Brief Summary of Minutes of Annual Meeting

Call to Order The Regional S-9 Technical Advisory Committee (TAC) was called to order at 1:05 PM on Monday, August 2, 2004, by chairperson Jorge Mosjidis in room 1151 McCarty Hall on the University of Florida campus, Gainesville, FL. Roll Call Each person did a self-introduction including their organizational affiliation and their professional responsibilities. Representatives from some states were not present. Welcome and Opening Remarks Dr. Gerald Arkin welcomed the group via a conference call from his office in Griffin, GA. He could not attend the S9 meeting due to an urgent meeting on Monday evening relating to potential funding of a project on the Griffin campus. Dr. Arkin commended Gary Pederson, the curators and staff, and the S9 Committee for the germplasm repository functioning at its best in many years. He also commented about the relatively stable funding support that the SAES directors have provided over the past few years for conservation and utilization of the germplasm collections. Dr. Arkin reported that the S9 project is the only off-the-top SAES funded project in the southern region. He reviewed the role of the S9 Committee as being (1) providing annual state reports as accountability to the local AES director of germplasm utilization in their state, (2) advise Dr. Pederson and the curators on use and conservation of germplasm, and (3) lobby the local state AES director for continued investment in this program. Dr. Mary Duryea, Assistant Dean for UFL/IFAS, welcomed the group to campus and to Florida. She stated that agriculture was a major source of revenue to Florida, $60 billion annually in agriculture products, second only to tourism. She also stated that IFAS was firmly committed to plant breeding and to germplasm use and conservation. She also stated that UFL had plans to add a $10 million building dedicated to genetic research and education. Ken Quesenberry, 2004 host for the meeting, added his welcome to the group and covered local arrangement details. PGRCU Overview Gary Pederson gave a presentation (Appendix 1)* covering the activities and improvements of the Plant Genetic Resources Conservation Unit (PGRCU) at Griffin during the past year. He reviewed the mission of PGRCU as well as the progress that had been made in backing up the various plant collections since 1996. Dr. Pederson also reported on the domestic and foreign distributions of germplasm from the unit during the past year. Additionally, he reported on the status of funding, staffing, equipment purchases, facilities acquisitions, repairs and maintenance. The committee commented on the continued positive leadership and improvements to the unit under Dr. Pedersons tenure as research leader. Legumes and Miscellaneous Crops: Brad Morris made a presentation (Appendix 2)* on status of regeneration and backup on various specialty uses (e.g., ornamental and human health) of some legume species such as winged bean, sesame, castor bean (ricin), water chestnut, and Crotolaria (protein and fiber). Vegetable Crops and Sweetpotato Bob Jarret described (Appendix 3)* the addition of an enhanced descriptor process of taking digital photos which embody 37/42 descriptors such as whole plant, fruit, and flowers regenerating. He reported that his program was regenerating ~150 accessions/year. He highlighted some of the work being done with okra (digital images), sweet potato (tissue regeneration), and watermelon (regeneration of heirloom varieties for storage in CO). Molecular Lab Rob Dean reported (Appendix 4)* that the most recent molecular marker work had been focused on morphological traits and disease resistance genes in sorghum germplasm. The core collection of sorghum has been characterized for several traits. * Appendices 1, 2, 3, and 4 are located with the 2004 minutes on the S-9 website at http://www.ars-grin.gov/ars/SoAtlantic/Griffin/pgrcu/reports/min2004.pdf Examples of Germplasm Use in University of Florida Programs Eileen Kabelka, Cucurbit Breeding & Genetics, presented background information on important cucurbits in FL such as watermelon, cucumbers, squash and pumpkins. She reported that the market is driven by consumer preferences of size, color, flavor and seedless fruit (watermelon). Traits important in breeding new varieties to meet consumer preferences are disease resistance, fruit quality, uniformity, yield, and nutritional attributes. She cited an example of exotic germplasm utilization in the FL watermelon breeding program of using wild bitter gourd and wild species to obtain disease resistance. Another example she cited was using attributes of squash germplasm accessions and pedigree information to decide which material to use in crosses aimed at new variety development. Ken Quesenberry, Forage Legume Breeding, distributed copies of three publications, New Plants for the South (1961), Value of University of Florida Forage Legume Cultivars to Livestock Production (1999), and New Plants for Florida (2003) that reviewed some of the history of the use of germplasm accessions in crop improvement and variety development for numerous crops that are important in Florida agriculture. Dr. Quesenberry briefly discussed the impact that plant breeding programs have had on FLs agricultural economy. Dr. Barry Tillman, Peanut Breeding, discussed some of the history and goals of FLs peanut improvement program. He reported that the genetic base of the runner type varieties trace back to approximately 13 PIs. He indicated that the market demands flavor and uniformity and as such the market is dominated by a few varieties such as Florunner and GA Green. These have been driving factors for a narrow genetic base. Dr. Tillman reported that much of the utilization of plant introductions in breeding come through sources of disease and pest resistance (e.g., PI 209336 used as a resistant parent for tomato spotted wilt virus in peanut). National Overview Due to flight delays and cancellations, Dr. Peter Bretting was unable to be present for the afternoon meeting but joined the group later in the day. Dr. Ann Marie Thro gave a general overview of the National Plant Germplasm System as follows: I. Background: The National Plant Germplasm System, NPGS, includes: - 4 multistate projects (S-9, W-6, NE-9, NC-7) which are multi-crop collections -6 more single crop collections -the National Center for Genetic Resources Conservation, in Ft. Collins CO -the Genetic Resources Information Network -and many other sites and units. The NPGS is funded primarily by ARS (>90%). ESCOP* participates in the NPGS through regional funds to the 4 multi-state projects and off-the-top funds to one of the single-crop collectors (potatoes, NRSP-6). **ESCOP participates in planning and management of the NPGS via SAES directors who serve as Administrative Advisors: one director per region for the multi-state projects and four directors who serve as AA=s for NRSP-6. Collaboration between ESCOP and ARS provides increased management options and flexibility to the projects, and leverages additional funding and in-kind support. *ESCOP: Experiment Station Committee on Policy. ESCOP is composed of directors of State Agricultural Experiment Stations (SAES). **ESCOP funding varies from 8% to 22% depending on the project II. The NPGS is more valuable today than at any previous time. Molecular tools improve management efficiency and give new power to gene discovery using the collections Consumers are increasingly interested in variety and quality in foods. NPGS collections will be the source of new consumer traits to take advantage of this new market. More restrictions and costs apply to international collecting of plant germplasm; international inventions will limit access in future. Germplasm is recognized by the Federal Government as important for national food security III. Current challenges Federal funding is expected to be flat or declining for some time. ESCOP interest in making funding available to new initiatives Potential for piece-by-piece dis-investment of ESCOP=s participation in NPGS before there is a long-term plan for ESCOP=s engagement with NPGS in future (may limit opportunities) IV. ESCOP and the NPGS - future and long-term Should ESCOP continue to be engaged with planning and management in the NPGS? If so, what mechanisms are available to ESCOP for continued involvement? The present system (4 multistate projects and 1 NRSP): Has strong regional support for some projects Has proven to be an effective way for SAES involvement to add value to the germplasm collections and get them into plant breeding programs and other research, producing benefits for science, educators, farmers and consumers May be the optimum mechanism for the future - or - there may be other options that fit ESCOP's long-term plans Tuesday, August 3, 2004 The group traveled to Citra, FL and toured the Plant Sciences Research and Education Unit. The tour highlighted the peanut, sorghum, cotton, corn, perennial peanut, pigeon pea, horticultural and ornamental research being conducted on the station as well as some of the research facilities and equipment. S9 RTAC Meeting Resumed Chairman Jorge Mosjidis reconvened the group at 11:10 a.m., August 3, 2004 in room 1151 McCarty Hall. Dr. Peter Bretting presented the National Program Staff Report with the activities at the national level (Appendix 2). He reviewed: (1) the general budget situation for NPGS sites, (2) NP reviews, (3) public stakeholder meeting results, and (4) international germplasm issues. He announced that all of the RTACs would be invited to meet with the National Program group in Ames, IA in 2006. Committee Members Discussions Discussion focused on suggestions that could help the unit and the whole system make an even more efficient use of the resources available. (1) Dr. Tom Zimmerman recommended that the CGC reports be sent to S9 TAC members at least one week prior to the annual meeting. This would allow time for committee members to review the accomplishments and status of each project and be in a better position to make helpful suggestions. Dr. Pederson stated that attempts would be made to meet this request in the future. (2) Dr. Tom Stalker asked Dr. Pederson about upcoming constraints for the overall program. Dr. Pederson addressed four areas: (a) Land availability on the Griffin campus is on a year-to-year basis. The solution may be to lease 17 acres across from existing land on the Westbrook farm. On that property there is a need for well for irrigation, pump house and fencing. Dr. Pederson stated that there is land available in Bryon and at some other stations in GA, but the problem is distance. (b)Equipment - some need for additional pollination cages, (c) Additional herbarium specimens of species, especially for grasses. (d) Lists of major priority needs for each curator- needs vary by curators. Some use equipment that reduces labor loads; however, many labor needs are met by shifting farm crew members from one project to another at peak labor demands. Dr. Stalker requested that next year Dr. Pederson provide the committee with a list of priorities for equipment and other needs of the program. Dr. Mosjidis commented that this would be very useful for the S9 TAC members to provide support for the Unit and helping the Unit stay on course. Dr. Stalker reiterated that the purpose of the TAC is give advice. Dr. Mari Marutani asked if the digital photos could be entered into the GRIN network. Dr. Pederson reported that the GRIN data information was entered by a separate group in Beltsville, MD. Tobacco Germplasm Collection Dr. Tom Stalker brought up the issue regarding the tobacco germplasm collection that is being maintained at NC State University. For the past several years, NCSU has maintained the collection of ~2000 accessions + 200 species. The current dilemma is that the person who has served as the curator is departing and there is no person or program to take over the collection. The U.S. Congress has taken the stand that no federal money can go to support tobacco research, even though most of the basic research focuses on tobacco as a model plant or focuses on medical - pharmaceutical research using tobacco as a bio-factory. After some discussion a motion was made by Hussey and seconded by Allen that the S9 TAC recommend that the USDA take back the tobacco collection from NC State. The motion passed. The USDA-ARS representatives abstained from the vote. It was recommended that the Director of NC State Experiment Station take the action item to the next SAES directors meeting for their thoughts and action (especially the tobacco states). Business Session K. Quesenberry moved to approve the minutes as distributed and Zimmerman seconded. The motion passed. Mosjidis moved and Zimmerman seconded that Emerson Shipe be elected as secretary and Fred Allen as chair for the 2005 S9 TAC meeting. The motion passed. The location for the 2005 meeting was discussed and F. Allen volunteered to host the meeting in 2005. Quesenberry moved and Shipe seconded that the meeting will be held in TN in 2005, preferably during the first week of August. The group thanked Dr. Quesenberry for hosting the meeting, and the participating FL faculty for their presentations. Yearly reports from the S-9 TAC were distributed. State representatives were encouraged to complete one if they hadnt done so. Each report should include yearly activities, impact and publication. Meeting was adjourned 12:15 pm (Hussey/Stalker)

The complete accomplishments section for the 2004 S-009 annual report is located at the S-009 website (www.ars-grin.gov/ars/SoAtlantic/Griffin/pgrcu/s9.html). A brief summary of the accomplishments is listed below. USDA, ARS, Plant Genetic Resources Conservation Unit: Plant genetic resources collected or obtained from throughout the world are valuable sources of genetic diversity for use in agronomic and horticultural crop improvement programs in the U.S. This project forms part of a comprehensive nationwide program, National Plant Germplasm System, to preserve plant genetic resources for use today and for use by future generations. Two cowpea lines were identified with resistance to cucumber mosaic virus (CMV) by Graves Gillaspie. These resistant lines could lead to development of cowpea cultivars with improved CMV resistance. Digital images of cowpea flowers and seeds were prepared for inclusion on GRIN. Twenty-five wild peanut accessions collected in Paraguay in 2003 were identified, vegetatively increased, and distributed to the Arachis Research and Evaluation Group by Roy Pittman. Researchers in FL, GA, TX, and Puerto Rico are evaluating the accessions for possible forage and horticulture use. A total of 775 cultivated peanut accessions were selected for regeneration. The entire seashore paspalum grass collection was fingerprinted by Melanie Newman and Ming Li Wang using transferred simple sequence repeat (SSR) markers for more efficient curation of the collection and to aid in marker assisted breeding of this grass. Digital images were taken of grass and bamboo accessions. Genetic variability among genotypes of a lectin from hyacinth bean and sesamin and tocopherol from sesame was determined by Brad Morris in collaboration with AgResearch Consultants (hyacinth bean) and Oklahoma Medical Research Foundation (sesame). Fall regeneration of short-day African clovers was successfully conducted in the greenhouse. Regeneration of 2,100 sorghum accessions in St. Croix and Puerto Rico were conducted by the sorghum curator, John Erpelding. The Sorghum CGC plans to conduct a photoperiod sensitivity evaluation next year of 9,000 sorghum accessions maintained only at Ft. Collins and all accessions insensitive to photoperiod will be moved to Griffin. Accessions insensitive to photoperiod are in most demand by U.S. grain sorghum researchers. Bob Jarret characterized chile pepper species germplasm collections via digital images and descriptors. More than 1,500 vegetable crop accessions were grown for seed regeneration or maintained in tissue culture (sweetpotato). Almost 600 of the 700 sweetpotato accessions in tissue culture have been backed up in Ft. Collins, CO. SSR markers from common legume and grass databases were transferred for use to other legume and grass species including peanut, cowpea, clover, lablab, guar, seashore paspalum, bamboo, and bermudagrass by Ming Li Wang. An HPLC was purchased to characterize variation for phytochemicals among genetic resources within the collection. The -18 C cold room was upgraded with moveable storage shelves to maximize seed storage space in 2003 and maximize seed longevity. Germination tests were conducted on almost 8,000 pepper, sorghum, and 16 other crop accessions. Over 3,000 accessions were sent to Ft. Collins for back up samples. A 60 x 100 metal machine shed was purchased for storage of farm, regeneration, and irrigation equipment. 100 pepper regeneration cages were purchased by ARS. Alabama: Current work focuses on evaluation of the sunn hemp, clovers and Vicia species. Sunn hemp germplasm is being used for development of cultivars for the continental U.S. that can be used as fodder and as cover crops. Florida: Dan Gorbet and Barry Tillman, Marianna, evaluated peanut PIs for resistance to tomato spotted wilt virus (TSWV) and other diseases (536059, 512249, 540866, 497358). Other PIs were evaluated as parents in our peanut CRSP project. Ann Blount and colleagues released two sexual tetraploid lines of bahiagrass, Q4188 and Q4205 (PI 619631 and 619632). Ken Quesenberry and Ann Blount evaluated Wilmington type bahiagrass (PIs 315732, 315733, 315734, and 434189) for forage and turf potential. Ken Quesenberry is evaluating native warm and cool season legumes for forage potential. Gordon Prine collected seed from tall ecotypes of castor bean to preserve adapted Florida genotypes for future research. Dr. Prine plans to release ornamental perennial peanut (Arachis glabrata) PI 262839 (Abrlick) and PI 262840 (Ecoturf) this year and three populations of pigeonpea (Cajanus cajan). Hawaii: Quality and performance trials of HAES macadamia selections are ongoing at the Univ. Hawaii Expt. Station in Kona. Evaluation of the newest HAES selections (862, 879, 887, 896, 900, and 932) is ongoing at the Univ. Hawaii stations in Hilo and Kainaliu. Nutrient content of fruits for two longan cultivars (Biew Khiew and Sri Chompoo) growing at 3 different locations on the island of Hawaii are reported in the complete S-9 report. Louisiana: Blair Buckley, LSU screened the Vigna core collection for reaction to the bacterial blight pathogen Xanthomonas axonopodis pv. vignicola. Wink Alison, LSU, tested alfalfa varieties at the Southeast Station. John Veremis, USDA/ARS, Houma acquired exotic sorghum relatives to make crosses with Saccharum species to improve sugarcane. He planted the core collection of sorghum (2,000 accessions) and evaluated to provide yield data to potential growers. Stephen Boue, USDA, New Orleans will be screening samples soon for unique estrogenic compounds in legumes and other plants. Don La Bonte and Chris Clark evaluated sweetpotato accessions for genetic variability in uptake of Fe and Zn to enhance micronutrient levels to lessen nutritional deficiencies in developing countries. North Carolina: Plant breeding and genetics faculty at NC State University are conducting research on strawberry, blueberry, brambles, tree crops, ornamentals, maize, soybean, peanut, cotton, tobacco, small grains, turfgrass, knead, sweet potato, cucurbits, and other crops. Interdisciplinary teams are incorporating disease resistance, quality factors, or abiotic stress resistance from introduced plant accessions into their improved breeding materials and cultivar development. Germplasm collections include cultivated and/or wild species accessions of Nicotiana and Arachis species, South American maize, soybean, blueberries and sweet potato. Specific projects are reported in the complete S-9 annual report. The curator of the Nicotiana collection recently left the Department of Crop Science and maintenance of this collection may be problematic in the future unless additional resources can be obtained. The collection consists of approximately 2000 cultivated, 200 wild species, and a monosomic series for N. tobaccum. To adequately maintain the collection, a full-time technician and operating support is needed. Oklahoma: C. M. Taliaferro reported bermudagrass accessions have contributed to the breeding of new seed- and vegetatively-propagated cultivars. Yukon and Riviera are new seed-propagated and Patriot is a new vegetatively-propagated turf bermudagrass. Switchgrass accessions (110) were assessed for descriptors and many incorporated into breeding populations for studies of genetic variation and geographic adaptation. Shashi B Sharma, De-Yu Xie and Richard A. Dixon, Samuel Roberts Noble Foundation, Ardmore are working on condensed tannin biosynthesis in legumes including Desmodium uncinatum to introduce condensed tannin biosynthesis in alfalfa leaves. David Porter and Yinghua Huang, USDA-ARS, Stillwater screened 29,541 sorghum accessions from Griffin, GA for their responses to greenbug feeding. The germplasm is a rich gene pool for breeding insect resistance in sorghum. Puerto Rico: Resistance to sorghum anthracnose was identified for 196 accessions from Mali at TARS, Puerto Rico and College Station, TX in collaboration with Louis Prom. Stable anthracnose resistance over multiple locations will provide a more durable source of resistance. TARS-PT03-1, a small seeded pinto dry bean was released. In collaboration with Graves Gillaspie, Griffin, GA, 12 cowpea lines are being screened for yield and tolerance to cowpea chlorotic mottle, CMV, and blackeye cowpea mosaic virus. The regeneration of 2100 sorghum accessions with low seed viability was conducted at the Germplasm Introduction Research Unit, St. Croix. A total of 220 sorghum, 50 cowpea, 12 cucurbits, 15 winged bean, and 15 Leucaena accessions were planted at Isabela, Puerto Rico, for seed increase. Also, 420 sorghum and 42 corn accessions were planted at St. Croix for seed increase and release from quarantine. Photographs of 1500 sorghum panicles and 200 images of cacao pods, banana racemes, and mamey sapote fruits were entered into GRIN. South Carolina: A total of 29 watermelon accessions were distributed to Judy Thies and 364 cowpea accessions to Paul Berland, USDA Vegetable Laboratory, Charleston. Soybean PIs 548316 (Cloud), 548658 (Lee 74), 548402 (Peking), 88788, 89772, 90763, 209332, and 437654 were used in a greenhouse bioassay to categorize two South Carolina populations of soybean cyst nematode (Heterodera glycines). A glyphosate-tolerant soybean line, SC00-1075, was released in 2003 by the South Carolina Agricultural Experiment Station and is marketed as AGS 825 RR. Tennessee: The following projects are being conducted at the University of Tennessee in which novel or exotic germplasm lines are being utilized. Fred Allen and Richard Johnson crossed a soybean fibrous root line, PI 416937, and cultivars with differing leaflet orientation to develop recombinant inbred isolines with different leaflet orientation and root trait combinations and compare their water-use relative to seed yield. Vincent Pantalone is developing new soybean populations with enhanced genetic diversity. The TAES registered the soybean variety 5002T (PI 634193), developed from the cross Holladay x Manokin. Four new cross hybridizations are being initiated to expand diversity for applied variety development. Early generation lines from the Germplasm Enhancement of Maize (GEM) project, coordinated through the USDA Maize project at Iowa State University, are crossed with elite adapted corn lines. Dennis West, Univ Tenn and Major Goodman, NCSU have 891 experimental hybrids from the GEM project in yield trials in Tennessee. They also have 377 nursery rows of GEM material for inbreeding and selection. Janice Zale and Ludmila Ohnoutkova, Univ. Tenn., and Camille Steber, USDA/ARS, Pullman , WA have transformed one wheat germplasm line and one millet accession using the floral dip. Neal Stewart is evaluating the biosafety of genetically modified plants using the crop canola (Brassica napus) as a model to assess transgene flow, persistence and consequence to free-living wild relatives. He is evaluating the molecular mechanisms conferring tolerance in glyphosate-tolerant horseweed (Conyza canadensis). Texas: Peggy Thaxton and Wayne Smith, College Station, derived converted race stocks (CRS) of cotton from photoperiodic, primitive Gossypium hirsutum accessions collected in Mexico and Central America. With David Stelly, advanced interspecific backcross populations BC1F3 and BC1F2 between G. hirsutum with G. tomentosum or G. mustelinum were developed and are being evaluated. 116 CRS are being screened for resistance to fleahopper, aphid, and whitefly, seed and seedling disease complex, and nematodes. Studies were initiated with the CRS or their BC progeny for salt tolerance, drought tolerance, seed-seedling disease resistance, and fleahopper resistance. Selections were made in 2003 from a large population of F4 PR of G. hirsutum and sea island (G. barbadense). This material produced several very high fiber quality lines. John Gannaway, Lubbock, is increasing seed to initiate screening of wild cotton germplasm from collections at College Station (8500), Russia (6500), and CIRAD in France (2500) for insect resistance (aphids, lygus, thrips), disease (black root rot) as well as drought and salinity tolerance. Yin-Tung Wang reports that Phalaenopsis orchids for summer and fall flowering must be grown in air-conditioned greenhouses. Phalaenopsis Will Bates, Phalaenopsis Alameda, and Doritaenopsis Cherry Bates orchid hybrids were registered with the Int. Orchid Reg. Authority, Kew, England. Mark Burow reported wild peanut species were used to generate interspecific breeding lines that developed COAN and NemaTAM. These materials are currently being used to develop leaf-spot resistant peanut cultivars. In addition, Bolivian landraces are being examined for early maturity and an African accession is being used for developing early maturity Spanish and runner populations to combine earliness with high yield and a high oleic/linoleic ratio.

USDA  Plant Genetic Resources Conservation Unit Wang, M. L., A. G. Gillaspie, M. L. Newman, R. E. Dean, R. N. Pittman, J. B. Morris and G. A. Pederson (2004) Transfer of simple sequence repeat (SSR) markers across the legume family for germplasm characterization and evaluation. Plant Genetic Resources 2 (2): 107-119. Morris, B. 2003. Bio-functional legumes with nutraceutical, pharmaceutical, and industrial uses. Economic Botany 57:254-259. Morris, J.B. 2004. Legumes: Nutraceutical and pharmaceutical uses. p. 651-655. In R.M. Goodman (ed.) Encyclopedia of Plant and Crop Science. Marcel Dekker, New York. Fery, R. L., J. A. Thies and A. G. Gillaspie. 'KnuckleHull-VNR', a crowder-type southernpea resistant to blackeye cowpea mosaic virus and root-knot nematodes. HortScience 39(1):183-184. 2004. Levi A., C. E. Thomas, M. Newman, O.U.K. Reddy, X. Zhang, and Y. Xu. 2004. ISSR and AFLP markers differ among watermelon cultivars with limited genetic diversity. Journal of the American Society for Horticultural Science. V. 129. p. 553-558. Pederson, G.A. 2003. Data submission by users of plant genetic resources. Agronomy Abstracts (CD). Wang, M. L., N. Barkley, R. Dean, C. Holbrook, and R. N. Pittman. 2004. Transfer of Medicago EST-SSRs to peanut for germplasm evaluation and cross-species cloning. The 36th Annual Meeting of American Peanut Research and Education Society, San Antonio, TX, P6. Wang, M. L., R. Dean, J. Erpelding, and G. Pederson. 2004. Molecular genetic evaluation of sorghum germplasm differing in response to three fungal diseases: Anthracnose, grain mold and rust. Plant Biology 2004, Orlando, FL, p128. Gillaspie, A. G., Jr., M. L. Wang, D. L. Pinnow, and R. N. Pittman. Development of a grid RT-PCR method for detection of Peanut mottle (PeMV) and Peanut stripe viruses (PStV) in peanut germ plasm. Phytopathology 94:S34. 2004. (Abstract) Morris, J.B., K.M. Moore, and J.B. Eitzen. 2003. Velvetbean: A special-purpose legume for important agricultural and medicinal applications. Agron. Abstrs. (CD-ROM). Morris, J.B., K.M. Moore, and J.B. Eitzen. 2003. Guar: A potential new crop in Georgia, USA. The Assoc. for the Advancement of Ind. Crops Abstrs. p. 48. Levi A., C. E. Thomas, M. Newman, and X. Zhang. 2004. Preferential segregation of linkage-groups produced quasi-linkage in F2 mapping population of watermelon. Plant and Animal Genome XII Proceedings. p. 755. Levi A., C. E. Thomas, M. Newman, X. Zhang, Y. Xu, and T. Wehner. 2003. Massive preferential segregation and nonrandom assortment of linkage-groups produce quasi-linkage in an F2 mapping population of watermelon. HortScience. p. 782 (181). Wang, M. L., G. Gillaspie, M. Newman, R. Dean, R. Pittman, B. Morris and G. Pederson. 2004. Shuffling DNA markers within the legume family for germplasm characterization and evaluation. Plant and Animal Genome XII, San Diego, p. 203. Tolford, S., J.G. McArthur, and J.B. Morris. 2004. FRIL lectin variability among Lablab purpureus genetic resources. Joint Meeting of the 39th North American Alfalfa Improvement Conference and the 18th Trifolium Conference Abstrs. (www.naaic.org/index.html.). Alabama Mosjidis, Jorge A., Stephanie L. Greene, Kimberly A. Klingler and Alexandr Afonin. 2004. Isozyme diversity in wild red clover populations from the Caucasus. Crop Sci. 44 (2):665-670. Wehtje, Glenn, and Jorge A. Mosjidis. 2004. Response of sericea lespedeza to Imidazolinone. Proc. 57th Annual Meeting of the South. Weed Sci. Soc. Jan 26-28, 2004, Memphis, TN. Kalburtji K.L., J.A Mosjidis. and A.P Mamolos. 2003. Effects of temperature on seedling emergence of several sericea lespedeza genotypes. p. 35. 7th International Clonal Plant Workshop. August 1-5, 2003, Kuusamo, Finland. Mosjidis, J.A 2003. Genetic diversity as affected by breeding origin in red clover cultivars. Annual Meeting Abstracts. ASA, CSSA, SSSA. CD-ROM. Florida Blount, A.R., R.N. Gates, P.L. Pfahler, and K.H. Quesenberry. 2003. Early plant selection effects on crown traits in Pensacola bahiagrass with selection cycle. Crop Sci. 43:1996-1998. Quarin, C.L., M.H. Urbani, A.R. Blount, E.J. Martinez, C.M. Hack, G.W. Burton, and K.H. Quesenberry. 2003. Registration of Q4188 and Q4205, sexual tetraploid lines of bahiagrass. Crop Sci. 43:745-746. Quesenberry, K.H., J. M. Mullaney, A.R. Blount, R.S. Kalmbacher, and J.G. Norcini. 2003. Characterization of physiological and morphological variability in buffalo clover. Soil Crop Sci. Soc. Fla Proc. 62:66-69. Blount, A.R., K.H. Quesenberry, R.O. Myer and R.N. Gates. Bahiagrass Breeding. 2003. Sod based rotation conference. 20-21 February 2003, Quincy, FL Quesenberry, K.H. and R.L. Smith. 2003. Production of sexual tetraploid bahiagrass using in vitro chromosome doubling agents. P. 145. Molecular Breeding of Forage and Turf, Third International Symposium. 18-22 May 2003, Dallas TX. Breman, J.W.*, A.R. Blount, K.H. Quesenberry, T.R. Sinclair, R.N. Gates, R.D. Barnett, and S.W. Coleman. 2003. Discrimination of cold damage in bahiagrass. Soil Crop Sci. Soc. Florida. Proc. 62:94. Breman*,J.W., A.R. Blount, T.R. Sinclair, and K.H. Quesenberry. 2003. Cold tolerance variability in bahiagrass. Agron Abstr. CD-ROM, C06-blount622907-O. Carvalho*, M.A., and K.H. Quesenberry.2003 Arachis pintoi seed production in Florida. Prodeeding 35th APRES. 8-11 July 2003 Clearwater Beac, FL Carvalho*, M.A., K.H. Quesenberry, D.W. Gorbet.2003 Seed production and fatty acid profile in Arachis pintoi. Agron Abstr. CD-ROM, C08-carvalho237434-P. Mislevy, P., K.H. Quesenberry, M.J. Williams, A.R. Blount. 2003. Influence of harvest management on yield and persistence of rhizoma peanut on wet tropical soils. Agron Abstr. CD-ROM, C03-mislevy243766-P. Quesenberry, K.H., R.L. Smith, J.M. Mullaney, and A.R. Blount. 2003. Development, identification, and characterization of tetraploid bahiagrass. Agron Abstr. CD-ROM, C01-quesenberry529179-P. 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Archives of Biochemistry and Biophysics 422:91102. Xie, De-Yu, Shashi B. Sharma, Nancy L. Paiva, Daneel Ferreira, and Richard A. Dixon. 2003. Role of Anthocyanidin Reductase, Encoded by BANYULS in Plant Flavonoid Biosynthesis. Science 299 :396-399. Tanner, Gregory J., Kathy T. Francki, Sharon Abrahams, John M. Watson, Philip J. Larkin, and Anthony R. Ashton. 2003. Proanthocyanidin biosynthesis in plants: Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA. J. Biol. Chem. Bartel, Bonnie, and Seiichi P. T. Matsuda. 2003. Seeing Red. Science 299: 352-353. Puerto Rico Goenaga, R. and Rivera, E. (2004). Yield and fruit quality of carambola cultivars grown at three locations in Puerto Rico. Abstract ASHS-1846-hna-03. Goenaga, R., Rivera-Amador, R., and Almodovar, C. (2004). Yield of papaya irrigated with fractions of Class A pan evaporation in a semiarid environment. J. Agric. Univ. PR. 88(1-2). In press. South Carolina Shipe, E.R., J.D. Mueller, S.A. Lewis, P.F. Williams, Jr., and R.K. Stephens. 2003. Registration of Santee soybean. Crop Science 43(6): 2305-2306. Robbins, R.T., E.R. Shipe, L. Rakes, L.E. Jackson, E.E. Gbur, and D.G. Dombek. 2002. Host suitability of soybean cultivars and breeding lines to reniform nematode tests conducted in 2001. Supp. to J. of Nematology 34(4): 378-383. Bohac, J. R., D. M. Jackson, J. D. Mueller, M. Sullivan, and P. D. Dukes. 2003. Notice of release of Liberty, a multiple pest resistant, dry fleshed sweetpotato cultivar. U. S. Department of Agriculture and South Carolina Agricultural Experiment Station. USDA, ARS, Washington, DC, September 15, 2003. Bohac, J. R., D. M. Jackson, J. D. Mueller, P. D. Dukes, and J. M. Schalk. 2000. Notice of release of Patriot, an insect resistant, copper rose skinned, orange fleshed sweetpotato cultivar. U. S. Department of Agriculture and South Carolina Agricultural Experiment Station. USDA, ARS, Washington, DC, Dec. 19, 2000. Bohac, J. R., D. M. Jackson, P. D. Dukes, and J. D. Mueller. 2002. Ruddy: a multiple-pest resistant sweetpotato. HortScience 37:993 994. Bohac, J. R., P. D. Dukes, Sr., J. D. Mueller, H. F. Harrison, J. K. Peterson, J. M. Schalk, D. M. Jackson, and J. Lawrence. 2001. White Regal, a multiple pest and disease resistant, cream fleshed, sweetpotato. HortScience 36:1152 1154. Kays, S. J., W. J. McLaurin, Y. Wang, J. R. Bohac, P. D. Dukes, and D. M. Jackson. 2000. Notice of release of GA90-16 sweetpotato breeding line. Georgia Agricultural Experiment Station and U. S. Department of Agriculture. USDA, ARS, Washington, DC, June28, 2000. Kays, S. J., W. J. McLaurin, Y. Wang, P. D. Dukes, J. R. Bohac, and D. M. Jackson. 2001. GA90 16: A nonsweet, staple type sweetpotato breeding line. HortScience. 36:175-177. U. S. Department of Agriculture and South Carolina Agricultural Experiment Station. 1999. Notice of release of Ruddy multiple pest resistant, red skinned, orange fleshed sweetpotato. USDA, ARS, Washington, DC, June 28, 1999. (Cultivar developed by J. R. Bohac, P. D. Dukes, J. D. Mueller, and D. M. Jackson). Jackson, D. M., J. R. Bohac, J. Lawrence, and J. D. Mueller. 1999. Multiple insect resistance in dry-fleshed sweet potato breeding lines for the USA and Caribbean. Pages 274-280 In Progress in IPM CRSP Research, Proceedings of the Third IPM CRSP Symposium, 15-18 May 1998, Blacksburg, VA. Virginia Polytechnic Institute and State University, Blacksburg, VA, 327 pp. Lawrence, J., D. M. Jackson, and J. R. Bohac. 1999. Evaluation of USDA sweet potato breeding lines as a potential tactic for managing sweet potato pests in the Caribbean and USA. Pages 298-304 In Progress in IPM CRSP Research, Proceedings of the Third IPM CRSP Symposium, 15-18 May 1998, Blacksburg, VA. Virginia Polytechnic Institute and State University, Blacksburg, VA, 327 pp. Jackson, D. M., J. R. Bohac, and J. D. Mueller. 2003. 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Thaxton, P. M. and C. W. Smith. 2004. Registration of Tamcot 22, Crop Sci. Accepted. Thaxton, P. M. and C. W. Smith. 2004. Registration of TAM 96WD-18, Crop Science. Accepted. Thaxton, P. M. and C. W. Smith. 2004. Registration of TAM 96WD-69s, Crop Science. Accepted.