Brief Summary of Minutes of Annual Meeting

OBJECTIVE 1: Characterization of host mechanisms associated with mastitis susceptibility and resistance.

Researchers at the University of Georgia observed that dietary supplementation with the immunostimulant OmniGen^® (OG) resulted in less mastitis. Scientists at the University of Idaho demonstrated how dietary changes in vitamin A and protein intake affect inflammatory status.  Researchers at the University of Maryland continue to study the effect of nutrient supply on the immune response and the cross-talk between mammary the liver tissue during mastitis (Oregon State University). Scientists at Michigan State University observed that poly-unsaturated fatty acids attenuate inflammatory responses. Researchers at Rutgers University characterized the mechanisms by which Staphylococcus aureus (S. aureus) builds and maintains inorganic cofactors called iron-sulfur (FeS) clusters. Scientists at Cornell University continue to examine the antibiotic susceptibility of streptococci-like bacteria. At the University of Vermont, scientists observed that the severity of host response to Escherichia coli (E. coli) was quite variable and giving minimal encouragement for the ranking technique on the basis of high (6 cows) or low (6 cows) expression of the TLR4 gene in ear notch samples. Further use of additional ranking parameters will be required to develop a more accurate test to predict a cow’s response to E. coli mastitis. As part of research completed under NIFA funding at Virginia Polytechnic Institute and State University (Virginia Tech), the scientists are currently evaluating the use of lymphocytes as a target for vaccine development. At Utah State University, scientists identified a number of genetic variations (SNPs) that were associated with resistance or susceptibility to bovine mastitis. The primary objectives for researchers at Washington State University to determine ability of isolated neutrophils to phagocytized and kill different strains of opsonized Mycobacterium bovis (M. bovis).  Scientists at the University of Wisconsin observed an association between teat apex diameter with occurrence of both clinical and subclinical mastitis and confirmed an association between occurrence of severe hyperkeratosis and increased somatic cell count (SCC). At the University of Montréal, Canada, scientists are currently investigating cow characteristics associated with recurrence of clinical mastitis. These studies described above aid in providing host mechanisms associated with bovine mastitis susceptibility and resistance that will help formulate new management strategies that will reduce the severity and duration of bovine mastitis.

OBJECTIVE 2: Characterization and manipulation of virulence factors of mastitis pathogens for enhancing host defense.

Data collected from scientists at the University of Georgia suggest that a significant proportion of S. aureus were diagnosed as coagulase- negative staph species and that additional testing should be performed to correctly identify such isolates. Researchers at Louisiana State University are currently investigating mastitis-causing pathogens for dairy cattle and goats regarding their antimicrobial susceptibilities for resistance to antimicrobials. Scientists at the University of Maine are currently conducting in vitro trials of alternative treatments, including evaluation of aromatic compounds and the effects of bedding, for prototheca mastitis in cattle. Work continues at the University of Missouri regarding the relationship between body site colonization and intramammary infection with coagulase negative staphylococci in dairy heifers; as well as evaluating the epidemiology of coagulase negative staphylococcal (CNS) mastitis in dairy goats and the influence of CNS on milk SCC and milk yield in dairy goats. At Cornell University, data suggest that certain strains of S. aureus are better adapted to live in hostile environments. Investigators that Pennsylvania State University have identified a small cell variant that may play a role in the persistence of S. aureus infections. Research scientist at Rutgers University discovered that the application of nitrate to anaerobic cultures of S. aureus can alter virulence factor expression and biofilm formation.  Researchers at the University of Vermont developed a multilocus sequence typing scheme for S. chromogenes and researchers have identified S. aureus strain types with increased biofilm formation capacity under different growth conditions, and with increased antimicrobial resistance phenotypes. Scientists at Virginia Tech identified that lymphocyte number and memory response was modified in live versus modified S. aureus stimulated lymphocytes. International scientists at the University of Montréal are currently investigating the impact of pathogen’s characteristics such as virulence genes, relative biofilm production, antimicrobial resistance phenotypic profiles on clinical mastitis recurrence in characterizing Klebsiella spp. isolates from bovine mammary gland infections. The research studies above will provide further knowledge regarding the characterization and manipulation of virulence factors of mastitis pathogens for enhancing host defense.

OBJECTIVE 3: Assessment and application of new technologies that advance mastitis control, milk quality and dairy food safety.

Researchers at the University of Georgia discovered cure rates were improved in quarters treatment with dry cow therapy (100%), teat seal (85.7%), or dry cow therapy plus teat seal (96.1%) infected prepartum when compared to untreated controls (55.2%) During intramammary challenge with S. aureus or CNS. The University of Kentucky research has focused on (1) the application of new technologies for mastitis detection, (2) management of compost bedded pack barns for maximum milk quality, and (3) field surveys of Southeast dairy farms. At Louisiana State University, novel food grade products are being evaluated using the modified AOAC test and the excised teat model to determine their suitability for teat dips and disinfectants. At the University of Maine, support was requested to expand quality assessment and control for producers of raw milk. Researchers at the University of Maryland continue to examine the efficacies of alternative therapies for treatment of bovine mastitis for the reduction of antibiotic use on-farm. Scientists at the University of Maryland continue to collect data from dairy farms regarding their transition from conventional to robotic/automatic milking systems and how this may impact mastitis. As part of a multistate USDA-NIFA funded project, scientists at Michigan State University developed an on-farm evaluation system for milk quality and reduction of antibiotic use.  The evaluation system (Quality Milk Alliance) assesses traditional practices related to mastitis control as well as a novel integration with social and communication barriers on the part of dairy producers and employees. Scientists at Michigan State University are developing novel applications for VaDia vacuum analysis as a tool to determine milking efficiency in dairy herds.  These standards will be used to evaluate milking protocols and equipment performance and have the potential to provide an on-farm education platform for producers and/or employees regarding milking protocols. Researchers at the University of Minnesota evaluated the use of an Automated Milk Leukocyte Differential (MLD) Test and the California Mastitis Test for detecting intramammary infection in early lactation (EL) and late lactation (LL) quarters. Overall the results suggest that either test may have greater utility in late lactation. Researchers at the University of Minnesota also observed that intermittent cessassion of lactation decreased milk yield and tended to improve cure of bacterial infections in cows. Currently, the scientists are 1) comparing frequent stripping, a topical udder cream and a negative control as treatment for clinical mastitis in dairy cows; 2)  developing and evaluating extraction methods to improve the MALDI-ToF method's ability to detect M. bovis as well as individual species of Bacillus and Paenibacillus of importance to udder health, food safety and food quality; 3) completing a field validation study using 2,500 bacterial isolates were a subset of samples are derived from station members of NE-1048; 4) evaluating the diagnostic test characteristics of a rapid on-farm culture system to identify intramammary infection in individual quarters at dry off, for the purpose of applying selective dry cow therapy; and 5) completing a pilot study to evaluate the effect of applying a culture-guided selective dry cow therapy program, at the quarter level, on measures of quarter health and antibiotic use. Based on analysis of whole genome sequences of Staphylococcus hyicus, Staphylococcus  agnetis, and Staphylococcus chromogenes, scientists at the University of Missouri will generate a multiplex PCR assay that utilizes primers for the aroD gene and nuc gene was developed to differentiate S. hyicus from S. agnetis and S. aureus.  At Cornell University, scientists developed and validated a multitarget PCR assay that can in the same day confirm or reject a presumptive positive mycoplasma culture found upon bacteriological testing of clinical specimens, further discriminate between Acholeplasma and Mycoplasma, and, when coupled with sequence analysis, identify various strains of M. bovis. Incorporation of the validated molecular diagnostic assay into routine bacteriological screening as a supportive confirmation and identification tool will lead to an improved assessment of Mycoplasma and Acholeplasma prevalence data, which will facilitate increased knowledge about the role of these mycoplasma in mastitis. Researchers at Cornell University also developed a mathematical optimization model that maximizes farm profit subject to SCC bulk tank thresholds. Data generated at Oregon State indicated that 2,4-thiazolidinedione or TZD treatment improved the liver response to mastitis, prevented the decrease of milk fat synthesis after mastitis induction, and improved the innate immune system. Scientists at Pennsylvania State University are currently examining the correlation of VaDia® recordings and Lactocorder® milk flow curves was undertaken and are also assessing the level of intra-operator and inter-operator agreement of VaDia® vacuum analyses by multiple VaDia® users. In collaboration with scientists at Michigan State University, they will assess records and on-farm evaluation systems and report/intervention tool on the rate of mastitis, use of antibiotics and milk quality. Researchers at Rutgers University characterized a new molecule that inhibits FeS cluster assembly in S. aureus.  This molecule, or a similar molecule, could be used to treat or prevent S. aureus infections including mastitis. Scientists at Utah State University are comparing (blind comparison) matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) bacteriological results from bovine milk samples to conventional culture results and those from 16S rRNA genomic identification.  The 16SrRNA analyses are in progress. In addition, data generated at Utah State University suggested that a casein hydrolysate intramammary infusion for cessation of lactation can be an alternative method of drying off one mastitic quarter with a good prospect for return of that quarter’s production following the next calving. Researchers at the University of Vermont continue to work in the area of disease detection with the use of daily milk component and animal activity monitoring and feeding behavior in calves.  The results suggested the device can be used to measure step activity, lying time, and lying bouts in unweaned dairy calves housed in groups.  A subsequent study examined changes in activity and feeding behavior of calves around the time of pneumonia.  Results of this study showed marked changes in both activity and feeding behavior prior to and during pneumonia.  A third study is currently underway examining the effects of meloxicam given either before calving or immediately after calving compared to a negative control on animal activity, milk components and milk yield.  A second objective of this study is to examine the effects of pre-calving administration of meloxicam on calf activity and health. Scientists at Washington State University determined how mycoplasma mastitis pathogens grow when incubated at atmospheric concentrations commonly used for culture of Mycoplasma sp. by veterinary diagnosticians. Improved culture of Mycoplasma sp. in the laboratory will increase sensitivity of detection; more accurate detection of infected cows can improve control of an outbreak of mycoplasma mastitis. At the University of Wisconsin, researchers are working to reduce unnecessary use of antibiotics to advance milk quality and ensure dairy food safety by improving treatment recommendations for non-severe culture negative and Gram-negative clinical mastitis occurring in dairy cows. To meet this aim, the researchers have been enrolling cows in 2 negatively controlled clinical trials to evaluate bacteriological cure, clinical outcomes, milk yield, and trends in quarter-level SCC. Scientists at the University of Saskatchewan recently Completed a study on a novel treatment for Staphylococcus aureus mastitis, a study on the effect of different CNS species on udder health and a study on the epidemiology of CNS mastitis on dairy farms. Researchers at the University of Montréal recently described the cost of mastitis in Canada, are currently investigating selective quarter dry treatment for prevention and treatment of intramammary infections during the dry period, identifying sampling strategies for controlling misclassification bias in longitudinal udder health cohort studies, investigating impediment to adoption of mastitis control practices on dairies, examining the impact of recycled manure bedding on udder health and milk microbiota and identifying alternative treatments for prevention and treatment of intramammary infections at dry-off. The studies conducted above will provide further assessment and application of new technologies that advance mastitis control, milk quality and dairy food safety.

WORK PLANNED FOR THE COMING YEAR, LISTED BY OBJECTIVE:

OBJECTIVE 1: Characterization of host mechanisms associated with mastitis susceptibility and resistance.

The University of Georgia: Continue to evaluate the positive effect of dietary supplementation with OmniGen^® and the basis for promoting udder heath by focusing on L-selectin expression on blood leukocytes, phagocytic ability of leukocytes, cytokine expression, and production of reactive oxygen species (ROS).

The University of Idaho: Researchers plan to study the effects of elevated lipomobilization on innate immunity of the mammary glands and metabolism in periparturient dairy cows

The University of Maryland: continue to work on projects described above.

Michigan State University: Continuation of projects stated above.

Rutgers University (New Jersey): We are planning on further examining the antimicrobial effects of copper on S. aureus.  We will examine how S. aureus detoxifies copper and how copper kills S. aureus.

Utah State University: Continue GWAS analyses including identifying Holstein genes and if known, describing their functions.  Evaluate whether there is biological logic to associations with mastitis resistance or susceptibility as observed in the cows, if gene functions are identified.

The University of Vermont:  The Kerr lab will explore the genetic and epigenetic basis for differential expression of the TLR4 gene, and other gene in the TLR4 response pathway.

Virginia Tech: We have completed challenge studies and are working on compilation of data for publication.

OBJECTIVE 2: Characterization and manipulation of virulence factors of mastitis pathogens for enhancing host defense.

The University of Georgia: Continue to assess all non-hemolytic staphylococcal isolates from heifers and cows that are presumptively identified as coagulase-negative staphylococci (CNS) to determine which isolates are actually S. aureus based on coagulase production, fermentation of mannitol, and the API staph test system, to demonstrate that a significant proportion of S. aureus are misdiagnosed as CNS.

Louisiana State University: Evaluation of antimicrobial susceptibility of mastitis pathogens to various antibiotics will continue. Identification of mastitis pathogens from clinical milk samples will continue. Identification of mastitis pathogens from dairy goats will continue.

The University of Maine: Characterization and manipulation of virulence factors of mastitis pathogens for enhancing host defense.Re focus investigations based on current regional mastitis pathogens, informed by our service laboratory work.

The University of Missouri: Work on staphylococcal mastitis in dairy goats will continue.  Dairy heifer projects will be completed and the results published. 

Pennsylvania State University: Analysis of the small cell variant of S. aureus.

Rutgers University (New Jersey): Scientists will continue to examine the mechanisms of S. aureus biofilm formation.  We are interested in the effects of an anoxic growth on biofilm formation and determining if we can disperse fermentative biofilms by introducing a terminal electron acceptor.

The University of Vermont: The Barlow lab will quantify potential associations between S. chromogenes strain types and intramammary infection phenotypes. We will use whole genome sequencing to characterize  S. aureus and S. chromogenes strains with different in vitro phenotypes.

OBJECTIVE 3: Assessment and application of new technologies that advance mastitis control, milk quality and dairy food safety.

The University of Georgia: Continue to research the infusion of nonlactating cow therapy, teat sealant, and the combination into mammary quarters of bred heifers with focus on why teat sealant demonstrated a 85.7% cure rate against S. aureus and CNS.

Louisiana State University: Evaluation of novel products for application as teat dips and antimicrobials will continue.

The University of Maine: Assessment and application of new technologies that advance mastitis control, milk quality and dairy food safety.Investigate a cow-side screening methodology for farms with a history of prototheca mastitis.Continue quality assurance and control efforts to reduce food safety risks posed by mastitis pathogens.Continue investigations of bedding-based environmental control of mastitis pathogens.Continue investigation into pathogenesis and control of Corynebacterium pseudotuberculosis in sheep and goats for dairy and general use.

The University of Maryland: continue to work on projects described above.

Michigan State University: Continuation of projects stated above.

The University of Minnesota: We will complete the animal and laboratory work, data analysis, and reporting for the aforementioned ‘in progress’ projects  listed above.

The University of Missouri: Novel approaches to therapy, e.g. antimicrobial peptides, will be investigated.

Cornell University (New York): Further development of a mathematical optimization model that maximizes farm profit subject to SCC bulk tank thresholds.

Oregon State University: We plan to perform an experiment to assess the role of selenium on the transcriptome of liver and mammary macrophages in early lactating dairy cows

Pennsylvania State University: Continued work on the project with Michigan State University. Complete the investigation into the agreement of VaDia® vacuum analyses. -  Investigate the use of Vadia® data and parlor software data to predict milking performance and mastitis risk at the cow-level.

Utah State University: Study improved casein hydrolyzate protein purity in formulating the intramammary infusion solution.  Investigate the mechanism(s) of how the casein product promotes single mammary quarter involution with minimal clinical severity and maximal return to milk production of the quarter following the next calving.

Virginia Tech: We will continue our on-going studies looking at NSAID administration around calving, and we will begin to examine the ability to detect mastitis using changes in milk components and yield, as well as animal activity.

The University of Wisconsin: Researchers will conclude enrollment and data collection in both clinical trials, perform analysis and submit papers. 

Peer-Reviewed Literature

University of Georgia:

Berry, E. A., J. E. Hillerton, H. Hogeveen, J. S. Hogan, S. C. Nickerson*, S. P. Oliver, G. M. Pighetti, P. Rapnicki, Y. H. Schukken, and K. L. Smith. 2016. Book: Current Concepts of Bovine Mastitis, 5th Edition. National Mastitis Council, New Prague, MN.

Enger, B., R.R. White, S.C. Nickerson, and L.K. Fox^. 2016. Meta-analysis of factors influencing teat dip efficacy trial results in reducing the development of new intramammary infections. J. Dairy Sci. 05 Oct. 2016. http://dx.doi.org/10.3168/jds.2016-11359.

Nickerson, S.C. 2015.  Managing Mastitis through Proper Dry-Off Procedures EXTENSION BULLETIN (B 1447) http://extension.uga.edu/publications/detail.cfm?number=B1447.

Nickerson, S.C. 2015.  Control of Mastitis and Milk Quality in Dairy Goats through Immunization BULLETIN (B 1446) http://extension.uga.edu/publications/detail.cfm?number=B1446.

Nickerson, S.C. and L.M. Sordillo^. 2016. Book Chapter: Modulation of the bovine mammary gland. In Large Dairy Herd Management. 3rd ed. D. K. Beede, ed. American Dairy Science Association, Champaign, IL. http://dx.doi.org/10.3168/ldhm.

University of Kentucky:

Liang, D., L.M. Arnold, C.J. Stowe, R.J. Harmon, and J.M. Bewley. 2016. Estimating U.S. clinical dairy disease costs with a stochastic simulation model. J. Dairy Sci. (Accepted)

Klefot, J. M., J. L. Murphy, K. D. Donohue, B. F. O’Hara, M. E. Lhamon, and J. M. Bewley. 2016. Development of a noninvasive system for monitoring dairy cattle sleep. Journal of

Eckelkamp, E. A., J. L. Taraba, K. A. Akers, R. J. Harmon, and J.M. Bewley. 2016. Sand bedded freestall and compost bedded pack effects on cow hygiene, locomotion, and mastitis indicators. Livest. Sci. 190:48-57.

Eckelkamp, E. A., J. L. Taraba, K. A. Akers, R. J. Harmon, and J.M. Bewley. 2016. Understanding compost bedded pack barns: Interactions among environmental factors, bedding characteristics, and udder health. Livest. Sci. 190:35-42.

Lowe, J.L., A.E. Stone, K.A. Akers, J.D. Clark, and J.M. Bewley. 2015. Effect of alley-floor scraping frequency on Escherichia coli, Klebsiella species, environmental Streptococcus species, and coliform counts. The Professional Animal Scientist 31(3):284-289.

Wadsworth, B.A., A.E. Stone, J.D. Clark, D.L. Ray, and J.M. Bewley. 2015. Stall cleanliness and stall temperature of two different freestall bases. J. Dairy Sci. 98 (6): 4206-4210.

University of Idaho:

Rezamand, P., B. P. Hatch, K. G. Carnahan, and M. A. McGuire. 2016. Effects of α-linolenic acid-enriched diets on gene expression of key inflammatory mediators in immune and milk cells obtained from Holstein dairy cows. J. Dairy Res. 83: 20–27.

Louisiana State University:

University of Maryland:

2016. Garcia, T.H. Elasser, L. Juengst, Y. Qu, B.J. Bequette and K.M. Moyes. 2016. SHORT COMMUNICATION: Amino acid supplementation and stage of lactation alter   

apparent utilization of nutrients by blood neutrophils from lactating dairy cows in

vitro.  J. Dairy Sci. 99:3777-3783.

K.M. Moyes, P. Sørensen and M. Bionaz^.  2016.  The impact of intramammary Escherichia coli challenge on liver and mammary transcriptome and cross-talk in dairy cows during early lactation using RNAseq. PLOSOne. 23. doi:10.1371/journal.pone.0157480. eCollection 2016.

M.A. Crookenden, A. Heiser, A. Murray, V.S.R. Dukkipati, J.K. Kay, J.J. Loor^, S.

Meirer, M.D. Mitchell, K.M. Moyes, C.G. Walker and J.R. Roche.  2016.    

Parturition in dairy cows temporarily alters the expression of genes in circulating neutrophils. 

6483. Dairy Sci. 99:6470-6483.

M.A. Crookenden, C.G. Walker, A. Heiser, A. Murray, V.S.R. Dukkipati, J.K. Kay, S. Meier, K.M. Moyes, M.D. Mitchell, J.J. Loor^ and J. R. Roche.  2016. Effects of precalving body condition and prepartum feeding level on gene expression in circulating neutrophils.  J. Dairy Sci.  Accepted.

Michigan State University:

Sordillo, L. M. 2016. Nutritional strategies to optimize dairy cattle immunity. J. Dairy Sci. 99:4967-4982.

Mavangira, V., M. J. Mangual, J. C. Gandy, L. M. Sordillo. 2015. 15-F2t-Isoprostane Concentrations and Oxidant Status in Lactating Dairy Cattle with Acute Coliform Mastitis. J Vet Intern Med. 30:339-347.

Boutinaud, M., N. Isaka, V. Lollivier, F. Dessauge, E. Gandemer, P. Lamberton, A. I. De Prado Taranilla, A. Deflandre, L.M. Sordillo. 2016. Cabergoline inhibits prolactin secretion and accelerates involution in dairy cows after dry-off. J. Dairy Sci. 99:5707-5718.

University of Minnesota:

Godden, S.M., E. Royster, W. Knauer, Y. Schukken, M. Lopez-Benavides, J. Sorg, S. Leibowitz, and E.A. French. 2016. Randomized non-inferiority study evaluating the efficacy of a post-milking teat disinfectant for the prevention of naturally occurring intramammary infections. J. Dairy Sci. 99:3675-3687.

University of Missouri:

Hoernig KJ, Donovan DM, Pithua P, Williams III F, Middleton JR.  2016.  Evaluation of a lysostaphin-fusion protein as a dry-cow therapy for Staphylococcus aureus mastitis in dairy cattle.  J Dairy Sci.  99(6):4638-4646.  [Epub 2016 Mar 31]

Adkins PRF, Middleton JR, Fox LK^.  2016.  Comparison of virulence gene identification, ribosomal spacer PCR genotype, and pulsed-field gel electrophoresis for strain-typing Staphylococcus aureus isolated from cases of subclinical bovine mastitis in the USA.  J Clin Microbiol.  54(7):1871-1876.  [Epub 2016 May 18]

Rutgers University (New Jersey):

Rosario-Cruz Z., Chahal H.K., Anzaldi-Mike L.L., Skaar E.P., and Boyd J.M.  Bacillithiol has a role in Fe-S cluster biogenesis in Staphylococcus aureus.  Mol. Micro. 2015 Oct. PMID: 26135358

Mashruwala A.M., and Boyd J.M., De novo assembly of plasmids using yeast recombinational cloning.  Methods Mol. Biol. 2016 Feb PMID: 26194707

Rosario-Cruz Z. and Boyd J.M.  Pysiological roles of bacillithiol in intracellular metal processing.  Curr Genet.  2016 Feb.  PMID: 26259870

Mashruwala A.A., Bhatt S., Poudel S., Boyd E.S., and Boyd J.M. The DUF59 containing protein SufT is involved in the maturation of iron-sulfur (FeS) proteins during conditions of high FeS cofactor demand in Staphylococcus aureus. PLoS Genetics. 2016 Aug. PMID: 27517714

Choby J.E., Mike L.A., Mashruwala A.A., Dutter B.F.  Dunman, P.M., Sulikowski G.A., Boyd J.M., and Skaar E.P.* A small molecule inhibitor of iron-sulfur cluster assembly is toxic to Staphylococcus aureus in an Sae-dependent manner. Cell Chemical Biology 2016 Nov. PMID:27773628

Mashruwala A.A., Roberts C., Bhatt S. May K.L., Carroll R.K., Shaw L.N., Boyd J.M. Staphylococcus aureus SufT: an essential iron-sulfur cluster assembly factor in cells experiencing a high-demand for lipoic acid.  Mol. Micro. 2016 Sep. PMID: 27671355

Cornell University (New York):

Addis MF, Tedde V, Puggioni GM, Pisanu S, Casula A, Locatelli C, Rota N, Bronzo V, Moroni P, Uzzau S. Evaluation of milk cathelicidin for detection of bovine mastitis. J Dairy Sci. 2016 Oct;99(10):8250-8. doi:10.3168/jds.2016-11407. PubMed PMID: 27522416.

Ospina PA, Rota N, Locatelli C, Colombo L, Pollera C, Giacinti G, Bronzo V, Casula A, Arpinelli A, Brossette V, Facchi M, Patelli A, Ruggeri A, Barberio A, Potenza G, Nydam DV, Moroni P. Randomized noninferiority field trial comparing 2 first-generation cephalosporin products at dry off in quarters receiving an internal teat sealant in dairy cows. J Dairy Sci. 2016 Aug;99(8):6519-31. doi: 10.3168/jds.2015-10807. PubMed PMID: 27265178.

Addis MF, Tanca A, Uzzau S, Oikonomou G, Bicalho RC, Moroni P. The bovine milk microbiota: insights and perspectives from -omics studies. Mol Biosyst. 2016 Jul 19;12(8):2359-72. doi: 10.1039/c6mb00217j. Review. PubMed PMID: 27216801.

Barberio A, Flaminio B, De Vliegher S, Supré K, Kromker V, Garbarino C, Arrigoni N, Zanardi G, Bertocchi L, Gobbo F, Catania S, Moroni P. Short communication: In vitro antimicrobial susceptibility of Mycoplasma bovis isolates identified in milk from dairy cattle in Belgium, Germany, and Italy. J Dairy Sci. 2016 Aug;99(8):6578-84. doi: 10.3168/jds.2015-10572. PubMed PMID: 27209138.

Gioia G, Werner B, Nydam DV, Moroni P. Validation of a mycoplasma molecular diagnostic test and distribution of mycoplasma species in bovine milk among New York State dairy farms. J Dairy Sci. 2016 Jun;99(6):4668-77. doi:10.3168/jds.2015-10724. PubMed PMID: 27016831.

Bardiau M, Caplin J, Detilleux J, Graber H, Moroni P, Taminiau B, Mainil JG. Existence of two groups of Staphylococcus aureus strains isolated from bovine mastitis based on biofilm formation, intracellular survival, capsular profile and agr-typing. Vet Microbiol. 2016 Mar 15;185:1-6. doi:10.1016/j.vetmic.2016.01.003. PubMed PMID: 26931384.

Locatelli C, Cremonesi P, Bertocchi L, Zanoni MG, Barberio A, Drigo I, Varisco G, Castiglioni B, Bronzo V, Moroni P. Short communication: Methicillin-resistant Staphylococcus aureus in bulk tank milk of dairy cows and effect of swine population density. J Dairy Sci. 2016 Mar;99(3):2151-6. doi: 10.3168/jds.2015-9940. PubMed PMID: 26805972.

Scaccabarozzi L, Leoni L, Ballarini A, Barberio A, Locatelli C, Casula A, Bronzo V, Pisoni G, Jousson O, Morandi S, Rapetti L, García-Fernández A, Moroni P. Pseudomonas aeruginosa in Dairy Goats: Genotypic and Phenotypic Comparison of  Intramammary and Environmental Isolates. PLoS One. 2015 Nov 25;10(11):e0142973. doi: 10.1371/journal.pone.0142973. PubMed PMID: 26606430; PubMed Central PMCID: PMC4659641.

Plumed-Ferrer C, Barberio A, Franklin-Guild R, Werner B, McDonough P, Bennett J, Gioia G, Rota N, Welcome F, Nydam DV, Moroni P. Antimicrobial susceptibilities and random amplified polymorphic DNA-PCR fingerprint characterization of Lactococcus lactis ssp. lactis and Lactococcus garvieae isolated from bovine intramammary infections. J Dairy Sci. 2015 Sep;98(9):6216-25. doi: 10.3168/jds.2015-9579. PubMed PMID: 26142865.

Kessels JA, Cha E, Johnson SK, Welcome FL, Kristensen AR, Gröhn YT. Economic comparison of common treatment protocols and J5 vaccination for clinical mastitis in dairy herds using optimized culling decisions. J Dairy Sci. 2016 May;99(5):3838-47. doi: 10.3168/jds.2015-10385. PubMed PMID: 26947301.

Cha E, Hertl J, Schukken Y, Tauer L, Welcome F, Gröhn Y. Evidence of no protection for a recurrent case of pathogen specific clinical mastitis from a previous case. J Dairy Res. 2016 Feb;83(1):72-80. doi: 10.1017/S002202991500062X. PubMed PMID: 26568557.

Troendle, J.A., Tauer, L.W.,  Gröhn, Y.T.: Optimally achieving milk bulk tank somatic cell count thresholds. Journal of Dairy Science. Accepted.

Oregon State University:

Bionaz, M., et al. (2015). "TRIENNIAL LACTATION SYMPOSIUM: Nutrigenomics in dairy cows: Nutrients, transcription factors, and techniques." J Anim Sci 93(12): 5531-5553.

Pennsylvania State University:

Schewe, R.L., J. Kayitsinga, G.A. Contreras, C. Odom, W.A. Coats, P. Durst, E.P. Hovingh, R.O. Martinez, R. Mobley, S. Moore, R.J. Erskine^.  Herd management and social variables associated with bulk tank somatic cell count in dairy herds in the eastern United States. 2015. J. Dairy Sci. 98(11).7650-7665.

Utah State University:

Wilson DJ, Rood KA, Whitehouse C, Bunnell J, Goodell GM, Byrem TM: Dairy herd -  level prevalence of Johne’s disease and BVD in the Intermountain West of the U.S.A. and farm management practices and characteristics for test-positive herds. J Veterinary Sci Technol 6:4:1-7, 2015.

University of Vermont:

Carroll, J.A., N.C. Burdick-Sanchez, J.D. Arthingon, C.D. Nelson, A.L. Benjamin, F.T. Korkmaz, D.E. Kerr, and P.A. Lancaster. 2017. In utero exposure to lipopolysaccharide alters the postnatal acute phase response in beef heifers. Innate Immunity 23: In Press

Benjamin A.L., F.T. Korkmaz, T.H. Elsasser, and D.E. Kerr. 2016. Neonatal LPS exposure does not diminish the innate immune response to a subsequent LPS challenge in Holstein bull calves. Journal of Dairy Science. 99:5750-5763.

Benjamin A.L., B.B. Green, S.D. McKay, B.A. Crooker, and D.E. Kerr. 2016. Differential responsiveness of Holstein and Angus dermal fibroblasts to LPS challenge occurs without major differences in the methylome. BMC Genomics. 17:[Epub March 24 ahead of print].

Virginia Tech:

Langel SN, Wark WA, Garst SN, James RE, McGilliard ML, Petersson-Wolfe CS, Kanevsky-Mullarky I. 2016.  Effect of feeding whole compared with cell-free colostrum on calf immune status: Vaccination response.  J. Dairy Sci.  99(5):3979-94.

Ludwig EK, Brandon Wiese R, Graham MR, Tyler AJ, Settlage JM, Werre SR, Petersson-Wolfe CS, Kanevsky-Mullarky I, Dahlgren LA. 2016.  Serum and synovial fluid serum amyloid A response in equine models of synovitis and arthritis.  Vet Surg. 45(7): 859-867.  

Swartz TH, McGilliard ML, Petersson-Wolfe CS. 2016.  The use of an accelerometer for measuring step acitivty and lying behaviors in dairy calves.  J. Dairy Sci. 99(11):9109-9113. 

Christina Petersson-Wolfe and Michelle Arnold.  Reference guide for mastitis-causing bacteria.  http://sequalitymilk.com/wp-content/uploads/2016/04/SQMI-Bacterial-Reference-Guide.pdf

Christina Petersson-Wolfe and Turner Swartz.  Yeast and Molds: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-72/DASC-72.html

Christina Petersson-Wolfe and Turner Swartz.  Trueperella pyogenes: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-71/DASC-71.html

Christina Petersson-Wolfe and Turner Swartz.  Proteus spp.: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-68/DASC-68.html

Christina Petersson-Wolfe and Turner Swartz.  Pasteurella spp.: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-67/DASC-67.html

Christina Petersson-Wolfe and Turner Swartz.  Mycoplasma spp.: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-66/DASC-66.html

Christina Petersson-Wolfe and Turner Swartz.  Enterobacter spp.: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-65/DASC-65.html

Christina Petersson-Wolfe and Turner Swartz.  Corynebacterium bovis: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-64/DASC-64.html

Christina Petersson-Wolfe and Turner Swartz.  Coagulase-negative staphylococci and Staphylococcus hyicus: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-63/DASC-63.html

Christina Petersson-Wolfe and Turner Swartz.  Bacillus spp.: A practical summary for controlling mastitis.  https://pubs.ext.vt.edu/DASC/DASC-62/DASC-62.html

Washington State University:

Adkins P.R.F, John R Middleton^; Lawrence K Fox. 2016. Staphylococcus aureus Strains Isolated from Cases of Subclinical Bovine Mastitis in the United States.  Journal of Clinical Microbiology. 54: 1871-1876

Nicholas, R.A.J., L.K. Fox, I. Lysnyansky. 2016.  Mycoplasma mastitis in cattle: to cull or not to cull.  The vet. J. 216:142-147.

Fox, L.K. 2016.  Removal of hair from the mammary gland: Recovery of bacteria from teat skin and milk.  Journal of Dairy Science. 99:1461-1464.

University of Wisconsin:

Guarin, J.F. and P.L. Ruegg.  2016.  Short Communication:  Pre- and post-milking anatomical characteristics of teats and their associations with increased risk of clinical mastitis in dairy cows.  J Dairy Sci. 99:8323-8329.    

Guarin, J.F., M.G. Paixao, and P.L. Ruegg.  2016.  Association of anatomical characteristics of teats with quarter milk somatic cell count.  Published online  27 July, 2016:  http://dx.doi.org/10.3168/jds.2016-11459.   

Guarin, J.F., C. Baumberger, and P.L. Ruegg.  Anatomical characteristics of teats and pre-milking bacterial counts of teat skin swabs of primiparous cows exposed to different types of bedding. Accepted J Dairy Sci.  Oct  14. 2016

International:

University of Montreal

Belage, E., Dufour, S., Jones-Bitton, A., Schock, D., Kelton, D.F., Submitted for publication. Adoption and consistency of application of pre-milking preparation in Ontario dairy herds. Journal of  Dairy Science.

Francoz, D., Welllemans, V., Roy, J.P., Lacasse, P., Ordonez-Iturriaga, A., Labelle, F., Dufour, S., Accepted for publication. Non-antibiotic approaches at drying off for treating and preventing intramammary infections: A protocol for a systematic review and meta-analysis. Animal Health Research Reviews.

Goetz, C., Dufour, S., Archambault, M., Malouin, F., Jacques, M., 2016. Importance et contrôle de biofilms formés par les staphylocoques lors d’infections intra-mammaires chez la vache laitière. Revue de Médecine Vétérinaire 167, 215-229.

Abstracts

University of Georgia:

Alward, K. J., J. F. Bohlen, L. O. Ely, and S. C. Nickerson. 2016. Assessing the correlation between teat end scores and the presence of mastitis in lactating Holstein cows. Abstract. Joint Meeting of the American Dairy Science Association and the American Society of Animal Science; 2016 July 19-23, 2016, Salt Lake City, UT. Page 22 in: J. Anim. Sci. Vol. 94, E-Suppl.5/J. Dairy Sci. Vol. 99, E-Suppl. 1.

Enger, B. D., R. R. White, S. C. Nickerson, and L. K. Fox^. 2016. Meta- analysis of factors influencing new intramammary infection rate in experimental challenge teat dip efficacy trials. ADSA.  Page 57 in: J.       Anim. Sci. Vol. 94, E-Suppl. 5/J. Dairy Sci. Vol. 99, E-Suppl. 1.

Enger, B. D., R. R. White, S. C. Nickerson, and L. K. Fox^. 2016.  Meta-analysis of factors influencing new intramammary infection rate in natural exposure teat dip efficacy trials. ADSA. Page 355 in: J. Anim. Sci. Vol. 94, E-Suppl. 5/J. Dairy Sci. Vol. 99, E-Suppl. 1.

Hall, C.L., S.C. Nickerson, D.J. Hurley, L.O. Ely, and F.M. Kautz. 2015. Use of a novel adjuvant to enhance the protective effect of a commercial Vaccine against Staphylococcus aureus mastitis in dairy heifers.  J. Dairy Sci. Abstract no. 514. Orlando, FL. Page 569 in: J. Anim. Sci. Vol. 93, Suppl. s3/J.

Dairy Sci. Vol. 98, Suppl. 2. http://www.jtmtg.org/JAM/2015/abstracts/566.pdf.

Hurley, D.J., M. Adkins, C. Barber, N.A. Norton, S.C. Nickerson, L.O. Ely, F.M. Kautz, D.J. McClean, J.D. Chapman, and A.D. Rowson. 2016. Cell-mediated immune response to MLV BRD vaccination in Holstein heifers fed an immunomodulatory supplement. 2016 CRWAD. Abstract.

Kautz, F.M., S.C. Nickerson, and L.O. Ely. 2105. Use of a staphylococcal vaccine to reduce prevalence of mastitis and lower somatic cell counts in a registered Saanen dairy goat herd. J. Dairy Sci. Abstract no. 627. Orlando, FL. Page 613 in: J. Anim. Sci. Vol. 93, Suppl. s3/J. Dairy Sci. Vol. 98, Suppl. 2. http://www.jtmtg.org/JAM/2015/abstracts/613.pdf.

Pighetti, G.M., C.S. Petersson-Wolfe^, J.M. Bewley^, S.C. Nickerson, S. Hill-Ward, A. De Vries, and S.P. Oliver. 2015. Southeast Quality Milk Initiative: Milk quality in the Southeast USA. National Mastitis Council Annual Meeting Proceedings, pp 213-214. Memphis, TN. February 1-3, 2015.

Pighetti, G.M., S.P. Oliver, R.A. Almeida, P.D. Krawczel, J.M. Fly, S.M. Schexnayder, C.S. Petersson-Wolfe^, J.M. Bewley^, L.E. Garkovich, D.M. Amaral-Phillips, L.M. Arnold, S.C. Nickerson, S. Hill-Ward, and A. De Vries.  2015.  Southeast Quality Milk Initiative: Milk Quality in the Southeast USA.  NMC Annual Meeting Proceedings, pp 209-210. Memphis, TN. February 1-3, 2015.

University of Idaho:

2016. Chen, K. C. Ramsey, C. Y. Tsai, M. A. McGuire, and P. Rezamand. 2016. Interaction among energy status, dietary protein, and vitamin A in periparturient dairy cows: Effects on milk fatty acid profile and gross milk yield efficiency. Dairy Sci. Vol. 99, (E-Suppl. 1):404.

University of Kentucky:

Bochantin, K. and J.M. Bewley. 2016. The importance of mastitis management practices in maintaining milk quality in the United States. Abstract 42. American Dairy Science Association Annual Meeting. Salt Lake City, Utah.

Nolan, D.T., C. Blakely, P. D. Krawczel, C. S. Petersson-Wolfe^, G. M. Pighetti, A. Stone, S. Ward, and J. M. Bewley. 2016. Perceived mastitis costs and milk quality management practices among Southeastern United States dairy producers. Abstract 585. American Dairy Science Association Annual Meeting. Salt Lake City, Utah.

Stone, A.E., C. Blakely, K. Bochantin, P. D. Krawczel, M. Myers, D. T. Nolan, C. S. Petersson-Wolfe^, G. M. Pighetti, S. Ward, and J. M. Bewley. Housing and demographic effects on somatic cell score in southeast United States dairies. Abstract 761. American Dairy Science Association Annual Meeting. Salt Lake City, Utah.

Lee, A.R., S.M. Smith, D.L. Ray, J.D. Clark, and J.M. Bewley. 2016. Effects of exit-lane water drenching using showers on lactating dairy cow vaginal temperature.     Abstract 1174. American Dairy Science Association Annual Meeting. Salt Lake City, Utah.

Nolan, D.T., J.M. Bewley. 2015. The effect of somatic cell score on milk yield of dairy cattle in the southeastern United States.  Abstract 93. Dairy Science Association Annual Meeting. Orlando, FL. (Oral)

Kawonga, B.S. and J.M. Bewley, 2015.  A cow cooling investment decision support tool for dairy farms in low and high humidity regions.  Abstract No. 813. American Dairy Science Association and American Society of Animal Science Joint Annual Meeting, July 12-16. Orlando Florida.

Eckelkamp, E.A., J.L. Taraba, R.J. Harmon, K.A. Akers, and J.M. Bewley. 2015. Comparison of mastitis, its indicators, and lameness in compost bedded pack and sand freestall farms.  European Federation of Animal Science Annual Meeting. Warsaw, Poland.

Bewley, J.M., R.A. Black, F.A. Damasceno, E.A. Eckelkamp, G.B. Day, and J.L. Taraba. 2015. Compost bedded pack barns as a lactating cow housing system. European Federation of Animal Science Annual Meeting. Warsaw, Poland.

Eckelkamp, E.A., J.L. Taraba, R.J. Harmon, K.A. Akers, and J.M. Bewley. 2015. Moisture, temperature, cow health, and bedding bacteria relationships in compost bedded pack barns. Abstract 275. American Dairy Science Association Annual Meeting. Orlando, FL.

Eckelkamp, E.A., J.L. Taraba, R.J. Harmon, K.A. Akers, and J.M. Bewley. 2015. Effect of compost bedded pack and sand bedded freestall housing on reported clinical mastitis incidence, mastitis infection prevalence, herd somatic cell count, and bulk tank somatic cell count. Abstract 4. National Mastitis Council 54th Annual Meeting Technology Transfer Session.  Memphis, TN.

University of Maryland:

2016. Garcia, Y. Qu, C.M. Scholte, D. O’Connor, W. Rounds and K.M. Moyes. 2016.

Regulatory effect of dietary intake of chromium propionate on function of monocyte-derived macrophages from Holstein cows in mid-lactation.  J. Dairy Sci. 99(Suppl 1): 737.

2016. Qu, T.H. Elsasser, M. Garcia, C.M. Scholte, E.E. Connor, J. Newbold and K.M. Moyes. 2016. The effects of short-term feeding of tocopherol mix (α-, β-, γ-, and δ) on blood neutrophil function and immunometabolic-related gene expression in lactating dairy cows.  J. Dairy Sci. 99(Suppl 1):57.

Scholte, C.M., Y. Qu, M. Garcia, T.H. Elsasser, D. Biswas and K.M. Moyes. 2016.

Effects of citral and linalool on blood neutrophil toxicity and oxidative response in dairy cows.   J. Dairy Sci. 99(Suppl 1):327.

M.A. Crookenden, C.G. Walker, A. Heiser, J.J. Loor^, K.M. Moyes, J.K. Kay, S. Meier,

2016. Murray, V.S.R. Dukkipati, M. Mitchell and J.R. Roche. 2016. miRNAseq from whole blood over the transition period. J. Dairy Sci. 99(Suppl 1):85.

University of Minnesota:

Godden, S.M., E. Royster, J. Timmerman, P. Rapnicki, and H. Green. 2016. Evaluation of an Automated Milk Leukocyte Differential Test for Detecting Intramammary Infection in Early and Late Lactation Quarters and Cows. Annu Mtg. of the American Association of Bovine Practitioners. Sept. 15-17, 2016. Charlotte, NC

Patel, K., S. Godden, E. Royster, J. Timmerman, B. Crooker, and N. McDonald. 2016. Pilot study: Evaluation of the effect of selective dry cow therapy on udder health.  In: Proc. Annu. Meeting of the Minnesota Dairy Health Conference. May 18-20, 2016. Bloomington, MN.

University of Missouri:

Adkins PRF, Middleton JR, Fox LK^.  2016.  Potential body site reservoirs for coagulase negative staphylococcal intramammary infection in heifers.  J Animal Sci.  94(supplement 2):33.

Quas ED, Bernier Gosselin V, Adkins PRF, Middleton JR.  2016.  Identifying coagulase negative staphylococcal species Isolated from dairy goat milk using MALDI-TOF Mass Spectrometry.  Proceedings of the 39^th Annual CVM Research Day (Phi Zeta).  6 May 2016.  Abstract #6 – Veterinary Professional Student Oral Presentations.

Limberg E, Adkins PRF, Middleton JR.  2016.  Effect of freezing and storage time on culture yields of body site swabbing samples from dairy heifers.  Proceedings of the 39^th Annual CVM Research Day (Phi Zeta).  6 May 2016.  Abstract #21 – Veterinary Professional Student Poster Presentations.

Bernier Gosselin V, Middleton JR, Quas E, Adkins PRF.  2016.  Persistence of intramammary infection by coagulase negative staphylococcal species in dairy goats.  Proceedings of the 39^th Annual CVM Research Day (Phi Zeta).  6 May 2016.  Abstract #19 – 2^nd and 3^rd Year Resident & Graduate Student Oral Presentations.

Arroyo G, Adkins PRF, Fox LK^, Middleton JR.  2016.  Comparison of MALDI-TOF and PFGE for strain-typing Staphylococcus aureus isolated from cow’s milk.  Veterinary Research Scholars Symposium, Ohio State University, 28-31 July 2016.

Cornell University (New York):

Troendle, J., Tauer, L.W.,  Gröhn, Y.T.: Optimally achieving milk bulk tank somatic cell count thresholds, 2016 AAEA Annual Meeting in Boston, MA, July 31-August 2. 

Oregon State University:

Richards, S. G., et al. (2014). "Effect of 2,4-thiazolidinedione treatment in milk production and leukocytes phagocytosis after sub-clinical mastitis induction in lactating dairy goats." J Dairy Sci 97(E-Suppl. 1): 419-420.

Rosa, F., et al. (2015). 2,4-thiazolidinedione improves liver function but does not affect insulin sensitivity and expression of genes in adipose and mammary tissue of lactating dairy goats. 2015 Joint Annual Meeting, Orlando, FL.

Rosa, F., et al. (2016). Effect of 2,4-thiazolidinedione treatment in the inflammatory response to induced sub-clinical mastitis in dairy goats receiving adequate vitamin supplementation. 2016 Joint Annual Meeting, Salt Lake City, UT.

University of Vermont:

Korkmaz, F.T. and D.E. Kerr. 2016.  Identifying DNA methylation Differences That Contribute to an Age-Dependent Increase in Bovine Innate Immunity Using Reduced Representation Bisulfite Sequencing and the Dermal Fibroblast Model. 35th International Society for Animal Genetics Conference. Salt Lake, UT.

Mugabi, R. and Barlow, J.W. 2015. Biofilm formation is clonally specific among Staphylococcus aureus isolated from selected Vermont dairy farms. 116th General Meeting of the American Society for Microbiology, Boston, Massachusetts.

Washington State University:

Arroyo, G.T., P.R. Adkins, L.K. Fox, J.R. Middleton^. 2016.  Comparison of MALDI-Tof and PFGE for strain-typing Staphylococcus aureus isolated from cow’s milk Merial 27^th Annual Veterinary Scholars Symposium, The Ohio State University, Columbus, OH.

Enger, B.D., R.R. White, S.C. Nickerson^, and L.K. Fox. 2016.  Factors influencing new intramammary infection rate in teat dip efficacy trials by meta-analysis. Proceedings of the NMC 55th Annual Meeting, Phoenix, AZ. pages 100-102

Enger,B.D., R. R. White, S. C. Nickerson^, and L. K. Fox. 2016.  Meta-analysis of factors influencing new intramammary infection rate in experimental challenge teat dip efficacy trials. J. Dairy Sci. Vol. 99, E-Suppl. 1, number 126.

Enger,B.D., R. R. White, S. C. Nickerson^, and L. K. Fox. 2016.  Meta-analysis of factors influencing new intramammary infection rate in natural exposure  teat dip efficacy trials. J. Dairy Sci. Vol. 99, E-Suppl. 1, number 754.

Conference Proceedings

University of Georgia:

Nickerson, S. C. 2016. Benefits of fly control in dairy heifers. Invited Symposium Paper presented in: Strategies for Managing Heifers in the Southeast. ADSA Annual Meeting, Salt Lake City, UT. July 20, 2016. Page 26 in: J. Anim. Sci. Vol. 94, E-Suppl. 5/J. Dairy Sci. Vol. 99, E-Suppl. 1.

University of Maine:

Lichtenwalner, A. Bedding Choices for Mastitis Prevention.  Feb. 2-4, 2016. Mastitis Research Workers Conference, Glendale AZ.

University of Maryland:

C.M. Scholte, D.C. Nelson, T.H. Elsasser, S. Kahl, E.E. Connor, Y. Qu and K.M. Moyes.  2016.  Recombinant bacteriophage endolysin, PlyC, is non-toxic and does not alter blood neutrophil oxidative response in lactating dairy cows.  in Natl. Mastitis Counc. Ann.  Mtg. Proc., Glendale, AZ.  Natl. Mastitis Counc., Inc., Madison, WI.

Michigan State University:

Erskine, R.J.  How Can We Engage Dairy Employees? Southeast Quality Milk Initiative Annual Meeting, Russellville, KY, November, 2015.

Erskine R. J. The Quality Milk Alliance: Building the science classroom. Seminar presented to the General Session, 55^th Annual Mtng National Mastitis Council, Glendale, AZ, February, 2016.

Erskine, R. J., M. Borek-Stine, and R. Moore. Engaged employees: The connection between protocols and performance. Short course presented at the 55^th Annual Mtng National Mastitis Council, Glendale, AZ, February, 2016.

Erskine, R.J. Let the Cows Vote on Milking Protocols. Presented to The Progressive Dairy Operators of Canada, Toronto, ON, March, 2016.   

Erskine, R.J. 1) Applying pharmacology on a dairy farm, 2) Dumpster diving, somatic cell counts, and employee incentives: A Quality Milk Alliance potpourri, and 3) Bovine Leukemia Virus, is it a problem? Or not?  Seminars presented to the Pennsylvania Veterinary Medical Association, Spring Clinic, State College, PA, May, 2016.  

University of Minnesota:

Godden, S., E. Royster, K. Patel, and J. Timmerman. 2016. Selective dry cow therapy – We can do this! Proc. National Mastitis Council 2016 Regional Meeting. June 29-30, 2016. Appleton, WI.  Pp. 8-15.

University of Missouri:

Adkins PRF, Middleton JR.  2016.  Molecular characterization of coagulase negative staphylococci from heifer intramammary infections and potential body site reservoirs.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Bernier Gosselin V, Adkins PRF, Middleton JR.  2016.  Prevalence of coagulase negative staphylococcal species intramammary infection in dairy goats.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Lopez-Benavides M, Romero-Saurez S, Henderson M, Adkins EC, Denbigh J, Middleton JR.  2016.  Iodide residues in bulk tank milk when using iodine and non-iodine pre-milking teat disinfectants.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Lopez-Benavides M, Vandaveer W, Leibowitz S, Adkins EC, Denbigh J, Middleton JR.  2016.  Glycolic acid is present in bulk tank milk when using non-glycolic acid and glycolic acid based teat disinfectants.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Cornell University (New York):

Moroni P., Gioia G., Kolar Q., Mock L., Ospina P., Plumed-Ferrer C., Rauch B., Santisteban C., Scillieri Smith J., Virkler P., Watters R., Welcome F., Zurakoswski M., and Nydam D. Emerging pathogens: the last information on Klebsiella, Prototheca and Lactococci. Proceeding of the 54^nd  National Mastitis Council Annual Meeting pp.37- 49. February 1-3, 2015, Menphis, Tennesse.

Short course “Emerging mastitis pathogens: Klebsiella, Prototheca, Mycoplasma and Other Streptococci. National Mastitis Council Regional Meeting pp.10-16. July 28-29, 2015, Syracuse, New York.

Pennsylvania State University:

Hovingh, E.P.  Use of a four-channel vacuum recording device for monitoring and diagnosing milk equipment and milking procedures abnormalities. 2016. Florida Dairy Summit, Gainesville, FL.  February 11, 2016. (Conference presentation)

Utah State University:

Britten JE, Wilson DJ, Rood KA: The intramammary use of casein hydrolysate for cessation of lactation in a single quarter. Proc 55th Ann Mtg Natl Mast Council:120-121, 2016.

Wilson DJ, Rood KA, Whitehouse C, Bunnell J, Goodell GM, Byrem TM: Johne’s disease and Bovine Viral Diarrhea bulk milk surveillance in the Western U.S. - regional prevalence and positive farm characteristics. Proc XXIX World Assoc Buiatrics: 369, 2016.

Kelly EJ, Wilson DJ: Pseudomonas aeruginosa mastitis in two goats associated with contaminated essential-oil based teat dip. Proc 59th Ann Conf Am Assoc Vet Lab Diag: 181, 2016.

Wilson DJ, Rood KA, LaRose JP, Wang Z: Holstein single nucleotide polymorphisms analyzed by genome wide association study for associations with mastitis resistance and susceptibility. Proc 59th Ann Conf Am Assoc Vet Lab Diag: 70, 2016.

University of Wisconsin:

Fuenzalida, M. J., C. Baumberger, and P. L. Ruegg. 2016. Preliminary results of a clinical trial evaluating effects of treatment of culture negative cases of clinical mastitis on somatic cell count and bacteriological outcomes. Pages112—113 in National Mastitis Council Annual Meeting Proceedings, Glendale, AZ.

International:

University of Montreal

Francoz, D., Wellemans, V., Dupré, J.P., Roy, J.P., Labelle, F., Karreman, H., Lacasse, P., Dufour, S., 2016. A systematic review of non-antimicrobial treatments of clinical mastitis in dairy cows. In, 2nd Canadian organic science conference, Montréal-Longueuil, QC, Canada.

Francoz, D., Wellemans, V., Dupré, J.P., Roy, J.P., Labelle, F., Karreman, H., Lacasse, P., Dufour, S., 2016. A systematic review of non-antimicrobial treatments of clinical mastitis in dairy cows. In, World buiatric congress, Dublin, Ireland.

Jamali, H., Barkema, H.W.^, Jacques, M., Lavallée-Bourget, E.M., Malouin, F., Saini, V., Stryhn, H., Dufour, S., 2016. A systematic review and meta-analysis of clinical mastitis recurrence in dairy cows. In, World buiatric congress, Dublin, Ireland.

Jamali, H., Barkema, H.W.^ Jacques, M., Malouin, F., Saini, V., Stryhn, H., Dufour, S., 2016. Clinical mastitis recurrence in dairy cows. In, NMC 55^th annual meeting, Glendale, Arizona, USA.

Belage, E., Kelton, D.F., Bauman, C., Dufour, S., 2016. National dairy study - A focus on Udder Health and Milking management on Canadian dairy farms. In, The 2016 meeting of the Canadian Association of Veterinary Epidemiology and Preventive Medicine, Guelph, ON, Canada.

Poster Presentations

University of Georgia:

Enger, B.D., R.R. White, S.C. Nickerson, and L.K. Fox^. 2016. Factors influencing new intramammary infection rate in teat dip efficacy trials by meta-analysis. Poster and oral presentation. National Mastitis Council Technology Transfer Session and Research Development Session. NMC 55^th Annual Meeting, January 31-February 2, 2016, Glendale, Arizona.

University of Idaho:

2016. Chen, K. C. Ramsey, C. Y. Tsai, M. A. McGuire, and P. Rezamand. 2016. Interaction among energy status, dietary protein, and vitamin A in periparturient dairy cows: Effects on milk fatty acid profile and gross milk yield efficiency. The 2016 Joint Annual Meetings, Salt Lake, Utah.

Michigan State University:

Moore, R. and R. J. Erskine. Let the cows score the milking protocols. Poster presented at the 55^th Annual Mtng National Mastitis Council, Glendale, AZ, February,2016.

University of Minnesota:

Patel, K., S. Godden, E. Royster, J. Timmerman, B. Crooker, and N. McDonald. Pilot study: Evaluation of the effect of selective dry cow therapy on udder health. University of Minnesota College of Veterinary Medicine Points of Pride Research Day. Oct. 5, 2016.

University of Missouri:

Limberg E, Adkins PRF, Middleton JR.  2016.  Effect of freezing and storage time on culture yields of body site swabbing samples from dairy heifers.  Proceedings of the 39^th Annual CVM Research Day (Phi Zeta).  6 May 2016.  Abstract #21 – Veterinary Professional Student Poster Presentations.

Arroyo G, Adkins PRF, Fox LK^, Middleton JR.  2016.  Comparison of MALDI-TOF and PFGE for strain-typing Staphylococcus aureus isolated from cow’s milk.  Veterinary Research Scholars Symposium, Ohio State University, 28-31 July 2016.

Adkins PRF, Middleton JR.  2016.  Molecular characterization of coagulase negative staphylococci from heifer intramammary infections and potential body site reservoirs.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Bernier Gosselin V, Adkins PRF, Middleton JR.  2016.  Prevalence of coagulase negative staphylococcal species intramammary infection in dairy goats.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Lopez-Benavides M, Romero-Saurez S, Henderson M, Adkins EC, Denbigh J, Middleton JR.  2016.  Iodide residues in bulk tank milk when using iodine and non-iodine pre-milking teat disinfectants.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Lopez-Benavides M, Vandaveer W, Leibowitz S, Adkins EC, Denbigh J, Middleton JR.  2016.  Glycolic acid is present in bulk tank milk when using non-glycolic acid and glycolic acid based teat disinfectants.  Proceedings of the 55^th Annual Meeting of the National Mastitis Council, Glendale, AZ.  Jan 31 – Feb 2.

Rutgers University (New Jersey):

Poster. Rosario-Cruz Z*., Gandhi S., Boyd JM.  Copper homeostasis in Staphylococcus aureus. American Society of Microbiology National Meeting. New Orleans, LA  2015.

Poster. Rosario-Cruz Z*., Gandhi S., Boyd JM.  Copper homeostasis in Staphylococcus aureus. Meeting of the New Jersey Antimicrobial Resistance Working Group. Piscataway, NJ  2015.

Poster. Mashruwala A.A., van de Guchte, A., Boyd JM.  Cellular respiration as a trigger for multicellular behavior in Staphylococcus aureus.  Meeting of the New Jersey Antimicrobial Resistance Working Group. Piscataway, NJ  2015.

Poster. Mashruwala A.A., van de Guchte*, A., Roberts C., Eveleigh D.E., Boyd JM, Microbes, Miracles, Medicine—A history of antibiotics at Rutgers.  Meeting of the New Jersey Antimicrobial Resistance Working Group. Piscataway, NJ  2015.

Poster. Bernhardt, C., and Boyd JM. investigating of how Zinc and Tin inhibit Streptococcus mutans.  Aresty Undergraduate Research Symposium.  New Brunswick, NJ 2016.

Poster. Mashruwala A., Earle,C., van de Guchte A., and Boyd JM.  Regulation of Clp proteases by SrrAB in Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ  2016.

Poster. Roberts C., Jasim H., Mashruwala A.A., Rosario-Cruz Z*. Sause W., Torres V., Boyd JM.  The Suf iron-sulfur cluster biosynthetic system is essential for Staphylococcus aureus viability and decreased Suf function results in global metabolic defects and decreased survival in human neutrophils. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ  2016.

Poster.  Al-Tameemi, H.M.*, Mashruwala A.A., Tanner A.W., Carabetta, V.J., Dubnau, D., Boyd J.M.  The YaaT, YlbF, and YmcA proteins are necessary for sporulation in Bacillus subtilis, but what are their functions in the non-sporulating bacterium Staphylococcus aureus? New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ  2016.

Poster. Mashruwala A., Earle,C., van de Guchte A., and Boyd JM.  Regulation of Clp proteases by SrrAB in Staphylococcus aureus.  Joint molecular biosciences graduate student association meeting.  Rutgers University. 2016

Poster. Roberts C., Jasim H., Mashruwala A.A., Rosario-Cruz Z*. Sause W., Torres V., Boyd JM.  The Suf iron-sulfur cluster biosynthetic system is essential for Staphylococcus aureus viability and decreased Suf function results in global metabolic defects and decreased survival in human neutrophils. Joint molecular biosciences graduate student association meeting.  Rutgers University. 2016

Poster.  Al-Tameemi, H.M., Mashruwala A.A., Tanner A.W., Carabetta, V.J.,  Dubnau, D., Boyd JM.  The YaaT, YlbF, and YmcA proteins are necessary for sporulation in Bacillus subtilis, but what are their functions in the non-sporulating bacterium Staphylococcus aureus? Joint molecular biosciences graduate student association meeting.  Rutgers University. 2016

Poster.  Rosario-Cruz Z* Liu G., Montelione G., Boyd JM. The ACME Encoded copBcbl operon protects Staphylococcus aureus from copper intoxication: Cbl is an extracellular membrane-associated copper-binding protein. Joint molecular biosciences graduate student association meeting.  Rutgers University. 2016.

Poster. Mashruwala A., Earle,C.*, van de Guchte A., and Boyd JM.  Regulation of Clp proteases by SrrAB in Staphylococcus aureus. Rutgers Microbiology symposium. Rutgers University 2016

Poster.  Rosario-Cruz Z., Liu G., Montelione G., Boyd JM. The ACME Encoded copBcbl operon protects Staphylococcus aureus from copper intoxication: Cbl is an extracellular membrane-associated copper-binding protein. Rutgers Microbiology symposium. Rutgers University 2016

Poster.  Al-Tameemi, H.M., Mashruwala A.A., Tanner A.W., Carabetta, V.J., Dubnau, D., Boyd JM.  The YaaT, YlbF, and YmcA proteins are necessary for sporulation in Bacillus subtilis, but what are their functions in the non-sporulating bacterium Staphylococcus aureus? Rutgers Microbiology symposium. Rutgers University 2016

Poster. Roberts C., Jasim H., Mashruwala A.A., Rosario-Cruz Z*. Sause W., Torres V., Boyd JM.  The Suf iron-sulfur cluster biosynthetic system is essential for Staphylococcus aureus viability and decreased Suf function results in global metabolic defects and decreased survival in human neutrophils. Rutgers Microbiology symposium. Rutgers University 2016

Poster.  Mashruwala A.A., Bhatt S., Boyd JM. The Duf59 containing protein SufT is required for the maturation of iron-sulfur (FeS) proteins during condictions of high FeS cofactor demand in Staphylococcus aureus.  Rutgers Microbiology symposium. Rutgers University 2016.

Cornell University (New York):

Scillieri Smith J., Moroni P., Santisteban C., Rauch B., Warner B., and Nydam D. Lactococcus and other organisms we are calling “Other Streptococci”: an investigation in Northern New York. National Mastitis Council Regional Meeting pp.10-16. July 28-29, 2015, Syracuse, New York.

Barberio A., Natale A., Ceglie L., Guerrini E., Zuliani F., Lucchese L., Capello K.,  and Moroni P. Patterns of Coxiella burnetii shedding in cow and goat milk. Proceeding of the 54^nd  National Mastitis Council Annual Meeting pp.187-188. February 1-3, 2015, Menphis, Tennesse.

Utah State University:

Kelly EJ, Wilson DJ: Pseudomonas aeruginosa mastitis in two goats associated with contaminated essential-oil based teat dip.  Am Assoc Vet Lab Diag, Greensboro, NC, 2016.

University of Wisconsin:

Fuenzalida, M. J., C. Baumberger, and P. L. Ruegg. 2016. Preliminary results of clinical outcomes of treated and non-treated culture negative cases of clinical mastitis. Poster presentation at Wisconsin Alumni Research Foundation, Discovery Challenge, Univ. Wisconsin, Madison

International:

University of Montreal

Aghamohammadi, M., Kelton, D.F., Barkema, H.W.^, Hogeveen, H., Keefe, G.P., Singh, K., Dufour, S., 2016. Mastitis associated economic loss in Canadian dairy farms. In, World buiatric congress, Dublin, Ireland.

Skoulikas, S., Dufour, S., Haine, D., Perreault, J.Y., Roy, J.P., 2016. Early lactation extended therapy against Staphylococcus aureus intramammary infections in heifers: a randomized controlled trial. In, World buiatric congress, Dublin, Ireland.

Krug, C., DeVries, T.J., Morin, P.A., Roy, J.P., Dubuc, J., Dufour, S., 2016. Validation of the algometer for measuring udder discomfort in postpartum lactating dairy cows. In, World buiatric congress, Dublin, Ireland.

Belage, E., Kelton, D.F., Bauman, C., Dufour, S., 2016. National Dairy Study – A Focus On Udder Health And Milking Management on Canadian Farms In, NMC 55th Annual Meeting, Glendale, AZ, USA.

*bold = technical member for the station

^Scientist from an NE-1048 Experimental Research Station