02/07/2023

Peer-Reviewed Literature

 

Michigan State University

De Jong, E., K. D. McCubbin, D. F. Kelton, J. Sanchez, S. Dufour, S. McDougall, P.L. Ruegg, A. Lago, V. Kromker, S. M. Godden, T. J.G.M, Lam, P. J. Rajala-Schultz, J. R. Middleton, K. Orsel, J. P. Kastelic, and H. W. Barkema. 2022.  Selective treatment of non-severe clinical mastitis does not adversely affect cure, somatic cell count, milk yield, recurrence and culling:  a systematic review and meta-analysis. Available online:  https://doi.org/10.3168/jds.2022-22271

 

Rodriguez, Z., Q. Kolar, K. Krogstad, T. Swartz, I.Yoon,  B. Bradford and P. L. Ruegg.  Evaluation of reticuloruminal temperature for the prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. In press, J Dairy Sci, https://doi.org/10.3168/jds.2022-22421 

 

deCampos, J.L., J.M. Strickland, J. Gandy, C. Robison, and P.L. Ruegg. 2022.  A pilot randomized clinical trial evaluating clinical effects of pegbovigrastim administered to dairy cows at dry-off. submitted Am. J. Veterinary Research. Online early publication:  https://doi.org/10.2460/ajvr.22.08.0130

 

Gonçalves, J.L., J. Leite de Campos, A. Steinberger, N. Safdar, A. Sethi, J. Shutske, G. Suen, T. Goldberg, Roger I. Cue and P. L. Ruegg. 2022.  Incidence and treatments of mastitis and other selected bovine diseases on 37 large Wisconsin dairy farms. Pathogens 11:1282. https://doi.org/10.3390/pathogens11111282

 

Pegolo, S., R. Tessari, V. Bisutti, D. Giannuzzi, M. Gianesella, A. Lisuzzo, E. Fiore, A. Barberio, E. Schizvon, E. Trevisi, F. P. Capelli, L. Gallo, P. Ruegg, R. Negrini, and A. Cecchinato. 2022.  Quarter-level analyses of the associations between subclinical intramammary infection and milk quality, udder health and cheese-making traits

 University of Minnesota

 Brink, A.A., W. J. Weber, J. D. Lippolis, J. B. Cole, S. M. Godden, A. Seykora and B. A. Crooker. 2022. Effect of Holstein genotype on ex-vivo cytokine response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) during the periparturient period. Vet. Immunol. Immunopathol. https://doi.org/10.1016/j.vetimm.2022.110463

Lippolis, J. D., E. J. Putz, T. A. Reinhardt, E. Casas, W. J. Weber and B. A. Crooker. 2022. Effect of Holstein genotype on immune response to an intramammary Escherichia coli challenge. J. Dairy Sci. 2021-21166. https://doi.org/10.3168/jds.2021-21166

 

Dean CJ, Peña-Mosca F, Ray T, Heins BJ, Machado VS, Pinedo PJ, Caixeta LS, Noyes NR. 2022. Evaluation of Contamination in Milk Samples Pooled From Independently Collected Quarters Within a Laboratory Setting. Front Vet Sci. 2022 Jun 16;9:818778. https://doi.org/10.3389/fvets.2022.818778

Ray, T., T. Gaire, C. Dean, S.M. Rowe, S.M. Godden, N.R. Noyes. 2022. The microbiome of common bedding materials before and after use on commercial dairy farms. Animal Microbiome (2022) 4: 18 https://doi.org/10.1186/s42523-022-00171-2

 

University of Missouri

 Adkins PRF, Placheta L, Borchers M, Bewley J, Middleton JR.  2022.  Distribution of staphylococcal and mammaliicoccal species from compost-bedded pack or sand-bedded freestall dairy farms.  J Dairy Sci. 105(7):6261-6270.  [Epub ahead of print 13 May 22].  https://doi.org/10.3168/jds.2021-21500

McCubbin K, de Jong E, Lam T, Kelton D, Middleton JR, McDougall S, De Vliegher S, Godden S, Rajala-Schultz P, Rowe S, Speksnijder D, Kastelic J, Barkema, H.  2022.  Invited Review: Selective use of antimicrobials in dairy cattle at drying off.  J Dairy Sci.  105(9):7161-7189.  [Epub ahead of print 2 Aug 2022].  https://doi.org/10.3168/jds.2021-21455

Kurban D, Roy, JP, Kabera F, Frechette A, Um MM, Albaaj A, Rowe S, Godden S, Adkins PRF, Middleton JR, Gauthier ML, Keefe GP, DeVries TJ, Kelton DF, Moroni P, dos Santos MV, Barkema HW, Dufour S.  2022.  Diagnosing Intramammary Infection: Meta-analysis and Mapping Review on Frequency and Udder Health Relevance of Microorganism Species Isolated from Bovine Milk Samples.  Animals.  12(23):3288.  https://doi.org/10.3390/ani12233288

Loy JD, Clawson ML, Adkins PRF, Middleton JR.  2022.  Current and Emerging Diagnostic Approaches to Bacterial Pathogens of Ruminants.  Vet Clin North Am Food Anim Pract.  VFP_860.  Invited Article.  In Press

 

Cornell University

 Larsen J, Raisen CL, Ba X, Sadgrove NJ, Padilla-González GF, Simmonds MSJ, Loncaric I, Kerschner H, Apfalter P, Hartl R, Deplano A, Vandendriessche S,Černá Bolfíková B, Hulva P, Arendrup MC, Hare RK, Barnadas C, Stegger M, Sieber RN, Skov RL, Petersen A, Angen Ø, Rasmussen SL, Espinosa-Gongora C, Aarestrup FM, Lindholm LJ, Nykäsenoja SM, Laurent F, Becker K, Walther B, Kehrenberg C, Cuny C, Layer F, Werner G, Witte W, Stamm I, Moroni P, Jørgensen HJ, de Lencastre H, Cercenado E, García-Garrote F, Börjesson S, Hæggman S, Perreten V, Teale CJ, Waller AS, Pichon B, Curran MD, Ellington MJ, Welch JJ, Peacock SJ, Seilly DJ, Morgan FJE, Parkhill J, Hadjirin NF, Lindsay JA, Holden MTG, Edwards GF, Foster G, Paterson GK, Didelot X, Holmes MA, Harrison EM, Larsen AR. Emergence of methicillin resistance predates the clinical use of antibiotics. Nature. 2022 Feb;602(7895):135-141. doi: 10.1038/s41586-021-04265-w. Epub 2022, Jan 5. PMID: 34987223; PMCID: PMC8810379.

Riva F, Latorre AA, Moroni P. Editorial: Ruminant mastitis: A 360° view. Front Vet Sci. 2022 Oct 27;9:1055323. doi: 10.3389/fvets.2022.1055323. PMID: 36387386; PMCID: PMC9647156.

Preine F, Herrera D, Scherpenzeel C, Kalmus P, McCoy F, Smulski S, Rajala-Schultz P, Schmenger A, Moroni P, Krömker V. Different European Perspectives on the Treatment of Clinical Mastitis in Lactation. Antibiotics (Basel). 2022 Aug 16;11(8):1107. doi: 10.3390/antibiotics11081107. PMID: 36009976; PMCID:PMC9404852.

Kurban D, Roy JP, Kabera F, Fréchette A, Um MM, Albaaj A, Rowe S, Godden S, Adkins PRF, Middleton JR, Gauthier ML, Keefe GP, DeVries TJ, Kelton DF, Moroni P, Veiga Dos Santos M, Barkema HW, Dufour S. Diagnosing Intramammary Infection: Meta-Analysis and Mapping Review on Frequency and Udder Health Relevance of Microorganism Species Isolated from Bovine Milk Samples. Animals (Basel). 2022 Nov 25;12(23):3288. doi: 10.3390/ani12233288. PMID: 36496808; PMCID: PMC9738497.

Schell RC, Bulut E, Padda H, Safi AG, Moroni P, Ivanek R. Responsible antibiotic use labeling and consumers' willingness to buy and pay for fluid milk. J Dairy Sci. 2023 Jan;106(1):132-150. doi: 10.3168/jds.2022-21791. Epub 2022 Nov 1. PMID: 36333136.

Addis MF, Pisanu S, Monistero V, Gazzola A, Penati M, Filipe J, Di Mauro S, Cremonesi P, Castiglioni B, Moroni P, Pagnozzi D, Tola S, Piccinini R. Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence. Virulence. 2022 Dec;13(1):174-190. doi: 10.1080/21505594.2021.2024014. PMID: 35030987; PMCID: PMC8765078.

Dal Prà A, Biscarini F, Cavani GL, Bacchelli S, Iotti A, Borghi S, Nocetti M, Moroni P. Relationship between total and differential quarter somatic cell counts at dry-off and early lactation. PLoS One. 2022 Oct 17;17(10):e0275755. doi: 10.1371/journal.pone.0275755. PMID: 36251634; PMCID: PMC9576081.

Gioia G, Freeman J, Sipka A, Santisteban C, Wieland M, Gallardo VA, Monistero V, Scott JG, Moroni P. Pathogens associated with houseflies from different areas within a New York State dairy. JDS Commun. 2022 May 21;3(4):285-290. doi:10.3168/jdsc.2021-0200. PMID: 36338025; PMCID: PMC9623797.

Cremonesi P, Biscarini F, Castiglioni B, Sgoifo CA, Compiani R, Moroni P. Gut microbiome modifications over time when removing in-feed antibiotics from the prophylaxis of post-weaning diarrhea in piglets. PLoS One. 2022 Mar 7;17(3):e0262199. doi: 10.1371/journal.pone.0262199. PMID: 35255081; PMCID: PMC8901073.

Casseri E, Bulut E, Llanos Soto S, Wemette M, Stout A, Greiner Safi A, Lynch R, Moroni P, Ivanek R. Understanding Antibiotic Resistance as a Perceived Threat towards Dairy Cattle through Beliefs and Practices: A Survey-Based Study of Dairy Farmers. Antibiotics (Basel). 2022 Jul 25;11(8):997. doi: 10.3390/antibiotics11080997. PMID: 35892387; PMCID: PMC9330383.

 

Ohio State University

L.R. Larsen, L.E. Moraes, and B.D. Enger. 2022. Characteristics of mammary secretions collected from infected and uninfected primigravid dairy heifer mammary glands. J. Dairy Sci. 105:7615–7622. doi.org/10.3168/jds.2022-21794.

 University of Tennessee

Gelgie, A. E., Korsa, M. G. and Kerro Dego, O. 2022. Mycoplasma bovis Mastitis. Current Research in Microbial Sciences, 3, 100123. doi:10.1016/j.crmicr.2022.100123.

Gelalcha, B. D.and Kerro Dego, O. 2022. Extended-Spectrum Beta-Lactamases Producing Enterobacteriaceae in the USA Dairy Cattle Farms and Implications for Public Health, Antibiotics, volume 11, issue 10,13113, doi:10.3390/antibiotics11101313.

Gelalcha, B. D., Brown, S., Crocker, H., Agga, G. E. and Kerro Dego, O. 2022. Regulation Mechanisms of Virulence Genes in Enterohemorrhagic Escherichia coli. Foodborne Pathogens and Disease 19(9):598 -612. doi:10.1089/fpd.2021.0103.

 

Gelalcha, B. D., Ensermu, D. B., Agga, G. E., Vancuren, M., Gillespie, B. E., D'Souza, D. H., Okafor, C. C., Kerro Dego, O. 2022. Prevalence of Antimicrobial Resistant and Extended-Spectrum Beta-Lactamase-producing Escherichia coli in Dairy Cattle Farms in East Tennessee. Foodborne Pathog Dis., 19(6), doi:10.1089/fpd.2021.0101.

 

Vidlund, J., B. D. Gelalcha, S. Swanson, I. Fahrenholz, C. Deason, C. Downes and O. Kerro Dego. 2022. Pathogenesis, Diagnosis, Control, and Prevention of Bovine Staphylococcal Mastitis. In. IntechOpen. doi: 10.5772/intechopen.101596. Available at https://doi.org/10.5772/intechopen.101596.

 University of Vermont

 Luyi Jiang, Huizeng Sun, Fengfei Gu, Jin He, Fengqi Zhao, and Jianxin Liu. (2022). Blood neutrophil extracellular traps: A novel target for the assessment of mammary health in transition dairy cows. Journal of Animal Science and Biotechnology 13:131. Doi: 10.1186/s40104-022-00782-4

 

Lu-Yi Jiang, Hui-Zeng Sun, Ruo-Wei Guan, Fushan Shi, Feng-Qi Zhao, Jian-Xin Liu. (2022). Formation of blood neutrophil extracellular traps increases the mastitis risk of dairy cows during the transition period. Frontiers in Immunology, in press

 

Fengfei Gu, L. Y. Jiang, Diming Wang, Feng-Qi Zhao, Jianxin Liu. (2022). Supplementation with N-carbamoylglutamate during the transition period improves the functions of neutrophils and reduces inflammation and oxidative stress in dairy cows. Journal of Dairy Science. doi: 10.3168/jds.2021-21159

Neher DA, Andrews TD, Weicht TR, Hurd Ad, Barlow JW 2022. Organic Farm Bedded Pack System Microbiomes: A Case Study with Comparisons to Similar and Different Bedded Packs. Dairy 2022, 3, 587–607. DOI: 10.3390/dairy3030042

 

Virginia Tech.

Swartz, T.H. and C. S. Petersson-Wolfe. Associations between preweaning calf feeding behaviors with age at first calving and lactational performance using an automatic calf feeder.

J Dairy Sci. Comm. Dec 2022 -0255. doi: 10.3168/jdsc.2022-0255. Online ahead of print.

 

Swartz TH, Bryant DM, Schramm HH, Duncan AJ, White RR, Wood CM, Petersson-Wolfe CS. The effects of dexamethasone administration on physiological, behavioral, and production parameters in dairy cows after a difficult calving. J Dairy Sci. 2022 Nov 15:S0022-0302(22)00663-4. doi: 10.3168/jds.2022-22029. Online ahead of print.

 

Liebe DM, Steele NM, Petersson-Wolfe CS, De Vries A, White RR. Practical challenges and potential approaches to predicting low-incidence diseases on farm using individual cow data: A clinical mastitis example. J Dairy Sci. 2022 Mar;105(3):2369-2379. doi: 10.3168/jds.2021-20306. Epub 2022 Jan 25. PMID: 35086707

Abstracts

 

Michigan State University

Rodriguez, Z., Q.K. Kolar, K. Krogstad, I. Yoon, B. Bradford, and P.L. Ruegg.  2022.   Evaluation of the performance of an intra-reticuloruminal remote sensor for the prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. J Dairy Sci 105 supp 1:1408.

Kolar, Q.K., K. Krogstad, Z. Rodriguez, V. Mavangira, T. Swartz, I. Yoon, B. Bradford, and P.L. Ruegg. 2022.   Effect of supplementation with Saccharomyces cerevisiae fermentation product on udder health and milk yield after intramammary challenge with S. uberis.  J Dairy Sci 105 supp 1:2288W.

Kolar, Q.K., S.M. Godden, S.M., and P.L. Ruegg.  2022.  Outcomes of Treatment of Gram-positive Cases of Non-Severe Clinical Mastitis by Pathogen Group. Pp 246-247 in Proc. 61st Ann. Meeting NMC, San Diego, CA, Feb 1-3, 2022.

Goncalves, J.L., J. Leite de Campos, A. Steinberger, N. Safdar, A. Sethi, J. Shutske, Z. Rodriguez, G. Suen, T. Goldberg, and P.L. Ruegg.  2022.  Outcomes of first cases of selected bovine diseases on 37 large WI dairy farms. Pp 212-213 in Proc. 61st Ann. Meeting NMC, San Diego, CA, Feb 1-3, 2022.

Babiak, A.M., Q.K. Kolar, J. Leite de Campos, and P.L. Ruegg. 2022.  Accuracy of On-farm PCR Method Used for Identification of Selected Mastitis Pathogens. Pp 202-203 in Proc. 61st Ann. Meeting NMC, San Diego, CA, Feb 1-3, 2022.

 

University of Minnesota

Lippolis, J. D., E. J. Putz, T. A. Reinhardt, E. Casas, W. J. Weber, B. A. Crooker. 2022. Holstein genotype impacts immune response to an intramammary Escherichia coli challenge. Proc. 61st Annu. Conf. National Mastitis Council. February 3. San Diego, CA. virtual. NMC Proceedings Library. http://nmconline.omnibooksonline.com/

 

Lippolis, J. D., E. J. Putz, T. A. Reinhardt, E. Casas, W. J. Weber, B. A. Crooker. 2022. Whole blood transcript profiles in Holstein cows subjected to an intramammary Escherichia coli challenge. Proc. 61st Annu. Conf. National Mastitis Council. February 3. San Diego, CA. virtual. NMC Proceedings Library. http://nmconline.omnibooksonline.com/

 

Pena Mosca, F., Dean, C.J., Caixeta, L.S., Ray, T., Heins, B., Machado, V.S., Pinedo, P.J., Noyes, N.R. “Description of the intramammary infections dynamics in early lactation heifer on organic dairy farms.” 54th Annual Conference of the American Association of Bovine Practitioners. Salt Lake City, UT, October 2021.

Caixeta, L.S., Dow, S., Noyes, N.R., Crooker, B.A., Godden, S.M., Nydam, D.V., Walcheck, B., Wheat, W. “Effect of mucosal immune stimulant on mammary gland immune responses during dry and lactating periods in dairy cows.” Conference of Research Workers in Animal Diseases. Chicago, IL, December 2021.

Dean, C. Pena-Mosca, F., Fernandes, L., Sharpe, K., Manriquez, D., Doster, E., Antunes Jr, A.M., Calles, V.F., Bauman, C., Wehri, T., Heins, B., Pinedo, P., Machado, V.S., Caixeta , L.S., Noyes, N.R., “Longitudinal survey of the bovine teat microbiome.” Conference of Research Workers in Animal Diseases. Chicago, IL, December 2021.

Pena-Mosca, F., Dean, C., Ray, T., Heins, B., Pinedo, P., Machado, V.S., Caixeta, L.S., Noyes, N.R. “Association between bacterial group and persistence in the mammary gland in early lactation primiparous cows.” Conference of Research Workers in Animal Diseases. Chicago, IL, December 2021.

Florentino, C.C., Pena-Mosca, F., Ruch, M., Mahmoud, M.M., Tikofsky, L., Godden, S.M., Caixeta, L.S. “The effects of administration of acidogenic boluses at dry-off in udder health and milk yield in early lactation – interim results.” 61st Annual Meeting of the National Mastitis Council, San Diego, CA. February 2022.

Pena-Mosca, F., Florentino, C.C., Rocha, C., Feijoo, V.C., Masic, A., Borchers, M., Asper, D., Caixeta, L.S. “Investigation of the inclusion of on-farm measurement of somatic cell count and differential cell count in algorithms to predict cows with intramammary infections at dry-off.” 61st Annual Meeting of the National Mastitis Council, San Diego, CA. February 2022.

Rowe, S.M., Dziuba, M., Boyum, B., Godden, S.M., Royster, E., Caixeta, L.S. “Negatively controlled trial investigating the effect of dry cow therapy on clinical mastitis and culling.” 61st Annual Meeting of the National Mastitis Council, San Diego, CA. February 2022.

Pena Mosca, F., Florentino, C.C., Miranda, J., Rial, C., Laplacette, A., Masic, A., Borchers, M., Asper, D., Caixeta, L.S. “Assessment of the association between single somatic cell count measurement, milk culture and selective dry cow therapy algorithms.” ADSA Conference. Kansas City, MO, June 2022.

Florentino, C.C., Pena Mosca, F., Ruch, M., Tikofsky, L., Knauer, W., Cramer, G., Godden, S, M., Caixeta, L.S. “ Randomized clinical trial evaluating the effects of the administration of acidogenic boluses o at dry-off on udder health.” 55th Annual Conference of the American Association of Bovine Practitioners. Long Beach, Ca. September 2022.

Pena Mosca, F., Florentino, C.C., Miranda, J., Rial, C., Laplacette, A., Masic, A., Borchers, M., Asper, D., Caixeta, L.S. “Assessment of the relationship between differential somatic cell count and presence of subclinical mastitis.” 55th Annual Conference of the American Association of Bovine Practitioners. Long Beach, Ca. September 2022.

Pena Mosca, F., Florentino, C.C., Rial, C., Laplacette, A., Masic, A., Borchers, M., Asper, D., Caixeta, L.S. “Investigation of the relationship between differential somatic cell count and milk culture.” 55th Annual Conference of the American Association of Bovine Practitioners. Long Beach, Ca. September 2022

Godden, S., E. Royster, E. Leonard*, D. Albrecht, J. Timmerman. Pilot Study: Refining a Culture-Guided Selective Dry Cow Therapy Program to Enhance Antimicrobial Stewardship on Dairy Farms. 55^th Annu Mtg. AABP. Long Beach, CA, Sept 22-24, 2022.

 

Peña Mosca, F.*, S. Godden, E. Royster, D. Albrecht, B.A. Crooker, N. Aulik. Investigation of the Relationship between Manure Processing Method and Presence of M. avium subsp. paratuberculosis and Salmonella spp. in Recycled Manure Solids Bedding on Midwest Dairy Farms. 55^th Annu Mtg. AABP. Long Beach, CA, Sept 22-24, 2022.

 

Godden, S., F. Peña Mosca, E. Royster, D. Albrecht, B.A. Crooker. Investigation of the Relationship between Manure Processing Method and Levels of Mastitis Pathogens in Recycled Manure Solids Bedding on Midwest Dairy Farms. 55^th Annu Mtg. AABP. Long Beach, CA, Sept 22-24, 2022.

 

University of Missouri

Haw SR, Adkins PRF, Middleton JR. Characterization of intramammary infections in Jersey cows. Proceedings of the 61st Annual Meeting of the National Mastitis Council, San Diego CA, Feb 1-3, 2022.

 

Kurban D, Roy JP, Kabera F, Frechette A, Um MM, Albaaj A, Rowe S, Godden S, Adkins PRF, Middleton JR, Gauthier ML, Keefe G, DeVries TJ, Kelton DF Moroni P, dos Santos MV, Barkema HW, and Dufour S.  2022.  Diagnosing intramammary infections: meta-analysis and mapping review on frequency and udder health relevance of microorganism species retrieved in bovine milk samples. Proceedings of the 61st Annual Meeting of the National Mastitis Council, San Diego CA, Feb 1-3, 2022.

 

Haw S, Adkins PRF, Middleton JR, Bernier Gosselin V.  Pathogen-specific intramammary infection prevalence, persistence, and somatic cell count association in lactating Jersey cows.  Proceedings of the 44^th Annual CVM Research Day.  6 May 2022.  Abstract #52

 

Isensee P and Adkins P. Characterization of the antibacterial activity of Staphylococcus chromogenes isolates originating from dairy cattle. MU CVM Research Day. May 6, 2022

 

Kurban D, Roy JP, Kabera F, Frechette A, Um MM, Albaaj A, Rowe S, Godden S, Adkins PRF, Middleton JR, Gauthier ML, Keefe G, DeVries TJ, Kelton DF Moroni P, dos Santos MV, Barkema HW, and Dufour S.  2022.  Diagnosing intramammary infection: Meta-analysis and mapping review on frequency and udder-health relevance of microorganism species retrieved from bovine milk samples.  16^th International Symposium of Veterinary Epidemiology and Economics, Halifax Nova Scotia, Canada.  August 7-12, 2022.

 

Ohio State University

 

Baker, P.H., K.M. Enger, S.K. Jacobi, R.M. Akers, B.D. Enger. 2022. Effect of Staphylococcus aureus intramammary infection on heifer mammary gland growth and development. J. Dairy Sci. 105 (Suppl. 1):177.

Baker, P.H., K.M. Enger, S.K. Jacobi, R.M. Akers, B.D. Enger. 2022. Cellular proliferation in Staphylococcus aureus infected heifer mammary glands that were hormonally stimulated to rapidly grow. J. Dairy Sci. 105 (Suppl. 1):301.

Larsen, L.R., P.H. Baker, K.M. Enger, L.E. Moraes, and B.D. Enger. 2022. Prepartum administration of internal teat sealant in primigravid dairy heifers to reduce intramammary infection prevalence at calving. Pages 272-273 in Natl. Mastitis Counc. Annu. Mtg. Proc., San Diego, CA, Natl. Mastitis Council Inc., New Prague, MN.

Baker, P.H., F.K. Arnold, D.D. Clevenger, S.K. Jacobi, R.M Akers, and B.D. Enger. 2022. Histological response in hormonally stimulated mammary glands of non-pregnant dairy heifers to a Staphylococcus aureus intramammary infection. Pages 228-229 in Natl. Mastitis Counc. Annu. Mtg. Proc., San Diego, CA, Natl. Mastitis Council Inc., New Prague, MN.

 Oregon State University

Ford HR, Zheng T, Bionaz M, Huo Q, Hasan D. 2022. The D2Dx immunity test as a measure of immune health in ewes and lambs. J. Dairy Sci. Vol. 105:163 Suppl. 1

Ford HR, Bionaz M, Trevisi E, Hasan D. 2022. Feeding chicory silage to transition ewes has a minimal effect on blood metabolic parameters but a strong effect on blood micromineral levels. J. Dairy Sci. Vol. 105:157 Suppl. 1

Ford HR, Bionaz M, Ates S, Trevisi E. 2022. A combination of chicory-plantain silage and Se-yeast have a minimal effect on blood biomarkers during intramammary infection in lactating ewes. J. Dairy Sci. Vol. 105:109 Suppl. 1

 

University of Vermont

 

Heins K, Chakrawarti A, Eckstrom K, Mugabi R, Barlow JW 2022 Genetic Variation in The Type I Restriction-Modification Systems of Staphylococcus aureus from Selected Vermont Dairy Farm and their Association with Antimicrobial-Resistant Genes. ASM Microbe, American Society of Microbiology Joint Annual Meeting, June 9-13, Washington, DC.

Prandini SC, Jeffrey CE, Chakrawarti A, Barlow JW 2022. Case Report: Hock Lesions as a Source of Staphylococcus aureus Mastitis. Proceedings of National Mastitis Council, 61st Annual Meeting, San Diego CA.

Astmann TJ, Jeffrey CE, Chakrawarti A, Barlow JW 2022. Bacteria Isolated from Bovine Teat Skin Inhibit Staphylococcus aureus in an in vitro Simultaneous Antagonist Assay. Proceedings of National Mastitis Council, 61st Annual Meeting, San Diego CA.

Chakrawarti A, Eckstrom K, Laaguiby P, Mugabi R, Burke A, Barlow JW 2021. Concordances between whole genome sequencing and conventional methods for pathogen strain typing and antimicrobial resistance of Staphylococcus aureus isolated from humans, cattle and milk on cheese making dairy farms. World Microbe Forum, American Society of Microbiology Joint   Annual Meeting, June 20-24, virtual.

 

Conference Proceedings

 

Michigan State University

Ruegg, P.L. 2022. Microbiology, Maldi-Tof, Microbiome and more:  how to understand and use diagnostic tests to improve udder health. In Proc. Am Assoc Bov Pract

 

Ruegg, P.L. 2022. Treatment of clinical mastitis. What we know and where we go. Pp 62-71 in Proc. 61^st Ann. Meeting NMC, San Diego, CA, Feb 1-3, 2022.

University of Missouri

Adkins PRF. Molecular Diagnostics – The Good, The Bad, and The Ugly. Proceedings of the 61st Annual Meeting of the National Mastitis Council, San Diego CA, Feb 1-3, 2022.

University of Tennessee

Desai, S., Vidlund, J. and Kerro Dego, O. 2022. Evaluation of protective efficacy of novel staphylococcal mastitis vaccines in cattle. The 26^th Annual Exhibition of Undergraduate Research and Creative Achievement (EURēCA) Symposium, April 25, The University of Tennessee, Knoxville.

Gelgie, A. E., Gillespie, B. E., Almeida, R., Lysnyansky, I., Shpigel, N. Y., Agga, G. E. and Kerro Dego, O. 2023. Identification of virulence genes of Mycoplasma bovis by transposon mutation and experimental infection of dairy cows. Accepted for presentation at Conference of Research Workers on Animal Disease (CRWAD), January 20 -24, Chicago, IL.

 

Poster Presentations

 

Outreach Presentations

 

University of Minnesota

 

 

Caixeta, L.S. “Behind a successful lactation is a proper dry-off.”

• November 2021: Western Dairy Management Conference, Reno, NV


• March 2022: Central Plains Dairy Expo, Sioux Falls, SD

 

 

10/07/2024

 

Peer-Reviewed Literature

University of Minnesota

Peña-Mosca F; Dean CJ; Fernandes LM, Doster E, Sharpe K, Tui R, Feijoo V, Antunes A, Baumann C, Wehri T, Heins B, Pinedo P, Machado V, Noyes NR, Caixeta L. Associations between early lactation intramammary infections and udder health and performance during the first 180 days in milk in first-lactation organic dairy cows. Journal of Dairy Science. 2023. https://doi.org/10.3168/jds.2023-23924.

Mosca F, ^§Dean C, Machado V, Fernandes L, Pinedo P, Doster E, Heins B, Sharpe K, Baumann C, Ray T, Feijoo V, Antunes A, Wehri T, Noyes NR, Caixeta L. Investigation of intramammary infections in primiparous cows during early lactation on organic dairy farms. In press at Journal of Dairy Science. https://doi.org/10.3168/jds.2022-23036

Dean, C. , Y. Deng, T. Wehri, T. Ray, F. Peña-Mosca, B. Crooker, S. Godden, L. Caixeta, and N. Noyes. 2023. The impact of kit, environment and sampling contamination on the observed microbiome of bovine milk. mSystems https://doi.org/10.1101/2023.11.07.566052

Brink, A.A. , W.J. Weber, J.D. Lippolis, J.B. Cole, A.K. Rendahl, L. Caixeta, S.M. Godden, A.J. Seykora, and B.A. Crooker. 2023. Effect of Holstein genotype on ex-vivo interleukin-1β response to lipopolysaccharide (LPS), lipoteichoic acid (LTA) and heat-killed Gram-negative and Gram-positive
bacteria. Vet Imm & Immunopathology. 258:110573 https://doi.org/10.1016/j.vetimm.2023.110573

Florentino, C.C. , E. Shepley, M. Ruch, Mahmoud, M.M., L. Tikofsky, W. Knauer, G. Cramer, S. Godden, L. Caixeta. 2023. A randomized clinical trial evaluating the effects of acidogenic boluses administration at dry-off on rumination and activity behavior in the 14 subsequent days. Submitted to JDS Communications 4:293-297. https:// doi.org/10.3168/jdsc.2022-0366

Dean, C.J. , F. Peña-Mosca, T. Ray, T. J. Wehri, K. Sharpe, A.M. Antunes Jr., E. Doster, L. Fernandes, V. F. Calles, C. Bauman, S. Godden, B. Heins, P. Pinedo, V. S. Machado, L. S. Caixeta, N. R. Noyes. 2023. Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infection risk in primiparous cows under organic directives. BioRxiv https://doi.org/10.1101/2023.09.12.557435

Daneshi, M., Caton, J.S., Caixeta, L.S., Eftekhari, Z., Ward, A.K. “Expression, regulation, and function of β-defensins in the bovine mammary glands: current knowledge and future perspectives.” Animals. 2023, 13(21), 3372. https://doi.org/10.3390/ani13213372

Dziuba, M., L.S. Caixeta, B. Boyum, S. Godden, E. Royster, S. Rowe. 2023. Negatively controlled trial investigating the effect of dry cow therapy on clinical mastitis and culling in multiparous cows. J. Dairy Sci.  106:5687-5695

Rowe, S., F. Kabera, S. Dufour, S. Godden, J.P. Roy. 2023. Selective dry cow therapy can be implemented successfully in cows of all milk production levels. J. Dairy Sci. 106:1953-1967  https://doi.org/10.3168/jds.2022-22547

Godden, S., E. Royster, B.A. Crooker, J. Timmerman, F. Peña Mosca. 2023. Methods of processing recycled manure solids bedding on Midwest dairy farms I: Associations with bedding bacteria counts, milk quality, udder health and milk production. Bov. Pract. 57(1):10-20.

Brink, A.A., W.J. Weber, J.D. Lippolis, J.B. Cole, S.M. Godden, A. Seykora, B.A. Crooker. 2022. Effect of Holstein genotype on ex-vivo innate immune response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) during the periparturient period. J. Vet Immunology and Immunopathol. 251:110463.  https://doi.org/10.1016/j.vetimm.2022.11046

Ray, T., T. Gaire, C. Dean, S.M. Rowe, S.M. Godden, N.R. Noyes. 2022. The microbiome of common bedding materials before and after use on commercial dairy farms.  Animal Microbiome (2022) 4: 18 https://doi.org/10.1186/s42523-022-00171-2

Florentino, C.C. ^&; Pena Mosca, F.; Ruch, M.; Shepley, E.; Barbosa Arias, M.; Moreira, D.M.; Mahmoud, M.M.; Tikofsky, L.; Knauer, W.; Cramer, G.; Godden, S.; Caixeta, L.S. “Randomized clinical trial evaluating the effect of administering acidogenic boluses at dry-off on udder health and cow performance.” Journal of Dairy Science. 2024 . https://doi.org/10.3168/jds.2023-23757

Rutgers University; New Jersey Agricultural Experiment

Andrews T.P., Hoyer J.S., Fahrenfeld N.L., Boyd J.M.*, Duffy S.* Complete genome sequences of five Phietaviruses infecting Staphylococcus aureus. Microbiology Resource Announcements 10/2022 PMID: 36173192

Hudspeth, J.D., Boncella A.E., Sabo E.T., Andrews T., Boyd J.M., Morrison C.N., Structural and Biochemical Characterization of Staphylococcus aureus Cysteine Desulfurase Complex SufSU. American Chemical Society Omega. 11/2022 PMID: 36506149

Andrews, T., Ficken K., Fey P.D., Duffy S., Boyd J.M., Novel Transducing Bacteriophage Infecting Staphylococcus epidermidis Contributes to the Expansion of a Novel Siphovirus Genus and Implies Genus is Inappropriate for Phage Therapy. mSphere. 2/2023 PMID: 37017574

Ramírez-Hernández M., Norambuena J., Hu H., Thomas B., Tang C., Boyd J.M.*, Asefa T.* Repurposing Anthelmintics: Effects of Rafoxanide and Copper-Functionalized SBA-15 Carriers Against Methicillin-Resistant Staphylococcus aureus (MRSA). American Chemical Society Applied Materials & Interfaces 3/2023 PMID: 36975176

Norambuena J., Al-Tameemi H., Bovermann H., Kim J., Beavers W.N., Skaar E.P., Parker D., Boyd J.M. Copper ions inhibit pentose phosphate pathway function in Staphylococcus aureus. PLoS Pathogens. 5/2023 PMID: 37235600

Kim G.L., Kim J., Norambuena J., Boyd J.M., Parker D., Impact of the pentose phosphate pathway on metabolism and pathogenesis of Staphylococcus aureus. PLoS Pathogens. 6/2023 PMID: 37440594

Hossain S., Morey J.R., Neville S.L., Ganio K., Radin J.N., Norambuena J., Boyd J.M., McDevitt C.A., Kehl-Fie T.E., Host subversion of bacterial metallophore usage drives copper intoxication. mBio 8/2023 PMID: 37737591

Leanse L.G., dos Anjos C., Ryan Kaler K., Hui J., Boyd J.M., Hooper D.C., Anderson R.R., Dai T., Blue light potentiates antibiotic activity in bacteria via parallel pathways of hydroxyl radical production and enhanced antibiotic uptake. 8/2023 Advanced Science PMID: 37946633

University of Missouri

Kurban D, Roy JP, Kabera F, Fréchette A, Um MM, Albaaj A, Rowe S, Godden S, Adkins PRF, Middleton JR, Gauthier ML, Keefe GP, DeVries TJ, Kelton DF, Moroni P, dos Santos MV, Barkema HW, and Dufour S. 2022. Diagnosing intramammary infection: meta-analysis and mapping review on frequency and udder health relevance of microorganism species isolated from bovine milk samples. Animals. Nov 25; 12(23): 3288. https://doi.org/10.3390/ani12233288

de Jong EC, Creytens, L, De Vliegher S, McCubbin KD, Baptiste M, Leung AA, Speksnijder D, Dufour S, Middleton JR, Ruegg PL, Lam TJGM, Kelton DF, McDougall S, Godden SM, Lago A, Rajala-Schultz PJ, Orsel K, Kromker V, Kastelic JP, Barkema HW.  2023.  Selective treatment of nonsevere clinical mastitis does not adversely affect cure, somatic cell count, milk yield, recurrence, or culling: a systematic review and meta-analysis.  J Dairy Sci.  106(2):1267-1286.  https://doi.org/10.3168/jds.2022-22271

Loy JD, Clawson ML, Adkins PRF, Middleton JR.  2023.  Current and Emerging Diagnostic Approaches to Bacterial Diseases of Ruminants.  Vet Clin North Am Food Anim Pract.  39(1):93-114.  Invited Article.  https://doi.org/10.1016/j.cvfa.2022.10.006

de Jong E, McCubbin KD, Speksnijder D, Dufour S, Middleton JR, Ruegg PL, Lam TJGM, McDougall S, Godden SM, Lago A, Rajala-Schultz PJ, Orsel K, De Vliegher S, Kromker V, Kastelic JP, Barkema HW.  2023.  Invited Review: Selective treatment of clinical mastitis in dairy cattle.  J Dairy Sci.  106(6):3761-3778.  https://doi.org/10.3168/jds.2022-22826

Michigan State University

Kolar, Q., S. Godden, R. Erskine, and P.L. Ruegg.  2024.  SHORT COMMUNICATION:  Confirmed bacteriological diagnosis and clearance of non-severe Gram-positive clinical mastitis cases enrolled in a randomized clinical trial using an on-farm culture program.  J Dairy Sci Comm.  Uncorrected proof:  https://www.jdscommun.org/action/showPdf?pii=S2666-9102%2824%2900086-3

Strickland, J.M., J.L. de Campos, J. Gandy, V. Mavangira, P.L. Ruegg and L. Sordillo. 2024.  A randomized control trial to test the effect of pegbovigrastim treatment at dry off on plasma and milk oxylipid profiles during early mammary gland involution and post-parturient period.  J Dairy Science, available online:  https://doi.org/10.3168/jds.2023-23879

Vasco, K., S.Carbone, R.Mosci, B.Bowcutt, R. Colwell, K.Graubics, R.Erskine, B. Norby, P.L. Ruegg*, L. Zhang*, S.Manning*. 2023. Persistent effects of intramammary ceftiofur treatment on the gut microbiome and antibiotic resistance in dairy cattle.  Animal Microbiome.  5:56 (2023). https://doi.org/10.1186/s42523-023-00274-4  *co-corresponding authors

Pegolo, S., D. Giannuzzi, F. Piccioli-Cappelli, L. Cattaneo, M. Gianesella, P.L. Ruegg, E. Trevisi, and A. Cecchinato.  2023. Blood biochemical changes upon subclinical intramammary infection and inflammation in Holstein cattle.  J Dairy Sci.  106:6539-6550. https://doi.org/10.3168/jds.2022-23155

Pegolo, S., A. Toscanoa, V. Bisutti, A. Vanzin, D. Giannuzzi, L. Gallo, F. Tagliapietra,  P. Ruegg, M. Gianesella, M. Mele, S. Schiavon, and A. Cecchinato.  2023.  Associations between subclinical intramammary infection and milk fatty acid profile at the quarter level in Holstein cattle.  Animal 17(10), 100978.   https://doi.org/10.1016/j.animal.2023.100978

Goncalves, J.L., R. Mani, S. Sreevatsan, and P.L. Ruegg.  Apparent prevalence and selected risk factors of methicillin resistant and susceptible Staphylococcus aureus and non-aureus Staphylococci and Mammaliicocci in bulk tank milk of dairy herds in Indiana, Ohio, and Michigan.  2023.  J. Dairy Sci.Comm. available online:  https://doi.org/10.3168/jdsc.2023-0386

Leite de Campos, J., Goncalves, J.I., J., A. Kates, A. Steinberger, A. Sethi, G. Suen, J. Shutske, N. Safdar, T. Goldberg, and P.L. Ruegg.   2023.  Variation in Partial Direct Costs of Treating Clinical Mastitis among 37 Wisconsin Dairy Farms.  J Dairy Sci 106:9726-9286 https://doi.org/10.3168/jds.2023-23388 

South Dakota State University

Swartz, T. H., and C. S. Petersson-Wolfe. 2023. Short communication: Associations between pre-weaned calf feeding behaviors with age at first calving and lactational performance using an automatic calf feeder. J. Dairy Sci. Comm. 4:75-79. https://doi.org/10.3168/jdsc.2022-0255

Rodriguez, Z., Q. Kolar., K. Krogstad, T. H. Swartz, I. Yoon, B. J. Bradford, and P. Ruegg. 2023. Evaluation of a reticuloruminal temperature for the prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. J. Dairy Sci. 106:1360-1369. https://doi.org/10.3168/jds.2022-22421

Swartz, T. H., D. M. Bryant, H. H. Schramm, A. J. Duncan, R. R. White, C. M. Wood, and C. S. Petersson-Wolfe. 2023. The effects of dexamethasone administration on physiological, behavioral, and production responses in dairy cows after a difficult calving. J. Dairy Sci. 106:653-663. https://doi.org/10.3168/jds.2022-22029

Swartz, T. H., B. J. Bradford, M. Lemke, L. K. Mamedova, R. Agnew, J. Fehn, E. Owczarzak, J. L. McGill, and K. A. Estes. 2022. Effects of prenatal dietary rumen-protected choline supplementation during late gestation on calf growth, metabolism, and vaccine response. J. Dairy Sci. 105:9639-9651. https://doi.org/10.3168/jds.2022-22239

University of Vermont Agriculture Station

Pengfei Hou; Wenting Dai; Yanshan Jin; Fengqi Zhao; Jianxin Liu, Hongyun Liu. (2022). Maternal exposure to di-2-ethylhexyl phthalate (DEHP) depresses lactation capacity in mice. Science of The Total Environment 837, 1 September 2022, 155813. doi: 10.1016/j.scitotenv.2022.155813

Caihong Wang, Feng-Qi Zhao, Jianxin Liu, and Hongyun Liu. (2022). The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells. Animal Nutrition 10:12-18. doi: 10.1016/j.aninu.2021.11.00

 

Abstracts

University of Minnesota

Pena Mosca, F., Ruch, M., Florentino, C.C., Mahmoud, M., Godden, S.M., Royster, E., Boyum, B., Rowe, S., Caixeta, L.S. “Negatively controlled trial investigating the effect of dry cow therapy on prevalence of intramammary infections in first lactation dairy cows.” 62nd Annual Meeting of the National Mastitis Council, Atlanta, GA.  January 2023.

Pena Mosca, F.*, Dean, C., Machado, V.S., Fernandes, L., Pinedo, P., Doster, E., Manriquez, D., Heins, B., Sharpe, K., Ray, T., Noyes, N., Caixeta, L.S. “The association between the presence of intramammary infections in the first month of lactation and high somatic cell count during the first six months of lactation in first-lactation organic dairy cows.” 62nd Annual Meeting of the National Mastitis Council, Atlanta, GA. January 2023

Godden, S., E. Royster, E. Leonard, D. Albrecht, J. Timmerman. Pilot Study: Refining a Culture-Guided Selective Dry Cow Therapy Program to Enhance Antimicrobial Stewardship on Dairy Farms. Mastitis Research Workers Conference. Chicago, IL Nov. 2-4, 2022.

Leonard, E., Crooker, B.A., Dow, S., Caixeta, L.S. “Intramammry liposome-TLR agonist (LTC) dose titration: effect on differential somatic cell count.” ADSA Conference, Ottawa, Canada, June 2023.

University of Missouri

Kurban D, Roy JP, DeVries TJ, Adkins PRF, Middleton JR, Keefe GP, France A, and Dufour S.  2023.  Staphylococcal intramammary infections in dairy cows: prevalence at calving in first lactation cows and prevalence, incidence and persistence over the dry period.   American Dairy Science Association.

Kurban D, Roy JP, DeVries TJ, Adkins PRF, Middleton JR, Keefe GP, France A, and Dufour S.  2023.  Staphylococcal intramammary infections in dairy cows: prevalence, incidence and persistence over the dry period.  Canadian Association of Veterinary Epidemiology and Preventive Medicine.  University of Guelph, Ontario.  31 May 23 – 1 Jun 23.

Isensee PK and Adkins PRF. Characterization of antibacterial activity of Staphylococcus chromogenes isolates originating from dairy cattle. MU CVM Research Day. May 5, 2023.

Novo A and Adkins PRF. Intramammary infections in primiparous dairy heifers. MU CVM Research Day. May 5, 2023.

Michigan State University

Kolar, Q.K., K.C. Krogstad, V. Mavangira, T.H. Swarz, I. Yoon, B. J. Bradford and P.L. Ruegg. 2023.  Effects of feeding with Saccharomyces cerevisiae fermentation product on milk oxylipids prior to and during an intramammary S. uberis challenge.  Presented at Ann. Meeting Am. Dairy Sci Assoc., July 2023, Montreal, Canada.

Bacigalupo-Sanguesa, P.L. Ruegg, R. Thomson, B. Norby, A. Contreras and R. Erskine.  2023.  Long Term Impact of Delayed Milk Ejection on Milk Production.  Pp 129-130 in Proceedings of the 62nd Annual M  eeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Bacigalupo-Sanguesa, P, P.L. Ruegg, R. Thomson, B. Norby, A. Contreras and R. Erskine.  2023.  Identificaion of delayed milk ejection episodes using milk flow recordings. Pp 141-142 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Goncalves, J., R. Mani, Q. Kolar, S. Sreevatsan, and P.L. Ruegg.  2023.  Distribution of minimum inhibitory concentrations of selected antibiotics for mecA Positive and negative Non-aureus Staphylococci that exhibit phenotypic resistance to methicillin.   Pp 169-170 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Kolar, Q.K, J. L. Goncalves, S.M. Godden,  R. J. Erskine, and P.L. Ruegg.  Comparison of in-vitro resistance of Non-aureus Staphylococci, Enterococci, Lactococci, and other Strep-like organism to approved intramammary antimicrobials.  Pp 161-162 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Rodriguez, Z., V.E. Cabrera, H. Hogeveen, and and P. L. Ruegg.  2023.  Economic simulation to evaluate the cost-effectiveness of subclinical mastitis treatment using nisin during early lactdation – preliminary results.  Pp 133-134 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Pinzon-Sanchez, C., P. Bacigalupo, M. Buza, P. Durst, M. Mangual, V. Malacco, and P.L. Ruegg.  2023.  Benchmarking Antibiotic Usage:   A Practical Online Tool. Pp 167-168 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

South Dakota State University

Kolar, Q. K., K. C. Krogstad, V. Mavangira, T. H. Swartz, I. Yoon, B. J. Bradford, and P. L. Ruegg. Effects of supplementation with Saccharomyces cerevisiae fermentation product on milk oxylipids prior to and during an intramammary challenge with S. uberis. American Dairy Science Association. Ottawa, Ontario. June 25-28, 2023.

Swartz, T. H., B. J. Bradford, L. K. Mamedova, and K. A. Estes. Effects of dietary rumen-protected choline supplementation on choline metabolites and inflammatory markers in mammary tissue during an intramammary lipopolysaccharide challenge. American Dairy Science Association. Ottawa, Ontario. June 25-28, 2023.

Swartz, T. H., B. J. Bradford, L. K. Mamedova, and K. A. Estes. Effects of dietary rumen-protected choline supplementation on the plasma metabolome during an intramammary lipopolysaccharide challenge. American Dairy Science Association. Ottawa, Ontario. June 25-28, 2023.

Kolar, Q. K., K. C. Krogstad, T. H. Swartz, I. Yoon, P. Coussens, M. Bernard, V. Mavangira, B. J. Bradford, and P. L. Ruegg. Effect of supplementation with Saccharomyces cerevisiae fermentation product on mammary immune cell populations. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 20-24, 2023.

Rodriguez, Z., Q. Kolar, K. Krogstad, T. H. Swartz, I. Yoon, B. J. Bradford, and P. L. Ruegg. Evaluation of reticuloruminal temperature for prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 20-24, 2023.

 

Conference Proceedings

University of Missouri

Lopez-Benavides M, Haw S, Britt L, Partridge J, Leibowitz J, Middleton J.  2023.  Teat canal measurements around milking using ultrasound.  Proceedings of the 62nd Annual Meeting of the National Mastitis Council.  Atlanta, Georgia.  30 Jan 2023 – 2 Feb 2023.

Middleton JR, Adkins PRF.  2023. The What, Why, When and How of Mastitis Treatment.  Short course #7 presented at 62nd Annual Meeting of the National Mastitis Council.  Atlanta, Georgia.  31 Jan 2023.  Electronic Note Set.

Adkins PRF.  Antimicrobial use for the treatment of bovine mastitis. American College of Veterinary Internal Medicine Annual Forum (Live Virtual Day) June 28, 2023.

Isensee PK and Adkins PRF.  Characterization of antibacterial activity of Staphylococcus chromogenes isolates originating from dairy cattle. Proceedings of the 61st Annual Meeting of the National Mastitis Council, Atlanta, Georgia. Jan 30- Feb 2, 2023.

Michigan State University

Ruegg, P.L.  Clinical mastitis treatment decisions.   2023. In Proc. 6th Am. Assoc. Bov. Pract. Recent Graduate Conf. Knoxville TN, Feb 10-13, 2023

Rodriguez, Z., V.E. Cabrera, H. Hogeveen, and and P. L. Ruegg.  2023.  Economic simulation to evaluate the cost-effectiveness of subclinical mastitis treatment using nisin during early lactdation – preliminary results.  Pp 133-134 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Pinzon-Sanchez, C., P. Bacigalupo, M. Buza, P. Durst, M. Mangual, V. Malacco, and P.L. Ruegg.  2023.  Benchmarking Antibiotic Usage:   A Practical Online Tool. Pp 167-168 in Proceedings of the 62nd Annual Meeting of the NMC, Jan 30-Feb 2, Atlanta GA.

Steinberger, A., Leite de Campos, J., T. Goldberg, P. Ruegg, N. Safdar, J. Sutske, A. Sethi, and G. Suen.  Investigating the role of dairy farm antimicrobial use on the bovine fecal resistome.  Presented at Conf. Research Workers Animal Disease, Chicago IL, Jan 2023

Rodriguez, Z., Q.K. Kolar, K.C. Krogstad,T. H. Swartz, I.Yoon, B. J. Bradford, and P. L. Ruegg.  2023.  Evaluation of reticuloruminal temperature for prediction of clinical mastitis in dairy cows challenged with Streptococcus uberis. Presented at Conf. Research Workers Animal Disease, Chicago IL, Jan 2023

Goncalves, J., Mani, R., Kolar, Q., Sreevatsan, S. and P.L. Ruegg.  2023.  Prevalence of methicillin-resistant and susceptible Staphylococci spp., in bulk tank milk from dairy herds in Michigan. Presented at Conf. Research Workers Animal Disease, Chicago IL, Jan 2023

Utah State University

Wilson DJ, Goodell GM, Dumm R, Kelly T, Bethard M: Sodium percarbonate as a preservative in waste milk fed to dairy calves. Proc 74th Ann Conf European Federation of Animal Science (EAAP): 534, 2023.

Wilson DJ, Goodell GM, Dumm R, Kelly T, Bethard M: A preservative for waste/diverted milk fed to dairy calves.  Mast Res Workers, Ghent, Belgium, 2024

 

Poster Presentations

Rutgers University; New Jersey Agricultural Experiment

Esquilín-Lebrón K., Foley M., Carabetta V., Beavers W., Skaar E., and  Boyd J.M.  YlaN functions in iron homeostasis in Staphylococcus aureus. Society for Advancement of Chicanos/Hispanics and Native Americans in Science Annual Meeting. Virtual 10/2021.

Ficken K. and Boyd J.M.  Investigating Bacteriophages as a Novel Therapeutic Approach against Common Skin-Infecting Bacteria. Aresty research symposium, Rutgers University, New Brunswick, NJ 3/2022.

McGinley C.M. and Boyd J.M.  Investigation into the roles of conserved amino acids for the function of the metal ion homeostasis protein YlaN. Aresty research symposium, Rutgers University, New Brunswick, NJ 3/2022.

Rios-Delgado G., Boyd J.M.  The role of the small RNA tsr25 in Staphylococcus aureus iron homeostasis Rutgers Initiative for Maximizing Student Development (IMSD) symposium, New Brunswick, NJ 5/2022.

Rios-Delgado G., Boyd J.M.  The role of the small RNA tsr25 in Staphylococcus aureus iron homeostasis New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2022.

Román-Rodríguez F. and Boyd J. M.  Examining the stimuli of the SrrAB regulatory system in Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2022.

Rios-Delgado G., Boyd J.M.  The role of the small RNA tsr25 in Staphylococcus aureus iron homeostasis New Jersey American Society of Micrbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2022

Norambuna J. and Boyd J.M.  Cu ion homeostasis in Staphylococcus aureus New York Bacillus Interest Group (NYBIG) annual conference, New York, NY. 6/2022.

Ranainghe, L., Ryan Kaler K., Boyd J.M. Functional Assessment of the Regulon of the Ferric Uptake Regulator (Fur) in Staphylococcus aureus Aresty research symposium, Rutgers University, New Brunswick, NJ 3/2023.

Ranainghe, L., Ryan Kaler K., J.M. Boyd Functional Assessment of the Regulon of the Ferric Uptake Regulator (Fur) in Staphylococcus aureus Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2023.

Rios-Delgado G., Norambuena J., Ryan Kaler K., Briaud P., Mustor E., Shaw L., Parker D., Carrol R., J.M. Boyd. The role of Tsr25 in iron homeostasis in Staphylococcus aureus. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2023.

Roman Rodriguez F. and Boyd J.M. Examining the effects of Pseudomonas aeruginosa secondary metabolites on Staphylococcus aureus transcriptional networks. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2023.

Roman Rodriguez F. J.M. Boyd. Examining the effects of Pseudomonas aeruginosa secondary metabolites on Staphylococcus aureus transcriptional networks. New York Bacillus Interest Group (NYBIG) annual conference, New York, NY. 6/2023.

Rios-Delgado G., Norambuena J., Ryan Kaler K., Briaud P., Mustor E., Shaw L., Parker D., Carrol R., J.M. Boyd. Genetic regulation of iron homeostasis in Staphylococcus aureus. Gordon Research Conference on Staphylococcal Diseases. Waterville Valley, NH 7/2023.

Paola Garay Maris, Gustavo Rios, J.M. Boyd. Staphylococcus aureus iron usage: characterization of a novel antibiotic target. RISE at Rutgers annual conference, New Brunswick, NJ. 8/2023

Paola Garay Maris, Gustavo Rios, J.M. Boyd. Staphylococcus aureus iron usage: characterization of a novel antibiotic target. Intra-College Experience in Creative Research in Experimental and Applied Microbiomics, Rutgers University, New Brunswick, NJ. 8/2023.

University of Missouri

Adkins PRF and Novo A. Evaluation of intramammary infection status in dairy heifers using two different sample collection techniques. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 20-24, 2023.

Outreach Presentations

Rutgers University; New Jersey Agricultural Experiment

Copper ion homeostasis in Staphylococcus aureus. Rutgers University Dept. of Biochemistry and Microbiology. Virtual 10/2021.

Studying Staphylococcus aureus physiology to decrease disease burden. Rutgers University Molecular Biology and Biochemistry club.  Virtual 3/2022.

Metal ion homeostasis in Staphylococcus aureus. Chemistry Department, Fordham University. 10/2022.

Genetic regulation of iron homeostasis in Staphylococcus aureus. International conference on iron-sulfur proteins—Biogenesis, Regulation, and Function. Saint Tropez, FR 8/2022.

Mechanisms of copper ion intoxication and detoxification in Staphylococcus aureus. Louisiana State University, Dept of Pathobiological Sciences 11/2022.

Metal ion homeostasis in Staphylococcus aureus. Rutgers School of Dental Medicine. Newark, NJ 2/2023.

Copper ions and the Staphylococcus aureus host interface. International Conference of Staphylococcus aureus and Staphylococcal Infections. Virtual 2/2023.

Copper ions and the Staphylococcus aureus host interface. International Conference of Staphylococcus aureus and Staphylococcal Infections. Southern Illinois Medical School, Springfield, IL 2/2023.

Iron homeostasis in Staphylococcus aureus. University of Illinois, Urbana, IL 9/2023.

 

05/14/2025

 

Peer-Reviewed Literature

Cornell University

1. Kolar, Q. K., Godden, S. M., Erskine, R. J., & Ruegg, P. L. (2024). Confirmed bacteriological diagnosis and cure of non-severe Gram-positive clinical mastitis cases enrolled in a randomized clinical trial based on results of on-farm culture. JDS Communications.


2. Kolar, Q. K., Goncalves, J. L., Erskine, R. J., & Ruegg, P. L. (2024). Comparison of minimum inhibitory concentrations of selected antimicrobials for non-aureus Staphylococci, Enterococci, Lactococci, and Streptococci isolated from milk samples of cows with clinical mastitis. Antibiotics, 13(1), 91.


3. Wieland M, Skarbye AP. A retrospective cohort study investigating the association of individual quarter dry-off with udder health, milk production, and culling risk. J Dairy Sci, 2024, 107:4941-4960

University of Minnesota

4. Dean, C.J., Deng, Y., Wehri, T.C., Pena Mosca, F., Ray, T., Crooker, B.A., Godden, S.M., Caixeta, L.S., Noyes, N.R. The impact of kit, environmental and sampling contamination on the observed microbiome of bovine milk. mSystems 9:e01158-23 2024. https://doi.org/10.1128/msystems.01158-23


5. Florentino, C.C. &, F. Peña-Mosca, M. Ruch, E. Shepley, M. Barbosa, D. Moreira, M. Mahmoud, L. Tikosky, W. Knauer, G. Cramer, S. Godden, L. Caixeta. 2024. Randomized clinical trial evaluating the effects of administering acidogenic boluses at dry-off on udder health, milk yield, and herd removal. J. Dairy Sci. 107:3899–3915 https://doi.org/10.3168/jds.2023-23757


6. Dean, C. &, F. Peña-Mosca, T. Ray, T. Wehri, K. Sharpe, A. Antunes Jr, E. Doster, L. Fernandes, V. Calles, C. Bauman, S. Godden, B. Heins, P. Pinedo, V. Machado, L. Caixeta, and N. Noyes. 2024. Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infection risk in primiparous cows under organic directives. AppEnvirMicro 90:1-23 https://doi.org/10.1128/aem.02234-23


7. Peña-Mosca, F. &, S. Godden, E. Royster, B.A. Crooker, and J. Timmerman. 2024. Methods of processing recycled manure solids bedding on Midwest dairy farms II: Relationships between bedding characteristics and bedding bacteria count. Bov Pract. 58:8-18


8. Brink, A.A. &, W.J. Weber, J.D. Lippolis, J.B. Cole, A.K. Rendahl, L. Caixeta, S.M. Godden, A.J. Seykora, and B.A. Crooker. 2023. Effect of Holstein genotype on ex-vivo interleukin-1β response to lipopolysaccharide (LPS), lipoteichoic acid (LTA) and heat-killed Gram-negative and Gram-positive bacteria. Vet Imm & Immunopathology. 258:110573 https://doi.org/10.1016/j.vetimm.2023.110573


9. de Jong, E. L. Creytens, S. De Vliegher, K. McCubbin, M. Baptiste, A.A. Leung, D. Speksnijder, S. Dufour, J. Middleton, P.L. Ruegg, T.J.G.M. Lam, D.F. Kelton, S. McDougall, S.M. Godden, A. Lago, P.J. Rajala-Schultz, K. Orsel, V. Krömker, J.P. Kastelic, and H.W. Barkema. 2023. Selective treatment of non-severe clinical mastitis does not negatively affect cure, somatic cell count, milk yield, recurrence and culling: a systematic review and meta-analysis. J Dairy Sci 106:1267-1286 https://doi.org/10.3168/jds.2022-22271

University of Missouri

10. Haw SR, Adkins PRF, Bernier Gosselin V, Poock SE, Middleton JR. 2024. Intramammary infections in lactating Jersey cows: prevalence of microbial organisms and association with milk somatic cell count and persistence of infection. J Dairy Sci.107 (5): 3157-3167. DOI: 10.3168/jds.2023-23848

The Ohio State University

11. Gammariello, C. S., J. Hanson, A. E. Relling, M. X. S. Oliveira, A. Sipka, K. M. Enger, and B. D. Enger. 2024. Localized mammary gland changes in milk composition and venous blood metabolite concentrations result from sterile subclinical mastitis. J. Dairy Sci. 107:6148-6160. https://doi.org/10.3168/jds.2023-24044.

Rutgers University; New Jersey Agricultural Experiment

12. Muneeswaran Z.P., Teoman B., Wang Y., Chaudhry H., Brinzari T.V., Verma G., Ranasinghe L., Ryan Kaler K.M., Huang K., He, X., Thomas B.L., Xu S., Cheng C.Y., Boyd J.M., Chen D., Hao Z., Ma S., Asefa T., Pan L., Dubovoy V., Novel anionic surfactant-modified chlorhexidine and its potent antimicrobial properties. 12/2023 Dalton Transactions PMID: 38224288.

South Dakota State University

13. Swartz, T. H.*, B. J. Bradford, L. K. Mamedova, and K. A. Estes. 2024. Effects of dietary rumen-protected choline supplementation to periparturient dairy cattle on inflammation and metabolism in mammary and liver tissue during an intramammary lipopolysaccharide challenge. J. Dairy Sci. 107:1211-1227. https://doi.org/10.3168/jds.2023-23752


14. Swartz, T. H., B. J. Bradford, L. K. Mamedova, and K. A. Estes. 2023. Effects of dietary rumen-protected choline supplementation to periparturient dairy cattle on inflammation, metabolism, and performance during an intramammary lipopolysaccharide challenge. J. Dairy Sci. 106:8561-8582. https://doi.org/10.3168/jds.2023-23259

University of Vermont Agriculture Station

15. Chrissy Lalonde, Jana Kraft, and Feng-Qi Zhao. (2024). Review: Molecular aspects of milk lipid synthesis and secretion in mammary epithelial cells. Animal Nutriomics, in press. doi: 10.1017/anr.2024.19


16. Miyuki Takashima, Masahiro Kurita, Haruhi Terai, Feng-Qi Zhao, and Jun-ichiro Suzuki. (2024). S-allylmercaptocysteine inhibits lipopolysaccharide-induced inflammation via altering phosphorylation of spliceosomal components in mammary epithelial cell. Scientific Reports, in press


17. Yongxin Li, Ning Han, Pengfei Hou; Feng-Qi Zhao, Hongyun Liu. (2024). Roles of MAPK and Nrf2 signaling pathways in quercetin alleviating redox imbalance induced by hydrogen peroxide in mammary epithelial cells. Animal Nutriomics 1, e1, 1–10. doi: 10.1017/anr.2024.2


18. Jeffrey, C. E.c, Andrews, T., Godden, S. M., Neher, D. A., Barlow, J. W.a (2024). Relationship between facility type and bulk tank milk bacteriology, udder health, udder hygiene, and milk production on Vermont organic dairy farms. Journal of Dairy Science, 107(10), 8534–8553. https://doi.org/10.3168/jds.2023-24576 Product of USDA-NIFA-OREI award number 2018-51300-28561 and USDA-NIFA-Predoctoral Fellowship award number 2022-67011-3656


19. Chakrawarti, A.c, Eckstrom, K., Laaguiby, P., Barlow, J.W.a 2024. Hybrid Illumina-Nanopore assembly improves identification of multilocus sequence types and antimicrobial resistance genes of Staphylococcus aureus isolated from Vermont dairy farms: comparison to Illumina-only and R9.4.1 nanopore-only assemblies. Access Microbiology https://doi.org/10.1099/acmi.0.000766.v3 Product of USDA-Hatch Vermont Experiment Station project (multi-state project NE1748, award number VT-H02413MS) and Vermont Animal Health Formula Funds (awards VT-AH02703 and VT-2023-AH01).


20. Chakrawarti, A.c, Casey, C.L., Burke, A.d, Mugabi, R.c, Ochoa, A., Barlow, J.W.a 2024. An observational study demonstrates human-adapted Staphylococcus aureus strains have a higher frequency of antibiotic resistance compared to cattle-adapted strains isolated from dairy farms making farmstead cheese. BMC Veterinary Research 20:75 DOI: 10.1186/s12917-024-03910-6 Product of USDA-Hatch Vermont Experiment Station project (multi-state project NE1748, award number VT-H02413MS).

Abstracts

Cornell University

1. Somula H, Singh A, Spellman ME, Wieland M. The effect of individual quarter dry-off on milk production, occurrence of mastitis, and culling risk in Holstein dairy cows. 32^nd World Buiatrics Congress, Cancun, Mexico, 2024

University of Minnesota

2. C.C., W. Knauer*, L. Tikofsky, S.M. Godden, L. Caixeta, F. Peña-Mosca, D. Nydam. 2024. Randomized clinical trial evaluating the effects of the administration of acidogenic boluses on the day prior to dry-off on dairy cow welfare. AABP 27^th Annu Conference. Columbus, OH. Sept 12-14, 2024

University of Missouri

3. Isensee PK, Adesola RO, and Adkins PRF. Characterization of antibacterial activity and strain variability of Staphylococcus chromogenes isolates originating from dairy cattle. MU CVM Research Day. May 3, 2024.


4. Novo A and Adkins PRF. Intramammary infections in primigravid and nulligravid dairy heifers. MU CVM Research Day. May 3, 2024.


5. Novo A and Adkins PRF. Evaluation of intramammary infection status in dairy heifers. Proceedings of the 63rd Annual Meeting of the National Mastitis Council, Dallas, Texas, Jan 29 – Feb 1, 2024.


6. Isensee PK, Adesola RO, and Adkins PRF. Characterization of antibacterial activity and strain variability among Staphylococcus chromogenes isolates originating from dairy cattle. Proceedings of the 63rd Annual Meeting of the National Mastitis Council, Dallas, Texas, Jan 29 – Feb 1, 2024.


7. Adkins PRF, Adesola RO, and Barlow JW. Using MALDI-TOF mass spectrometry to determine Staphylococcus chromogenes strain types. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 21-24, 2024.

The Ohio State University

8. X. S. Oliveira, K. M. Enger, and B. D. Enger. 2024. Impact of Staphylococcus aureus intramammary infection on mammary cell proliferation in pregnant heifers. J. Dairy Sci. 107: (Suppl. 1):106-107.


9. Enger, K. M., S. Park, A. E. Relling and B. D. Enger. 2024. Mineral composition of whole milk from mammary glands repeatedly challenged with killed Staphylococcus aureus. J. Dairy Sci. 107: (Suppl. 1):312.


10. Enger, B. D., G. Hastings, C. S. Gammariello, M. X. S. Oliveria, and K. M. Enger. 2024 Effects of repeated infusions of killed Staphylococcus aureus on milk yield and composition. J. Dairy Sci. 107:(Suppl. 1):106.


11. Enger, B.D., M. X. S. Oliveria, P. H. Baker, C. S. Gammariello, K. M. Enger. M. A. McGuire, and J. Williams. 2024. Intramammary infections in primigravid heifer mammary glands during progressing stages of gestation. Page 324 in Conf. of Res. Workers in Animal Dis. Proc.


12. Oliveira, M.X.S., C.S. Gammariello, P.H. Baker, K.M. Enger, D.D., Clevenger, S.K. Jacobi, N.M. Bello, and B.D. Enger. 2024. Impact of intramammary infections to the developing mammary tissue of dairy heifers at different stages of pregnancy. Pages 95-96 in Natl. Mastitis Counc. Annu. Mtg. Proc., Dallas, TX, Natl. Mastitis Council Inc., New Prague, MN.

Rutgers University; New Jersey Agricultural Experiment

13. Gustavo Rios, Boyd J.M. Assessing the role of the Fur-regulated sRNA Tsr25 during Staphylococcus aureus response to iron scarcity. Initiative for maximizing student development, Rutgers University, Piscataway, NJ 10/2023.


14. Tyagi N., and Boyd J.M. Determining how itaconic acid modulates Staphylococcus aureus physiology. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 4/2024.

South Dakota State University

15. Celemin Sarmiento, A., B. J. Bradford, L. K. Mamedova, and T. H. Swartz. The effects of dietary rumen-protected choline on mammary DNA methylation patterns. American Dairy Science Association. West Palm Beach, FL. June 16-19, 2024.


16. Bulnes, M. A. Celemin Sarmiento, M. R. A. Redoy, J. Lefler, C. Marotz, M. Embree, T. H. Swartz, and M. E. Uddin. Effects of dietary rumen-derived microbial product supplementation on lactation performance and enteric methane emission in lactating dairy cows. American Dairy Science Association. West Palm Beach, FL. June 16-19, 2024.


17. Bulnes, M. A. Celemin Sarmiento, M. R. A. Redoy, J. Lefler, C. Marotz, M. Embree, T. H. Swartz, and M. E. Uddin. Effects of dietary rumen-derived microbial product supplementation on rumen fermentation in lactating dairy cows. American Dairy Science Association. West Palm Beach, FL. June 16-19, 2024.

University of Vermont Agriculture Station

18. Lalonde C, Kraft J, Zhao F-Q (2024) Supplementing cell culture media with essential fatty acids alters the response of MAC-T cells to inflammatory mediators in triacylglyceride synthesis. ADSA Annual Meeting 2024.


19. Barlow, J., Adrian, R., Chakrawarti, A., Jeffrey, C.E., Mugabi, R, Ochoa, A., Machado de Sant’Anna, F., Munk, P. 2024. Case Study: Genetic characterization of methicillin and lincosamide resistant Mammaliicoccus fleurettii isolated from cattle and humans on a Vermont organic dairy farm. National Mastitis Council Regional meeting, Ghent, Belgium, August 11-13.


20. Barlow, J., Chakrawarti, A., Jeffrey, C.E., Machado de Sant’Anna, F., Munk, P. 2024. Use of Flankophile pipeline to infer antimicrobial resistance gene synteny among mastitis pathogens: 3 examples. Mastitis Research Workers annual meeting, Ghent, Belgium, August 8-9.


21. Jeffrey, C.E., Adkins, P.R.F, Barlow, J.W. 2024. Prevalence and Effect on Somatic Cell Count of Non-aureus Staphylococci and Mammaliicocci Intramammary Infections on Organic Dairies in Vermont. Proceedings of National Mastitis Council, 63thrd Annual Meeting, Dallas, TX January 29 – February 1.

 

Conference Proceedings

University of Minnesota

1. Bedding selection and management: An important determinant in producing quality milk. Upper Midwest Dairy Industry Assoc. (UMDIA). St. Cloud, MN. 09/26/2024


2. Preconference Seminar: Understanding the impacts of switching to various forms of solids bedding on herd health, operations, and the environment. 57^th Annu Conf Am Assoc. Bov Pract. Sept. 11, 2024. Columbus, OH. Two presentations:
   
   
   
   1. Sand bedding: Benefits, monitoring and management
   
   
   2. Recycled manure solids bedding: Pros/Cons, monitoring and managagement
   
   
   
   


3. Methods of processing recycled manure solids bedding on Midwest dairy farms II: Relationships between bedding characteristics and bedding bacteria count. AABP. 01/29/2024


4. WVMA. Management of recycled manure solids bedding. 11/3/23

University of Missouri

5. Adkins PRF. Tools and tips for diagnosing intramammary infections. NMC Regional Meeting. Ghent, Belgium. Aug 12-14, 2024.


6. Middleton JR and Adkins PRF. Mastitis diagnosis: what’s the goal? Proceedings of the 63rd Annual Meeting of the National Mastitis Council, Dallas, Texas, Jan 29 – Feb 1, 2024.

South Dakota State University

7. Swartz, T. H. 2024. Nutrition, Metabolism, and Mastitis. National Mastitis Council Regional Meeting Proceedings.

University of Vermont Agriculture Station

8. Barlow, J. 2024. “Antibiotic resistance genes, cows, and fermented food: Antimicrobial resistance and resistomes on dairy farms in Vermont.” International Conference on the Science of Artisan Cheese. August, Somerset, England.

 

Poster Presentations

Cornell University

1. Somula H, Singh A, Spellman ME, Wieland M. The effect of individual quarter dry-off on milk production, occurrence of mastitis, and culling risk in Holstein dairy cows. 32nd World Buiatrics Congress, Cancun, Mexico, May 20th – 24th, 2024

Rutgers University; New Jersey Agricultural Experiment

2. Paola Garay Maris, Gustavo Rios, Boyd J.M.  Staphylococcus aureus iron usage: characterization of a novel antibiotic target. National meeting for the Society for the Advancement of Chicanos/Hispanics and Native Americans in Science. Portland OR. 10/2023.


3. Norambuena J. and Boyd J.M.  Unravelling copper toxicity mechanisms in Staphylococcus aureus”. Reunión anual de la Sociedad de Bioquímica y Biología Molecular de Chile, Coquimbo, Chile. 10/2023.


4. Leon G. Leanse, Boyd J.M. et al., Unravelling the antimicrobial mechanisms of blue light. Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases, San Francisco, CA. 2/2024.


5. Tyagi N., and Boyd J.M. Determining how itaconic acid modulates Staphylococcus aureus physiology. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 4/2024.


6. Tyagi N., Wong T., and Boyd J.M. Determining how itaconic acid modulates Staphylococcus aureus physiology. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 4/2024.


7. Naidu N., Chen S., Rios-Delgado G., Boyd J.M. Exploring Fur and IsrR genetic regulation in Staphylococcus epidermidis. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 4/2024.


8. Bovermann H., Boyd J.M. Impact of Sulfur Metabolism on Copper and Silver homeostasis in Staphylococcus aureus. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2024.


9. Naidu N., Chen S., Rios-Delgado G., Boyd J.M. Exploring Fur and IsrR genetic regulation in Staphylococcus epidermidis. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2024.


10. Tyagi N., Wong T., and Boyd J.M. Determining how itaconic acid modulates Staphylococcus aureus physiology. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2024.


11. Vincent Zheng, Rios-Delgado G., and Boyd J.M. The Relationship Between Iron Homeostasis and Biofilm Formation in S. epidermidis. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 4/2024.


12. Rios-Delgado G. and Boyd J.M. et al., Fur-regulated small RNA IsrR represses TCA cycle during iron limitation in Staphylococcus aureus. International meeting on Fe-S proteins: Function, Biogenesis, and Regulation. Wake Forest University, Winston-Salem, NC 6/2024.


13. Rios-Delgado G., Boyd J.M., et al., Fur-regulated small RNA IsrR represses TCA cycle during iron limitation in Staphylococcus aureus. International meeting on Fe-S proteins: Function, Biogenesis, and Regulation. Wake Forest University, Winston-Salem, NC 6/2024.

01/02/2026

Publications

Peer-Reviewed Literature

Works representing collaborations between multiple member institutions

Minnesota and Cornell

1. Peña-Mosca, F., Nydam, D.V., Caixeta, L.S., Couto Serrenho, R., Thomas, M.; Saila, S., Bork, O., Stangaferro. M. “Evaluation of an automated on-farm device for the identification of bacteria associated with clinical mastitis in United States.” Bovine Practitioner. 2025. 59(2), 26-31. https://doi.org/10.21423/bpj20259258


2. Rowe, S.M., E. Zhang, S.M. Godden, A.K. Vasquez, and D.V. Nydam. 2025. Comparison of a machine learning model with a conventional rule-based selective dry cow therapy algorithm for detection of intramammary infections. J Dairy Sci 108:760-772 https://doi.org/10.3168/jds.2024-25418


3. Rowe, S., S. Godden, A. Vasquez, and D.V. Nydam. 2025. A Practitioner’s Guide to Selective Dry Cow Therapy. Veterinary Clinics of North America – Food Animal Practice. Mastitis. Elsevier Press, Inc. 41:209-222 July 2025 https://www.vetfood.theclinics.com/article/S0749-0720(25)00017-9/fulltext

Cornell, Michigan and South Dakota State

4. Kolar, Q. K., K. C. Krogstad, T. H. Swartz, L. K. Mamedova, V. Mavangira, I. Yoon, B. J. Bradford, and P. L. Ruegg. "Effect of dietary supplementation with Saccharomyces cerevisiae fermentation product on clinical outcomes in dairy cows during challenge with Streptococcus uberis." Journal of Dairy Science (2025).

Michigan and Cornell

5. Kolar, Q., S. Godden, R. Erskine, and P.L. Ruegg.   SHORT COMMUNICATION:  Confirmed bacteriological diagnosis and clearance of non-severe Gram-positive clinical mastitis cases enrolled in a randomized clinical trial using an on-farm culture program.  J Dairy Sci Comm.  5:628-633   https://doi.org/10.3168/jdsc.2024-0560


6. Kolar, Q.K., J. L. Goncalves, R. J. Erskine, and P.L. Ruegg.   Comparison of minimum inhibitory concentrations of selected antimicrobials for non-aureus Staphylococci, Enterococci, Lactococci, and Streptococci isolated from milk samples of cows with clinical mastitis. Antibiotics 13,91.  https://doi.org/10.3390/antibiotics13010091 

Michigan and Missouri

7. Ruegg, P.L. and P.R.F. Adkins.   Mastitis in Dairy Cows.  in Production Diseases of Farm Animals:  Pathophysiology, Prophylaxis and Health Management.  J. J. Gross editor.   Springer Nature.  ISBN: 978-3-031-51787-7..  pp 339- 365 https://link.springer.com/chapter/10.1007/978-3-031-51788-4_15#DOI 

Missouri and Vermont

8. Jeffrey CE, Adkins PRF, Dufour S, Barlow JW.   Staphylococci and mammaliicocci: which species are important for udder health on organic dairy farms. J Dairy Sci. 108 (2): 1807-1823 https://doi.org/10.3168/jds.2024-25320

Works from member institutions

Cornell University

9. Wieland M, Paulrud C, Kakoulis K, Spellman M, Somula H, Sing A. An observational study investigating the association of teat condition with clinical mastitis risk. J Dairy Sci, 2025,108(12):13654-13666


10. Singh A, Somula H, Wieland M. A retrospective cohort study investigating the association of postcalving intramammary infection and milk yield, somatic cell count, clinical mastitis, and culling risk in first-lactation dairy cows. J Dairy Sci, 2025, 108(4):4234-4247


11. Spellman M, Geary CM, Somula H, Singh A, Wieland M. The association between teat shape and clinical mastitis. J Dairy Sci, 2025, 108(1):773-780

Long Island University

12. Mahmmod Y, Smit N, Klapproth H, Chaffer M, Zvonar M. Diagnostic performance of a novel molecular microarray assay for detection of bovine intramammary infections with Mycoplasma species using a gold standard approach. J Dairy Res. 2025 Nov 17:1-5. doi: 10.1017/S0022029925101672.


13. Tartor YH, Enany ME, Ismail NI, El-Demerdash AS, Eidaroos NH, Algendy RM, Mahmmod Y, Elsohaby I. Vancomycin-resistant Staphylococcus aureus endangers Egyptian dairy herds. Sci Rep. 2024 Dec 23;14(1):30606. doi: 10.1038/s41598-024-81516-6.

Michigan State University

14. Ruegg, P.L. 2025. Making economically efficient treatment decisions for clinical mastitis. Veterinary Clinics of North America, Food Animal Practice.  Mastitis in Ruminants.  2025 Apr 4:S0749-0720(25)00015-5.https://doi.org/10.1016/j.cvfa.2025.02.003 


15. Hoang,T.A.P. C.Robison, and P.L. Ruegg. Evaluation of in-vitro inhibitory activity of extracts of garlic, ginger and onion against Escherichia coli and Staphylococcus aureus isolated from milk of dairy cows.  Veterinary Sciences, 12,947 https://doi.org/10.3390/vetsci12100947


16. Steinberger, A.J., J.L. De Campos, A.E. Kates, T.L. Goldberg, P.L. Ruegg, N. Safdar, A.K. Sethi, J.M. Shutske, G. Suen. 2025. Assessing the impacts of dairy farm antimicrobial use on the bovine fecal microbiome. Animals 15:1735 https://doi.org/10.3390/ani15121735 


17. De Souza Ferreira, L., T. Showemimo, L.B. Juliano, Z. Rodriguez, and P.L. Ruegg. Use of MALDI-TOF to identify cryopreserved mastitis pathogens collected from 2003 – 2011 that were originally identified using conventional microbiological methods.  J Dairy Sci Comm.  6:422-426. https://doi.org/10.3168/jdsc.2024-0711 


18. Wilson, D.J., E.M. Parker, R. Portillo-Gonzalez, P.L. Ruegg and G. G. Habing. A focus group study exploring necessary competencies and contextual factors for effective antimicrobial stewardship on dairy farms.  J Dairy Science, 108:5182-5192.  https://doi.org/10.3168/jds.2024-25302 


19. Rodriguez, Z.; A. O’Connor; B.J. Bradford; C Picasso-Risso. 2025. Characterization and health, productivity, and economic effects of highly pathogenic avian influenza H5N1 outbreak in dairy cattle. Journal of Dairy Science 108:6349–6358. doi:10.3168/jds.2025-2637


20. Chao, O., Shutske, E. Gonzalez, D. Veeser, E. Diaz Vallejo, A. Sethi, T. Goldberg, A. Kates, M.J. Knobloch, J. Leite de Campos, P. Ruegg, N. Safdar,  A. Steinberger, G. Suen, and A. Young.  2025.  Development and Evaluation of an Intervention to Reduce Occupational Health Risk from Antibiotic Resistant Pathogens Among Dairy Farm Workers.   J Agricultural Safety and Health,.  31:31-45.  https://doi.org/10.13031/jash.16158 


21. Rodriguez, Z., M. Lopez-Benavides, M. B. Gentilini, and P. L. Ruegg. Impact of training Dairy personnel on milking routine compliance, udder health and milk quality.   J Dairy Science 108:1615-1624.  https://doi.org/10.3168/jds.2024-25609   


22. Leite de Campos, J., A. Kates, A. Steinberger, A. Sethi, G. Suen, J. Shutske, N. Safdar, T. Goldberg, and P.L. Ruegg. SHORT COMMUNICATION: Variation in Partial Direct Costs of dry cow therapy on 37 large dairy herds.  J. Dairy Sci Comm.  5:639-643.     https://doi.org/10.3168/jdsc.2024-0568 


23. Vasco, K., Z.A. Hansen, A. L. Schilmiller, B.Bowcutt, S. L. Carbonell, P.L. Ruegg, Robert A. Quinn, L. Zhang, and S. Manning. 2024. Untargeted metabolomics and metagenomics reveal signatures for intramammary ceftiofur treatment and lactation stage in the cattle hindgut Frontiers Molecular Biosciences, 2024 May 21:11:1364637. https://doi.org/10.3389/fmolb.2024.1364637


24. Rodriguez, Z., V. E. Cabrera, H. Hogeveen, and P.L. Ruegg. Economic impact of treatment of subclinical mastitis in early lactation using intramammary nisin.  J Dairy Sci. 107:4634-4645 https://doi.org/10.3168/jds.2023-24311  

University of Minnesota

25. Peña-Mosca, F., M. Ruch, M. Mahmoud, S. Godden, E. Royster, B. Boyum, S. Rowe, and L. Caixeta. Negatively controlled trial investigating the effect of dry cow therapy in first-lactation dairy cows on the prevalence of intramammary infections in the subsequent lactation. JDSci Accepted 11/7/2025


26. Peña-Mosca, F., S. Godden, E. Royster, D. Albrecht, S. Wells, B. Crooker, and N. Aulik. 2025. Relationships between method of processing and presence of mastitis and non-mastitis pathogens in ready-to-use recycled manure solids bedding on Midwest dairy farms. JDSci Communications. In press. https:// doi.org/10.3168/jdsc.2025-0754


27. Dean, C.J., Deng, Y., Wehri, T.C., Pena Mosca, F., Ray, T., Crooker, B.A., Godden, S.M., Caixeta, L.S., Noyes, N.R. The impact of kit, environmental and sampling contamination on the observed microbiome of bovine milk. mSystems 9:e01158-23 2024. https://doi.org/10.1128/msystems.01158-23


28. Florentino, C.C. &, F. Peña-Mosca, M. Ruch, E. Shepley, M. Barbosa, D. Moreira, M. Mahmoud, L. Tikosky, W. Knauer, G. Cramer, S. Godden, L. Caixeta. 2024. Randomized clinical trial evaluating the effects of administering acidogenic boluses at dry-off on udder health, milk yield, and herd removal. J. Dairy Sci. 107:3899–3915 https://doi.org/10.3168/jds.2023-23757


29. Dean, C. &, F. Peña-Mosca, T. Ray, T. Wehri, K. Sharpe, A. Antunes Jr, E. Doster, L. Fernandes, V. Calles, C. Bauman, S. Godden, B. Heins, P. Pinedo, V. Machado, L. Caixeta, and N. Noyes. 2024. Exploring associations between the teat apex metagenome and Staphylococcus aureus intramammary infection risk in primiparous cows under organic directives. AppEnvirMicro 90:1-23 https://doi.org/10.1128/aem.02234-23


30. Peña-Mosca, F. &, S. Godden, E. Royster, B.A. Crooker, and J. Timmerman. 2024. Methods of processing recycled manure solids bedding on Midwest dairy farms II: Relationships between bedding characteristics and bedding bacteria count. Bov Pract. 58:8-18


31. Peña-Mosca, F., Gaire, T.N., Dean, C. J., Frem, P., Manriquez, D., Pablo, P., Noyes, N.R., Caixeta, L.S. “Exploring the phylogenetic diversity and antimicrobial activity of non-aureus staphylococci and mammaliicocci isolated from teat apices of organic dairy cows.” Frontiers in Microbiology. Volume 16 – 2025. https://doi.org/10.3389/fmicb.2025.1614488


32. Dean, C.J., Peña-Mosca, F., Wehri, T.J., Ray, T., Sharpe, K., Antunes Jr., A.M., Doster, E., Fernandes, L., Calles, V.F., Bauman, C., Heins, B., Pinedo, P.J., Machado, V.S., Caixeta, L.S., Noyes, N.R. “The teat skin microbiota of organic primiparous dairy cows is dynamic during the transition period” Preventive Veterinary Medicine. https://doi.org/10.1016/j.prevetmed.2024.106402


33. Godden, S., and E. Royster. 2025. Bedding Management with a View to Promoting Udder Health. Chapter in Veterinary Clinics of North America – Food Animal Practice. Mastitis. Elsevier Press, Inc. Vet Clin Food Anim - (2025). https://doi.org/10.1016/j.cvfa.2025.02.007

University of Missouri

34. Novo AI, Middleton JR, Poock SE, Adkins PRF. Evaluation of intramammary infection status in dairy heifers using cisternal and teat end sampling techniques.  J Dairy Sci.  108 (8): 8819-8830.


35. Kurban D, Cia Z, Roy JP, DeVries TJ, Ronholm J, Adkins PRF, Middleton JR, Keefe GP, France A, and Dufour S. Dynamics and impacts of staphylococcal intramammary infection during dairy cows’ non-lactating periods.  J Dairy Sci.  108 (10): 11317-11338.

The Ohio State University

36. Oliveira, M. X. S., C. S. Gammariello, P. H. Baker, S. K. Jacobi and B. D. Enger. 2025. Impact of intramammary infections on mammary gland development in pregnant dairy heifers during late gestation. J. Dairy Sci. 108:12650-12662. doi.org/10.3168/jds.2025-26734.


37. Baker, P. H., L. M. Glendenning, M. X. S. Oliveira, B. A. Cobb, B. D. Enger and S. N. Langel. 2025. Pregnant dairy heifers express Influenza A virus receptors in the mammary gland. J Dairy Sci. In Press.


38. Enger, B. D., G. Hastings, C. S. Gammariello, M. X. S. Oliveira, and K. M. Enger. 2025. Repeated infusion of killed Staphylococcus aureus has increasingly negative effects on milk yield and composition. J. Dairy Sci. Comm. 6:444-447. doi.org/10.3168/jdsc.2024-0718.

Rutgers University; New Jersey Agricultural Experiment

39. Boyd J.M., Esquilín-Lebrón K., Campbell C.J., Ryan Kaler K., Norambuena J., Foley M.E., Stephens T.G., Rios G., Mereddy G., Zheng V., Bovermann H., Kim J., Yang J.H., Greco T.M., Cristea I.M., Carabetta V.J., Beavers W.N., Bhattacharya D., Skaar E.P., Parker D., Carroll R.K., Stemmler T.L., Fpa (YlaN) is an iron(II) binding protein that functions to alleviate Fur-mediated repression of gene expression in Staphylococcus aureus. 10/2024 mBio PMID: 39440976


40. Pederick J.L. Vandborg B.C., George A., Bovermann H., Boyd J.M., Freundlich J.S., Bruning J.B. Identification of cysteine metabolism regulator (CymR)-derived pentapeptides as nanomolar inhibitors of Staphylococcus aureus O-acetyl-ʟ-serine sulfhydrylase (CysK). 11/2024 ACS Infectious Disease PMID: 39705018


41. Sabo E., Nelson C., Tyagi N., Stark V., Aasman K., Morrison C., Boyd, J.M.*, Holz R.C.*, Practical Spectrophotometric Assay for the Cysteine Desulfurase SufS from Staphylococcus aureus, a Potential Antibiotic Target. 1/2025 Antibiotics (Basel). PMID: 40001373


42. Rios-Delgado G., McReynolds A.K.G., Pagella E.A., Norambuena J., Briaud P., Zheng V., Munneke M.J., Kim J., Racine H., Carroll R., Zelzion E., Skaar E., Bose J.L., Parker D., Lalaouna D., Boyd J.M. The Staphylococcus aureus non-coding RNA IsrR regulates TCA cycle activity and virulence. 2/2025 Nucleic Acids Research PMID: 39704109


43. Boyd J.M., Price E.E., Roman Rodriguez F., Burchat N., Norambuena J., DuMont A.L., Torres V.J., Sampath H., Treatment of Staphylococcus aureus with environmentally relevant concentrations of triclosan activates SaeRS-dependent virulence factor expression. 6/2025 Antimicrobial Agents and Chemotherapy. PMID: 40531055


44. Chen Y.T., Liu Z., Fucich D., Giulieri S.G., Liu Z., Wadhwa R., Rios G., Henschel H., Tyagi N., Olivier F.A.B., Monk I.R., Shaw S.S., Sridhar S.H., Drikic M., Bianco C., Lohia G., Beg A.Z., Planet P., Lewis I.A., Sebra R., Traven A., Hachani A., Stinear T.P., Howden B.P., Boyd J.M., Riquelme S.A., Wang C., Prince A., and Wong Fok Lung T., Regulation of airway fumarate promotes S. aureus pneumonia. 8/2025 Nature Communications. PMID: 40745169


45. Boyd J.M., Rios-Delgado G., Esquilín-Lebrón K., Ryan Kaler K., Mereddy G., Norambuena J., Zheng V., Beavers W.N., Kim J., Parker D., Skaar E.P., Carrol. R.K., Yang J.H. Fermentative growth decreases the iron demand of Staphylococcus aureus. 9/2025 Journal of Inorganic Biochemistry. PMID: 41032936

South Dakota State University

46. Celemin Sarmiento, A., B. J. Bradford, L. K. Mamedova, G. Zhou, K. A. Estes, and T. H. Swartz*. 2025. Short Communication: Effects of dietary rumen-protected choline supplementation on genome-wide DNA methylation in mammary tissue from postpartum dairy cows. J. Dairy Sci. Comm. 6:333-338. https://doi.org/10.3168/jdsc.2024-0697

Utah State University

47. Wilson DJ, Goodell GM, Kelly T: Sodium percarbonate added to pasteurized milk for feeding to dairy calves and associated changes in Standard Plate Count over time. Adv Dairy Res 13:1:1-4, 2025


48. Wilson DJ, Goodell GM, Stott RD, Jenson J: Sodium percarbonate added to milk fed to Holstein and Jersey calves and associated outcomes of health, disease or mortality compared with feeding untreated milk. Submitted, in review.


49. Stenkamp-Strahm C, Melody B, McCluskey B, Brinson J, Lombard J. 2025. Influenza A Virus detection in Bulk Tank and Pen Level Milk from Dairies Affected by Highly Pathogenic Avian Influenza H5N1. Journal of Dairy Science. In Review October 2025. Preprint doi: 10.1101/2025.10.26.25338833


50. Stenkamp-Strahm C, McCluskey B, Melody B, Christensen B, Urie N, Amey N, Lomkin R, Campbell A, Lakdawala S, Lombard J. 2025. Dairy Environments with Milk Exposure are Most Likely to Have Detection of Influenza A Virus. Microorganisms. In Review December 2025.Preprint doi: 10.1101/2025.09.03.25335023. PMID: 40950443.

University of Vermont Agriculture Station

51. Machado De Sant’Anna, F., Chakrawarti, A., Haley, B., Barlow, J.W. (2025) “The resistome of pasteurized and raw milk cheeses from the state of Vermont” International Journal of Food Microbiology 441:111333. https://doi.org/10.1016/j.ijfoodmicro.2025.111333


52. Barlow, J.W. (2025) “Antimicrobial Resistance of Mastitis Pathogens of Dairy Cattle” In Mastitis in Ruminants, Veterinary Clinics of North America: Food Animal Practice 41 (2): 223 -236. https://doi.org/10.1016/j.cvfa.2025.02.00


53. Roadcap, E., Lichtenwalner, A., Kennedy-Wade, B., Adjapong, G., Chakrawarti, A., Machado De Sant’Anna, F., Barlow, J.W. (2025) "Whole Genome Sequencing Identifies Exotoxin and Antimicrobial Resistance Profiles of Staphylococcus aureus from Maine Dairy Farms" BMC Veterinary Research 21:154 https://doi.org/10.1186/s12917-025-04630-1

Abstracts

Michigan State University

1. Bacigalupo Sanguesa, P., P.L. Ruegg, S. Manski, R. Thomson, B. Norby, and R.J. Erskine. Exploration of premilking behaviors :  Order of entrance to holding pen and of start of milking.  Meeting Am. Dairy Sci Assoc., June, 2025, Louisville KY.


2. Bacigalupo Sanguesa, P., P.L. Ruegg, S. Manski, R. Thomson, B. Norby, and R.J. Erskine. Associations of pre-milking experience with yield and milk ejection.  Meeting Am. Dairy Sci Assoc., June, 2025, Louisville KY.


3. Strickland, J.M., C. Robison, P.H. Esteves Trinidade, and P.L. Ruegg. Validating use of a 3D scanner for collection of dairy cow udder morphometric data. Ann. Meeting Am. Dairy Sci Assoc., June, 2025, Louisville KY.


4. Juliano, L.C. B, J. Strickland, L. D Souza, I. Buchanna, and P.L. Ruegg, Culling and milk yield characteristics of clinical mastitis cases from large dairy farms that use a no-lactational antibiotic treatment policy. Submitted for Ann. Meeting Am. Dairy Sci Assoc., June, 2025, Louisville KY.


5. Ferreira, L.L.S., T. Showemimo, L. C. B. Juliano, Z. Rodriguez and P.L. Ruegg.  Re-Identification of cryopreserved mastitis pathogens collected from 2003 – 2011 using Maldi-Tof.  64th Annual Meeting National Mastitis Council, Charlotte, NC Jan 2025


6. Strickland, J.M., J. L. de Campos, L. M Sordillo and P.L. Ruegg. The association of blood and milk biomarkers with udder sureface area in cows treated with Pegbovigrastim at dry off.  64th Annual Meeting National Mastitis Council, Charlotte, NC Jan 2025


7. Juliano, L.C.B., and P.L. Ruegg. Preliminary results of characteristics of mastitis occurring on large conventional dairy farms with no-lactational antibiotic treatment policy.  64th Annual Meeting National Mastitis Council, Charlotte, NC  Jan 2025.


8. Goncalves, J.L., Q.K. Kolar, R. Mani, S. Sreevatsan, and P.L. Ruegg. Molecular epidemiology and antimicrobial resistance in Lactococcus spp. isolated from cases of non-severe bovine clinical mastitis.  64th Annual Meeting National Mastitis Council, Charlotte, NC  Jan 2025.


9. Rodriguez, Z; I., Leadbetter, Plosky, A., Robison, C., Martinez, L., Juliano, L., Juarez, A., Marfil, M., Nguyen, U., Paperd, P., Stoutenburg, I., Jimenez, M., Buchanna, I., Summers, E., Wilson, K., Baugh, K., Ruegg, P.L. (2025). “Ongoing efficacy trial of a low-total high free-iodine post-milking teat disinfectant for prevention of new IMI”. Conf. Research Workers Animal Disease, Chicago IL.


10. Varghese, C. Picasso, P.H.E. Trindade, P.L. Ruegg, Z. Rodriguez (2025). “Deep Learning Model for Bacterial Colony Classification & Counting on Agar for Mastitis Diagnostics”. Dairy Continuing Education Day. College of Veterinary Medicine MSU.


11. Varghese, C. Picasso, P.H.E. Trindade, P.L. Ruegg, Z. Rodriguez (2025). “Deep Learning Model for Bacterial Colony Classification & Counting on Agar for Mastitis Diagnostics”. Dairy Continuing Education Day. College of Veterinary Medicine MSU.


12. Plosky, A., Robison, C., Martinez, L., Juliano, L., Juarez, A., Marfil, M., Nguyen, U., Paperd, P., Stoutenburg, I., Jimenez, M., Buchanna, I., Summers, E., Wilson, K., Baugh, K., Ruegg, P.L., Rodriguez, Z (2025). “Bacterial Etiology of Intramammary Infections Across Varying Degrees of Teat-End Hyperkeratosis in Dairy Cows”. Dairy Continuing Education Day. College of Veterinary Medicine MSU.


13. Marino, J. Marfil, R. Simmonds, P.L. Ruegg, Z. Rodriguez, C. Picasso-Risso (2025). “Modeling somatic cell counts in frozen milk samples through predictive adjustment based on storage conditions”. Phi Zeta Research Day. College of Veterinary Medicine MSU


14. Varghese, C. Picasso, P.H.E. Trindade, P.L. Ruegg, Z. Rodriguez (2025). “Deep Learning Model for Bacterial Colony Classification & Counting on Agar for Mastitis Diagnostics”. Phi Zeta Research Day. College of Veterinary Medicine, MSU.


15. Plosky, A., Robison, C., Martinez, L., Juliano, L., Juarez, A., Marfil, M., Nguyen, U., Paperd, P., Stoutenburg, I., Jimenez, M., Buchanna, I., Summers, E., Wilson, K., Baugh, K., Ruegg, P.L., Rodriguez, Z (2025). “Bacterial Etiology of Intramammary Infections Across Varying Degrees of Teat-End Hyperkeratosis in Dairy Cows”. Phi Zeta Research Day, College of Veterinary Medicine, Michigan State University.

University of Minnesota

16. Peña-Mosca, F., Avondet-Benzano, P., Rivera J., Midla, L., Tomazi, T., Florentino, C.C., Caixeta, L.S., Nydam, D.V. “Evaluating the diagnostic utility of on-farm culture for mastitis pathogen group identification on commercial dairy farms.” 58th Annual Conference of the American Association of Bovine Practitioners. Omaha, NE. September 2025.


17. Leonard, E., Wang, S., Crooker, B., Dow, S., Caixeta, L.S., “Using flow cytometry to compare immune cell populations between first lactation dairy cows that did or did not receive immunotherapy at dry-off.” ADSA Conference. Louisville, KY. June 2025.


18. Bonsaglia, E.C.R., Peña-Mosca, F., Rall, V.L.M., Santos, M.V., Lang, K.S., Noyes, N.R., Caixeta, L.S., “Antimicrobial Potential and Genomic Characterization of Bacillus spp. Isolated from Gauze Collected from Teat Skin of Dairy Cows Against Staphylococcus aureus.” ADSA Conference. Louisville, KY. June 2025.


19. Leonard, E., Florentino, C., Ruch, M., Pereira, A., Caixeta , L.S. “Exploratory analysis characterizing the association between late-lactation milk yield or somatic cell count and rectal temperature after dry-off in dairy cows.” ADSA Conference. Louisville, KY. June 2025.


20. Bonsaglia, E.C.R., Peña-Mosca, P., Silva, A.W.S., Gaire, T., Rall, V.L.M., Santos, M.V., Lang, K., Noyes, N.R., Caixeta, L.S. “The inhibitory potential of non – aureus staphylococci isolated from the teat skin of dairy cows against Staphylococcus aureus: a pilot study.” 64th National Mastitis Council Annual Meeting. Charlotte, NC. January 2025.

University of Missouri

21. Byrd B and Adkins PRF. Validating mammary tissue aspirate samples for diagnosis of intramammary infection in dry dairy cattle. Veterinary Scholars Symposium, Spokane, Washington. Aug 7-9, 2025


22. Bakar MA and Adkins PRF. Characterization of virulence genes and antimicrobial resistance among Staphylococcus aureus isolates of dairy origin.  Veterinary Scholars Symposium, Spokane, Washington. Aug 7-9, 2025


23. Adebayo KS and Adkins PRF†. Multi-locus sequence typing (MLST) profiles of Staphylococcus haemolyticus isolates of dairy origin.  Veterinary Scholars Symposium, Spokane, Washington. Aug 7-9, 2025


24. Adesola RO, Adkins PRF, and Barlow JW. Evaluation of strain typing methods used for Staphylococcus chromogenes isolates from dairy farms in the United States. Proceedings of the 64th Annual Meeting of the National Mastitis Council,Charlote NC, Jan 27-30, 2025.


25. Adkins PRF and Byrd B. Induced Staphylococcus chromogenes teat end colonization in dairy heifers. Conference of Research Workers in Animal Diseases. Chicago, IL. Jan 19-21, 2025.

The Ohio State University

26. Oliveria, M. X. S., K. M. Enger, and B. D. Enger. 2025. Influence of intramammary infection and gestational stage on collagen abundance in the mammary gland of primigravid heifers. J. Dairy Sci 108: (Suppl. 1):383.


27. Baker, P. H., L. M. Glendenning, M. X. S. Oliveira, B. A. Cobb, B. D. Enger, and S. N. Langel. 2025. Influenza A virus sialic acid receptor distribution in the bovine mammary gland across physiological states. J. Dairy Sci 108: (Suppl. 1):211.


28. Montgomery, K.R., A.E. Relling, M.X.S. Oliveira, K.M. Enger, S. Wellert, J.E. Coplin, and B.D. Enger 2025. Effect of trace mineral supplement form on mastitis outcomes. J. Dairy Sci 108: (Suppl. 1):195.


29. Oliveira, M.X.S. and B.D. Enger. 2025. Impact of intramammary infection and gestational stage on mammary epithelial cell apoptosis in primigravid dairy heifers. Pages 142-143 in Natl. Mastitis Counc. Annu. Mtg. Proc., Charlotte NC, Natl. Mastitis Council Inc., New Prague, MN.


30. Enger, B.D., M.X.S. Oliveira, P.H. Baker, and K.M. Enger. 2025. Intramammary infection induces mammary epithelial cell death in heifer mammary glands during late gestation. Page 129 in Conf. of Res. Workers in Animal Dis. Proc.

South Dakota State University

31. Reyes, O. D., H. L. Reisinger, I. Brown-Crowder, L. K. Mamedova, T. H. Swartz, and B. J. Bradford. Effects of feeding a Bacillus subtilis (PB6) product on productive performance, metabolic and immunological responses during an intramammary lipopolysaccharide challenge on mid-lactation cows. American Dairy Science Association. Louisville, KY. June 22-25, 2025.


32. Reisinger, H. L., O. D. Reyes, L. K. Mamedova, I. E. Brown-Crowder, T. H. Swartz, and B. J. Bradford. The effects of dietary supplementation of Bacillus subtilis PB6 to mid-lactation dairy cows on neutrophil response following ex vivo bacterial stimulation. American Dairy Science Association. Louisville, KY. June 22-25, 2025.


33. Celemin Sarmiento, A., Bulnes, M., C. Chase, and T. H. Swartz. Effects of systemic inflammation on performance, metabolism, and immune responses during an intramammary Streptococcus uberis challenge in postpartum dairy cattle. American Dairy Science Association. Louisville, KY. June 22-25, 2025.


34. Celemin Sarmiento, A., M. Bulnes, S. Dilberger-Lawson, C. Chase, and T. H. Swartz. Effects of systemic inflammation on inflammatory mediators during an intramammary Streptococcus uberis challenge in postpartum dairy cattle. American Dairy Science Association. Louisville, KY. June 22-25, 2025.


35. Bulnes, M., A. Celemin Sarmiento, T. H. Swartz, and M. E. Uddin. Rumen-derived microbial product supplementation: Effects on feed sorting behavior in dairy cows. American Dairy Science Association. Louisville, KY. June 22-25, 2025.

University of Vermont Agriculture Station

36. Barlow, J., Adrian, R., Chakrawarti, A., Jeffrey, C.E., Mugabi R, Ochoa, A., Machado de Sant’Anna, F., Munk, P. 2025. Case Study: Genetic characterization of methicillin and lincosamide resistant Mammaliicoccus fleurettii isolated from cattle and humans on a Vermont organic dairy farm. Northeast Branch American Society for Microbiology Regional Meeting, Worchester. MA.


37. Machado de Sant'Anna, F., Barlow, J. 2025. Challenges in Detecting and Analyzing ARGs in Cheese: Insights from resistome profiling and methodological comparisons. Northeast Branch American Society for Microbiology Regional Meeting, Worchester. MA.


38. Rai, S. Barlow, J. 2025. Constructing a Bacterial Mock Community Tailored for Teat Skin Microbiome of Dairy Cows. Northeast Branch American Society for Microbiology Regional Meeting, Worchester. MA.


39. Onoh, C.N., Jeffrey, C., Adkins, P., Barlow, J.W. 2025 Comparison of Strain Typing Methods for Staphylococcus chromogenes isolates from Dairy Cattle with Intramammary Infections. Northeast Branch American Society for Microbiology Regional Meeting, Worchester. MA.

Conference Proceedings

Cornell University

1. Vasquez A. 2025. In the Parlor: Practical checks to improve milkout and udder health. Annual New York State Veterinary Conference, Ithaca, NY Oct 10.


2. Vasquez A. 2025. Udderly selective: smarter mastitis treatment strategies. Annual New York State Veterinary Conference, Ithaca, NY Oct 10.


3. Vasquez A. 2025. From teat to tank: troubleshooting high bacterial counts. Annual New York State Veterinary Conference, Ithaca, NY Oct 10.


4. Vasquez A. 2025. Drying off differently: the power of selective dry cow therapy. Annual New York State Veterinary Conference, Ithaca, NY Oct 10.

Long Island University

5. Mahmmod Y, Smit N, Klapproth H, Chaffer M, Zvonar M. Diagnostic Performance of a Novel Molecular Microarray Assay for Diagnosis of Bovine Intramammary Infections with Mycoplasma Species.  64th Annual Meeting National Mastitis Council, Charlotte, NC Jan 2025.

Michigan State University

6. Ruegg, P.L. 2025. Mastitis:  Evidence based treatment of non-severe clinical mastitis. The disease, the Aetiology and the pathogens.  In Proc. Of Cattle Association of Vet. Ireland., Kerry Ireland, Oct 3-5, 2025


7. Ruegg, P.L., 2025. Monitoring, managing and making economically efficient decisions about antibiotic usage on dairy farms.  In Proc. 58th Ann. Conf. Am. Assoc. Bov. Omaha NE,  Sept 11-13, 2025


8. Ruegg, P.L., 2025. State of the Art:  Evidence based treatment of clinical mastitis in dairy cows.  In Proc. Am. College Vet Int. Med.  Louisville, TN, June, 17-18, 2025.


9. Ruegg, P.L., 2025. State of the Art:  Treatment of subclinical mastitis.  In Proc. Am. College Vet Int. Med.  Louisville, TN, June, 17-18, 2025. 

University of Minnesota

10. Bedding management research: What’s next? NE2248 / Mastitis Research Workers Annu. Mtg. Charlotte, NC. Jan 30-31, 2025.


11. Approaches to recycling sand and manure solids bedding with a view to improving udder health. 64th Annu Mtg of the National Mastitis Council. Charlotte, NC. Jan 27-30, 2025


12. Approaches to recycling sand and manure solids bedding with a view to improving udder health. IBA Region V Mini-Regional Meeting. Bloomington, MN 4/8/2025


13. Bedding selection and management: An important determinant in producing quality milk. WI Assoc. of Dairy Plant Field Representatives. 2025 Winter Dairy Education Conference. Middleton, WI 2/4/2025


14. Antimicrobial Use: How much are you spending to control mastitis? GPS Dairy Leaders Forum. Prior Lake, MN 12/4/2024


15. Caixeta, L.S. “Dry-off strategies in the high-producing era boosting health and the bottom line.” BIAH Canada Dairy Advisory Board Meeting. Webinar. October 29th, 2025.


16. Caixeta, L.S. and Vlietstra, C. “Challenges of drying off high-producing cows: what does the research tell us?” National Mastitis Council Pre-conference Short-course. Charlotte, NC. January 27th, 2025


17. Caixeta, L.S. “Manejo do período seco e seus impactos na saúde dos cascos e da glândula mamária” and “Manejo da transição para prevenção de doenças metabólicas e seus impactos em saúde, reprodução e produção”. Elanco Dairy Summit 2024. Ponta Grossa, Brazil and Patos de Minas, Brazil, October., 2024.

The Ohio State University

18. Enger, B.D. and M.X.S. Oliveria. 2025. Mammary development and mastitis risk in pregnant heifers. Pages 53-60 in Natl. Mastitis Counc. Annu. Mtg. Proc., Charlotte NC, Natl. Mastitis Council Inc., New Prague, MN.

Utah State University

19. Wilson DJ, Stott RS, Goodell GM, Jenson J: Sodium percarbonate treatment of milk fed to Holstein and Jersey calves and associated outcomes of health and disease. Mast Res Workers, Chicago, IL, 2025


20. Stenkamp-Strahm C, Melody B, Brinson P, McCluskey B, Campbell AJ, Lakdawala S, Lombard J. Dairy H5N1: Insights from an Early Detection Study of Herds in California. Presented at USAHA/AAVLD, Denver, CO, 2025. Received Scientific Session Oral Presentation Award*

Poster Presentations

Cornell University

1. Vasquez A, Gutierrez-Oviedo F, Farricker M, Ramos V, You M, Jaivaid A, Basnayake V, Senevirante N, Reyes D, and J McFadden. Exploring the relationship between methane intensity and linear score in Holstein dairy cattle. 2025. Annual meeting of the American Association of Bovine Practitioners. Omaha, NE.


2. “Potential application of the IR Biotyper for Strain Typing of Streptococcus uberis in Dairy Herds.” Gioia G, Baccas M, Zurakowski M, Pawloski D, Guarino C. AAVLD. Aurora, CO. October 2025

Long Island University

3. Mahmmod Y, Smit N, Klapproth H, Chaffer M, Zvonar M. Diagnostic Performance of a Novel Molecular Microarray Assay for Diagnosis of Bovine Intramammary Infections with Mycoplasma Species.  64th Annual Meeting National Mastitis Council, Charlotte, NC Jan 2025.

Rutgers University; New Jersey Agricultural Experiment

4. Rios-Delgado G., Boyd J.M., et al., Small RNA IsrR mediates the staphylococcal response to iron limitation. Molecular Biosciences Annual Graduate Student Research Symposium. Rutgers University, New Brunswick, NJ 3/2025


5. Rios-Delgado G., and Boyd J.M., Insight into bacterial adaptation to the hostile host environment. Three-Minute Thesis Competition. Rutgers University, New Brunswick, NJ 3/2025


6. Paluszek S. and Boyd J.M. Mapping interactions between Staphylococcus aureus SUF proteins using a bacterial two-hybrid system. Aresty Research Symposium, Rutgers University, New Brunswick, NJ 4/2025.


7. Chen S. Naidu N., and Boyd J.M. Investigating interactions between Cutibacterium acnes and Staphylococcus epidermidis through spent media. Aresty Research Symposium, Rutgers University, New Brunswick, NJ 4/2025.


8. Rios-Delgado G., and Boyd J.M., Insight into bacterial adaptation to the hostile host environment. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


9. Zheng V., Rios-Delgado G., and Boyd J.M. Small RNA IsrR represses Fe using processes under Fe starvation in Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


10. Paluszek S. and Boyd J.M. Mapping interactions between Staphylococcus aureus SUF proteins using a bacterial two-hybrid system. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


11. Tyagi N., Wong T., and Boyd J.M. Exploring the impact of itaconic acid on the physiology of Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


12. Chen S., Naidu N., and Boyd J.M. Investigating interactions between Cutibacterium acnes and Staphylococcus epidermidis through spent media. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


13. Bovermann H., and Boyd J.M. Decreasing rate of gene expression increases tolerance to copper ions in Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


14. Rios-Delgado G., Zheng V., Ryan Kaler K., Kim J., Zelzion E., Parker D., Lalaouna D., Boyd J.M. Small RNA IsrR mediates the staphylococcal response to iron limitation. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). New Brunswick, NJ 5/2025.


15. Zheng V., Rios-Delgado G., and Boyd J.M. Small RNA IsrR represses Fe using processes under Fe starvation in Staphylococcus aureus. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 5/2025.


16. Tyagi N., Wong T., and Boyd J.M. Exploring the impact of itaconic acid on the physiology of Staphylococcus aureus. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 5/2025.


17. Paluszek S. and Boyd J.M. Mapping interactions between Staphylococcus aureus SUF proteins using a bacterial two-hybrid system. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 5/2025.


18. Chen S., Naidu N., and Boyd J.M. Investigating interactions between Cutibacterium acnes and Staphylococcus epidermidis through spent media. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 5/2025.


19. Bovermann H., and Boyd J.M. Decreasing rate of gene expression increases tolerance to copper ions in Staphylococcus aureus. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 5/2025.


20. Rios-Delgado G., Zheng V., Ryan Kaler K., Kim J., Zelzion E., Parker D., Lalaouna D., Boyd J.M. Small RNA IsrR mediates the staphylococcal response to iron limitation. Rutgers Microbiology symposium, Rutgers University, New Brunswick, NJ 5/2025.


21. Tyagi N., Wong T., and Boyd J.M. Exploring the impact of itaconic acid on the physiology of Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). Camden, NJ 11/2025.


22. Abera T., Bovermann H., Boyd J.M., Changes in iron homeostasis leads to increased sensitivity to copper in Staphylococcus aureus. New Jersey American Society of Microbiology Meeting in Miniature (Theobald Smith Society). Camden, NJ 11/2025.

Outreach Presentations

University of Minnesota

1. Podcast: Bedding selection and management to improve udder health. Dairy Health Blackbelt Podcast. 07/02/2025 https://www.wisenetix.com/blog/Bedding-Management-for-Dairy-Cows


2. Podcast: How much are you spending to control mastitis. GPS DairyCast. 05/27/2025