SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Juan Manuel Alvarez, University of Idaho, Aberdeen, ID; Dallas Batchelor, WA; Greg Bohach, University of Idaho, Moscow, ID; Chuck Brown, USDA-ARS, Prosser, WA; Tom Bewick, USDA-CSREES, D.C.; Jim Crosslin, USDA-ARS, Prosser, WA; Nick David, Oregon State University, Hermiston, OR; Rob Davidson, Colorado State University, Center, CO; Stacy Gieck, Oregon State University, Hermiston, OR; Darren Hall, USDA-ARS, Aberdeen, ID; Phil Hamm, Oregon State University, Hermiston, OR; Dan Hane, Oregon State University, Hermiston, OR; Gary Franc, University of Wyoming, Laramie, WY; Stewart Gray, USDA-ARS, Ithaca, NY; Sherry Laug, Idaho Crop Improvement, Idaho Falls, ID; Jim Lorenzen, University of Idaho, Moscow, ID; Ved Malik, USDA-APHIS; Steve Marquardt, ND; Mel Martin, Simplot, WA; Joe Munyaneza, USDA-ARS, Wapato, WA; Roy Navarre, USDA-ARS, Prosser, WA; Phil Nolte, University of Idaho, Idaho Falls, ID; Cindy Nolte, University of Idaho, Idaho Falls, ID; Hanu Pappu, Washington State University, Pullman, WA; Mark Pavek, Washington State University, Pullman, WA; Lisa Piche, North Dakota State University; David Ragsdale, University of Minnesota; Gary Secor, North Dakota State University; Rue Snell, Colorado State University, Center, CO; Babu Srinivasan, University of Idaho, Aberdeen, ID; Mike Sun; Montana State University, Bozeman, MT; Chet Sutula, Agdia, Elkhart, IN; Colleen Thompson, Idaho Crop Improvement, Idaho Falls, ID; Jonathan Whitworth, USDA-ARS, Aberdeen, ID.

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Accomplishments

Title: Detection of Xylella fastidiosa and phytoplasmas in processing potatoes affected by a new disorder. Issue: A new disorder of processing potatoes grown in the south-central United States, California, and Mexico has been identified. This disorder as been termed zebra chip because of the dark stripes and streaks produced in potato chips prepared from affected crops. To date, 50,000 acres or more have been affected and losses to one grower in Texas alone are estimated at $2 million. Total losses of $20 million in six states have been estimated. What has been done: Discussions among federal and state scientists have led to research efforts to identify the cause of the zebra chip disorder. WERA-089 researchers have associated the bacterium Xylella fastidiosa with many of the affected plants. Another pathogen, a phytoplasma related to the stolbur organism, has been found in some of the affected crops as well. Research efforts to identify the insect or insects responsible for transmission and confirmatory pathogenicity tests of these pathogens are underway. Impact: This is the first time X. fastidiosa has been associated with a disease of potatoes. The stolbur-like phytoplasma also has not previously been associated with potatoes. The discovery of X. fastidiosa, in particular, is of major concern because strains of this pathogen cause serious diseases of many crops including Pierces disease of grapes and variegated chlorosis of citrus. Some strains of X. fastidiosa are considered select agents by USDA-APHIS. Therefore, this pest is of trade and quarantine significance and could potentially cause significant economic losses in many crop plants. The complete understanding of the etiology of zebra chip will allow development of management strategies that currently do not exist. Primary impacted areas: Research and Extension Funding sources: University support through Hatch Act and State funds, USDA-ARS research funds, National Potato Council, Frito-Lay Corporation, numerous growers, and state potato commissions. Contact information: Greg Bohach, Idaho Agricultural Experiment Station, University of Idaho, Moscow, ID 83844. Phone: 208-885-7173. Email: agres@uidaho.edu. Title: Potato virus Y (PVY) diversity and the effects of PVY on crop production and trade. Issue: PVY includes numerous virus strains which reduce yield and crop quality to different degrees. Recently, PVY strains have appeared in the US that cause internal symptoms in potato tubers. These new strains confound accurate detection, identification, and control methods. They therefore have adversely affected seed potato production, eliminating some growers because certification standards could not be met. Understanding the differential impact of these diverse virus strains on different potato cultivars and accessing their potential impact on trade and quarantine issues is critical. What has been done: In 2004, WERA-089 members were instrumental in developing a management plan for PVY that standardized testing methods among various agencies in the US and Canada. More recently, the management plan has served as a basis for further refining of the testing and identification system to include Mexico (NAFTA) and trading partners in the Far East. Additionally, scientists belonging to WERA-089 have developed improved molecular methods for the rapid detection and identification of these diverse virus strains. Impact: Trade barriers between the US and Canada, and more recently Mexico, have been reduced. Efforts to further open markets in the Far East are continuing. Improved methods of detection and identification of PVY strains have been developed. Primary impacted areas: Research and Extension. Funding sources: University support through Hatch Act and State funds, USDA-ARS, USDA-APHIS, National Potato Council, certified seed growers, state potato commissions. Contact information: Greg Bohach, Idaho Agricultural Experiment Station, Iniversity of Idaho, Moscow, ID 83844. Phone: 208-885-7173. Email: agres@uidaho.edu.

Impacts

  1. This is the first time X. fastidiosa has been associated with a disease of potatoes. The stolbur-like phytoplasma also has not previously been associated with potatoes. The discovery of X. fastidiosa, in particular, is of major concern because strains of this pathogen cause serious diseases of many crops including Pierce‘s disease of grapes and variegated chlorosis of citrus. Some strains of X. fastidiosa are considered select agents by USDA-APHIS. Therefore, this pest is of trade and quarantine significance and could potentially cause significant economic losses in many crop plants. The complete understanding of the etiology of zebra chip will allow development of management strategies that currently do not exist. Funds for this work are derived from University support through Hatch Act and State funds, USDA-ARS research funds, National Potato Council, Frito-Lay Corporation, numerous growers, and state potato commissions.
  2. Trade barriers between the US and Canada, and more recently Mexico, have been reduced. Efforts to further open markets in the Far East are continuing. Improved methods of detection and identification of PVY strains have been developed. Funds for this work are derived from University support through Hatch Act and State funds, USDA-ARS, USDA-APHIS, National Potato Council, certified seed growers, state potato commissions.

Publications

Alvarez, J.M. and P.J.S. Hutchinson, 2005. Managing hairy nightshade to reduce potato viruses and insect vectors. Outlooks on Pest Management Journal. 16 (6): 249-252. Alvarez, J.M. and R. Srinivasan. 2005. Evaluation of hairy nightshade as an inoculum source for the aphid-mediated transmission of potato leafroll virus. Journal of Economic Entomology. 98: 1101-1108. Crosslin, J.M., G.J. Vandemark, and J.E. Munyaneza. 2006. Development of A real-time, quantitative PCR for detection of the Columbia Basin potato purple top phytoplasma in plants and beet leafhoppers. Plant Dis. 90:663-667. Lorenzen, J.H., T. Meacham, P.H. Berger, P.J. Shiel, J.M. Crosslin, P.B. Hamm, and H. Kopp. 2006. Whole genome characterization of Potato virus Y isolates collected in the western USA and their comparison to isolates from Europe and Canada. Arch. Virol. 151:1055-1074. Munyaneza, J.E., J.M. Crosslin, and J.E. Upton. 2006. Beet leafhopper (Hemiptera: Cicadellidae) transmits the Columbia Basin potato purple top phytoplasma to potatoes, beets, and weeds. J. Econ. Entomol. 99:268-272. Crosslin, J.M., P.B. Hamm, P.J. Shiel, D.C. Hane, C.R. Brown, and P.H. Berger. 2005. Serological and molecular detection of tobacco veinal necrosis isolates of Potato Virus Y (PVY-N) from potatoes grown in the western United States. Am. J. Potato Res. 82:263-269. Crosslin, J.M., J.E. Munyaneza, A. Jensen, and P.B. Hamm. 2005. Association of beet leafhopper (Hemiptera: Cicadellidae) with a clover proliferation group phytoplasma in Columbia Basin of Washington and Oregon. J. Econ. Entomol. 98:279-283.
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