Annual/Termination Reports:

[10/15/2012] [10/26/2013] [01/08/2015] [06/02/2016]

Report Information

Participants

Alocilja, Evangelyn (alocilja@msu.edu)  Michigan State University; Bhalerao, Kaustubh (bhalerao@illinois.edu) University of Illinois; Bralts, Vincent (bralts@purdue.edu) - Purdue University; Rastogi, Shiva (srastogi@uidaho.edu)  University of Idaho; Su, Winston (wsu1546@gmail.com) - University of Hawaii; Takhistov, Paul (takhistov@aesop.rutgers.edu) - Rutgers University; Yoon, Jeon-Yeol (jyyoon@email.arizona.edu)  University of Arizona; Yu, Chenxu (chenxuyu@iastate.edu) - Iowa State University.

Brief Summary of Minutes

The meeting was held on July 5-6, 2012 at the Kellogg Hotel and Conference Center, Room 105A, Michigan State University, 219 S. Harrison Rd., East Lansing, MI 48824.

First Day, July 5, 2012

At 8:15am on Thursday, July 5, 2012, the meeting was called to order by Dr. Evangelyn Alocilja, 2012 chair of the multi-state group NC-1194, Nanotechnology and Biosensors. The meeting started with introduction of the participants. The group was welcomed to MSU by the following MSU administrators: Dr. Steve Pueppke, Associate Vice President for Research and Graduate Studies, and Director, AgBioResearch; Dr. Satish Udpa, Dean, College of Engineering; and Dr. Ajit Srivastava, Chair, Department of Biosystems and Agricultural Engineering.
Following the welcome, the NC-1194 members presented their progress reports. Questions and discussions of the reports followed each presentation. Discussion covered significance, intellectual merit, and broader impact. Titles of participant presentations are given in Table 1.

After the institutional reports, the group discussed potential multi-institutional collaborations. The group looked at the following opportunities: USDA-NIFA RFPs, Nanotechnology Signature Initiative entitled Nanotechnology for Sensors and Sensors for Nanotechnology, NSF engineering research centers, international collaboration, and instructional collaborations, such as the potential for sharing resources, course materials, and teaching time.
One opportunity for collaboration is joint publication. Dr. Chenxu Yu is guest editing a special issue for the open access journal Biosensors, with a title Nanomaterials for biodetection and drug delivery. He invited the group to submit research papers for this issue. Deadline for paper submission is January 31, 2013.

At 3pm, Dr. Alocilja presented opportunities for technology commercialization and challenged the group to think on how to move their technologies from the lab to the field through effective mechanisms, private-public partnerships, and relevant applications.

At 4pm, Dr. Elliot Ryser (MSU) presented the objectives and mission of the multi-state Enhancing Microbial Food Safety by Risk Analysis. The group discussed with Dr. Ryser potential collaboration between the two multi-state groups.

The group had a working dinner to elect the next set of officers and identify the location of the next meeting. The elected officers for 2013 are:

Chair: Daniel Jenkins

Vice Chair: Jeong-Yeol Yoon

Secretary: Kaustubh Bhalerao

Next meeting: The 2013 annual meeting will be hosted by the University of Hawaii and to be coordinated by Dr. Daniel Jenkins.


Second Day, July 6, 2012

Dr. Alocilja called to order the meeting for the second day, Friday, July 6, 2012. Dr. Douglas Buhler, Interim Dean, College of Agriculture and Natural Resources, was there to welcome the group.
At 8:30am, the group drove to the Cyclotron Building to get a 9am tour of the Cyclotron Facility and the Facility for Rare Isotope Beams (FRIB), a new national user facility for nuclear science which is funded by the Department of Energy Office of Science and the state of Michigan.

At 10:30am, the group visited the Nano-Biosensors Lab in the Department of Biosystems and Agricultural Engineering.

The meeting was adjourned at 12:00 noon.

Table 1. Institutions, participants, and title of presentations.
Rutgers University, Paul Takhistov, Nanoparticles in Food: biosensing, nanostructured materials and transport

University of Illinois at Urbana-Champaign, Kaustubh Bhalerao, Synthetic biology and bionanotechnology

Iowa State University, Chenxu Yu, NanoDEP/SERS-LSPR sensor platform, nano-enabled bactericidal film for food processing, and other technologies

University of Idaho, Shiva K. Rastogi, DNA Detection on Lateral Flow Test Strips: Enhanced Signal Sensitivity Using LNA-conjugated Gold Nanoparticles

University of Hawaii, Wei Wen Su, Tunable nano-oleosomes: biosynthesis, characterization, and applications

University of Arizona, Jeong-Yeol Yoon, Development of handheld cell-phone based biosensor and microfluidic devices

Michigan State University, Evangelyn Alocilja, Nano-Biosensors for Global Health, Bio-defense, Food Safety, and Water Quality

Accomplishments

This is a progress report for the period October 1, 2011-September 30, 2012 by participating institutions covering various states. As a reminder, the objectives of this project are:<br /> 1. Develop new technologies for characterizing fundamental nanoscale processes<br /> 2. Construct and characterize self-assembled nanostructures<br /> 3. Develop devices and systems incorporating microfabrication and nanotechnology<br /> 4. Develop a framework for economic, environmental and health risk assessment for nanotechnologies applied to food, agriculture and biological systems<br /> 5. Produce education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment<br /> Progress reports are presented by state. The findings have been disseminated to the scientific community via seminars, national/international conferences, manuscripts, and web sites.<br /> <br /> Arizona (University of Arizona)<br /> Accomplishments from the University of Arizona were presented at the meeting by Dr. Jeong-Yeol Yoon (Table 1). For details, please refer to http://biosensors.abe.arizona.edu/index.html.<br /> <br /> Idaho (University of Idaho)<br /> Accomplishments from the University of Idaho were presented at the meeting by Dr. Shiva Rastogi (Table 1). For details, please refer to http://www.uidaho.edu/cals/news/feature/rastogi.<br /> <br /> Illinois (University of Illinois at Urbana-Champaign)<br /> Accomplishments from the University of Illinois at Urbana-Champaign were presented at the meeting by Dr. Kaustubh Bhalerao (Table 1). For details, please refer to http://abe-bhaleraolab.age.uiuc.edu/members/kbhalerao/<br /> <br /> Indiana (Purdue University)<br /> Output. In the reporting year, principal investigators at Purdue University made advances in the area of nanotechnology and biosensors. These advances include novel and improved technologies for both intracellular and extracellular measurements of dynamic biomolecule events within cells. Fundamental understanding and design of material properties and behavior at the nanoscale fueled these technologies. These properties include electrochemical, optical, and biological behavior of carbon nanotubes, nanostructures platinum, mesoporous silica, platinum nanoparticles, and gold nanoparticles and probes. These physiological sensing technologies have a wide range of applications and impact in areas including environmental toxicology and monitoring, metabolic disease including diabetes, cancer, water monitoring and regeneration, and food.<br /> <br /> Iowa (Iowa State University) <br /> Accomplishments from Iowa State University were presented at the meeting by Dr. Chenxu Yu (Table 1). For details, please refer to http://www.abe.iastate.edu/abe-department/directory/chenxu-yu/. A written report is also presented below.<br /> Output. The activities during this period included: (1) further development of dual-recognition Raman sensing scheme for single step pathogen detection in a lab-in-a-tube platform, detection limit is improved to 100 CFU/mL, with a multiplex target identification scheme. (2) The development of nanophotocatalyst for pretreatment of biomass to improve its digestibility for biofuel/biorenewables production. (3) The development of Raman spectroscopic imaging-based characterization of glaucomatous retinal tissues. The Raman imaging technique may serve as the basis for a method to diagnose glaucoma at an early stage, which will greatly benefit glaucoma patients. (4) The development of a nano-functionalized antimicrobial biodegradable coating for food safety control. (5) The development of a rapid UV spectroscopic detector for monitoring trace amount of egg yolk contamination in egg white. These findings were presented in several peer reviewed conferences and three journal articles.<br /> Impact. The spectroscopic sensing techniques developed in this project are highly accurate, they have the potential to meet the needs in various disciplines for high-accuracy target sensing, including early diagnosis of diseases (i.e., glaucoma), and rapid screening for foodborne pathogens. The utilization of nanophotocatalyst to pretreat biomass with reduced water and chemical usage, which will make biofuel and biorenewable production more environmentally friendly and less costly. The development of a nano-functionalized antimicrobial biodegradable coating for food safety control utilizes biodegradable films made from soy and corn proteins, and functionalizes them with antimicrobial nanoparticles to improve their mechanical strength and to impart in the film antimicrobial functionality. The goal is to create cheap coating materials that are antimicrobial and can be used in food processing/food handling places to improve food safety, such as fresh produce and meat. The UV spectroscopic sensor for egg yolk contamination in egg white addresses an important industrial need. It can detect 0.001% yolk contamination in egg white in less than 5 seconds.<br /> <br /> Hawaii (University Hawaii)<br /> Accomplishments from the University Hawaii were presented at the meeting by Dr. Wei Wen Su (Table 1). For details, please refer to http://www2.hawaii.edu/~wsu/. A written report is also presented below.<br /> Output. Research for the project has focused on the development of technologies enabling rapid gene-based agricultural diagnostics directly in the field. Activities/outputs include: (1) A new molecular probe technology Assimilating Probes was developed which was the first to enable real-time quantitative detection using an isothermal gene based amplification LAMP, and which can be used for multiplexed detection of multiple sequences or the incorporation of an internal positive control to validate test performance. Primer probe sets have been developed for several pathogens, including Ralstonia solanacearum and Salmonella enterica. (2) A single-test handheld device was developed for rapid field detection using LAMP and assimilating probes. Devices, interfaced by Bluetooth with a tablet or smart-phone, were demonstrated in the field at a remote village in Guatemala for the detection of select agent race 3 biovar 2 strains of the bacterial wilt pathogen Ralstonia solanacearum. (3) A multiplexed LAMP/ Assimilating Probe assay was developed to classify race 3 biovar 2 strains of Ralstonia solanacearum, allowing discrimination of these quarantine strains from more ubiquitous and less harmful strains of the pathogen. (4) A second generation handheld device was developed capable of running 8 simultaneous test reactions, with two optical channels for detection of two specific sequences in each reaction. This design is currently being refined into a commercial-ready device suitable for sale to researchers, food producers and processors. (5) Work has been initiated on a new method for screening large volumes of agricultural materials (rinsates, irrigation water, soil, plant and animal tissues) to effectively isolate disperse zero-tolerance pathogens such as Salmonella and lethal E. coli strains. Results have been disseminated to scientific communities and to the public through a variety of publications and conference presentations. <br /> Impact. The technologies developed in this research are intended to allow rapid detection of agricultural and food-borne pathogens to improve food security and safety. Handheld rapid detection has been identified as a critical need by the USDA for enhancing food safety and implementing effective surveillance programs to exclude harmful pathogens from agricultural settings. We have initiated collaborations with a number of USDA-ARS research groups to adapt our technologies for serious food-pathogens, devastating quarantine pathogens, and other pest organisms to facilitate more effective/ economical pest management.<br /> In the 2011 annual project meeting, commercialization of sensor technologies was identified as being a critical activity as the technologies themselves are rapidly reaching maturity. As such, we have focused significant efforts in commercializing inexpensive devices and gene-based test kits that can be a powerful tool for food growers and processors.<br /> <br /> Michigan (Michigan State University)<br /> Accomplishments from Michigan State University were presented at the meeting by Dr. Evangelyn Alocilja (Table 1). For details, please refer to http://www.egr.msu.edu/~alocilja/. A written report is also presented below.<br /> Output. Research accomplishments at Michigan State University include the development of a green chemistry synthesis of gold nanoparticles, development of nanoparticle-based biosensors, validation of these biosensors in various food matrices, and effective immunomagnetic extraction techniques. Specifically, we have developed biosensors based on biobarcode, electrospun nanofiber, nonwoven fibers, polyaniline label, and copolymer mass amplification. We have also developed a method for directly detecting genomic DNA from Salmonella enteritidis without PCR amplification. <br /> Impact. In January 2012, Dr. Alociljas nano-biosensor technologies have been licensed to nanoRETE, Inc., a start-up company established to commercialize her technologies. The company is backed by Michigan Accelerator Fund I, a venture capital investment group based in Grand Rapids, Michigan. Dr. Alocilja is the companys founder and Chief Scientific Officer. The company is located in Lansing, MI. With 4 full time scientists and 3 part-time researchers and administrators, the company is already contributing to job creation in Michigan.<br /> <br /> Minnesota (University Minnesota)<br /> Output. The main problems in the dental implant industry are: (1) formation of biofilm around the implant  a condition known as peri-implantitis, and (2) inadequate bone formation around the implant  lack of osseointegration. Therefore, developing an implant to overcome these problems is of significant interest. Chitosan has been reported to have good biocompatibility and also anti-bacterial activity. An osseo-inductive recombinant biopolymer (P-HAP), derived from the protein statherin has been reported to induce bone formation. We have deployed these two materials to build assembled layer-by-layer (LbL) coatings on titanium by exploiting their opposite charges in appropriate conditions. The LbL coatings were characterized by contact angle measurement, FTIR and AFM. The substantial difference in water contact angle between alternate layers, the representative peaks in FTIR & XPS, and the changes in topography between surfaces with different number of bi-layers observed in AFM indicated that we successfully built-up the chitosan/P-HAP LbL. The LbL samples showed increased amount of biomineralization in osteogenic media (OS) supplemented with CaCl2, which is attributed to the well-established preferential interaction of statherin with mineral in teeth. The osteoblast cells line (MC3T3) adhered well to the constructed bi-layers, but did not quite differentiate well on the bi-layers. Since the biomineralization increased with the layer buildup, these biomineralized samples can be further explored to assess their biological activity - cell adhesion, proliferation and differentiation of osteoblasts and antimicrobial - inhibition of attachment and growth of oral pathogens in vitro potential.<br /> <br /> New Jersey (Rutgers University)<br /> Accomplishments from Rutgers University were presented at the meeting by Dr. Paul Takhistov (Table 1). For details, please refer to http://foodsci.rutgers.edu/takhistov/.<br /> <br /> New York (Cornell University) <br /> Output. We have worked on the development of a number of different platforms over the course of the last year. This includes microfluidic channels for the isolation and amplification of RNA molecules of the protozoan parasite Cryptosporidium parvum; transfer of the technology to a commercial microfluidic system in collaboration with Rheonix, Inc., by investigating the possibility of using biofunctional electrospun nanofibers directly in microfluidic channels; by integrating a pre-concentration step on-chip; and by investigating novel signaling strategies for lab-on-a-chip devices based on electrochemiluminescence. <br /> Impact. We have developed a minipotentiostat that can control a 3-electrode system and can be used for electrochemiluminescence detection. We have determined that nanovesicles and cells can be specifically captured on appropriate nanofiber surfaces and can be released using pH shifts. We have demonstrated that pre-concentration prior to detection on-chip can lower the limit of detection by more than one order of magnitude. <br /> Over the past decade, rapid point-of-care (POC) tests have emerged as one of the largest and fastest growing segments of the human in vitro diagnostic testing market and can also be found in food safety applications. Due to their relative ease, most such tests rely upon immunologic methods. A common testing format employs specific antibodies that recognize and interact with the analytes of interest in a manner that will also induce the generation of a discernable color within a lateral flow device. Such tests are frequently designed to yield qualitative (i.e., yes or no) results rather than quantitative tests. Despite the added information that can be obtained via molecular detection methods, similar rapid tests employing gene detection methods have not yet found widespread use, which is primarily due to the more complex and time-consuming nature of sample preparation methods as well as the more technically complicated gene amplification techniques. <br /> The approach developed by us will greatly ameliorate the situation since it combines a quantitative approach with the highly selective nucleic acid recognition of pathogenic organisms and simplifies the amplification techniques. Thus, pathogens will be detectable and quantifiable at significantly lower costs, and tests will be more reliable since fewer assay steps are needed.<br /> <br /> South Carolina (Clemson University)<br /> Output. Adherence is among the earliest event in many bacterial infections. These interactions are required for extracellular colonization and/or internalization. This colonization is usually mediated by bacterial adhesins on the surface of bacteria that recognize and bind to specific receptor moieties of host cells. This adhesin-receptor binding event could activate cascades of signal transduction important in the pathogenic process and host-defense. Understanding of this adhesin-receptor interaction is of great value in order to develop effective prevention, detection, diagnostic, and treatment methods.<br /> Nanotechnology offers new opportunities for the nanoscale investigation of adhesin-receptor interactions. We envision that adhesin specific nanoparticles and biosensors will break new ground in study of pathogen-host interactions, drug discovery, drug delivery, and biosensing. To achieve such goals, the fundamentals of adhesin-receptor interactions must be examined.<br /> In this present work, the PI and his team have functionalized nanoparticles with receptor molecules recognized by specific bacterial adhesins to demonstrate that such nanoparticles could be utilized to eliminate pathogens from host without the need for antibiotics that many pathogens are now resistant to. The PI and his team have also utilized the same principle to develop highly sensitive biosensors capable of detecting the targeted pathogens in low number. The PI has received a resource grant from the Consortium for Functional Glycomics allowing the PI to tap into their carbohydrate resources enabling the PI to functionalize various nanomaterials for the proposed studies.<br /> The PI and his team have also fabricated several nanomaterials/devices to study nanoscale phenomena for applications in pathogen detection/inactivation and risk assessment. <br /> Impact. The use of carbohydrates as capturing agents in biosensor development represents a more specific, stable, and economical approach than the conventional immunoassays or PCR detection methods that are more costly and incapable of differentiating non-viable from viable targets.<br /> <br /> Wisconsin (University of Wisconsin)<br /> Output. We are investigating how a fundamental nanotechnological phenomenon  plasmonic effect of in situ synthesized gold nanoparticles (AuNPs)  can be used to develop a novel system to provide visible indication of thermal history of foods, which will signal the need for prompt and timely food safety intervention. Formation of AuNPs can mimic temperature-driven changes in bioproducts by using functional biomolecules (proteins/polysaccharides) as mediate materials, because the specific characteristics of the biomolecules dictate the formation of AuNPs. Gelatin is an edible protein derived from collagen. Due to its low cost and unique ability to form thermo-reversible hydrogel, gelatin is used in a myriad of practical applications including as a food and as stabilizing agent in foods. Gelatin has been employed for the synthesis of metal NPs. The outstanding transparency of gelatin gels is also highly valued, especially by the photographic film industry for colorimetric applications. Thus, gelatin is an excellent material for both mediating the formation of AuNPs and representing biomaterials. We are studying AuNPs synthesis in the presence of gelatin as a potential system for food material thermal history by investigating the effect of temperature and temperature history on the AuNP synthesis.<br /> Impact. Our system will help ensure the safety and security of our food chain before foods contaminated with pathogenic organisms and/or toxins are distributed and consumed.<br /> <br /> Work plan for the next year:<br /> Research by each investigator at each participating institution will continue as funding becomes available. New techniques will be developed for characterizing fundamental nanoscale processes. Self-assembled nanostructures will be designed and characterized. New biosensor devices and systems will be developed and will continue to incorporate microfabrication and nanotechnology. Economic frameworks and environmental and health risk assessments of nanomaterials as applied to food, agriculture and biological systems will become a priority. And finally, educational and outreach materials on nanofabrication, sensing, systems integration and application risk assessment will be developed.<br />

Publications

Anderson, J. M., Torres-Chavolla, E., Castro, A. B., and Alocilja, E.C. 2011. One step alkaline synthesis of biocompatible gold nanoparticles using dextrin as capping agent. Journal of Nanoparticle Research, 13(7), 2843-2851. DOI 10.1007/s11051-010-0172-3.<br /> <br /> Anderson, M.J., Fisher, A.R., Ly, A., Alocilja, E.C., Smith, C.B. 2012. Rapid Detection of Escherichia coli O157:H7 Using Competitive Exchange of Fluorescent Surrogate Modified Surfaces in Liquid Media. Sensors & Transducers, 137(2), 254-262.<br /> <br /> Anderson, M.J., Zhang, D., and Alocilja, E.C., 2011. Spectral and electrical nanoparticle-based molecular detection of Bacillus anthracis using copolymer mass amplification. IEEE Transaction on Nanotechnology, 10(1), p. 44-49 (January 2011). http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5523957&tag=1<br /> <br /> Chenxu Yu, Spectroscopic Biosensing for food quality and safety control, Jiangxi Agricultural University, Invited talk, (May 28, 2012)<br /> <br /> Cho, D., Matlock-Colangelo, L.; Xiang, C.; Asiello, P. J.; Baeumner, A. J.; Frey, M. W., Electrospun nanofibers for microfluidic analytical systems. Polymer available online. http://dx.doi.org/10.1016/j.polymer.2011.05.026 <br /> <br /> David Jaroch, Eric McLamore, Wen Zhang, Jin Shi, Jay Garland, M. Katherine Banks, D. Goddard, J., Mandal, S., Nugen, S., Baeumner, A., Erickson, D. Patterning of Nucleic Acid Probes in Optical Nanocavities, Colloids and Surfaces B: Biointerfaces (2010) vol. 76, pp. 375380, doi:10.1016/j.colsurfb.2009.10.041)<br /> <br /> Guo, C. and Irudayaraj, J. 2011. Fluorescent Ag clusters via a Protein-directed approach as a Hg(II) ion sensor. Analytical Chemistry. 83(8):2883-9.<br /> <br /> Guo, C., Book, B., and Irudayaraj, J. 2011. Protein-directed reduction of Graphene oxide and intracellular imaging. Chemical Communications. 47(47):12658-12660.<br /> <br /> Jenkins, D. M., Kubota, R., J. Dong, Y. Li, and D. Higashiguchi. 2011. Handheld device for real-time, quantitative, LAMP-based detection of Salmonella enterica using assimilating probes. Biosensors & Bioelectronics. 30(1):255-260.<br /> <br /> K. Yang, D. M. Jenkins, and W.-W. Su. 2011. Rapid concentration of bacteria using submicron magnetic ion exchangers for improving PCR-based multiplex pathogen detection. Journal of Microbiological Methods. 77(3):182-193.<br /> <br /> Kadam, D. M, Thunga, M., Srinivasan, G., Grewell, D., Kessler, M., Chenxu Yu, Lamsal, B, Mechanical and Microstructural Properties of Nano Functionalized Corn Zein Protein and Whey Protein Isolate Biopolymers, Biopolymers & Bio composites Workshop, Ames, (August 13-15, 2012) <br /> <br /> Kadam, D. M, Thunga, M., Wang, C., Grewell, D., Kessler, M., Chenxu Yu, Lamsal, B, Protein based nano-functionalized antimicrobial films and their properties, 11th Conference of Food Engineering CoFE 2012, Leesburg, (April 1-4, 2012)<br /> <br /> Kamikawa, T.L., Mikolajczyk, M.G., Kennedy, M., Zhong, L., Zhang, P., Setterington, E., Scott, D.E., and Alocilja, E.C., 2012. Pandemic influenza detection by electrically active magnetic nanoparticles and surface Plasmon resonance. IEEE Transactions on Nanotechnology, 11(1), 88-96. DOI: 10.1109/TNANO.2011.2157936 <br /> <br /> Kondapalli, S., Connelly, J.T., Baeumner, A.J., Kirby, BJ. Integrated microfluidic preconcentrator and immunobiosensor Microfluid Nanofluid (2011) DOI 10.1007/s10404-011-0819-0 <br /> <br /> Kubota, R., A. M. Alvarez, and W.-W. Su, D. M. Jenkins. 2011. Fret-based assimilating probe for sequence specific real-time monitoring of Loop Mediated isothermal AMPlification. Biological Engineering Transactions. 4(2):81-100.<br /> <br /> Kubota, R., P. LaBarre, J. Singleton, A. Beddoe, B. H. Weigl, A. M. Alvarez, and D. M. Jenkins. 2011. Non-instrumented nucleic acid amplification (NINA) for rapid detection of Ralstonia solanacearum race 3 biovar 2. Biological Engineering Transactions. 4(2):69-80.<br /> <br /> Kubota, R., P. LaBarre, J. Singleton, A. Beddoe, B. H. Weigl, A. M. Alvarez, and D. M. Jenkins. 2012. Molecular diagnostics in a teacup: non-instrumented nucleic acid amplification (NINA) for rapid, low cost detection of Salmonella enterica. Chinese Science Bulletin. (Invited Manuscript for Special English Issue- in Press).<br /> <br /> Lee, K., Drachev, V., and Irudayaraj, J. 2011. DNA-Gold nanoparticle networks grown at cell surface marker sites: Diagnostics of cancer stem cells. ACS NANO. 5(3):2109-2117.<br /> <br /> Lim S, S Gunasekaran, J-Y Imm. 2012. Gelatin-templated gold nanoparticles as novel time-temperature indicator. J of Food Science 77 (9):N45-49.<br /> <br /> Luo, Y., Nartker, S., Wiederoder, M., Miller, H., Hochhalter, D., Drzal, L.T., and Alocilja, E.C., 2012. Novel Biosensor based on Electrospun Nanofiber and Magnetic Nanoparticles for the Detection of E. coli O157:H7. IEEE Transactions on Nanotechnology, 11(4),676-681. http://ieeexplore.ieee.org/stamp/stamp.jsp?arnumber=06082451<br /> <br /> Marshall Porterfield, Jenna L. Rickus. (2011) Cell-Mediated Deposition of Porous Silica on Bacterial Biofilms. Biotechnology and Bioengineering. Vol. 108. Issue 10. 2249-2260. Editors Choice Selection.<br /> <br /> McGraw, S., Anderson, M., Alocilja, E.C., Marek, P.J., Senecal, K.J., and Senecal, A.G. 2011. Antibody Immobilization on Conductive Polymer Coated Nonwoven Fibers for Biosensors. Sensors and Transducers, 13, 142-149.<br /> <br /> McLamore, E.S., J. Shi, D. Jaroch, J.C. Claussen, A. Uchida, Y. Jiang, W. Zhang, S.S. Donkin, M.K. Banks, K.K. Buhman, D. Teegarden, J.L. Rickus, and D.M. Porterfield. (2011) A self-referencing platinum nanoparticle decorated enzyme-based microbiosensor for real time measurement of physiological glucose transport. Biosensors and Bioelectronics. 26(5): p. 2237-45.<br /> <br /> Mura, S., Corrias, F., Stara, G., Piccinini, M., Secchi, N., Marongiu, D., Innocenzi., P., Irudayaraj, J., and Greppi, G. 2011. Innovative composite films of chitosan, methylcellulose and nanoparticles. Nanoscale Food Sci., Engineering & Technology. J. Food Science. 76(7):N54-N60. <br /> <br /> Nitkowski, A., Baeumner A.J., Lipson, M. On-chip spectrophotometry for bioanalysis using microring resonators Biomedical Optics Express, vol. 2(2), pp. 272  277 (2011)<br /> <br /> Packard, M.M. and Alocilja, E.C. 2012. High-throughput assay for real-time detection and quantification of low concentration, viable Escherichia coli O157:H7 in apple juice. Food Science and Technology Letters, 3(1), 20-23. <br /> <br /> Panchapakesan, B., Book, B., Seth R., and Irudayaraj, J. 2011. Gold nanoprobes for theragnostics and diagnostics. Journal of Nanomedicine, 6(10):1-7.<br /> Parul Pal, & Shiva K. Rastogi et al. ACS Appl. Mater. and Interfaces, 2011, 3, 279 [4.525] <br /> <br /> Patel, S., D. Showers, P. Vedantam, T.-R. Tzeng, S. Qian, and X. Xuan, (2012). Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis, Biomicrofluidics, Epub ahead of print, Vol. 6, Issue 3<br /> <br /> Ravindranath, S., Henne, K., Thompson, D., and Irudayaraj, J. 2011. Surface-enhanced Raman Imaging of Intracellular Bioreduction of Chromate in Shewanella oneidensis. PLoS ONE. 6(2): e16634 (10 pages).<br /> <br /> Ravindranath, S., Henne, T., Thompson, D., and Irudayaraj, J. 2011. Intracellular bioreduction of Chromate-decorated Gold nanoparticles by <br /> <br /> Shewanella oneidensis MR-1 by Raman chemical imaging. ACS NANO. 5(6):4729-36.<br /> Ravindranath, S., Wang, Y., Irudayaraj, J. 2011. SERS driven Cross-platform based multiplex pathogen detection. Sensors & Actuators: B. Chemical. 152: 183-190.<br /> <br /> Setterington, E. and Alocilja, E.C. 2012. Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens. Biosensors, 2, 15-31. doi:10.3390/bios2010015<br /> <br /> Setterington, E.B., Cloutier, B.C., Ochoa, J.M., Cloutier, A.K., Jain, P., Alocilja, E.C. 2011. Rapid, sensitive, and specific immunomagnetic separation of foodborne pathogens. International Journal of Food Safety, Nutrition and Public Health, 4(1):83-100.<br /> <br /> Setterington, E.H. and Alocilja, E.C., 2011. Rapid electrochemical detection of polyaniline-labeled Escherichia coli O157:H7. Biosensors and Bioelectronics, 26(5), 2208-2212 (http://dx.doi.org/10.1016/j.bios.2010.09.036).<br /> <br /> Shi, J,; Claussen, JC; McLamore, ES; ul Haque, A; Jaroch, D; Diggs, AR; Calvo-Marzal, P; Rickus, JL; Porterfield, DM. (2011) A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors. Nanotechnology. Vol. 22, Issue 35.<br /> <br /> Shi, J., E. McLamore, D. Jaroch, J. Claussen, J. Rickus, and D.M. Porterfield. (2011) Oscillatory glucose flux in INS1 pancreatic beta cells: A self-referencing microbiosensor study. Analytical Biochemistry Vol 411. 185-193. <br /> <br /> Shiva K. Rastogi et al. ACS Appl. Mater. and Interfaces, 2011, 3, 1731 [4.525] <br /> <br /> Shiva K. Rastogi et al. J. of Nanopart. Res., 2012, 14, 673; [3.287] <br /> <br /> Shiva K. Rastogi et al. Nanomedicine: NBM, 2011, 7, 305 [6.692] <br /> <br /> Shiva K. Rastogi et al. Sensors & Transducers Journal, 2009, 7, 191 <br /> Srinivasan, S., Aslan, A., Xagoraraki, I., Alocilja, E., Rose, J.B. 2011. Escherichia coli, enterococci, and Bacteroides thetaiotaomicron qPCR signals through wastewater and septage treatment. Water Research, 45, 2561-2572.<br /> <br /> Sun, L., Chenxu Yu, J. Irudayaraj, Profiling slternative splicing in breast cancer cells by surface enhanced Raman spectroscopy, Nano-2012, International Conference and exhibition on nanotechnology and nanomedicine, Omaha (March 12-14, 2012)<br /> <br /> Tae-Gon Cha, Benjamin A. Baker, M. Dane Sauffer, Janette Salgado, David Jaroch, Jenna L. Rickus, D. Marshall Porterfield, and Jong Hyun Choi. (2011) Optical Nanosensor Architecture for Cell-Signaling Molecules Using DNA Aptamer-Coated Carbon Nanotubes. ACS Nano. 5 (5), pp 42364244<br /> <br /> Torres-Chavolla, E., and Alocilja, E.C. 2011. Nanoparticle based DNA biosensor for tuberculosis detection using thermophilic helicase-dependent isothermal amplification. Biosensors and Bioelectronics, 26(11), 4614 4618 (July 2011), http://dx.doi.org/10.1016/j.bios.2011.04.055<br /> <br /> Tzeng, T.-R., Y.R. Cheng, R. Saeidpourazar, S.S. Aphale, N. Jalili, (2011). Adhesin-Specific Nanomechnical Cantilever Biosensors for Detection of Microorganisms, Journal of Heat Transfer, 133, Issue 1<br /> <br /> Vedantam, P., T.-R. Tzeng, (2012). Binding of Escherichia coli to functionalized gold nanoparticles. Plasmonics, Epub ahead of print, Vol. 7, Number 2, 301-308<br /> <br /> Vetrone, S.A., Huarng, M.C., and Alocilja, E.C. 2012. Detection of Non-PCR Amplified S. enteritidis Genomic DNA from Food Matrices Using a Gold-Nanoparticle DNA Biosensor: A Proof-of-Concept Study. Sensors, 12, 10487-10499; doi:10.3390/s120810487<br /> <br /> Wang, C., Chenxu Yu, Detection of chemical pollutant in water using gold nanoparticles as sensors: a review, Reviews in Analytical Chemistry, in press<br /> <br /> Wang, C., Yoo, C., Chenxu Yu, Kim, T., Photocatalyst aided alkaline pretreatment and Raman spectroscopic characterization of corn stover biomass, ASABE annual meeting, Dallas, (July 29-August 3, 2012)<br /> <br /> Wang, Q. and Chenxu Yu, Chapter 3. Chemical and Biological Sensing and imaging using plasmonic nanoparticles and nanostructures in Biomedical nanosensors, edited by J. Irudayaraj, pp. 59-96, Pan Stanford Publishing Pte. Ltd., 2012<br /> <br /> Wang, Q., S. Lonergan and Chenxu Yu, Rapid determination of pork sensory quality using Raman spectroscopy, Meat Science, 91, 232-239, 2012<br /> <br /> Wang, Y. and Irudayaraj, J. 2011. A SERS DNAzyme biosensor for lead ion detection. Chemical Communications. 47:4394-4396.<br /> Wang, Y., Chen, J., and Irudayaraj, J. 2011. Nuclear targeting dynamics of gold nanoclusters for enhanced therapy of HER2+ breast cancer. ACS NANO. 5(12):9718-9725.<br /> <br /> Xuan, P., Y. Zhang, T.-R. Tzeng, X.F. Wan, F. Luo, (2012). A quantitative structure-activity relationship (QSAR) study on glycan array data to determine the specificities of glycan-binding proteins, Glycobiology, Vol. 2, Issue 4, 552-560<br /> <br /> Yoo, C., C. Wang, Chenxu Yu, B. Lamsal and T. Kim, Improved enzymatic hydrolysis of corn tover by nano photocatalyst aided ammonia pretreatment, AIChE Annual meeting, Minneapolis (October 16-12, 2011)<br /> <br /> Zeng, J., C. Chen, P. Vedantam, V. Brown, T.-R. Tzeng, X. Xuan, (2012). Three-dimensional magnetic focusing of particles and cells in ferrofluid flow through a straight microchannel, Micromechanics and Microengineering, in press<br /> <br /> Zhang, D., Anderson, M.J., Huarng, M.C., and Alocilja, E.C. 2011. Nanoparticle-based Bio-barcoded DNA Sensor for the Rapid Detection of pagA Gene of Bacillus Anthracis. IEEE Transactions on Nanotechnology,10(6), 1433-1438. <br /> <br /> Zhu, B., D. Zhou, J. Yang, D. Li, Y. Murata and Chenxu Yu, Effect of thermal treatment on the texture and Microstructure of Abalone muscle (Haliotis discus), Food science and biotechnology, 20(6), 1467-1473, 2011.<br /> <br /> Zhu, B., X. Dong, Y, Gao, D. Zhou, J. Yang, D. Li, X. Zhao, T. Ren, W. Ye, H. Tan, Y. Murata and Chenxu Yu, Physicochemical properties and radical scavenging capacities of pepsin-solubilized collagen from sea cucumber Stichopus japonicus, Food Hydrocolloids, 28, 182-188, 2012<br /> <br /> Zhu, J., R.C. Canter, G. Keten, P. Vedantam, T.J. Tzeng, and X. Xuan, (2011). Continuous-flow particle and cell separations in a serpentine microchannel via curvature-induced dielectrophoresis, Microfluidics and Nanofluidics, 11, 743-752<br /> <br />

Impact Statements

1. The technologies developed in this multi-state collaboration are designed to improve food safety, water quality, agricultural production, and health. Handheld biosensor devices utilizing nanomaterials and techniques are the major contributions in this report. Furthermore, transfer of some of the technologies to the private sector has generated jobs and will contribute to the economic progress of the country and respective states.

Back to top

Report Information

Participants

Alocilja, Evangelyn (alocilja@msu.edu)  Michigan State University;
Bhalerao, Kaustubh (bhalerao@illinois.edu) University of Illinois;
Bralts, Vincent (bralts@purdue.edu) - Purdue University;
Chen, Hongda (HCHEN@nifa.usda.gov) - USDA NIFA
Gunasekaran, Sundaram (guna@wisc.edu) University of Wisconsin;
Hancock, Adam - University of Kentucky;
Hu, Wuyang (wuyang.hu@uky.edu) - University of Kentucky;
Jenkins, Daniel (danielje@hawaii.edu) - University of Hawaii;
Takhistov, Paul (takhistov@aesop.rutgers.edu) - Rutgers University;
Zhou, Anhong (Anhong.Zhou@usu.edu) - Utah State University.

Brief Summary of Minutes

See attached "Copy of Minutes" file attached below for full annual report.


Brief summary of minutes of annual meeting:
The meeting was held on May 12 - 13, 2012 in the Executive Boardroom at the Westin National Harbor (171 Waterfront St., National Harbor, MD 20745).
First Day, May 12, 2013
Meeting called to order by Daniel Jenkins at 1:06 PM, who thanked Evangelyn Alocilja for taking the initiative to organize this year's meeting in conjuction with the 2013 TechConnect World Summit and Innovation Showcase.
1) Following introductions of participants, Vincent Bralts (administrative advisor) welcomed attendees and recognized the great opportunity to participate in the ongoing Nanotechnology Expo.
2) Hongda Chen (from USDA-NIFA) welcomed participants, and reviewed emerging opportunities from USDA and other federal agencies, including nanotechnology programs funded through partnerships at multiple federal agencies totalling $78M which would fit the objectives of NC-1194 well. Specific programs are related to nanotechnology and sensors to improve performance, understand fate of nanomaterials in the environment, improve decision support technologies for precision agriculture, and nanoscale modeling. He went on to review some other issues including budgetary uncertainties due to federal sequestration,
3) Kaustubh Bhalerao indicated that it would be a good idea to try to consolidate NC-1194 with NECC1014 (Nanotechnology Risk Assessment) which has significant overlap of objectives with NC-1194. Vincent Bralts agreed to serve as a delegate to NECC1014 to invite them to merge with NC-1194.
4) Some discussion ensued about the benefits of coordinating meetings with larger Meetings/ Conferences (as was done this year). Participants recommended that meeting venues should alternate every other year between different state experiment stations and larger meetings and conferences, to balance the need to understand the needs of individual states, and the need for keeping current with other professionals, technologies, and issues. It was decided that next year's meeting will be hosted by the University of Hawaii, and meetings will be coordinated with larger meetings/ conferences in every odd numbered year.
5) Alocilja started a short dialog to stimulate ideas for NC-1194 to have more participation, collaborative activities, and impact (this dialog was resumed in the evening's "business" dinner held at the Thai Pavilion Restaurant in National Harbor MD). Two ideas were received quite enthusiastically, including: i) development of a high-performance/ low-cost/ open source electrochemical impedance analyzer/ potentiostat to support projects by many project members as well as other researchers and companies (suggested by Kaustubh Bhalerao, who already has some momentum in developing such a device), and; ii) collaborating on a review paper on electroanalytical chemistry for biological applications (suggested by Paul Takhistov, as again this topic appeared to be the most common theme emerging in station reports, and documenting a common basic framework for these technologies would have a very positive impact).
6) Short break, followed by Station Reports (see table 1 below)
Table 1. Station reports (alphabetical by presenter).
Institution Presenter Title of Presentation
Michigan State University Evangelyn Alocilja Biosensor based on nano-assembly for rapid detection of pathogens
University of Illinois Kaustubh Bhalerao Bioinstrumentation for agricultural disease diagnostics
University of Wisconsin Sundaram Gunasekaran Visible Detection of Pathogens
University of Hawaii Daniel Jenkins Distributed Agricultural Diagnostics: An Engineering Evolution
Rutgers University Paul Takhistov Integrated Nano-Structured Sensor System for Category B Toxins Detection in Complex Biological and Environmental Matrices
Utah State University Anhong Zhou Gapped-duplex Approach to DNA Mismatch Detection

All station reports were completed by the evening of May 12, and participants unanimously approved concluding the official "business meeting" of NC-1194 at 6:46 PM.
7) At a working dinner, members discussed in detail ideas to further stimulate collaboration (see item 5 above), and tentatively recommended that the 2014 meeting be held in Honolulu, HI in February (based on empirical observations reported by Daniel Jenkins that airfares and hotels are generally more affordable in February).
8) By concluding the official business meeting on May 12, members were able to take more advantage of the opportunities to participate in activities at the Nanotechnology Expo. Members were especially encouraged to attend a biosensors technical session on May 15, at which several members reported their research.

Accomplishments

Accomplishments  Progress Reports: <br /> This is a progress report for the period October 1, 2012-September 30, 2013 by participating institutions covering various states. As a reminder, the objectives of this project are:<br /> 1. Develop new technologies for characterizing fundamental nanoscale processes<br /> 2. Construct and characterize self-assembled nanostructures<br /> 3. Develop devices and systems incorporating microfabrication and nanotechnology<br /> 4. Develop a framework for economic, environmental and health risk assessment for nanotechnologies applied to food, agriculture and biological systems<br /> 5. Produce education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment<br /> Progress reports are presented by state. The findings have been disseminated to the scientific community via seminars, national/international conferences, manuscripts, and web sites.<br /> Arizona (University of Arizona)<br /> Outputs<br /> 1) With the new collaborative research contract from QIA (Animal, Plant & Fisheries Quarantine & Inspection Agency, South Korea), a handheld polymerase chain reaction (PCR) device is currently being developed for rapid veterinary diagnostics. Three researchers from QIA have visited my lab in December 2012 to check our progress, and my research team (me and two of my graduate students) have visited QIA in June-July 2013 to deliver the alpha prototype and conducted experimental demonstrations. Discussion is currently made to commercialize this technology.<br /> 2) The same technology is being applied to detect blood infections. Two awards have been made in December 2012 (both projects have started in January 2013), one from AZ Furnace program and the other from Tech Launch Arizona, with the aim of commercializing this technology. A spin-off company, Fast PCR Diagnostics, LLC, has been created in December 2012. Negotiations are currently being made, including option agreement, exclusive practice right, conflict of interest, etc.<br /> Impacts<br /> 1) Wire-guided droplet PCR technology have been applied for veterinary diagnostics (influenza A) and blood infection, which is very fast and works with blood and tissue samples. A handheld prototype is currently being developed. A PCT application has been filed.<br /> 2) A novel method of creating ensemble nanotextured surfaces have been applied to not only endothelial cells, but also fibroblasts and smooth muscle cells. Controllable surfaces, using different types of polymer/nanofiber coatings and electrowetting technology, are also being developed towards creating better cardiovascular devices.<br /> 3) A simple paper microfluidics and smartphone-based optical detection is being considered for water quality monitoring (Cryptosporidium and endocrine-disrupting chemicals) and food safety application (Salmonella).<br /> Hawaii (University Hawaii)<br /> Some accomplishments from the University Hawaii were presented at the meeting by Dr. Daniel M. Jenkins (Table 1). A summary of these accomplishments and some others at the University is provided below, along with others completed in the lab of Dr Winston Su.<br /> Output. Work by Daniel Jenkins' group has focused largely on commercializing a new molecular probe technology for real-time, sequence-specific detection of isothermal nucleic acid amplification, along with a new low-cost, handheld instrument to run and analyze reactions in the field. In parallel, some efforts have focused on developing simple technologies to facilitate the rapid isolation and concentration of disperse biological agents and DNA from the environment to improve the sensitivity of diagnostics for agriculture and food safety. Specific activities/outputs include:<br /> 1) With collaborators from USDA-ARS in Riverside CA and Corvallis OR, we have adapted our probe technologies for the simple, rapid detection of the Candidatus Liberibacter asiaticus (citrus greening organism) directly in tissues of citrus psyllids (the insect vector of the disease), as well as for airborne spores of Erisyphe necator (powdery mildew) in orchards.<br /> 2) We have successfully developed and tested new assays for E. coli (species specific assays, and assays specific for O157 strains), and Clavibacter michiganensis ssp michiganensis (bacterial canker of tomato), to complement existing assays including a multiplexed typing assay for the bacterial wilt pathogen Ralstonia solanacearum, and the food borne pathogen Salmonella enteritidis.<br /> 3) We have refined the design of our handheld instrument to improve performance, and are currently engaged in a final redesign to include some primarily cosmetic improvements, with plans to have a prototype of a final commercial design available within several months.<br /> 4) We have developed and successfully tested a new handheld, non-instrumented incubator to enrich trace contaminations of E. coli to enable detection with our molecular platform. The new incubator is based on the same principle of Non-instrumented nucleic acid amplification devices reported in previous years.<br /> 5) Field evaluation of new technologies was conducted in coordination with the FDA mobile diagnostics lab at a field in Salinas CA, and more recently with a team of Medical Microbiologists with the US Navy in remote clinical laboratories in the Marshall Islands. System performance was generally quite good, except for some incidences of cross contamination between samples. As in results of field trials in a village in Guatemala in the previous year, these findings illustrate the need for ready-to-use kits and greater systems integration especially when used in rudimentary conditions in the field, and have largely informed our objectives for the upcoming year's work.<br /> 6) Several agreements related to IP and manufacturing are currently being concluded to begin selling diagnostic kits to commercial entities (ie. not just research use). Details of these agreements are still confidential for the company that has spun off from our work.<br /> 7) Results have been disseminated to scientific communities and to the public through a variety of publications, workshops, and especially technology transfer and cross evaluation with collaborators at the USDA, FDA, and Navy.<br /> During the same reporting period, the Su lab at the University of Hawaii continued to investigate biosynthesis and applications of protein nano-assemblies, with focus on two molecular platforms: one is based on protein-bound nano-oleosomes, and the other on self-assembled protein nanofibrils. Following the development of a novel method for facile biosynthesis of functional nano-scale oleosomes (discrete cellular organelles with a lipid core surrounded by a protein-embedded phospholipid monolayer) via the expression of oleosin-fusion proteins in oleaginous yeast Yarrowia lipolytica, engineered nano-oleosomes have been further developed with both cell-targeting and reporting activities, and their ability to target a specific cell type while bring along a unique catalytic function to the surface of the target cells has been demonstrated. With the nanofibril platform, additional studies have been conducted to examine synthesis of hybrid fibrils that display multiple protein and peptide ligands as supramolecular self-functionalized nanostructures using genetic fusion of the protein/peptide functional domains with a fibrillogenic domain from yeast.<br /> Impact. The technologies developed in this research are intended to allow rapid detection of agricultural and food-borne pathogens to improve food security and safety. Handheld rapid detection has been identified as a critical need by the USDA for enhancing food safety and implementing effective surveillance programs to exclude harmful pathogens from agricultural settings. <br /> In the 2011 annual project meeting for NC-1194 (then NC-1031), commercialization of sensor technologies was identified as being a critical activity as the technologies themselves are rapidly reaching maturity. We have already begun limited sales of molecular detection kits and custom handheld instruments for research use only, and are commercialization of these technologies for food safety and agricultural biosecurity.<br /> The work conducted in the Su lab has lead to facile synthesis of novel nano-biomaterials with tunable functionality. These materials offer new possibilities in biocatalysis, biosensing, and tissue engineering applications, and should help advancing food safety, agriculture biosecurity, and biomedical sciences.<br /> Illinois (University of Illinois Urbana-Champaign)<br /> Outputs<br /> The Bhalerao group continues to work on environmental impact on nanotechnology and has started a new collaboration with members of NC1194 on developing open source bioinstrumentation projects. In the AY 2012-13, two students obtained their MS degrees on the topic of environmental impact of nano tech. They showed that organisms can develop resistance to metal leachates of nanoparticles, and this phenomenon can be replicated by over expressing a single gene. Further the group has developed a novel image analysis platform to automate soil-dwelling pathogens, efforts are underway to commercialize this diagnostic. <br /> Impacts:<br /> The collaborative bioinstrumentation project will benefit researchers within NC1194 and beyond by developing a set of specifications, schematics and devices for interfacing biosensors with portable computers including modern smartphones. The soil pathogen detection and quantification system is currently being commercialized. <br /> Iowa (Iowa State University)<br /> OUTPUT The activities during this period included: 1. further development of multiplexing dual-recognition Raman sensing scheme for single step pathogen detection in a nanoDEP microfluidic platform, multiple epitope target detection is achieved at single cell level; 2. The development of nanophotocatalyst for food safety control. 3. Further development of Raman spectroscopic imaging-based characterization of glaucomatous retinal tissues. The Raman imaging technique may serve as the basis for a method to diagnose glaucoma at an early stage, which will greatly benefit glaucoma patients. 4. Further development of a THz spectroscopic detector and an ultrasonic imager for bacterial contamination inside eggs<br /> These findings were presented in several peer reviewed conferences and journal articles.<br /> OUTCOME/IMPACTS <br /> 1) The spectroscopic sensing techniques developed in this project are highly specific, they have the potential to meet the needs in various disciplines for high-accuracy target sensing, including early diagnosis of diseases (i.e., glaucoma), and rapid screening for foodborne pathogens. <br /> 2) The development of a nano-enabled antimicrobial biodegradable coating for food safety control utilizes biodegradable films made from soy and corn proteins, and functionalizes them with antimicrobial nanoparticles to improve their mechanical strength and to impart in the film antimicrobial functionality. Cheap coating materials can be made that are antimicrobial and can be used in food processing/food handling places to improve food safety, such as fresh produce and meat processing facilities. <br /> 3) The THz spectroscopic and ultrasonic screening of internal bacterial contamination of eggs potentially can significantly reduce contamination-induced losses to poultry husbandry.<br /> Michigan (Michigan State University)<br /> Accomplishments from Michigan State University were presented at the meeting by Dr. Evangelyn Alocilja (Table 1). For details, please refer to http://www.egr.msu.edu/~alocilja/. A written report is also presented below.<br /> Output. Research accomplishments at Michigan State University include the development of a hybrid nanomaterial in the form of magnetic-gold nanoparticles as well as the development of a bio-inspired nanoparticle-based biosensor. We have validated our biosensor in various food matrices (spinach, milk, and apple juice). We have also improved our pathogen extraction techniques. In addition, we have initiated a research program for the development of rapid detection of tuberculosis both in humans and animals.<br /> Impact. Dr. Alociljas technologies were featured in the Science of Innovation educational program by the National Science Foundation and the US Patent Office through the NBC Learn as a national resource to encourage and recruit K-12 students to the science fields. The video is entitled Science of Innovation: Anti-Counterfeiting Devices and can be viewed at www.nbclearn.com/innovation/cuecard/62970. This material will impact thousands of K-12 students and teachers not only in the US but also around the world. Dr. Alocilja was also an invited speaker in various professional meetings, allowing the dissemination of her research work to a broader group of researchers and potential users. Furthermore, technology transfer was a continuing activity for Dr. Alocilja as her technologies are being licensed for commercialization by a start-up company, leading to the creation of jobs in Michigan.<br /> Missouri (University of Missouri)<br /> Output: Research accomplishments at University of Missouri include: 1) Development and use of gold nanoprobes coupled with superparamagnetic beads for rapid detection of aflatoxin M1 in milk by dynamic light scattering; 2) Facile synthesis of Au-Ag core-shell nanoparticles with uniform sub-2.5 nm interior nanogaps for surface-enhanced Raman scattering (SERS) applications; 3) Detection of herbicides in drinking water by SERS coupled with gold nanostructures. We have developed a variety of novel nanostructures using gold, silver, and other materials and studied their potential applications in food safety, specifically for rapid detection of chemical and biological contaminants in food matrices. <br /> We have disseminated the results to the industry and scientific communities at professional conferences such as IFT, ACS, and IAFP. <br /> Impact: This study will help improve public health by quickly detecting biological contaminants in foods and consumers will benefit from improved food safety; Having sensitive and rapid analytical methods would assist regulatory agencies and the food industry to better assess product safety early on and increase public confidence in our food supply.<br /> New Jersey (Rutgers University)<br /> During reporting period the Paul Takhistov group at Rutgers focused on methods to improve sensitivity and selectivity of biosensors utilizing nano-patterned surfaces.<br /> Output: We have developed method to Immobilize biorecognition molecules via self-assembled monolayer on metal oxide nano-structured surface. A method of antibody (Ab) immobilization on a nanoporous aluminum surface for an electrochemical immunosensor is presented. To achieve good attachment and stability of Ab on an aluminum surface, aluminum was silanized with 3-aminopropyltryethoxysilane (APTES), and then covalently cross-linked to self-assembled layers (SALs) of APTES. Both the APTES concentration and the silanization time affected the formation of APTES-SALs as Ab immobilization. The formation of APTES-SALs was confirmed using the water contact angle on the APTES-SALs surface. The reactivity of APTES-SALs with Ab was investigated by measuring the fluorescence intensity of fluorescein isothiocyanate-labeled Ab-immobilized on the aluminum surface. Silanization of aluminum in 2% APTES for 4 h resulted in higher water contact angles and greater amounts of immobilized Ab than other APTES concentrations or silanization times. More Ab was immobilized on the nanoporous surface than on a planar aluminum surface. Electrochemical immunosensors developed on the nanoporous aluminum via the Ab immobilization method established in this study responded functionally to the antigen concentration in the diagnostic solution.<br /> Impacts: Methods for Ab immobilization established in this study are valid for development of an electrochemical immunosensor based on a nanoporous aluminum surface.<br /> New York (Cornell University)<br /> Efforts have focused on the novel approach of using electrospun nanofibers in microfluidic and lateral flow assay biosensor platforms. Here, studies have looked into using varying polymer materials to function as separation and biodetection modules. Previous work had successfully demonstrated the ability to isolate negatively charged nanovesicles via positively charged polyvinyl alcohol nanofibers out of solution; and selectively discharge them again from the nanofiber mats. Current research focused on using de novo nanofiber mats made from poly lactic acid doped with a variety of functional polymers. Here successful synthesis of nanofibers, fabrication of a lateral flow assay platform and functional analysis were performed. Direct binding assays using antibodies as biorecognition elements were established and a successful sandwich assay for the detection of pathogenic E. coli O157 was demonstrated. The research is summarized in a manuscript submitted to Analytical and Bioanalytical Chemistry and is currently under review. <br /> Future research will focus on further innovative studies of nanofibers in microfluidic and lateral-flow assay biosensor platforms with special emphasis of realizing technologies that are capable of detecting analytes in complex samples. <br /> South Carolina (Clemson University)<br /> The research team at Clemson University, under Dr. Jeremy Tzeng, has participated in Objective 3 of the proposed project developing nanoscale devices and systems. Their tasks are to develop alternative microbial capturing agents for use in biosensor applications, and to develop microfluidic devices for manipulation of bacterial particle movement. They have evaluated adhesin-specific nanoparticals, including gold, silver, and iron-oxide as well as GREEN nanoparticles, for their bindings to specific microorganisms and for their cytotoxicity to mammalian cells. We have also fabricated AC/DC driven microfluidic devices for focusing, concentrating, trapping, followed by an in-line RF sensor for detection, enumeration, and differentiation of viable and non-viable targets in low number. Seven manuscripts and several conference proceedings have been published during this project year.<br /> Utah (Utah State University)<br /> Outputs<br /> Research activities in this project include: 1) designed and fabricated prototype multiple sensor arrays that can be used for multiple DNA detection, the sensor array performance has been initially evaluated; 2) different versions of microfluidic device have been designed and fabricated; 3) a label free approach has been validated to measure the different mismatched DNA sequences immobilized on individual gold sensor surface; 4) a few ruthenium (II) complex compounds have been tested for DNA binding affinity. Based on the binding affinity, these compounds could be used as probe for DNA detection.<br /> Also, the research results were presented to local community through various on campus outreach programs: Biotechnology Summer Academy, Engineering State, and Biological Engineering Summer Internship, etc PIs laboratory has been hosted several high school students annually through these active outreach programs.<br /> Outcomes/Impacts<br /> The U.S. Environmental Protection Agency estimates that 155 million people in the United States are at risk of exposure to Cryptosporidium. According to the report from the Bear River Health Department (BRHD), North Logan, Utah, a total of 129 cases were reported in the BRHD District in 2007. Considering these national and local concerns, species-specific rapid detection and identification to various genotypes of Cryptosporidium could be significant to provide a tool to determine the source of the pathogen in a human epidemic and further identification of the source of an outbreak, by distinguishing the DNA sequences that are specific to different species of Cryptosporidium. The ultimate goal of this five-year AES project is to develop a biochip device (or called Lab-on-a-chip) or biosensor instrument that is capable of detecting various genotypes of waterborne pathogen Cryptosporidium DNA, which could help the determination of outbreak sources of this waterborne pathogen, water surveillance monitoring, as well as be beneficial to food safety and security for local and national food industry. <br /> While we are actively working on the bench lab research on the performance evaluation and validation of multiple DNA detection through this proposed lab-on-a-chip sensor device, we also have been actively working with universitys commercialization office for the technology protection. Several provisional patents have been filed for related sensor and device design and fabrication.<br /> Wisconsin (University of Wisconsin)<br /> Some accomplishments from the Wisconsin were presented at the meeting by Dr. Sundaram Gunasekaran (Table 1). A summary of these accomplishments is provided below.<br /> Output. Work by Gunasekarans group is on visible detection of pathogen and other agents to ensure food safety. Specific activities/outputs include:<br /> 1) The development of a nanobiosensor, which gives raise to a color change in the presence/absence of pathogenic bacteria. It is an immunosensor. However, a red-to-purple color change occurs when a bifunctional linker, that is not attached to the target pathogen, binds the gold nanoparticles.<br /> 2) In situ synthesis of gold nanoparticles as novel time-temperature indicator for food quality monitoring. We have been synthesizing gold nanoparticles from hydrogen tetrachloroaurate, a gold precursior, in the presence of gelatin, a food protein, which both acts as a reluctant and a stabilizer. Using this nanoreactor system we have been developing a novel nanomaterial-based thermal history indicator (nTHI).The color of nTHIs begins to change and continues to become more intense with storage time; providing an indirect colorimetric indication of food quality as it is affected by storage time and temperature suitablefor real-time, continual monitoring of thermal history of foods to help track temperature abuse and ensure food safety during post-harvest handling, transportation, and storage. <br /> 3) Electrochemical biosensors to detect toxins in complex food matrices. We have been synthesizing various nanostructures (graphene, multi-walled carbon nanotubes etc.). By employing enzymatic, non-enzymatic, and immunosensing approaches, we are pursuing detection of various analytes including glucose, dopamine, hydrogen peroxide, and toxins. <br /> Impact. These systems have proven to be highly sensitive. They could potentially be used for on-site detection even at the consumer level. <br /> Work plan for the next year:<br /> Research by each investigator at each participating institution will continue as funding becomes available. New techniques will be developed for characterizing fundamental nanoscale processes. Self-assembled nanostructures will be designed and characterized. New biosensor devices and systems will be developed and will continue to incorporate microfabrication and nanotechnology. Economic frameworks and environmental and health risk assessments of nanomaterials as applied to food, agriculture and biological systems will continue to be a priority. And finally, educational and outreach materials on nanofabrication, sensing, systems integration and application risk assessment will be developed.<br />

Publications

Peer Reviewed Journals:<br /> <br /> Anderson, M.J., Miller, H.R., and Alocilja, E.C. 2013. PCR-less DNA co-polymerization detection of Shiga like toxin 1 (stx1) in Escherichia coli O157:H7. Biosensors and Bioelectronics, 42, 581-585. <br /> <br /> Angus, S.V., Kwon, H.-J., Yoon, J.-Y. 2012. Field-Deployable and Near-Real-Time Optical Microfluidic Biosensors for Single-Oocyst-Level Detection of Cryptosporidium parvum from Field Water Samples. Journal of Environmental Monitoring, 14(12): 3295-3304.<br /> <br /> D.C. Canaday, J.L. Salak-Johnson, A.M. Visconti, X. Wang, K. Bhalerao and R.V. Knox (2013), Effect of variability in lighting and temperature environments for mature gilts housed in gestation crates on measures of reproduction and animal well-being. Journal of Animal Science 91 (3) 1225-36.<br /> C. Chai, J. Lee, J. Park, P. Takhistov (2012) Antibody immobilization on a nanoporous aluminum surface for immunosensor development, Applied Surface Science, http://dx.doi.org/10.1016/j.apsusc.2012.09.027<br /> <br /> Changhoon Chai, Paul Takhistov (2012) Control of the lateral interactions of immobilized proteins using surface nanoporous-patterning , Applied Surface Science, http://dx.doi.org/10.1016/j.apsusc.2012.09.008<br /> <br /> Fronczek, C.F., You, D.J., Yoon, J.-Y. 2013. Single-Pipetting Microfluidic Assay Device for Rapid Detection of Salmonella from Poultry Package. Biosensors and Bioelectronics, 40(1): 342-349.<br /> Gajaraj S.; Fan, C.; Lin, M.; Hu, Z. 2013. Quantitative detection of nitrate in water and wastewater by surface-enhanced Raman spectroscopy. Environ. Monit. Assess. 185 (7), 5673-5681. <br /> Gamboa, J.R., Mohandes, S., Tran, P.L., Slepian, M.J., Yoon, J.-Y. 2013. Linear Fibroblast Alignment on Sinusoidal Wave Micropatterns. Colloids and Surfaces B: Biointerfaces, 104: 318-325.<br /> Han, Z., Madzak, C., Su, W.W. 2013. Tunable nano-oleosomes derived from engineered Yarrowia lipolytica. Biotechnol. Bioeng. 110(3), 702-710. <br /> Kadam, D. M., M. Thunga, S. Wang, M. R. Kessler, D. Grewell, B. Lamsal, Chenxu Yu, Preparation and Characterization of Whey Protein Isolate Films Reinforced with Porous Silica Coated Titania Nanoparticles, Journal of Food Engineering, 117, 133-140, 2013.<br /> Kubota, R., P. LaBarre, B. H. Weigl, and D. M. Jenkins. 2013. Molecular diagnostics in a teacup: non-instrumented nucleic acid amplification (NINA) for rapid, low cost detection of Salmonella enterica. Chinese Science Bulletin. 58(1):1-7.<br /> Liang, P.-S., Yoon, J.-Y. 2013. Optofluidic Lab-on-a-chip Monitoring of Subsurface Bacterial Transport, Biological Engineering Transactions. 6(1): 17-28. Featured in ASABE Publications.<br /> Lim S, S Gunasekaran, J-Y Imm. 2012. Gelatin-templated gold nanoparticles as novel time-temperature indicator. J Food Science 77(9):N45-49.<br /> Liu, B., Zhou, P.*, Liu, X.M., Sun, X., Li, H., Lin, M. 2013. Detection of pesticides in fruits by surface-enhanced Raman spectroscopy coupled with gold nanostructures. Food and Bioprocess Technology 6(3), 710-718.<br /> Marrero, G., K. L. Schneider, D. M. Jenkins, and A. M. Alvarez. 2013. Phylogeny and classification of Dickeya based on multilocus sequence analysis. International Journal of Systematic and Evolutionary Microbiology. (In Press).<br /> McCracken, K.E., Tran, P.L., You, D.J., Slepian, M.J., Yoon, J.-Y. 2013. Shear- vs. Nanotopography-Guided Control of Growth of Endothelial Cells on RGD-Nanoparticle-Nanowell Arrays. Journal of Biological Engineering, 7: 11.<br /> McGraw, S., Alocilja, E.C.*, Senecal, A.G., and Senecal, K.J. 2012. Synthesis of a Functionalized Polypyrrole Coated Electrotextile for Use in Biosensors. Biosensors, 2(4), 465-478. <br /> McGraw, S., Alocilja, E.C., Senecal, K.J., and Senecal, A.G. 2012. A Resistance Based Biosensor That Utilizes Conductive Microfibers for Microbial Pathogen Detection. Open Journal of applied Biosensors, 1, 36-43.<br /> Packard, M.M., Shusteff, M., and Alocilja, E.C. 2012. Sample Preparation by On-Chip Dielectrophoretic Separation and Concentration of Viable, Non-Viable and Viable but Not Culturable (VBNC) Escherichia coli. In Proceedings of NSTI Nanotech 2012: Bio Sensors, Instruments, Medical, Environment and Energy, 3, 21-24. ISBN 978-1-4665-6276-9 .<br /> Packard, M.M., Shusteff, M. and Alocilja, E.C. 2012. Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH). Biosensors, 2(4), 405-416. <br /> Packard, M.M., Wheeler, E.K., Alocilja, E.C., and Shusteff, M. 2013. Performance evaluation of fast microfluidic thermal lysis of bacteria for diagnostic sample preparation. Diagnostics, 3(1), 105-116. <br /> Patel, S., D. Showers, P. Vedantam, T.-R. Tzeng, S. Qian and X. Xuan (2012). "Continuous-flow separation of live and dead yeasts using reservoir-based dielectrophoresis (rDEP)." Bulletin of the American Physical Society 57.<br /> Patel, S., D. Showers, P. Vedantam, T.-R. Tzeng, S. Qian and X. Xuan (2012). "Microfluidic separation of live and dead yeast cells using reservoir-based dielectrophoresis." Biomicrofluidics 6: 034102.<br /> P. Poisson and K. Bhalerao (2013) Hidden hysteresis  population dynamics obscures gene network dynamics. Journal of Biological Engineering Vol. 7, No. 16. <br /> Song, X.; Li, R.; Li, H.; Hu, Z.Q.; Mustapha, A.; Lin, M. 2013. Characterization and quantification of zinc oxide and titanium dioxide nanoparticles in foods. Food and Bioprocess Technology. In press.<br /> Song, X.; Li, H.; Al-Qadiri, H.M.; Lin, M. 2013. Detection of herbicides in drinking water by surface-enhanced Raman spectroscopy coupled with gold nanostructures. Journal of Food Measurement & Characterization. In press.<br /> Stemple, C.C., Angus, S.V., Park, T.S., Yoon, J.-Y. 2013. Smartphone-Based Optofluidic Lab-on-a-Chip for Detecting Pathogens from Blood, JALA - Journal of Laboratory Automation, (In Press).<br /> A. Stirling, J.D. Tabara, K. Bhalerao and S. Pauwels (2012), Risk Assessment and Sustainability of Synthetic Biology for Agriculture. International Journal of Social Ecology and Sustainable Development. Invited article (with honorarium) for a special issue on Synthetic Biology.<br /> Su, W.W. and Han, Z. 2013. Self-assembled synthetic protein scaffolds: biosynthesis and applications. ECS Trans. 50(28): 23-29.<br /> Subroy, V., D. Gimenez, P. Takhistov. (2012). "On Determining Aggregate Bulk Density by Displacement in Two Immiscible Liquids." Soil Sci. Soc. Am. J. 76(4): 1212-1216<br /> M. J. Tang, G. D. McEwen, Y. Wu, C. D. Miller, A. Zhou, "Characterization and analysis of Gram-positive and Gram-negative bacteria and their co-culture mixtures by Raman microspectroscopy, FT-IR, and atomic force microscopy", Analytical and Bioanalytical Chemistry, 2013, 405, 15771591.<br /> Tran, P.L., Gamboa, J.R., McCracken, K.E., Riley, M.R., Slepian, M.J., Yoon, J.-Y. 2013. Nanowell-Trapped Charged Ligand-Bearing Nanoparticle Surfaces - A Novel Method of Enhancing Flow-Resistant Cell Adhesion. Advanced Healthcare Materials, 2(7): 1019-1027. Back Cover.<br /> Vedantam, P., T.-R. J. Tzeng, A. K. Brown, R. Podila, A. Rao and K. Staley (2012). "Binding of Escherichia coli to functionalized gold nanoparticles." Plasmonics 7(2): 301-308.<br /> Wang, F., Y. Raval, H. Chen, T. R. J. Tzeng, J. D. DesJardins and J. N. Anker (2013). "Development of Luminescent pH Sensor Films for Monitoring Bacterial Growth Through Tissue." Advanced healthcare materials.<br /> Wang, C., Chenxu Yu, Detection of chemical pollutant in water using gold nanoparticles as sensors: a review, Reviews in Analytical Chemistry, 32(1), 1-14, 2013.<br /> Wang, Q., S. Grozdanic, M. H. Harper, K. Hamouche, N. Hamouche, H. Kecova, T. Lazic and C. Yu, Detection and characterization of glaucoma-like canine retinal tissues using Raman spectroscopy, Journal of Biomedical Optics, 18(6), 067008, 2013.<br /> Wang, C., C. G. Yoo, Chenxu Yu, T. Kim, Raman Spectroscopic characterization of Photonanocatalyst Aided Alkaline Pretreated Corn Stover biomass, Advanced Materials Research, (In Press).<br /> Wang Y-C, S Gunasekaran. 2012. Spectroscopic and microscopic investigation of gold nanoparticle nucleation and growth mechanisms using gelatin as a stabilizer. J Nanoparticle Research 14(10):1200.<br /> Wang, Y., and Alocilja, E.C. 2012. Sensor Technologies for Anticounterfeiting. International Journal of Comparative and Applied Criminal Justice, 36(4), 291-304.<br /> Wu, H. T., D. M. Li, B. W. Zhu, J. H. Cheng, J. J. Sun, F. L. Wang, Y. Yang, Y. K. Song, and C. Yu, Purification and characterization of alkaline phosphatase from the gut of sea cucumber Stichopus japonicas, Fisheries Science, 79(3), 477-485, 2013.<br /> Xuan, P., Y. Zhang, T.-r. J. Tzeng, X.-F. Wan and F. Luo (2012). "A quantitative structureactivity relationship (QSAR) study on glycan array data to determine the specificities of glycan-binding proteins." Glycobiology 22(4): 552-560.<br /> Yang J, JR Strickler, S Gunasekaran. 2012. Indium tin oxide-coated glass modified with reduced graphene oxide sheets and gold nanoparticles as disposable working electrodes for dopamine sensing in meat samples. Nanoscale 4(15): 4594-4602.<br /> Yang, J., B. Zhu, J. Zhang, L. Sun, D. Zhou, X. Ding, and C. Yu, Stimulation of lymphocyte proliferation by oyster glycogen sulfated at C-6 position. Carbohydrate Polymers, 94, 301-308, 2013.<br /> Yasuhara-Bell, J., R. Kubota, D. M. Jenkins, and A. Alvarez. Loop-mediated amplification of the Clavibacter michiganensis subsp. michiganensis micA gene is highly specific. Phytopathology (In Press).<br /> Yoo, C. G., C. Wang, C. Yu, T. H. Kim, Enhancement of enzymatic hydrolysis and lignin removal of corn stover using photocatalyst-assisted ammonia pretreatment, Applied Biochemistry and Biotechnology, 169, 1648-1658, 2013.<br /> You, D.J., Park, T.S., Yoon, J.-Y. 2013. Cell-Phone-Based Measurement of TSH Using Mie Scatter Optimized Lateral Flow Assays. Biosensors and Bioelectronics, 40(1): 180-185.<br /> Zeng, J., C. Chen, P. Vedantam, V. Brown, T.-R. J. Tzeng and X. Xuan (2012). "Three-dimensional magnetic focusing of particles and cells in ferrofluid flow through a straight microchannel." Journal of Micromechanics and Microengineering 22(10): 105018.<br /> Zeng, J., Y. Deng, P. Vedantam, T.-R. Tzeng and X. Xuan (2013). "Magnetic separation of particles and cells in ferrofluid flow through a straight microchannel using two offset magnets." Journal of Magnetism and Magnetic Materials.<br /> Zhang, Z.; Lin, M.; Zhang, S.; Vardhanabhuti, B. 2013. Detection of aflatoxin M1 in milk by dynamic light scattering coupled with superparamagnetic beads and gold nanoprobes. J. Agric. Food Chem. 61 (19), pp 45204525.<br /> Zhang, Z.; Kong, F.; Vardhanabhuti, B.; Mustapha, A.; Lin, M. 2012. Detection of engineered silver nanoparticle contamination in pears. J. Agric. Food Chem. 60(43), 1076210767.<br /> Zhang, Z.; Zhang, S.; Lin, M. 2013. Facile synthesis of Au-Ag core-shell nanoparticles with uniform sub-2.5 nm interior nanogaps. Chemical Communications. In press.<br /> <br /> <br /> Conference Proceedings (Peer-Reviewed)<br /> Park, T.S., Harshman, D.K., Fronczek, C.F., Yoon, J.-Y. 2013. Smartphone Detection of Escherichia coli from Wastewater Utilizing Paper Microfluidics. The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), Freiburg, Germany, 27-31 October 2013, accepted (oral).<br /> Fronczek, C.F., Park, T.S., Yoon, J.-Y. 2013. Paper Microfluidic Extraction of Bacterial and Viral Nucleic Acid from Field and Clinical Samples towards a Direct MicroTAS Apparatus. The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), Freiburg, Germany, 27-31 October 2013, accepted (poster).<br /> Harshman, D.K., Reyes, R., Yoon, J.-Y. 2013. Direct Detection of Plasmid-Mediated Antibiotic Resistance in Bloodstream Infection by PCR Using Wire-Guided Droplet Manipulation (WDM). The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013), Freiburg, Germany, 27-31 October 2013, accepted (poster).<br /> <br /> Conference Abstracts<br /> Harshmann, D.K., Reyes, R., You, D.J., Yoon, J.-Y. 2012. Device for Near-Instant Diagnosis of Clinical Infection by Convective Droplet Thermocycling and 16s rRNA Hybervariable Region Probes. BMES 2012 Annual Meeting, Atlanta, GA, 24-27 October, 2012.<br /> Park, T.S., Li, W., McCracken, K.E., Yoon, J.-Y. 2013. Paper Microfluidics Detection of Salmonella Using a Smartphone. 2013 Annual Meeting of IBE, Raleigh, NC, 7-9 March 2013.<br /> Harshman, D.K., Reyes, R., Park, T.S., You, D.J., Yoon, J.-Y. 2013. Extremely Fast Nucleic Acid Amplification by Droplet Manipulation for Point-of-Care Diagnosis of Blood Infection. 2013 Annual Meeting of IBE, Raleigh, NC, 7-9 March 2013.<br /> Liang, P-S., Yoon, J.-Y. 2013. Rapid Detection of Foodborne Pathogens within Meat Utilizing a Smartphone Biosensor. 2013 ASABE Annual International Meeting, Kansas City, MO, 21-24 July 2013.<br /> Liang, P.-S., Yoon, J.-Y. 2013. Using Biosensor in Secondary Education Curriculum to Improve Students Interest and Awareness of Science, Engineering, and Current Worldwide Issues. 2013 ASABE Annual International Meeting, Kansas City, MO, 21-24 July 2013.<br /> Park, T.S., Li, W., Liang, P.-S., Yoon, J.-Y. 2013. Paper Microfluidics Detection of Salmonella Using a Smartphone. 2013 ASABE Annual International Meeting, Kansas City, MO, 21-24 July 2013.<br /> Yu, C., Q. Wang,* E. Campos,*K. Hamouche. Evaluating meat quality using Raman spectroscopy. 3rd World Congress of Agriculture, Hangzhou, China, (September 23-25, 2013). <br /> Yu, C., Raman biosensors for multiplex screening of food pathogens, Emerging Technologies Symposium, International Association for Food Protection (IAFP), Charlotte, NC, (July 30, 2013)<br /> Wang, C., C. Yu, F. R. Madiyar, J. Li, Nano-dielectrophoresis chip integrated with Raman spectroscopic self-referencing detection of foodborne pathogens. 3rd International Conference on Bio-sensing Technology, Sitges, Spain (May 12-15, 2013)<br /> <br /> Patents:<br /> Gunasekaran S, Lim S. 2012. Nanoreactors as Time-Temperature Indicators (US Patent Provisional Application No. 61/374,127).<br /> <br /> <br /> <br /> Participants<br /> 1. Individuals: Jeong-Yeol Yoon (PI); Tu San Park (post-doc); Jessica R. Gamboa, Pei-Shih Liang, Christopher F. Fronczek, Dustin K. Harshman, Scott V. Angus, Cayla Baynes, Ariana M. Nicolini, Wenyue Li (graduate students); Katherine E. McCracken, Wenyue Li, Samir Mohandes, Roberto Reyes, Lily Walsh, Ariana Nicolini, Scott Brechbiel, Michael Bollig, Erin Cahill, Soohee Cho (undergraduate students).<br /> 2. Partner organization: Animal, Plant & Fisheries Quarantine & Inspection Agency (QIA), South Korea.<br /> 3. Collaborators: Jae-Young Song (QIA), Marvin J. Slepian (University of Arizona College of Medicine).<br /> 4. Chenxu Yu, Assistant Professor, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 5. Tong Wang, Professor, Iowa State University, Department of Food Science and Human Nutrition<br /> 6. David Grewell, Associate professor, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 7. Buddhi Lamsal, Assistant Professor, Iowa State University, Department of Food Science and Human Nutrition<br /> 8. Chien-Ping Chiou, Associate Scientist, Center for non-destructive evaluation<br /> 9. Qi Wang, Graduate Student, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 10. Chao Wang, Graduate student, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 11. Karl Hamouche, undergraduate student, Iowa State University, Department of Biomedical sciences<br /> 12. Shaowei Ding, undergraduate student, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 13. Muhua Liu, visiting scholar, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 14. Dattatreya Kadam, postdoc, Iowa State University, Department of Agricultural and Biosystems Engineering<br /> 15. Linxing Yao, postdoc, Iowa State University, Department of Food Science and Human Nutrition<br />

Impact Statements

Back to top

Report Information

Participants

Brief Summary of Minutes

Minutes attached here; annual report attached below, as the Publications document.

Accomplishments

Publications

NC1194's annual report attached below as Publications attachement.

Impact Statements

Back to top

Report Information

Participants

Brief Summary of Minutes

Please see attached file for NC1194's 2015/2016 annual report.

Accomplishments

Publications

Impact Statements

Back to top