10/01/2004

Publications jointly authored with other stations.

Sargeant, J., and D. R. Smith. 2002. Chapter 15. The epidemiology of Escherichia coli O157:H7. In, Torrence, M.E. and R.E. Isaacson (eds.), Microbial Food Safety in Animal Agriculture: Current Topics. Iowa State University Press, pp. 131-141.


Publications

Berberov, E.M., Y. Zhou, D.H. Francis, M.A. Scott, S.D. Kachman, and R.A. Moxley. 2003. Relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of Escherichia coli that produces multiple enterotoxins. Infection and Immunity (submitted)

Bergner, D., TB Kuhlenschmidt and MS Kuhlenschmidt . Characterization of a Synthetic Receptor Mimetic for Group A Porcine Rotavirus (submitted).

Burkey TE, Dritz SS, Nietfeld JC, Minton JE. Effect of dietary mannanoligosaccharide and sodium chlorate on the growth performance, acute phase response and bacterial shedding of weaned pigs challenged with Salmonella enterica Serotype Typhimurium. J Anim Sci (submitted).

Butler, J.E., P. Weber, M. Sinkora, D. Baker, A. Schoenherr, B. Mayer and D. Francis. 2002. Antibody repertoire development in fetal and neonatal piglets. VIII. Colonization is required for newborn piglets to make serum antibodies to T-independent and type 2 T-independent antigens. J. Immunology. 169: 6822-6830.

Chang, K.O., Y. Kim, K.Y. Green and L.J. Saif. 2002. Cell culture propagation of porcine enteric calicivirus mediated by intestinal contents is dependent on the cyclic AMP signaling pathway. Virology 304:302-310.

Duhamel, G.E., C.J. Stryker, G. Lu, V.J. Wong, and R.P. Tarara. 2003. Colonic spirochetosis of colony-raised rhesus macaques associated with Brachyspira and Helicobacter. Anaerobe 9:45-55.

Guedes, RM.C. and GebhaIt, C.J. 2003. Preparation and characterization of polyclonal and monoclonal antibodies against Lawsonia intracellularis. J. Vet. Diag. Investi. 15:438 446.

Guedes, RM.C. and Gebhart, C.J. 2003. Comparison of pure culture of Lawsonia intracellularis and intestinal mucosa homogenate as challenge models for porcine proliferative enteropathy. Vet. Microbiol. 93:159 166

Guedes, RM.C. and Gebhart, C.J. 2003. Onset and duration of fecal shedding, cell mediated and humoral immune responses after challenge with a pathogenic isolate or a commercial vaccine of Lawsonia intracellularis. Vet. Microbiol. 91:135 145.

Guedes, R.M.C., Winkelman, N L., and Gebhart, C.J. 2003. Relationship between the severity of porcme proFferative enteropathy and the infectious dose of Lawsonia intracellualaris. Vet. Rec. 153:432 433.

Guo, M. and L.J. Saif. 2002. Pathogenesis of enteric calicivirus infections. In Viral Gastroenteritis, (U. Desselberg and J. Gray, eds.) Elsevier Science, Amsterdam, The Netherlands, pp. 489-504.

Guo, M., J. Vinje and L.J. Saif. 2002. Caliciviruses and other potential foodborne viruses. In Current Topics in Food Safety in Animal Agriculture, (R. Isaacson and M.E. Torrence, eds.), Iowa State Univ. Press, Ames, Iowa, pp. 333-350.

Johnson, JK, Schmidt, J,Gelberg, HB and Kuhlenschmidt MS. (2004) Microbial Adhesion of Cryptosporidium parvum sporozoites: Purification of an Inhibitory Lipid from Bovine Mucosa. J. Parasitol. (in press)

Khaitsa, M.L, D.R. Smith, J.A. Stoner, A.M. Parkhurst, S. Hinkley, T.J. Klopfenstein, and R.A. Moxley. 2003. Incidence, duration and prevalence of Escherichia coli O157:H7 fecal shedding by feedlot cattle during the feeding period. Journal of Food Protection 66:1972-1977

Maes, R. K., D.L. Grooms, A.G. Wise, C. Han, V. Ciesicki, L.Hanson, M.L. Vickers and R. Holland. 2003. Evaluation of a Group A Rotavirus Assay for On-Site Detection of Bovine Rotavirus. J. Clin. Micro. 41; 290-294

Marsteller T.A., Annbruster, G., Bane, D.P., Gebhart, C.J., et al. 2003. Monitoring the prevalence of Lawsonia intracelluiaris IgG antibodies using serial sampling in grow~mg and breeding swine herds. J. Swine Health and Prod. 11:127 130.

Moreno B, Bailey BN, Luo S, Martin MB, Kuhlenschmidt M, Moreno SN, Docampo R, Oldfield E. (2001) (31)P NMR of apicomplexans and the effects of risedronate on Cryptosporidium parvum growth. Biochem Biophys Res Commun. 284:632-7

Potter, A.A., S. Klashinsky, Y. Li, E. Frey, H. Townsend, D. Rogan, G. Erickson, S. Hinkley, T. Klopfenstein, R.A. Moxley, D.R. Smith, and B.B. Finlay. 2004. Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. Vaccine Vol. 22, issues 3-4 (In press)

Raphael, B.H. and L.A. Joens, 2004. Ferrous lron Uptake if Independent of FeoB in Campylobacter jejuni. Can. J. of Micro. In press.

Ristevski, B., Young, A.J., Dudler, L., Cahill, R.N.P., Kimpton, W., Washington, E., Hay, J.B. (2003) Instantaneous labeling of the total blood leukocyte pool to track the development and migration of rare cell types in vivo. International Immunol. 15(2):159-165.

Secomb, T.W., Konderding, M.A., West, C.A., Su, M., Young, A.J., Mentzer, S.J. (2003) Microangiectasias: Structural regulators of lymphocyte transmigration in inflammatory skin. Proc. Nat. Acad. Sci. 100(12):7231-7234.

Smith, D.R., J. Gray, R. Moxley, S. Younts, M. Blackford, S. Hinkley, L. Hungerford, T. Milton, T. Klopfenstein. 2004. A novel diagnostic strategy to classify feedlot pens by the percentage of cattle shedding Escherichia coli O157:H7. Epidemiology and Infection (in press)

Su, M., West, C.A., Young, A.J., He, C., Konerding, M.A., Mentzer, S.J. (2003) Dynamic deformation of migratory efferent lymph-derived cells "trapped" in the inflammatory microcirculation. J. of Cell. Physiol. 194(1):54-62.
Trask, JR, Kalita, PK, Kuhlenschmidt, MS, Smith, RD and TL Funk. Overland and Near-surface Transport of Cryptosporidium parvum. 2004. Environ. Qual. (in press)

Wise, A.G., S.S. Monroe, L.E. Hanson, D.L. Grooms, D. Sockett and R.K. Maes. 2003. Molecular characterization of noroviruses detected in diarrheic stools of Michigan and Wisconsin dairy calves: Circulation of two distinct subgroups. (submitted).

Young, A.J., Holzgreve, W., Dudler, L., Schoeberlein, A., Surbek, D. (2003) Engraftment of human cord blood-derived stem cells in preimmune ovine fetuses after ultrasound-guided in- utero transplantation. .Am. J. Gynecol. Obstet. (In Press)

Theses

Burkey TE. Effect of dietary mannanoligosaccharide and sodium chlorate on the growth performance, acute phase response and bacterial shedding of weaned pigs challenged with Salmonella enterica Serotype Typhimurium. Thesis for Master of Science. Department of Animal Sciences and Industry, Kansas State University, Manhattan, KS 2003.

Brands, D.A. 2003. Thesis. The prevalence and molecular characterization of Salmonella spp. in oysters. University of Arizona, Tucson.

Lee, M.K. 2003. Thesis. Presence, potential virulence and antibiotic susceptibility of Campylobacter jejuni isolated from food and companion animals. University of Arizona, Tucson.

Pitts, T.M. 2001. Thesis. Intra-macrophage survival of Carn~ylobacter jejuni and maturation of C. jejuni containing phagosomes. University of Arizona, Tucson.

Raphael, B.H.. 2002 Thesis. Iron uptake systems of Campylobacter jejuni. University of Arizona, Tucson.

Zhang, R., MS Thesis, 2003, University of Nebraska-Lincoln, Comparative analysis of membrane proteins of human and animal Brachyspira species.


Book Chapters:

Moxley, R.A. 2003. Detection and diagnosis of Escherichia coli O157:H7 in food-producing animals. In, Torrence, M.E. and R.E. Isaacson (eds.), Microbial Food Safety in Animal Agriculture: Current Topics. Iowa State University Press, pp. 143-154.


Abstracts/Proceedings:

Al Ghamdi, G.M., R.M.C. Guedes, T.R Ames, and C.J. Gebhart. 2002. Reproduction of proliferative enteropathy in foals after challenge with Lawsonia intracellularis infected porcine mtestinal mucosa homogenate. Proc. 83rd Conf. Res. Workers An. Dis., St. Louis, MO, November 10 12
Alsop, J., J. Harding, V. Costantini, P. Lewis and L.J. Saif. A trial to determine true TGE status in a multiplier herd. Int. Pig Vet. Symposium, Ames, IA, June 2-5, 2002.

Baker, D.R., R.A. Moxley and D.H. Francis. 2003. Amount of Shiga toxin type 2 (Stx2) expressed by Escherichia coli O157:H7 strains in vitro is correlated with virulence in the gnotobiotic piglet model. Proceedings, 84 Annual Meeting of the Conference of Research Workers in Animal Diseases. Chicago, IL.
Beckler, D.C., C. Mahlum Wees, RM.C. Guedes, and C.J. Gebhart. 2003. Development and comparison of tests for detecting fecal shedding of Lawsonia intracellularis. 34th Annual Mtg. Am. Assoc. Swine Veterinarians, March 8 11, Orlando, FL.

Carvajal, A., M.L. De Arriba, H. Rodriguez, A.B. Vidal, G.E. Duhamel, and P. Rubio. 2003. Prevalence of Brachyspira hyodysenteriae and B. pilosicoli infections among spanish swine herds with diarrhoea. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 43.

Chang, K-O., S. Sosnovtsev, G. Belliot, L.J. Saif, and K.Y. Green. 2003. Generation and characterization of infectious RNA transcripts of porcine enteric calicivirus in cell culture. 22nd Annual Meeting of the Conference of American Society for Virology, Davis, CA, Abst. July 12-16, 2003.

Costantini, B., F. Loisy, J. Joens, F. LeGuyader and L.J. Saif. Calicivirus survey in U.S. Market oysters: preliminary findings. 22nd Annual Meeting of the Conference of American Society for Virology, Davis, CA, Abst. July 12-16, 2003.

Dassanayake, R.P., N.E. Caceres, G. Sarath, and G.E. Duhamel. 2003. Biochemical properties of membrane-associated proteases of Brachyspira pilosicoli isolated from humans with intestinal disorders. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 37.

Dassanayake, R.P., G. Sarath, and G.E. Duhamel. 2003. Identification of penicillin-binding proteins (PBPs) in membrane extracts of Brachyspira pilosicoli. 84th Annual Meeting Conference Research Workers in Animal Diseases, St. Louis, Missouri, November 9-11, P (Poster).

Duhamel, G.E. 2003. Diagnostic procedures for colonic spirochaetal infections, where have we been and where are we going? 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p.13.

Duhamel, G.E., and K. Tarasiuk. 2003. Phenotypic and genotypic characterization of Brachyspira pilosicoli isolated from humans with intestinal disorders. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 27.

Duhamel, G.E., T.K. Jensen, M. Boye, and K. Møller. 2003. Comparative pattern of spirochetal colonization in naturally-occurring swine dysentery and porcine colonic spirochetosis. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 48.

Duhamel, G.E., K. Sestak, C.J. Stryker, G. Lu, and A.A. Lackner. 2003. Colonic spirochetosis of colony-raised rhesus macaques is a polymicrobial disease associated with multiple species of Brachyspira and Helicobacter. 84th Annual Meeting Conference Research Workers in Animal Diseases, St. Louis, Missouri, November 9-11, O (Oral).

Duhamel, G.E., C. Fellström, C.J. Stryker, M. Alexander, A. Gunnarsson, and G. Osterhout. 2003. Characterization of canine Brachyspira by cellular fatty acid analysis and pulse-field gel electrophoresis. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 34.

Duhamel, G.E., C.J. Stryker, and Y.J. Zhou. 2003. Demonstration of Brachyspira pilosicoli association with canine colonic spirochetosis and B. canis as a non-pathogenic commensal. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 20.

Duhamel, G.E., L. Ganley, B.C. Barr, J.P. Whipple, R.W. Nordhausen, R.L. Walker, and H.J. Van Kruiningen. 2003. Colonic spirochetosis of North American opossums (Didelphis virginiana): A potential reservoir of infection for humans and animals. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 42.

Fegan N, Higgs GM, Vanderlinde P, Desmarchelier P, Smith DR. 2003. Pen test devices for detection of E. coli O157 and Salmonella in cattle. VTEC 2003. Edinburgh, Scotland, UK. June 8-11, 2003. Poster

Folmer, J., C. Macken, R. Moxley, D. Smith, M. Brashears, S. Hinkley, G. Erickson, and T. Klopfenstein. 2003. Intervention strategies for reduction of E. coli O157:H7 in feedlot steers. 2003 Nebraska Beef Report. Agricultural Research Division, University of Nebraska Cooperative Extension, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, pp. 22-23, http://animalscience.unl.edu/beef/beef.htm.

Gebhart, C J., Winkolmm, N, Deen, J , and Gramm~ B. 2003. Development of mmmity to Lawsonia intracellulans in pigs fed carbadox. 34th Aunual Mtg. Am. Assoc. Swine Veterinarians, March 8 11, Orlando, FL.

Gebhart, C.J., L.L. Li, Q. Zhang, L.L. Heron, and V. Kapur. 2002. A genomic sequence survey of Lawsonia intracellularis identifies numerous genes of relevance to diagnosis, virulence, and immunoprophylaxis. Proc. 83rd Conf. Res. Workers An. Dis., St. Louis, MO, November 10 12.

Graybill, M.D., Elmubaruk, G., DeRycke, M.S., Young, A.J. 2003. Role of soluble CD14 in the regulation of neonatal ovine B cell differentiation and expression. Proceedings, Autumn Immunology Conference. Chicago, IL

Guedes, RM.C. and C.J. Gebhart. 2003. Progression of Lawsonia intracellularis infection and mucosa immune response in pigs. 34th Annual Mtg. Am. Assoc. Swine Veterinarians, March 8 11, Orlando, FL.

Guedes, R.M.C., C. J. Gebhart, and G.A. Armbruster. 2002. Progression of Lawsonia intracellularis infection in experimentally infected pigs. Proc. 83rd Conf. Res. Workers An. Dis., St. Louis, MO, November 10 12.

Guedes, R.M.C. and C.J. Gebhart. 2002. Production and characterization of new polyclonal and monoclonal antibodies for Lawsonia intracellularis. 29th Allen D. Leman Swine Conference, Minneapolis, MN, September 15 17.

Gwyn, C.B., and G.E. Duhamel. 2003. Porcine colonic spirochetosis caused by Brachyspira pilosicoli. Swine Health Symposium, Stratford, Ontario, Canada, May 16-18, p. 5-12.

Han, M.G., Q. Wang, J.R. Smiley and L.J. Saif. 2003. Self-assembly of recombinant capsid proteins of a bovine norovirus into virus-like particle (VLP). 22nd Annual Meeting of the Conference of American Society for Virology, Davis, CA, Abst. July 12-16, 2003

Hoet, A.E., S. Sreevatsan and L.J. Saif. Molecular analysis of the 5' end of the spike gene of bovine torovirus (breda virus) field strains. Am. Soc. Virol., Lexington, KY, July 20-24, 2002.
Johansson, K.E., G.E. Duhamel, B. Bergsjö, E.E. Olsson, M. Persson, B. Pettersson, and C. Fellström. 2003. Phylogeny of canine intestinal spirochetes based on 16S rDNA analysis. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 33.

Kinsley, K., C. J. Gebhart, N. WinkeEnan, and R.M.C. Guedes. 2003. Passive immunization of hamsters using hyperimmunized chicken eggs to control Lawsonia intracellularis infection: a model for growing swine. 34th Aonual Mtg. Am. Assoc. Swine Veterinarians, March 8 11, Orlando, FL.

Kolappaswamy, K., Y.J. Zhou, and G.E. Duhamel. 2003. Attachment of Brachyspira pilosicoli to cultured intestinal epithelial cell lines. 34th Midwest Student Biomedical Research Forum, Omaha, Nebraska, February 21-22, P-14 (Poster).

Moxley1, R.A., D. R. Smith1, T. J. Klopfenstein2, J. D. Folmer2, C. N. Macken2, G. E. Erickson2, S. Hinkley1, A. A. Potter3, and B. B. Finlay4. 2003. Vaccination and direct-fed microbials as intervention strategies to reduce the prevalence of Escherichia coli O157:H7 in feedlot cattle. 1Dept. of Vet. & Biomed. Sci., 2Dept. of Animal Sci., U. of Nebraska, Lincoln, NE. 3Vaccine & Infectious Disease Org., U. of Saskatchewan, Saskatoon, Saskatchewan, Canada. 4Biotechnology Laboratory, U. of British Columbia, Vancouver, BC, Canada. 84th Annual Meeting Conference Research Workers in Animal Diseases, Chicago, Illinois, Nov. 9-11. Abstract 82 (Oral).

Moxley, R.A. 2003. Escherichia coli O157:H7: an update on intestinal colonization and virulence mechanisms. Dept. of Vet. & Biomed. Sci., U. of Nebraska, Lincoln, NE. 84th Annual Meeting Conference Research Workers in Animal Diseases, Chicago, Illinois, Nov. 9-11. Abstract 92 (Oral, keynote).

Nielsen, P.R., Y. Kim, K.O. Chang and L.J. Saif. Passive protection of neonatal calves against challenge with bovine rotavirus by colostrum supplements from cows vaccinated with a combination of bovine rotavirus-like particles against one or both prevalent U.S. serotypes. Conference for Research Workers in Animal Disease. St. Louis, MO, November 10-12, 2002.

Rice MA, Fang Y, Ambagala APW, Ambagala TC, Srikumaran S, Nelson EA, Duhamel GE. 2003.Characterization of monoclonal antibodies specific for linear B-cell epitopes located within bovine group A rotavirus VP5* . 84th Annual Meeting Conference Research Workers in Animal Diseases, St. Louis, Missouri, November 9-11, P (Poster).

Rohlik, A., DeRycke, M., Young, Alan J. 2003. Identification and Characterization Of Leukocyte Populations In Hoofstock; A tool for the study of Chronic Wasting Disease (CWD) Proceedings, Autumn Immunology Conference. Chicago, IL
Ruesch, L.L., R.L. VanderVeen and D.H. Francis. 2003. Enteroaggregative Escherichia coli heat-stable enterotoxin 1 (EAST1) and its occurrence in enterotoxigenic E. coli strains causing diarrheal disease. Proceedings, 84 Annual Meeting of the Conference of Research Workers in Animal Diseases. Chicago, IL.

Saif, L.J., M. Guo and J. Smiley. Enteric caliciviruses in pigs and calves and their relationship to human caliciviruses. Brazil Soc. Virol., Caldas Novas, Goias, Brazil, Nov. 25-28, 2001.
Saif, L.J. 2002. Coronaviruses: Update on diagnosis, pathogenesis and control of bovine coronavirus-associated calf diarrhea, winter dysentery and shipping fever. Abst. Iowa Veterinary Medical Association Meeting, September 12, 2002, Ames, Iowa.
Smiley, J.R., K.O. Chang, and L.J. Saif. Complete genome sequence of a bovine enteric calicivirus genogroup. Conference of Research Workers in Animal Disease, Abst. #117, St. Louis, MO, Nov. 12-13, 2001.
Smiley, J., K. Chang, J. Vinje and L.J. Saif. Genomic characterization of the enteropathogenic bovine caliciviruses: CV95/OH, CV186/OH and NB strain-a prototype virus of a new calicivirus genogroup. Am. Soc. Virol., Lexington, KY, July 20-24, 2002.
Smith DR. 2003. Feedlot epidemiology of E. coli O157H7 bridging the gaps. Second Governor's Conference on Escherichia coli. Lincoln, NE. April 7-8, 2003. Oral.

Thomas, C., A.E. Hoet, J. Smiley, P. R. Nielsen, T. Wittum and L.J. Saif. Association of bovine torovirus with veal calf diarrhea. Conference for Research Workers in Animal Disease. St. Louis, MO, November 10-12, 2002.

Trask, JR, Kalita, PK, Kuhlenschmidt, MS, Smith, RD and TL Funk. Overland and Near-surface Transport of Cryptosporidium parvum. 2001. Annual American Society For Agricultural Engineering, Paper Number 01-2104.

Wang, Q.H., M.G. Han, A.E. Hoet, and L.J. Saif. Hemagglutination by Cowden porcine enteric calicivirus and development of hemagglutination-inhibition test. Conference for Research Workers in Animal Disease. St. Louis, MO, November 10-12, 2002.

Wang Q.H., M. Guo and L. J. Saif. 2003. Serum neutralizing and isotype antibody responses in gnotobiotic (Gn) pigs inoculated orally with tissue culture-adapted (TC) or wild type (WT) porcine enteric calicivirus (PEC). 22nd Annual Meeting of the Conference of American Society for Virology, Davis, CA, Abst. July 12-16, 2003.

Ward, J.J., RD. Smith, MS Kuhlenschmidt. 2002. Evaluation of Factors Contributing to Environmental Contamination with Cryptosporidium parvum from a Dairy Herd. International Conference on Emerging Infectious Diseases, March 2002, Atlanta, Ga.

Whitney, M.H., G.C. Shurson, R.M.C. Guedes, and C.J. Gebhart. 2002. The relationship between distiller's dried grains with solubles (DDGS) and ileitis. Min. Nutrition Conf ~ Pre Conf Symposium: Rendering a foundation for food security. Eagan, MN, p.244 256.


Winkelman, N., Gebhart, C.J., Wolff, T., and Skinner, J. 2003. An evaluation of BMD plus Aureomycm chlortetracycline (CTC), Tylan or Lmcomix for control of challenge induced PPE m swine. 34th Annual Mtg. Am. Assoc. Swme Veterioarians, March 8 11, Orlando, FL.

Winkehman, N., Gebhart, C.J., Deen, J., and Gramm, B. 2003. Correlation of clinical signs and diagnostic indicators of ileitis in pigs fed carbadox. 34th Aunual Mtg. Am. Assoc. Swine Veterinarians, March 8 11, Orlando, FL
Wise, A. G., L. Hanson, D. Grooms and R. Maes. 2002. Detection and molecular
characterization of bovine Norwalk-like viruses in diarrheie stools collected from Michigan and Wisconsin dairy calves. Proc. of the American Association of Veterinary Laboratory Diagnosticians 45th Annual Meeting, St. Louis, MO 2002. p. 9.

Young, A.J., Richt, J., Hamir, A. 2003. A potential role for B cell subsets in the pathogenesis of Prion Disease. Proceedings, 84 Annual Meeting of the Conference of Research Workers in Animal Diseases. Chicago, IL.

Zhang, W., J. Freeling, E. Berberov, R. Moxley and D. Francis. 2003. Development of a model for the assessment of the contributions of E.coli enterotoxins to the pathogenesis of porcine colibacillosis. Proceedings, 84 Annual Meeting of the Conference of Research Workers in Animal Diseases. Chicago, IL.

Zhang, R., and G.E. Duhamel. 2003. Comparative analysis of pathogenic and commensal porcine Brachyspira species membrane proteins. 2nd International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, United Kingdom, April 2-4, p. 46.

02/09/2005

Arizona

Konkel, M.E. and L.A. Joens: Campylobacter : Molecular and Cellular Biology. Chapter 22. Horizon Press. 2004.

Konkel, M.E., Monteville, M.R., Klena, J.D., Joens, L.A.: Microbial Foiod Safety in Animal Agriculture: Current Topics by M.E. Torrence and R.E. Isaacson. Chapter 21, Iowa Atate Press. June, 2003.

Lee, M.K., S.J. Billington, L.A. Joens. Potential Virulence and Antimicrobial Resistance in Campylobacter jejuni Isolates Obtained from Food and Companion Animals. In Press. Foodborne Path. Dis. 2004.

Raphael, B.H. and L.A. Joens, 2004. Ferrous Iron Uptake is independent of FeoB in Campylobacter jejuni. Can. J. of Micro. In Press.

Brands, D.A. A. Inman, C.H. Gerba, J.Mare, S.J. Billington, L.Saif, J.Levine, and L.A. Joens. 2004. The Prevalence of Salmonella spp. In United States oysters. In Press. App. Envir. Micro 2005.

Brands, D.A., S.J. Billington, J.F. Levine, and L.A. Joens. Genotypes and antibiotic Resistance of Salmonella Newport Isolates from U.S. Market Oysters. In Press Foodborne Path. Dis. 2005


Illinois

J Trask, S. McLaughlin, MS Kuhlenschmidt and P. Kalita 2004 Overland and Near-surface Transport of Cryptosporidium parvum. Environ. Qual. 33:984-983.

Johnson, JK, Schmidt, Gelberg, HB, and Kuhlenschmidt MS. 2004. Microbial Adhesion of Cryptosporidium parvum sporozoites: Purification of an Inhibitory Lipid from Bovine Mucosa. J. Parasitology 90:980-990.


Kansas

Barkey, T.I., Dritz, S.S., Nietfeld, J.C., Johnson, B.J., Minton, J.E. 2004. Effect of dietary nannoligosaccharide and sodium chlorate on the growth performance, acute-phase response, and shedding of weaned pigs challenged with Salmonella enteric serotype Typhimurium. J Anim. Sci. 2004;82:397-404.

Minnesota

Gebhart, C.J. and R.M.C Guedes. 2004. Lawsonia intracellularis. In C.L. Gyles, et al, (ed.), Pathogenesis of Bacterial Infections in Animals, 3rd ed. Blackwell Publishing, Ames, IA.

Walter, D., C.J. Gebhart, J. Kroll, J.T. Holck, and W. Chittick. 2004. Serologic profiling and proper vaccination timing. J. Swine Health Production. (in press).

Guedes, R.M.C. and C. J. Gebhart. 2003. Polyclonal and monoclonal antibodies for diagnosis of proliferative enteropathy. J. Vet. Diag. Investi. 15:438-446.


Nebraska

Berberov, E. M., Y. Zhou, D. H. Francis, M. A. Scott, S. D. Kachman, and R. A. Moxley. 2004. Relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of Escherichia coli that produces multiple enterotoxins. Infect. Immun. 72:3914-3924.

Dassanayake, R. P., N. E. Caceres, G. Sarath, and G. E. Duhamel. 2004. Biochemical properties of membrane-associated proteases of Brachyspira pilosicoli isolated from humans with intestinal disorders. J. Med. Microbiol. 53:319-323.

Dassanayake, R. P., Y. Zhou, S. Hinkley, C. J. Stryker, G. Plauche, J. T. Borda, K. Sestak, and G. E. Duhamel. Characterization of cytolethal distending toxin of Campylobacter species isolated from captive macaque monkeys. J. Clin. Microbiol., in press.

De Cock, H. E. V., S. L. Marks, B. A. Stacy, T. Zabka, J. Burkitt, G. Lu, D. J. Steffen, and G. E. Duhamel. 2004. Ileocolitis associated with Anaerobiospirillum in cats. J. Clin. Microbiol. 42:2752-2758.

Folmer, J. D., C. N. Macken, R. A. Moxley, D. R. Smith, S. Hinkley, G. E. Erickson, A. A. Potter, B. B. Finlay, and T. J. Klopfenstein. 2004. Vaccination and direct-fed microbials as intervention strategies for reduction of E. coli O157:H7 in feedlot steers. 2004 Nebraska Beef Cattle Report, University of Nebraska Cooperative Extension MP 80-A, pp. 68-71.

Johansson, K. E., G. E. Duhamel, B. Bergsjö, E. O. Engvall, M. Persson, B. Pettersson, and C. Fellström. 2004. Identification of three clusters of canine intestinal spirochaetes by biochemical and 16S rDNA sequence analysis. J. Med. Microbiol. 53:345-350.

Moxley, R. A. 2004. Escherichia coli O157:H7: an update on intestinal colonization and virulence mechanisms. Anim. Health Res. Rev. 5:15-33.

Potter, A. A., S. Klashinsky, Y. Li, E. Frey, H. Townsend, D. Rogan, G. Erickson, S. Hinkley, T. Klopfenstein, R. A. Moxley, D. R. Smith, and B. B. Finlay. 2004. Decreased shedding of Escherichia coli O157:H7 by cattle following vaccination with type III secreted proteins. Vaccine 22:362-369.

Smith, D. R., J. T. Gray, R. A. Moxley, S. M Younts-Dahl, M. P. Blackford, S. Hinkley, L. Hungerford, C. T. Milton, and T. J. Klopfenstein. 2004. A diagnostic strategy to determine the Shiga toxin-producing Escherichia coli status of pens of feedlot cattle. Epidemiol. Infect. 132:297-302.

Yang, Z., J. Kovar, J. Kim, J. Nietfeldt, D. R. Smith, R. A. Moxley, M. E. Olson, P. D. Fey, and A. K. Benson. 2004. Identification of common subpopulations of non-sorbitol-fermenting, $-glucuronidase-negative Escherichia coli O157:H7 from bovine production environments and human clinical samples. Applied Environ. Microbiol. 70:6846-6854.


Ohio

Chang, K.O., S.V. Sosnovtsev, G. Belliot, Y. Kim, L.J. Saif, K.Y. Green. 2004. Bile acids are essential for porcine enteric calicivirus replication in association with down-regulation of signal transducer and activator of transcription 1. Proc Natl Acad Sci USA. Jun 8; 101(23):8733-8.

Han, M.G., J.R. Smiley, C. Thomas, L.J. Saif. 2004. Genetic Recombination between Two Genotypes of Genogroup III Bovine Noroviruses (BoNVs) and Capsid Sequence Diversity among BoNVs and Nebraska-Like Bovine Enteric Caliciviruses. J. Clin. Microbiol. 42: 5214-5224.

Han, M.G., Q. Wang, J.R. Smiley, K.O. Chang, L.J.Saif. 2004. Self-assembly of the recombinant capsid protein of a bovine norovirus (BoNV) into virus-like particles and evaluation of cross-reactivity of BoNV with human noroviruses. J. Clin. Microbiol. (in press).

Saif, L. J. 2004. Bovine Coronavirus Infections. In Infectious Diseases of Livestock, (Coetzer, J.A.W., Thomson, G.R.) Oxford Univ. Press, Oxford, England.

Saif, L.J. 2004. Comparative biology of animal coronaviruses: Lessons for SARS. In: SARS The first new plague of the 21st century (M. Peiris, ed), Blackwell Pub., Oxford, UK (in press).

Saif, L.J. 2004. Animal Coronaviruses: Lessons for SARS. In: Learning from SARS: Preparing for the next disease outbreak. SARS workshop sponsored by the Institute of Medicine of the National Academy of Sciences, Washington, DC, Sept. 30-Oct. 1, 2003. (in press).

Hodgins, D. C., L. Yuan L., V. Parreno, L.B. Corbeil, and L.J. Saif. Mucosal Veterinary Vaccines. In: Mucosal Immunology. Third Edition. Edited by McGhee J. R. Mestecky, J. et al., Academic Press. 2004 (in press).

Yuan, L., G. Stevenson and L.J. Saif. Rotavirus and Reovirus. (2004). In: Diseases of Swine. 9th Edition. Edited by Zimmerman, J. J. et al. Ames, Iowa: Iowa State University Press (in press).

Saif. L.J. 2004. Animal coronaviruses: what can they teach us about the severe acute respiratory syndrome? Rev. Sci. Tech. Off. Int. Epiz., 23(2): (in press).

Saif, L.J. 2004. Animal coronaviruses vaccines: Lessons for SARS. In: Control of Infectious Animal Diseases by Vaccination (Schudel A., M. Lombard, eds). Dev. Biol. Basel, Karger, vol. 119 (in press).


South Dakota

Berberov, E.M, Y. Zhou, D.H. Francis, M.A. Scott, S.D. Kachman and R.A. Moxley. 2004. Relative importance of heat labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of enterotoxigenic Escherichia coli that produces multiple enterotoxins. Infect Immun 72: 3914-3924


Washington

Bae, W, KN Kaya, DD Hancock, DR Call, YH Park, and TE Besser. Accepted. Prevalence and antimicrobial resistance of thermophilic Campylobacter sp. from cattle farms in the Northwestern United States. Applied and Environmental Microbiology.

Borucki, MK, SH Kim, DR Call, S. Smole, and F. Pagotto. Accepted. Discrimination of Listeria monocytogenes epidemic strains using a mixed-genome DNA microarray. Journal of Clinical Microbiology.

Cobbold, R. N. and Desmarchelier, P. 2003. In vitro studies on the colonization of bovine colonic mucosa by Shiga-toxigenic Escherichia coli (STEC). Epidemiology and Infection. 132: 87-94.

Cobbold, R.N.; Rice, D.H.; Szymanski, M.; Call, D. R.; Hancock, D.D.. 2004. Comparison of Shiga-toxigenic Escherichia coli (STEC) prevalence between dairy, feedlot and cow-calf herds in Washington State. Applied and Environmental Microbiology. 70 (7): 4375-4378.

DeFrancesco, K.A.; Cobbold, R.N.; Rice, D.H.; Besser, T.E.; Hancock, D.D.. 2004. Antimicrobial Resistance of Commensal Escherichia coli from Dairy Cattle Associated With Recent Salmonellosis Outbreaks. Veterinary Microbiology. 98 (1): 55-61.

González, SF, MJ Krug, ME Nielson, Y. Santos, and DR Call. 2004. Simultaneous detection of marine fish pathogens using multiplexed PCR and DNA microarrays. Journal of Clinical Microbiology 42:1414-1419.

Khachatryan, AR, DD Hancock, TE Besser, and DR Call. 2004. The role of calf-adapted Escherichia coli in maintenance of antibiotic resistance in dairy calves. Applied and Environmental Microbiology 70:752-757.

Lahmers, S, Y Wu, DR Call, S. Labeit, and H. Granzier. 2004. Developmental control of titin isoform expression and passive stiffness in fetal and neonatal myocardium. Circulation Research 94:505-513.

Lane, S., J. Evermann, F. Loge and D.R. Call. 2004. Secondary structure prevents target hybridization to oligonucleotide microarrays. Biosensors and Bioelectronics.
ORourke KI, Spraker TR, Hamburg LK, Besser TE, Brayton KA, Knowles DP. 2004.

Panicker, G, DR Call, MJ Krug, and AK Bej. Accepted. Detection of pathogenic Vibrio spp. in shellfish and Gulf of Mexico water using multiplex PCR and DNA-array hybridization. Applied and Environmental Microbiology.

Straub, TM, Quinoñez Díaz, MD, CO Valdez, DR Call, and DP Chandler. 2004. Using DNA microarrays to detect multiple pathogen threats in water. Water Science and Technology: Water Supply 4:107-114.

Warsen, A, MJ Krug, S LaFrentz, DR Stanek, FJ Loge, and DR Call. 2004. Simultaneous discrimination between 15 fish pathogens using 16S rDNA PCR and DNA microarrays. Applied and Environmental Microbiology 70:4216-4221.

Willse, A, TM Straub, S. Wunschel, JA Small, DR Call, D Daly, and DP Chandler. 2004. Quantitative oligonucleotide microarray fingerprinting of closely related Salmonella enterica isolates. Nucleic Acid Research 32:1848-1856.

Woodford, NL, DR Call, DG Remick, and R Rochford. 2004. A model of angiogenesis in SCID mice xenoengrafted with Epstein-Barr virus transformed B cells. Comparative Medicine 54:209-215.

10/10/2005

Publications 2005
AZ
Thesis & Dissertations
Law, B.F. Assessment of the Pathogenicity of Campylobacter jejuni from Broiler Chickens. Doctorial Dissertation, University of Arizona, Tucson, AZ. 2005.

Reeser, Ryan. Campylobacter jejuni: Characterization of a microbial Biofilm on Abiotic Surfaces. Masters Thesis, University of Arizona, Tucon, AZ. 2005.

Refereed Publications
Lee, M.K., S.J. Billington, L.A. Joens. Potential Virulence and Antimicrobial Resistance in Campylobacter jejuni Isolates Obtained from Food and Companion Animals. Foodborne Path. Dis. 1:223-230. 2004.

Brands, D.A., A.E. Inman, C.P. Gerba, C.j. Mare, S.J. Billington, L. Saif, J.F. Levine, L.A. Joens. The Prevalence of Salmonella spp. In U.S. oysters. App. Env. Mic. 71:893-897. 2005

Brands, D.A., S.J. Billington, J.F. Levine, L.A. Joens. Genotyping and Resistance Determination of Salmonella Newport Isolates Obtained from U.S. Market Oysters. Foodborne Path. Dis. 2:111-114. 2005.

Book Chapters
Joens, L.A.: Campylobacter: Molecular and Cellular Biology by M.E. Konkel. Chapter 22, p 415-420. Horizon Press. 2004.

IL
Kuhlenschmidt MS, Rolsma MD, Kuhlenschmidt T, Gelberg HB. Characterization of a Porcine Enterocyte Receptor for Group A Rotavirus. In: Mechanisms in the Pathogenesis of Enteric Diseases. ed., Paul PS, Francis DH, Benfield DA., pp. 135-143, Plenum Press, New York, N.Y., 1997.

MD Rolsma, TB Kuhlenschmidt., HB Gelberg and MS Kuhlenschmidt (1998) Structure and Function of a Porcine Enterocyte Ganglioside Receptor for Group A Rotavirus. Journal of Virology 72:9079-9091.

TB Kuhlenschmidt. B Hanafin and MS Kuhlenschmidt (1999) Sialic Acid Dependence and Indpendence of Group A Rotavirus. Adv. In Experimental Biology and Medicine 473:309-317
Gelberg HB, Thulin JD, Kuhlenschmidt MS. Application of Intestinal Xenografts to the Study of Enteropathogenic Infectious Disease. In: Mechanisms in the Pathogenesis of Enteric Diseases. ed., Paul PS, Francis DH, Benfield DA., pp. 31-35, Plenum Press, New York, N.Y., 1997.

Rolsma MD, Kuhlenschmidt TK, Gelberg HB, Kuhlenschmidt MS. 1998 Structure and function of a ganglioside receptor for porcine group A rotavirus. J. Virol. 72:9079-9091)

Moreno B, Bailey BN, Luo S, Martin MB, Kuhlenschmidt M, Moreno SN, Docampo R, Oldfield E. (2001) (31)P NMR of apicomplexans and the effects of risedronate on Cryptosporidium parvum growth. Biochem Biophys Res Commun. 284:632-637.

J Trask, S. McLaughlin, MS Kuhlenschmidt and P. Kalita 2004 Overland and Near-surface Transport of Cryptosporidium parvum. Environ. Qual. 33:984-983.

Johnson, JK, Schmidt, Gelberg, HB, and Kuhlenschmidt MS. 2004. Microbial Adhesion of Cryptosporidium parvum sporozoites: Purification of an Inhibitory Lipid from Bovine Mucosa. J. Parasitology 90:980-990.

Wetzel DM., Schmidt J, Kuhlenschmidt MS., Dubey JP, and Sibley LD. 2005.Gliding motility leads to active cellular invasion by Cryptosporidium parvum sporozoites. Infection and Immunity 73:5379-5387.
Abstracts:

Johnson JK, Kuhlenschmidt MS, Gelberg HB. Glycoconjugates and plasma membrane vesicles inhibit Cryptosporidium parvum sporozoite-host cell recognition. Vet Pathol 34:509, 1997.

JK Johnson, HB Gelberg, and MS Kuhlenschmidt (1999) - Partial Purification of a Cryptosporidium parvum Sporozoite Host Cell Receptor from Plasma Membrane Vesicles. Second Annual Conference on New and Re-Emerging Infectioius Diseases.

JK Johnson, HB Gelberg and MS Kuhlenschmidt (1999) - Partial Purification of a Cell Surface Receptor for Cryptosporidium parvum sporozoite Attachment. Proceedings: American College of Veterinary Pathologists, 50th Annual Meeting, Chicago, IL.

MS Kuhlenschmidt, JK Johnson, and HB Gelberg. 1998. Glycoconjugates and Plasma Membrane Vesicles Inhibit Cryptosporidium parvum Sporozoite-Host Cell Recognition. Second International Rushmore Conference on Mechanisms in the Pathogenesis of Enteric Disease.

JJ Ward, RD Smith, and MS Kuhlenschmidt (1999) Evaluation of the Premier Cryptosporidium by Meridian Diagnostics for the detection of C. parvum in dairy cattle. Conference for Research Workers in Animal Diseases Proceedings.
JJ, Smith RD, and Kuhlenschmidt, MS (1999).Critical control points for reducing environmental contamination with Cryptosporidium parvum oocysts from dairy cattle, Proceedings, 9th Symposium of the International Society for Veterinary Epidemiology and Economics, Aug 6-11, 2000; Breckenridge, CO.
JJ Ward, Smith RD, and Kuhlenschmidt, MS. (2000) Simulation model to predict environmental contamination with Cryptosporidium parvum oocysts from a dairy herd,Proceedings, 81st Annual Meeting of the Conference of Research Workers on Animal Diseases (CRWAD). Nov 12-14, 2000; Chicago, IL.

Trask, J. R., P. K. Kalita, M. S. Kulhenschmidt, R. D. Smith, and T. L. Funk. 2001. Overland and Near-surface Transport of Cryptosporidium parvum. ASAE Annual International Meeting 01-2104: 1-14.

JJ Ward, RD. Smith, MS Kuhlenschmidt. 2002. Evaluation of Factors Contributing to Environmental Contamination with Cryptosporidium parvum from a Dairy Herd. International Conference on Emerging Infectious Diseases, March 2002, Atlanta, Ga.

Salto, M.L., Kuhlenschmidt, T., Kuhlenschmidt, M., de Lederkremer, R. M., and Docampo, R. 2004. Lipid composition of acidocalcisomes of Trypanosoma cruzi. 7th Annual Conference on New and Re-Emerging Infectious Diseases. Urbana, April 15-16, p. 23. http://www.cvm.uiuc.edu/idc/

Ochonicky K, Donovan, S, Kuhlenschmidt T. and Kuhlenschmidt M. 2005. Inhibitory effects of human and porcine milk oligosaccharides on sialic acid dependent and sialic acid independent strains of rotavirus. American Dairy Science Association Annual Meeting, Cincinatti, OH, July 24-28, 2005.

Ochonicky K, Donovan S. Kuhlenschmidt T, Jimenez-Flores R, Kuhlenschmidt M. 2005. Inhibitory activity of bovine milk fat globule membrane against sialic acid-dependent and independent strains of rotavirus American Dairy Science Association Annual Meeting, Cincinatti, OH, July 24-28, 2005.

Andres A., Donovan S.M., Kuhlenschmidt T.B., Kuhlenschmidt M.S. 2005. Isoflavones at concentrations present in soy-based infant formula inhibit rotavirus infectivity in vitro. The FASEB Journal 2005;19: A446.

MN
Book Chapter
S. McOrist and Gebhart, C.J. 2005. Proliferative Enteropathies. In B. Straw, et al, (ed.), Diseases of Swine, Ninth Edition. Blackwell Publishing, Ames, IA.

Journal Article
Wattanaphansak, S., C.J. Gebhart, M. Olin, and J. Deen. 2005. Measurement of the viability of Lawsonia intracellularis. Can. J. Vet. Res. (in press).

Abstracts
Beckler, D.C., V. Kapur and C.J. Gebhart. 2004. Molecular epidemiologic typing of Lawsonia intracellularis. Proc. 85th Conf. Res. Workers An. Dis., Chicago, IL, November 14-16.

Nuntaprasert, A, K. Kaur, C.J. Gebhart and V. Kapur. 2004. Expression and purification of a flagellar protein of Lawsonia intracellularis. Proc. 85th Conf. Res. Workers An. Dis., Chicago, IL, November 14-16.

Wattanaphansak, S. , K. Kinsley, J. Deen and C. Gebhart. 2004. Comparative agreement of indirect fluorescence antibody test and immunoperoxidase monolayer assay for serological diagnosis of field cases of porcine proliferative enteropathy. Proc. 85th Conf. Res. Workers An. Dis., Chicago, IL, November 14-16.

Wattanaphansak, S, C. Gebhart, M. Olin, A. Nuntraprasert and J. Deen. 2004. Measurement of the viability of Lawsonia intracellularis. Proc. 85th Conf. Res. Workers An. Dis., Chicago, IL, November 14-16.

Beckler, D.C., N.L. Weber and C.J. Gebhart. 2005. Typing of Lawsonia intracellularis isolates by analysis of variable number tandem repeat profiles. Proc. Am. Assoc. Swine Pract., Toronto, Canada

Wattanaphansak, S., T. Asawakarn, J. Deen, and C. Gebhart. 2005. Development of an enzyme-linked immunosorbent assay for the diagnosis of porcine proliferative enteropathy. Proc. Allen D. Leman Swine Conf. vol. 32, St. Paul, MN

Asawakarn, T., A. Nuntaprasert, K. Kaur, V. Kapur, S. Wattanaphansak, and C. Gebhart. 2005. Expression of recombinant Lawsonia intracellularis proteins. Proc. Allen D. Leman Swine Conf. vol. 32. , St. Paul, MN

Wattanaphansak, S., T. Asawakarn, J. Deen, and C. Gebhart. 2005. Development of an enzyme-linked immunosorbent assay for the diagnosis of porcine proliferative enteropathy. Rushmore Conference, Sept. 29-Oct.2, Rapid City, SD

Asawakarn, T., A. Nuntaprasert, K. Kaur, V. Kapur, S. Wattanaphansak, and C. Gebhart. 2005. Expression and immunogenicity of proteins encoded by the genome of Lawsonia intracellularis. Rushmore Conference, Sept. 29-Oct.2, Rapid City, SD

NE
Extension Reports:
Peterson, R. E., D. R. Smith, R. A. Moxley, T. J. Klopfenstein, S. Hinkley, and G. E. Erickson. Large-scale clinical trial to evaluate an experimental Escherichia coli vaccine. 2006 Nebraska Beef Report. University of Nebraska Cooperative Extension .

Peterson, R. E., D. R. Smith, R. A. Moxley, T. J. Klopfenstein, S. Hinkley, and G. E. Erickson. Vaccination for Escherichia coli O157:H7 in market ready feedlot cattle. 2006 Nebraska Beef Report. University of Nebraska Cooperative Extension.

Journal Articles:
Carvajal, A., M.L. De Arriba, H. Rodriguez, A.B. Vidal, G.E. Duhamel, and P. Rubio. 2005. Brachyspira hyodysenteriae is relatively more prevalent than B. pilosicoli among commercial pig farms with diarrhoea in Spain. Vet. Rec., in press.

Dassanayake, R.P., M.A. Griep, and G.E. Duhamel. 2005. The cytolethal distending toxin B sub-unit of Helicobacter hepaticus is a Ca2+- and Mg2+-dependent neutral nuclease. FEMS Microbiol Lett 251:219-225.

Dassanayake, R.P., G. Sarath, and G.E. Duhamel. 2005. Penicillin-binding proteins in the pathogenic intestinal spirochete Brachyspira pilosicoli. Antimicrob. Agents Chemother. 49: 1561-1563.

Dassanayake, R.P., Y. Zhou, S. Hinkley, C.J. Stryker, G. Plauche, J.T. Borda, K. Sestak, and G.E. Duhamel. 2005. Characterization of cytolethal distending toxin of Campylobacter species isolated from captive macaque monkeys. J. Clin. Microbiol. 43:641-649.

Smith, D. R., R. A. Moxley, S. L. Clowser, J. D. Folmer, S. Hinkley, G. E. Erickson, and T. J. Klopfenstein. 2005. Use of rope-devices to describe and explain the feedlot ecology of Salmonella by time and place. Foodborne Pathog. Dis. 2:61-69.

Smith, D. R., R. A. Moxley, S. L. Clowser, J. D. Folmer, S. Hinkley, G. E. Erickson, and T. J. Klopfenstein. 2005. Use of rope-devices to describe and explain the feedlot ecology of Escherichia coli O157:H7 by time and place. Foodborne Pathog. Dis. 2:50-60.

Shivaprasad, H.L., and G.E. Duhamel. 2005. Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys. Avian Dis., in press.

Book Chapters:
Hampson, D.J., and G.E. Duhamel. 2005. Porcine colonic spirochetosis/Intestinal spirochetosis. In: Diseases of Swine. 9th ed. Straw BE, DAllaire S, Mengeling WL, and Taylor DJ (eds). Iowa State University Press, Ames, Iowa, pp. 755-767.

Other Presentations and Published Abstracts:
Bretschneider, G., E. M. Berberov, and R. A. Moxley. 2005. Role of the Tir protein in Escherichia coli O157:H7 intestinal colonization of adult cattle. Nebraska Symposium on Interdisciplinary Graduate Science Research, University of Nebraska-Lincoln, Lincoln, NE, Sept. 27 (Poster).

Bretschneider, G., E. M. Berberov, and R. A. Moxley. 2005. Role of the Tir protein in Escherichia coli O157:H7 intestinal colonization of adult cattle. Third International Rushmore Conference on Enteric Diseases, Rapid City, SD, Sept. 29-Oct. 1 (Poster).

Dassanayake, R.P., C.J. Stryker, R.K. Johnson, C.J. Gebhart, K.W. Post, S. Hinkley, W.T. Muraoka, I.V. Wesley, and G.E. Duhamel. 2005. The US porcine Campylobacter coli are negative for cytolethal distending toxin activity. 85th Annual Meeting Conference Research Workers in Animal Diseases, St. Louis, Missouri, November 6-8, (Poster).

Dassanayake, R.P., and G.E. Duhamel. 2005. The cytolethal distending toxin B sub-unit of Helicobacter hepaticus localizes to the nucleus and is sufficient for intoxication of eukaryotic cells. 3rd international Rushmore Conference on Enteric Diseases, Rapid City, South Dakota, September 29 - October 1, (Poster).

Dassanayake, R.P., C.J. Stryker, R.K. Johnson, W.T. Muraoka, I.V. Wesley, and G.E. Duhamel. 2005. Characterization of a novel Campylobacter cytolethal distending toxin from Campylobacter hyointestinalis subsp. hyointestinalis isolated from humans and pigs. 3rd international Rushmore Conference on Enteric Diseases, Rapid City, South Dakota, September 29 - October 1, (Poster).

Dassanayake, R.P., M.A. Griep, and G.E. Duhamel. 2005. The cytolethal distending toxin B subunit of Helicobacter hepaticus is a nuclear localizing Ca2+- and Mg2+-dependent endonuclease. 105th General Meeting of the American Society for Microbiology, Atlanta, Georgia, June 5-9, Abst. B-008, p. (Poster).

Duhamel, G.E. 2005. Efficacy of antimicrobial agents for PCS control. Pig Progress, Enteric Diseases Special III., p. 6-8.

Duhamel, G.E. 2005. Understanding of colitis in swine improved. Section 4 in Perspectives on Swine Disease Management, Novartis Animal Health, Basel, Switzerland, p. 1-6.

Duhamel, G.E. 2005. In vitro and in vivo efficacy of antimicrobial agents for control of porcine colonic spirochaetosis. Section 5 in Perspectives on Swine Disease Management, Novartis Animal Health, Basel, Switzerland, p. 1-6.

Erume, J., E. M. Berberov, and R. A. Moxley. 2005. Disruption of enterotoxin genes of enterotoxigenic Escherichia coli by allelic exchange using lambda Red-mediated recombineering. Nebraska Symposium on Interdisciplinary Graduate Science Research, University of Nebraska-Lincoln, Lincoln, NE, Sept. 27 (Oral).

Erume, J., E. M. Berberov, and R. A. Moxley. 2005. Disruption of enterotoxin genes of enterotoxigenic Escherichia coli by allelic exchange using lambda Red-mediated recombineering. Third International Rushmore Conference on Enteric Diseases, Rapid City, SD, Sept. 29-Oct. 1 (Poster).

Smith DR. 2005. The future of E. coli O157:H7 intervention in live cattle. Cardinal Meats Conference on Food Safety. Toronto, Ontario, Canada. June 24, 2005 (Oral)

Smith DR. 2005. Can vaccination reduce the probability that feedlot cattle shed Escherichia coli O157:H7? Invited seminar, University of Guelph, Ontario Veterinary College. Guelph, Ontario, Canada. Apr 29, 2005 (Oral)

Smith DR, Moxley RA, Klopfenstein TJ, Peterson RE, Erickson GE. 2005. Population-based strategies for monitoring food safety pathogens in feedlot cattle. Beef Industry Food Safety Council (BIFSCO). Orlando, FL. April 20, 2005. (Oral)

Smith DR. 2005. Can vaccination reduce the probability that feedlot cattle shed Escherichia coli O157:H7? Beef Industry Food Safety Summit. Orlando, FL. April 19, 2005. (Oral)

Smith DR. 2005. Multidrug-resistant Salmonella: the bovine practitioners role in public health. National Conference on Ground Beef Contaminated with Multidrug-Resistant Salmonella, Including S. Typhimurium DT104: An Emerging Public Health Concern. Tufts University School of Veterinary Medicine, Grafton, MA. March 7-8, 2005. (Oral)

Smith DR. 2005. Preventing calf scours with the Sandhills Calving System. Cattlemens Seedstock Showcase. Phillipsburg, KS. Feb 7, 2005. (Oral)

Smith DR. 2005. Preventing calf scours with the Sandhills Calving System. UNL Gudmundsen Sandhills Laboratory, Whitman, NE. Jan 4, 2005 (Oral)

Smith DR. 2005. Preventing calf scours with the Sandhills Calving System. UNL Extension, Holt County, ONeill, NE. Feb 18, 2005. (Oral)

Smith DR. 2005. Preventing calf scours with the Sandhills Calving System. UNL Extension, Brown, Rock, KeyaPaha Counties, Ainsworth, NE. Feb 17, 2005. (Oral)

Smith DR. 2005. Preventing calf scours with the Sandhills Calving System. UNL Extension, Custer County. Broken Bow, NE Feb 3, 2005. (Oral)

Smith DR. 2005. Preventing calf scours with the Sandhills Calving System. Montrose /Dell Rapids Veterinary Clinic Client Education Meeting, Dell Rapids, SD, Jan 27, 2005. (Oral)

OH
Book Chapters

1. Saif, L.J. 2005. Comparative biology of animal coronaviruses: Lessons for SARS. In: Severe Acute Respiratory Syndrome. (M. Peiris, et al ed), Blackwell Pub., Oxford, UK, pp. 84-99.
2. Saif, L.J. 2004. Animal Coronaviruses: Lessons for SARS. In: Learning from SARS: Preparing for the next disease outbreak. SARS workshop sponsored by the Institute of Medicine of the National Academy of Sciences, Washington, DC, Sept. 30-Oct. 1, 2003, pp 138-148.
3. Yuan, L., G. Stevenson and L.J. Saif. 2005. Rotavirus and Reovirus. In: Diseases of Swine. 9th Edition. Edited by Zimmerman, J. J. et al. Ames, Iowa: Iowa State University Press (in press).
4. Saif. L.J. and K. Sestak. 2005. TGEV and PRCV. In: Diseases of Swine. 9th Edition. Edited by Zimmerman, J. J. et al. Ames, Iowa: Iowa State University Press (in press).
5. Costantini, V. and L.J. Saif. 2006. Calicivirus and Rotaviruses. In: Fate and transport of zoonotic bacterial, viral and protozoan pathogens during swine manure storage, treatment, and land application, Council for Agricultural Science & Technology, NPB, (submitted).

Refereed Journal Articles
1. Chang, K. O., S. S. Sosnovtsev, G. Belliot, Q. Wang, L. J. Saif, and K. Y. Green. 2005. Reverse genetics system for porcine enteric calicivirus, a prototype sapovirus in the Caliciviridae. J Virol 79:1409-16.
2. Wang, Q., M. G. Han, S. Cheetham, M. Souza, J. A. Funk, and L. J. Saif. 2005. Porcine noroviruses: genetic and antigenic relationships to human noroviruses. Emerg Infect Dis.(in press).
3. Wang, Q., M. K. Han, J. A. Funk, G. Bowman, and L. J. Saif. 2005. Genetic diversity and recombination of porcine sapoviruses. J Clin Microbiol. (in press).
4. Wang, Q., K. O. Chang, M. K. Han, S. Sreevatsan, and L. J. Saif. 2005. Development of a new microwell hybridization assay and an internal control RNA for the detection of porcine noroviruses and sapoviruses by reverse transcription-PCR. J Virol Methods. (in press).
5. Han, M.G., S. Cheetham, M. Azevedo, C, Thomas, and L. J. Saif. 2005. Immune responses to bovine norovirus-like particles with various adjuvants and analysis of protection in gnotobiotic calves. Vaccine. (in press).
6. Thomas, C., A. Hoet, S. Sreevatsan, T. Wittum, R. Briggs, G. Duff and L.J. Saif. 2005. Field transmission of bovine coronavirus and herd immunity against associated respiratory disease in feedlot cattle. AJVR (submitted).
7. Costantini, V., F. Loisy, L. Joens, F.S. LeGuyader and L.J. Saif. 2005. Human and animal enteric caliciviruses in oysters from different coastal regions of the U.S. Appl. Envir. Microbiol. (submitted).

Abstracts
1. Wang, Q., M. G. Han, S. Cheetham, M. Souza, J. Funk and L. J. Saif. 2005. Detection of porcine noroviruses (PoNoV) from US swine and their genetic and antigenic relationships to human noroviruses (HuNoV). To be presented at the 86th Conference of Research Workers in Animal Diseases, St. Louis, Missouri, December 4-6, 2005.
2. Costantini, V., F. Loisy, J. Joens, F. LeGuyader and L.J. Saif. 2005. Update on calicivirus survey of U.S. market oysters: animal enteric calicivirus. 24th Annual Meeting of the Conference of American Society for Virology, University Park, Pennsylvania, U.S. Abst. #50-10. June 18-22, 2005.

Thesis
Thomas, C. 2005. Bovine enteric and respiratory viruses: Studies of bovine enteric calicivirus and bovine coronavirus. MS Thesis, The Ohio State University.

SD
Butler, J. E., D. Francis, J. Freeling, P. Weber, J. Sun, K. L. Nielsen and A. M. Krieg. 2005. Antibody repertoire development in fetal and neonatal piglets. IX. Three PAMPs act synergistically to allow germfree piglets to respond to TI-2 and TD antigens. J. Immunol. 175: 6772-6785.
Boots, RE, DH Francis and J. Freeling. Factors Influencing F18+ Escherichia coli Receptor Expression in Weanling Pigs. Abstract. Third International Rushmore Conference on Enteric Diseases. Rapid City, SD.
Christie A., V Sebastien, S Gibson, D Francis and V Brozel. 2005. Proteomic analysis of Escherichia coli O157:H7 growing in soil organic matter reveals a unique phenotype. Abstract. Third International Rushmore Conference on Enteric Diseases. Rapid City, SD.
Francis D, A Erickson, R Moxley, W Zhang and E Berberov. 2005. Use of germ-free pigs in modeling enterotoxigenic E. coli infections. Invited Presentation. Third International Rushmore Conference on Enteric Diseases. Rapid City, SD.
Kaushik R, S George, A Young, and D Francis. 2005. Lectin binding profile of porcine intestinal epithelial cell lines. Abstract. Conference of Research Workers in Animal Diseases, Annual Mtg. St Louis MO.
Kaushik RS, S George, A Young, and D Francis. 2005. Lectin binding profile of porcine intestinal epithelial cell lines. Abstract. Third International Rushmore Conference on Enteric Diseases. Rapid City, SD.
Koh S, S George, V Brözel, D Francis and R Kaushik. 2005. Porcine Intestinal Epithelial Cell Lines as an in vitro Model for Studying Pathogenesis of Enterotoxigenic Escherichia coli. Abstract. Conference of Research Workers in Animal Diseases, Annual Mtg. St Louis MO.
Koh SY, S George, V Brozel, D Francis and RS Kaushik. 2005. Porcine intestinal epithelial cell lines as an in vitro model for studying pathogenesis of enterotoxigenic Escherichia coli. Abstract. Third International Rushmore Conference on Enteric Diseases. Rapid City, SD.
Lindblom SJ, SJ Vilain, VS Brozel, RS Kaushik, S George, HH Stein, C Pedersen, D Francis, AJM Rosa. 2005. Characterization of microbial communities in weanling pigs using 16S rRNA gene sequences. Abstract. Third International Rushmore Conference on Enteric Diseases. Rapid City, SD.
Zhang W, M Zhao, and D Francis. 2005. Mapping the Binding Domains of K88ac Fimbrial Adhesin. Abstract. Conference of Research Workers in Animal Diseases, Annual Mtg. St Louis MO.

WA
1. Bae, W, KN Kaya, DD Hancock, DR Call, YH Park, and TE Besser. 2005. Prevalence and antimicrobial resistance of thermophilic Campylobacter sp. from cattle farms in the Northwestern United States. Applied and Environmental Microbiology 71:169-174.
2. Borucki, MK, J Reynolds, DR Call, T Ward, B Page, and J Kadushin. 2005. Suspension arrays for direct and high throughput subtyping of Listeria monocytogenes from genomic DNA. Journal of Clinical Microbiology 43:3255-3259.
3. Call, DR. 2005. Challenges and opportunities for pathogen detection using DNA microarrays. Critical Reviews in Microbiology 31:91-99.
4. Call, DR, MS Kang, J Daniels, and TE Besser. Accepted. Assessing genetic diversity in plasmids from Escherichia coli and Salmonella enterica using a mixed-plasmid microarray. Journal of Applied Microbiology.
5. Wright JG, Tengelsen LA, Smith KE, Bender JB, Frank RK, Grendon JH, Rice DH, Thiessen AM, Gilbertson CJ, Sivapalasingam S, Barrett TJ, Besser TE, Hancock DD,Angulo FJ. 2005. Multidrug-resistant Salmonella Typhimurium in four animal facilities. Emerg Infect Dis. 11(8):1235-41.
6. Besser TE, LeJeune JT, Rice DH, Berg J, Stilborn RP, Kaya KN, Bae W, Hancock DD. 2005. Increasing prevalence of Campylobacter jejuni in feedlot cattle through the feeding period. Appl. Environ Microbiol. In press.
7. Cobbold, R.N.; Rice, D.H.; Davis, M.A.; Besser, T.E.; Hancock, D.D. Long-term Persistence of Multi-drug resistant (MDR) Salmonella enterica Serovar Newport in Two Washington Dairy Herds. Journal of the American Veterinary Medical Association. In press.
8. Borucki, MK, CC Gay, J Reynolds, KL McElwain, SH Kim, DR Call, DP Knowles. Genetic diversity of Listeria monocytogenes strains from a highly contaminated dairy farm. Applied and Environmental Microbiology. In press.

12/27/2006

A. Journal Articles and other Outputs: jointly authored by multiple NC-1007 stations.

Zhang, W., E. M. Berberov, J. Freeling, D. He, R. A. Moxley, and D. H. Francis. 2006. Significance of heat-stable and heat-labile enterotoxins in porcine colibacillosis in an additive model for pathogenicity studies. Infect. Immun. 74:3107-3114.

Wang, Q., K. O. Chang, M.G. Han, S. Sreevatsan, and L. J. Saif. 2006. Development of a new microwell hybridization assay and an internal control RNA for the detection of porcine noroviruses and sapoviruses by reverse transcription-PCR. J. Virol. Meth. 132:135-45.

Wang, Q.H., M. Souza, J.A. Funk, W. Zhang, and L.J. Saif. 2006. Prevalence of noroviruses and sapoviruses in swine of various ages determined by reverse transcription-PCR and microwell hybridization assays. J. Clin. Microbiol. 44:1575-1578.

Costantini, V., F. Loisy, L. Joens, F.S. LeGuyader and L.J. Saif. 2006. Human and animal enteric caliciviruses in oysters from different coastal regions of the U.S. Applied and Environmental Microbiology 72:1800-9. NC Collaborator Joens, AZ



B. Journal Articles from NC-1007 stations:

Bae W, Hancock DD, Call DR, ParkYH, Berge ACB, Finger RM, Sischo WM, Besser TE. Dissemination of Clonal Strains of Campylobacter coli Resistant to Multiple Antimicrobial Drugs among Washington State and California Cattle. In press, Veterinary Microbiology, 2006.

Besser TE, Shaikh N, Holt NJ, Tarr PI, Konkel ME, Malik-Kale P, Whittam T, Bono J.. 2007. Greater Diversity of Shiga toxin-encoding Bacteriophage Insertion Sites among Escherichia coli O157:H7 Isolates from Cattle than from Humans. Appl. Environ. Microbiol. 73:671-9.

Bretschneider, G., E. M. Berberov, and R. A. Moxley. Reduced intestinal colonization of adult beef cattle by Escherichia coli O157:H7 tir deletion and nalidixic acid-resistant mutants lacking flagellar expression. Appl. Environ. Microbiol. (Submitted)

Call DR, Kang M-S, Daniels J, Besser TE. 2006. Assessing genetic diversity in plasmids from Escherichia coli and Salmonella enterica using a mixed-plasmid microarray. Journal of Applied Microbiology 100(1):15-28.

Carvajal, A., M.L. De Arriba, H. Rodriguez, A.B. Vidal, G.E. Duhamel, and P. Rubio. 2006. Prevalence of Brachyspira species in pigs with diarrhoea in Spain. Vet. Rec. 158:700-701.

Clark, N. M., E. M. Berberov, M. Wang, and R. A. Moxley. 2006. Anti-capsular antibodies activate killing of Escherichia coli O8:K87 by the alternate complement pathway in porcine serum. Vet. Immunol. Immunopathol. 114:185-191.

Cobbold RN, Hancock DD, Rice DH, Berg J, Stilborn R, Hovde CJ, Besser TE. 2007. Recto-anal Junction Colonization of Feedlot Cattle with E. coli O157:H7 and its Association with Super-shedders and Excretion Dynamics. In press. Appl Environ Microbiol.

Cobbold RL, Rice DH, Davis MA, Besser TE, Hancock DD: 2006. Long-term persistence of muli-drug resistant (MDR) Salmonella enterica Serovar Newport in two Washington dairy herds. Journal of the American Veterinary Medical Association 228:585-91.

Davis MA, Hancock DD, Besser TE, Daniels JB, Kaya K, Call DR. 2007. Antimicrobial resistance in Salmonella enterica serovar Dublin isolates from beef and dairy sources. Vet Microbiol. 31:221-30.

Davis MA, Rice DH, Sheng H, Hancock DD, Besser TE, Cobbold R, Hovde CJ: 2006. Comparison of cultures from rectoanal junction mucosal swabs and feces for detection of Escherichia coli O157 in dairy heifers. Applied and Environmental Microbiology 72:3766-70.

Deprez, P., K. Chiers, C. J. Gebhart. 2005. Lawsonia intracellularis infection in a 12-month-old colt in Belgium. Vet. Rec. 157:774-776.

Han, M.G., S. Cheetham, MS.P. Azevedo, C. Thomas and L. J. Saif. 2006. Immune responses to bovine norovirus-like particles with various adjuvants and analysis of protection in gnotobiotic calves. Vaccine 24:317-26.

Han, M.G., D-S. Cheon, X. Zhang and L.J. Saif. 2006. Cross-protection in gnotobiotic calves between a human enteric coronavirus and a virulent bovine enteric coronavirus. J. Virol.80:12350-6.

Kang M-S, Besser TE, Call DR. 2006. Diversity of Genetic Contexts of the blaCMY-2 ²-Lactamase Gene in Escherichia coli and Salmonella enterica plasmids that confer resistance to expanded-spectrum cephalosporins. Antimicrobial Agents and Chemotherapy 50):1590-3.

Khachatryan AR, Hancock DD, Besser TE, Call DR: 2006. Antimicrobial drug resistance genes do not convey a secondary fitness advantage to calf-adapted Escherichia coli. Applied Environmental Microbiology 72:443-8.

LeJeune JT, Hancock DD, Besser TE: 2006. Sensitivity of Esherichia coli O157 detection in bovine feces using broth enrichment followed by immunomagnetic separation and direct plating methodologies. Journal of Clinical Microbiology 44:872-5.

Lim JY, Li J, Sheng H, Besser TE, Potter K, Hovde CJ. 2006. Colonization of Escherichia coli O157:H7 at the recto-anal junction of long-duration culture-positive cattle. Appl Environ Microbiol. In press, 2006.

McOrist, S., C. J. Gebhart and B. Bosworth. 2006. Evaluation of porcine ileum models of enterocytes infection by Lawsonia intracellularis. Can. J. Vet. Res. 70:155-159.

Petersen, R. E., T. J. Klopfenstein, G. E. Erickson, J. Folmer, S. Hinkley, R. A. Moxley, and D. R. Smith. 2007. Effect of Lactobacillus acidophilus strain NP51 on Escherichia coli O157:H7 fecal shedding and finishing performance in beef feedlot cattle. J. Food Prot. 70: (in press).

Peterson, R. E., T. J. Klopfenstein, R. A. Moxley, G. E. Erickson, S. Hinkley, G. Bretschneider, E. M. Berberov, D. Rogan, and D. R. Smith. Effect of a type III secreted protein vaccine on Escherichia coli O157:H7 fecal shedding and rectal colonization of feedlot cattle. J. Food Prot. (Submitted)

Peterson, R. E., T. J. Klopfenstein, R. A. Moxley, G. E. Erickson, S. Hinkley, D. Rogan, and D. R. Smith. Evaluation of dose response and herd immunity from a vaccine against Escherichia coli O157:H7 for feedlot cattle. J. Food Prot. (Submitted)

Wang, Q-H., V. Costantini, and L.J. Saif. 2007. Porcine and human enteric caliciviruses: Comparative diagnosis, epidemiology and genetic and antigenic relatedness. Vaccine (in press).

Wattanaphansak, S., C.J. Gebhart, M. Olin and J. Deen. 2005. Measurement of the viability of Lawsonia intracellularis. Can. J. Vet. Res. 69:265-271.


Extension Reports:

Peterson, R.E., D.R. Smith, R.A. Moxley, T.J. Klopfenstein, S. Hinkley, and G.E. Erickson. 2006. Vaccination for Escherichia coli O157:H7 in market ready feedlot cattle. 2006 Nebraska Beef Report, Agricultural Research Division, University of Nebraska Cooperative Extension, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, MP88-A. pp. 68-69.

Peterson, R.E., D.R. Smith, R.A. Moxley, T.J. Klopfenstein, S. Hinkley, and G.E. Erickson. 2006. Large-scale clinical trial to evaluate an experimental Escherichia coli O157:H7 vaccine. 2006 Nebraska Beef Report Agricultural Research Division, University of Nebraska Cooperative Extension, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, MP88-A. pp. 70-71.

Book Chapters:

Hampson, D.J. and G.E. Duhamel. 2006. Porcine colonic spirochetosis/Intestinal spirochetosis. In: Diseases of Swine. 9th ed. Straw, B.E., J.J. Zimmerman, S. DAllaire, and D.J. Taylor (eds). Blackwell Publishing Ltd, Ames, Iowa, pp. 755-767.

Duhamel, G.E. Neonatal calf enteric disease vaccines. In: Large Animal Internal Medicine. 4th ed., Smith, B.P. (ed). Mosby, Inc., St. Louis, Missouri, in press.

McOrist, S. and C.J. Gebhart. 2005. Genus Lawsonia. In G.E. Garrity, et al, (ed.), Bergeys Manual of Systematic Bacteriology, 2nd ed. Williams and Wilkins, Baltimore, MD.

McOrist, S. and C.J. Gebhart. 2005. Porcine proliferative enteropathies. In B.E. Straw, et al. (ed), Diseases of Swine, 9th ed. Iowa State University Press, Ames, IA.

Other Presentations and Published Abstracts:

The effect of regional vaccination within the feedyard on Escherichia coli O157:H7 colonization, fecal shedding, and hide contamination. Smith, D.R., R.A. Moxley, T.J. Klopfenstein, G.E. Erickson. National Cattlemens Beef Association, Beef Industry Safety Summit, Jacksonville, Florida, April 18-20, 2006, Oral. Published - Beef Industry Safety Summit Executive Summary, Beef Industry Food Safety Council, pp. 10-11.

Effect of vaccinating against type III secreted proteins of E. coli O157:H7 on its pre- and post-harvest occurrence on cattle hides. Peterson, R.E., D.R. Smith, T.J. Klopfenstein, G.E. Erickson. Joint Meeting of the American Dairy Science Association (ADSA) and American Society of Animal Science (ASAS) Minneapolis, Minnesota, July 9-13, 2006, Oral. Published - abstract no. 269, J. Anim. Sci. Vol. 84, Suppl. 1/J. Dairy Sci. Vol. 89, Suppl. 1, page 250.

Effect of vaccination within the feedyard on Escherichia coli O157:H7 colonization, fecal shedding, and hide contamination. Moxley, R.A., D.R. Smith, T.J. Klopfenstein, G.E. Erickson. Cattle Industry Summer Conference, Beef Safety Committee, Reno, Nevada, July 12, 2006, Oral - no publication.

Does vaccinating cattle against type III secreted proteins of Escherichia coli O157:H7 prevent colonization? Smith, D.R., R.E. Peterson, R.A. Moxley, T.J. Klopfenstein, G.E. Erickson, S. Hinkley. International Symposium on Veterinary Epidemiology and Economics (ISVEE XI), Cairns, Australia, Aug. 6-11, 2006, Poster - published abstract.

Vaccination to control Escherichia coli O157:H7 in feedlot cattle. Moxley, R.A. Invited Seminar Speaker, Department of Veterinary Science and Department of Biology/Microbiology, Molecular and Cellular Biology Seminar Series, South Dakota State University, Brookings, South Dakota. Oct. 13, 2006.

Reduced intestinal colonization of adult beef cattle by Escherichia coli O157:H7 tir deletion and nalidixic acid -resistant mutants lacking flagellar expression. Bretschneider, G., E.M. Berberov, R.A. Moxley. 6th International Symposium on Shiga Toxin (Verocytotoxin) Producing E. coli Infections (VTEC 2006), Melbourne, Australia, Oct. 29-Nov. 1, 2006, poster no. P09.1.04 - published abstract.

Effect of Lactobacillus acidophilus strain NP51 on Escherichia coli O157:H7 fecal shedding and finishing performance in beef feedlot cattle. Moxley, R.A., D.R. Smith, T.J. Klopfenstein, G.E. Erickson, J.D. Folmer, R.E. Peterson, S. Hinkley. 6th International Symposium on Shiga Toxin (Verocytotoxin) Producing E. coli Infections (VTEC 2006), Melbourne, Australia, Oct. 29-Nov. 1, 2006, poster no. P11.2.05 - published abstract.

Effect of a type III secreted protein vaccine on Escherichia coli O157:H7 fecal shedding and rectal colonization of feedlot cattle. Moxley, R.A., D.R. Smith, T.J. Klopfenstein, G.E. Erickson, R.E. Peterson, S. Hinkley, G. Bretschneider, E.M. Berberov, D. Rogan. 6th International Symposium on Shiga Toxin (Verocytotoxin) Producing E. coli Infections (VTEC 2006), Melbourne, Australia, Oct. 29-Nov. 1, 2006, poster no. P11.2.06 - published abstract.

A large-scale clinical trial evaluating an Escherichia coli O157:H7 type III secreted protein vaccine for cattle in commercial feedlot systems. Smith, D.R., R.A. Moxley, R.E. Peterson, T.J. Klopfenstein, G.E. Erickson, S. Hinkley, G. Bretschneider, E.M. Berberov. 6th International Symposium on Shiga Toxin (Verocytotoxin) Producing E. coli Infections (VTEC 2006), Melbourne, Australia, Oct. 29-Nov. 1, 2006, poster no. P12.1.04 - published abstract.

Effect of dosage number of an Escherichia coli O157:H7 type III secreted protein vaccine on fecal shedding and herd immunity in feedlot cattle. Smith, D.R., R.A. Moxley, R.E. Peterson, T.J. Klopfenstein, G.E. Erickson, S. Hinkley, D. Rogan. 6th International Symposium on Shiga Toxin (Verocytotoxin) Producing E. coli Infections (VTEC 2006), Melbourne, Australia, Oct. 29-Nov. 1, 2006, poster no. P12.1.05 - published abstract.

Serological IgG responses against Escherichia coli O157:H7 type III secreted proteins, intimin and O157 lipopolysaccharide in adult beef cattle following experimental infection. Bretschneider, G., E.M. Berberov, R.A. Moxley. Conference of Research Workers in Animal Diseases, Chicago, Illinois, Dec. 3-5, 2006, poster, published - abstract no. P65.

Effect of regional vaccination within the feedyard on Escherichia coli O157:H7 rectal colonization, fecal shedding, and hide contamination. Smith, D.R., R.A. Moxley, T.J. Klopfenstein, G.E. Erickson. Conference of Research Workers in Animal Diseases, Chicago, Illinois, Dec. 3-5, 2006, oral, published - abstract no. 110.

Wattanaphansak, S.W., T. Asawakarn, C.J.Gebhart and J. Deen. 2006. Development and validation of an ELISA for the diagnosis of PPE. Proc. 19th IPVS, Denmark.

Wattanaphansak, S.W., T. Asawakarn, C.J.Gebhart and J. Deen. 2006. Use of the recombinant protein FliC for development of an elisa for the diagnosis of PPE. Proc. 19th IPVS, Denmark.

Guedes, R.M.C. and C. Gebhart. 2006. Enterocyte proliferation and apoptotic changes in ileum samples from pigs experimentally infected with Lawsonia intracellularis. Proc. 19th IPVS, Denmark.

Gebhart, C. 2006. Lawsonia intracellularis infections (Invited Key Note Lecture). Proc. 19th IPVS, Denmark.

Kinsley, K, N. Winkelman, and C. Gebhart. 2006. Elimination of Lawsonia intracellularis from pigs fed carbadox. Proc. Am. Assoc. Swine Vet., Kansas City, MO.