Brief Summary of Minutes of Annual Meeting

SHORT-TERM OUTCOMES:

CA: Anaerobic soil disinfestation (ASD) was highly effective against plant-parasitic nematodes if done in a defined protocol at the proper time of year.

CT: Determined that multiple nematode antagonistic crops could be grown in a single season to reduce populations of lesion and root-knot nematodes. Determined that Litchi tomato, Solanum sisymbriifolium, is an effective trap crop that has similar results to soil fumigation.

FL: Data from our field and microplot trials were used to revise University of Florida nematode management recommendations. Stakeholders were educated in proper nematode sampling techniques. They were educated on how to interpret nematode diagnostic reports and to select the best management programs available based on their budget and nematode species complex.

HI: Field experiments verified that a proper biofumigation protocol that involved growing brown mustard cover crops for 5 weeks, followed by flail mowing, soil incorporation and soil tarping of the brown mustard residues with black plastic mulch can suppress population densities of Meloidogyne spp. (M. incognita and M. javanica) and Rotylenchulus reniformis consistently in Hawaii.

Positive relationship between corn yield and soil health (using nematode structure index as an indicator) was observed in a 9-consequtive years of no-till vs tilled field plots, providing more evidence that improving soil health improves crop productivity.

IL: Involvement in efforts sponsored by the Soil Health Institute will contribute to support the development of interpretation frameworks for soil health assessment and using indicators of nematode community structure.

MI: Differences of nematode control was evaluated for new and existing nematicides. Designer made compost and manures were evaluated for nematode control and we found some that are very effective. Evaluation of impact on soils health of these strategies were also evaluated. The crops in which there were trials were corn, soy, sugar beets, potatoes, vegetables (carrots), fruits, ornamentals (daylilies). The information was extended through grower talks (55), videos, webinars, in-service trainings, websites, and extension publications.

MN: Evaluated 43 soybean varieties for SCN resistance and the data have been provided for farmers’ use. Observed long-term crop sequence effect on soil microbial community, plant-parasitic nematode population density, and nematode community; determined the relationship between the soil microbial and nematode communities with crop productivity in the US Midwest cropping production system. The information is useful for developing strategies to enhance soybean and corn productivity.

MS: Chemical nematicides were tested for efficacy and crop tolerance in sweetpotato in Mississippi. No phytotoxicity was observed from any treatment. However, there were also very few meaningful differences in soil sampled nematode counts and sweetpotato yield of any grade. Although research results from the 2017 cropping season were inconclusive, outreach efforts intended to inform producers of the risks associated with a new nematode pest, Meloidogyne enterlobii, resulted in a grower supported quarantine of sweetpotatoes and other restricted articles from states known to have this pests (Florida, North Carolina, and South Carolina) in an effort to protect the $123 million industry.

VT: Field and laboratory experiments reveal mechanisms of poultry-based composts to enhance the survival of enteric pathogens is more than simply supplying large doses of ammonia, increased water holding capacity to the point of anoxia, and contained more bacteria of the phyla WS3, Actinobacteria, Fibrobacteres and Plantocycetes than dairy composts or the control without compost. This affects organic vegetable farmers in Vermont that commonly use these poultry products for economic reasons. Poultry based compost enhanced survival of enteric pathogens in soil more than dairy-based compost. This may shift the choice away from poultry products that are currently used by vegetable farmers for economic reasons, and may affect their acceptance by organic certification programs.

OUTPUTS:

CA: 10 extension presentations and one departmental seminar in 2018.

CT: Two referred articles, 2 chapters, and 1 abstract.

FL: We developed and implemented the NClinic database for nematode diagnostic labs for facilitation of nematode diagnosis and information sharing.

HI: Two short courses related to nematode and soil health management were provided to farmer training programs in Hawaii and course materials are posted online:Four abstracts and presentations related to nematode and soil health management were delivered through Society of Nematologists annual meeting and University of Hawaii, College of Tropical Agriculture and Human Resources (CTAHR) student research symposium: Two extramural grants funded related to NE1640 totaling approximately $100,000 from NRCS and WSARE.

MI: Four oral presentations, five posters, ten extension publications, three workshops, two webinars, two websites, thirteen media or news articles, fifty-five extension/out reach presentations and twenty-five grants obtained.

MN: Two journal articles have been published, two manuscripts have been submitted to journals for review, and drafts of three others have been written. 

NY: Presentation to about 100 growers about root knot and lesion nematodes on potatoes and approximately 40 diagnostic nematode samples processed in 2018.

TN: Three abstracts were given at the Annual SON meeting and three web-based presentations and videos were developed, aimed at educating the general public.

VT: Eight different presentations were given to various grower groups and scientific audiences.

ACTIVITIES

Objective 1. Develop and integrate management tactics for control of plant-parasitic nematodes including biological, cultural (such as rotation or cover crops and plant resistance), and chemical.

CA: greenhouse experiments are conducted to determine the host status of legumes and brassicaceae plant lines towards plant-parasitic nematodes of major damaging potential in nut tree crops, Pratylenchus vulnus and Mesocriconema xenoplax. Field experiments for a winter cover crop period are established.

CT: Experiments were conducted to utilize a series of lesion nematode-suppressive rotation crops in tilled or no-till systems to try to achieve multiple cycles of suppression within a single year. Under good conditions for crop establishment and biofumigation the rotation sequence was successful, but poor conditions for biofumigation resulted in poor nematode management.

We conducted field microplot experiments to evaluate the effects of nematode-susceptible or nematode-resistant plants, Litchi tomato, eastern black nightshade, and a cultivated bare fallow on Globodera tabacum cyst nematode population density changes. Litchi tomato was the most effective at reducing populations and G. tabacum may be useful as a substitute model for the quarantined pathogen Globodera pallida for conducting trap crop experiments with S. sisymbriifolium under field conditions.

FL: We conducted 18 field trials, 5 greenhouse trials, and 2 microplot trials evaluating 15 currently labeled pesticides and 11 numbered active ingredients or experimental formulations on turfgrasses and ornamentals. We completed a 2-year field trial evaluating the effects of turfgrass nematicides on non-target nematodes and other soil invertebrates. We concluded a series of field and microplot experiments evaluating effects of different organic amendments incorporated at planting turf or top-dressed to established turf on nematode population dynamics and turf health.

HI: Three field trials were conducted in Hawaii to determine the best termination method of brassicacea cover crops for soil biofumigation against plant-parasitic nematodes. Results showed that this biofumigation protocol suppressed population densities of both Meloidogyne spp. and Rotylenchulus reniformis consistently.

MA: The following products were trialed for their efficacy against plant parasitic nematodes: Diatomaceous earth, Monterey Nematode Control (saponins from Quillaja saponaria) , Majestene (Burkholderia spp. strain A396), Todal (Abamectin) and Nimitz Pro (Fluensulfone). None of these products reduced the nematode population significantly but the Nimitz Pro treatments had lower populations of nematodes than the untreated control.

MI: Our trials have evaluated tactics for control of plant parasitic nematodes. We have evaluated new and established nematicides, bio-nematicides (biological), compost and manures, crop rotations, plant resistance and cover crops. In carrots and potatoes our trials have included mainly nematicides and compost. One particular compost is effective in lab trials and in some of the field trials. In soybeans we are evaluating rotation with resistant varieties to prevent building of (Soybean Cyst Nematode) SCN resistance, manures, and seed treatments. From greater to less effectiveness in SCN our and others trials: Resistant varieties and rotation of resistant varieties, rotating with non-host, chicken manure, and seed treatments. In sugar beets, we have evaluated nematicides, seed treatments, and trap crops. In corn, we have completed a survey of nematodes in corn field in the state of Michigan. In fruits, we are testing different strategies for prevention of replant problem (apples (cover crops, rootstock, killing old trees with herbicide among others) and cherries (mulch with Dr. Bird)), and in ornamentals we are evaluating nematicides, fumigants, and cultural strategies to control Northern Root Knot nematodes in daylilies. We have gotten excellent results in many of our trials.

 As indicated in previous reports, we continue to integrate the soil food web (SFW) and the fertilizer use efficiency (FUE) models. The SFW model identifies outcomes of soil amendment treatments, and the FUE model identifies the sustainability of the outcomes. Both models are excellent diagnostic tools for translating complex biological and process-based outcomes into practical applications.

MN: In 2018, a total of 43 private and public soybean cultivars were assayed for their resistance to SCN HG Type 7 (race 3) in the greenhouse. Advanced soybean breeding lines were evaluated for their resistance to SCN populations in the greenhouse, and a few of them were tested for yield in fields. Experiment was established at four field locations in Minnesota in 2016 to study the effect of oilseed cover crops pennycress and camelina on the soybean cyst nematode population. The fields were planted with SCN- susceptible and resistant soybeans in 2016 for each site. The cover crops were planted after harvesting soybean in 2016, and harvested before planting corn in 2017. In 2018, SCN population densities were determined at four sampling points.

MS: Trials were conducted in Pontotoc and Starkville, MS to determine the influence of Nimitz (ai. fluensulfone) and Velum Prime (fluopyram) on reniform nematode control and sweetpotato crop tolerance.

NY:  Cornell AgriTech continued to investigate damage threshold levels for root knot nematode (Meloidogyne hapla) and lesion nematode (Pratylenchus penetrans) on potato in NY, and to develop DNA-based methods to quantify nematode populations in soil. In 2018, a commercial field of potato was intensively sampled prior to planting and immediately prior to harvest. At each time, soil samples were obtained from 3 grids, each comprising 100 sample points, and nematodes extracted and counted. Yield and quality of tubers at each location was also assessed. Data will be analyzed to derive relationships between pre-plant numbers of nematodes and (i) pre-harvest nematode numbers, (ii) yield, and (iii) quality of tubers. A method of extracting nematode DNA from 100 g soil samples was successfully developed for mineral soils, and published. A qPCR method has been developed for the selective quantification of Meloidogyne hapla based on the effector gene 16D10. This work has been submitted to a journal.   The extraction method and qPCR is currently being used to extract and quantify DNA from a total of over 500 soil samples collected in 2016, 2017, and 2018 to investigate the relationship between manual techniques of enumerating nematodes and quantification by qPCR.  Ditylenchus dispaci (bloat nematode) continues to be an issue for NY garlic growers and Cornell AgriTech provides a service in testing for Ditylenchus dipsaci (bloat nematode). Ditylenchus dipsaci was detected in garlic bulbs from some farms in 2018, including bulbs intended for seed.

TN: In cooperation with biosystems engineers at the University of Tennessee we (Kimberly Gwinn-UT, Wenqing Zhou-Boragen collaborating) we have developed an automated detector of C. elegans and M. incognita J2 movement in 96-well plates, with data uploaded to a computer. Using this apparatus we are investigating extracts of Chenopodiaceae and proprietary compounds from Boragen, Inc., for their ability to paralyze or kill M. incognita.

Objective 2. Determine the ecological interactions between nematode populations, nematode communities, ecosystems and soil health.

CA: multiple field experiments testing the concept of so-called orchard recycling were collected. In this strategy ground dried tree residues after removal of an old almond orchard are incorporated before replanting to almond. In greenhouse test, these soils are examined for the effects of the wood amendments on plant-parasitic nematodes damaging on almond.

In a second project, anaerobic soil disinfestation is tested for reducing soil infestations with plant-parasitic nematodes to a depth of 5 ft.

HI: Canonical Correspondence Analysis depicted that abundance of bacterivorous nematodes or enrichment index were positively related to efficacy of biofumigation. Field trials in Hawaii using ‘Sod Buster’ oil radish as a cover crop in a 9-year no-till field followed by a corn planting improved soil physical properties (field capacity, soil organic matter, cooler soil temperature) and led to higher abundance of bacterivorous nematode abundance, and indigenous entomopathogenic nematode (Heterorhabditis spp. H1) compared to a conventional tilled bare ground system.

MA: In 1999, Rochester NY golf greens were sampled to see if there was a relationship between fumigation of the greens (5 years previous to the sampling), and the number of Meloidogyne naasi juveniles, and the number of juveniles infected by Pasteuria. Three fumigated greens were compared to 3 non-fumigated greens. The fumigated greens had 7.5 times more juveniles than the non-fumigated greens. Thirty six percent of the juveniles in the fumigated greens were encumbered by Pasteuria. In the non-fumigated greens, 63% were incumbered with Pasteuria. We came to the conclusion that fumigation killed off natural enemies and the root-knot population soared. The population in the non-fumigated greens appeared to be held back by Pasteuria. The same six greens were extensively sampled in 2018, Nineteen years after the first study. The non-fumigated greens had 2.5% more juveniles and only 12% were infected by Pasteuria; a complete turn-around.

MI: In many of our trials we also identify free living nematodes (nematode communities) and other aspects of soil health. We have also tied the nematode populations with other organisms such as bacteria and fungi in our apple replant trial (collaborative effort). Sunnhemp cover crop has resulted in greater amount of beneficial organisms. Several other strategies are showing effective results in soil health. 

MN: Long-term corn-soybean rotation effect on microbial communities associated with the soybean cyst nematode: Field plots of long-term corn-soybean crop sequences were established in 1982 in Minnesota, USA: (i) five-year rotation of each crop such that both crops are in years 1, 2, 3, 4, and 5 of monoculture every year; (ii) annual rotation of each crop with both crops planted each year; (iii) continuous monoculture of each crop. Samples of bulk soil, rhizosphere soil, rhizoplane soil, crop roots, and SCN cysts were collected in 2014-2016 to study crop sequences effect on fungal and bacterial communities associated with SCN with cultural methods as well as metabarcoding DNA sequence analysis. Work accomplished in 2018 include: (1) fungi isolated from cysts were identified with ITS sequence, and the effects of crop sequence, year, season, and replicate on the fungal abundances and diversity were analyzed; (2) fungal DNA extracted from SCN cysts, bulk soil, and rhizosphere soil were sequenced for ITS1 region, and the fungi were identified. The effects of crop sequence, sampling time on the fungal communities were analyzed.

TN: Previous research in a beaver decomposition experiment showed that the top 2 cm of substrate, whether under an animal or from control plots, contained 2-5× the number of nematodes as a comparably sized sample from composited 20-cm cores. Nematode composition in 0-2-cm control samples, including plant parasites, were similar to standard soil cores. The same results are being obtained in an experiment with human donors at the UT Anthropological Research Facility (the “Body Farm”). Both experiments were conducted in forest. If similar results are obtained from agricultural sites, nematode sampling will be simplified without loss of accuracy.

Experiments were conducted to determine the response of nematode food webs to increasing levels of physical disturbance in a forest ecosystem, and another to determine the effects of soil warming on nematode communities. Nematodes belong to higher trophic groups and higher CP classes were more impacted by increasing levels of physical disturbance than lower groups. Soil warming gradually decreased the richness and the abundance of higher trophic group and higher CP class nematodes, but led to increasing abundance of lower trophic group and lower CP class nematodes.Soil warming may enhance microbial activity, resulting in increased abundance of lower trophic groups.

Objective 3. Outreach and communication - Compile and present/publish guidance on nematode management and management effects on soil health for different crops under different conditions.

CA: The PI gave 10 extension presentations and one invited seminar on the management of plant-parasitic nematodes including chemical and biorational methods. He also taught classes to K-12 students.

CT: Chaired a contributed paper session on nematode management at the SON meeting in New Mexico and presented ‘Rotation crops for management of Pratylenchus penetrans in Connecticut’ (July 24, 100 attendees); spoke to growers about ‘Identifying, understanding and managing nematode diseases in vegetables’ at the CPS Vegetable Growers meeting held in Glastonbury CT (March 7, 65 persons); spoke about ‘Identifying, understanding and managing nematode diseases in potatoes’ (January 10, 50 people) and ‘Identifying, understanding and managing nematode diseases in vegetables’ (January 11, 75 people) at the Long Island Agricultural Forum held in Riverhead NY; and presented research results during the potato cyst nematode multi-agency research call (November 14, 20 participants). Dr. LaMondia conducted 125 nematode diagnostic samples and conducted testing as an APHIS certified pinewood nematode export testing facility.

FL: Nematode management training was presented to turfgrass stakeholder groups at 23 seminars, 3 field days, and 2 workshops to a combined audience of 1,842 in Florida, Michigan, and Rhode Island. Turfgrass nematode management articles were written in the two most widely read and highest quality turfgrass trade magazines, the Green Section Record and Golf Course Management. Three University of Florida Cooperative Extension documents were updated and revised. Diagnosis was provided for 6205 samples submitted to the University of Florida Nematode Assay Lab from around the US.

HI: Six workshops/field days were presented statewide in Hawaii to educate vegetable crop farmers, new farmers, legislators and other agriculture professionals about nematode and soil health management on food crop production systems.

IL: The Soil Health Institute has been working to establish measurements and standards for the assessment of soil health across the country. Ugarte attended the Soil Health Institute conference held in Albuquerque, New Mexico from August 1 to August 3, 2018 and presented a poster that outlined best practices to build metadata standards that could facilitate the use and interpretation of soil health indicators.

MA and RI: A presentation, January 4th on “Introduction to Nematodes” was given at the Michigan State Turfgrass Conference to about 250 golf course superintendents. January 24^th a presentation on “Management of Nematodes in Turfgrasses”, sponsored by Advanced Turf Solutions, about 75 in attendance. March 6^th, “The Biology of Nematodes” and the New England Regional Turfgrass Conference. The UMass Extension Nematology Lab processed about 200 soil samples, the majority of which came from golf courses.  The URI Turfgrass Diagnostic Laboratory processed approximately 150 soil samples for nematode analysis.  Each sample offered a teaching moment for the superintendent who received results, a fact sheet about nematodes in turfgrasses and written recommendations.

MI: Our outreach and communications efforts have been extensive. For crops that were in our trials (corn, soy, sugar beets, potatoes, vegetables (carrots), fruits, ornamentals (daylilies)). The information has been extended through grower talks (55), videos, webinars, in-service trainings, websites, and extension publications. Our website includes videos and extension efforts and images of most nematodes identified. Growers have had information and reports given to them personally and in grower talks. Relationship with extension agents and commodity groups has been essential in these efforts. These outreach efforts have included information on plant parasitic nematode control and also increase of soil health.

MS: A presentation entitled “Guava Root-Knot Nematode Updates” was given at the annual Mississippi State University Sweetpotato Field Day August 30, 2018 at the Pontotoc Ridge-Flatwoods Branch Experiment Station, Pontotoc, MS to more than 80 attendees. An outdated list of recommended nematode tolerant sweetpotato varieties was updated at the Mississippi State University Plant Diagnostic Lab to ensure recommendations made include contemporary cultivars currently used in commercial production environments in Mississippi.

TN: A paper by G. Phillips et al., now in press with American Biology Teacher, described the diversity of nematodes in the intestine of North American millipedes and describes the techniques for collecting them from the millipede. These nematodes are an ideal approach for students to study the anatomy of nematodes similar to typical rhabditids. The advantages are that these nematodes are usually much larger, the organs are more easily seen, and they can be obtained in a few minutes by dissecting a suitable millipede. Related videos were made and posted to YouTube.

ACOMPLISHED MILESTONES (2018):

 CA: Continue cover crop experiments. Currently one in open field planting, and three in orchard experiments are ongoing.

 CT: Tested putative M. hapla-resistant pepper against CT isolates of the nematode.Continued and adjusted cover- and rotation-crop experimental designs based on previous results

HI: Evaluate new nematicidal products for efficacy – PI in Hawaii evaluate fluopyram against plant-parasitic nematodes on vegetable cropping systems in Hawaii

Investigate the relationship between the microbial community, plant-parasitic nematodes, soil health, and crop productivity – We present Canonical Multivariate analysis in the biofumigation trials and long-term no-till cover cropping trials to examine relationships between soil health and crop productivity.

Conduct grower education, annual short course and webinar (6 client presentations, 2 short courses related to nematode and soil health management were presented to farmers in Hawaii).

MN: Continue cover- and rotation-crop experiments. Investigate the relationship between the microbial community, plant-parasitic nematodes, soil health, and crop productivity

Multiple states: New nematicidal products were tested for efficacy and two collaborative, gay-long turfgrass nematology seminars were given (Michigan and Providence, RI)

Bird, G., G. S. Abawi and J. A. LaMondia. 2018. Plant Parasitic Nematodes of New York, New Jersey and Pennsylvania. Chapter 3, Plant Parasitic Nematodes in Sustainable Agriculture in North America” edited by S. A. Subbotin and J. J. Chitambar, Springer. November 2018

Carta, L. and Wick, R. L. Bursaphelenchus antoniae from Pinus strobus in the U.S. 2017. The Journal of Nematology (in review).

Cheng, Z., H. Melakeberhan, S. Mennan, and P.S. Grewal (2018). Relationship between soybean cyst nematode Heterodera glycines and soil nematode community under long-term tillage and crop rotation. Nematropica 48, 101-115.

Cho, A., Quintanilla, M., McDonald, T., Kawabata, A., and Nakamoto, S. 2017. ‘Sharwil’ Avocado Identification. University of Hawaii CTAHR Extension Publication F_N-50. http://www.ctahr.hawaii.edu/oc/freepubs/pdf/F_N-50.pdf

Crow, W. T. 2017. The Killer: Belonolaimus longicaudatus. On the Turf Winter:23-26.

Crow, W. T. 2018. Nematode management on athletic fields. Florida Turf Digest 35:8-16.

Dandurand L. M., I. A. Zasada, and J. A. LaMondia 2018. Effect of the trap crop, Solanum sisymbriifolium, on Globodera pallida, Globodera tabacum, and Globodera ellingtonae Journal of Nematology in press.

Gorny, A., Hay, F.S., Wang, X., & Pethybridge, S.J. 2018. Isolation of nematode DNA from 100 g of soil using Fe₃O₄ super paramagnetic nanoparticles. Nematology 20, 271-283.

Grabau, Z. J., Vetsch, J. A., and Chen, S. Y. 2018. Swine Manure, Nematicides, and Long-Term Tillage Change Soil Ecology in Corn and Soybean Production. Agronomy Journal 110:2288-2301.

Gu, M., and W. T. Crow. 2018. Abamectin, thiophanate-methyl, and iprodione for management of sting nematode on golf turf. Nematropica 48:38-44.

Habteweld, A. W., Brainard, D. C., Kravchenko, A. N., Grewal, P. S. & Melakeberhan, H. (2018). Effects of plant and animal waste-based compost amendments on soil food web, soil properties, and yield and quality of fresh market and processing carrot cultivars. Nematology 20, 147-168. DOI: 10.1163/15685411-00003130

Hu, W. M., Strom, N., Haarith, D., Chen, S. Y., and Bushley, K. E. 2018. Mycobiome of cysts of the soybean cyst nematode under long term crop rotation. Frontiers in Microbiology 9:DOI: 10.3389/fmicb.2018.00386.

Huang, D., G. Yan, N. Gudmestad, J ,Whitworth, K. Frost, C. Brown, W. Ye, P. Agudelo, W. Crow. 2017. Molecular characterization and identification of stubby root nematode species from multiple states in the United States. Plant Disease 102:2101-2111.

Heve, W. K., F. E. El-Borai, E. G. Johnson, D. Carrillo, W. T. Crow, and L. W. Duncan. 2018. Responses of Anastrepha suspense, Diachasmimorpha longicaudata, and sensitivity of guava production to Heterorhabditis bacteriophora in fruit fly integrated pest management. Journal of Nematology 50:261-272.

Jones, W. B., and W. T. Crow. 2018. Nematodes, turfgrass, and organic amendments. Clippings Winter:6.

LaMondia, J. A., R. L. Wick and N. A. Mitkowski. 2018. Plant Parasitic Nematodes of New England – Connecticut, Massachusetts and Rhode Island. Chapter 1, Pp.. Plant Parasitic Nematodes in Sustainable Agriculture in North America” edited by S. A. Subbotin and J. J. Chitambar, Springer. November 2018

LaMondia, J. A. and L. M. Dandurand. 2017. Effects of resistant or susceptible tobacco (Nicotiana tabacum), eastern black nightshade (Solanum ptychanthum), and litchi tomato (Solanum sisymbriifolium) on reproduction of the tobacco cyst nematode Globodera tabacum. Journal of Nematology 49:509-510.

Lindberg, H., Quintanilla, M., and Poley, K. 2018. Nematodes in ornamental plant production: Good or bad? MSU Extension. http://www.canr.msu.edu/news/nematodes-in-ornamental-plant-production

Lindberg, H., Quintanilla, M., Horling, K., and Poley, K. 2018. Combating root-knot nematodes in daylilies: Experimental results. MSU Extension. http://www.canr.msu.edu/news/combating-root-knot-nematodes-in-daylilies

Melakeberhan, H., Maung, Z.T.Z., Lee, C-L., Poindexter, S., Stewart, J. (2018). Soil type-driven variable effects on cover- and rotation-crops, nematodes and soil food web in sugar beet fields reveal a roadmap for developing healthy soils. European Journal of Soil Biology 85, 53-63.

Mishra, S., K.-H. Wang, B. S. Sipes, M. Tian. 2018. Induction of host-plant resistance in cucumber by vermicompost tea against root-knot nematode. Nematropica 48: (in press).

Monteiro, T. S. A, J. A. Brito, S. J. S. Vau, W. Yuan J. A. LaMondia, L. G. Freitas, and D. W. Dickson^. 2017. First report of matricidal hatching in Meloidogyne hapla. Nematoda 4:e092017. http://dxdoi.org/10.4322/nematoda.00917.

Neher, D.A., Weicht, T.R. 2018. A plate competition assay as a quick preliminary assessment of disease suppression. Journal of Visualized Experiments e58767, doi: 10.3791/58767. https://www.jove.com/video/58767?status=a60773k

Quintanilla, M. 2017. Soil Acoustics. In: A. Farina and S.H. Gage (Eds.). Ecoacoustics: The ecological role of sounds. Wiley Press.

Quintanilla, M. 2018. The New Soybean Cyst Nematode Coalition. Michigan Soybean News. Fall Issue, p. 21.   http://www.misoy.org/michigan-soybean-news/

Quintanilla, M., Shoemaker, J., Bird, G., Tenney, A., Warner, F., and Poley, K. 2018. Soybean Cyst Nematode Resistance Management. MSU Extension. http://www.canr.msu.edu/news/soybean-cyst-nematode-resistance-management

Quintanilla, M., Shoemaker, J., Bird, G., Tenney, A., Warner, F., and Poley, K. 2018. Soybean Cyst Nematode Resistance Management Workshop held June 20, 2018. MSU Extension. http://www.canr.msu.edu/news/soybean-cyst-nematode-resistance-management-workshop-held-june-20-2018

Quintanilla, M., Warner, F., 2018. Nematode Management. In: J.C. Wise, L.J. Gut, J. Wilson, M. Grieshop, M. Whalon, D. Mota-Sanchez, M. Quintanilla, R. lsaacs, A.M.C. Schilder, G.W. Sundin, B. Zandstra, R. Beaudry, G. Lang, L. Jess, D. Elsner, W. Shane, M. Longstroth, C. Garcia-Salazar, and D. Brown-Rytlewski. Fruit Management Guide. Michigan State University Extension Bulletin E-154, pp. 311-314. Michigan Potato Research Report 2017: http://www.canr.msu.edu/potatooutreach/research/michigan-potato-research-report

Steel, H., Moens, T., Vandecasteele, B., Hendrickx, F., De Neve, S., Neher, D. A., and Bert, W. 2018. Factors influencing the nematode community during composting and nematode-based criteria for compost maturity. Ecological Indicators 85: 409-421. doi 10.1016/j.ecolind.2017.10.039.

Waisen, P. and Wang, K.-H. 2018. Trap cropping and biofumigation for plant-parasitic nematode management. HānaiʻAi Newsletter March, April, May 2018. https://gms.ctahr.hawaii.edu/gs/handler/getmedia.ashx?moid=29943&dt=3&g=12 (extension publication)

Waldo, B. D., and W. T. Crow. 2018. Nematicides and soil health. Clippings Winter:6-7.

You, X., K.-H. Wang, S. Ching, and M. Tojo. 2018. Effects of vermicompost water extract prepared from bamboo and kudzu against Meloidogyne incognita and Rotylenchulus reniformis. Journal of Nematology 50: (in press).