SAES-422 Multistate Research Activity Accomplishments Report

Sections

Basic Information
Additional Info

Status: Approved

Basic Information

Project No. and Title: WERA_OLD20 : Virus and virus like diseases of fruit trees, small fruits and grapevines
Period Covered: 06/01/2005 to 05/01/2006
Date of Report: 06/29/2006
Annual Meeting Dates: 05/08/2006 to 05/10/2006

Rodrigo Almeida, "rodrigo@nature.berkeley.edu" - UC Berkeley; Ralph Cavalieri, "cavalieri@wsu.edu" - Washington State University; Brenda Conley, "conleyb@inspection.gc.ca" Canadian Food Inspection Agency; Ken Eastwell, "keastw@tricity.wsu.edu" - Washington State University; Blake Ferguson, "fergusonb@inspection.gc.ca" - Canadian Food Inspection Agency; Violet Galvin, "galvinv@inspection.gc.ca" - Canadian Food Inspection Agency; Deborah Golino, "dagolino@ucdavis.edu" - UC Davis; Charlene Green, "greenc@inspection.gc.ca" - Canadian Food Inspection Agency; Lauri Guerra, "lguerra@prosser.wsu.edu" - Washington State University; John Halbrendt, "jmh23@psu.edu" - Pennsylvania State University; Bill Howell, "bhowell@tricity.wsu.edu" - Washington State University; John Hu, "johnhu@hawaii.edu" - University of Hawaii; Delano James, Gayle Jesperson, "Gayle.Jesperson@gov.bc.ca" - BCMAFF; Ray Johnson, "JohnsonRC@inspection.gc.ca" - Canadian Food Inspection Agency; Gary Kinard, "pgqogk@ars-grin.gov" - USDA, ARS; Bruce Kirkpatrick, "BCKIRKPATRICK@ucdavis.edu" - UC Davis; Chuck Lemmon, "clemmon@inspection.gc.ca" - Canadian Food Inspection Agency; Roberto Michelutti, "micheluttir@agr.gc.ca" - AAFC; Thierry Poiré, "poiret@inspection.gc.ca" - Canadian Food Inspection Agency; Joe Postman, "jpostman@ars-grin.gov" - USDA, ARS; Hélène Sanfaçon, "SanfaconH@agr.gc.ca" - AAFC; Annemiek Schilder,"schilder@msu.edu" - Michigan State University; Dan Thompson, thompsonda@inspection.gc.ca -Canadian Food Inspection Agency; Mike Tiffany, "miket@agdia.com" - Agdia;

Brief Summary of Minutes of Annual Meeting

For photos of the participants at this meeting please contact eyeates@wsu.edu. They are in .tif format.

Legacy attachment file:

[Minutes]

Accomplishments

1. Viruses involved in the pineapple mealybug wilt disease and their biology have been determined. This was critical for the development and incorporation of cultural strategies, screening technologies at the tissue culture or propagation level, genetic engineering for virus resistance, and pest control including ant and mealybug control in pineapple production. 2. Transgenic plums (Prunus domestica) have been engineered to provide silencing of the Plum pox virus genome, and hence resistance to this virus. 3. It was postulated that infection of a transgenic plant by a virus unrelated to the virus to which the plant is resistant may result in a loss of resistance; this could present a major limitation in the utilization of resistance based on this technology. It was demonstrated that Tomato ringspot virus could not revert already established gene silencing, one of the underlying principles of genetically engineered resistance to viruses. 4. Hemagglutinin was identified as an important gene involved in the pathogenicity of Xylella fastidiosa.

Impacts

The Hawaiian pineapple industry has converted to newer hybrids which have lower incidences of mealybug wilt. Strategies that target control of virus and the mealybug vectors are being utilized to minimize the increase in virus incidence and work towards eradication. This is essential for sustaining the local Hawaii pineapple industry as well as the growing subsidiaries in foreign lands.
Resistant cultivars provide options for continued fruit production in areas that are impacted by Sharka, the disease caused by Plum pox virus. Resistant cultivars also serve as genetic resources for further variety development by incorporating them into breeding programs.
This result(that Tomato ringspot virus could not revert already established gene silencing)suggests that resistance to PPV in transgenic plum is robust and cannot be broken by infection by ToRSV, a virus known to occur in peaches and plums.
This result (that Hemagglutinin was identified as an important gene involved in the pathogenicity of Xylella fastidiosa) provides one of the first opportunities to initiate unconventional control strategies for the devastating diseases of peach and grape caused by these bacteria.

Publications

Li, R., R. Mock. 2005. An improved reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of two cherry flexiviruses in Prunus spp. Journal of Virological Methods. 129:162-169.

Martin, R.R., K.C. Eastwell, A. Wagner, S. Lamprecht, and I.E. Tzanetakis. 2005. Survey for viruses of grapevine in Oregon and Washington. Plant Disease. 89:763-766.

Perez, E.Pp, D.M. Sether, M.J. Melzer, J.L. Busto, C. Nagai, and J.S. Hu. 2005. Characterization and control of pineapple mealybug wilt associated Ampeloviruses. Acta Horticulturae. 702:23-27.

Sether, D.M., M.J. Melzer, J.L. Busto, F. Zee, and J.S. Hu. 2005. Diversity and mealybug transmissibility of Pineapple mealybug wilt associated viruses found in pineapple. Plant Disease. 89:450-456.

Tzanetakis, I.E., J. Postman, and R.R. Martin. 2005. Characterization of a novel member of the family Closteroviridae from Mentha spp. Phytopathology. 95:1043-1048. Tzanetakis, I.E., J. Postman, and R.R. Martin. 2005. A member of the Closteroviridae from mint with similarities to all three genera of the family. Plant Disease. 89:654-658.

Zhang, S.C., G. Zhang, L. Yang, J. Chisholm, and H. Sanfaçon. 2005. Evidence that insertion of Tomato ringspot virus NTB-VPg protein in endoplasmic reticulum membranes is controlled by two domains: a C-terminal transmembrane helix and an N-terminal amphipathic helix. Journal of Virolology. 79:11766-11775.

Book chapters and Reviews:

Sanfacon, H., G. Zhang, J. Chisholm, B. Jafarpour, and J. Jovel. 2006. Molecular biology of Tomato ringspot nepovirus, a pathogen of ornamentals, small fruits and fruit trees. In Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues (1st Edition). Teixeira da Silva, J. (Ed.). Global Science Books, London, UK. 3:540-546.

Sanfaçon, H. 2005. Replication of positive-strand RNA viruses in plants: Contact points between plant and virus components. Canadian Journal of Botany. 83:1529-1549.

Le Gall, O., T. Iwanami, A.V. Karasev, A.T. Jones, K. Lehto, H. Sanfaçon, J. Wellink, T. Wetzel, and N. Yoshikawa. 2005. Family Comoviridae. In Virus Taxonomy: The classification and nomenclature of viruses. The eighth report of the International Committee on Taxonomy of Viruses. 807-818.

Le Gall, O., T. Iwanami, A.V. Karasev, A.T. Jones, K. Lehto, H. Sanfaçon, J. Wellink, T. Wetzel, and N. Yoshikawa. 2005. Family Sequiviridae. In Virus Taxonomy: The classification and nomenclature of viruses. The eighth report of the International Committee on Taxonomy of Viruses. 793-798.

Le Gall, O., T. Iwanami, A.V. Karasev, A.T. Jones, K. Lehto, H. Sanfaçon, J. Wellink, T. Wetzel, and N. Yoshikawa. 2005. Genus Cheravirus. In Virus Taxonomy: The classification and nomenclature of viruses. The eight report of the International Committee on Taxonomy of Viruses. 803-805.

Le Gall, O., T. Iwanami, A.V. Karasev, A.T. Jones, K. Lehto, H. Sanfaçon, J. Wellink, T. Wetzel, and N. Yoshikawa. 2005. Genus Sadwavirus. In Virus Taxonomy: The classification and nomenclature of viruses. The eighth report of the International Committee on Taxonomy of Viruses. 799-802.

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