02/17/2025

Publications

1. Denicol AC. What we can learn from the bovine embryo and mouse models to enable in vitrogametogenesis in cattle. Reproduction, Fertility and Development 37, RD24142. 2025. doi:10.1071/RD24142


2. Altman MA, Mathews AT, Rabaglino MB, Hovey RC, Denicol AC. Canonical prolactin signaling and global mRNA expression in the skin of Holstein heifers carrying the SLICK1 allele of the prolactin receptor gene. J of Dairy Sci 2025 (ACCEPTED).


3. Candelaria JI, Botigelli RC, Guiltinan C, Shikanov A, Denicol AC. Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles. bioRxiv [Preprint]. 2024 Aug 7:2024.07.18.604061. doi: 10.1101/2024.07.18.604061.


4. Kennedy VC, Lynch CS, Tanner AR, Winger QA, Gad A, Paul J. Rozance PJ, Anthony RV. Fetal Hypoglycemia Induced by Placental SLC2A3 RNA Interference Alters Fetal Pancreas Development and Transcriptome at Mid-Gestation. Int. J. Mol. Sci. 2024, 25(9), 4780; https://doi.org/10.3390/ijms25094780.


5. Tanner AR, Kennedy VC, Lynch CS, Winger QA, Anthony RV, Rozance PJ. Increasing maternal glucose concentrations is insufficient to restore placental glucose transfer in chorionic somatomammotropin RNA interference pregnancies. Am J Physiol Endocrinol Metab. 2024 Feb 14.


6. Xiao-Kim E, Graham JK, Schountz T, Barfield JP. 2024. Osmotic tolerance and cryopreservation of Jamaican fruit bat (Artibeus Jamaicensis) sperm. Therio Wild, vol 6, 100118.


7. Nico G Menjivar, Jaiden Oropallo, Samuel Gebremedhn, Luca A Souza, Ahmed Gad, Christian M Puttlitz, Dawit Tesfaye. 2024. MicroRNA Nano-Shuttles: Engineering Extracellular Vesicles as a Cutting-Edge Biotechnology Platform for Clinical Use in Therapeutics. Biol Proced Online 26, 14.


8. Joyce K, Gad A, Menjivar NG, Gebremedhn S, Heredia D, Dubeux G, Lopez-Duarte MC, Bittar J, Gonella-Diaza A, Tesfaye D. 2024. Seasonal environmental fluctuations alter the transcriptome dynamics of oocytes and granulosa cells in beef cows. J Ovarian Res;17(1):201.


9. Feugang JM, Gad A, Menjivar NG, Ishak GM, Gebremedhn S, Gastal MO, Dlamini NH, Prochazka R, Gastal EL, Tesfaye D. 2024. Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid. J Anim Sci Biotechnol;15(1):137.


10. Dessie Salilew-Wondim, Michael Hoelker, Eva Held-Hoelker, Franca Rings, Ernst Tholen, Christine Große-Brinkhaus, Karl Shellander, Carina Blaschka, Urban Besenfelder, Vita Havlicek, Dawit Tesfaye. 2024. Sexual dimorphic miRNA-mediated response of bovine elongated embryos to the maternal microenvironment. PLoS ONE 19(2).


11. Ahmed Gad, Nico G Menjivar, Rachel Felton, Barbara Durrant, Dawit Tesfaye, Elena Ruggeri. 2024. Mapping the follicle-specific regulation of extracellular vesicle-mediated microRNA transport in the southern white rhinoceros (Ceratotherium simum simum). Biology of Reproduction. ioae081.


12. Eva Held-Hoelker, Jessica Kurzella, Dessie Salilew-Wondim, Franca Rings, Dawit Tesfaye, Ernst Tholen, Christine Grosse-Brinkhaus, Michael Hoelker. 2024. L-Carnitine sustainably affects bioenergetic profile of bovine blastocysts and transcriptome profile of elongation-stage embryos. Reproduction Aug 2;168(3):e240004.


13. Jessica Kurzella, Dennis Miskel, Franca Rings, Ernst Tholen, Dawit Tesfaye, Karl Schellander, Dessie Salilew-Wondim, Eva Held-Hoelker, Christine Große-Brinkhaus, Michael Hoelker. 2024. Mitochondrial bioenergetic profiles of warmed bovine blastocysts are typically altered after cryopreservation by slow freezing and vitrification. Theriogenology 214, 21-32.


14. Jessica Kurzella, Dennis Miskel, Franca Rings, Ernst Tholen, Dawit Tesfaye, Karl Schellander, Dessie Salilew-Wondim, Eva Held-Hoelker, Christine Große-Brinkhaus, Michael Hoelker: 2024. Mitochondrial bioenergetic profiles of warmed bovine blastocysts are typically altered after cryopreservation by slow freezing and vitrification. Theriogenology 214, 21-32.


15. Zhu J, Lai Y, Cheng M, Dash RC, Guo S, Guo J, Su Y, Wolek A, Issacs B, Liu Z, Li Q, Mishra N, *Garmendia A, *Hadden MK, *Tian XC, *He X, *Tang Y. 2024. Discovery of Small Molecules Against Porcine Reproductive and Respiratory Syndrome Virus Replication by Targeting NendoU Activity. Journal of Virology ; https://doi.org/10.1128/jvi.02034-24


16. Ming H, Scatolin G, Ojeda A, Jiang Z#. Establishment of bovine extraembryonic endoderm cells. bioRxiv [Preprint]. 2024 Dec 18. https://biorxiv.org/cgi/content/short/2024.12.17.628911v1


17. Zhu L, Ming H, Scatolin G, Xiao A, Jiang Z#. METTL7A improves bovine IVF embryo competence by attenuating oxidative stress. bioRxiv [Preprint]. 2024 Dec 18. https://biorxiv.org/cgi/content/short/2024.12.17.628915v1


18. Ming H, Iyyappan R, Kakavand K, Dvoran M, Susor A, Jiang Z#. Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development. bioRxiv [Preprint]. 2024 Oct 28:2024.10.28.620693. doi: 10.1101/2024.10.28.620693. PMID: 39553972.


19. Logsdon DM, Ming H, Ezashi T, West RC, Schoolcraft WB, Roberts RM, Jiang Z#, Yuan Y#. Transcriptome comparisons of trophoblasts from regenerative cell models with peri-implantation human embryos†. Biol Reprod. 2024 Nov 11;111(5):1000-1016. doi: 10.1093/biolre/ioae120. PMID: 39109839.


20. Ming H, Zhang M, Rajput S, Logsdon D, Zhu L, Schoolcraft WB, Krisher RL, Jiang Z#, Yuan Y#. In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†. Biol Reprod. 2024 Jul 12;111(1):11-27. doi: 10.1093/biolre/ioae031. PMID: 38408205.


21. Znachorova T, Dudko N, Ming H, Jiang Z, Fulka H. The timing of pronuclear transfer critically affects the developmental competence and quality of embryos. Mol Hum Reprod. 2024 Jun 26;30(7):gaae024. doi: 10.1093/molehr/gaae024. PMID: 38991843.


22. Xu S, Wang N, Zuccaro MV, Gerhardt J, Iyyappan R, Scatolin GN, Jiang Z, Baslan T, Koren A, Egli D. DNA replication in early mammalian embryos is patterned, predisposing lamina-associated regions to fragility. Nat Communications. 2024 Jun 19;15(1):5247. doi: 10.1038/s41467-024-49565-7. PMID: 38898078.


23. Lamacova L, Jansova D, Jiang Z, Dvoran M, Aleshkina D, Iyyappan R, Jindrova A, Fan HY, Jiao Y, Susor A. CPEB3 Maintains Developmental Competence of the Oocyte. Cells. 2024 May 16;13(10):850. doi: 10.3390/cells13100850. PMID: 38786074.


24. Scatolin GN, Ming H, Wang Y, Iyyappan R, Gutierrez-Castillo E, Zhu L, Sagheer M, Song C, Bondioli K, Jiang Z#. Single-cell transcriptional landscapes of bovine peri-implantation development. iScience. 2024 Mar 27;27(4):109605. doi: 10.1016/j.isci.2024.109605. PMID: 38633001.


25. Fendereski M, Ming H, Jiang Z, Guo Y Mouse trophoblast cells have attenuated responses to TNF and IFN and can avoid synergic cytotoxicity of the two cytokines.. Journal of Immunology. 2024 Jan 15. PMID: 38054905.


26. Guertin JE, Losano JDA, Salazar S, Callaham J, Daigneault, BW. Prolonged maintenance of cooled stallion semen by optimization of storage conditions. Journal of Equine Veterinary Sciences. 2024 Nov 19;144:105243. doi: 10.1016/j.jevs.2024.105243. Online ahead of print.


27. McGraw MJ, Bishman JA, Daigneault BW. Blastomere complementation efficiency and pluripotency maintenance following multiple passaging of in vitro derived bovine embryos. Reproduction, Fertility and Development. 2024 Jun:36:RD24018. doi: 10.1071/RD24018.


28. Camila J. Cuellar,Thiago F. Amaral, Paula Rodriguez-Villamil, F. Ongaratto, D. Onan Martinez,Remi Labreque,Jo D.A. Losano,Eliab Estrada-Cortes, Jonathan R. Bostrom, Kyra Martins, D. Owen Rae, Jeremy Block, Quinn A. Hoorn, Bradford W. Daigneault, Jonathan Merriam, Michael Lohuis, Serdal Dikmen, Joao Bittar,Tatiane S. Maia, Daniel F. Carlson, Sabreena Larson, Tad S. Sonstegard, and Peter J. Hansen. Consequences of gene editing of PRLR on thermotolerance, growth, and reproduction in cattle. FASEB Bioadv. 2024 2024 Jun 18;6(8):223-234. doi: 10.1096/fba.2024-00029. eCollection 2024 Aug


29. Cesar A. Rosales-Nieto, Bradford W. Daigneault, Jennifer N. Roberts, Rodrigo Sanchez-Lopez, Barbara Makela, Yong Pu, Richard Ehrhardt, Kristi L Jones, Joao H. Jabur Bittar, Almudena Veiga-Lopez. Birth weight, growth indices, and seminal parameters in male offspring are resilient features to maternal pre-conceptional dietary manipulation in sheep. Domest Anim Endocrinol. 2024 Apr 7:88:106849. doi: 10.1016/j.domaniend.2024.106849.


30. McGraw MJ, Rajput SK, Daigneault BW. Peroxisome proliferator-activated receptor gamma influences developmental competence and early placental formation in bovine embryos. Reproduction. 2024 Jan 31;167(2):e230334. doi: 10.1530/REP-23-0334. Print 2024 Feb 1.


31. Stella, S., Guadagnin, A.R.,Velasco-Acosta, D., Ferreira, C., Rubessa, M., Wheeler, M.B., Luchini, D., Cardoso, F. (2024) Rumen-Protected Methionine Supplementation Alters Lipid Profile of Preimplantation Embryo and Endometrial Tissue of Holstein Cows. Front. Vet. Sci., 17 January 2024. Sec. Animal Nutrition and Metabolism, Volume 10 - 2023 | https://doi.org/10.3389/fvets.2023.1301986


32. Wheeler, M.B., Rabel, R.A. Chanaka, Rubessa, M., and Gabriel Popescu (2024) Invited Paper - Label-Free, High-Throughput Holographic Imaging to Evaluate Mammalian Gametes and Embryos. Biology of Reproduction, 2024, ioae057, https://doi.org/10.1093/biolre/ioae057


33. Monzani, P.S., Sangalli, J.R., Sampaio, R.V., Guemra, S., Zanin, R., Adona, P.R., Berlingieri, M.A., Cunha-Filho, L.F.C, Mora- Ocampo, I.Y., Pirovani, C.P, Meirelles, F.V., Wheeler, M.B., Ohashi, O.M. (2024). Human proinsulin production in the milk of transgenic cattle. Biotechnology Journal, 19, e2300307. https://doi.org/10.1002/biot.202300307


34. Geng Wang, Lianhuang Li, Xiaoxia Liao, Shu Wang, Jennifer Mitchell, R.A. Chanaka Rabel, Shirui Luo, Jindou Shi, Janet E. Sorrells, Rishyashring R. Iyer,


35. Edita Aksamitiene, Carlos A. Renteria, Eric J. Chaney, Derek J. Milner, Matthew B. Wheeler, Martha U. Gillette, Alexander Schwing,Jianxin Chen, Haohua Tu (2024). Supercontinuum intrinsic fluorescence imaging heralds ‘free view’ of living systems. bioRxiv https://doi.org/10.1101/2024.01.26.577383.


36. Bangert, E. A., C. Allen, A. Chota, G. Msuta, E. Mbisha and M. B. Wheeler (2024). Assessment of Challenges and Recommendations for Success When Implementing Assisted Reproductive Technology Programs in Developing Countries in the Tropics (Tanzania).


37. CABI Animal Science Cases 2024. DOI: 10.1079/animalsciencecases.2024.0012


38. Rabel, R.A.C., Bangert, E.A., Wilson, K., Wheeler, M.B. (2024). The Use of Assisted Reproductive Technologies to Improve Genetic Selection in Cattle. In: Gardón, J.C., Satué Ambrojo, K. (eds) Assisted Reproductive Technologies in Animals Volume 1. Springer, Cham. https://doi.org/10.1007/978-3-031-73079-5_8


39. Sun, N.N. Ali, D. Londoño-Vásquez, C.A. Simintiras, H. Qiao, M.S. Ortega, Y. Agca, M.M. Takahashi, R.M. Rivera, A.M. Kelleher, P. Sutovsky, A.L. Patterson, A.Z. Balboula*. (2024) Increased DNA damage in full-grown oocytes is correlated with diminished autophagy activation. Nature Communications, 1, 15(1): 9463. * Corresponding author.


40. Kiesler ZG, Hunter MI, Balboula AZ, Patterson AL. (2024). Periostin's role in uterine leiomyoma development: a mini review on the potential periostin poses as a pharmacological intervention for uterine leiomyoma. Archives of Gynecology and Obstetrics, 309(5): 1825-1831.


41. Elmetwally MA, Helmy A, Zaabel S, Montaser A, Balboula A, Eladl M, Hamed B, Lashen S, Rezk S, Yaseen A, Sharawy H, Hussien M, Halawa A. (2024). Fructose improves Titanium Dioxide Nanoparticles induced alterations in Developmental Competence of mouse oocytes. BMC Veterinary Research, 3, 20(1): 135.


42. Redel BK, Yoon J, Reese E, An H, Uh K, Chen PR, Prather RS, Lee K. Novel off-Targeting Events Identified after Genome Wide Analysis of CRISPR-Cas Edited Pigs. CRISPR J. 2024 May 21. doi: 10.1089/crispr.2024.0012. Online ahead of print.


43. Sun, P. Sutovsky, A.L. Patterson, A.Z. Balboula AZ*. (2023). Mechanisms of DNA damage response in mammalian oocytes, Advances in Anatomy, Embryology and Cell Biology series. 238:47-68. * Corresponding author.


44. Arner, E.S.J., Schmidt, E.E., 2024. Unresolved questions regarding cellular cysteine sources and their possible relationships to ferroptosis. Advances in cancer research 162, 1-44.


45. Borbényi-Galambos, K., Erdélyi, K., Ditrói, T., Jurányi, E.P., Szántó, N., Szatmári, R., Czikora, Á., Schmidt, E.E., Garai, D., Cserepes, M., Liszkay, G., Tóth, E., Tóvári, J., Nagy, P., 2025. Realigned Transsulfuration Drives BRAF V600E-Targeted Therapy Resistance in Melanoma. Cell Metabolism In revision.


46. Martí-Andrés, P., Finamor, I., Torres-Cuevas, I., Pérez, S., Rius-Pérez, S., Colino-Lage, H., Guerrero-Gómez, D., Morato, E., Marina, A., Michalska, P., León, R., Cheng, Q., Jurányi, E.P., Borbényi-Galambos, K., Millán, I., Nagy, P., Miranda-Vizuete, A., Schmidt, E.E., Martínez-Ruiz, A., Arnér, E.S., Sastre, J., 2024. TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. The EMBO Journal 43, 2789-2812.


47. Schmidt, E.E., Miller, C.G., Juranyi, E.P., Austad, S.N., Ditroi, T., Seaford, Z.M., Yoon, S.J., Noyd, R.C., Kang, Y.P., Prigge, J.R., Ciskos, V., Kovari, D., DeNicola, G.M., Nagy, P., 2025. Cystine C-S bond cleavage fuels cysteine production under disulfide reductase deficiency. Natue Chemical Biology In revision.


48. R.Biga*, J. E. Duan*, T E. Young*, J. R. Marks*, A. Bronikowski3*,L. P. Decena3*, E. C. Randolph1*, A. G. Pavuluri*, G. Li*, Y. Fang*, G. S. Wilkinson*, G. Singh#, N. T. Nigrin6#, E. N. Larschan6#, A. J. Lonski#, Nicole C. Riddle1^, and IISAGE Consortium (2024). Hallmarks of Aging: A User's Guide for Comparative Biologists, in press. Ageing Research Reviews


49. Su,Y., Zhao, R., Fang, Y., Li, G., Jin, L., Liu, J., Zhu, J., Mishra, N., Kaback, D., Yee, S., Lei, C., Duan, J.*, Tian, X.*, Tang, T.*  (2024) Under revision. Bovine Formative Embryonic Stem Cells Capable of Three-Germ Layer and Extraembryonic Differentiation.


50. Yu X., Harman R., Danev N., Li G., Fang Y., Van de Walle G., Duan J.E*. Under review. Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells. 2024 bioRxiv, https://www.biorxiv.org/content/10.1101/2024.05.15.594241v1


51. Aguilera J., Duan J., Lee S., Ray M., Larschan E. The CLAMP GA-binding transcription factor regulates heat stress-induced transcriptional repression by associating with 3D loop anchors. bioRxiv 2023.10.08.561401; doi: https://doi.org/10.1101/2023.10.08.561401


52. Li G, Danev, N, Duan, J*, Van de Walle G. (2024), Comparative transcriptomic analysis of bovine mesenchymal stromal cells reveals tissue-source and species-specific differences. 2024 Jan 12;27(2):108886. doi: 10.1016/j.isci.2024.108886. PMID: 38318381; PMCID: PMC10838956.


53. F.F. Bönmann, L.G.D. Mendonça, I. Sellmer Ramos, R. Fritz, C. Gamarra, D. Duhatschek, R.S.S. de Oliveira, A.L.A. Scanavez, T.S. Belem, M.C. Lucy, J.G.N. Moraes. Sensor-based and Visual Behavioral Profiling of Dry Holstein Cows Presenting Distinct Median Core Body Temperature. doi.org/10.3390/ani14192832. Animals 14, no. 19 (2024): 2832.


54. G.N. Moraes, S. E. Poock, A.C. Ericsson, T. Gull, Caldeira, M.O., and M. C. Lucy. 2024. The microbiome of the pregnant uterus in Holstein dairy heifers and cows assessed by bacterial culture and 16S ribosomal RNA gene sequencing. Front Microbiol. May 15:15:1385497. doi: 10.3389/fmicb.2024.1385497. eCollection 2024.


55. G.N. Moraes, T. Gull, A.C. Ericsson, S. E. Poock, Caldeira, M.O., and M. C. Lucy. 2024. Establishment of the uterine microbiome following artificial insemination in virgin heifers. Front Microbiol. Jun 5:15:1385505. doi: 10.3389/fmicb.2024.1385505. eCollection 2024.


56. Sellmer Ramos, M. O. Caldeira, S. E. Poock, J.G.N. Moraes, M. C. Lucy, and A.L. Patterson. Adenomyosis and fibrosis define the morphological memory of the postpartum uterus of dairy cows previously exposed to metritis. JDS Communications. 2024 Oct 30. doi.org/10.3168/jdsc.2024-0633


57. G. da Silva, L.G. da Silva, L.C.L. Ferreira, J. Mascarello, J.C.B. Silva, J.G.N. Moraes, M.C. Lucy, E. Nogueira. Factors influencing pregnancy per artificial insemination (AI) and embryonic mortality in Nelore females subjected to timed-AI in Brazil. Anim Reprod Sci. 2024 Jun:265:107475. doi: 10.1016/j.anireprosci.2024.107475.


58. C. C. Silva, M. O. Caldeira, J.G.N. Moraes, I. Sellmer Ramos, T. Gull, A. C. Ericsson, S. E. Poock, T. E. Spencer, and M. C. Lucy. 2024. Metritis and the uterine disease microbiome are associated with long-term changes in the endometrium of dairy cows. Biol Reprod. 2024 May 5:ioae67. doi: 10.1093/biolre/ioae067.


59. Ing, N.H., Curley Jr., K.O., Welsh Jr., T.O., Johnson, L., Luense, L.J., Clement, T.M. “Anatomy and Physiology of the Male Reproductive System and Potential Targets of Toxicants.” In Comprehensive Toxicology, 3^rd Edition, Elsevier Science, 2024, In press


60. Dodd, A.R., Luense, L.J. Contribution of the paternal histone epigenome to the preimplantation embryo. 2024 Frontiers in Cell and Developmental Biology, 12, doi:0.3389/fcell.2024.1476312


61. Blocher, R., Liu, Y., Patrick, T., Polejaeva, I.A.* (2024) Cytokine-supplemented maturation medium enhances cytoplasmic and nuclear maturation in bovine oocytes. Animals, 14:1837.


62. Liu, Y*., Stott, R., Regouski, M., Fan, Z., Viotti Perisse, I., Patrick, T., Keim, J., Meng, Q., Polejaeva, I.A.* (2024) A retrospective analysis of sheep generated by somatic cell nuclear transfer. Theriogenology 227:102-111.


63. Leir, S-H., Tkachenko, S., Paranjapye, A., Meckler, F., Van Wettere, A.J., Kerschner, J.L., Kuznetsov,E., Schacht, M., Giiurkar, P., Regouski M., Viotti Perisse, I., Marriott C.M., Liu, Y., Bunderson, I., White, K.L., Polejaeva, I.A., Ann Harris* (2024) Stellate cells are in utero markers of pancreatic disease in cystic fibrosis. Molecular Medicine  30:115.


64. Adams, L., Liu, Y., Polejaeva, I.A.* (2024) Current status of interspecies somatic cell nuclear transfer and meta‚Äêanalysis of the effects of phylogenetic distance on embryonic and fetal development. Mammal Review. 54:387-403.

Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

1. Acevedo, Carolina. Development of a bison-specific embryo culture system through targeted supplementation of media with stage-specific growth factors, Spring 2024, in fulfillment of MS degree. Advisee of J. Barfield.


2. Gene-environment interactions contributing to early embryo development in cattle. Maura M. McGraw, PhD Thesis, University of Florida.


3. Optimization of sperm conditions for equine reproductive technologies. Jillian, E Guertin, MS Thesis, University of Florida.


4. Comparison of isolation procedures for cryopreserved stallion sperm compatible with equine in vitro fertilization. Lana Katz, University of Florida Undergraduate Scholar Thesis.


5. Brewer, A. “A Novel Goat Model to Characterize Male Fertility Following Paternal cannabidiol Exposure”. Louisiana State University Masters Thesis. 2024.


6. Gatenby, L. “Improving Bovine Intracytoplasmic Sperm Injection.” Louisiana State University Ph. D. dissertation. 2024.


7. Girka, E. “Metabolic Activity During Bovine Maturation in Vivo and in Vitro”. Louisiana State University Ph. D. Dissertation. 

Abstracts

1. Midkiff, Kristen A., Kegley, Elizabeth B., Cheek, Robin A., Galloway, Doug L., Reynolds, Jana L., Harness, Alexis, Looney, Charles R., Littlejohn, Brittni P., Powell, Jeremy G. (2024).  Effect of diets containing soybean co-products formulated for developing heifers on reproductive efficiency.  Journal of Animal Science.  102 (Supp. 3):356-357.  https://doi.org/10.1093/jas/skae234.407.  Graduate student (Ph.D.) presented in Oral Abstracts – Ruminant Nutrition.


2. Efird, Emily. E., Powell, Jeremy G., Looney, Charles R., Perkins, Tommy L. (2024).  Evaluation of factors influencing conception rate within a fixed-time artificial insemination protocol using sex-sorted semen.  Journal of Animal Science.  Vol 102 (Supp. 3):474-475.  https://doi.org/10.1093/jas/skae234.539.  Graduated student presented poster abstract in ASAS Graduate Student Poster Competition – MS.


3. Snider, Miriam A., Culp, Timothy C., Hopkins, Carson R., Moss, Nathaniel T., Edwards, J. Lannett, Looney, Charles R., Powell, Jeremy G., Kegley, Elizabeth B., Coffey, Ken P.; Gadberry, M. Shane, Lemley, Caleb. O., Littlejohn, Brittni P. (2024).  Influence of melatonin supplementation in pregnant multiparous crossbred beef cows grazing endophyte-infected tall fescue on dam weight, milk yield, and pre-weaning growth of steer calves.  Journal of Animal Science.  Vol 102(Supp. 3):206-207.  https://doi.org/10.1093/jas/skae234.242.  Graduate student presented oral abstract – Growth and Development.


4. Snider, Miriam A., Culp, Timothy C., Hopkins, Carson R., Moss, Nathaniel T., Edwards, J. Lannett, Looney, Charles R., Powell, Jeremy G., Kegley, Elizabeth B., Coffey, Ken P. Gadberry, M. Shane, Lemley, Caleb O., Littlejohn, Brittni P. (2024).  Influence of melatonin supplementation on uterine and coccygeal artery hemodynamics of pregnant multiparous crossbred beef cows grazing endophyte-infected tall fescue.  Journal of Animal Science.  Vol102(Supp.3):284-285.  https://doi.org/10.1093/jas/skae234.326.  Graduate student presented oral abstract – Physiology and Endocrinology.


5. Efird, Emily. E., Gilbreath, E., Powell, Jeremy G., Moss, Nathaniel T., Gadberry, M. Shane, Looney, Charles R. (2024).  Effect of Semen Type and Fixed-Time Artificial Insemination Protocol on Pregnancy Rates for Beef Cows and Heifers.  Applied Reproductive Strategies in Beef Cattle Conference.  Graduate student presented oral abstract.


6. Showe, Clayton E., Weber, Wyatt E., Kutz, B. R., Shoulders, Benjamin P. Shoulders, Reynolds, Jana L., Powell, Jeremy P., Looney, Charles R. (2024).  Comparison of GnRH Administration Day in the 7 & 7 Synch Versus 8 & 6 Synch Protocol for Fixed-Time Artificial Insemination in Beef Heifers.  Journal of Animal Science.  101 (Supp. 1):57-58. 


7. Denicol, Anna, 2025 Conference Abstracts. Reproduction, Fertility and Development 37, RDv37n1abs. doi:10.1071/RDv37n1abs


8. Singleton SA, Kennedy VC, Donovan A, Anthony RV, Winger QA. Placenta-Specific RNA Interference of Transthyretin in Sheep. Society for the Study of Reproduction 56th Annual Meeting, Dublin, Ireland.


9. Kennedy VC, Tanner AR, Lynch CS, Winger QA, Rozance PJ, Anthony RV. Impact of Placental SLC2A3 RNA Interference throughout Gestation. Society for Reproductive Investigation 71st Annual Scientific Meeting, Vancouver, BC.


10. Russell V Anthony, Amelia R Tanner, Victoria C Kennedy, Quinton A Winger, Paul J Rozance. 9 Randel Lecture: In Vivo investigation of pregnancy Physiology Journal of Animal Science, Volume 102, Issue Supplement_1, March 2024, Pages 52–53.


11. Rogers H, Gad A, Cork G, Menjivar N, Schoolcraft WB, Tesfaye D, Yuan Y. Age-related transcriptomic profiling in human granulosa cells reveals mitochondrial insufficiency and microRNA regulation of key ovarian microenvironment dynamics. Fertility and Sterility 122:e168. (Poster Presentation) https://doi.org/10.1016/j.fertnstert.2024.07.587


12. Thompson RE, Gad A, Meyers MA, Menjivar NG, Tesfaye D, Hollinshead FK. Evaluation of extracellular vesicles secreted by mouse oviductal organoids for loading with CRISPR-cas9 ribonucleoproteins. Society for the Study of Reproduction 2024; 57th Annual Meeting. Dublin, Ireland. (Oral Presentation)


13. Thompson RE, Gad A, Meyers MA, Menjivar NG, Tesfaye D, Hollinshead FK. Extracellular Vesicles Secreted by Oviductal Organoids for Delivery of CRISPR-Cas9 Ribonucleoproteins. Rocky Mountain Reproductive Sciences Symposium 2024; 16th Annual Conference: Pages 54-55. Fort Collins, CO, USA. (Poster Presentation)


14. Wittenstein J, Gad A, Menjivar N, Tesfaye D. Strategies to Combating Oxidative Stress Damage During Bovine Embryo Development. Rocky Mountain Reproductive Sciences Symposium 2024; 16th Annual Conference: Page 42. Fort Collins, CO, USA. (Poster Presentation)


15. Ramírez GG, Gad A, Menjivar NG, Tesfaye D. Antioxidant-Based Strategies To Mitigate The Impact Of Heat Stress On Bovine Granulosa Cells And Oocytes. Rocky Mountain Reproductive Sciences Symposium 2024; 16th Annual Conference: Page 29. Fort Collins, CO, USA. (Poster Presentation)


16. Rogers H, Gad A, Cork G, Menjivar N, Schoolcraft WB, Tesfaye D, Yuan Y. Age-Related Transcriptomic Signatures in Human Granulosa Cells Emphasize Inflammation, Extracellular Matrix Remodeling, and Angiogenesis. Rocky Mountain Reproductive Sciences Symposium 2024; 16th Annual Conference: Page 17. Fort Collins, CO, USA. (Oral Presentation)


17. Gad A, Menjivar NG, Tesfaye D. The power of extracellular vesicles enhancing animal fertility. CSU Demo Day 2024; Annual Showcase. Fort Collins, CO, USA. (Poster Presentation)


18. Gad A, Menjivar NG, Tesfaye D. Fertility Resilience in the Face of Climate Change: The Role of Extracellular Vesicles. PASS Research Symposium 2024: Page 3-4. Fort Collins, CO, USA. (Oral Presentation)


19. Wittenstein J, Gad A, Menjivar N, Tesfaye D. Enhancing the antioxidant capacity of bovine embryos against oxidative stress using quercetin supplementation. CVMBS Research Day 2024: Page 120. Fort Collins, CO, USA. (Poster Presentation)


20. Rogers H, Gad A, Cork G, Menjivar N, Meyers M, Tesfaye D, Yuan Y. Transcriptomic insights into advanced maternal aged granulosa cells emphasizes inflammation and immune response. CVMBS Research Day 2024: Page 110. Fort Collins, CO, USA. (Poster Presentation).


21. Dunn B, Menjivar NG, Gad A, Thompson RE, Meyers MA, Hollinshead FK, Tesfaye D. Generation of physiologically relevant extracellular vesicles from bovine oviductal organoids. CVMBS Research Day 2024: Page 88. Fort Collins, CO, USA. (Poster Presentation).


22. Ramírez GG, Gad A, Menjivar NG, Tesfaye D. Enhancing the antioxidant capacity of granulosa cells to mitigate the impact of heat stress. CVMBS Research Day 2024: Page 46. Fort Collins, CO, USA. (Oral Presentation).


23. Braun H, Gad A, Barfield J. Time-Lapse Assessment of Bovine Embryos from Varying Oocyte Quality. Graduate Student Showcase 2024. Fort Collins, CO, USA. (Abstract / Poster Presentation)


24. Braun H, Gad A, Barfield J. Developmental potential and morphokinetics of in vitro produced bovine embryos generated from oocytes of varying quality. IETS 2025 Annual Conference. Fort Worth, TX, USA. (Abstract for Poster Presentation)


25. Braun H, Guzeloglu A, Bishop J, Georges H, Menjivar N, Marquez M, Hansen T, Barfield J. Identification and evaluation of transcriptomic markers for assessing bovine embryo quality from individual embryos or biopsies. CVBMS 26th Annual Research Day. Fort Collins, CO, USA. (Abstract for Oral Presentation)


26. Bakhtari A, Zhao R, Liu J, Su Y, Bielski K, and *Tian X. 2025. Ploidy correction during pre-implantation development of bovine triploid embryos. Poster at the 51st annual meeting of the International Embryo Technology Society, January 17-21, 2025, Fort Worth, TX, USA (published in Reproduction Fertility and Development, 2025.


27. Zhao R, Bakhari A, Liu J, *Tian X. 2025. Triploid Complementation: A Potential Alternative Approach to its Tetraploid Counterpart. Poster at the 51st annual meeting of the International Embryo Technology Society, January 17-21, 2025, Fort Worth, TX, USA (published in Reproduction Fertility and Development, 2025.


28. Losano JDA, Souders C, Binelli M, Martyniuk C, Daigneault BW. Beyond Motility - Advanced Bioenergetic Assessment of Bull Sperm. National Association of Animal Breeders 2024


29. Losano JDA, Guertin JE, McGraw M, Turner C, Pontes JHF, Fleury P, Maserati M, Callaham J, Bordignon V, Daigneault BW. Functional Capacitation of Stallion Sperm Promotes Interspecies Embryo Chimerism and Equine In Vitro Blastocyst Development from Cryopreserved Sperm. Society for the Study of Reproduction 2024.


30. Mohammad SS, Gauthier M, Martyniuk CJ, Kladde M, Daigneault BW. Postejaculatory Sperm Exposure to Tributyltin Chloride Induces Novel Structural, Genomic and Epigenomic Alterations in Mammalian Sperm. Society for the Study of Reproduction 2024.


31. Venturini M, Losano JDA, Heredia D, López M, McGraw M, Muhammad S, Guertin J, DiLorenzo N, Daigneault BW, Gonella A. Association between residual feed intake and reproductive-related traits in young bulls. American Society of Animal Sciences 2024.


32. Monzani, P.S., Sangalli, J.R., Sampaio, R.V., Guemra, S., Zanin, R., Adona, P.R., Berlingieri, M.A., Cunha Filho, L F.C., Mora-Ocampo, I.Y., Pirovani, C P., Meirelles, F.V., Ohashi, O. and Wheeler M.B. (2024). Human proinsulin and insulin production in the milk of transgenic cattle. Reproduction, Fertility and Development 36, (2) 231 https://doi.org/10.1071/RDv36n2Ab155


33. Marchioretto, P.V., Rodriguez-Zas, S L., Womack, S.A., Lindsey, B.R., Milner, D.J., Rubessa, M., Wilson, K.C. and Wheeler, M.B. (2024). Effect of dominant follicle removal before ovum pickup in Girolando cattle. Reproduction, Fertility and Development 36, (2) 251-252 https://doi.org/10.1071/RDv36n2Ab193


34. Glassey, J.R., Rabel, R.A.C., Milner, D.J. and Wheeler, M.B. (2024) Strontium enhances in vitro osteogenic differentiation of porcine adipocyte–derived stem cells. Reproduction, Fertility and Development 36, (2) 265-266 https://doi.org/10.1071/RDv36n2Ab220            


35. Bangert, E., Shipley, C., Rabel, R.A.C., Garrett, E., Milner, D. J., Marchioretto, P.V., Spencer, K., Allen, C., and Wheeler, M.B. (2024). The efficiency of an adapted bovine IVF protocol to produce in vitro-derived embryos from oocytes collected via surgical ovum pickup from live white-tailed deer (Odocoileus virginianus) donors under captivity in central Illinois. Reproduction, Fertility and Development 36, (2) 165-166 https://doi.org/10.1071/RDv36n2Ab32


36. Zimmerman, L.A., Bangert, E. A., Rabel, R. A C., Milner, D. J., Marchioretto, P.V., Allen, C.A. and Wheeler, M.B. (2024). The in vitro production of Gyr X Jersey bovine embryos from oocytes collected via ovum pickup for use in the tropics. Reproduction, Fertility and Development 36, (2) 155 https://doi.org/10.1071/RDv36n2Ab12                                        


37. Womack, S.A., Bethke, E.B., King, W P., Milner, D.J., Rubessa, M., Marchioretto, P.V., and Wheeler, M.B. (2024). Development of a porcine model for the testing of the RapidVent emergency ventilator for the treatment of COVID-19 infection. Reproduction, Fertility and Development 36, (2) 219 https://doi.org/10.1071/RDv36n2Ab132                                   


38. Wheeler, M.B. (2024). Awardee Talk - The Quest for an Ideal In-Field Embryo Evaluation Tool in Cattle: Opportunities and Challenges. Journal of Animal Science, Volume 102, Issue Supplement_3, September 2024, Pages 287–288, https://doi.org/10.1093/jas/skae234.329


39. Kim J, Yoon J, Chen J, Lee H, Redel B, Prather RS, Lee K. Enhancing specificity of gene editing outcomes by using Cas9 variants in swine embryos. IETS Jan 18 – 22. 2025.


40. Yoon J, Mony S, Kiesewetter E, Sullivan R, Kim J, Redel B, Uh K, Prather RS, Lee K. Effects of stem cell factor on the in vitro maturation of porcine oocytes and subsequent development following in vitro fertilization. IETS Jan 18 – 22. 2025.


41. Montgomery T, Uh K, Prather RS, Lee K. Identifying PRDM family members potentially involved in epigenetic reprogramming after fertilization. IETS Jan 18 – 22. 2025.


42. Li and J. Duan, 2024. Diversity of cow transposable elements and developmental expression in early embryos. Poster presentation at The Allied Genetics Conference (TAGC), Washington DC


43. Jingzhi Zhang*, Chia-Kuan Wu*, Meihong Shi, Abdallah Abdelhady, Yoke Lee Lee, Soon Hon Cheong†, Ellie Duan†. Profiling of H4K16 Acetyltransferase MOF in Bovine Embryogenesis. 2024 Poster presentation at Tri-Reproduction Symposium, Pittsburg, PA


44. Li, M. Shi, H. Araujo, A. Lee, J. Zhang, L. Lee, S. H. Cheong, J. E. Duan, 2024 Sex Differences of Bovine Blastocyst Revealed By Transcriptome Profiling and Long-Read Sequencing. To be presented as a poster presentation at Plant & Animal Genome 32, San Diego, CA


45. Li and Duan, J. E Comparative analysis of gene expression and transposable elements dynamics in bovine early embryos across different conditions. To be presented as a poster presentation at the 51st annual meeting of the International Embryo Technology Society, Fort Worth, TX


46. Shi, G.Li, H. Araujo, A. Lee, J. Zhang, L. Lee, S. H. Cheong, J. E. Duan, 2024 Transcriptomic difference of in vitro produced male and female early embryos in bovine. To be presented as a poster presentation at the 51st annual meeting of the International Embryo Technology Society, Fort Worth, TX


47. L. Murphy, A.K. Goldkamp, M.J.A. Lopes, N.F.F. Bonmann, M.C. Lucy, D.E. Hagen, J.G.N. Moraes. Alterations in miRNA and tRNA-derived fragment expression during bovine conceptus elongation. 2025 Annual Meeting of the International Embryo Technology Society (IETS), January 18-22, 2025, Fort Worth, Texas, USA.


48. Sellmer Ramos, M. O. Caldeira, S. E. Poock, Moraes, Joao G.N., Lucy, Matthew C, Patterson, Amanda L. Characterization of the endometrium epithelium of postpartum dairy cows using a multinomial transcriptomic approach to investigate disease programming of fertility. 21st Annual Gilbert S. Greenwald Symposium on Reproductive and Developmental Sciences, November 7-8, 2024, Kansas City, Kansas.


49. Roa-Vidal, Patricia, Markway, Macy, Moraes, Joao G.N., Lucy, Matthew C, Patterson, Amanda L. Uncovering the spatial gene expression of the bovine uterus: a novel study model for adenomyosis. 21st Annual Gilbert S. Greenwald Symposium on Reproductive and Developmental Sciences, November 7-8, 2024, Kansas City, Kansas.


50. J. Wilkinson, M.J.A. Lopes, G.L. Murphy, R.C. Botigelli, D. Londoño-Vásquez, Z. Jiang, A.C. Denicol, D.E. Hagen, J.G.N. Moraes. Establishment of protocols and conditions for culturing bovine embryonic stem cells. 2024 Poster Presentation of the Summer Research Training Program (SRTP) of the College of Veterinary Medicine, Oklahoma State University, August 8-10, Stillwater, OK.


51. F.F. Bönmann, L.G.D. Mendonça, I. Sellmer Ramos, R. Fritz, C. Gamarra, D. Duhatschek, R.S.S. de Oliveira, A.L.A. Scanavez, T.S. Belem, M.C. Lucy, J.G.N. Moraes. Behavioral and physiological differences of dry dairy cattle under heat stress based on median core body temperature. The 2024 ADSA Annual Meeting is scheduled for June 16-19, 2024, in West Palm Beach, Florida.


52. Van Rhijn S., Murphy G.L., Goldkamp A., Lopes M.J.A., Bonmann N., Hagen D., Moraes J.G.N. Transcriptional Regulation of Bovine Conceptus Elongation. 2024 OSU Undergraduate Research Symposium, April 16th, 2024, Oklahoma State University, USA.


53. Buckmaster, K. L., Melton, C., Lopes, M.J.A., Lamberti G.S.E., Briggs E. A., Lalman D.L., Moraes J.G.N. The Role of Interferon-Stimulated Genes in Early Recognition of Pregnancy in Cattle. Wentz and Purdie Research Scholars Symposium, April 25th, 2024, Oklahoma State University, USA.


54. Buckmaster, K. L., Melton, C., Lopes, M.J.A., Lamberti G.S.E., Briggs E. A., Lalman D.L., Moraes J.G.N. The Role of Interferon-Stimulated Genes in Early Recognition of Pregnancy in Cattle. 2024 Research Day at the Capitol, Oklahoma State University, March 18-19, 2024, Oklahoma City, Oklahoma, USA.


55. H. McDonald, C. P. Johnson, T. P. Vining, M. D. Major, G.S.E. Lamberti, M.I.A. Lopes, N.F.F. Bonmann, J.G.N. Moraes, and P. A. Beck. Effects of sire selection on puberty and reproduction of offspring selected as replacement heifers. 2024 American Society of Animal Sciences (ASAS) Southern Section Meeting, January 27-30, 2024, Louisville, Kenticky, USA.


56. Lamberti G.S., Goldkamp A.K., Lopes, M.J.A., Bonmann, N.F. F., Van Rhijn S., Hagen, D.E., Moraes, JGN. Unraveling the biology of conceptus elongation in cattle. 2024 American Society of Animal Sciences (ASAS) Southern Section Meeting, January 27-30, 2024, Louisville, Kentucky, USA.


57. Culler H, Zarzaca I, Mueller V, Elgarresta C, Hersom MJ, Checura CM. Effect of photobiomodulation in blastocysts post-vitrification. Reproduction, Fertility and Development 2025: 37 (1) 41. https://doi.org/10.1071/RDv37n1Ab41


58. Elgarresta C, Mueller V, Zarzaca I, Culler H, Duckett SK, Klotz JL, Hersom MJ, Checura CM. Vasoconstriction induced by ergot alkaloids affects oocyte developmental capacity in pregnant sheep. Reproduction, Fertility and Development 2025: 37 (1) 195. https://doi.org/10.1071/RDv37n1Ab195


59. Elgarresta C, Kordus R, Erck S, Fusco A, Strawbridge EG, Watson R, Checura CM. The Effect of Different Spermatozoa Preparation Techniques on DNA Fragmentation and the Presence of Reactive Oxygen Species. Clemson University 8th Annual Summer CI + UR Showcase, Clemson, SC. 2024: 24.


60. Klotz JL, Duckett SK, Checura CM. Serotonin stimulates relaxation in ovine saphenous vein precontracted with ergovaline. American Society of Animal Sciences Annual Meeting, Calgary, AB, Canada: 2024: 226. https://doi.org/10.1093/jas/skae234.324


61. Elgarresta C, Mueller V, Zarzaca I, Culler H, Hersom M, Checura CM. The Central Role of Mitochondria in In Vitro Fertilization. Clemson University 19th Annual Focus on Creative Inquiry Forum, Clemson, SC. 2024: 190.


62. Culler H, Richey KD, Checura CM. Photobiomodulation during oocyte maturation enhances blastocyst rates but not blastocyst adenosine triphosphate content. Reproduction, Fertility and Development 2024: 36 (2) 259. https://doi.org/10.1071/RDv36n2Ab208


63. Thomason L, Wilhelm C, Mandel A, Kirkman K, Richey KD, Culler H, Hersom M, Checura CM. Photobiomodulation of bovine zygotes at 21 hours postfertilization. Reproduction, Fertility and Development, 2024: 36 (2), 197. https://doi.org/10.1071/RDv36n2Ab91


64. Luense, LJ Plant and Animal Genomics, San Diego, CA, January 12^th, 2024, Investigating the Effects of an Abnormal Sperm Histone Epigenome on Preimplantation Embryo Development


65. R. Dodd, L.J. Luense. Investigating the presence of acetylated histone post-translational modifications in bovine preimplantation embryos. Society for the Study of Reproduction 2024.


66. J. Luense, G. Donahue, M. Mendoza, N. Leu, B. Garcia, M.S. Bartolomei, S.L. Berger, Abnormal sperm histone signature leads to altered preimplantation embryogenesis. Gordon Research Conference – Mammalian Reproduction, Barcelona, Spain, July 2024


67. R. Dodd, L.J. Luense. Investigating the presence of acetylated histone post-translational modifications in bovine preimplantation embryos. Texas A&M University Interdisciplinary Faculty of Reproductive Biology Retreat, Nov 2024


68. Adams, L., Liu, Y., Patrick, T., Grow, E., Ruggeri, E., Durrant, B. Polejaeva, I.A.* (2024). Quantification of mitochondrial DNA copy number in interspecies somatic cell nuclear transfer embryos. (2nd ed., vol. 35, pp. 136-136). Reproduction, Fertility and Development. 36:158-159.


69. Bennett, K., Beal, D., Liu, Y., Patrick, T., Bunderson, I., Moawad, A., Polejaeva, I.A.* (2024). Effects of oxygen level on bovine oocyte maturation and embryo development. Reproduction, Fertility and Development. 36:257.


70. Blocher, R., Liu, Y., Polejaeva, I.A.* (2024). Nuclear assessment of bovine oocytes matured in cytokine-supplemented medium using microtubule and DNA staining. Reproduction, Fertility and Development. 36:262.


71. Bunderson, I., Liu, Y., Polejaeva, I. A.* (2024). Effects of serum-free maturation medium and resveratrol supplementation on ovine oocyte maturation and quality. Reproduction, Fertility and Development. 36:263.

Miscellaneous publications (semi-technical/lay publications)

1. Looney, Charles R. (2024).  Culling beef cows for profit.  Arkansas Cattle Business. November 2024.


2. Looney, Charles R., Schowe, Clayton. (2024).  Using Beef Genetics for Dairy Calf Production Increases Sustainability.  Dairy E-News


3. Jiang Z. METTL7A improves embryo competence. 2024 Sep 24. U.S provisional patent application 63/698,174.


4. Jiang Z, Ming H. Methods and compositions for generating bovine extraembryonic endoderm cells and blastoids. 2024 Dec 16. US provisional patent application 63/734,491.


5. US Patent Number. 12,067,712B2 M.B. Wheeler et al. Date of Patent Issued :Aug. 20, 2024. Title: Phase Imaging with Computational Specificity” Filed: February 18, 2020. Application: Assessment for viability of sperm and embryos via artificial intelligence neural networks.

 

 

03/25/2026

W-5171 Multi-State Research Project

Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock

PUBLICATIONS

Washington – Publications

None

Utah – Publications

1. Refereed articles/book chapters

(* indicates the corresponding and/or senior author, italic font indicates contribution by an undergraduate student, and italic underlined font indicates contribution by a graduate student or postdoctoral fellow).

1. Leir, S-H., Tkachenko, S., Paranjapye, A., Meckler, F., Van Wettere, A.J., Kerschner, J.L., Viotti Perisse, I., Marriott C.M., Patrick, T., Liu, Y., White, K.L.,  Polejaeva, I.A., Harris, A.* (2025) Cellular imbalance in proximal and distal lung of CFTR^−/− sheep in utero and at birth Molecular Medicine  31:231.


2. Meckler, F., Van Wettere, A.J., Umrigar A., Bebek G., Leir S-H, Viotti Perisse, I., Lovrenert, K., White, K.L., Polejaeva, I.A., Harris, A.*. (2025) Molecular phenotypes in early gallbladder and liver disease in the CFTR^−/− sheep highlight aspects of cystic fibrosis-relevant hepatobiliary disease. Physiological Genomics. 57:682-694.

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

Renata Blocher. PhD. Dissertation title: Approaches to improve in vitro oocyte maturation and somatic cell nuclear transfer embryo developmental potential

Ian Bunderson. MS. Thesis title: Effect of resveratrol and commercial serum-free media on ovine oocyte quality and cloned embryo development

Jada Hansen. MS. Thesis title: Development and optimization of ovine in vitro fertilization for enhanced embryo development

1. Abstracts


2. Trowbridge, V., Blocher, R., Patrick, T., Perisse, IV., Marriott, C., Mustafa, Y., Miller, K., Polejaeva, I.A., Liu, Y.* (2025). Effect of cytoplasmic maturation and CPEB1 mRNA injection on DNA methylation patterns in bovine IVF embryos. Reproduction, Fertility and Development. 38:


3. Liu, Y., Grow, E., Viotti Perisse, I., Patrick, T., Thomas, A., Stott, R., Cairns, B., Polejaeva, I.A., White, K.L.* Impact of Early Biopsy and Chromatin Accessibility on the Development of Cloned Embryos in cattle. Proceeding of Society for the Study of Reproduction Annual meeting.


4. Miller, K., Hanson, J., Patrick, T., Liu, Y., Thomas, A., Perisse, I.V., Stott, R., Marriott, C., Blocher, R., Trowbridge, V., Polejaeva I.A,, White K.L.* (2025). Effects of serum and commercial serum-free medium on development of in vitro-produced (IVP) embryos in cattle Reproduction, Fertility and Development. 38:


5. Stott, R., Liu, Y., Marriot, C., Patrick, T., Blocher, R., Regouski, M., Perisse I.V., Polejaeva, I.A.* (2025). Effect of parturition induction methods on delivery of cloned lambs. Cinical Theriogenology. 17:44.


6. Miscellaneous publications (semi-technical/lay publications)

Texas - Publications

1. Refereed articles/book chapters

Ing, N.H., Curley Jr., K.O., Welsh Jr., T.O., Johnson, L., Luense, L.J., Clement, T.M. “Anatomy and Physiology of the Male Reproductive System and Potential Targets of Toxicants.” In Comprehensive Toxicology, 3^rd Edition, Elsevier Science, 2025

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

Dodd, A.R. Mapping histone acetylation throughout bovine spermatogenesis and preimplantation embryo development. Master’s Thesis, May 2025

1. Abstracts

Luense, L.J. The role of the paternal histone epigenome in preimplantation embryo development, International Embryo Technology Society, Fort Worth, TX, January 22, 2025

Dodd, A.R., Luense, L.J., Mapping histone acetylation throughout bovine spermatogenesis and preimplantation embryo development, Texas Forum for Reproductive Sciences, April 2025, Houston, TX

1. Miscellaneous publications (semi-technical/lay publications)

South Carolina – Publications

1. Refereed articles/book chapters 

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

1. Abstracts

Vogler, I., Summersett, S., Mueller, V., Zarzaca, I., Culler, H., Hersom, M.J., Checura, C.M. Photobiomodulation of Bovine Embryos at 72 Hours Post-fertilization. 38th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Salvador do Bahia, Brazil. Anim Reprod, 22 (3):96, 2025.

Mueller, V., Gainey, L., Gambardella, T., Summersett, S., Vogler, I., Elgarresta, C., Culler, H., Hersom, M., Kordus, R., Checura, C. In Vitro Fertilization In Different Species. Poster presentation at Clemson University 20th Annual Focus on Creative Inquiry Forum, Clemson, SC, USA. 2025. 

Elgarresta, C., Fusco, A., Strawbridge, E., Wang, Y., Checura, C., & Kordus, R. The Effects of Different Sperm Preparation Techniques on Reactive Oxygen Species and DNA Fragmentation. 27th Annual College of Reproductive Biology Symposium, Las Vegas, NV, USA. 2025.

1. Miscellaneous publications (semi-technical/lay publications)

Oklahoma - Publications

1. Refereed articles/book chapters

1. G.N. Moraes, T. Gull, A.C. Ericsson, M.O. Caldeira, T.J. Evans, S.E. Poock, M.C. Lucy. Evaluating differences in uterine microbiome and inflammatory status at 1 month postpartum associated with metritis and antibiotic treatment. Journal of Dairy Science. 2025 Sep 18, https://doi.org/10.3168/jds.2025-26403.

1. Amorim, S.T., Stafuzza, N.B., Cifuentes, D.C., Moraes, J.G.N., Dos Reis, B.R., Messmann, R., Camaripano, L. and Baldi, F. Genomic Study for Pregnancy Loss in Brahman Cattle. Journal of animal science, 2025, DOI: 1093/jas/skaf326

1. O. Caldeira, J.G.N. Moraes, T.T. Nguyen, J.C.C. Silva, I. Sellmer Ramos, S.E. Poock, T.E. Spencer, and M.C. Lucy. Impact of early postpartum metritis and systemic antibiotic treatment on the biochemistry and morphology of the bovine uterus at one month postpartum. Biol Reprod. 2025. DOI: 10.1093/biolre/ioaf146 

1. McDonald K, Drum JN, Moraes JGN, Melton C, Ojeda-Rojas OA, Venturini M, Gonella-Diaza AM, Prather RS, Ortega MS. Effect of FGF2, LIF, IGF1 supplementation on pregnancy success following embryo transfer of in vitro derived embryos. Theriogenology. 2025 Jun 10:117533. DOI: 1016/j.theriogenology.2025.117533

1. Caldeira MO, McDonald KS, Martinez ES, Moraes JGN, Ramos IS, Poock SE, Ortega MS, Lucy MC. Short-and long-term effects of uterine disease on oocyte developmental capacity in postpartum dairy cows. Theriogenology. 2025 Jul 1;240:117413.

1. Kiser JN, Seabury CM, Neupane M, Moraes JGN, Herrick AL, Dalton J, Burns GW, Spencer TE, Neibergs HL. Validation of loci and genes associated with fertility in Holstein cows using gene-set enrichment analysis-SNP and genotype-by-sequencing. BMC genomics. 2025 Feb 21;26(1):174.

1. Sellmer Ramos, M. O. Caldeira, S. E. Poock, J.G.N. Moraes, M. C. Lucy, and A.L. Patterson. Adenomyosis and fibrosis define the morphological memory of the postpartum uterus of dairy cows previously exposed to metritis. JDS Communications. 2025 doi.org/10.3168/jdsc.2024-0633

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

MS Thesis: 2

1. Lopes, M. J. A. 2025. Derivation and characterization of hybrid bovine × bison embryonic stem cell lines. M.S. Thesis. Oklahoma State Univ., Stillwater, OK.

1. Bonmann, N. F. F. 2025. CRISPR-Cas9-mediated alpha-Gal knockout in porcine fetal fibroblast cells. M.S. Thesis. Oklahoma State Univ., Stillwater, OK.

1. Abstracts

1. Bönmann N.F. F., lamberti, G. S. E., De Moraes, G. F., Lopes, M. J. A., Smith, R. M., Lucas, C.G., Moraes, J.G.N. CRISPR-Cas9-Mediated Alpha-Gal Knockout in Porcine Fetal Fibroblast Cells. 2025 Totusek Lectureship, November 21st, 2025, Oklahoma State University, Stillwater, Oklahoma.

1. Lopes MJA, Murphy GL, Wilkinson JJ, Bonnman NFF, Botigelli RC, Londono-Vasquez D, Denicol AC, Hagen DE, Moraes JGN. 2025. Derivation and Characterization of Hybrid Bovine x Bison Embryonic Stem Cells. A Closer Look. AETA 2025 Annual Convention.

1. Murphy G. L., Goldkamp A. K., Lopes M. J. A., Bonmann N. F. F., Lucy M. C., Hagen D. E., Moraes J. G. N. 2025. Let-7 microRNAs: Potential key modulators of bovine preimplantation development. 2025 AFS Research Day.

1. Murphy G. L., Goldkamp A. K., Lopes M. J. A., Bonmann N. F. F., Lucy M. C., Hagen D. E., Moraes J. G. N. 2025. Alterations in miRNA and tRNA-derived fragment (tRF) expression during bovine conceptus elongation. FAPC Research Symposium. 

1. Smith R.M., Bönmann N.F. F., de Moraes G. F., Lucas C.G., Moraes J.G.N. CRISPR-Cas9-Mediated Alpha-Gal Knockout in Porcine Fetal Fibroblast Cells. 2025 Freshman Research Scholars Symposium, April 17^th, 2025, Oklahoma State University, Stillwater, Oklahoma.

1. Snow M., Lopes M. J. A., Wilkinson. J. J, Murphy G. L., Botigelli R. C., Londoño-Vásquez D., Denicol A. C, Hagen D. E., Moraes J.G.N. Bovine-bison stem cell validation through embryoid body formation. 2025 Freshman Research Scholars Symposium, April 17^th, 2025, Oklahoma State University, Stillwater, Oklahoma.

1. Wilkinson, J., M.J.A. Lopes, G.L. Murphy, R.C. Botigelli, D. Londono-Vasquez, Z. Jiang, A.C. Denicol, D.E. Hagen, and J.G.N. Moraes. Establishment and validation of novel embryonic stem cell lines derived from Bison blastocysts. 2025, 28th Annual Phi Zeta Research Day. Oklahoma State College of Veterinary Medicine. Stillwater, OK. April 3, 2025.

1. Wilkinson, J., Lopes, M. J. A., Murphy, G. L., Botigelli, R. C., Denicol, A. C., Hagen, D. E., & Moraes, J. G. N. 2025. Establishment and validation of novel embryonic stem cell lines derived from hybrid bovine and bison blastocysts. AAVMC Veterinary Scholars Symposium, Spokane, WA. Poster Presentation

1. G.L. Murphy, A.K. Goldkamp, M.J.A. Lopes, N.F.F. Bonmann, M.C. Lucy, D.E. Hagen, J.G.N. Moraes. Alterations in miRNA and tRNA-derived fragment expression during bovine conceptus elongation. 2025. Annual Meeting of the International Embryo Technology Society (IETS), January 18-22, 2025, Fort Worth, Texas, USA.

1. Miscellaneous publications (semi-technical/lay publications)

New York - Publications

1. Refereed articles/book chapters

1. Fang, Y. Su, R. Meisel, J. Walters, T. Gamble, E Randolph, X. Yu, M. Shi, G. Li, J. Zhang, IISAGE Consortium, X.C. Tian, Y. Tang, J.E. Duan, Comparative Transcriptomic Analysis of Embryonic Stem Cells (ESCs) across Mammalian Species. bioRxiv 2025.03.11.642582; doi: https://doi.org/10.1101/2025.03.11.642582 iScience


2. Su,Y., Zhao, R., Fang, Y., Li, G., Jin, L., Liu, J., Zhu, J., Mishra, N., Kaback, D., Yee, S., Lei, C., Duan, J.*, Tian, X.*, Tang, T.*  (2025) Stem Cell. Bovine Formative Embryonic Stem Cells Capable of Three-Germ Layer and Extraembryonic Differentiation.


3. Shi, M., Li, G., Araujo, H.M. et al. Sex-biased transcriptome in in vitro produced bovine early embryos. Cell Biosci 15, 123 (2025). https://doi.org/10.1186/s13578-025-01459-x


4. Yu X., Harman R., Danev N., Li G., Fang Y., Van de Walle G., Duan J.E*. Under revision. Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells. Physiological Genomics, 2025


5. R.Biga*, J. E. Duan*, T E. Young*, J. R. Marks*, A. Bronikowski3*,L. P. Decena3*, E. C. Randolph1*, A. G. Pavuluri*, G. Li*, Y. Fang*, G. S. Wilkinson*, G. Singh#, N. T. Nigrin6#, E. N. Larschan6#, A. J. Lonski#, Nicole C. Riddle1^, and IISAGE Consortium (2024). Hallmarks of Aging: A User’s Guide for Comparative Biologists. 2024


6. Li G*, Danev, N*, Duan, J*, Van de Walle G. (2024), Comparative transcriptomic analysis of bovine mesenchymal 1 stromal cells reveals tissue-source and species-specific differences. iScience


7. Abdallah Abdelhady*, Jingzhi Zhang*, Chia-Kuan Wu, Yoke Lee Lee, Meihong Shi, Patrick L. Crane, Mariana Diel de Amorim, Ian R. Porter, Robert E. Thorne*, Jingyue (Ellie) Duan*, Soon Hon Cheong*. Cryopreservation impact the embryo transcriptomics and epigenomics. R1 in Scientific Reports. 2025


8. Aguilera J., Duan J., Lee S., Ray M., Larschan E. The CLAMP GA-binding transcription factor regulates heat stress-induced transcriptional repression by associating with 3D loop anchors. bioRxiv 2025.10.08.561401; doi: https://doi.org/10.1101/2023.10.08.561401

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations 

1. Duan et al., Bioinformatics Pipelines for the Integration Initiative: Sex, Aging, Genomics & Evolution (IISAGE)" Genomics and Epigenomics of Aging Workshop for the Plant and Animal Genome Conference (PAG)


2. Randolph., Y. Fang et al Defining Ubiquitous and Sex-Biased Aging Biomarkers Using Transcriptional Data across Evolutionarily Diverse Model and Non-Model Organisms, Plant and Animal Genome Conference (PAG)


3. Fang et al., Comparative Single-Cell Analysis of Epigenetic Regulator Dynamics during Early Gonadal Development, Plant and Animal Genome Conference (PAG)


4. Li, G., Shi, M.,. et al. Building the Embryonic Isoform Atlas in Bovine Preimplantation Embryos, Plant and Animal Genome Conference (PAG)

1. Abstracts

1. Animal Epigenome Reprogramming: Developmental Dynamics and Emerging Tools

Yifei Fang 1,* , Guangsheng Li 1,* , Meihong Shi 1,* , Xingtan (Vera) Yu 1,* , Jingzhi Zhang 1,* , and Jingyue (Ellie) Duan 1,§, Advances in Animal Genomics (Editor: S. Mondal and R. L. Singh). 

1. Miscellaneous publications (semi-technical/lay publications)

None

Nebraska - Publications

Montana - Publications

Arner, E.S.J., Schmidt, E.E., 2024. Unresolved questions regarding cellular cysteine sources and their possible relationships to ferroptosis. Advances in Cancer Research, 162, 1-44.

Borbényi-Galambos, K., Erdélyi, K., Ditrói, T., Jurányi, E.P., Szántó, N., Szatmári, R., Czikora, Á., Schmidt, E.E., Garai, D., Cserepes, M., Liszkay, G., Tóth, E., Tóvári, J., Nagy, P., 2025. Realigned Transsulfuration Drives BRAF V600E-Targeted Therapy Resistance in Melanoma. Cell Metabolism, in press.

Martí-Andrés, P., Finamor, I., Torres-Cuevas, I., Pérez, S., Rius-Pérez, S., Colino-Lage, H., Guerrero-Gómez, D., Morato, E., Marina, A., Michalska, P., León, R., Cheng, Q., Jurányi, E.P., Borbényi-Galambos, K., Millán, I., Nagy, P., Miranda-Vizuete, A., Schmidt, E.E., Martínez-Ruiz, A., Arnér, E.S., Sastre, J., 2024. TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. The EMBO Journal, 43, 2789-2812.

Schmidt, E.E., Miller, C.G., Juranyi, E.P., Austad, S.N., Ditroi, T., Seaford, Z.M., Yoon, S.J., Noyd, R.C., Kang, Y.P., Prigge, J.R., Ciskos, V., Kovari, D., DeNicola, G.M., Nagy, P., 2025. Cystine C-S bond cleavage fuels cysteine production under disulfide reductase deficiency. Nature Chemical Biology, in revision.

1. Refereed articles/book chapters

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations 

1. Abstracts 

1. Miscellaneous publications (semi-technical/lay publications)

Missouri - Publications

Sponchiado M., W.Gu, D.M.C. Madrid, R.A. Carroll, K.M. Davenport, C.G. Lucas, R.M. Sullivan, K.D. Wells, J.P. Driver, W.C. Warren, R.S. Prather, R.D. Geisert. 2025. Programming of the endometrial immune milieu by Interferon Gamma during Early Pregnancy in Pigs. Biol Reprod. ioaf221. doi: 10.1093/biolre/ioaf22. Selected as the Editors’ Choice

Eitel E.K., M. Sponchiado, R.M. Sullivan, C.G. Lucas, B.K. Redel, P.R. Chen, K.D. Wells, R.S. Prather, W.C. Warren, and R.D. Geisert. 2025. Expression of Tripartite Motif Family-Like Protein 1 and 2 in Early Conceptus Development and Placentation During Pregnancy in the Pig. Reprod Fert 6:e240107. doi: 10.1530/RAF-24-0107. Selected as the Editors’ Choice

Speckhart SL, Pollock AB, Alward KJ, Farrell K, Oliver MA, Lee K, Biase FH, Ealy AD. The interleukin-6 signal transducer receptor subunit is required for optimal in vitro bovine embryo development. Biol Reprod. 2025 Jan 5:ioaf006. doi: 10.1093/biolre/ioaf006. Online ahead of print.

Noland RS, Redel BK, LaMartina MG, Prather RS, Chen PR. Transcriptional profiling reveals upregulation of p53 signaling in porcine embryos produced in vitro. Biol Reprod. 2025 Oct 14;113(4):777-786. doi: 10.1093/biolre/ioaf113.

Zhang J, Bai H, Kawahara M, Balboula AZ, Takahashi M. (2025). Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle. Cell Tissue Res. 402(1):65-78.

Chasi B, Andrade JP, Monteiro PLJ, Castro B, Moreno E, Anta E, Ali NN, Cole JB, Wiltbank, Peñagaricano F, Balboula AZ, Ortega MS MC. (2025). Association of fertility traits with markers of oocyte competence in dairy cattle, J Dairy Sci. 108(11):12814-12823. 

1. Refereed articles/book chapters

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

1. Abstracts


2. Soto-Moreno and A.Z. Balboula, Spindle-localized F-actin regulates the fidelity of meiotic spindle formation, Fertilization and Activation of Development Gordon Research Conference, Holderness, New Hampshire, USA, 27 July – 1 August 2025 (TALK) 

1. Soto-Moreno and A.Z. Balboula, Spindle-localized F-actin regulates the fidelity of meiotic spindle formation, Fertilization and Activation of Development Gordon Research Seminar, Holderness, New Hampshire, USA, 26 July – 27 July 2025 (TALK)

Mangiavacchi P, Lee K, Redel BK. The Combination of CCL14 Chemokine and FLI During In Vitro Maturation Enhances In Vitro Development of Bovine Embryos. Society for the Study of Reproduction conference 2025, July 29 - August 1.

Nix J, Huang K, Kiesewetter E, Green C, Montgomery T, Samuel M, Lee K. Cryoprotective Treatment and Vitrification Stimulate the Oocyte Activation Cascade in Swine. Society for the Study of Reproduction conference 2025, July 29 - August 1.

Lee J, Yoon J, Miller L, Kim J, Redel B, Lee K. Canthaxanthin Reduces Polyspermy During In Vitro Fertilization in Pigs. Society for the Study of Reproduction conference 2025, July 29 – August 1.

Montgomery T, York M, Lee K. Identifying Lineage-Specific Transcripts in Different Embryonic Layers of Porcine Conceptuses During Peri-Implantation Development. Society for the Study of Reproduction Conference 2025, July 29 - August 1.

Green C, Jaworski A, Lee K, Prather RS, Redel B. Transcriptional profiling of cumulus cells from FGF, LIF, and IGF1 matured oocytes identifies junctional and structural pathways as key components in oocyte maturation. IETS Jan 18 – 22. 2025.

Kim J, Yoon J, Chen J, Lee H, Redel B, Prather RS, Lee K. Enhancing specificity of gene editing outcomes by using Cas9 variants in swine embryos. IETS Jan 18 – 22. 2025.

Yoon J, Mony S, Kiesewetter E, Sullivan R, Kim J, Redel B, Uh K, Prather RS, Lee K. Effects of stem cell factor on the in vitro maturation of porcine oocytes and subsequent development following in vitro fertilization. IETS Jan 18 – 22. 2025.

Montgomery T, Uh K, Prather RS, Lee K. Identifying PRDM family members potentially involved in epigenetic reprogramming after fertilization. IETS Jan 18 – 22. 2025.

1. Miscellaneous publications (semi-technical/lay publications)

Mississippi - Publications

1. Refereed articles/book chapters 

• Dlamini NH, Bridi A, da Silveira JC, Feugang JM. Unlocking Gamete Quality Through Extracellular Vesicles: Emerging Perspectives. Biology. Feb 13;14(2):198. 2025 - https://doi.org/10.3390/biology14020198

• Oladejo EO, Gruhot TR, Park S, Ishak GM, Mote BE, Liao SF, Feugang JM. Dietary Arginine Supplementation Modulates the Proteome of Boar Seminal Plasma. Animals. 2025 Feb 14;15(4):555. https://doi.org/10.3390/ani15040555

• Dlamini NH, Santos-Rivera M, Vance-Kouba CK, Pechanova O, Pechan T, Feugang JM. Integrating Near-Infrared Spectroscopy and Proteomics for Semen Quality Biosensing. Biosensors. 2025 Jul 15;15(7):456. https://doi.org/10.3390/bios15070456 

• Dlamini NH, Kameni SL, Feugang JM. Untargeted Metabolomic Identifies Potential Seasonal Biomarkers of Semen Quality in Duroc Boars. Biology. 2025 Aug 4;14(8):995. https://doi.org/10.3390/biology14080995

• Kameni SL, Dlamini NH, Feugang JM. Differential Metabolomic Signatures in Boar Sperm with Varying Liquid Preservation Capacities at 17° C. Animals. 2025 Jul 22;15(15):2163. https://doi.org/10.3390/ani15152163

• Beg MA, Ismail AO, Alaiya A, Khan FA, Hamoda TA, Sheikh IA, Sharma P, Baothman OM, Alkhzaim AH, Shinwari Z, Abuzinadah RF, Mohammed A, Assiri AM, Abuzenadah AM, Memili E, Feugang JM. Age-Associated Proteomic Changes in Human Spermatozoa. International Journal of Molecular Sciences. 2025 Jun 25;26(13):6099. https://doi.org/10.3390/ijms26136099

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

• Feugang JM, Othman OE, Nandolo W, Kugonza DR, Osei-Amponsah R. Prospect for Utilization of Modern Technologies for Small Ruminant and Pig Improvement in African Input Systems: Case Studies. In African Livestock Genetic Resources and Sustainable Breeding Strategies: Unlocking a Treasure Trove and Guide for Improved Productivity 2025 Nov 1 (pp. 1049-1068). Cham: Springer Nature Switzerland. 2025 - https://doi.org/10.1007/978-3-031-92076-9_24

• Feugang JM, Osei-Amponsah R, Rege JE, Fantazi K, Tiambo CK, Shumbusho F, Houaga I, Harek D, Dlamini NH, Gaouar SB. Conservation and management of animal genetic resources in the context of African livestock production systems: The case for in situ and ex situ conservation. In African Livestock Genetic Resources and Sustainable Breeding Strategies: Unlocking a Treasure Trove and Guide for Improved Productivity 2025 Nov 1 (pp. 1071-1089). Cham: Springer Nature Switzerland. 2025 - https://doi.org/10.1007/978-3-031-92076-9_25


• Ibeagha-Awemu EM, Peters SO, Bemji MN, Feugang JM, Osei-Amponsah R, Trocmé D, Mrode R. Role of modern technologies for sustainable genetic improvement of African livestock. In African Livestock Genetic Resources and Sustainable Breeding Strategies: Unlocking a Treasure Trove and Guide for Improved Productivity 2025 Nov 1 (pp. 851-908). Cham: Springer Nature Switzerland. 2025 - https://doi.org/10.1007/978-3-031-92076-9_20

1. Abstracts

• Mills M, Dlamini NH, Kameni SL, Maynard J, Brister T, Daous HE, Rajput S, Sidelinger DR, King HH, Feugang JM, Lemley CO. 41 Impact of oral melatonin supplementation on sperm quality and testicular artery hemodynamics in the bull. Journal of Animal Science. 2025 Jun;103(Supplement_2):136-. https://doi.org/10.1093/jas/skaf170.155

• Dlamini NH, Kameni SL, Feugang JM. 31 Metabolomic analysis of boar seminal plasma identifies sperm-quality related metabolites. Journal of Animal Science. 2025 Jun;103(Supplement_2):139-. https://doi.org/10.1093/jas/skaf170.158

• Kameni SL, Dlamini NH, Feugang JM. 87 Metabolomic insights into preserved boar semen: Differential profiles in high-survival and low-survival semen during liquid storage. Journal of Animal Science. 2025 Jun;103(Supplement_2):49-. https://doi.org/10.1093/jas/skaf170.061

Louisiana - Publications

1. Refereed articles.

Brewer, A.*, Girka, E.*, Dalton, A., Gutierrez-Castillo, E., and K. R. Bondioli. 2025. Bovine oocyte vitrification and recovery with ethylene glycol and either propylene glycol or dimethyl sulfoxide. Theriogenology. 9:242:117439.

1. Abstracts

Gutierrez-CastilloA, E. A. Hoang, F. Diaz, S. Dawn and K. Bondioli, R. 2026. Exploration of the cryoprotective properties of carboxylated poly-L-lysine for vitrification of IVM bovine oocytes. Reprod. Fert. Dev. 38:19.

 

Iowa - Publications

Illinois - Publications

1. Refereed articles/book chapters

1). Wang, Geng, Janet E. Sorrells, Rishyashring R. Iyer, Jindou Shi, Carlos A. Renteria, Edita Aksamitiene, Eric J. Chaney, Jennifer Mitchell, R.A.C. “Chanaka” Rabel, Derek J. Milner, Matthew B. Wheeler, Martha U. Gillette, Stephen A. Boppart, Haohua Tu. Label-free super-multiplex multiphoton imaging microscopy. Proceedings Volume PC13311, Optical Biopsy XXIII: Toward Real-Time Spectroscopic Imaging and Diagnosis; PC133110Q (2025) https://doi.org/10.1117/12.3040967
Event: BiOS, 2025, San Francisco, California, United States.

2). Womack , S.A., Bethke, E., King, W.P., Milner, D.J., Rubessa, M., Marchioretto, P.V., Shipley, C.F., Wheeler, M.B., Development of a Porcine Model for the Testing of the RapidVent Emergency Ventilator for the Treatment of COVID-19. J Reg Med Biol Res. 2025;6(2):1-9. https://doi.org/10.46889/JRMBR.2025.6204

3). Marchioretto, Paula V., Sandra L. Rodriguez-Zas, Brad R. Lindsey, and Matthew B. Wheeler. Effect of Follicle Ablation Before Ovum Pick-Up in Girolando Cattle Journal of Scientific Agriculture 2025, 9: 1-5. https://doi.org/10.25081/jsa.2025.v9.9602

4). Bangert, E. A.  and M. B. Wheeler. Assisted Reproductive Technologies and Genetic Improvement Strategies for Enhancing Livestock Production and Food Security in Tropical East Africa: A Review. Journal of Scientific Agriculture 2025, 9: 1-15 https://doi.org/10.25081/jsa.2025.v9.9599

5). Bionaz M., Jensen, T.W., Monaco, E., Maki, A.J., Rabel, R. A. C., Wheeler, M. B. In-vitro Osteogenic Capacity and In-vivo Bone Healing of Porcine Freshly Isolated Adipose-Derived Stem Cells (ASC), CS34+ ASC and Bone Marrow-Derived Stem Cells. J Reg Med Biol Res. 2025;6(3):1-18. https://doi.org/10.46889/JRMBR.2025.6306

6). Kanzawa, S., O’Meara, E., Funnell, B. J.,  Bangert, E.A., Garrett, E.F., Cardoso, F. C., Braz, C. U.,  Wheeler, M. B. The Effects of Heat Stress on the Population of the Ovarian Follicles and in vitro Embryo Production in Dairy Cows. Theriogenology (published online December 23, 2025) Volume 253, 15 March 2026, 117790, https://doi.org/10.1016/j.theriogenology.2025.117790

7). Gweniviere Capron, Parinaz Fathi, Tor Wolf Jensen, Derek J. Milner, André J. van der Vlies, Regan L. Moody, Elizabeth A. Bangert, Blair Rowitz, Dipanjan Pan, and Matthew B. Wheeler. 3D-Printed Gastrointestinal Stents: In Vivo Evaluation In a Swine Small Bowel Perforation Model. Advanced Healthcare Materials (2025): e05169. https://doi.org/10.1002/adhm.202505169 First published: 26 December 2025.

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

N/A

1. Abstracts

1).  De Oliveira AT, Bangert B, Rubessa M, Milner D, Okonkwo J, Ponnuraj N, Sivaguru M, Wheeler MB. (2026) Mapping transcript distribution in in vitro bovine embryos using spatial transcriptomics. Reproduction Fertility and Development 38, RDv38n1Ab189. https://doi.org/10.1071/RDv38n1Ab189

2).  De Oliveira AT, Rubessa M, Ulanov A, Scott B, Wheeler MB. (2026) Forever chemical: industrial contaminant detection across US bovine embryos. Reproduction Fertility and Development 38, RDv38n1Ab90. https://doi.org/10.1071/RDv38n1Ab90

3).  De Oliveira AT, Rubessa M, Milner D, Berbel AM, Ponnuraj N, Sivaguru M, Wheeler MB. (2026) Spatial transcriptomics of in vivo–produced bovine embryos: transcript density in three distinct embryonic cell lineages. Reproduction Fertility and Development 38, RDv38n1Ab190. https://doi.org/10.1071/RDv38n1Ab190

4).  De Oliveria AT, Braz C, Zimmerman L, Stewart K, Scott B, Wheeler MB. (2026) Comparative analysis of semen separation methods for in vitro fertilization in goats. Reproduction Fertility and Development 38, RDv38n1Ab104. https://doi.org/10.1071/RDv38n1Ab104

5).  Bangert EA, Lindsey BR, Rubessa M, Newman JK, Naves LM, Marchioretto PV, Wheeler MB. (2026) Improving bovine embryo transfer outcomes through precise alignment of embryo development with recipient estrous cycles. Reproduction Fertility and Development 38, RDv38n1Ab94. https://doi.org/10.1071/RDv38n1Ab94

6).  Zimmerman L, De Oliveira AT, Rubessa M, Ulanov A, Scott B, Wheeler MB. (2026) Forever chemical: industrial contaminant detection between in vitro- and in vivo-produced embryos. Reproduction Fertility and Development 38, RDv38n1Ab91. https://doi.org/10.1071/RDv38n1Ab91

7).  Nasser L, Bangert EA, Braz CU, Jimenez R, Torres ME, Wheeler MB. (2026) Use of GnRH versus P4 as an exogenous hormonal support for corpus lutea to improve embryo transfer pregnancy rates in crossbred recipients under a tropical environment. Reproduction Fertility and Development 38, RDv38n1Ab8. https://doi.org/10.1071/RDv38n1Ab8

8).  Wheeler, M.B., ASAS Randel Lecture: Bovine Embryo Evaluation—From Optics to Omics: My Journey, June 2025, Journal of Animal Science 103(Supplement 2):48-49, DOI: 10.1093/jas/skaf170.060 

1. Miscellaneous publications (semi-technical/lay publications)

US Patent Number. 12,067,712B2 M.B. Wheeler et al. Date of Patent Issued :Aug. 20, 2025. Title: Phase Imaging with Computational Specificity” Filed: February 18, 2020. Application: Assessment for viability of sperm and embryos via artificial intelligence neural networks.

Florida - Publications

1. Referred articles

1. Jiang, Z. (2025). Blastoids for modeling early embryonic development: application to domestic livestock. Annual Review of Animal Biosciences. https://doi.org/10.1146/annurev-animal-111523-102012


2. Jiang, Z. (2025). Molecular factors driving the development of bovine embryos and embryo-like structures. Animal Reproduction, 22(3). https://doi.org/10.1590/1984-3143-AR2025-0056


3. Ming H, Iyyappan R, Kakavand K, Dvoran M, Susor A, Jiang Z#. Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development. Nucleic Acids Research, 53(18). https://doi.org/10.1093/nar/gkaf956


4. Ming, H., Scatolin, G. N., Ojeda-Rojas, O. A., & Jiang, Z. (. (2025). Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation. Cell Reports, 44(6), 115707. https://doi.org/10.1016/J.CELREP.2025.115707


5. Zhu, L., Ming, H., Scatolin, G. N., Xiao, A., & Jiang, Z. (. (2025). METTL7A improves bovine IVF embryo competence by attenuating oxidative stress. Biology of Reproduction, 112(4), 628-639. https://doi.org/10.1093/BIOLRE/IOAF018.


6. Siddique MS, Anand S, Gauthier MPL, Brant JO, Kladde MP, Bhandari RK, Daigneault BW. Tributyltin chloride alters the structural, genomic, and epigenomic integrity of postejaculatory mammalian sperm. Epigenetics. 2025. Ahead of Press: http://dx.doi.org/10.1080/15592294.2025.2552129.


7. Mallory J. Llewellyn, Muhammad S. Siddique, Emma Ivantsova, Bradford Daigneault, Tracie R. Baker, Christopher J. Martyniuk. Lead as a reproductive endocrine disruptor in aquatic species and agricultural livestock. Pollutants. 2025. 5(3), 28; https://doi.org/10.3390/pollutants5030028


8. Losano JDA, Ren J, Yue F, Daigneault BW. Implementation of high-resolution respirometry for modeling bull fertility. J Animal Sci. 2025 Jan 4:103:skaf209. doi: 10.1093/jas/skaf209.


9. Mauro Venturini, Joao Agostini de Losano, Daniella C Heredia, Maria C Lopez Duarte, Maura McGraw, Sohail Muhammad, Jillian Guertin, Nicolas Dilorenzo, Bradford W Daigneault, Angela M Gonella-Diaza. Association between residual feed intake and reproductive-related traits in young bulls. J Animal Sci. 2025 https://doi.org/10.1093/jas/skaf055.


10. Guertin JE, Losano JDA, Salazar S, Callaham J, Daigneault, BW. Prolonged maintenance of cooled stallion semen by optimization of storage conditions. Journal of Equine Veterinary Sciences. 2025 Jan;144:105243. doi: 10.1016/j.jevs.2024.105243.

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

    Abstracts

1. Siddique MS, Anand S, Bhandari RK, Daigneault BW. Recreational levels of THC exposure from cannabis elicits structural, genomic and novel epigenomic changes to mature sperm that impact embryo development. Society for the Study of Reproduction 2025.


2. Siddique MS, Ananad S, Gauthier M, Martyniuk M, Kladde M, Bhandari R, Daigneault BW. Mature Mammalian Sperm are Suseptible to Novel Epigenetic Modifications and Structural Damage from Environmental Toxins. Society for the Stud of Reproduction 2025.

1. Miscellaneous

Invited Speaker for the Dairy Podcast Show - Daigneault, B. W. (2025). Improving Reproductive Performance - Bull Fertility Evaluation. The Dairy Podcast Show. https://youtu.be/U4-23_HUxrA?si=4NWzVPMQEwZt1Bdx

Connecticut - Publications

1. Refereed articles/book chapters: (*=correspondence)

1) Zhao R, Liu J, Bakhtari A, Shen J, Fayad-Costa A, Wang B, and Tian* X. 2025. Evidence against the role of toll-like receptors 7 and 8 in sex selection in mice, cattle, and humans. iScience 28, 113164; https://doi.org/10.1016/j.isci.2025.113164

2) Fang Y, Su Y, Meisel R, Walters JR, Gamble T, Randolph EC, Yu X, Shi  M, Li G, Zhang J, IISAGE Consortium, Tian* XC, Tang* Y, Duan JE. 2026. Comparative transcriptomic analysis of embryonic stem cells across mammalian species.  iScience 29, 114542; https://doi.org/10.1016/j.isci.2025.114542

3) Ranjitkar S, Shiri M, Sun J, Tian* X. 2025. Poly(A)-Selected Intergenic Transcripts in In Vivo Developed Bovine Oocytes and Pre-Implantation Embryos. Mol Reprod Dev 2025 Feb;92(2):e70017; https://doi.org/10.1002/mrd.70017

4) Su Y, Zhao R, Fang Y,Renxiu M, Li G, Jin L, Liu J, Yang Z, Li N,Zhu J, Mishra N, Kaback D, Yee SP, Luo Y, Wan W, Zhao Y, Wang X, Han S, Xiao P, Lei C, Duan* JE, Tang* Y, Tian* XC. 2026. Bovine formative embryonic stem cell plasticity in embryonic and extraembryonic differentiation. Stem Cells. Jan 8;44(1):sxaf068. doi: 10.1093/stmcls/sxaf068.

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations: N/A

1. Abstracts

1) Bielski K, Zhao R, Bakhtari A, Liu J, Guan Z, Tian X. 2026. Presented at the IETS Annual Conference in Panama City, Panama (abstract #188). Multipolar Mitosis as a Self-Correction Mechanism for Polyploidy in Pre-implantation Mouse Embryos

1. Miscellaneous publications (semi-technical/lay publications): N/A

Colorado - Publications

1. Refereed articles/book chapters

Publication (Winger lab)

Omar AK, Puma LCL, Risk BD, Witt AC, Izon CS, Whitcomb LA, Kareng DJ, Winger QA, Bouma GJ, Chicco AJ. Validation of an ovine model for studying impacts of prenatal docosahexaenoic acid supplementation on fetal tissue membrane composition and metabolism. J Nutr Biochem. 2025 Oct 4;148:110137. doi: 10.1016

Publication (Barfield lab)

Guzeloglu, A., Bishop, J. V., Georges, H. M., Braun, H. E., Menjivar, N., Marquez, M. A., Hansen, T. R., & Barfield, J. P. (2025). Identification of transcriptome markers associated with in vitro bovine embryo quality in single embryos and biopsies†. Biology of reproduction, ioaf260. Advance online publication. https://doi.org/10.1093/biolre/ioaf260

Lugo, E. A., Graham, J. K., Gillis, J. D., & Barfield, J. P. (2026). Investigating the plasma membrane composition and osmotic tolerance of domestic felid (Felis catus) spermatozoa as a model for non-domestic felids. Animal reproduction science, 285, 108076. https://doi.org/10.1016/j.anireprosci.2025.108076

Publication (Tesfaye lab)

Nico G Menjivar, Ahmed Gad, Riley E Thompson, Mindy A Meyers, Soham Ghosh, Fiona K Hollinshead, Dawit Tesfaye 2025. Organoids simulating the bovine oviduct mediate the embryo-maternal interface via extracellular vesicle-transmitted signaling, Human Reproduction Open, hoaf076, https://doi.org/10.1093/hropen/hoaf076

Heather M Rogers, Ahmed Gad, Gentry K Cork, Nico G Menjivar, William B Schoolcraft, Dawit Tesfaye, Ye Yuan 2025. Age-related integrative transcriptomic profiling of human granulosa cells reveals mRNA-microRNA regulatory network associated with key ovulation dynamics. Biology of Reproduction, f034, https://doi.org/10.1093/biolre/ioaf034

E Held-Hoelker, J Kurzella, C Benedetti, H Habermann, F Rings, E Tholen, D Tesfaye, M Hoelker. 2025. The mitochondrial bioenergetic profile in response to L-Carnitine as a clue for vitality of in vitro produced bovine embryos cultured under serum enriched conditions. Theriogenology, Volume 251,117746, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2025.117746

1. Rahimi, M. Schreiber, H. Habermann, L. Haake, D. Miskel, D. Tesfaye, E. Held-Hoelker, B. Brenig, C. Benedetti, M. Hoelker. 2025. Efficient genome editing of bovine in vitro derived zygotes via Cas9 RNP-electroporation using extended-stored bovine ovaries, Theriogenology, Volume 250, 2026, 117703, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2025.117703.

Luca Angi Souza, Nico G. Menjivar, Ahmed Gad, Paulo Henrique Groppo Rodrigues, Leticia Rabello da Silva Sousa, Paola Maria da Silva Rosa, Alessandra Bridi, Dawit Tesfaye, Juliano Coelho da Silveira 2026: Small extracellular vesicles from follicular fluid as transport carriers of LHR: possible mediators of follicle growth and dominance acquisition in bovine reproduction. Anim Reprod. 2026;23(1):e20250051 | https://doi.org/10.1590/1984-3143-AR2025-0051

Dessie Salilew-Wondim, Ernst Tholen, Christine Große-Brinkhaus, Eva Held-Hoelker, Dennis Miskel, Franca Rings, Karl Schellander, Urban Besenfelder, Vitezslav Havlicek, Dawit Tesfaye & Michael Hoelker 2025. Sexually dimorphic gene expression responses of bovine embryos to the maternal microenvironment on day 13 of gestation. BMC Genomics 26, 372. https://doi.org/10.1186/s12864-025-11570-5

Dennis Miskel, Jessica Kurzella, Franca Rings, Ernst Tholen, Dawit Tesfaye, Karl Schellander, Dessie Salilew-Wondim, Eva Held-Hoelker, Christine Große-Brinkhaus, Michael Hoelker 2025. Functional COPA is indispensable for early embryo development beyond major genome activation in bovines. Theriogenology, 241 (117415). https://doi.org/10.1016/j.theriogenology.2025.117415

1. Schreiber, C. Benedetti, M. Rahimi, H. Habermann, F. Rings, E. Tholen, D. Tesfaye, M. Hoelker, E. Held-Hoelker. 2025. Tauroursodeoxycholic acid (TUDCA) improves cryo-resilience and lowers energy turnover via affecting the mitochondrial bioenergetic profile in bovine embryos, Theriogenology, Volume 249, 2026, 117632, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2025.117632.

Eva Held-Hoelker, Lientje Sophie Haake, Jessica Kurzella, Maibritt Schreiber, Christina Dauben, Dessie Salilew-Wondim, Nasser Ghanem, Franca Rings, Christine Große-Brinkhaus, Ernst Tholen, Dawit Tesfaye, Michael Hoelker (2025). Heat stress during maturation of bovine oocytes profoundly impacts the mitochondrial bioenergetic profile and causes endoplasmic reticulum stress in subsequent blastocysts, Biology of Reproduction, ioaf070, https://doi.org/10.1093/biolre/ioaf070

Rahman, M.B., Held-Hoelker, E., Saeed-Zidane, M. Rings, Franca, Salilew-Wondim, Dessie, Tesfaye, Dawit, Gad, Ahmed, Gebremedhn, Samuel, Tholen, Ernst, Schellander, Karl, Große-Brinkhaus, Christine, Hoelker, Michael. 2025. Comparative transcriptome analysis of bovine blastocysts reveals specific effects of the oocyte source and the environments during maturation and early embryo development. BMC Genomics 26, 673 (2025). https://doi.org/10.1186/s12864-025-11848-8

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertation 

Nico G. Menjivar​, Camila Azzolin de Souza​, Ahmed Gad, Luca A. Souza, Samuel Gebremedhn, Juliano C. da Silveira, Dawit Tesfaye. 2025. Strategies for Loading miRNA Cargo into Extracellular Vesicles. In Neuro-methods Series

1. Abstracts and Talks

Invited talks by PI (Barfield)

Barfield JP. Embryo Grading Webinar, Universidad Cientifica del Sur, Peru, March 2025

Barfield JP. Practical Considerations for Embryo Freezing, American Embryo Transfer Association, Reno, NV, Oct 2025

California - Publications

• Denicol AC, Botigelli RC. Dissecting the development of the early lineages and primordial germ cells in the bovine embryo. Anim Reprod. 2025 Oct 3;22(3):e20250098. doi: 10.1590/1984-3143-AR2025-0098. PMID: 41059287


• Candelaria JI, Botigelli RC, Guiltinan C, Shikanov A, Denicol AC. Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles. J Assist Reprod Genet. 2025 May;42(5):1509-1523. doi: 10.1007/s10815-025-03497-3. Epub 2025 May 20. PMID: 40392485


• Guiltinan C, Botigelli RC, Candelaria JI, Smith JM, Arcanjo RB, Denicol AC. Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics. Biol Open. 2025 Mar 15;14(3):BIO061819. doi: 10.1242/bio.061819. Epub 2025 Mar 7. PMID: 39957479

1. Refereed articles/book chapters

1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations 

1. Abstracts

Transition of embryonic stem cells to low oxygen conditions reveals changes associated with energetic metabolism without altering the pluripotency core. R. C. Botigelli, R. Braz-Arcanjo, C. Guiltinan, J. Smith and A. C. Denicol. [Conference abstract]. Reproduction, Fertility and Development 38, RDv38n1Ab1. doi:10.1071/RDv38n1Ab1

Embryonic genome transcription in cattle occurs as early as the 4-cell stage and involves mRNA processing factors, epigenetic regulators, and conserved transcription factors. M. D. Altman, A. C. Denicol, R. M. Schultz and P. J. Ross. [Conference abstract]. Reproduction, Fertility and Development 38, RDv38n1Ab1. doi:10.1071/RDv38n1Ab1

Evaluation of mechanical isolation techniques for efficient harvesting of bovine preantral follicles

1. A. Hyde, A. N. P. Morais, A. L. Conceição-Santos, L. Engelman, M. O. Gastal, B. Graceli, A. C. Denicol and E. L. Gastal [Conference abstract]. Reproduction, Fertility and Development 38, RDv38n1Ab1. doi:10.1071/RDv38n1Ab1

McDonnell SP, McKissick EE, Denicol AC. The Role of Connexin 37 in Early Antral Bovine Folliculogenesis. Abstract presented at the 2025 Annual Meeting of the Society for the Study of Reproduction, Washington DC, USA. 

1. Miscellaneous publications (semi-technical/lay publications)

Arkansas - Publications