Brief Summary of Minutes of Annual Meeting

In 2005, 24,533 seed lots/clones from 17,027 accessions were distributed: 15,067 (61%) USA requests and 9466 (39%) were distributions to foreign countries. There were 824 orders filled made up of 602 different requestors. The main crop requested was grass (5486) then safflower (3381) then alfalfa, beans, peas and chickpea all around 2000-2500 packets per genera. 49,229 observation data points were entered in GRIN in 2005 on 10,029 accessions on 192 descriptors on 22 crops. Thirty-nine per cent of the data came from cooperators and the other sixty-one per cent came from personnel at our station. 2477 viability records were entered on 2360 inventories. 1424 were tested in Pullman and 981 came from NSSL and the rest came from other sources. Of these 2477 fifty-five per cent were considered backlog inventories meaning they were over three years old. 1742 inventories were sent to NCGRP for backup. 2554 inventories were package and stored from newly increased/harvested accessions. 7562 inventories were weighed and seed number calculated. 2005 Germplasm Regeneration Activities: Agronomy Program, 710 accessions; Bean Program, 441 accessions; Cool Season Food Legume Program, 990 accessions; Horticultural Crops Program, 738 plots (308 were garlic); Forage Legume Program, 420 accessions, 92 Lotus, 173, Medicago and 155 Trifolium; Arid Land Plant Program, 2,247 accessions (77.8% for other NPGS, 22.2% Parlier's priority) for a total of 5,546 accessions regenerated for distribution and back up at our secondary site (Fort Coolins, CO). 2005 Germplasm Descriptor Data Generation- all the data is available on GRIN: Agronomy Program, 1,221 data points (46% came from cooperators); Bean Program, 15,335 data points (6% came from cooperators); Cool Season Food Legume Program,13,495 (52% came from cooperators), Horticultural Crops Program, 12,281 (99.9% came from cooperators); Forage Legume Program, photographed 350 accessions to document flowers and seeds, entered Lotus passport data into GRIN; Arid Land Plant Program: 97 Lesquerella and Limnanthes accessions were characterized for morphological and phenological characteristics and data were entered to GRIN. AGRONOMIC, GRASS AND SAFFLOWER project, Curator Vicki Bradley. 3,500 data points were entered in in 2005. Descriptor data, including fresh-flower-color, dry-flower-color, maximum and minimum head diameter, maximum and minimum height, spines, and bloom date, were collected on 74 safflower accessions for entry into GRIN. The number of overwintered plants and rust ratings were collected on 472 grasses. We began a project to update passport data on grass accessions, beginning with Achnatherum hymenoides (Indian Rice grass). Curator Vicki Bradley attended the Kansas Black Farmers Association (KBFA) tef meetings to participate in a review of 2005 accomplishments. Data gathered on 125 WRPIS accessions evaluated by a KSU graduate student was presented to the group and a demonstration plot planted on one of the KBFA member's farm was visited. These KBFA tef plots were the topic of an Associated Press article early in 2006. Recently, the Ethiopian government banned the export of tef flour making it probable that this work will be more valuable to the U.S. Ethiopian community than originally anticipated. We continued a study to identify duplicate accessions within our grass germplasm collection. Second year field data was collected on two populations of Dactylis glomerata 4Latar3, and four populations of Bromus inermis 4Manchar', planted at Central Ferry, Washington in a replicated evaluation plot in the fall of 2003. We used AFLP analysis on the DNA extracted from the Bromus populations and results indicated there were no differences between them. Comparison of the field data with the molecular data is underway. We have also started plants of other Bromus inermis accessions and will do similar analyses on these. HORTICULTURE CROPS, Curator Barbara Hellier. In 2005 the Horticulture Crops Program germplasm conservation efforts focused on increase/maintenance, documentation, and acquisition. Our increase program was spread across 16 locations. Greenhouse increases included accessions of Beta, Allium, Lactuca, Callistephus, Scabiosa, Plantago, and Polygonum. For the Beta accessions we finished harvest on 12 accessions, partially harvested 19 and started 31. In the Pullman fields we had a total of 518 plots: 308 garlic/ Allium and 210 broadleaf accessions. We continued to harvest the established broadleaf and Allium nurseries. Hightlights for 2005 include participated in several germplasm collection efforts. The first was in conjuction with our project to assess the genetic diversity of Allium acuminatum in the Great Basin. We collected A. acuminatum bulbs in northeast Nevada from 22 populations. The second was to locate and assess phenology of A. cernuum and Heirochloe odorata in northwestern Montana. The last trip was an NPGS sponsored collection mission to Greece to collect Beta nana. This was a multinational effort. The team consisted of four scientists: 2 from the US, 1 from Greece, and 1 from Germany. Genetic Diversity Patterns of Allium acuminatum in the Great Basin: Collaborative project with Dr Richard Johnson. Evaluation of the National Plant Germplasm System Garlic Collection for Garlic Viruses: NPGS funded evaluation. Collaborative project with Dr. Hanu Papu and Dr. Frank Dugan. Cool Season Food Legume Project, Curator Dr. Clarice Coyne, completed collection of 173,872 plant descriptor data from replicated plots of the pea core collection was planted in two locations (2685 plots) in two years (2004-05). For molecular characterization, approximately 6,600 SSR alleles and 16,800 RAPD alleles were generated on the pea core collection. Conducted replicated field trail in two cooperator locations for QTL mapping of quantitative resistance to Aphanomyces root rot and Fusarium root rot and presented data at four meetings to peers and one to the U.S. grain legume industry. Cooperated on germplasm evaluations of pea and chickpea for Fusarium root rot and Ascochyta blight. Conducted analysis to determine seed protein concentration data on 480 accessions of the pea collection and 40 accessions of chickpea core collection. Conducted pea BAC library characterization and utilization experiments in collaboration with visiting scientists. Mentored student awarded graduate research fellowship to study perennial Cicer collection. Conducted USDA-ARS Postdoctoral Fellowship project on haplotyping for association mapping studies in the cool season food legume collections. Phaseolus project. Curator Dr. Molly Welsh improved regeneration production: 43% increase in accessions regenerated due to the return to production of all of the greenhouse space-after renovation of irrigation system. Ninety-four accessions of species other than Phaseolus vulgaris were increased in 2005, representing a 200% gain from the previous year. Included is the increase of 17 wild accessions representing a 54% improvement over year 2004. All accessions grown were tested for the presence of BCMV (bean common mosaic virus). Seventy nine accessions have been through the virus clean-up program this year, representing a 97.5% gain. Information contained in the GRIN web-page descriptor site has been updated, and continues to be monitored and changed as additional data is obtained. Emphasis is placed on updating the information to keep it a current resource. The program to update and clarify the information in GRIN successfully entered data not previously available in the system for 1230 accessions. Two hundred sixty seven accessions-representing eight species & eleven spp., not previously deposited there, were sent to the NSSL for back-up storage; three species were represented in the seed sent for long-term storage. Forage Legume Prosser Highlights, Curator Dr. Stephanie Greene reported the uploading the upgraded Lotus passport data into GRIN, as well as made numerous corrections to GRIN to improve documentation. All G numbers have had extensive documentation added to GRIN. We uploaded passport data for explorations carried out in Georgia, and the Western US in 2004 (for all species collected). Have uploaded evaluation data generated on the M. truncatula collection in a 2003 morphological evaluation, and an aluminum tolerance evaluation carried out by the Noble Foundation. We are now largely independent in our use of the new GRINWIN system; routinely uploading our own annual regeneration and new acquisition data into GRIN. Photographed more than 350 accessions to document flowers and seeds. Photos have scale bar and color standards and are set up to allow for future image analysis of seed and flower color, shape and size. Photos will also be uploaded into GRIN. Arid Land Plant Program Parlier Highlights, Curator Dr. Maria Jenderek : Evaluation of orchardgrass. Cooperative project with USDA, ARS Pullman (R.C. Johnson) and Madison, WI (M. Casler). Breeding non-flowering orchardgrass. First year of observations is completed. The project will be carried out in 2006. Genetic diversity of the NPGS Limnanthes germplasm collection measured by SSR markers. This project was carried out in cooperation with CSUF, and was completed in 2005. The first manuscript draft is written. The results will depict the level of the collection's diversity. Variation in seed production and phonological characteristics in selected accessions of the USDA Limnanthes germplasm collection. Several traits of 24 different L. alba accessions were evaluated in a replicated trial. The 2nd year evaluation was completed. The project will be continued in the year. The study will provide information to breeders and growers of meadowfoam. Evaluation of the USDA Opuntia sp. germplasm collection. Cladode, flowering and phonological characteristics of over 100 Opuntia accessions and fruit quality of 45 different accessions were evaluated. Information on fruit characteristics were presented (poster) on the 2005 ASHS meeting. Evaluation of fruit quality will continue in 2006 (last evaluation year). The study will provide information on the NPGS germplasm for nurserymen, small farm growers and the Hispanic, Hmong and Italian heritage home gardeners. Evaluation of half-sib garlic family. Selected economic traits were evaluated in 2 locations (Parlier and Pullman) and compared to the maternal plant (second year completed). The 2005 evaluation was the last one. Data will be summarized during 2006, and a manuscript will be prepared for publication. Literature of this type of study for Allium sativum was not found. The results will provide information and germplasm to garlic breeders and researchers and may result in a germplasm release. Entomology Research Program: Dr. Steve Clement Pea Aphid Outbreak Cycles. Steve Clement has been tracking pea aphid population dynamics since 1986, resulting in the identification of two outbreaks on the Palouse, one in 1990 and one in 1996. Walter Kaiser, retired ARS Research Plant Pathologist, recorded an outbreak in 1983. The pea aphid appeared in 'outbreak numbers' in early summer 2005, bringing the total number of outbreaks over a 23 year period to 4. This outbreak and the vectored viruses resulted in poor seed regeneration in at least one pea nursery established by Clare Coyne. Steve is exploring the possible relationship of outbreak cycles with various abiotic and biotic factors. Pea Weevil Resistance in Pisum sativum x P. fulvum Crosses. The research phase of this project, which started in the early 1990s, is now complete with the evaluations of F 4 material in summer 2005. Briefly, pod-based resistance was detected in hybrids from crosses involving these two Pisum species. In 2006, the large amount of data that has been collected over several years will be summarized so Steve can analyze the results and write a research paper. Cereal Leaf Beetle - Fungal Endophyte - Grass Interactions. The cereal leaf beetle is a fairly new pest in the Pacific Northwest. Its host range includes wheat and many grass species, including taxa maintained by the Pullman PI Station. Low numbers of the beetle have been detected at Central Ferry, so the insect has the potential to harm grass nursery plants. A series of experiments in 2005 quantified feeding damage on replicated plants of tall fescue and perennial ryegrass accessions, with the discovery that damage was extensive and statistically similar on both endophyte-infected (E+) and endophyte-free (E-) plant material of each plant taxa. In addition, larval development was optimal on E- and E- Kentucky 31 tall fescue. This is a cooperative project with Terry Miller, Department of Entomology, Washington State University. Additional experiments will be conducted in 2006 to generate more data on larval development rates on E+ and E- plant material from more temperate grass accessions from the W6 collection. Wild Cicer Germplasm for Pod Borer (Lepidoptera) Resistance. Efforts in 2005 were devoted to developing greenhouse screening methods, securing eggs of beet armyworm from a commercial supplier, and obtaining germplasm from Fred Muehlbauer. This research will be expanded in 2006 to quantify susceptibility of chickpea and wild Cicer hybrid material to neonate and first-instar larvae of the beet armyworm. Retention of Viable Neotyphodium Endophyte in Tall Fescue Accessions Undergoing Seed Regeneration. This research is tied to the W6 germplasm regeneration program. It was initiated in 2002, and the first phase of this project is approximately 75% complete. Details have been given in previous annual reports and will not be repeated here. Briefly, germination rates and endophyte retention rates have been extremely high, which bodes well for the seed regeneration and cleaning procedures in place at the Western Regional Plant Introduction Station. The figures below show some of the initial results of seed germination rates (n=750 seeds/accession) and endophyte retention rates (n=1460 plants) from two tall fescue accessions. Agronomy Research Program: Dr. Richard Johnson Native plant germplasm. We are partnering with the BLM and the US Forest Service to acquire and evaluate key native plant species that are critical for restoration and revegetation efforts. The BLM and Forest service are increasingly interested in establishing properly adapted, native plant materials after disturbances such as logging and fire. Our approach is to collect populations over large areas, understand phenotypic and molecular patterns of variation across the landscape, and maintain and provide the genetic resources needed. Characterization of Mountain Brome (Bromus Marginatus Nees ex Steud.) Germplasm (Cooperative with Vicky Erickson, USFS). Mountain Brome is a key native grass for reclamation and revegetation on federal lands. In the fall of 2003, 1350 were established at two locations for a total of 2,700. In 2004 and 2005, these were characterized for 12 descriptors and entered into spread sheets in preparation for analysis. Also, DNA was extracted from each entry for future molecular analysis. Both univariate and multivariate statistics will be used to gauge collection diversity and test hypotheses as to the extent that genetic variation can be explained by ecological and geographic information. A preliminary analysis showed differences among sites for most traits measured, and wide variation for most. Variation among collection sites was also extensive. This work is cooperative with the US Forest Service to help guide decisions regarding plant adaptation zones and management of Mountain brome for revegetation activities. Enhancement of Kentucky Bluegrass (Poa pratensis L.) Germplasm for Seed Production in No-burn Residue Management Systems. Grass field burning is highly regulated and lower yields from no-burn systems are threatening the grass seed industry in the Inland Northwest. Genetic material is being developed with the goal of providing high yielding types with good turf quality for no-burn residue management systems. Winter Safflower (Carthamus tinctorius L.). Safflower has multiple uses including as an edible oil and for bio-diesel, and is an excellent rotation crop with cereals in semiarid regions. Winter safflower (fall planted) would provide winter cover, allow better control of grass weeds, increase water-use efficiency and seed yield compared to spring sown safflower. We have identified germplasm with enough winter hardiness to be potentially useful for fall planting in many regions and are studying the mechanisms of cold acclimation. Variation within winter hardy safflower is being exploited though selection of improved material; cooperative work with safflower breeders is ongoing. In 2005 data analysis showed that selection has improved winter hardiness. A second year of the same study was established in the fall of 2005 and growth characteristics measured in the fall. In addition, accessions with varying winter hardiness and growth habit were planted to study the physiological basis of the fall cold acclimation process. Measurements of membrane stability, water relations, growth habit and dry weight are being made. Root and shoot tissue is being frozen for future carbohydrate analysis. Non- and sparse flowering orchardgrass (Dactylis glomerata L.) (Cooperative with Mike Casler, ARS, Madison WI, and others) Orchardgrass is a valuable pasture grass in many areas of temperate North America. However, profuse and early flowering in the spring creates management problems and reduces the forage intake of livestock, especially in intensive rotational grazing systems. Two sparse flowering populations WO-SF-B and WO-SF-C have been shown to produce fewer panicles per plant than normally observed in orchardgrass. This material is of interest to understand the physiological nature of the flowering response and also for practical application to improve pasture. In 2004 clones from 98 entries were received for planting at Central Ferry and Pullman. The same material will also be studied in Madison, Corvallis, and Parlier. However, the Pullman and Central Ferry locations, which differ in temperature but not day length, offers a unique contrast in locations. In the fall of 2004, the 98 clones were established at Pullman and Central Ferry locations in randomized completed blocks with four replications resulting in 392 plants. In 2005, for each of the 392 cones at Pullman and Central Ferry, the start of heading and the number of heads per plant was determined. Wide variation in the heading response was noted suggesting important differences among clones and potentially useful breeding material. This will be continued in 2006. Plant Pathology Research Program: Dr. Frank Dugan Relative number of lesions and survival rates were determined for chickpea seedlings adjacent to chickpea debris infested with Didymella rabiei (agent of Ascochyta blight of chickpea) and either treated or not treated with conidial suspensions of Aureobasidium pullulans. The number of lesions on plants adjacent to treated debris was significantly lower than the control, and the number of surviving plants significantly higher than the control. In fulfillment of USDA Cool Season Legume grant (Washington State University, Dept. Plant Pathology, T. Peever PI, pathologist is cooperator). The grant was renewed and analogous experiments were initiated for 2005-2006. A survey of commercially distributed seed garlic from several states and China revealed all shipments to contain one or more fungi documented as pathogenic to garlic (Embellisia allii, Penicillium hirsutum, Aspergillus ochraceus, Fusarium proliferatum, Botrytis sp.). Identification of other fungi in progress. In a field trial with pre-planting dips for garlic cloves (cloves planted 2003, bulbs lifted 2004, stored & rated 2004-2005; data analyzed 2005) fludioxonil and thiophanate methyl gave significant disease reduction relative to controls. MOLECULAR GENETICS LABORATORY, Dr. Ted Kisha Microsatellite analysis of the pea core collection. Collaboration with Clarice Coyne. All DNA samples (~330) previously extracted from the peas core were reprecipitated in 3M NaCl to remove carbohydrates and other impurities. Thirteen primer loci were run using the Licor GeneReadIR PAGE system to complete analysis of 20 microsatellite loci. Analysis of Bromus inermis populations using amplified fragment length polymorphism markers to identity duplicate accessions. Collaboration with Vicki Bradley. Two suspected duplicates of two different accessions were analyzed using AFLP markers. Twenty-four plants from each of the four populations were analyzed. Fragment separation was done using the Beckman CEQ capillary electrophoresis apparatus. All populations were highly polymorphic within. Purported duplicates showed no unique alleles within populations, and the genetic diversity between populations was not statistically significant. Molecular marker analysis of wild Allium acuminatum; Collaboration with Barbara Hellier and R.C. Johnson. Twelve individual plants of Allium columbianum were screened with AFLP primer pairs having 3 or 4 selective nucleotides and combinations thereof. Fragments from primers sets with 3 selective nucleotides on one primer and 4 on the other produced images with a reasonable number of polymorphic bands. The number of bands were still very high, making scoring difficult. Sequence related amplified polymorphic markers (SRAP) were tested to determine if they might provide data easier to reconcile. These gels revealed approximately 10-15 discreet bands per gel and were relatively easy to score compared with AFLP markers. Introgression between cultivated alfalfa and wild relatives in Kazakhstan. Collaborative project with Stephanie Greene. Six wild Medicago sativa ssp varia accessions and five traditional M. sativa varieties collected in Kazakhstan in 2000 were compared using microsatellite markers. The objective is to study the introgression of DNA between cultivated alfalfa and its wild relatives. Six marker loci revealed 119 alleles. AFLP marker analysis of Safflower accessions. Collaborative Project with Vicki Bradley and R.C. Johnson. Twelve individual safflower plants form each of eight accessions were analyzed using AFLP markers. Additionally, bulked samples of 12 plants each from 96 accessions were also analyzed. AFLP marker analysis grouped twelve populations, but showed variantion within accessions as well. Genetic variance within the Arizona Wild Composite was greater than within any other accession, which was expected. Clustering among the bulked accessions was mostly within geographical area of origin. AFLP marker analysis of Reed Canary Grass (Phalaris arundinacea L.) accessions. Collaborative project with Vickie Bradley and Linda Hardesty (WSU). One hundred sixty-seven accessions of P. arundinacea were analyzed using AFLP markers. Preliminary results showed two distinct clusters. Several herbarium specimens were analyzed with good results. This project will attempt to identify whether native P. arundinacea can be identified in the Pacific Northwest. COMPUTER AND IT (G. Pentecost) In 2005, we focused on three areas: improving computer resources within the unit, increasing standardization across different agencies, and efficient use of hardware and software assets. The USDA-ARS and WSU continued to reconfigure their networks along similar models. Both agencies increased the use of collaborative intranet applications. To enable our smooth transition within each system, our unit coordinated communications between ARS and WSU IT personnel. As a result, we began the process of redefining the ARS network identity at WSU in a manner that will satisfy ARS requirements for enterprise-wide digital resource management. As in past years, we upgraded various software packages such as SAS, Adobe Acrobat, Adobe PhotoShop, Microsoft Office Suite, and many others. The current upgrades will meet specific standards for improving communications with the ARS Intranet, as well as within the WSU local area network. Software has only been partially updated on the oldest computers that are mostly used for general data entry. The older computers will gradually be upgraded during upcoming hardware replacements. We finished transferring content from our old web site to the new ARS web site. We next added additional information about the Prosser and Parlier stations to our WRPIS web site. We constructed a separate site for the Phaseolus Genetic Stock Collection. A web site for the 2005 PGOC Meeting provided a centralized location for the meeting's schedule, registration, contact information, and goals. Another web site, for the 2005 NPGS Curators Workshop, served the same purpose and also continues to offer the resultant workshop proceedings. PULLMAN FARM OPERATIONS, Wayne Olson, Farm Manager In January of 2005 we finished the construction of, and moved our Farm Seed Technician into the new office and break room as part of the new Seed Lab located on the Plant Introduction Farm. In 2004 we expanded our Seed Threshing and Seed Cleaning equipment into the new 720 square foot Seed Processing facility from the original 360 square foot seed cleaning facility and started operations. In total we increased our facility from the original 360 square foot facility to 1,368 square feet of Seed Lab and office space. In March 2005 the original threshing room was retrofitted for primarily threshing and cleaning large seeded accessions and crops. The new addition of office, connection corridor, and break room provides an efficient (and weather proof) route for moving dried plants and seed from our drying facility into the new seed lab, as well as an office / communications area for the Unit Seed Technician; and a small, comfortable break room for the Seed Lab employees. In May of 2005, an addition was constructed on the south side of the Seed Drying Building for storage of "Farm and Field Related" materials for the Unit's Curators and Researchers. This new 11,600 cubic foot expansion for storage has helped considerably by moving stored materials out of the drying facility. The storage of large amounts of stored materials in the drying room was impeding the airflow through the building and adding drying time of the plant materials, as well was created a safety issue for foot traffic within the facility. Central Ferry Farm, Kurt Tetrick, Farm Manager In October of 2004 a 25'X 50' pole building were erected a drying shed. This would allow our harvested crops to finish drying down in a cool environment away from the heat and sun of this location. In January Scott McGee, Shawn Vail and I installed R19 insulation on the walls and R33 on the ceiling. We then erected shelving using 2X4 framing and 4X4 uprights for shelving support. The finial shelving assembly was three shelves at a height of 3', 6' and 8' and a depth of 4' going around the entire building. Total shelving space is 984 sq ft. With the larger flat sizes in our transplanting program we were running out of room in our current shade house for hardening of plants. Scott McGee, Shawn Vail and I constructed another shade house. We then remodeled the wooden tables to fit the Styrofoam flats we currently use. We also raised the tables per a request of a curator.

Adair, R., R.C. Johnson, B. Hellier, and W. Kasier. Collecting Taper Tip Onion (Allium acuminatum Hook.) in the Great Basin Using Traditional and GIS Methods. Native Plants Journal. Accepted with minor revisions. Baranger A., B.-Z. Higgins, M-L Pilet-Nayel, C.J. Coyne, G.M. Timmerman, T. Warkentin, A.M. Taylor, and T. Roman. 2006. Dry Pea Breeding for Resistance Against Major Biotic Stresses: From Classical to MAS Breeding. Euphytica. (submitted). Bradley, V.L. 2005. Germplasm Collections: Diversity in the U.S. Cool-Season Grass Collection. Tpi Turf News. March/April 2005:53-57. Coyne C.J., A.F. Brown, G.M. Timmerman-Vaughan, K.E. McPhee, and M.A. Grusak. Refined USDA-ARS Pea Core Collection Based on 26 Quantitative Traits. Pisum Genetics. 37:3-6. Coyne C.J., M. Baum, P.C. Sharma P.M. Gaur, F.J. Muehlbauer, K.E. McPhee, G.M. Timmerman-Vaughan, M-L Pilet-Nayel, A.F. Brown, and R.J. McGee. 2005. Application of Molecular Markers in Food Legumes Breeding. Proceedings of the International Food Legume Research Conference IV. New Delhi, India. Coyne C.J., T. Vincent-Sharp, M.J. Cashman, W. Chen, F.J. Muehlbauer, and N. Mallikarjuna. 2005. A Method for Germination of Perennial Cicer Species. International Chickpea and Pigeonpea Newsletter. 12:28-29. Coyne C.J., L. Razai, B.-K. Baik, and M.A. Grusak. 2005. Variation for Pea Seed Protein Concentration in USDA Pisum Core Collection. Pisum Genetics. 37:7-11. Dugan, F.M. 2006. The Identification of Fungi: An Illustrated Introduction with Keys, Glossary and Guide to Literature. APS Press, St. Paul, MN. Dugan, F.M., and D.A. Glawe. In Press. Phyllactinia guttata is a Host for Cladosporium uredinicola in Washington State. Pacific Northwest Fungi on line. < www.pnwfungi.org >. Dugan, F.M., S.L. Lupien, M. Hernandez-Bello, T.L. Peever, and W. Chen. 2005. Fungi Resident in Chickpea Debris and their Suppression of Growth and Rproduction of Didymella rabiei under Lboratory Cnditions. Journal of Phytopathology.153:431-439. Glawe, D.A., F.M. Dugan, Y. Liu,and J.D. Rogers. 2005. First Rcord and Characterization of a Powdery Mildew on a Member of the Juncaginaceae: Leveillula taurica on Triglochin maritima. Mycological Progress. 4:291-298. Goetz, J., and F.M. Dugan. In Press. Alternaria malorum: a Mini-review with New Records for Hosts and Pathogenicity. Pacific Northwest Fungi on line. < www.pnwfungi.org >. Greene, S.L., 2005. Collecting Legumes In The Largest Remote Region Remaining In The Lower 48 States. Aridus. 17:1-5. Greene, S.L., 2005. U.S. Germplasm Collection of Lotus: Activities Over the Last Decade. Lotus Newsletter. 35:109-111. Greene, S.L., T. Minoura, J.J. Steiner, and G. Pentecost. 2006. WebGRMS: Prototype Software for Web-based Mapping of Biological Collections. Biodiversity and Conservation 15(xx):xxx-xxx. (in review). Hartney, S., D.A. Glawe, F. Dugan, and J. Ammirati. 2005. First Report of Powdery Mildew on Corylus avellana Caused by Phyllactinia guttata in Washington State. Online. Plant Health Progress. doi:10.1094/PHP-2005-1121-01-BR. Hellier, B., H.R. Pappu, and F. Dugan. 2005. Symptoms of Iris Yellow Spot Virus on Wild Onion. APSnet Image of the Week. < apsnet.org/online/archive/2004/iw000049.asp >. Infantino, A., M. Kharrat, L. Riccioni, C.J. Coyne, K.E. McPhee, and N.J. Grünwald. 2006. Screening Techniques and Sources of Resistance to Root Diseases in Legumes. Euphytica. (in press). Johnson, R.C., L. Dajue, and V.L. Bradley. Winter Survival and Autumn Growth in Diverse Safflower Germplasm. Canadian J. Plant Science. In press. Loridon K., K.E. McPhee, J. Morin, P. Dubreuil, M.L. Pilet-Nayel, G. Aubert, C. Rameau, A. Baranger, C.J. Coyne, I. Lejeune-Hénault, and J. Burstin. 2005. Microsatellite Marker Polymorphism and Mapping in Pea (Pisum sativum L.). Theoretical and Applied Genetics. 111:1022-1031. Mimura, M., C.J. Coyne, and T.A. Lumpkin. 2006. Genetic Diversity of Edamame Soybean [Glycine max (L.) Merr.] from Japan, China and the U.S. Genetic Resources and Crop Evolution. (published online January 2006). Muehlbauer, F.J., S. Cho, A. Sarker, CJ. Coyne, P.N. Rajesh, and R. Ford. 2006. Application of Biotechnology in Breeding Lentil for Resistance to Biotic and Abiotic Stress. Euphytica. (in press). Okubara, P.A., K.E. Keller, M.T. McClendon, K.E. McPhee, D.A. Inglis, and C.J. Coyne. 2005. Y15_999Fw, a Dominant SCAR Marker Linked to the Fusarium Wilt Race 1 (Fw) Resistance Gene in Pea. Pisum Genetics. 37:32-35. Pappu, H.R., B.C. Hellier, and F.M. Dugan. 2005. First Report of Onion Yellow Dwarf Virus, Leek Yellow Stripe Virus and Garlic Common Latent Virus in Garlic in Washington State. Plant Disease. 89:205. Pappu, H.R., B.C. Hellier, and F.M. Dugan. In Press. Iris Yellow Spot Virus Infection of Wild Onion Germplasm Accessions in Washington State. Plant Disease. VOL: PP. Pilet-Nayel M.L., F.J. Muehlbauer, J.M. Kraft, R.J. McGee, A. Baranger and C.J. Coyne. 2005. Consistent QTLs in Pea for Partial Resistance to Aphanomyces euteiches Isolates from the United States and France. Phytopathology. 95:1287-1293. Poole, G.J., W.J. Johnston, and R.C. Johnson. 2005. International Turfgrass Society Research Journal 10:565-571. Weiners, R.R., S.-Z. Fei, and R.C. Johnson. 2005. Characterization of a USDA Kentucky Bluegrass (Poa pratensis L.) Core Collection for Reproductive Mode and DNA Content by Flow Cytometry. Genetic Resources and Crop Evolution (Web publication). Books, Chapters, Proceedings, Popular

Afonin, A.N., S.L. Greene, A.N. Frolov, N.I. Dzyubenko, M.M. Levitin, I.Ya. Grichanov, N.N. Luneva, and M.I. Saulich. 2005. A GIS-based Interactive Agricultural Atlas of the Former Soviet Union with Special Reference to Ranges of Agricultural Pest Organisms. In: Crop Protection Conference - Management Aspects of Crop Protection and Sustainable Agriculture: Research, Development and Information Systems, p. 5-7. St.Petersburg - Pushkin, May 31-June 3, 2005. Bradley, V.L., and T.J. Kisha. 2005. Analysis of Bromus Inermis Populations Using Amplified Fragment Length Polymorphism Markers to Identity Duplicate Accessions. In: Molecular Breeding for the Improvement of Forage Crops and Turf: Proceedings of the 4th International Symposium on the Molecular Breeding of Forage and Turf, a Satelllite Workshop of the Xxth International Grasslands Congress. July 2005. Aberystwyth, Wales. P. 267. Brown, A.F., C.J. Coyne, and G.M. Timmerman-Vaughan. 2005. Single-Nucleotide Polymorphisms in Pea (Pisum sativum) and Applications to Germplasm Utilization. Proceedings of the International Food Legume Research Conference IV. New Delhi, India. Clement, S.L., T.L. Griswold, R.W. Rust, B.C. Hellier, and D. Stout. Bee Associates of Flowering Astragalus and Onobrychis Genebank Accessions at a Snake River Site in Eastern Washington. Journal of the Kansas Entomological Society. In press. Clement, S.L., J. Ridsdill-Smith, and S. Cotter. 2005. First-instar Helicoverpa punctigera Larvae: Feeding Responses and Survival on Desi Chickpea and the Wild Relative Cicer bijugum. International Chickpea and Pigeonpea Newsletter. 12:35-37. Coyne, C.J., A.F. Brown, and G.M. Timmerman-Vaughan. 2005. Preliminary Assessment of Allelic Diversity in USDA Pea Core Collection. North American Pulse Improvement Association Biennial Meeting. October 28 & 29. Newark, Delaware. Pisum Genetics. 37:(in press). Dugan, F.M., B.C. Hellier, and S.L. Lupien. 2005. Efficacy of Fungicides against Infections of Garlic in Washington State. Phytopathology Supplement; APSnet. (12-Apr-05). Greene, S.L., A.N. Afonin, A.N. Frolov, N.I. Dzyubenko, M.M. Levitin, I.Ya. Grichanov, N.N. Luneva, and M.I. Saulich. 2005. In Virtual Collection of Agricultural Crops, Their Wild-Growing Relatives and Pest Organisms within the Former Soviet Union. The 5th International Symposium-Electronic Biological Collections: Theory, Standards, and Perspectives of Usage. Zoological Institute & Botanical Institute RAS. September, 11-18, 2005. Hellier, B.C., and M. Pavelka. 2005. Summary of Fertility Characteristics of the USDA Garlic Collection When Grown in Pullman, Wa. [abstract]. Hortscience. 40:1039 Johnson, R.C. Confining Safflower Pollen during Regeneration of Germplasm Seed Stocks. Proceedings: Workshop on the Confinement of Genetically Engineered Crops during Field Testing, September 13-15, 2004, USDA-APHIS Headquarters. In press. (Accepted Aug 2005). Nelson, C., S. Westmoreland, and F. Dugan. 2005. The Variable Sarcodon laevigatus - A Mystery of Shapes and a Wealth of Colors. The Mushroom Dye-Gest. Fall 2005. Nieves-Rivera, Á.M., N.J. Rodríguez, F.M. Dugan, B.R. Zaidi, and E.H. Williams, Jr. In Press. Characterization of Cladosporium oxysporum and C. sphaerospermum using Polyaromatic Hydrocarbons (PAHs) as Their Sole Carbon Source in Tropical Coastal Seawater, in: Recent Research Developments in Multidisciplinary Applied Microbiology 2005: Understanding and Exploiting Microbes and Their Interactions - Biological, Physical, Chemical and Engineering Aspects. Wiley-VCH, Weinheim. Pappu, H.R., B.C. Hellier, and F. M. Dugan. 2005. Iris Yellow Spot Virus Infection of Wild Onion Germplasm Accessions in Washington State. Plant Disease. (Accepted). Abstracts, Reports, Posters

Brenner, D.M., T. Ayala Silva, B.C. Hellier, K.E. Hummer, M.M. Jenderek, L. Fredrick Marek, J.B. Morris, R.L. Nelson, K.R. Reitsma, L.D. Robertson, S.M. Stieve, E.W. Stover, and M.P. Widrlechner. 2005. Germplasm Collection Management and Evaluation for Trait Utilization Genetic Resources of Omega-3 Fatty Acid Crops. American Society of Agronomy Meetings. Brown A.F., C.A. Watt, L. Razai, and C.J. Coyne. 2005. Molecular Diversity of USDA Pea Core Collection using Microsatellites. Model Legume Congress. Asilomar, CA. Brown, A.F., C.A. Watt, L. Razai, and C.J. Coyne. 2005. Refined Pea (Pisum sativum) Germplasm Core Collection: Genetic Diversity as Revealed by RAPD, SSR, and Morphological Trait Data. In ASA-CSSA-SSSA Annual Meetings Abstracts [CD-ROM], p. 8563. Clement, S.L. 2005. Forecasting Pea Aphid Outbreaks on Peas in the U.S. Pacific Northwest: Slam Dunk or Slim Chance? 7th International Symposium on Aphids, Perth, Western Australia. (Abstract). Clement, S.L., H. Sharma, and J. Ridsdill-Smith. 2005. Confronting the Lepidoptera Pod-Borer Problem on Chickpea through International Collaboration and Research. Annual meeting of the Entomological Society of America, Ft. Lauderdale, Florida. (Poster). Coyne C.J., A.F. Brown, and G.M. Timmerman-Vaughan. 2005. Preliminary Assessment of Allelic Diversity in USDA Pea Core Collection. Abstract for North American Pulse Improvement Association Biennial Meeting. October 28 & 29, 2005. Coyne, C.J., C.A. Watt, and L. Razai. 2005. Comparison Of Genetic Diversity Analyses Of The U.S. Pea (Pisum sativum) Core Collection Based On Quantitative Trait Data And Molecular Markers. International Plant and Animal Genome XIII Conference, p. 465. Ellis, D.D., D.M. Skogerboe, C.G. Andre, B.C. Hellier, and G.M. Volk, 2005. Cryopreservation of 12 Allium Sativum (Garlic) Accessions: a Comparison of Plant Vitrification Solutions (Pvs2) and Pvs3. in Vitro Cellular and Developmental Biology - Plants. June 5-7, 2005, Baltimore, Maryland. P. 11-12. Meeting Abstract. Glawe, D.A., F.M. Dugan, R.F. Cerkauskas, L.J. du Toit, S.K. Mohan, and Y. Liu. 2005. Leveillula taurica: An Emerging Plant Pathogen in the Pacific Northwest. Inoculum. 56:21. Glawe, D.A., F.M.Dugan, L.J. du Toit, Y. Liu, and J.D. Rogers. 2005. Leveillula taurica in Washington State: A Case History. Meeting of the Western Soil Fungus Conference and the American Phytopathological Society - Pacific Division, Schedule and Abstracts. June 28 - July 1, 2005. Portland Oregon. Glawe, D.A., J.F. Ammirati, B.E. Callan, F.M. Dugan, L.L. Norvell, and M. Seidl. 2005. The Pacific Northwest Fungi Project: Developing a Collaborative Model for Inventorying Biodiversity on a Regional Basis. Inoculum 56:21. Johnson, R.C. Confining Safflower Pollen during Regeneration of Germplasm Seed Stocks. Workshop on the confinement of genetically engineered crops during field testing. http://www.aphis.usda.gov/brs/confine_workshop/johnson_abstract.pdf. Johnson, R.C., T. Kisha, C. LeClarie-Foiles, and V. Bradley. Characterizing Safflower Germplasm with Aflp Molecular Markers. 2005 Crop Science Socity of America Meeting Abstracts: http://crops.confex.com/crops/2005am/techprogram/P5459.HTM. Watt, C., and C.J. Coyne. 2005. Measurements of Domestication Traits in Perennial Cicer Species. In ASA-CSSA-SSSA Annual Meetings Abstracts [CD-ROM], p. 8477. PUBLICATIONS FROM ADDITIONAL ASSOCIATES Albrigo, L., H. Beck, L. Timmer, and E. Stover. 2005. Development and testing of a recommendation system to schedule copper sprays for citrus disease control. J. ASTM International 2(9): (in press). Aradhya M., J. Dangl, B. Prins, and E. Stover. 2005. Use of genetic markers in grape at the National Clonal Germplasm Repository in Davis, California. First International Grape Genomics Symposium p. 62.(abstract). Aradhya M., J. Dangl, B. Prins, and E. Stover. 2005. Use of genetic markers in grape at the National Clonal Germplasm Repository in Davis, California. Proceedings First International Grape Genomics Symposium (submitted). Aradhya, M.K. Potter, D., and Simon, C.J. 2006. Molecular Phylogeny and biogeography of the walnut genus Juglans (Juglandaceae). Botanical Journal of the Linnean Society (in review). Aradhya, M.K., Potter, D., and Simon, C.J. 2006. Cladistic Biogeography of Juglans (Juglandaceae) based on chloroplast DNA intergenic spacer sequences. In Darwins Harvest  New approaches to the origins, evolution, and conservation of crops. Motley, T.J., Zerega, N., and Cross, H. (eds.). Columbia University Press, New York, 143-170. Aradhya, M.K., Prins, B.H., Dangl, G.S., Simon, C.J., and Stover, E. 2005. Genetic diversity and phylogeographic structure of the genus Vitis: implications for conservation. Abstract submitted to the First International Conference on Crop Wild Relative Conservation and Use, Wednesday 14th - Saturday 17th September 2005, Agrigento, Sicily, Italy. Bai, F. J. Watson, J. Walling, N. Weeden, A.A. Santner and D.A. DeMason. 2005. Molecular characteristic and expression of PsPK2, and PINOID-like gene from pea (Pisum sativum). Plant Sci. 168:1281-1291. Bergman, J.W. N.R. Riveland, C.R. Flynn, G.R. Carlson, D.M. Wichman, and K.D. Kephart. 2005. Registration of Montola 2003 Safflower. Crop Sci 2005 45: 801-802. Bhat, R. G., Colowit, P. M., Tai, T. H., Aradhya, M. K., and Browne, G. T. 2006. Genetic and pathogenic variability in Phytophthora cactorum affecting fruit and nut crops in California. Plant Disease (in press). Boman, B., Zekri, M., Stover, E. 2005. Methods for managing salinity in citrus production. HortTechnology 15:21-26. Brenner, D.M., T. Ayala-Silva, B. Hellier, K. E. Hummer, M. Jenderek, L. F. Marek, J. B. Morris, R. Nelson, K. R. Reitsma, L. D. Robertson, S.M. Stieve, E.W. Stover and M.P. Widrlechner. 2005. Genetic resources of Omega-3 fatty acid crops. Soil and Crop Sci. (abstract). Carter, A., J. Hansen, T. Koehler, X.M. Chen, and R. Zemetra. 2005. Development of a recombinant inbred line (RIL) population in soft white winter wheat. 2005 ASA abstract CD. Ferguson, L., E. Stover, and C. Crisosto. 2005. Problems in fig production and physiology. Third International Symposium on Fig p. 37. (abstract). Ghandi, H., M.I. Vales, C. Watson, C. Mallory-Smith, N. Mori, M. Rehman, R.S. Zemetra, and O. Riera-Lizarazu. 2005. Chloroplast and nuclear microsatellite analysis of Aegilops cylindrica. TAG 111:561-572. Glenna, Leland, Gollnick, Margaret A., and S.S. Jones. Inhumane Opportunity Structures: Teaching Eugenics at United States Land-Grant Universities, 1911-1972. Social Studies of Science. (Submitted November 2005) Hanks, J.D., B.L. Waldron, P.G. Johnson, K.B. Jensen, and K.H. Asay. 2005. Breeding CWG-R crested wheatgrass for reduced-maintenance turf. Crop Sci. 45:524-528. Hansen, J., M. Rehman, C. Mallory-Smith, O. Riera-Lizarazu, and R. Zemetra. 2005 Determining the potential for migration of a herbicide resistance gene via pollen from jointed goatgrass backcrosses. WSCS Abstracts  2005 ASA abstract CD. Hanson, B.D., C.A. Mallory-Smith, B. Shafii, D.C. Thill, and R.S. Zemetra. 2005. Pollen-mediated gene flow from blue aleurone wheat to other wheat cultivars. Crop Sci 45:1610-1617. Hanson, B.D., C.A. Mallory-Smith, W.J. Price, B. Shafii, D.C. Thill, R.S. Zemetra. 2005. Interspecific hybridization: Potential for movement of herbicide resistance from wheat (Triticum aestivum) to jointed goatgrass (Aegilops cylindrica). Weed Tech. 19: 674-682. Hu, Z.-M., X.-L. Wu, S. R. Larson, R. R.-C. Wang, T. A. Jones, N. J. Chatterton, and A. J. Palazzo. 2005. Detection of linkage disequilibrium QTLs controlling low-temperature growth and metabolite accumulations in an admixed breeding population of Leymus wildryes. Euphytica 141: 263-280. Hummer, K.E. and E. Stover. 2005. Trends and fashions in fruit cultivar production. HortTechnology 15: 490-491. Jensen, K.B. 2005. Cytology and fertility of advanced populations of Elymus lanceolatus (Scribn. & Smith) Gould x Elymus caninus (L.) L. hybrids. Crop Sci. 45:1211-1215. Jensen, K.B., and S.R. Larson, and B.L. Waldron. 2005. Registration of Mustang Altai wildrye. Crop Sci. 45: 1168-1169. Jensen, K.B., K.H. Asay, D.A. Johnson, S.R. Larson, B.L. Waldron, and A.J. Palazzo. 2006. Registration of Bozoisky-II Russian wildrye. Crop Sci. 46:986-987. Jensen, K.B., K.W. Maughan, and K.H. Asay. 2006. Genetic introgression between Pseudorogneria spicata (Pursh.) A. Love and Elymus lanceolatus (Scribner and Smith) Gould hybrids. Crop Sci. 46:655-661. Jensen, K.B., M.D. Peel, B.L. Waldron, and K.H. Asay. 2005. Persistence after three cycles of selection in NewHy RS-wheatgrass [Elymus hoffmannii K.B. Jensen & Asay] at increased salinity levels. Crop Sci. 45:1717-1720. Jensen, K.B., S.L. Larson, B.L. Waldron, and D.A. Johnson. 2005. Characterization of hybrids from induced x natural tetraploids of Russian wildrye. Crop Sci. 45:1305-1311. Jensen, K.B., S.L. Larson, B.L. Waldron, and K.H. Asay. 2006. Cytogenetic and molecular characterization of hybrids between 6x, 4x, and 2x ploidy levels in crested wheatgrass. Crop Sci. 46: 105-112. Jones, S.S., S.R. Lyon, K.A. Balow, T.D. Murray, X.M. Chen, B.P. Carter, C.R. Morris, K. Garland Campbell, J.W. Burns, W.F. Schillinger, P.E. Reisenauer, B. J. Goates. 2006. Registration of Masami Wheat. Crop Sci. 46: XX-XX In Press. Jones, SS. 2004. Progress without patents: Agricultural research, no strings attached. Journal of Environmental Law and Litigation. Vol. 19 (2): 463-465. Jones, T. A. 2005. Genetic principles for the use of native seeds. Native Plants J. 5:14-24. Jones, T. A. 2005. Native seeds in commerce. Native Plants J. 6:286-293. Jones, T. A., D. C. Nielson, S. L. Caicco, G. A. Fenchel, and S. A. Young. 2005. Registration of Star Lake Indian ricegrass germplasm. Crop Sci. 45:1666. Lammer, D., Cai, Xiwen, Arterburn, M., Chatelain, J., Murray, T.D., and Jones, S.S. 2004. A single chromosome addition from perennial Thinopyrum elongatum confers a polycarpic, perennial habit to annual wheat. Journal of Experimental Botany. Vol. 55, No 403; 1715-1720. Lee, B., M. Kim, R. R.-C. Wang, and B. L. Waldron. 2005. Relationships among 3 Kochia species based on PCR-generated molecular sequences and molecular cytogenetics. Genome 48: 1104-1115. Li, H.J., M. Arterburn, S.S. Jones and T.D. Murray. 2004. A new source of resistance to Tapesia yallundae associated with a homoeologous group 4 chromosome in Thinopyrum ponticum. Phytopathology. Vol. 94, No. 9; 932-937. Li, H.J., M. Arterburn, S.S. Jones and T.D. Murray. 2005. Resistance to eyespot of wheat, caused by Tapesia yallundae, derived from Thinopyrum intermedium homoeologous group 4 chromosome. Theor Appl Genet. 111: 932-940. Li, Y.L., D.A. Johnson, Y.Z. Su, J.Y. Cui, and T.H. Zhang. 2005. Specific leaf area and leaf dry matter content of plants growing in sand dunes. Botanical Bulletin of Academia Sinica 46: 127-134. Moffet M. and N.F. Weeden. 2005. Pheophorbide a monooxygenase (Pao) is located on LG VII near Amy in pea and lentil. Pisum Genetics 37:24-29. Monaco, T.A., D.A. Johnson, and J.E. Creech. 2005. Morphological and physiological plasticity of Isatis tinctoria: Responses to contrasting light, soil-nitrogen, and water. Weed Research 45: 1-7. Motley, T.J., Cross, H., Zerega, N., and Aradhya, M.K. 2006. Appendix II. Molecular analyses. In Darwins Harvest  New approaches to the origins, evolution, and conservation of crops. Motley, T.J., Zerega, N., and Cross, H. (eds.). Columbia University Press, New York, 370-378. Murphy K., D. Lammer, S. Lyon, B. Carter, S.S. Jones. 2005. Breeding for organic and low-input farming systems: An evolutionary-participatory breeding method for inbred cereal grains. Renewable Agriculture and Food Systems 20: 45-55. Postman, J., K. Hummer, E, Stover, R. Krueger, P.Forsline, L.J. Grauke, F. Zee, T. Ayala-Silva, and B. Irish. 2006. Fruit and nut genebanks in the US National Plant Germplasm System. HortScience (in press). Rainbolt, C.R., D.C. Thill, R.S. Zemetra, and D.L. Shaner. 2005. Imidazolinone-resistant wheat acetolactate synthase (ALS) IN VIVO response to Imazamox. Weed Tech.19: 539-548. Rehman, M. J. Hansen, O. Reira-Lizarazu, C. Mallory-Smith, and R. Zemetra. 2005. Effect of transgene genome location on the risk of gene migration from wheat to jointed goatgrass in wheat x jointed goatgrass backcross progenies. 2005 ASA abstract CD. Rehman, M., J. Hansen, C. Mallory-Smith, O. Riera-Lizarazu, and R. Zemetra. 2005. Potential for gamophytic and/or sporophytic selection for herbicide resistance in wheat x jointed goatgrass backcrosses. WSCS Abstracts  2005 ASA abstract CD. Riera-Lizarazu, O., C.J.W. Watson, R.S. Zemetra, C. Mallory-Smith, and M. Isabel Vales. 2005 Development of a molecular marker linkage map of jointed goatgrass (Aegilops cylindrica). 2005 ASA abstract CD. Souza, E.J., N. Bosque-Pérez, M.J. Guttieri, D.J. Schotzko, S.O. Guy, B. Brown, and R. Zemetra. 2005. Registration of Jerome wheat. Crop Sci. 45: 1161-1162. Stover, E. and M. Aradhya. 2005. Fig genetic resources and research at the U.S. National Clonal Germplasm Repository in Davis, California. Third International Symposium on Fig p. 18. (abstract). Stover, E. and M. Aradhya. 2005. Fig genetic resources and research at the U.S. National Clonal Germplasm Repository in Davis, California. Acta Hort. (submitted). Stover, E. and M. Aradhya. 2005. Fig genetic resources and research at the U.S. National Clonal Germplasm Repository in Davis, California. Acta Hort. (submitted). Stover, E., M. Aradhya, and L. Ferguson. 2005. The fig: overview of an ancient fruit. HortScience 40:953 (abstract). Stover, E., M. Aradhya, B. Prins, J. Dangl, and P. Cousins. 2006. Vitis genetic resources and research at the U.S. National Clonal Germplasm Repository in Davis, California. Ninth International Conference on Grape Genetics and Breeding (abstract). Stover, E., M. Aradhya, C. Crisosto, and L. Ferguson. 2006. Screening the U.S. national fig collection for potential fresh fruit genotypes. HortScience (abstract). Stover, E., M. Aradhya, C. Weeks, and P. Forsline. 2006. Prunus genetic resources and research at the U.S. National Clonal Germplasm Repository in Davis, California. Third International Rosaceae Genomics Symposium (abstract). Stover, E., M. Aradhya, L. Ferguson, and C.H. Crisosto. 2006. The fig: overview of an ancient fruit. J. Amer. Soc. Pomol. (submitted). Stover, E., W, Castle, and C.C.T. Chao. 2005. Trends in U.S. sweet orange, grapefruit, and mandarin-type cultivars. HortTechnology 15:501-506. Stover, E.W., Greene D.W. 2005. Environmental effects on the performance of foliar applied plant growth regulators. HortTechnology 15:214-221. Waldron, B.L., K.B. Jensen, R.D. Harrison, A.J. Palazzo, and T.J. Cary. 2006. Registration of Yakima western yarrow germplasm (Source Identified). Crop Sci. 46:488-489. Waldron, B.L., S.R. Larson, Jensen, K.B., R.D. Harrison, A.J. Palazzo, and T.J Cary. 2006. Registration of Reliable Sandberg bluegrass germplasm (Selected Class). Crop Sci. 46:487-488. Wang, R. R.-C. Registration of TBTE001 and TBTE002. 2006. Thinopyrum amphidiploid genetic stocks differing for leaf glaucousness. Crop Sci. 46: 1013-1014. Wang, R. R.-C., J.-Y. Zhang, B. Lee, K. B. Jensen, M. Kishii, and H. Tsujimoto. 2006. Variations in abundance of 2 repetitive sequences in Leymus and Psathyrostachys species. Genome (published on-line 05/10/06 at http://pubs.nrc-cnrc.gc.ca/) REFEREED PAPERS: Villalobos, W., L. Moreira, K.S. Derrick, M.J.G. Beretta, R.F. Lee, and C. Rivera. 2005. First report of citrus blight in Costa Rica. Plant Disease. 89:108. Lee, R.F., M.G.H. Dekkers, and M. Bar-Joseph. 2006. Development of stable, uniform antigen controls for use in ELISA assays for Citrus tristeza virus. Accepted as a refereed paper in the Proc. 16th Conf. IOCV. Palmieri, M., I. Donis, A.L. Salazar, N. Cruz, A. Paniagua, R.H. Brlansky, A. Guerra-Moreno, K.L. Manjunath, P. Ballance, and R.F. Lee. 2006. Citrus viruses in Guatemala: Application of laboratory-based assays. Accepted as a refereed paper in the Proc. 16th Conf. IOCV. Guerro-Moreno, A.S., K.L. Manjunath, R.H. Brlansky, and R.F. Lee. 2006. Citrus leprosis symptoms can be associated with the presence of two different viruses: cytoplasmic and nuclear, the former having a multipartite RNA genome. Accepted as a refereed paper in the Proc. 16th Conf. IOCV. Ramos, C., J.C. Castillo, O. Fernandez, B. Range., K.L. Manjunath, and R.F. Lee. 2006. Molecular characterization of Citrus tristeza virus isolates from Panama. Accepted as a refereed paper in the Proc. 16th Conf. IOCV. Sieburth, P.J., K.G. Nolan, M.E. Hilf, R.F. Lee, P. Moreno, and S.M. Garnsey. 2006. Discriminatio of stem-pitting Citrus Tristeza Virus isolates from other CTV isolates. Accepted as a refereed paper in the Proc. 16th Conf. IOCV. Roy, A., K.L. Manjunath, and R.H. Brlansky. 2005. Assessment of sequence diversity in the 5' terminal region of Citrus tristeza virus from India. Virus Research. 113:132-142. NON-REFEREED PAPERS: Krueger, R.R., J. Bash, and R.F. Lee. 2005. Dweet mottle virus and Citrus leaf blotch virus. Subtropical Fruit News 3(1):7-9. Krueger, R.R., J. Bash, B. Rangel, and R.F. Lee, 2006. Citrus leaf blotch in California. (IOCV Newsletter). Putter, T., R. Putter, R.F. Lee, and C.N. Roistacher. 2006. EcoPort slide shows on the internet. Accepted as a short non-refereed paper in the Proc. 16th Conf. IOCV. Krueger, R.R., J. Bash, and R.F. Lee. 2006. Phytosanitary status of California citrus. Accepted as a short non-refereed paper in the Proc. 16th Conf. IOCV. ABSTRACTS PUBLISHED: Rangel, B. and R.F. Lee. 2005. Phytoplasmas in Citrus. Phytopathology 95:S86. Hajeri, S., B. Rangel, and R.F. Lee. 2005. Dweet Mottle Disease and Citrus Leaf Blotch Disease may be caused by same virus or different strains of same virus. Phytopathology. 95:S39. Ramos, C., C.N. Roistacher, G.W. Muller, K. Bederski, B. Rangel, and R.F. Lee. 2006. Molecular characterization of Citrus tristeza virus isolates from mild strain cross protection experiments in Peru. Abstract to be published in Proc. 16th Conf. IOCV. Rangel, B., R.R. Krueger, and R.F. Lee. 2006. Current research on Spiroplasma citri in California. Abstract accepted to be published in Proc. 16th Conf. IOCV. Guerra-Moreno, A.S., K.L. Manjunath, R.F. Lee, and R.H. Brlansky. 2005. Citrus leprosis disease is associated with a bipartite positive-sense RNA virus. 2005 Caribbean Division meeting of APS, Costa Rica, June 2005. Manjunath, K.L., E. Rangel, A.S. Guerra-Moreno, R.H. Brlansky, and R.F. Lee. 2005. Serological detection of the cytoplasmic Citrus leprosis virus (CiLV) from infected citrus plants. 2005 Carribbean Division meeting of APS, Costa Rica, June 2005. El-Assar, A.M., R.R. Krueger, P.S. Devanand, and C.T. Chao. 2005. Genetic analysis of Egyptian date (Phoenix dactylifera L.) accessions using AFLP markers. Genetic Resources and Crop Evolution 52:601-607. Brick, M.A., J.B. Ogg, J.J. Johnson, H.F. Schwartz, and F. Judson. 2005. Registration of Grand Mesa Pinto Bean. Crop Sci. 45:413. Volk, G.M., C.M. Richards, A.A. Reilley, A.D. Henk, P.L. Forsline, and H.S. Aldwinckle. 2005. Ex situ conservation of vegetatively-propagated species: Development of a seed-based core collection for Malus sieversii. J Am Soc Hort Sci. 130: 203-210.