SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Washington, 4 Utah, 6 Texas, 15 South Carolina, 19 Oklahoma, 21 New York, 27 Nebraska, 32 Montana, 33 Missouri, 37 Mississippi, 41 Louisiana, 46 Iowa, 49 Illinois, 50 Florida, 67 Connecticut, 75 Colorado, 79 California, 89 Arkansas, 93

W5171-Complile-2025-final.docx

Accomplishments

 

 

Impacts

  1. W-5171 Multi-State Research Project Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock IMPACTS Washington - Impacts Objective 1 A growing body of research in rodent models highlights the significant role of paternal factors in fetal development, but comparable studies in livestock species remain limited. As global weather patterns continue to shift, environmental stressors experienced by sires—such as heat or cold exposure—may have profound effects on sperm epigenetic programming and subsequent embryonic and fetal development. Defining these paternal influences, as well as their long-term programming effects on offspring growth, efficiency, and overall performance, is therefore critically important for improving livestock productivity, resilience, and sustainability under changing climatic conditions. Utah – Impacts Objective 1 • Enhanced genome stability in IVF blastocysts with CPEB1 supplementation may improve in vitro blastocyst quality and developmental potential in vivo. • Investigate open regions in EGA related gene expression from biopsy samples for pregnant and non-pregnant embryo transfers, as well as those that result in pregnancy loss or successful term development could help identify key EGA events critical for SCNT embryo development to term. • Exploring the role of serum in embryo development and EGA in the SCNT embryos would help develop an improved culture medium to support SCNT embryo development both in vitro and in vivo. • LOPU is becoming a good alternative source for SCNT sheep production, especially highly valuable genetically engineered animals used for pharmaceutical protein production and animal models of human diseases. • Improving the birth of cloned lambs through natural delivery using a long-induction protocol could enhance long-term survival and increase the overall SCNT efficiency and animal production. • Identifying mitochondrial function and embryo development with melatonin and FLI supplementation provides insight into strategies that may improve oocyte resilience and reproductive outcomes in cattle exposed to elevated environmental temperatures. Texas – Impacts Objective 1. 1. Understanding the role of the paternal histone epigenome in embryogenesis is critical as we further appreciate how the health, diet, and environment of the sire can program the embryo for implantation and long-term health and development. These studies optimize and develop new methodologies to study histone post-translational modifications in bovine sperm, especially extended semen. They further characterize histone PTMs present in bovine testes and sperm, and find conservation amongst other mammalian species. South Carolina – Impacts Objective 1: The dependence on mitochondrial efficiency at different embryonic developmental stages is better understood. In addition, through these projects, the laboratory furthered the education of undergraduate and postdoctoral trainees in the in vitro production of mammalian embryos and in the role of mitochondria in this system. Oklahoma - Impacts Objective 1: • The novel microRNA and tRNA-derived fragment (tRF) data generated during bovine preimplantation development provide valuable insights into the developmental biology processes of early embryogenesis in cattle. The long-term goal of this research is to improve cattle production systems and alleviate the impact of reproductive challenges in cattle operations. • Embryonic stem cells (ESC) are pluripotent cells derived from the inner cell mass (ICM) of blastocysts, with the potential to differentiate into most cell types, including gametes, given appropriate conditions. The development of stable conditions for culturing and differentiation of bovine and bison ESC holds significant potential in veterinary medicine (e.g., regenerative medicine) and for accelerating genetic improvements, particularly when combined with assisted reproductive technologies (ART). Objective 2: • Given the importance of pigs as models for xenotransplantation, our findings establish optimized electroporation parameters using a NEPA21 electroporator in porcine fibroblast cell lines, providing a foundation for future genome-editing studies using this electroporator and cell lines. New York - Impacts Objective 1: This project addresses a pressing and critical issue impacting the U.S. cattle industry. Globally, there has been a significant shift toward utilizing assisted reproductive technologies (ARTs) to enhance animal reproductive efficiency. In 2018 alone, 1,129,041 bovine IVF embryos were transferred, accounting for two-thirds of all embryo transfers that year. While ARTs offer powerful tools to improve fertility, their efficiency remains suboptimal, limiting their widespread commercial adoption. Despite the recognized importance of this issue, there has been limited functional genomic research to understand the epigenetic mechanisms underlying early embryonic development. This project aims to bridge this gap through three key projects: • Project 1: Establish protocols to profile the epigenetic regulator MOF and its associated marker H4K16ac during bovine embryonic development. Project 1 Fills a fundamental knowledge gap by establishing protocols to profile an epigenetic regulator MOF and H4K16ac during bovine embryonic development. • Project 2: Investigate the mechanisms driving developmental rate differences between male and female embryos. Project 2 will identify the underlying mechanisms behind the developmental rate difference between male vs. female embryos. • Project 3: Characterize the functional role of transposable elements in bovine early development. Project 3 will understand the functional role of transposon elements during bovine early development. Collectively, these projects will provide valuable insights into embryonic epigenetics and offer a unique opportunity to develop a targeted biomarker assessment system. This system has the potential to enhance fertility and reproductive efficiency, addressing a critical challenge for the cattle industry. Objective 2: Nebraska - Impacts Montana - Impacts OBJECTIVE 1 Identification and flux quantification of the novel cysteine biogenesis mechanism revealed previously unknown processes by which cells can use to support redox homeostasis. Remarkably, our work over the past year show that this pathway is not exclusive to TR/GR-null cells but, rather, can be activated in most or perhaps all cells under conditions of stress that cause elevation of intracellular cystine levels and/or compromised disulfide reductase capacity. These are likely to be important in embryonic development, which we plan to investigate in future studies. OBJECTIVE 2 Nothing to report for 2025. Missouri - Impacts Under Objective 1 • Manipulating cathepsin D level in early embryos is a promising approach to improve the efficiency of IVP technology in cattle. • Identifying new oocyte reprogramming factors may suggest novel somatic reprogramming pathway. • Expand our understanding of pregnancy recognition in pigs. Under Objective 2 • Use of Cas9 variants can support precise gene editing. Mississippi - Impacts Under objective 1 Several significant impacts on male fertility and breeding practices are as follows: • The study reveals that boar seminal plasma metabolomic profiles differ between summer and winter, impacting semen quality, and suggests that environmental factors are key to reproductive success in swine. • Identifying differentially expressed metabolites such as ergothioneine, dopamine, and lysoPC highlights their roles in sperm functions like energy metabolism, antioxidant defense, and capacitation. These metabolites could act as potential biomarkers for evaluating semen quality. • The differences in metabolomic profiles between high-motile and low-motile sperm offer valuable insights into the metabolic pathways affecting sperm's ability to survive chilled storage, suggesting potential markers (e.g., glyceric acid) for improving sperm preservation methods. • Increased dietary arginine resulted in higher semen volume and total sperm counts, suggesting potential benefits for swine production. Although overall sperm quality measures did not significantly change, the effects on the seminal plasma proteome indicate that further research is needed. • Research on age-related changes in spermatozoa highlights the importance of understanding how aging impacts fertility, with many proteins showing different expression levels across age groups, affecting motility and fertilization. • The study of extracellular vesicles (EVs) in seminal plasma, combined with near-infrared spectroscopy, offers innovative non-invasive methods for evaluating semen quality, which could improve breeding practices in the swine industry. Louisiana - Impacts Objective 1. These results provide insight into follicular environment changes during the oocyte maturation phase which will be helpful in optimizing in vitro maturation systems for cattle. Objective 2. These exploratory experiments potentially provide an additional cryoprotectant for use during cryopreservation of bovine oocytes and embryos, thus enhancing the utility of cryopreservation of female gametes in cattle breeding applications. Iowa - Impacts Illinois - Impacts Objective 1 1. Oocyte Maturation We showed that heat-stress (HS) can compromise the recruitment of ovarian follicles early in a new follicular wave in Holstein dairy cattle. Further, dominant follicle removal before ovum-pickup is beneficial to oocyte number and quality and produced more embryos after IVF. 1. Embryogenesis Our results highlight the potential of precise embryo-estrus alignment to increase the number of live calves produced per IVF run. Our findings suggest that administering GnRH or P4 in recipients with CLs sized from 18 to 22 mm does not improve pregnancy success. The ubiquitous detection of 2,5-dimethyl-4-pyrimidinamine in embryos suggests widespread environmental exposure. Spatial RNA sequencing does uncover novel insights into the in situ biology of the trophoblast, epiblast, and inner cell mass during early bovine embryonic development. Objective 2 1. Transgenic Animals NA Other The findings indicate that cultured ASCs and uBMSCs have the highest osteogenic potential out of the different MSC populations we tested. 3D-printed intestinal stents prevent abdominal sepsis even in the presence of an intestinal defect Florida - Impacts OBJECTIVE ONE Zongliang, Jiang • We have identified a novel mitochondria stress eliminating mechanism regulated by METTL7A that occurs during the acquisition of oxidative stress in embryo in vitro culture. This discovery lays the groundwork for the development of METTL7A as a promising therapeutic target for IVF embryo competence. • Our study provides a valuable resource of spatiotemporal translational regulation in mammalian pre-implantation development and highlights a previously uncharacterized translation initiation factor critical for early embryos. Daigneault, Brad • Data from our program has provided a paradigm-shift in sperm biology by describing modification to the methylome of post-ejaculatory sperm. These data are likely to impact many fields of reproductive biology by suggesting that alterations to the fluids encountered within the male and female reproductive tract may elicit unknown consequences on sperm function with potential carry over implication to embryo development. • The implementation, adaptation and demonstrated efficacy of high-resolution sperm respirometry for modeling bull fertility is likely to provide high impact to the bovine industry as a tool to more accurately estimate bull fertility prior to artificial insemination. These findings have revealed a new sperm trait that is highly related to fertility and further suggests that antiquated methods of motility are inferior to more direct assessment of mitochondrial sperm function. OBJECTIVE 2 Zongliang, Jiang • We report the derivation and long-term culture of bovine extraembryonic endoderm cells (XENs) from pre-implantation embryos. Importantly, this study not only demonstrates the utility of bovine XEN models in elucidating the mechanistic features of early bovine embryogenesis, but also develops an improved bovine blastocyst-like structures (blastoids) technology for the creation of novel assisted reproductive technologies. Daigneault, Brad • Optimization of nanoparticle technology use in bovine embryos for overexpression and gene-editing initiatives has provided a new technology for preimplantation embryo research that offers a low-cost, non-immunogenic, non-viral, and biologically inert method that does not require advanced training for embryo development and gene editing approaches towards advancing livestock production. Connecticut - Impacts 1. In human IVF, pre-implantation genetic testing based on aneuploidy has not been found to increase pregnancy or live birth rates. Our data on ploidy correction in early embryos should shed lights on the long-held observation that the early embryos can correct its ploidy and return to the default ploidy of 2N. 2. While numerous methods have been tested to separate bovine X- and Y-bearing sperm, most of them are either impractical or have failed in repeatability. We tried to confirm a recently reported chemical method but failed to find it repeatable in the mouse, bovine and human. 3. In bovine, generating genetically modified animals have not been achieved with the use of tetraploid complementation. Our study on triploid complementation may be a good alternative approach to generate transgenic animals in one generation. Colorado - Impacts Objective 1 Investigating the impact of genetic and pharmacological approaches to modulate NRF2-mediated oxidative stress response in bovine preimplantation embryos will provide a unique opportunity to enhance survival and mitigate the long-term impact of preimplantation period oxidative damage on fetal development and offspring health. Moreover, the study will identify genes and genome regions which can be targets for future interventions to enhance viability of embryos from assisted reproductive technologies. Moreover, the research on extracellular vesicles-mediated molecular signaling in ovarian follicle and oocytes in Dr. Tesfaye lab will facilitate the development of diagnostic markers associated with maternal physiology and embryo developmental competence and future potential therapeutic application of EVs in assisted reproductive technologies. The use of bovine oviduct organoid technology will allow the generation of physiologically relevant EVs as molecular cargo in embryo oviduct communication for future application in ART to enhance in vitro embryo production yield and quality. Results of the study on pathogens in bovine IVF systems were presented at the International Embryo Technology Society (IETS) in January 2024. These data were used in an assessment on safety of in vitro produced embryos that is currently being conducted by the Health and Safety Advisory Committee (HASAC) of the IETS. Further discussion of the data and next steps will be addressed at the IETS meeting in January 2025. The goal is to provide evidence-based import/export standards that are accessible to the global livestock industry. Objective 2 Thyroid hormone is necessary to support both fetal and placental development during pregnancy. Maternal supply of TH to the fetus early in pregnancy is necessary before the fetus can produce TH itself. The placenta uses thyroid hormone to regulate metabolic processes, which includes using 60-80% of the oxygen and glucose it takes up from the maternal circulation. Regulation of placental function by thyroid hormone during pregnancy is complex with the potential to regulate proliferation, differentiation, hormone production, invasion and angiogenesis. Impaired placental development and function is an underlying cause of fetal growth restriction. The causation and progression of placental insufficiency is not well understood and requires additional studies that investigate the mechanisms of placental function. Our objective is to reduce DIO2 protein in trophoblast cells to determine the impact on placenta function. It is our long-term goal to determine the causes behind impaired placental function, and how placental-insufficiency manifests itself in FGR. We will test if impairment of trophoblast expression of DIO2, DIO3 or SBP2 will result in significant placental and fetal growth restriction by mid-gestation, setting the stage for functional placental insufficiency and FGR. Understanding how thyroid hormones are regulated by the placenta is critical to understanding placental function during FGR. California - Impacts OBJECTIVE 1 • This work advances the field of livestock pluripotent stem cells and opens new avenues to use stem cells for biotechnology applications. OBJECTIVE 2 • The dual guide approach increases the efficiency of gene editing via CRISPR/Cas9. • Transfer of gene edited embryos with acceptable pregnancy rates. • Embryo biopsy used for sexing and assessment of editing status allowed for greater efficiency by only transferring embryos with the desired genotype. Arkansas - Impacts

Grants, Contracts & Other Resources Obtained

Publications

W-5171 Multi-State Research Project

Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock

PUBLICATIONS

Washington – Publications

None

Utah – Publications

  1. Refereed articles/book chapters

(* indicates the corresponding and/or senior author, italic font indicates contribution by an undergraduate student, and italic underlined font indicates contribution by a graduate student or postdoctoral fellow).

  1. Leir, S-H., Tkachenko, S., Paranjapye, A., Meckler, F., Van Wettere, A.J., Kerschner, J.L., Viotti Perisse, I., Marriott C.M., Patrick, T., Liu, Y., White, K.L.,  Polejaeva, I.A., Harris, A.* (2025) Cellular imbalance in proximal and distal lung of CFTR−/− sheep in utero and at birth Molecular Medicine  31:231.
  2. Meckler, F., Van Wettere, A.J., Umrigar A., Bebek G., Leir S-H, Viotti Perisse, I., Lovrenert, K., White, K.L., Polejaeva, I.A., Harris, A.*. (2025) Molecular phenotypes in early gallbladder and liver disease in the CFTR−/− sheep highlight aspects of cystic fibrosis-relevant hepatobiliary disease. Physiological Genomics. 57:682-694.
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

Renata Blocher. PhD. Dissertation title: Approaches to improve in vitro oocyte maturation and somatic cell nuclear transfer embryo developmental potential

Ian Bunderson. MS. Thesis title: Effect of resveratrol and commercial serum-free media on ovine oocyte quality and cloned embryo development

Jada Hansen. MS. Thesis title: Development and optimization of ovine in vitro fertilization for enhanced embryo development

  1. Abstracts
  2. Trowbridge, V., Blocher, R., Patrick, T., Perisse, IV., Marriott, C., Mustafa, Y., Miller, K., Polejaeva, I.A., Liu, Y.* (2025). Effect of cytoplasmic maturation and CPEB1 mRNA injection on DNA methylation patterns in bovine IVF embryos. Reproduction, Fertility and Development. 38:
  3. Liu, Y., Grow, E., Viotti Perisse, I., Patrick, T., Thomas, A., Stott, R., Cairns, B., Polejaeva, I.A., White, K.L.* Impact of Early Biopsy and Chromatin Accessibility on the Development of Cloned Embryos in cattle. Proceeding of Society for the Study of Reproduction Annual meeting.
  4. Miller, K., Hanson, J., Patrick, T., Liu, Y., Thomas, A., Perisse, I.V., Stott, R., Marriott, C., Blocher, R., Trowbridge, V., Polejaeva I.A,, White K.L.* (2025). Effects of serum and commercial serum-free medium on development of in vitro-produced (IVP) embryos in cattle Reproduction, Fertility and Development. 38:
  5. Stott, R., Liu, Y., Marriot, C., Patrick, T., Blocher, R., Regouski, M., Perisse I.V., Polejaeva, I.A.* (2025). Effect of parturition induction methods on delivery of cloned lambs. Cinical Theriogenology. 17:44.
  6. Miscellaneous publications (semi-technical/lay publications)

Texas - Publications

  1. Refereed articles/book chapters

Ing, N.H., Curley Jr., K.O., Welsh Jr., T.O., Johnson, L., Luense, L.J., Clement, T.M. “Anatomy and Physiology of the Male Reproductive System and Potential Targets of Toxicants.” In Comprehensive Toxicology, 3rd Edition, Elsevier Science, 2025

  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

Dodd, A.R. Mapping histone acetylation throughout bovine spermatogenesis and preimplantation embryo development. Master’s Thesis, May 2025

  1. Abstracts

Luense, L.J. The role of the paternal histone epigenome in preimplantation embryo development, International Embryo Technology Society, Fort Worth, TX, January 22, 2025

Dodd, A.R., Luense, L.J., Mapping histone acetylation throughout bovine spermatogenesis and preimplantation embryo development, Texas Forum for Reproductive Sciences, April 2025, Houston, TX

  1. Miscellaneous publications (semi-technical/lay publications)

South Carolina – Publications

  1. Refereed articles/book chapters 
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations
  1. Abstracts

Vogler, I., Summersett, S., Mueller, V., Zarzaca, I., Culler, H., Hersom, M.J., Checura, C.M. Photobiomodulation of Bovine Embryos at 72 Hours Post-fertilization. 38th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Salvador do Bahia, Brazil. Anim Reprod, 22 (3):96, 2025.

Mueller, V., Gainey, L., Gambardella, T., Summersett, S., Vogler, I., Elgarresta, C., Culler, H., Hersom, M., Kordus, R., Checura, C. In Vitro Fertilization In Different Species. Poster presentation at Clemson University 20th Annual Focus on Creative Inquiry Forum, Clemson, SC, USA. 2025. 

Elgarresta, C., Fusco, A., Strawbridge, E., Wang, Y., Checura, C., & Kordus, R. The Effects of Different Sperm Preparation Techniques on Reactive Oxygen Species and DNA Fragmentation. 27th Annual College of Reproductive Biology Symposium, Las Vegas, NV, USA. 2025.

  1. Miscellaneous publications (semi-technical/lay publications)

Oklahoma - Publications

  1. Refereed articles/book chapters
  1. G.N. Moraes, T. Gull, A.C. Ericsson, M.O. Caldeira, T.J. Evans, S.E. Poock, M.C. Lucy. Evaluating differences in uterine microbiome and inflammatory status at 1 month postpartum associated with metritis and antibiotic treatment. Journal of Dairy Science. 2025 Sep 18, https://doi.org/10.3168/jds.2025-26403.
  1. Amorim, S.T., Stafuzza, N.B., Cifuentes, D.C., Moraes, J.G.N., Dos Reis, B.R., Messmann, R., Camaripano, L. and Baldi, F. Genomic Study for Pregnancy Loss in Brahman Cattle. Journal of animal science, 2025, DOI: 1093/jas/skaf326
  1. O. Caldeira, J.G.N. Moraes, T.T. Nguyen, J.C.C. Silva, I. Sellmer Ramos, S.E. Poock, T.E. Spencer, and M.C. Lucy. Impact of early postpartum metritis and systemic antibiotic treatment on the biochemistry and morphology of the bovine uterus at one month postpartum. Biol Reprod. 2025. DOI: 10.1093/biolre/ioaf146 
  1. McDonald K, Drum JN, Moraes JGN, Melton C, Ojeda-Rojas OA, Venturini M, Gonella-Diaza AM, Prather RS, Ortega MS. Effect of FGF2, LIF, IGF1 supplementation on pregnancy success following embryo transfer of in vitro derived embryos. Theriogenology. 2025 Jun 10:117533. DOI: 1016/j.theriogenology.2025.117533
  1. Caldeira MO, McDonald KS, Martinez ES, Moraes JGN, Ramos IS, Poock SE, Ortega MS, Lucy MC. Short-and long-term effects of uterine disease on oocyte developmental capacity in postpartum dairy cows. Theriogenology. 2025 Jul 1;240:117413.
  1. Kiser JN, Seabury CM, Neupane M, Moraes JGN, Herrick AL, Dalton J, Burns GW, Spencer TE, Neibergs HL. Validation of loci and genes associated with fertility in Holstein cows using gene-set enrichment analysis-SNP and genotype-by-sequencing. BMC genomics. 2025 Feb 21;26(1):174.
  1. Sellmer Ramos, M. O. Caldeira, S. E. Poock, J.G.N. Moraes, M. C. Lucy, and A.L. Patterson. Adenomyosis and fibrosis define the morphological memory of the postpartum uterus of dairy cows previously exposed to metritis. JDS Communications. 2025 doi.org/10.3168/jdsc.2024-0633
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

MS Thesis: 2

  1. Lopes, M. J. A. 2025. Derivation and characterization of hybrid bovine × bison embryonic stem cell lines. M.S. Thesis. Oklahoma State Univ., Stillwater, OK.
  1. Bonmann, N. F. F. 2025. CRISPR-Cas9-mediated alpha-Gal knockout in porcine fetal fibroblast cells. M.S. Thesis. Oklahoma State Univ., Stillwater, OK.
  1. Abstracts
  1. Bönmann N.F. F., lamberti, G. S. E., De Moraes, G. F., Lopes, M. J. A., Smith, R. M., Lucas, C.G., Moraes, J.G.N. CRISPR-Cas9-Mediated Alpha-Gal Knockout in Porcine Fetal Fibroblast Cells. 2025 Totusek Lectureship, November 21st, 2025, Oklahoma State University, Stillwater, Oklahoma.
  1. Lopes MJA, Murphy GL, Wilkinson JJ, Bonnman NFF, Botigelli RC, Londono-Vasquez D, Denicol AC, Hagen DE, Moraes JGN. 2025. Derivation and Characterization of Hybrid Bovine x Bison Embryonic Stem Cells. A Closer Look. AETA 2025 Annual Convention.
  1. Murphy G. L., Goldkamp A. K., Lopes M. J. A., Bonmann N. F. F., Lucy M. C., Hagen D. E., Moraes J. G. N. 2025. Let-7 microRNAs: Potential key modulators of bovine preimplantation development. 2025 AFS Research Day.
  1. Murphy G. L., Goldkamp A. K., Lopes M. J. A., Bonmann N. F. F., Lucy M. C., Hagen D. E., Moraes J. G. N. 2025. Alterations in miRNA and tRNA-derived fragment (tRF) expression during bovine conceptus elongation. FAPC Research Symposium. 
  1. Smith R.M., Bönmann N.F. F., de Moraes G. F., Lucas C.G., Moraes J.G.N. CRISPR-Cas9-Mediated Alpha-Gal Knockout in Porcine Fetal Fibroblast Cells. 2025 Freshman Research Scholars Symposium, April 17th, 2025, Oklahoma State University, Stillwater, Oklahoma.
  1. Snow M., Lopes M. J. A., Wilkinson. J. J, Murphy G. L., Botigelli R. C., Londoño-Vásquez D., Denicol A. C, Hagen D. E., Moraes J.G.N. Bovine-bison stem cell validation through embryoid body formation. 2025 Freshman Research Scholars Symposium, April 17th, 2025, Oklahoma State University, Stillwater, Oklahoma.
  1. Wilkinson, J., M.J.A. Lopes, G.L. Murphy, R.C. Botigelli, D. Londono-Vasquez, Z. Jiang, A.C. Denicol, D.E. Hagen, and J.G.N. Moraes. Establishment and validation of novel embryonic stem cell lines derived from Bison blastocysts. 2025, 28th Annual Phi Zeta Research Day. Oklahoma State College of Veterinary Medicine. Stillwater, OK. April 3, 2025.
  1. Wilkinson, J., Lopes, M. J. A., Murphy, G. L., Botigelli, R. C., Denicol, A. C., Hagen, D. E., & Moraes, J. G. N. 2025. Establishment and validation of novel embryonic stem cell lines derived from hybrid bovine and bison blastocysts. AAVMC Veterinary Scholars Symposium, Spokane, WA. Poster Presentation
  1. G.L. Murphy, A.K. Goldkamp, M.J.A. Lopes, N.F.F. Bonmann, M.C. Lucy, D.E. Hagen, J.G.N. Moraes. Alterations in miRNA and tRNA-derived fragment expression during bovine conceptus elongation. 2025. Annual Meeting of the International Embryo Technology Society (IETS), January 18-22, 2025, Fort Worth, Texas, USA.
  1. Miscellaneous publications (semi-technical/lay publications)

New York - Publications

  1. Refereed articles/book chapters
  1. Fang, Y. Su, R. Meisel, J. Walters, T. Gamble, E Randolph, X. Yu, M. Shi, G. Li, J. Zhang, IISAGE Consortium, X.C. Tian, Y. Tang, J.E. Duan, Comparative Transcriptomic Analysis of Embryonic Stem Cells (ESCs) across Mammalian Species. bioRxiv 2025.03.11.642582; doi: https://doi.org/10.1101/2025.03.11.642582 iScience
  2. Su,Y., Zhao, R., Fang, Y., Li, G., Jin, L., Liu, J., Zhu, J., Mishra, N., Kaback, D., Yee, S., Lei, C., Duan, J.*, Tian, X.*, Tang, T.*  (2025) Stem Cell. Bovine Formative Embryonic Stem Cells Capable of Three-Germ Layer and Extraembryonic Differentiation.
  3. Shi, M., Li, G., Araujo, H.M. et al. Sex-biased transcriptome in in vitro produced bovine early embryos. Cell Biosci 15, 123 (2025). https://doi.org/10.1186/s13578-025-01459-x
  4. Yu X., Harman R., Danev N., Li G., Fang Y., Van de Walle G., Duan J.E*. Under revision. Heat stress and recovery induce transcriptomic changes in lactogenic bovine mammary epithelial (MAC-T) cells. Physiological Genomics, 2025
  5. R.Biga*, J. E. Duan*, T E. Young*, J. R. Marks*, A. Bronikowski3*,L. P. Decena3*, E. C. Randolph1*, A. G. Pavuluri*, G. Li*, Y. Fang*, G. S. Wilkinson*, G. Singh#, N. T. Nigrin6#, E. N. Larschan6#, A. J. Lonski#, Nicole C. Riddle1^, and IISAGE Consortium (2024). Hallmarks of Aging: A User’s Guide for Comparative Biologists. 2024
  6. Li G*, Danev, N*, Duan, J*, Van de Walle G. (2024), Comparative transcriptomic analysis of bovine mesenchymal 1 stromal cells reveals tissue-source and species-specific differences. iScience
  7. Abdallah Abdelhady*, Jingzhi Zhang*, Chia-Kuan Wu, Yoke Lee Lee, Meihong Shi, Patrick L. Crane, Mariana Diel de Amorim, Ian R. Porter, Robert E. Thorne*, Jingyue (Ellie) Duan*, Soon Hon Cheong*. Cryopreservation impact the embryo transcriptomics and epigenomics. R1 in Scientific Reports. 2025
  8. Aguilera J., Duan J., Lee S., Ray M., Larschan E. The CLAMP GA-binding transcription factor regulates heat stress-induced transcriptional repression by associating with 3D loop anchors. bioRxiv 2025.10.08.561401; doi: https://doi.org/10.1101/2023.10.08.561401
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations 
  1. Duan et al., Bioinformatics Pipelines for the Integration Initiative: Sex, Aging, Genomics & Evolution (IISAGE)" Genomics and Epigenomics of Aging Workshop for the Plant and Animal Genome Conference (PAG)
  2. Randolph., Y. Fang et al Defining Ubiquitous and Sex-Biased Aging Biomarkers Using Transcriptional Data across Evolutionarily Diverse Model and Non-Model Organisms, Plant and Animal Genome Conference (PAG)
  3. Fang et al., Comparative Single-Cell Analysis of Epigenetic Regulator Dynamics during Early Gonadal Development, Plant and Animal Genome Conference (PAG)
  4. Li, G., Shi, M.,. et al. Building the Embryonic Isoform Atlas in Bovine Preimplantation Embryos, Plant and Animal Genome Conference (PAG)
  1. Abstracts
  1. Animal Epigenome Reprogramming: Developmental Dynamics and Emerging Tools

Yifei Fang 1,* , Guangsheng Li 1,* , Meihong Shi 1,* , Xingtan (Vera) Yu 1,* , Jingzhi Zhang 1,* , and Jingyue (Ellie) Duan 1,§, Advances in Animal Genomics (Editor: S. Mondal and R. L. Singh). 

  1. Miscellaneous publications (semi-technical/lay publications)

None

Nebraska - Publications

Montana - Publications

Arner, E.S.J., Schmidt, E.E., 2024. Unresolved questions regarding cellular cysteine sources and their possible relationships to ferroptosis. Advances in Cancer Research, 162, 1-44.

Borbényi-Galambos, K., Erdélyi, K., Ditrói, T., Jurányi, E.P., Szántó, N., Szatmári, R., Czikora, Á., Schmidt, E.E., Garai, D., Cserepes, M., Liszkay, G., Tóth, E., Tóvári, J., Nagy, P., 2025. Realigned Transsulfuration Drives BRAF V600E-Targeted Therapy Resistance in Melanoma. Cell Metabolism, in press.

Martí-Andrés, P., Finamor, I., Torres-Cuevas, I., Pérez, S., Rius-Pérez, S., Colino-Lage, H., Guerrero-Gómez, D., Morato, E., Marina, A., Michalska, P., León, R., Cheng, Q., Jurányi, E.P., Borbényi-Galambos, K., Millán, I., Nagy, P., Miranda-Vizuete, A., Schmidt, E.E., Martínez-Ruiz, A., Arnér, E.S., Sastre, J., 2024. TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. The EMBO Journal, 43, 2789-2812.

Schmidt, E.E., Miller, C.G., Juranyi, E.P., Austad, S.N., Ditroi, T., Seaford, Z.M., Yoon, S.J., Noyd, R.C., Kang, Y.P., Prigge, J.R., Ciskos, V., Kovari, D., DeNicola, G.M., Nagy, P., 2025. Cystine C-S bond cleavage fuels cysteine production under disulfide reductase deficiency. Nature Chemical Biology, in revision.

  1. Refereed articles/book chapters
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations 
  1. Abstracts 
  1. Miscellaneous publications (semi-technical/lay publications)

Missouri - Publications

Sponchiado M., W.Gu, D.M.C. Madrid, R.A. Carroll, K.M. Davenport, C.G. Lucas, R.M. Sullivan, K.D. Wells, J.P. Driver, W.C. Warren, R.S. Prather, R.D. Geisert. 2025. Programming of the endometrial immune milieu by Interferon Gamma during Early Pregnancy in Pigs. Biol Reprod. ioaf221. doi: 10.1093/biolre/ioaf22. Selected as the Editors’ Choice

Eitel E.K., M. Sponchiado, R.M. Sullivan, C.G. Lucas, B.K. Redel, P.R. Chen, K.D. Wells, R.S. Prather, W.C. Warren, and R.D. Geisert. 2025. Expression of Tripartite Motif Family-Like Protein 1 and 2 in Early Conceptus Development and Placentation During Pregnancy in the Pig. Reprod Fert 6:e240107. doi: 10.1530/RAF-24-0107. Selected as the Editors’ Choice

Speckhart SL, Pollock AB, Alward KJ, Farrell K, Oliver MA, Lee K, Biase FH, Ealy AD. The interleukin-6 signal transducer receptor subunit is required for optimal in vitro bovine embryo development. Biol Reprod. 2025 Jan 5:ioaf006. doi: 10.1093/biolre/ioaf006. Online ahead of print.

Noland RS, Redel BK, LaMartina MG, Prather RS, Chen PR. Transcriptional profiling reveals upregulation of p53 signaling in porcine embryos produced in vitro. Biol Reprod. 2025 Oct 14;113(4):777-786. doi: 10.1093/biolre/ioaf113.

Zhang J, Bai H, Kawahara M, Balboula AZ, Takahashi M. (2025). Functional role of circadian clock system in steroidogenesis and cell death pathways during corpus luteum regression in cattle. Cell Tissue Res. 402(1):65-78.

Chasi B, Andrade JP, Monteiro PLJ, Castro B, Moreno E, Anta E, Ali NN, Cole JB, Wiltbank, Peñagaricano F, Balboula AZ, Ortega MS MC. (2025). Association of fertility traits with markers of oocyte competence in dairy cattle, J Dairy Sci. 108(11):12814-12823. 

  1. Refereed articles/book chapters
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations
  1. Abstracts
  2. Soto-Moreno and A.Z. Balboula, Spindle-localized F-actin regulates the fidelity of meiotic spindle formation, Fertilization and Activation of Development Gordon Research Conference, Holderness, New Hampshire, USA, 27 July – 1 August 2025 (TALK) 
  1. Soto-Moreno and A.Z. Balboula, Spindle-localized F-actin regulates the fidelity of meiotic spindle formation, Fertilization and Activation of Development Gordon Research Seminar, Holderness, New Hampshire, USA, 26 July – 27 July 2025 (TALK)

Mangiavacchi P, Lee K, Redel BK. The Combination of CCL14 Chemokine and FLI During In Vitro Maturation Enhances In Vitro Development of Bovine Embryos. Society for the Study of Reproduction conference 2025, July 29 - August 1.

Nix J, Huang K, Kiesewetter E, Green C, Montgomery T, Samuel M, Lee K. Cryoprotective Treatment and Vitrification Stimulate the Oocyte Activation Cascade in Swine. Society for the Study of Reproduction conference 2025, July 29 - August 1.

Lee J, Yoon J, Miller L, Kim J, Redel B, Lee K. Canthaxanthin Reduces Polyspermy During In Vitro Fertilization in Pigs. Society for the Study of Reproduction conference 2025, July 29 – August 1.

Montgomery T, York M, Lee K. Identifying Lineage-Specific Transcripts in Different Embryonic Layers of Porcine Conceptuses During Peri-Implantation Development. Society for the Study of Reproduction Conference 2025, July 29 - August 1.

Green C, Jaworski A, Lee K, Prather RS, Redel B. Transcriptional profiling of cumulus cells from FGF, LIF, and IGF1 matured oocytes identifies junctional and structural pathways as key components in oocyte maturation. IETS Jan 18 – 22. 2025.

Kim J, Yoon J, Chen J, Lee H, Redel B, Prather RS, Lee K. Enhancing specificity of gene editing outcomes by using Cas9 variants in swine embryos. IETS Jan 18 – 22. 2025.

Yoon J, Mony S, Kiesewetter E, Sullivan R, Kim J, Redel B, Uh K, Prather RS, Lee K. Effects of stem cell factor on the in vitro maturation of porcine oocytes and subsequent development following in vitro fertilization. IETS Jan 18 – 22. 2025.

Montgomery T, Uh K, Prather RS, Lee K. Identifying PRDM family members potentially involved in epigenetic reprogramming after fertilization. IETS Jan 18 – 22. 2025.

  1. Miscellaneous publications (semi-technical/lay publications)

Mississippi - Publications

  1. Refereed articles/book chapters 
  • Dlamini NH, Bridi A, da Silveira JC, Feugang JM. Unlocking Gamete Quality Through Extracellular Vesicles: Emerging Perspectives. Biology. Feb 13;14(2):198. 2025 - https://doi.org/10.3390/biology14020198
  • Oladejo EO, Gruhot TR, Park S, Ishak GM, Mote BE, Liao SF, Feugang JM. Dietary Arginine Supplementation Modulates the Proteome of Boar Seminal Plasma. Animals. 2025 Feb 14;15(4):555. https://doi.org/10.3390/ani15040555
  • Dlamini NH, Santos-Rivera M, Vance-Kouba CK, Pechanova O, Pechan T, Feugang JM. Integrating Near-Infrared Spectroscopy and Proteomics for Semen Quality Biosensing. Biosensors. 2025 Jul 15;15(7):456. https://doi.org/10.3390/bios15070456 
  • Dlamini NH, Kameni SL, Feugang JM. Untargeted Metabolomic Identifies Potential Seasonal Biomarkers of Semen Quality in Duroc Boars. Biology. 2025 Aug 4;14(8):995. https://doi.org/10.3390/biology14080995
  • Kameni SL, Dlamini NH, Feugang JM. Differential Metabolomic Signatures in Boar Sperm with Varying Liquid Preservation Capacities at 17° C. Animals. 2025 Jul 22;15(15):2163. https://doi.org/10.3390/ani15152163
  • Beg MA, Ismail AO, Alaiya A, Khan FA, Hamoda TA, Sheikh IA, Sharma P, Baothman OM, Alkhzaim AH, Shinwari Z, Abuzinadah RF, Mohammed A, Assiri AM, Abuzenadah AM, Memili E, Feugang JM. Age-Associated Proteomic Changes in Human Spermatozoa. International Journal of Molecular Sciences. 2025 Jun 25;26(13):6099. https://doi.org/10.3390/ijms26136099
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations
  • Feugang JM, Othman OE, Nandolo W, Kugonza DR, Osei-Amponsah R. Prospect for Utilization of Modern Technologies for Small Ruminant and Pig Improvement in African Input Systems: Case Studies. In African Livestock Genetic Resources and Sustainable Breeding Strategies: Unlocking a Treasure Trove and Guide for Improved Productivity 2025 Nov 1 (pp. 1049-1068). Cham: Springer Nature Switzerland. 2025 - https://doi.org/10.1007/978-3-031-92076-9_24
  • Feugang JM, Osei-Amponsah R, Rege JE, Fantazi K, Tiambo CK, Shumbusho F, Houaga I, Harek D, Dlamini NH, Gaouar SB. Conservation and management of animal genetic resources in the context of African livestock production systems: The case for in situ and ex situ conservation. In African Livestock Genetic Resources and Sustainable Breeding Strategies: Unlocking a Treasure Trove and Guide for Improved Productivity 2025 Nov 1 (pp. 1071-1089). Cham: Springer Nature Switzerland. 2025 - https://doi.org/10.1007/978-3-031-92076-9_25
  • Ibeagha-Awemu EM, Peters SO, Bemji MN, Feugang JM, Osei-Amponsah R, Trocmé D, Mrode R. Role of modern technologies for sustainable genetic improvement of African livestock. In African Livestock Genetic Resources and Sustainable Breeding Strategies: Unlocking a Treasure Trove and Guide for Improved Productivity 2025 Nov 1 (pp. 851-908). Cham: Springer Nature Switzerland. 2025 - https://doi.org/10.1007/978-3-031-92076-9_20
  1. Abstracts
  • Mills M, Dlamini NH, Kameni SL, Maynard J, Brister T, Daous HE, Rajput S, Sidelinger DR, King HH, Feugang JM, Lemley CO. 41 Impact of oral melatonin supplementation on sperm quality and testicular artery hemodynamics in the bull. Journal of Animal Science. 2025 Jun;103(Supplement_2):136-. https://doi.org/10.1093/jas/skaf170.155
  • Dlamini NH, Kameni SL, Feugang JM. 31 Metabolomic analysis of boar seminal plasma identifies sperm-quality related metabolites. Journal of Animal Science. 2025 Jun;103(Supplement_2):139-. https://doi.org/10.1093/jas/skaf170.158
  • Kameni SL, Dlamini NH, Feugang JM. 87 Metabolomic insights into preserved boar semen: Differential profiles in high-survival and low-survival semen during liquid storage. Journal of Animal Science. 2025 Jun;103(Supplement_2):49-. https://doi.org/10.1093/jas/skaf170.061

Louisiana - Publications

  1. Refereed articles.

Brewer, A.*, Girka, E.*, Dalton, A., Gutierrez-Castillo, E., and K. R. Bondioli. 2025. Bovine oocyte vitrification and recovery with ethylene glycol and either propylene glycol or dimethyl sulfoxide. Theriogenology. 9:242:117439.

  1. Abstracts

Gutierrez-CastilloA, E. A. Hoang, F. Diaz, S. Dawn and K. Bondioli, R. 2026. Exploration of the cryoprotective properties of carboxylated poly-L-lysine for vitrification of IVM bovine oocytes. Reprod. Fert. Dev. 38:19.

 

Iowa - Publications

Illinois - Publications

  1. Refereed articles/book chapters

1). Wang, Geng, Janet E. Sorrells, Rishyashring R. Iyer, Jindou Shi, Carlos A. Renteria, Edita Aksamitiene, Eric J. Chaney, Jennifer Mitchell, R.A.C. “Chanaka” Rabel, Derek J. Milner, Matthew B. Wheeler, Martha U. Gillette, Stephen A. Boppart, Haohua Tu. Label-free super-multiplex multiphoton imaging microscopy. Proceedings Volume PC13311, Optical Biopsy XXIII: Toward Real-Time Spectroscopic Imaging and Diagnosis; PC133110Q (2025) https://doi.org/10.1117/12.3040967
Event: BiOS, 2025, San Francisco, California, United States.

2). Womack , S.A., Bethke, E., King, W.P., Milner, D.J., Rubessa, M., Marchioretto, P.V., Shipley, C.F., Wheeler, M.B., Development of a Porcine Model for the Testing of the RapidVent Emergency Ventilator for the Treatment of COVID-19. J Reg Med Biol Res. 2025;6(2):1-9. https://doi.org/10.46889/JRMBR.2025.6204

3). Marchioretto, Paula V., Sandra L. Rodriguez-Zas, Brad R. Lindsey, and Matthew B. Wheeler. Effect of Follicle Ablation Before Ovum Pick-Up in Girolando Cattle Journal of Scientific Agriculture 2025, 9: 1-5. https://doi.org/10.25081/jsa.2025.v9.9602

4). Bangert, E. A.  and M. B. Wheeler. Assisted Reproductive Technologies and Genetic Improvement Strategies for Enhancing Livestock Production and Food Security in Tropical East Africa: A Review. Journal of Scientific Agriculture 2025, 9: 1-15 https://doi.org/10.25081/jsa.2025.v9.9599

5). Bionaz M., Jensen, T.W., Monaco, E., Maki, A.J., Rabel, R. A. C., Wheeler, M. B. In-vitro Osteogenic Capacity and In-vivo Bone Healing of Porcine Freshly Isolated Adipose-Derived Stem Cells (ASC), CS34+ ASC and Bone Marrow-Derived Stem Cells. J Reg Med Biol Res. 2025;6(3):1-18. https://doi.org/10.46889/JRMBR.2025.6306

6). Kanzawa, S., O’Meara, E., Funnell, B. J.,  Bangert, E.A., Garrett, E.F., Cardoso, F. C., Braz, C. U.,  Wheeler, M. B. The Effects of Heat Stress on the Population of the Ovarian Follicles and in vitro Embryo Production in Dairy Cows. Theriogenology (published online December 23, 2025) Volume 253, 15 March 2026, 117790, https://doi.org/10.1016/j.theriogenology.2025.117790

7). Gweniviere Capron, Parinaz Fathi, Tor Wolf Jensen, Derek J. Milner, André J. van der Vlies, Regan L. Moody, Elizabeth A. Bangert, Blair Rowitz, Dipanjan Pan, and Matthew B. Wheeler. 3D-Printed Gastrointestinal Stents: In Vivo Evaluation In a Swine Small Bowel Perforation Model. Advanced Healthcare Materials (2025): e05169. https://doi.org/10.1002/adhm.202505169 First published: 26 December 2025.

  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

N/A

  1. Abstracts

1).  De Oliveira AT, Bangert B, Rubessa M, Milner D, Okonkwo J, Ponnuraj N, Sivaguru M, Wheeler MB. (2026) Mapping transcript distribution in in vitro bovine embryos using spatial transcriptomics. Reproduction Fertility and Development 38, RDv38n1Ab189. https://doi.org/10.1071/RDv38n1Ab189

2).  De Oliveira AT, Rubessa M, Ulanov A, Scott B, Wheeler MB. (2026) Forever chemical: industrial contaminant detection across US bovine embryos. Reproduction Fertility and Development 38, RDv38n1Ab90. https://doi.org/10.1071/RDv38n1Ab90

3).  De Oliveira AT, Rubessa M, Milner D, Berbel AM, Ponnuraj N, Sivaguru M, Wheeler MB. (2026) Spatial transcriptomics of in vivo–produced bovine embryos: transcript density in three distinct embryonic cell lineages. Reproduction Fertility and Development 38, RDv38n1Ab190. https://doi.org/10.1071/RDv38n1Ab190

4).  De Oliveria AT, Braz C, Zimmerman L, Stewart K, Scott B, Wheeler MB. (2026) Comparative analysis of semen separation methods for in vitro fertilization in goats. Reproduction Fertility and Development 38, RDv38n1Ab104. https://doi.org/10.1071/RDv38n1Ab104

5).  Bangert EA, Lindsey BR, Rubessa M, Newman JK, Naves LM, Marchioretto PV, Wheeler MB. (2026) Improving bovine embryo transfer outcomes through precise alignment of embryo development with recipient estrous cycles. Reproduction Fertility and Development 38, RDv38n1Ab94. https://doi.org/10.1071/RDv38n1Ab94

6).  Zimmerman L, De Oliveira AT, Rubessa M, Ulanov A, Scott B, Wheeler MB. (2026) Forever chemical: industrial contaminant detection between in vitro- and in vivo-produced embryos. Reproduction Fertility and Development 38, RDv38n1Ab91. https://doi.org/10.1071/RDv38n1Ab91

7).  Nasser L, Bangert EA, Braz CU, Jimenez R, Torres ME, Wheeler MB. (2026) Use of GnRH versus P4 as an exogenous hormonal support for corpus lutea to improve embryo transfer pregnancy rates in crossbred recipients under a tropical environment. Reproduction Fertility and Development 38, RDv38n1Ab8. https://doi.org/10.1071/RDv38n1Ab8

8).  Wheeler, M.B., ASAS Randel Lecture: Bovine Embryo Evaluation—From Optics to Omics: My Journey, June 2025, Journal of Animal Science 103(Supplement 2):48-49, DOI: 10.1093/jas/skaf170.060 

  1. Miscellaneous publications (semi-technical/lay publications)

US Patent Number. 12,067,712B2 M.B. Wheeler et al. Date of Patent Issued :Aug. 20, 2025. Title: Phase Imaging with Computational Specificity” Filed: February 18, 2020. Application: Assessment for viability of sperm and embryos via artificial intelligence neural networks.

Florida - Publications

  1. Referred articles
  1. Jiang, Z. (2025). Blastoids for modeling early embryonic development: application to domestic livestock. Annual Review of Animal Biosciences. https://doi.org/10.1146/annurev-animal-111523-102012
  2. Jiang, Z. (2025). Molecular factors driving the development of bovine embryos and embryo-like structures. Animal Reproduction, 22(3). https://doi.org/10.1590/1984-3143-AR2025-0056
  3. Ming H, Iyyappan R, Kakavand K, Dvoran M, Susor A, Jiang Z#. Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development. Nucleic Acids Research, 53(18). https://doi.org/10.1093/nar/gkaf956
  4. Ming, H., Scatolin, G. N., Ojeda-Rojas, O. A., & Jiang, Z. (. (2025). Establishment of bovine extraembryonic endoderm stem cells enables efficient blastoid formation. Cell Reports, 44(6), 115707. https://doi.org/10.1016/J.CELREP.2025.115707
  5. Zhu, L., Ming, H., Scatolin, G. N., Xiao, A., & Jiang, Z. (. (2025). METTL7A improves bovine IVF embryo competence by attenuating oxidative stress. Biology of Reproduction, 112(4), 628-639. https://doi.org/10.1093/BIOLRE/IOAF018.
  6. Siddique MS, Anand S, Gauthier MPL, Brant JO, Kladde MP, Bhandari RK, Daigneault BW. Tributyltin chloride alters the structural, genomic, and epigenomic integrity of postejaculatory mammalian sperm. Epigenetics. 2025. Ahead of Press: http://dx.doi.org/10.1080/15592294.2025.2552129.
  7. Mallory J. Llewellyn, Muhammad S. Siddique, Emma Ivantsova, Bradford Daigneault, Tracie R. Baker, Christopher J. Martyniuk. Lead as a reproductive endocrine disruptor in aquatic species and agricultural livestock. Pollutants. 2025. 5(3), 28; https://doi.org/10.3390/pollutants5030028
  8. Losano JDA, Ren J, Yue F, Daigneault BW. Implementation of high-resolution respirometry for modeling bull fertility. J Animal Sci. 2025 Jan 4:103:skaf209. doi: 10.1093/jas/skaf209.
  9. Mauro Venturini, Joao Agostini de Losano, Daniella C Heredia, Maria C Lopez Duarte, Maura McGraw, Sohail Muhammad, Jillian Guertin, Nicolas Dilorenzo, Bradford W Daigneault, Angela M Gonella-Diaza. Association between residual feed intake and reproductive-related traits in young bulls. J Animal Sci. 2025 https://doi.org/10.1093/jas/skaf055.
  10. Guertin JE, Losano JDA, Salazar S, Callaham J, Daigneault, BW. Prolonged maintenance of cooled stallion semen by optimization of storage conditions. Journal of Equine Veterinary Sciences. 2025 Jan;144:105243. doi: 10.1016/j.jevs.2024.105243.
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

    Abstracts

  1. Siddique MS, Anand S, Bhandari RK, Daigneault BW. Recreational levels of THC exposure from cannabis elicits structural, genomic and novel epigenomic changes to mature sperm that impact embryo development. Society for the Study of Reproduction 2025.
  2. Siddique MS, Ananad S, Gauthier M, Martyniuk M, Kladde M, Bhandari R, Daigneault BW. Mature Mammalian Sperm are Suseptible to Novel Epigenetic Modifications and Structural Damage from Environmental Toxins. Society for the Stud of Reproduction 2025.
  1. Miscellaneous

Invited Speaker for the Dairy Podcast Show - Daigneault, B. W. (2025). Improving Reproductive Performance - Bull Fertility Evaluation. The Dairy Podcast Show. https://youtu.be/U4-23_HUxrA?si=4NWzVPMQEwZt1Bdx

Connecticut - Publications

  1. Refereed articles/book chapters: (*=correspondence)

1) Zhao R, Liu J, Bakhtari A, Shen J, Fayad-Costa A, Wang B, and Tian* X. 2025. Evidence against the role of toll-like receptors 7 and 8 in sex selection in mice, cattle, and humans. iScience 28, 113164; https://doi.org/10.1016/j.isci.2025.113164

2) Fang Y, Su Y, Meisel R, Walters JR, Gamble T, Randolph EC, Yu X, Shi  M, Li G, Zhang J, IISAGE Consortium, Tian* XC, Tang* Y, Duan JE. 2026. Comparative transcriptomic analysis of embryonic stem cells across mammalian species.  iScience 29, 114542; https://doi.org/10.1016/j.isci.2025.114542

3) Ranjitkar S, Shiri M, Sun J, Tian* X. 2025. Poly(A)-Selected Intergenic Transcripts in In Vivo Developed Bovine Oocytes and Pre-Implantation Embryos. Mol Reprod Dev 2025 Feb;92(2):e70017; https://doi.org/10.1002/mrd.70017

4) Su Y, Zhao R, Fang Y,Renxiu M, Li G, Jin L, Liu J, Yang Z, Li N,Zhu J, Mishra N, Kaback D, Yee SP, Luo Y, Wan W, Zhao Y, Wang X, Han S, Xiao P, Lei C, Duan* JE, Tang* Y, Tian* XC. 2026. Bovine formative embryonic stem cell plasticity in embryonic and extraembryonic differentiation. Stem Cells. Jan 8;44(1):sxaf068. doi: 10.1093/stmcls/sxaf068.

  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations: N/A
  1. Abstracts

1) Bielski K, Zhao R, Bakhtari A, Liu J, Guan Z, Tian X. 2026. Presented at the IETS Annual Conference in Panama City, Panama (abstract #188). Multipolar Mitosis as a Self-Correction Mechanism for Polyploidy in Pre-implantation Mouse Embryos

  1. Miscellaneous publications (semi-technical/lay publications): N/A

Colorado - Publications

  1. Refereed articles/book chapters

Publication (Winger lab)

Omar AK, Puma LCL, Risk BD, Witt AC, Izon CS, Whitcomb LA, Kareng DJ, Winger QA, Bouma GJ, Chicco AJ. Validation of an ovine model for studying impacts of prenatal docosahexaenoic acid supplementation on fetal tissue membrane composition and metabolism. J Nutr Biochem. 2025 Oct 4;148:110137. doi: 10.1016

Publication (Barfield lab)

Guzeloglu, A., Bishop, J. V., Georges, H. M., Braun, H. E., Menjivar, N., Marquez, M. A., Hansen, T. R., & Barfield, J. P. (2025). Identification of transcriptome markers associated with in vitro bovine embryo quality in single embryos and biopsies†. Biology of reproduction, ioaf260. Advance online publication. https://doi.org/10.1093/biolre/ioaf260

Lugo, E. A., Graham, J. K., Gillis, J. D., & Barfield, J. P. (2026). Investigating the plasma membrane composition and osmotic tolerance of domestic felid (Felis catus) spermatozoa as a model for non-domestic felids. Animal reproduction science, 285, 108076. https://doi.org/10.1016/j.anireprosci.2025.108076

Publication (Tesfaye lab)

Nico G Menjivar, Ahmed Gad, Riley E Thompson, Mindy A Meyers, Soham Ghosh, Fiona K Hollinshead, Dawit Tesfaye 2025. Organoids simulating the bovine oviduct mediate the embryo-maternal interface via extracellular vesicle-transmitted signaling, Human Reproduction Open, hoaf076, https://doi.org/10.1093/hropen/hoaf076

Heather M Rogers, Ahmed Gad, Gentry K Cork, Nico G Menjivar, William B Schoolcraft, Dawit Tesfaye, Ye Yuan 2025. Age-related integrative transcriptomic profiling of human granulosa cells reveals mRNA-microRNA regulatory network associated with key ovulation dynamics. Biology of Reproduction, f034, https://doi.org/10.1093/biolre/ioaf034

E Held-Hoelker, J Kurzella, C Benedetti, H Habermann, F Rings, E Tholen, D Tesfaye, M Hoelker. 2025. The mitochondrial bioenergetic profile in response to L-Carnitine as a clue for vitality of in vitro produced bovine embryos cultured under serum enriched conditions. Theriogenology, Volume 251,117746, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2025.117746

  1. Rahimi, M. Schreiber, H. Habermann, L. Haake, D. Miskel, D. Tesfaye, E. Held-Hoelker, B. Brenig, C. Benedetti, M. Hoelker. 2025. Efficient genome editing of bovine in vitro derived zygotes via Cas9 RNP-electroporation using extended-stored bovine ovaries, Theriogenology, Volume 250, 2026, 117703, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2025.117703.

Luca Angi Souza, Nico G. Menjivar, Ahmed Gad, Paulo Henrique Groppo Rodrigues, Leticia Rabello da Silva Sousa, Paola Maria da Silva Rosa, Alessandra Bridi, Dawit Tesfaye, Juliano Coelho da Silveira 2026: Small extracellular vesicles from follicular fluid as transport carriers of LHR: possible mediators of follicle growth and dominance acquisition in bovine reproduction. Anim Reprod. 2026;23(1):e20250051 | https://doi.org/10.1590/1984-3143-AR2025-0051

Dessie Salilew-Wondim, Ernst Tholen, Christine Große-Brinkhaus, Eva Held-Hoelker, Dennis Miskel, Franca Rings, Karl Schellander, Urban Besenfelder, Vitezslav Havlicek, Dawit Tesfaye & Michael Hoelker 2025. Sexually dimorphic gene expression responses of bovine embryos to the maternal microenvironment on day 13 of gestation. BMC Genomics 26, 372. https://doi.org/10.1186/s12864-025-11570-5

Dennis Miskel, Jessica Kurzella, Franca Rings, Ernst Tholen, Dawit Tesfaye, Karl Schellander, Dessie Salilew-Wondim, Eva Held-Hoelker, Christine Große-Brinkhaus, Michael Hoelker 2025. Functional COPA is indispensable for early embryo development beyond major genome activation in bovines. Theriogenology, 241 (117415). https://doi.org/10.1016/j.theriogenology.2025.117415

  1. Schreiber, C. Benedetti, M. Rahimi, H. Habermann, F. Rings, E. Tholen, D. Tesfaye, M. Hoelker, E. Held-Hoelker. 2025. Tauroursodeoxycholic acid (TUDCA) improves cryo-resilience and lowers energy turnover via affecting the mitochondrial bioenergetic profile in bovine embryos, Theriogenology, Volume 249, 2026, 117632, ISSN 0093-691X, https://doi.org/10.1016/j.theriogenology.2025.117632.

Eva Held-Hoelker, Lientje Sophie Haake, Jessica Kurzella, Maibritt Schreiber, Christina Dauben, Dessie Salilew-Wondim, Nasser Ghanem, Franca Rings, Christine Große-Brinkhaus, Ernst Tholen, Dawit Tesfaye, Michael Hoelker (2025). Heat stress during maturation of bovine oocytes profoundly impacts the mitochondrial bioenergetic profile and causes endoplasmic reticulum stress in subsequent blastocysts, Biology of Reproduction, ioaf070, https://doi.org/10.1093/biolre/ioaf070

Rahman, M.B., Held-Hoelker, E., Saeed-Zidane, M. Rings, Franca, Salilew-Wondim, Dessie, Tesfaye, Dawit, Gad, Ahmed, Gebremedhn, Samuel, Tholen, Ernst, Schellander, Karl, Große-Brinkhaus, Christine, Hoelker, Michael. 2025. Comparative transcriptome analysis of bovine blastocysts reveals specific effects of the oocyte source and the environments during maturation and early embryo development. BMC Genomics 26, 673 (2025). https://doi.org/10.1186/s12864-025-11848-8

  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertation 

Nico G. Menjivar​, Camila Azzolin de Souza​, Ahmed Gad, Luca A. Souza, Samuel Gebremedhn, Juliano C. da Silveira, Dawit Tesfaye. 2025. Strategies for Loading miRNA Cargo into Extracellular Vesicles. In Neuro-methods Series

  1. Abstracts and Talks

Invited talks by PI (Barfield)

Barfield JP. Embryo Grading Webinar, Universidad Cientifica del Sur, Peru, March 2025

Barfield JP. Practical Considerations for Embryo Freezing, American Embryo Transfer Association, Reno, NV, Oct 2025

California - Publications

  • Denicol AC, Botigelli RC. Dissecting the development of the early lineages and primordial germ cells in the bovine embryo. Anim Reprod. 2025 Oct 3;22(3):e20250098. doi: 10.1590/1984-3143-AR2025-0098. PMID: 41059287
  • Candelaria JI, Botigelli RC, Guiltinan C, Shikanov A, Denicol AC. Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles. J Assist Reprod Genet. 2025 May;42(5):1509-1523. doi: 10.1007/s10815-025-03497-3. Epub 2025 May 20. PMID: 40392485
  • Guiltinan C, Botigelli RC, Candelaria JI, Smith JM, Arcanjo RB, Denicol AC. Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics. Biol Open. 2025 Mar 15;14(3):BIO061819. doi: 10.1242/bio.061819. Epub 2025 Mar 7. PMID: 39957479
  1. Refereed articles/book chapters
  1. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations 
  1. Abstracts

Transition of embryonic stem cells to low oxygen conditions reveals changes associated with energetic metabolism without altering the pluripotency core. R. C. Botigelli, R. Braz-Arcanjo, C. Guiltinan, J. Smith and A. C. Denicol. [Conference abstract]. Reproduction, Fertility and Development 38, RDv38n1Ab1. doi:10.1071/RDv38n1Ab1

Embryonic genome transcription in cattle occurs as early as the 4-cell stage and involves mRNA processing factors, epigenetic regulators, and conserved transcription factors. M. D. Altman, A. C. Denicol, R. M. Schultz and P. J. Ross. [Conference abstract]. Reproduction, Fertility and Development 38, RDv38n1Ab1. doi:10.1071/RDv38n1Ab1

Evaluation of mechanical isolation techniques for efficient harvesting of bovine preantral follicles

  1. A. Hyde, A. N. P. Morais, A. L. Conceição-Santos, L. Engelman, M. O. Gastal, B. Graceli, A. C. Denicol and E. L. Gastal [Conference abstract]. Reproduction, Fertility and Development 38, RDv38n1Ab1. doi:10.1071/RDv38n1Ab1

McDonnell SP, McKissick EE, Denicol AC. The Role of Connexin 37 in Early Antral Bovine Folliculogenesis. Abstract presented at the 2025 Annual Meeting of the Society for the Study of Reproduction, Washington DC, USA. 

  1. Miscellaneous publications (semi-technical/lay publications)

Arkansas - Publications

 

 

 

 

 

 

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