Brief Summary of Minutes of Annual Meeting

The 2021 NC229 meeting was held December 4^th, 2021 in conjunction with the NA PRRS Symposium in Chicago Marriott Downtown Magnificent Mile Hotel. The meeting has the honor to host 19 presenters from multiple stations and 37 poster presentation. The business meeting centered on the topics noted below:

• NIFA representative could not attend the meeting due to USDA restrictions.
• Dr. Rowland was approved as advisor of the NC229 group.
• Dr. Sheela Ramamoorthy from North Dakota State University completed 2-year term as chair of NC229, and Dr. Roman Pogranichniy from Kansas State University will be becoming chair of NC229 for a 2-years term.
• Dr. Pablo Pineyro (Iowa State University) assumed the position of vice-chair and Dr. Hiep Vu (Nebraska state university) secretary of the NC229 committee. 
• Andreia Arruda (The Ohio State University) was elected as the new member at large
• A short retirement ceremony was conducted in honor of Dr. Osorio led by Dr. Bob Rowland 
• Dr. Ying Fang and Dr. Bob Rowland announced the results of the vote cast by the NC229 station representatives on whether to move the meeting to a new location or date, with the majority of station reps voting for a change of venue and time. 
• The meeting was adjourned.

1. Influenza vaccine efficacy studies demonstrated that intranasal delivery of Nano-11 and Poly(I:C) based inactivated SwIAV vaccine induce polyfunctional and cross-protective cell-mediated immunity. (The Ohio State University, Purdue University)
2. PRRSV detection studies showed a higher probability of detection in processing fluids compared in breeding farms and tonsil scrapings in growing pig farms. Collaborators: The Ohio State University, North Carolina State University, and University of Minnesota).
3. We demonstrated during fetal infection, PRRSV produce an endocrine disruption, reducing animal performance. Additional studies showed that thyroid hormone levels may be promising biomarkers for genetic improvement of resilience during PRRSV challenge (ARS, USDA, Purdue University, University of Saskatchewan)
4. We demonstrated effective protection induced by an experimental subunit DIVA vaccine against PRRS Virus (University of Illinois, University of Nebraska). In addition, monoclonal antibodies were developed for a DIVA-based, diagnostic ELISA for detection of vaccinated versus naturally infected animals (USDA, (South Dakota State University)
5. Susceptibility studies demonstrated SARS-CoV2 lack of susceptibility on swine and cattle, but not in whitetail deer (USDA, Cornell University)
6. Based on a reverse genetics system we created a virulent and pathogenic infectious clone of Senecavirus A (Cornell University, South Dakota State University, Universidade Federal de Pelotas)
7. Targeting suicidal replication we created a PCV2 vaccine that enhance the safety of attenuated vaccines. (North Dakota State University, Iowa State University, South Dakota State University)
8. We development of a blocking ELISA for detection of antibodies against ASFV (University of Illinois, Kansas State University, Iowa State University)
9. Intestinal microbiota studies identified gut microbes associated with improved weight gain on pigs after immunization with PRRS MLV vaccine and co-challenge with PRRSV/PCV2, which may increase the efficacy of PRRS vaccination. (Kansas State University, Lawrence Livermore National Laboratory, Iowa State University)
10. The first United States Swine Pathogen Database platform to integrate veterinary diagnostic laboratory sequence data was create to monitor emerging pathogens of swine and to combine and compare with sequences deposited in GenBank. (ARS, Iowa State University, Kansas State University, South Dakota State University, and Cornell University)

1. Chaudhari J., Liew CS, Riethoven JJ, Sillman S., and Vu H., 2021. Porcine reproductive and respiratory syndrome virus infection upregulates negative immune regulators and T cell exhaustion markers. J Virol. 2021 Oct 13;95(21):e0105221. doi: 10.1128/JVI.01052-21. Epub 2021 Aug 11.
2. Rakibuzzaman, A.; Pineyro, P.; Pillatzki, A.; Ramamoorthy, S. Harnessing the genetic plasticity of PCV2 to target suicidal replication. Viruses 13, 1676. https://doi.org/10.3390/v13091676
3. Magnus R. Campler, Ting-Yu Cheng, Declan C. Schroeder, My Yang, Sunil K. Mor, Juliana B. Ferreira, Andréia. G. Arruda. A longitudinal study on PRRSV detection in swine herds with different demographics and PRRSV management strategies. Transboundary and Emerging Diseases. 2021 https://doi.org/10.1111/tbed.14386
4. Wang Y, Yim-im W, Porter E, Lu N, Anderson J, Noll L, Fang Y, Zhang J, Bai J. (2021). Development of a bead-based assay for detection and differentiation of field strains and four vaccine strains of type 2 porcine reproductive and respiratory syndrome virus (PRRSV-2) in the USA. Transboundary and Emerging Diseases. 68: 1414-1423.
5. Yuan F, Petrovan V, Giménez-Lirola L, Zimmerman J, Rowland RRR, Fang Y. (2021). Development of a Blocking Enzyme-Linked Immunosorbent Assay for Detection of Antibodies Against African Swine Fever Virus. Pathogens. 2021 Jun 17;10(6):760. doi: 10.3390/pathogens10060760.
6. Constance LA, Thissen JB, Jaing CJ, McLoughlin KS, Rowland RRR, Serão NVL,Cino-Ozuna AG, Niederwerder MC. Gut microbiome associations with outcome following co-infection with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in pigs immunized with a PRRS modified live virus vaccine. Vet Microbiol. 2021 Mar;254:109018. doi: 10.1016/j.vetmic.2021.109018. Epub 2021 Feb 16. PMID: 33639341.

Full list of publications in the supplementary  material section

Funding Leveraging via collaborative grants between stations and members.

1. Dr. RJ. Gourapura from The Ohio State University collaborated with Dr. D. Diel from Cornell University on: A Multi-Species Vaccine Delivery Platform for Infectious Disease Prevention and Control in Livestock. USDA-AFRI, 2017-67015-26909.
2. RJ . Gourapura and Dr. SP. Kenny from The Ohio State University, Dr. JK. Lunney and Dr. C. Loving from the USDA, and Dr. JM LaBresh collaborated on: Development of new swine reagents to broaden our understanding of immune correlates of protection and microbial pathogenesis. USDA-AFRI. ($500,000)
3. H. HogenEsch from Purdue University and Dr. RJ. Gourapura from The Ohio State University, collaborate on: Improving vaccine performance with novel phytoglycogen nanoparticle adjuvants
4. A. Arruda and M. Pairis-Garcia from The Ohio State University, Drs G. Almond and J. Ferreira from North Carolina State University, and Dr Declan Schroeder, C. Vilalta and SK. Mor from University of Minnesota collaborate on: Assessing within-herd PRRS variability and its impact on production parameters. National Pork Board. ($106,959)
5. S. Ramamoorthy, Dr. B. Webb from North Dakota State University and Dr. A. Pillatzki and from South Dakota State University collaborate on: Integrating vaccine efficacy and safety by directed suicidal replication. USDA NIFA AFRI – Animal Health and Disease. ( $500,000)
6. .S. Dee and Dr R. Cochrane from Pipestone Veterinary Service, A Shah from SAM Nutrition’s, Dr. M. Niederwerder from Kansas State, University, Dr. E. Nelson, C. Jones, and D. Hanson from South Dakota State University collaborate on: Using a demonstration project to validate laboratory-based viral survival in feed: Phase 2. Swine Health Information Center. ($15,000)
7. CL. Miller from USDA and Dr. Y. Sang, Dr B. Lepenies and Dr. D. Fleming from Tennessee State University collaborate on: Validation of A Live-Virus Vaccine Candidate for Efficient Attenuation and Better Protection. USDA, 5030-32000-230-068-R. ($640,000)
8. Niederwerder M and Dr. Hefley T from Kansas State University and Dr G. Cino from Oklahoma State University collaborate on a National Pork Board and State of Kansas National Bio and Agro-defense Facility Fund ($178,808)
9. S. Ramamoorthy and Dr. B. Webb from North Dakota State University and Dr. A. Pillatzki from South Dakota State University, collaborate on: first response vaccines for emergency preparedness. USDA-NIFA. ($337,425)
10. S. Ramamoorthy and Dr. B. Webb from North Dakota State University and Dr. A. Pillatzki from South Dakota State University, collaborate on: first response vaccines for emergency preparedness. NIH-NIAID R21. ($398,749)
11. S. Ramamoorthy and Dr. B. Webb from North Dakota State University and Dr. A. Pillatzki from South Dakota State University, collaborate on: Integrating vaccine safety and efficacy by directed suicidal replication. USDA-NIFA. ($500,000)
12. D. Diel from Cornell University and Dr R. Gourapura form The Ohio State University collaborate on: Novel broadly protective swine influenza vaccine platforms. NIFA (Proposal 2021-06981. ($642,000)
13. H. Vu from University of Nebraska-Lincoln and Dr. P. Gauger from Iowa State University and Dr. H. Ly from University of Minnesota collaborate on: Development of a broadly protective vaccine against swine influenza virus. USDA-NIFA ($500,000)