improve PVY management. Research conducted in Idaho and New York to determine the interaction between cultivar and PVY strain in both the foliage and tubers will aid in certification efforts. A poster detailing the results is publicly available by contacting Jonathan Whitworth. Dr. Hanu Pappu’s group is working on several aspects of PVY including virus-induced metabolic adjustments, PVY-coded NIa protein modulating the auxin-signaling pathway and disease development, and the roles of PMTV proteins using a PVX-based expression system. Advances in dormant tuber testing for PVY (potato virus Y) continue. However, the industry is not yet at the point of replacing seed-lot grow outs, particularly since dormant tuber testing cannot efficiently detect chemical injury and variety mix. Continued advances in tuber testing methods will shift the metric of a good test beyond what has always been the first hurdle of the “false negative rate” and how we plan for beyond that. Stakeholders have requested more information that could be collected using expanded dormant tuber tests to aid in farm decision making. SHERLOCK (specific high-sensitivity reporter unlocking) is being investigated for PVY detection. This method combines recombinase polymerase amplification (RPA) with a second by cleavage of a reporter probe by a Cas13 enzyme to reduce false negative results. Environmental sensors are being investigated to improve the ability to predict powdery scab. Sensors were placed in commercial potato fields across five states and environmental / crop data were correlated to disease incidence and severity. Principle component analysis was used on soil characteristics. Root gall formation was correlated to root susceptibility and initial pathogen density.

Publications

Combest, M.M., Moroz N., Rogan C., Tanaka K., Anderson J., Rakotondrafara A.M., and Goyer A. 2021. StPIP1, a predicted PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with potato virus Y. Journal of Experimental Botany (In Press) https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erab078/6157931 Dahan, J., Cooper, W.R., Munyaneza, J., and Karasev, A.V. (2022) A new Bactericera cockerelli picorna-like virus identified in populations of potato psyllids. Archives of Virology 167: 177-182 (https://doi.org/10.1007/s00705-021-05281-x). Dahan, J., Wenninger, E.J., Thornton, M., Reyes-Corral, C.A., Olsen, N., and Karasev, A.V. (2021) Haplotyping the potato psyllid (Hemiptera: Triozidae), and the associated pathogenic bacterium ‘Candidatus Liberibacter solanacearum’ in non-crop alternative hosts in Southern Idaho. Environmental Entomology 50: 382–389 (<a href="https://doi.org/10.1093/ee/nvaa179">https://doi.org/10.1093/ee/nvaa179</a>). Elison, G.L., Novy, R.G. & Whitworth, J.L. Russet Potato Breeding Clones with Extreme Resistance to Potato Virus Y Conferred by Rychc as well as Resistance to Late Blight and Cold-Induced Sweetening. Am. J. Potato Res. 98, 411–419 (2021). <a href="https://doi.org/10.1007/s12230-021-09852-1">https://doi.org/10.1007/s12230-021-09852-1</a> Goyer A (2021) From tolerant to sensitive: how a small peptide produced by potato plants determines the fate of potato-PVY interaction. Potato Progress. Vol. XXI, Number 4. Mondal, S., Ghanim, M., Roberts, A., and Gray, S. M. 2021. Different potato virus Y strains frequently co- localize in single epidermal leaf cells and in the aphid stylet. Journal of General Virology. 102:001576 DOI 10.1099/jgv.0.001576 Mondal, S., Wintermantel, W. M., and Gray, S. 2022. Infection dynamics of potato virus Y isolate combinations in three potato cultivars. Plant dis. https://doi.org/10.1094/PDIS-09-21-1980-RE Olsen, N. and A. Karasev. 2021. Going viral- tackling the problem of evolving viruses. Potato Grower Magazine. May 50(5):36-37. Olsen, N., A. Karasev, J. Miller and J. Whitworth. 2022. Everything you wanted to know about potato viruses, but were afraid to ask. Idaho Potato Conference. Pocatello, ID. January 19, 2022. Olsen, N. 2021. Effective practices to manage Black Spot Bruise and PVY in potatoes and the proper use of products for good storage management CHS Bingham Cooperative Grower Meeting (virtual). March 4, 2021. Olsen, N. and J. Miller. 2021. Powdery scab and potato mop-top virus (PMTV) management. Miller Research Potato Pest Management seminar (virtual). February 18, 2021. Pandey, B., Mallik, I., Arjarquah, A., Shahoveisi, F., and Pasche, J. S. 2022. Assessing optimum timing for detecting Potato mop-top virus (PMTV) in potato seed tubers. Plant Dis. (abstract) in press. Reyes-Corral, C., Cooper, W.R., Horton, D., Miliczky, E., Riebe, J., Waters, T., Wildung, M., and Karasev, A.V. (2021) Association of Bactericera cockerelli (Hemiptera: Triozidae) with the perennial weed Physalis longifolia (Solanales: Solanaceae) in the potato-growing regions of western Idaho. Environmental Entomology 50: 1416-1424 Rodriguez-Rodriguez, M., Quintero-Ferrer, A., Green, K.J., Robles-Hernandez, L., Gonzalez-Franco, A.C., and Karasev, A.V. (2021) Molecular and biological characterization of recombinant isolates of Potato virus Y circulating in potato fields in Mexico. Plant Disease 105: 2688-2696 (https://doi.org/10.1094/PDIS-10-20-2215-RE). Ross, B.T., Zidack, N., and Flenniken, M.L., Extreme Resistance to Viruses in Potato and Soybean, (2021), Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.658981. Ross, B.T., Zidack, N., McDonald, R., and Flenniken, M.L. 2022. Transcriptome and Small RNA Profiling of Potato Virus Y Infected Potato Cultivars, Including Systemically Infected Russet Burbank. Viruses. 14(3), 523; https://doi.org/10.3390/v14030523 Tran, L., K. Green, M. Rodriguez-Rodriquez, G. Orellana, C. Funke, O. Nikolaeva, A. Quintero-Ferrer, M. Chikh-Ali, L. Woodell, N. Olsen and A. Karasev. 2021 (online Oct 2021). Prevalence of recombinant strains of potato virus Y in seed potato planted in Idaho and Washington states between 2011 and 2021. Plant Disease. https://doi.org/10.1094/PDIS-08-21-1852-SR. Vales, M.I., Scheuring, D.C., Koym, J.W. et al. Vanguard Russet: A Fresh Market Potato Cultivar with Medium-Early Maturity and Long Dormancy. Am. J. Potato Res. (2022). <a href="https://doi.org/10.1007/s12230-022-09877-0">https://doi.org/10.1007/s12230-022-09877-0</a> Whitworth, J.L., Gray, S.M., Ingram, J.T. et al. Foliar and Tuber Symptoms of U.S. Potato Varieties to Multiple Strains and Isolates of Potato virus Y. Am. J. Potato Res. 98, 93–103 (2021). <a href="https://doi.org/10.1007/s12230-020-09820-1">https://doi.org/10.1007/s12230-020-09820-1</a> Woodell, L. and N. Olsen. 2021. The impact of Tobacco Rattle Virus on Potato Quality at Harvest and in Storage. American Journal of Potato Research (abstract). In press. Zhang, C., Zarka, K.A., Zarka, D.G. et al. Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties. Am. J. Potato Res. 98, 42–50 (2021). https://doi.org/10.1007/s12230-020-09815-y

Impact Statements

Presentations on current research were provided by attendees. Over-arching categories for updates included virus-like organisms, diagnostics, TRV/PMTV, PVY biology, PVY management, and other topics. These updates generated good discussion and showed progress in areas such as dormant tuber testing, sampling strategies, PVY strain composition and spread, PVY management with mineral oils and biologicals, tools to breed for resistance, variety reactions to PMTV, powdery scab/PMTV prediction modeling, and others.

Date of Annual Report: 06/27/2023

Report Information

Annual Meeting Dates: 03/16/2023 - 03/17/2023
Period the Report Covers: 01/01/2022 - 12/31/2022

Participants

Attendee Institution
Adam Winchester North Dakota State University Seed Certification
Alex Karasev University of Idaho
Alex Scalzo USDA-ARS
Alice Pilgrim Montana State
Amer Fayad USDA-ARS
Amy Charkowski Colorado State University
Andrew Houser Colorado State University Potato Seed Certification
Andrew Plant Maine Seeed Certification
Aymeric Goyer Oregon State University
Brooke Babler Wisconsin Seed Certification
Chakradhar Mattupalli Washington State University
Chris McIntosh University of Idaho
Emma Tracy Seed Certification Montana
Erik Wenninger University of Idaho
Esra Yilmaz Colorado State University
Jennifer Dillinger Nebraska Seed Certification
Jennifer Rushton Colorado State University
Jessica Chitwood-Brown Colorado State University
Joe Coombs Michigan State
Jonathan Whitworth USDA-ARS
Josie Spurgeion Wisconsin Seed Certification
Julie Pasche North Dakota State University
Kasia Duellman University of Idaho
Keith Schunetz Agdia
Kelie Yoho University of Idaho
Ken Frost Oregon State University
Kent Sather North Dakota State University Seed Certification
Kylie Swisher-Grimm USDA-ARS
Lisa Tran University of Idaho
Mahbuba Fatema Colorado State University
Mark Pavek Washington State University
Mathuresh Singh Seed Certification New Brunswick
Max Feldman USDA-ARS
Melissa Bertram University of Idaho
Michelle Leckler Colorado State University Potato Seed Certification
Mohamad Chickh-Ali Colorado State University
Nathan Gelles University of Idaho
Presely Mosher North Dakota State University
Rabecka Hendricks University of Idaho
Russel Groves University of Wisconsin
Tara Gauthier Agdia
Teresa Almeida Colorado State University Potato Seed Certification
Vidyasagar Sathuvalli Oregon State University
Walter DeJong Cornell University

Brief Summary of Minutes

Minutes Attachment:

WERA Meeting Minutes 031623-Report.docx

Accomplishments

Publications

Journal Publications Chikh-Ali, M. and Karasev, A.V. 2023. Chapter 11. Virus diseases of potato and their control. In: Potato Production Worldwide (Eds., Caliskan, M.E., Bakhsh, A., and Jabran, K.), Elsevier, Inc.: London, San Diego, Cambridge, Oxford; pp. 199-211.   Ding, P., Chen, D., Feng, H., Li, J., Cao, H., Muning, T., Li, J., Hao, X., Han, P., Karasev, A.V., and Feng, X. 2022. Prevalence and strain composition of potato virus Y circulating in potato fields in China’s north-central province of Shanxi. Plant Disease 106: 1434–1445 (<a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.1094%2FPDIS-09-21-1950-RE&data=05%7C01%7C%7C52b664a18fe3469760c908db68372135%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C638218358214345045%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=do3T5e17HDLPLwuMSQd3szsxn%2BJD6ILhD8CE6ztPR9I%3D&reserved=0">https://doi.org/10.1094/PDIS-09-21-1950-RE</a>).   Duellman, K.M.*, Whitworth, J., Lent, M.A., Bertram, M.C., and Randall, J.C. Published online ahead of print 19 June 2022. Mechanical transmission of Potato virus Y by seed cutting is not a contributing factor to increased virus in field production. Plant Health Progress. <a href="https://doi.org/10.1094/PHP-02-22-0011-RS">https://doi.org/10.1094/PHP-02-22-0011-RS</a> (1, 2, 3, 4, 5, 6)    Duellman, K., Woodhall, J., Karasev, A.V., Olsen, N., and Whitworth, J.L. 2022. Potato mop-top virus: biology and disease management. University of Idaho Extension Bulletin 1017: June 2022; 5pp.    Gelles, N., Olsen, N., Thornton, M., Karasev, A., Hendricks, R., and Woodell, L. 2023. Novel methods to induce sprouting in dormant potato tubers. Abstracts of the 106th Annual Meeting of The Potato Association of America. American Journal of Potato Research 100 (2): 121.   Hendricks, R.L., N. Olsen, M. Thornton and P. Hatzenbuehler. 2022. Susceptibility of potato cultivars to blackspot and shatter bruise at three impact heights. American Journal of Potato Research 99:358-368.   Kud, J., Dahan, J., Orellana, G.E., Dandurand, L.-M., and Karasev, A.V. 2022. A novel rhabdovirus associated with the Idaho population of potato cyst nematode Globodera pallida. Viruses 14 (12): 2718 (<a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.3390%2Fv14122718&data=05%7C01%7C%7C52b664a18fe3469760c908db68372135%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C638218358214345045%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=CnKjVJGu860T1U8fu4p0YdId4jdylSwwH2u%2BbJTiBkc%3D&reserved=0">https://doi.org/10.3390/v14122718</a>).   Manasseh, R., Berim, A., Kappagantu, M., Moyo, L., Gang, D. R., & Pappu, H. R. (2023). Pathogen-triggered metabolic adjustments to potato virus Y infection in potato. Frontiers in plant science, 13, 1031629. <a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.3389%2Ffpls.2022.1031629&data=05%7C01%7C%7Cccdb8cf63f294889901008db67d660a4%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C638217942678801647%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=WRvDVP26XQP%2FnqqdE13gyRxPWQFENlHFa%2BuKMQ1Ewh4%3D&reserved=0">https://doi.org/10.3389/fpls.2022.1031629</a>   Mora, V., M. Ramasamy, M. B. Damaj, S. Irigoyen, V. Ancona, C. A. Avila, M. I. Vales, F. Ibanez, and K. K. Mandadi. 2022. Identification and characterization of new sources of zebra chip disease resistance among wild Solanum species. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2022.857493.   Novy, R.G., J.L. Whitworth, J.C. Stark, R.R. Spear, B.L. Schneider, M.J. Pavek, N.R. Knowles, L.O. Knowles, B.A. Charlton, V. Sathuvalli, S. Yilma, C.R. Brown, T.L. Brandt, M. Thornton, and N. Olsen. 2021. La Belle Russet: an early maturing, dual-purpose variety having a high percentage of marketable yield, long tuber dormancy, and a reduced incidence of sugar ends. American Journal of Potato Research 98:395-410.   Pandey, J, D.C. Scheuring, J.W. Koym, and M.I. Vales. 2022. Genomic Regions Associated with Tuber Traits in Tetraploid Potatoes and Identification of Superior Clones for Breeding Purposes. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2022.952263.   Tran, L.T., Green, K.J., Rodriguez-Rodriguez, M., Orellana, G.E., Funke, C.N., Nikolaeva, O.V., Quintero-Ferrer, A., Chikh-Ali, M., Woodell, L., Olsen, N., and Karasev, A.V. 2022. Prevalence of recombinant strains of potato virus Y in seed potato planted in Idaho and Washington states between 2011 and 2021. Plant Disease 106: 810-817 (<a href="https://gcc02.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.1094%2FPDIS-08-21-1852-SR&data=05%7C01%7C%7C52b664a18fe3469760c908db68372135%7Ced5b36e701ee4ebc867ee03cfa0d4697%7C0%7C0%7C638218358214345045%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=S6zVp95KiWXGa0DqLKoeF7zSliCHVigqHpnhg2WA2bQ%3D&reserved=0">https://doi.org/10.1094/PDIS-08-21-1852-SR</a>).   Toinga-Villafuerte, S., M.I. Vales, J.M. Awika and K.S. Rathore. 2022. CRISPR/Cas9-mediated mutagenesis of the granule-bound starch synthase gene in the potato variety Yukon Gold to obtain amylose-free starch in tubers. International Journal of Molecular Sciences. 23: 4640 DOI: 10.3390/ijms23094640.   Toinga-Villafuerte, S., M.R. Janga, M.I. Vales, and K.S. Rathore. 2022. Green fluorescent protein gene as a tool to examine the efficacy of Agrobacterium delivered CRISPR/Cas9 reagents to generate targeted mutations in the potato genome. Plant Cell, Tissue and Organ Culture. Plant Cell, Tissue and Organ Culture (PCTOC). https://doi.org/10.1007/s11240-022-02310-8.   Vales, M.I., D.C. Scheuring, J.W. Koym, D.G. Holm, S.Y.C. Essah, R.G. Wilson, J.K. Sidhu, R.G. Novy, J.L. Whitworth, J.C. Stark, R.R. Spear, V. Sathuvalli, C.C. Shock, B.A. Charlton, S. Yilma, N.R. Knowles, M.J. Pavek, C.R. Brown, D.A. Navarre, M. Feldman, C.M. Long, and J.C. Miller, Jr. 2022. Vanguard Russet: A Fresh Market Potato Cultivar with Medium-Early Maturity and Long Dormancy. American Journal of Potato Research. 99: 258–267. Hoopes, G., X. Meng, J.P. Hamilton, S.R. Achakkagari, F. de A. Freitas Guesdes. M.E. Bolger, J.J. Coombs, D. Esselink, N.R. Kaiser, L. Kodde, M. Kyriakidou, B. Lavrijssen, N. van Lieshout, R. Shereda, H.K. Tuttle, B. Vaillancourt, J.C. Wood, J. M. de Boer, P.M. Bourke, D. Douches, H.J. van Eck, D. Ellis, M.J. Feldman, K.M. Gardner, J. C.P. Hopman, J. Jiang, W.S. de Jong, J.C. Kuhl, R.G. Novy, S. Oome, V. Sathuvalli, E.H. Tan, R.A. Ursem, M.I. Vales, K. Vining, R. G.F. Visser, J. Vossen, G.C. Yencho, N.L. Anglin, C.W.B. Bachem, J.B. Endelman, L.M. Shannon, M. Strömvik, H.H. Tai, B. Usadel, C. Robin Buell, R. Finkers. 2022. Phased, chromosome-scale genome assemblies of tetraploid potato reveals a complex genome, transcriptome, and proteome landscape that underpin phenotypic diversity. Molecular Plant 15: 520-536.   Conference Abstracts da Silva, J., N. Gracia, I. Vales, S. Gautam, and K. Mandadi. Applications of bioreactors to plant breeding. 76th Annual Meeting of the Subtropical Agriculture and Environments Society. February 11, 2022, Weslaco, TX. (oral presentation)   Duellman, K.M., Lent, M.A., Liu, C., McKinney, L.F., and Wenninger, E.J. 2023. Timing of in-season plant-to-plant spread of Potato virus Y in potato in Idaho, United States. 12th International Congress of Plant Pathology, Lyon, France, August 20-25, 2023 (poster).   Gelles, N., Olsen, N., Thornton, M., Karasev, A., Hendricks, R., and Woodell, L. 2023. Novel methods to induce sprouting in dormant potato tubers. Abstracts of the 106th Annual Meeting of The Potato Association of America. American Journal of Potato Research 100 (2): 121.   Ifeduba, A.M., S. Zhen, and M.I. Vales. Global warming-ready potatoes: Understanding heat tolerance in potato clones from the Texas A&M breeding program. National Association of Plant Breeders 2022 Annual Meeting. August 8-11, 2022, Ames, IA. Poster.   Jiao, A., S. Gautam, J. Pandey, D.C. Scheuring, J.W. Koym, M.I. Vales. 2022. Genome-wide association studies for tuber dormancy in tetraploid potatoes. American Society for Horticultural Sciences. 2022 Annual Conference. Chicago, IL, July 30 - August 3, 2022. Poster.   Jiao, A., S. Gautam, J. Pandey, D.C. Scheuring, J.W.  Koym, M.I. Vales. Investigating the genetic background of potato tuber dormancy. Plant and Animal Genome Conference XXIX. January 8-12, 2022. Online. Digital poster.   Gautam S., J. Pandey, D.C. Scheuring, J.W. Koym, and M.I. Vales. Genome-wide association study on potato tuber defects under heat stress. Plant and Animal Genome Conference XXIX. January 8-12, 2022. Online. Digital poster.   Pandey, J., D.C. Scheuring, J.W. Koym, and M.I. Vales. Genomic prediction of chipping quality in tetraploid potato. Plant and Animal Genome Conference XXIX. January 8-12, 2022. Online. Digital poster.   Tran, L.T., Green, K.J., Rodriguez-Rodriguez, M., Orellana, G.E., Funke, C.N., Nikolaeva, O.V., Quintero-Ferrer, A., Chikh-Ali, M., Woodell, L., Olsen, N., and Karasev, A.V. 2023. Changes in prevalence of potato virus Y strains circulating in potato in the Pacific Northwest, 2011-2021. Abstracts of the 106th Annual Meeting of The Potato Association of America. American Journal of Potato Research 100 (2): 128.   Press Duellman, K. 2022. Border crops for Potato virus Y management in seed potato production. Potato Grower, December 2022, pp. 24-25.   Gelles, N., Olsen, N., Thornton, M., and Karasev, A. 2022. Early detection. New methods to break tuber dormancy to facilitate virus testing of seed potato. Potato Country, December 2022; pp. 20-22.   Olsen, N. Karasev, A., and Whitworth, J. 2022. Changing faces. How the industry can adjust to evolving PVY strains. Potato Grower Magazine, April 2022; pp. 30-31.   Olsen, N., Whitworth, J. and Karasev, A. 2022. New kids on the block. Success in PVY-resistant varieties. Potato Grower Magazine, May 2022; pp. 32-33.

Impact Statements

The WERA-89 meeting allows for stronger collaboration among certification programs and researchers to tailor future research projects to address problems currently being faced in potato production across the US.

Date of Annual Report: 05/15/2024

Report Information

Annual Meeting Dates: 03/12/2024 - 03/13/2024
Period the Report Covers: 01/01/2023 - 12/31/2023

Participants

In-person attendees
First name Last name Affiliation
Noelle Anglin USDA ARS
Alex Karasev University of Idaho
Nora Olsen University of Idaho
Nina Zidack Montana Seed Certification
Sagar Sathuvalli Oregon State University
Mark Pavek Washington State University
Rebecka Hendricks University of Idaho
Walter DeJong Cornell University
Jonathan Whitworth USDA ARS
Ana Cristina Fulladolsa Colorado State University
Jaime Willbur Michigan State University
Alan Westra ICIA
Rhett Spear University of Idaho
Keith Schultz Agdia
Heidi Falzon Agdia
Eric Wenninger University of Idaho
Chris McIntosh University of Idaho
Pragati Dahal University of Idaho
Cole Lubinski Wisconsin Seed Potato Certification
Alex Scalzo University of Idaho
Kelie Yoho University of Idaho
Alice Pilgeram Montana State University
Josie Spurgeon Wisconsin Seed Potato Certification
John Onditi USDA ARS/ORISE
Melanie Filiatrault USDA ARS
Adam Winchester North Dakota SSD
Andrew Houser Colorado Potato Certification Service
Sarah Hensley Colorado Potato Certification Service
Chakradhar Mattupalli Washington State University
Julie Pasche North Dakota State University
Brooke Babler Wisconsin Seed Potato Certification

Zoom attendees
First name Last name Affiliation
Amer Fayad USDA ARS
Aymeric Goyer Oregon State University
Benita Matheson Washington Seed Potato Association
Tami Brown Oregon State University
Caroline Gray Colorado State University
Eric Byre Minnesota Dept. of Agriculture
Eric Hitchcock Maine Seed Potato Certification
Jenn Dillinger Nebraska Potatoes
Joseph Coombs Michigan State University
Ken Frost Oregon State University
Kiwamu Tanaka Washington State University
Kylie Swisher Grimm USDA ARS
Lisa Tran ICIA
Mathuresh Singh Agri-Food Canada Potato Research Centre
Max Feldman USDA ARS
Teresa Almeida Colorado Potato Certification Service
Vamsi Nalam Colorado State University
Zachariah Hansen USDA ARS
Jason Ingram Cornell University
Amy Charkowski Colorado State University
Jacob Schow Montana Seed Certification
Damen Kurzer Michigan Seed Potato Association
Melinda Lent University of Idaho
Melissa Bertram University of Idaho
Mio Sato-Cruz Michigan State University
Hanu Pappu Washington State University
Karkee Manoj Washington State University
Jeffrey Endelman UW-Madison

Brief Summary of Minutes

Accomplishments

<ul> <li>The potato virus and virus-like research and extension community continued to make advancements towards the management of economically important diseases caused by these organisms. Examples include:</li> <li>A Specialty Crop Research Initiative (SCRI) was awarded in 2021. The SCRI titled Development of sustainable system-based management strategies for two vector-borne, tuber necrotic viruses in potato is led by Alexander Karasev (U of ID). Many members of the WERA89 are involved in the four specific objectives outlined in the SCRI. Discussions were started on the next steps needed to advance research and extension efforts in this area.</li> <li>The renewal for WERA89 will be submitted during the next year. Discussions were initiated concerning responsibilities for completion of the resubmission.</li> <li>Validation studies for direct tuber testing of PVY is continuing in states such as Colorado, Wisconsin, and Montana.</li> <li>Mail-away PVY test project conducted with over 40,000 tubers yielded results comparable to those obtained from winter grow outs. Efforts are in progress to build an automated tuber sampling robot that will ease tedious sampling collection process involved with direct tuber testing.</li> <li>Role of dogs in detecting PVY from tubers was found to yield inconsistent results in studies conducted at Montana and Colorado.</li> <li>Modifying irrigation to manage powdery scab and/or PMTV tuber symptoms was not effective and resulted in significant yield and quality reductions.</li> <li>Field studies from Idaho showed that seedborne PVY can impact yield, but the response is cultivar dependent and physiological age of the seed.</li> <li>Ten peer reviewed papers, eight articles in trade journals, and six abstracts focusing on necrotic potato viruses were published between Mar 2023-24.</li> </ul>

Publications

Peer-reviewed Publications: Bvindi, C. N., Howe, K., Wang, Y., Mullen, R. T., Rogan, C. J., Anderson, J., Goyer, A. J. 2023. Potato non-specific lipid transfer protein StnsLTPI.33 is associated with the production of reactive oxygen species, plant growth, and susceptibility to Alternaria solani. Plants 12:3129. <a href="https://doi.org/10.3390/plants12173129">https://doi.org/10.3390/plants12173129</a> Dahan, J., Pedroni, M.J., Thompson, B.D., Chikh-Ali, M., Dandurand, L.-M., Kuhl, J.C., and Karasev, A.V. 2023. First report of tomato chlorotic dwarf viroid infecting litchi tomato (Solanum sisymbriifolium). Plant Disease 107: 2564. <a href="https://doi.org/10.1094/PDIS-03-23-0422-PDN">https://doi.org/10.1094/PDIS-03-23-0422-PDN</a>    Dahan, J., Orellana, G.E., Wald, K.B., Wenninger, E.J., Cooper, W.R., and Karasev, A.V. 2024. Bactericera cockerelli picorna-like virus and three new viruses found circulating in populations of potato/tomato psyllids (Bactericera cockerelli). Viruses 16: 415 <a href="https://doi.org/10.3390/v16030415">https://doi.org/10.3390/v16030415</a> Daniel, J., and Chikh-Ali, M, 2024. Dynamics of potato virus Y infection pressure and strain composition in the San Luis Valley, Colorado. Plant Disease (Accepted Jan 2024. In Press). Gnanasekaran, P., Zhai, Y., Kamal, H., Smertenko, A., and Pappu, H.R. 2023. A plant virus protein, NIa-pro promotes disease development via by modulating Indole-3-acetic acid amido synthetase. Frontiers in Plant Science. 14:1112821. <a href="https://doi.org/10.3389/fpls.2023.1112821">https://doi.org/10.3389/fpls.2023.1112821</a> Goyer, A. J., Bvindi, C. N. 2023. Overexpression of VQ motif-containing gene does not affect infection rates of potato with potato virus Y. American Journal of Potato Research 100: 233–239. <a href="https://doi.org/10.1007/s12230-023-09913-7">https://doi.org/10.1007/s12230-023-09913-7</a> Goraya, M., Yan, G., Whitworth, J., and Swisher Grimm, K.D. 2023 Advancing nematode identification on potato: An isothermal recombinase polymerase amplification assay for stubby root nematode, Paratrichodorus allius. American Journal of Potato Research 101:52-64. <a href="https://doi.org/10.1007/s12230-023-09940-4">https://doi.org/10.1007/s12230-023-09940-4</a> Kamal, H., V. Lynch-Holmes, H.R. Pappu, and Tanaka, K. 2024. Starch plays a key role in sporosorus formation by the powdery scab pathogen Spongospora subterranea. Phytopathology 114:568-579. <a href="https://doi.org/10.1094/PHYTO-07-23-0224-R">https://doi.org/10.1094/PHYTO-07-23-0224-R</a> Rodriguez-Rodriguez, M., Chikh-Ali, M., Feng, X., and Karasev, A.V. (2024). Genome sequences of six recombinant variants of potato virus Y identified in North American potato cultivars grown in China. Microbiology Resource Announcements 12: e00512-23 <a href="https://doi.org/10.1128/MRA.00512-23">https://doi.org/10.1128/MRA.00512-23</a> Wenninger, E.J. and Rashed, A. 2024. Biology, ecology, and management of the potato psyllid, Bactericera cockerelli (Hemiptera: Triozidae), and zebra chip disease in potato. Annual Review of Entomology 69:139–157. <a href="https://doi.org/10.1146/annurev-ento-020123-014734">https://doi.org/10.1146/annurev-ento-020123-014734</a>   Abstracts: Arjarquah, A., Singh, J., Zitnick-Anderson, K., Pandey, B., Gill, U., and Pasche, J.S. 2023. A draft reference genome of Spongospora subterranea from North America. (Abst.) Amer J Potato Res. (In Press) Dahan, J., Pedroni, M.J., Thompson, B.D., Chikh-Ali, M., Dandurand, L.-M., Kuhl, J.C., and Karasev, A.V. 2023. An asymptomatic infection of tomato chlorotic dwarf viroid in litchi tomato (Solanum sisymbriifolium). (Abstr.) Phytopathology 113: S3.197. <a href="https://doi.org/10.1094/PHYTO-113-11-S3.1">https://doi.org/10.1094/PHYTO-113-11-S3.1</a> Gelles, N., N. Olsen, M. Thornton, A. Karasev, R. Hendricks, and Woodell, L. 2023. Alternative methods to initiate sprouting in potatoes to facilitate PVY testing (abstract). American Journal of Potato Research 100:115-134. Ingram, J.T., Mattupalli, C., Charkowski, A.O., Karasev, A.V., and Filiatrault, M.J. 2023. A grower-supported mail-away dormant potato tuber test for molecular detection of potato virus Y, and two other tuber necrotic viruses. (Abstr.) Phytopathology 113: S3.35. <a href="https://doi.org/10.1094/PHYTO-113-11-S3.1">https://doi.org/10.1094/PHYTO-113-11-S3.1</a> Kud, J., Dahan, J., Orellana, G.E., Dandurand, L.-M., and Karasev, A.V. 2023. A novel rhabdovirus associated with the Idaho population of the potato cyst nematode Globodera pallida. (Abstr.) Phytopathology 113: S3.21. <a href="https://doi.org/10.1094/PHYTO-113-11-S3.1">https://doi.org/10.1094/PHYTO-113-11-S3.1</a> Qin, R., Goyer, A. J., and Torabian, S. 2023 Effect of potassium fertilization on the nutritional contents of potato tubers. ASA, CSSA, SSSA International Annual Meeting <a href="https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/149926">https://scisoc.confex.com/scisoc/2023am/meetingapp.cgi/Paper/149926</a>    Thesis: Gelles, N. A. 2023. Altering potato tuber physiology to promote dormancy break and implications of PVY in seed certification. Master’s Thesis, University of Idaho.   Trade Publications: Duellman, K., and Wenninger, E. 2023. Tracking Potato virus Y. Potato Grower 52(13):28-29. Duellman, K., Wenninger E., and Woodhall, J. 2023. Monitoring vectors of potato pathogens to assess risk. Potato Grower 52(11):24-25. Grimm, K. S., Quick, R., Cimrhakl, L., Feldman, M., and Pavek, M. 2024. Improving our understanding of potato mop-top virus in the northwest U.S. Potato Grower 53(1):33-38. Karasev, A., and Olsen, N. 2023. Potato virus initiative developing direct tuber testing alternatives. Potato Grower 52(4):22-23. Miller, J. N. Olsen, and J. Woodhall. 2023. Can irrigation management reduce powdery scab or PMTV symptoms? Potato Progress 23(6). Schreiber, A., A. Jensen, S. Rondon, E. Wenninger, S. Reitz, and Waters, T. 2023. Integrated pest management guidelines for insects and mites in Idaho, Oregon and Washington potatoes. <a href="https://www.nwpotatoresearch.com/item/pest-management-options">https://www.nwpotatoresearch.com/item/pest-management-options</a> Zidack, N. 2023. Seed potato workshop proves highly valuable. Potato Grower 52(6):36.37. Zidack, N. 2024. Why Hawaii for seed growout? Potato Grower 53(1):90-91.         Presentations: Berrian, T., Clarke, C., and Goyer, A. J. 2023. Annual Meeting of the Potato Association of America, "Testing thiamin as an immunity inducer against diseases of potato," Charlottetown, Prince Edward Island, Canada. July 23, 2023. Duellman, K.M., Lent, M.A., Liu, C., McKinney, L.F., and Wenninger, E.J. 2023. Timing of in-season plant-to-plant spread of Potato virus Y in potato in Idaho, United States. 12th International Congress of Plant Pathology, Lyon, France, August 20-25, 2023 (poster). Gelles, N. and Olsen, N. 2023. Breaking tuber dormancy to support the seed certification process. WERA89 Meeting. Denver, CO, March 16, 2023. Gelles, N. and Olsen, N. 2023. Use of smoke in seed certification to induce sprouting. Nebraska Potato Council Meeting (virtual). January 27, 2023. Goyer, A. J., Bvindi, C., Wang, Y., and Mullen, R. T. 2023. International Society of Plant Microbe Molecular Interactions, "Functional characterization of a member of the non-specific lipid transfer protein family in potato," Providence, Rhode Island. July 17, 2023. Goyer, A. J., Combest, M., and Bvindi, C. 2023. Annual Meeting of the Potato Association of America, "Inferring gene functions from transcriptomic data: a precautionary tale," Charlottetown, Prince Edward Island, Canada. July 23, 2023. Hendricks, R. and Olsen, N. 2023. PVY and Yield- How much of an impact. WERA89 Meeting. Denver, CO, March 16, 2023. Hendrick, R. and Olsen, N. 2023. Progress on sprout promotion for direct tuber testing and the WGO, PVY impact on yield, seed size and PVY relationship, and extension activities. Potato Virus Initiative SCRI meeting. Denver, CO, March 17, 2023. Olsen, N., A. Karasev, E. Wenninger, K. Duellman, and Whitworth, J. 2024. PVY and PMTV and what we have learned from the SCRI project. Idaho Potato Conference. Pocatello, ID January 18, 2024. Olsen, N. R. Hendricks, and Karasev, A. 2023. Highlights from the Potato Virus Initiative. Idaho Association of Plant Protection Annual Meeting. Rupert, ID. November 2, 2023. Olsen, N. 2023. Potato Production and Storage Research: Emphasis on testing for PVY. Snake River Weed Management Tour and Field Day. Kimberly, ID, June 28, 2023. Olsen, N. and Karasev, A. 2023. Potato Virus Initiative: developing solutions update and discussion. Washington State University Potato Field Day. Othello, WA, June 22, 2023. Olsen, N. 2023. Diagnosing potato diseases and management options. Shoshone-Bannock Tribes ARM 29th Annual Grower Meeting. Fort Hall, ID, March 2, 2023. Olsen, N. 2023. Seed potatoes: stem numbers, seed size and PVY. Bingham Ag Services Annual Grower Meeting. Blackfoot, ID, March 1, 2023. Olsen, N. and Miller, J. 2023. Interactive storage disease diagnosis workshop. Idaho Potato Conference. Pocatello, ID January 18, 2023. Qin, R., Goyer, A. J., and Torabian, S. 2023. ASA, CSSA, SSSA International Annual Meeting, "Effect of potassium fertilization on the nutritional contents of potato tubers," St Louis, Missouri. October 31, 2023.

Impact Statements

WERA89 continued to foster a collaborative platform for both seed potato certification agencies and potato researchers to discuss topics related to current state, challenges and prospects in seed certification; pest management; breeding innovations; diagnostic testing and optimization capabilities; and improving our overall understanding of necrotic viruses of potato.