• Basic Information
• Additional Info

Basic Information

• Project No. and Title: WERA89 : Potato Virus and Virus-Like Disease Management
• Period Covered: 10/01/2016 to 09/30/2017
• Date of Report: 10/09/2017
• Annual Meeting Dates: 03/08/2017 to 03/09/2017

Participants

Andrew Houser, Nora Olsen, Lynn Woodhall, Carrie Wohleb, Susie Siemsen, Alice Pilgeram, Phil Townsend, Mary Kreitenger, Amy Charkowski, Ana Cristina Fuladosa, Nina Zidak, Dave Douches, Paul Bethke, Kent Sather, Alan Westra, Johnathan Whitworth, Guiping Yan, Rich Novy, Alex Karasev, Chris Benedict, Kasia Duellman Kinzer, Pablo Guzman, Mark Pavek, Russ Groves, Stewart Gray, Neil Gudmestad, Kylie Swisher, Jim Dwyer, Joe Kuhl, Silvia Rondon, Andrei Alyokhin, Chuck Brown, Chris McIntosh, Keith Schuetz, Raquel Salati, Mathuresh Singh, Andrew Nicholaus from Simplot, Debbie Inglis, Darrin Hall, Washington DeSilva, Erin Weber, Hannu Pappu, John Mizicko from Eurofins BioDiagnostics, Carolyn Keller, Teresa Almeida, Kenneth Frost, Nicole Hostert, Anna Jespersen, Mark McGuire, Alex McCandlewire, James Harris

Accomplishments

Impacts

1. Use of the potato SNP arrays has allowed us to create high quality potato populations to conduct genetic mapping of virus resistance traits. The high density of the genetic markers that are generated will allow for mapping in tetraploid populations by the breeders. Seed tubers are generated of these populations so that PMTV and TRV can be phenotyped.
2. Aphid capture data have been compiled from the North Central Regional, Aphid Suction Trap Network (http://traps.ncipmc.org/) from a span of 10 years (2005-2015) and 45 locations comprising over 180 species of aphids and nearly 1M individual captures in the upper Midwestern US. Additional aphid trapping data from various potato producing states are being identified to species and are planned for validation in the current models. These trap data will be standardized against cumulative growing degree-days (base50) using random effects models, and these will be used to predict the dispersal phenology of unique aphid species in different states.
3. Sixty potato cultivars representing every market class were tested for their sensitivity to PMTV- and TRV-induced tuber necrosis in field trials conducted in 2015 and 2016. Expression of tuber necrosis by each virus was variable among cultivars with a number of them identified as being insensitive to the tuber necrosis phase caused by each virus. The commercial potato industry has cultivars available within each market class that can be used to escape economic loss by utilizing potato varieties that do not express tuber necrosis.
4. 1. Shallow, suberized canoe-shaped cracks on tubers of fresh-market potato varieties can be a symptom of Potato virus Y, especially if the infections arise from seedborne virus. 2. Over three trials at WSU Mount Vernon NWREC (field and greenhouse), ‘All Blue’, ‘Chieftain’, ‘French Fingerling’, ‘Russet Burbank’, and ‘Yukon Gold’ were susceptible to PVY strains O, NTN, and N-Wi, exhibited strong mosaic symptoms, and varying levels of cracked tubers. 3. Potato growers need to employ season-long PVY management when planting these varieties, and avoid purchasing seed tubers from lots exhibiting the cracking symptom.
5. This past year work on a beta-test version of the PVY seed calculator was completed. The calculator represents a “dashboard” style reference for the economic impact of PVY. By selecting an estimated infection level in seed, the user can obtain a mean and 95% confidence interval on the forecasted economic damaged caused by the virus.
6. It has been demonstrated that mechanical PVY transmission (not vectored by aphids) can readily occur in the field due to farming operations. Inoculum tubers known to be infected with PVY of three strains (PVYO, PVYN:O, PVYNTN) were planted in a tractor-trackway row and a control row away from tractor traffic. The virus spread was 3-5 times higher in tractor row than non-tractor row with PVYNTN spreading far more than the other strains (ca. 70% of new infections).
7. Documenting PVY symptoms of potato varieties in the greenhouse and field has shown that recombinant strains have mild symptoms, making visual inspections difficult. Results are being shared with growers and the industry. A PVY demonstration trial was done in Washington in 2016 and additional trials will be done in WA, WI, and ME in 2018.

Publications