Brief Summary of Minutes of Annual Meeting

Objective 1. CONTROL OF PRRSV

 1.1. PRRS immunology/vaccinology:

Correlation of protection and neutralizing antibodies (UMN), differences in antibody response based in genetic host background (UMN), immunity related with quasispecies and cross protection between different PRRSV linages (UMN), and genetic effects on PRRSV persistence (BARC). Studies were performed to understand heterologous PRRSV protection (UConn, UNL), immune response against to synthetic vaccines (UNL), antiviral activity by over-expression of IFITM3 and ZMPSTE 24 (SDSU), viral infection interaction with interferon-activated JAK/STAT pathway (UMD), reduction in stress granules (SDSU), and effect of virus expression of interferons (NACD)

 1.2. PRRS epidemiology:

Studies were performed on genome variation in highly pathogenic PRRSV, epidemiological factors associate with PRRSV elimination, role of animal movement networks in PRRS epidemiology, airborne exposure, biosecurity methods for PRRSV inactivate, sampling method and processing fluids for farrowing barns, and spatiotemporal cluster of ORF5 (UMN).

 1.3. PRRS Surveillance and Diagnostics:

Development of the Swine Pathogen Database (https://swinepathogendb.org) (NACD). Phylogenetic analysis of diagnostic PRRSV samples was examined for SDSU, ISU and KSU NACD and evaluation of quasispecies in re-emergent populations (UMN). First detection and characterization in Peru (SDSU)

 Objective 2 Developing effective and efficient approaches for detection, prevention and control of pressing viral diseases of swine of recent emergence

2.1: ASFV

Epidemiological status of African swine fever in in Republic of Armenia (Uconn) and genetic Characterization of ASFV in Asia and Africa (Uconn). Survivability of ASFV in feed, effect in effect in foreign food suppliers, and risk for viral introduction on US (UMN)

2.2: Swine Influenza Virus:

Vaccine research: Subunit vaccines base on Alphavirus vectors (NACD), nanoparticle vaccine (OSU) cross-protective efficacy of intranasal nanovaccine by incorporating CpG-ODN (OSU), hemagglutinin vaccine immunogen against H3 influenza A viruses of swine (UNL), heterologous prime-boost using whole inactivated virus (UMN), effect of sow vaccines in pigs at weaning (UMN), and transmission of live attenuated influenza vaccine to non-vaccinated pigs (UMN), polymer-based vaccine delivery system for broadly protective peptide vaccine for SIV (NDSU)

Epidemiology research: Influenza transmission and persistency (UMN), risk of transmission of Avian influenza to humans (UMN) and zoonotic potential of human derived influenza (UMN). Antigenic evolution of H3N2 influenza A in viruses United States (NACD), human-origin influenza A (H3N2) reassortant viruses in swine in Mexico (UMN). Role of Influenza D in Swine Influenza other agricultural animals (SDSU).

Diagnostic research: Automated classification tool for influenza viruses (NACD), validated novel sampling methods (UMN), developed a GMR biosensor chip (UMN), monoclonal antibodies specific for Influenza D virus (SDSU), primary porcine respiratory epithelial cells to study swine influenza viruses (SDSU).

2.3 Porcine Circovirus:

Evaluation of nucleotide variation that affects PRRSV vaccination and clinical response against PCV2 and PRRSV challenge (BARC), characterization of new linear B cell epitopes of the capsid protein (NDSU)

2.4 Swine Pestiviruses:

2.5: Senecavirus:

Studies were conducted to estimate the Senecavirus A (SVA) seroprevalence in US swine herds (UMN). Characterization of SVA pathogenesis (NADC, SDSU), immune response (SDSU), persistent infection, and clinical response to a potential live attenuated vaccine (SDSU). New diagnostic tools that include Mab specific antibodies for IHC detection of SVA on lesions (SDSU).

2.6:  Sapelovirus:  

2.7: Viruses with potential interest to Xeno-transplantation science:

2.8: Porcine Coronaviruses

Studies were conducted to evaluate PEDV immune repose and infection effect on gilts post farrowing (NADC). Development and characterization of a recombinant porcine delta coronavirus virus (PDCoV) (NADC). Development of rapid response vaccines using PEDV as a model (NDSU)

 Publications/funding sources: (see attached “2019 NC229 Publications”).

Authorization:  Submission by an AES or CES director or administrative advisor through NIMSS constitutes signature authority for this information.

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