SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

In Attendance - Virtually: 1. Caleb Lemley, Mississippi State University 2. David Grieger, Kansas State University 3. Ky Pohler, Texas A&M University 4. John Hall, University of Idaho 5. Ryan Ashley, New Mexico State University 6. Karl Kerns, Iowa State University 7. Kimberly Davenport, Washington State University 8. Thiago Martins, University of Missouri In Attendance – In person 1. Kristen Govoni, University of Connecticut 2. Jessica Nora Drum, South Dakota State University 3. Manuel Alexander Vasquez-Hidalgo, South Dakota State University 4. Rafael Domingues, The Ohio State Universit 5. Rick McCosh, Colorado State University 6. Katherine Hallora, University of Kentucky 7. Sarah McCoski, Montana State University 8. Becky Poole, Texas A&M University 9. Jeremy Block, University of Wyoming 10. Brenda Alexander, University of Wyoming 11. Sofia Ortega, University of Wisconsin 12. Corrine Monaco (Post Doc), University of Nebraska 13. Mario Binelli, University of Florida 14. Phill Cardoso, University of Illinois

Minutes-2025-W4112-Multistate-Meeting-Storrs-CT.docx

Accomplishments

Outputs from the group during this reporting period:

  1. 100 peer-reviewed published papers.
  2. 75 Published Abstracts.
  3. 5 Thesis/Dissertations.
  4. 20 Invited lectures.
  5. 4 book chapters.

Activities

Objective 1: Elucidate fundamental cellular, physiological, endocrine, and behavioral mechanisms that regulate gamete development and quality and enhance the management of reproductive function leading to the development of translational reproductive biotechnologies.

  1. Oral melatonin supplementation during fall-winter improved sperm motility in bulls without affecting testicular blood flow. (Mississippi)
  2. PRAMEY enhances sperm-oocyte binding and modulates epigenetic marks in early bovine embryos, suggesting a role in fertilization and early development. (Pennsylvania)
  3. Disruption of PGRMC and lipid metabolism in granulosa and luteal cells accelerates ovarian aging by depleting the primordial follicle pool. (Wyoming)
  4. Non-cycling heifers show altered granulosa cell responses to FSH, contributing to delayed puberty; FSH treatment may partially rescue follicular development. (Nebraska)
  5. Phthalate exposure (DBP, BBP, DEHP) disrupts ovarian IGF1 signaling and fatty acid metabolism, impairing folliculogenesis and ovarian reserve. (Arizona)
  6. Heat stress alters transcriptomic responses in bovine preantral follicles and disrupts gap junction communication, affecting cumulus-oocyte complex function. (California)
  7. MACS-based enrichment of ovine gonadotropes using anti-KCNMA1 antibody improves purity and retains estradiol responsiveness, enabling better study of pituitary function. (Colorado)
  8. Developed CRISPR/SaCas9 tools for ESR1 gene deletion and neurosurgical methods to target the nucleus of the solitary tract, advancing neuroendocrine research. (Colorado)
  9. Larger follicles improve oocyte cytoplasmic maturation (e.g., mitochondrial activity), though blastocyst rates remain unchanged; lipid accumulation is reduced in embryos. (Wisconsin)
  10. Aggresome accumulation in sperm is linked to oxidative stress and impaired embryo development, highlighting a paternal contribution to early embryonic health. (Wisconsin)

Objective 2: Identify impacts of reproductive management, animal management, and stress on follicle recruitment, ovulation, corpus luteum function, and pregnancy.

  1. Extending CIDR duration in CO-Synch increased dominant follicle size but did not improve estrus response or pregnancy rates. (Missouri)
  2. Estrus expression timing and CL age on day 14 may reduce synchronization success in the 7&7 Co-Synch protocol. (South Dakota)
  3. Vaginitis during 14-day implant protocols is common but does not affect pregnancy outcomes. (South Dakota)
  4. Melatonin supplementation in nutrient-restricted dams alters fetal epigenetic and transcriptomic profiles. (Mississippi)
  5. Flaxseed oil (rich in omega-3s) may help advance puberty by improving the endocrine environment in beef heifers. (South Dakota)
  6. ω-6 fatty acid supplementation around AI did not improve progesterone levels or conception rates. (Idaho)
  7. Trace mineral injections during gestation may enhance birth weight and male reproductive traits, especially in multiparous cows. (Montana)
  8. Shearing ewes at day 50 of gestation increases uterine blood flow, fetal size, and may improve birthweight in multiples. (South Dakota)
  9. Extended darkness in early gestation is being studied for its effects on umbilical blood flow and placental development. (South Dakota)
  10. Creatine supplementation in gilts had limited effects on fetal growth but may influence placental gene expression. (Texas)
  11. Vaginal microbiota shifts between AI and maternal recognition of pregnancy, suggesting a role in early pregnancy establishment. (Texas)
  12. Bull reproductive organs harbor distinct microbial communities, indicating niche-specific microbiomes. (Wyoming)
  13. Open cows can be identified with 95–100% accuracy as early as days 16–17 post-AI. (Colorado)
  14. Estradiol and IL1B production in swine increases with embryo size; female embryos secrete less estradiol, suggesting sexual dimorphism. (Pennsylvania)
  15. Heat stress may impair early follicular recruitment post-GnRH, though not affecting follicle count at day 7. (Illinois)
  16. Rumen-protected methionine alters lipid profiles in embryos and endometrium during early lactation. (Illinois)
  17. Dominance hierarchy in rams does not significantly affect reproductive success in multi-sire systems. (Wyoming)
  18. Thyroid hormone activity in the brain’s reward pathway may influence mating and feeding behaviors. (Wyoming)
  19. BVDV infection in late gestation reduces offspring performance, potentially impacting herd profitability. (Colorado)
  20. Hands-on reproductive training significantly improves rancher skills and yields \$1,000–\$5,000 ROI per ranch annually. (Idaho)

Objective 3: Determine mechanisms regulating normal embryo development, pregnancy establishment, and maintenance by exploring maternal and paternal factors, including genomics, immune responses, fetal programming, and conceptus/uterine signaling.

  1. Studies identified distinct cell populations (e.g., trophoblasts, mesenchyme, immune cells) and gene regulatory networks in bovine and ovine placentas, with species-specific clusters and conserved expression patterns. (Washington)
  2. Genetic variation in transcription factor binding sites may disrupt placental gene expression and affect fertility. (Washington)
  3. Poor maternal diet and nutrient restriction during gestation alter DNA methylation, placental function, and fetal metabolic activity, with sex-specific effects on fetal development. (Connecticut, Montana)
  4. Betaine supplementation and flaxseed oil influence fetal glucose, insulin, and progesterone concentrations, though not always improving reproductive outcomes. (Connecticut, South Dakota)
  5. Gestational melatonin supplementation modulates the transcriptome related to conceptus growth in a sex-dependent manner, without altering dam behavior. (Mississippi)
  6. PGRMC1 overexpression impairs fertility and fetal growth, while its absence alters amino acid and lipid metabolism, highlighting its role in uterine homeostasis. (Wyoming)
  7. ISG15 expression and its conjugation pathway increase at the implantation site, indicating a role in maternal recognition of pregnancy. (Wyoming)
  8. P4 supplementation promotes earlier embryonic attachment and PAG detection, though it does not affect IFNT secretion. (Ohio)
  9. Sire conception rate correlates with pregnancy outcomes but not with embryo development or loss. (Ohio)
  10. New molecular tools allow mRNA isolation from single morula biopsies, aiding embryo diagnostics. (Colorado)
  11. Vaginal and uterine microbiomes shift with synchronization protocols and fetal sex, while fetal immune cell populations are altered in FGR sheep, suggesting immune dysfunction. (South Dakota, Arizona)
  12. Efforts are ongoing to develop DNA methylation signatures for Large Offspring Syndrome, and SNPs associated with fertility have been identified. (Missouri, Colorado)
  13. Genomic variants on BTA11 and BTA25 in Jersey bulls impair fertilization and blastocyst formation, with distinct mechanisms affecting male fertility. (Wisconsin)
  14. PAG7 is essential for endometrial remodeling during placentation but not during early embryo development. (Wisconsin)
  15. Amino acid supplementation enhances trophectoderm growth and gene expression, offering a strategy to reduce early pregnancy loss. (Wisconsin)
  16. CXCL12/CXCR4 axis manipulation affects vascular and immune signaling at the fetal–maternal interface. (New Mexico)
  17. Endometrial memory of hormonal stimuli affects cell behavior and fertility. (Florida)
  18. BRaf–STAT3–Bcl2 signaling is critical for fetal vascular stem cell survival. (Arizona)
  19. Endotoxin exposure causes hematological changes that may reduce reproductive efficiency. (New Mexico)

Impacts

  1. Research into estrous synchronization and hormonal regulation has yielded significant insights into improving reproductive efficiency in livestock. Extending CIDR treatment duration has been shown to influence dominant follicular growth, estrous response, and fertility, offering potential refinements to synchronization protocols. The 7&7 Synchronization protocol has been better characterized, enhancing our understanding of estrus distribution and hormonal responses. Additionally, studies on estradiol's influence on pituitary transcriptional networks provide valuable information for optimizing ovulation timing. The use of FSH-β knockout sheep models has deepened our understanding of FSH signaling in ovarian reserve establishment and follicular development, with implications for both cattle and human reproductive health.
  2. Nutritional supplementation plays a critical role in reproductive outcomes. Myo-inositol and melatonin have shown promise in enhancing fertility, placental gene expression, and sperm quality. However, ω-6 fatty acid supplementation did not yield reproductive benefits and was associated with reduced progesterone levels, suggesting complex interactions with basal diets. Creatine supplementation influenced fetal organ weights and muscle gene expression, indicating potential developmental effects. Furthermore, essential amino acid availability was found to directly impact trophectoderm growth and early embryonic development, highlighting the importance of maternal nutrition in pregnancy retention and embryo viability.
  3. Advancements in ART have been supported by research into IGF signaling in conceptuses, which informs improvements in in vitro production (IVP) methodologies. Genetic studies have linked sire genetics and embryo phenotypes to fertility outcomes, enabling better selection strategies. Biomarkers and SNPs have been identified to predict embryo development success, and AI-driven estrus detection has improved embryo transfer timing. These innovations enhance the efficiency and success rates of ART, contributing to more sustainable breeding practices.
  4. Reproductive microbiomes have emerged as a key factor in fertility. Studies have characterized unique microbial populations in vaginal and testicular environments, with implications for reproductive health. Alterations in microbiota have been linked to fertility outcomes in cattle. The ISG15 protein has been validated as a pregnancy marker through its conjugation in uterine tissue. Additionally, immune cell transcriptomics offer potential for predicting infection risk and postnatal performance, paving the way for new diagnostics and therapeutic strategies.
  5. Our research has identified critical genes such as PRAME/PRAMEY and PGRMC1/2 that influence spermatogenesis, embryo development, and reproductive lifespan. Trophoblast gene networks have been shown to play essential roles in pregnancy establishment, and PAGs have been studied for their involvement in embryo–maternal communication. These findings enhance our understanding of the molecular underpinnings of fertility and provide targets for improving reproductive outcomes.
  6. Environmental stressors, particularly heat stress, have been shown to impair oocyte quality and embryo yield, despite increased follicle numbers. Exposure to endocrine disruptors like atrazine has been linked to reduced dopamine synthesis in male offspring, affecting behavior and productivity. Neuroscientific studies are exploring how stress impacts reproductive efficiency, with the goal of developing strategies to mitigate these effects and improve resilience in livestock.
  7. Innovative diagnostic tools such as the Open Cow Test (OCT) enable early pregnancy detection and management of open cows, improving herd productivity. DNA-based diagnostics and SNP markers are being developed to identify subfertile cows and predict fertility. Biomarkers for embryo mortality and immune cell transcriptomics are also being explored to enhance reproductive management and reduce economic losses due to subfertility.
  8. Research into fetal and placental development has revealed sex-specific effects of maternal nutrition on organ development and PAG release. Uteroplacental nutrient availability has been linked to conceptus development and survival. These findings underscore the importance of maternal environment in shaping fetal outcomes and inform strategies to improve pregnancy success and offspring viability.
  9. Education and technology transfer efforts have demonstrated the value of hands-on training in promoting the adoption of new reproductive technologies. Involvement of undergraduate and graduate students in research projects has fostered knowledge dissemination and capacity building. These initiatives support the long-term integration of scientific advancements into livestock production systems.

Publications

  1. Altman M.D., Mathews A.T., Rabaglino M.B., Hovey R.C. & Denicol A.C. (2025) Canonical prolactin signaling and global mRNA expression in the skin of Holstein heifers carrying the SLICK1 allele of the prolactin receptor gene. Journal of Dairy Science.
  2. Amirrad F., La V., Ohadi S., Albotaif M., Webster S., Pru J.K., Shamloo K., Mohieldin A.M. & Nauli S.M. (2025) PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in mice. Nature Communications 16, 2422.
  3. Andrade J.P.N., Domingues R.R., Carvalho B.P., Gomez-Leon V., Prata A.B., Sartori R. & Wiltbank M.C. (2024) Optimizing ReBreed21 I: Evaluation of endocrine and ovarian dynamics in non-bred Bos indicus heifers. Theriogenology 220, 77-83.
  4. Andrade J.P.N., Gomez-León V.E., Madureira G., Sartori L.C., Grillo G.F., Domingues R.R., Fosado M., Sala R.V. & Wiltbank M.C. (2025) ReBreed21-ET: Evaluation of a rapid resynchronization program that allows timed embryo transfer every 21 days. Theriogenology 235, 145-51.
  5. Andrews T.N. (2021) The Interactions of Change in Nutrition Prior to and After Artificial Insemination on Plasma Metabolites, Steroid Hormones, and Uterine Histotroph in Beef Heifers.
  6. Bazer F.W. & Johnson G.A. (2024) Early embryonic development in agriculturally important species. Animals 14, 1882.
  7. Bazer F.W., Wu G. & Johnson G.A. (2024) Fructose metabolism is unregulated in cancers and placentae. Experimental Biology and Medicine 249, 10200.
  8. Beiki H., Murdoch B.M., Park C.A., Kern C., Kontechy D., Becker G., Rincon G., Jiang H., Zhou H. & Thorne J. (2024) Enhanced bovine genome annotation through integration of transcriptomics and epi-transcriptomics datasets facilitates genomic biology. Gigascience 13, giae019.
  9. Binelli M., Lopez-Duarte M.C., Gonella-Diaza A., Silva F.A., Pugliesi G., Martins T. & Rocha C.C. (2025) Effectors and predictors of conceptus survival in cattle: What is next? Domestic Animal Endocrinology 92, 106939.
  10. Binelli M., Rocha C.C., Bennett A., Waheed A., Sultana H., Maldonado M.B.C. & Mesquita F.S. (2024) Solutions to the fertility equation in beef embryo recipients. Animal Reproduction 21, e20240041.
  11. Bishop J.V., Guzeloglu A., Scheller T., Docheff J.J., Gonzalez-Berrios C.L., Van Campen H., Nett T.M., Zezeski A.L., Geary T.W. & Thatcher W.W. (2025) Early identification of bovine pregnancy status and embryonic mortality. Biology of Reproduction 112, 981-95.
  12. Brenner M.A., Marques R.S., Posbergh C.J., Zezeski A.L., Geary T.W. & McCoski S.R. (2025) Maternal Injectable Mineral Administration Effects on Calf Growth and Reproductive Parameters. Animals 15, 330.
  13. Britz S.M., Nelson S., Earhart K.M., Pru J.K. & Schmitt E.E. (2024) Circadian Disruption Impacts Fetal Development in Mice Using High-Frequency Ultrasound. Journal of Circadian Rhythms 22, 4.
  14. Cain J.W., Seo H., Bumgardner K., Lefevre C., Burghardt R.C., Bazer F.W. & Johnson G.A. (2024) Pig conceptuses utilize extracellular vesicles for interferon-gamma-mediated paracrine communication with the endometrium. Biology of Reproduction 111, 174-85.
  15. Camacho L.E., Davis M.A., Kelly A.C., Steffens N.R., Anderson M.J. & Limesand S.W. (2022) Prenatal oxygen and glucose therapy normalizes insulin secretion and action in growth-restricted fetal sheep. Endocrinology 163, bqac053.
  16. Candelaria J.I., Botigelli R.C., Guiltinan C., Shikanov A. & Denicol A.C. (2025) Three-dimensional culture in a bioengineered matrix and somatic cell complementation to improve growth and survival of bovine preantral follicles. Journal of Assisted Reproduction and Genetics, 1-15.
  17. Candelaria J.I. & Denicol A.C. (2024) Assessment of ovarian tissue and follicular integrity after cryopreservation via slow freezing or vitrification followed by in vitro culture. F&S Science 5, 154-62.
  18. Carter R.E., Emenheiser J.C., Zinn S.A., Govoni K.E., Felix T.L. & Reed S.A. (2025) Effects of Milk Replacer Composition on Growth and Development of Beef x Dairy Crossbred Calves. Translational Animal Science, txaf005.
  19. Castillo-Salas C.A., Luna-Nevárez G., Reyna-Granados J.R., Luna-Ramirez R.I., Limesand S.W. & Luna-Nevárez P. (2023) Molecular markers for thermo-tolerance are associated with reproductive and physiological traits in Pelibuey ewes raised in a semiarid environment. Journal of Thermal Biology 112, 103475.
  20. Cavalcante de Souza D., Gonella-Diaza A.M., de Carvalho N.A.T., Elliff F.M., de Carvalho J.G.S., Vieira L.M., Bonfim-Neto A.P., de Carvalho Papa P., Ghuman S.S. & Madureira E.H. (2024) Supplementation with long-acting injectable progesterone 3 days after TAI impaired luteal function in buffaloes. Tropical Animal Health and Production 56, 76.
  21. Contreras-Correa Z.E., Sánchez-Rodríguez H.L., Arick II M.A., Muñiz-Colón G. & Lemley C.O. (2024) Thermotolerance capabilities, blood metabolomics, and mammary gland hemodynamics and transcriptomic profiles of slick-haired Holstein cattle during mid lactation in Puerto Rico. Journal of Dairy Science 107, 4017-32.
  22. Cuellar C.J., Amaral T.F., Rodriguez‐Villamil P., Ongaratto F., Martinez D.O., Labrecque R., Losano J.D.d.A., Estrada‐Cortés E., Bostrom J.R. & Martins K. (2024) Consequences of gene editing of PRLR on thermotolerance, growth, and male reproduction in cattle. FASEB BioAdvances 6, 223-34.
  23. Davenport K.M., Lockhart K., Stoecklein K., Schnabel R.D., Spencer T.E. & Ortega M.S. (2025a) Genome-wide association analyses identify single-nucleotide polymorphisms associated with in vitro embryo cleavage and blastocyst rates in Holstein bulls. Journal of Dairy Science.
  24. Davenport K.M., Lowke M.T., Ortega M.S., Kelleher A.M., Warren W.C. & Spencer T.E. (2025b) Single cell multiome analysis of the bovine placenta identifies gene regulatory networks in trophoblast differentiation. Biology of Reproduction 112, 955-68.
  25. Davenport K.M., O’Neil E.V., Ortega M.S., Patterson A., Kelleher A.M., Warren W.C. & Spencer T.E. (2024) Single-cell insights into development of the bovine placenta. Biology of Reproduction 110, 169-84.
  26. de Castro T., van Heule M., Domingues R.R., Jacob J.C., Daels P.F., Meyers S.A., Conley A.J. & Dini P. (2024) Embryo-endometrial interaction associated with the location of the embryo during the mobility phase in mares. Scientific Reports 14, 3151.
  27. Dias N.W., Poole R., Soffa D.R. & Brown K.J.H. (2024) Dynamic principles of the microbiome and the bovine vagina: a review. Frontiers in Microbiology 15, 1434498.
  28. Dobbins T.W., Swanson R.M., Dennis A.A., Rivera J.D., Dinh T.T., Lemley C.O. & Burnett D.D. (2024) Melatonin supplementation to sows in mid to late gestation affects offspring circadian, myogenic, and growth factor transcript abundance in pre and postnatal skeletal muscle. Journal of Animal Science 102, skae377.
  29. Domingues R.R., Wiltbank M.C., Hernandez L.L. & Adcock S.J. (2025) Prenatal treatment with the antidepressant fluoxetine on maternal and neonatal behavior in sheep. Pediatric Research, 1-6.
  30. Engle T.E., Guimaraes O., Loh H.Y., Thorndyke M.P., Van Campen H., Kincade J.N., Eder J.M. & Hansen T.R. (2024) Late gestation maternal infection with bovine viral diarrhea virus impacts offspring feedlot performance, digestion, blood parameters, and hot carcass weights. Journal of Animal Science 102, skae334.
  31. Fang L., Teng J., Lin Q., Bai Z., Liu S., Guan D., Li B., Gao Y., Hou Y. & Gong M. (2025) The Farm Animal Genotype–Tissue Expression (FarmGTEx) Project. Nature genetics, 1-11.
  32. Feltrin I.R., da Silva A.G., Rocha C.C., Ferraz P.A., da Silva Rosa P.M., Martins T., da Silveira J.C., Oliveira M.L., Binelli M. & Pugliesi G. (2024) Effects of 17β-estradiol on the uterine luteolytic cascade in bovine females at the end of diestrus. Theriogenology 213, 1-10.
  33. Fritsche K.L., Ahola J.K., Pinedo P.J., Seidel G.E., Rhoades R.D., Stevenson J.S., Olson K., Jaeger J.R., Grieger D.M. & Bromfield J.J. (2024) Pregnancy risk in beef and dairy cows after supplementing semen with transforming growth factor beta-1 at the time of artificial insemination. Journal of Animal Science 102, skae169.
  34. Geisert R.D., Bazer F.W., Lucas C.G., Pfeiffer C.A., Meyer A.E., Sullivan R., Johns D.N., Sponchiado M. & Prather R.S. (2024) Maternal recognition of pregnancy in the pig: a servomechanism involving sex steroids, cytokines and prostaglandins. Animal Reproduction Science, 107452.
  35. Gonella-Diaza A., Sponchiado M., Rodrigues França M., Liu L., Pugliesi G., Guimarães Lo Turco E., Peñagaricano F. & Binelli M. (2024) The metabolomic composition of the oviductal fluid is controlled by the periovulatory hormonal context in Bos indicus cows. Biology of Reproduction 111, 1188-201.
  36. Gonzalez Berrios C.L., Bowden C.F., Saad H.M., Bishop J.V., Van Campen H., Pinedo P., Hansen T.R. & Thomas M.G. (2024) Identification of candidate SNPs associated with embryo mortality and fertility traits in lactating Holstein cows. Frontiers in Genetics 15, 1409335.
  37. Goyal D., Limesand S.W. & Goyal R. (2023) Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis. International Journal of Molecular Sciences 24, 7483.
  38. Green K.L., Kovarna M., Menezes A.C.B., Schlegel E., Wright C., Smith Z.K. & Drum J. (2024) PSIII-10 Flaxseed oil-based supplement alters water consumption and plasma fatty acid profile of beef heifers. Journal of Animal Science 102, 503-4.
  39. Guiltinan C., Botigelli R.C., Candelaria J.I., Smith J.M., Arcanjo R.B. & Denicol A.C. (2025) Primed bovine embryonic stem cell lines can be derived at diverse stages of blastocyst development with similar efficiency and molecular characteristics. Biology Open 14, BIO061819.
  40. Guzeloglu A., Bishop J.V., Van Campen H., Plewes M.R., Gonzalez-Berrios C.L., Kincade J.N., Davis J.S. & Hansen T.R. (2024) Interferon-tau infusion into the ovine corpus luteum delays luteolysis. Biology of Reproduction 111, 667-77.
  41. Haimon M.L., Estrada-Cortés E., Amaral T.F., Block J., Jeensuk S., Maia T.S., Hoorn Q.A., Sagheer M., Bittar J.H. & Hansen P.J. (2024a) A low concentration of choline chloride alters the developmental program of the bovine preimplantation embryo. Reproduction and Fertility 5.
  42. Haimon M.L., Estrada-Cortés E., Amaral T.F., Martin H., Jeensuk S., Block J., Heredia D., Venturini M., Rojas C.S. & Gonella-Diaza A.M. (2024b) Provision of choline chloride to the bovine preimplantation embryo alters postnatal body size and DNA methylation. Biology of Reproduction 111, 567-79.
  43. He W., Posey E., Steele C., Savell J., Bazer F. & Wu G. (2024) Dietary glycine supplementation activates mTOR signaling pathway in tissues of pigs with intrauterine growth restriction. Journal of Animal Science, skae141-skae.
  44. Hess M.K., Mersha A., Ference S.S., Nafziger S.R., Keane J.A., Fuller A.M., Kurz S.G., Sutton C.M., Spangler M.L. & Petersen J.L. (2024) Puberty classifications in beef heifers are moderately to highly heritable and associated with candidate genes related to cyclicity and timing of puberty. Frontiers in Genetics 15, 1405456.
  45. Hong T., Park S., An G., Bazer F.W., Song G. & Lim W. (2024) Norflurazon causes cell death and inhibits implantation-related genes in porcine trophectoderm and uterine luminal epithelial cells. Food and Chemical Toxicology 186, 114559.
  46. Hou Y., Li X., Dai Z., Wu Z., Bazer F.W. & Wu G. (2018) Analysis of glutathione in biological samples by HPLC involving pre-column derivatization with o-phthalaldehyde. Polyamines: Methods and Protocols, 105-15.
  47. Jauregui E.J., McSwain M., Liu X., Miller K., Burns K. & Craig Z.R. (2023) Human-relevant exposure to di-n-butyl phthalate tampers with the ovarian insulin-like growth factor 1 system and disrupts folliculogenesis in young adult mice. Toxicological Sciences 195, 42-52.
  48. Johnson G.A., Bazer F.W., Burghardt R.C., Seo H., Wu G., Cain J.W. & Pohler K.G. (2024) The history of interferon-stimulated genes in pregnant cattle, sheep, and pigs. Reproduction 168.
  49. Kanzawa S., O’Meara E., Funnell B., Braz C., Bangert E., Cardoso F. & Wheeler M. (2024) 178 The effects of heat stress on the number of ovarian follicles in the dairy cow. Reproduction, Fertility and Development 37.
  50. Kelp N.C., Pru C.A., Paudel S., Lydon J.P., Kim J.J., Peluso J.J. & Pru J.K. (2025) Uterine Pgrmc2 Deficiency Attenuates Endometrial Hyperplasia and Cancer and Prolongs Lifespan in a Pten Loss-of-Function-Induced Cancer Model. Cancers 17, 1178.
  51. Kelson V., Kiser J., Davenport K., Suarez E., Murdoch B. & Neibergs H. (2025) Genomic regions associated with Holstein heifer times bred to artificial insemination and embryo transfer services. Genomics 117, 110972.
  52. Kelson V.C., Kiser J.N., Davenport K.M., Suarez E.M., Murdoch B.M. & Neibergs H.L. (2024) Identifying regions of the genome associated with conception rate to the first service in holstein heifers bred by artificial insemination and as embryo transfer recipients. Genes 15, 765.
  53. Kern C. & Liu W.-S. (2025) PRAMEY enhances sperm-egg binding and modulates epigenetic dynamics in bovine embryogenesis. Cell and Tissue Research, 1-12.
  54. Kincade J.N., Murtazina D.A., Georges H.M., Gonzalez-Berrios C.L., Bishop J.V., Engle T.E., Henao-Tamayo M., Eder J.M., McDonald E.M. & Deines D.M. (2025) Postnatal Epigenetic Alterations in Calves Persistently Infected with Bovine Viral Diarrhea Virus. Viruses 17, 708.
  55. Lee H., Lim W., Kweon J., Park J., Kim J., Bazer F.W., Song G. & Ham J. (2024) Resmethrin induces implantation failure by disrupting calcium homeostasis and forcing mitochondrial defects in porcine trophectoderm and uterine luminal epithelial cells. Science of The Total Environment 954, 176441.
  56. Lefevre C.M., Cain J.W., Kramer A.C., Seo H., Lopez A.N., Sah N., Wu G., Bazer F.W. & Johnson G.A. (2024) Evidence for metabolism of creatine by the conceptus, placenta, and uterus for production of adenosine triphosphate during conceptus development in pigs. Biology of Reproduction 111, 694-707.
  57. Lopez A.N., Bazer F.W. & Wu G. (2024) Functions and metabolism of amino acids in bones and joints of cats and dogs. In: Nutrition and Metabolism of Dogs and Cats (pp. 155-75. Springer.
  58. Lopez A.N., Newton M.G., Stenhouse C., Connolly E., Hissen K.L., Horner S., Wu G., Foxworth W. & Bazer F.W. (2025a) Dietary citrulline supplementation enhances milk production in lactating dairy goats. Journal of Animal Science and Biotechnology 16, 51.
  59. Lopez A.N., Olivarez M.A., Stenhouse C., Moses R.M., Newton M.G., Sah N., Seo H., Cain J., Lefevre C. & Ross A. (2025b) Effects of dietary supplementation of creatine on fetal development in gilts at d 60 and d 90 of gestation. Journal of Animal Science and Biotechnology 16, 31.
  60. Martins T., Rocha C.C., Driver J.D., Rae O., Elzo M.A., Mateescu R.G., Santos J.E.P. & Binelli M. (2024) Influence of proportion of Brahman genetics on productivity of Brahman–Angus cows at weaning. Translational Animal Science 8, txae093.
  61. McGlade E.A., Mao J., Stephens K.K., Marquardt R.M., Arguc F.N., Lais P.F., Wu S.-P., Winuthayanon S., Shirwan H. & Yolcu E.S. (2025) Progesterone signaling in oviductal epithelial cells modulates the immune response to support preimplantation embryonic development. Science Advances 11, eadt6113.
  62. Mendina G.R., de Brun V., de Lourdes Adrien M., Pons V., Paradizo R.V., Gil J., Rocha C.C., Binelli M. & Meikle A. (2025) Increased expression of interferon-stimulated gene 15 (ISG15) in cervical cells on day 14 of pregnancy in Holstein heifers. JDS Communications 6, 165-70.
  63. Monaco C.F., Jones C.M., Sayles H.R., Rudloff B., McFee R., Cupp A.S. & Davis J.S. (2025) Luteal fibroblasts produce prostaglandins in response to IL1β in a MAPK-mediated manner. Molecular and Cellular Endocrinology 596, 112420.
  64. Monteiro P.L., Wiltbank M.C., Frizzarini W.S., Andrade J.P.N., Cabrera E.M., Schoenfeld S.G., Domingues R.R. & Hernandez L.L. (2024) Hormonal profiles and biomarkers leading to parturition in cattle. Biology of Reproduction 111, 1282-96.
  65. Moses R.M., Stenhouse C., Halloran K.M., Sah N., Hoskins E.C., Washburn S.E., Johnson G.A., Wu G. & Bazer F.W. (2024) Metabolic pathways for glucose and fructose: I synthesis and metabolism of fructose by ovine conceptuses. Biology of Reproduction 111, 148-58.
  66. Nava-Trujillo H., Donnelly C.G. & Rivera R.M. (2025) Abnormal Offspring Syndrome. Assisted Reproductive Technologies in Animals Volume 2, 267-98.
  67. Newton M.G., Lopez A.N., Stenhouse C., Hissen K.L., Connolly E.D., Li X., Zhou L., Wu G., Foxworth W.B. & Bazer F.W. (2025) Impact of dietary supplementation of L-citrulline to meat goats during gestation on reproductive performance. Journal of Animal Science and Biotechnology 16, 5.
  68. on Aging F., Nass S., Berkower C., Cooper R., National Academies of Sciences E. & Medicine (2022) Proceedings of a Workshop. In: Companion Animals as Sentinels for Predicting Environmental Exposure Effects on Aging and Cancer Susceptibility in Humans: Proceedings of a Workshop. National Academies Press (US).
  69. Palcheff L.J., VanWye G.M., Ricardo K.R., Green K.L., Even F.J., Roberts S.R., Lonas A.B., Spinka C.M., Poock S.E. & Menegatti Zoca S. (2025) Tandem Administration of Prostaglandin F2α and Gonadotropin-Releasing Hormone in Beef Heifers and Cows as a Convergent Presynchronization Method in the 7 & 7 Synch Protocol. Animals 15, 1329.
  70. Park J., An G., Lee H., Park S., Ham J., Bazer F.W., Song G. & Lim W. (2024a) Beta-cyfluthrin impairs implantation process by inducing mitochondrial defects and changes in reactive oxygen species-mediated signaling pathways in porcine trophectoderm and uterine luminal epithelial cells. Science of The Total Environment 934, 173097.
  71. Park J., Lee H., Kweon J., Park S., Ham J., Bazer F.W. & Song G. (2024b) Mechanisms of female reproductive toxicity in pigs induced by exposure to environmental pollutants. Molecules and cells, 100065.
  72. Pendleton A.L., Limesand S.W. & Goyal R. (2023) In Vivo Real-Time Study of Drug Effects on Carotid Blood Flow in the Ovine Fetus. Journal of Visualized Experiments (JoVE), e64551.
  73. Plewes M.R., Talbott H.A., Schott M.B., Wood J.R., Cupp A.S. & Davis J.S. (2024) Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells. The FASEB Journal 38, e23710.
  74. Poole R.K., Soffa D.R., McAnally B.E., Smith M.S., Hickman-Brown K.J. & Stockland E.L. (2023) Reproductive microbiomes in domestic livestock: insights utilizing 16S rRNA gene amplicon community sequencing. Animals 13, 485.
  75. Posey E.A., He W., Steele C.C., Savell J.W., Bazer F.W. & Wu G. (2024) Dietary glycine supplementation enhances creatine availability in tissues of pigs with intrauterine growth restriction. Journal of Animal Science 102, skae344.
  76. Posont R.J., Most M.S., Cadaret C.N., Marks-Nelson E.S., Beede K.A., Limesand S.W., Schmidt T.B., Petersen J.L. & Yates D.T. (2022) Primary myoblasts from intrauterine growth-restricted fetal sheep exhibit intrinsic dysfunction of proliferation and differentiation that coincides with enrichment of inflammatory cytokine signaling pathways. Journal of Animal Science 100, skac145.
  77. Przygrodzka E., Bhinderwala F., Powers R., McFee R.M., Cupp A.S., Wood J.R. & Davis J.S. (2025) Metabolic control of luteinizing hormone-responsive ovarian steroidogenesis. Journal of Biological Chemistry 301.
  78. Reis T.R.L., Lopes-Ferreira J.V., Guerra K.B., da Silva L.A.B., Stenhouse C., Ashworth C.J., Bloise E., Chiarini-Garcia H. & de Almeida F.R.C.L. (2025) The structure of the porcine uterine-conceptus interface is associated with gestational day, fetal size and sex. Domestic Animal Endocrinology 90, 106895.
  79. Rocha C.C., Cordeiro A.L.L., Campbell M., Maldonado M.B.C., Silva F.A.C.C., Bennett A., Waheed A., Hansen T. & Binelli M. (2024) In vitro reminiscence: uterine programming in vivo affects respective luminal epithelial cells function in vitro. Biology of Reproduction 111, 600-12.
  80. Rocha C.C., Montevecchio A.B., Bennett A., Waheed A., Mazziotta M., Maia T.S., Haimon M.L.-J., Hoorn Q.A., Sagheer M. & Cuellar C.J. (2025) Relationships between activity monitoring device data and ovarian, uterine, hormonal, and pregnancy variables in beef cows. Theriogenology 235, 64-74.
  81. Sah N., Stenhouse C., Halloran K.M., Moses R.M., Newton M.G., Seo H., Cain J.W., Lefevre C.M., Johnson G.A. & Wu G. (2025) Effect of gestational age and fetal sex on metabolism of creatine by uteri, placentae, and fetuses of pigs. Biology of Reproduction, ioaf015.
  82. Seo H., Bazer F.W. & Johnson G.A. (2023) Early syncytialization of the ovine placenta revisited. Syncytia: Origin, Structure, and Functions, 127-42.
  83. Sitko E., Laplacette A., Duhatschek D., Rial C., Perez M., Tompkins S., Kerwin A., Domingues R., Wiltbank M.C. & Giordano J. (2024) Ovarian function and endocrine phenotypes of lactating dairy cows during the estrous cycle are associated with genomic-enhanced predictions of fertility potential. Journal of Dairy Science 107, 7352-70.
  84. Skjold V., Afanasyev S., Burgerhout E., Sveen L., Rørvik K.-A., Mota V.F.C.N., Dessen J.-E. & Krasnov A. (2024) Endocrine and Transcriptome Changes Associated with Testicular Growth and Differentiation in Atlantic Salmon (Salmo salar L.). Current Issues in Molecular Biology 46, 5337-51.
  85. Smith M.S., Soffa D.R., McAnally B.E., Hickman-Brown K.J., Stockland E.L. & Poole R.K. (2024) On-farm study: cytokine profiles and vaginal microbiome of Bos indicus cattle before artificial insemination. Frontiers in Animal Science 5, 1399337.
  86. So W., Dong R., Lee D., Abazarikia A., Hackfort B.T., Cupp A.S. & Kim S.-Y. (2025) Primordial follicle survival and changes in ovarian vasculature may be independently regulated during chemotherapy. Endocrinology 166, bqaf059.
  87. Stella S.L., Guadagnin A.R., Velasco-Acosta D.A., Ferreira C.R., Rubessa M., Wheeler M.B., Luchini D. & Cardoso F.C. (2024) Rumen-protected methionine supplementation alters lipid profile of preimplantation embryo and endometrial tissue of Holstein cows. Frontiers in Veterinary Science 10, 1301986.
  88. Stenhouse C., Donadeu F.X. & Almeida F.R. (2024a) Intrauterine growth restriction: screening and outcomes. Frontiers in Physiology 15, 1438381.
  89. Stenhouse C., Halloran K.M., Hoskins E.C., Moses R.M., Newton M.G., Sah N., Wolpo Y.E., Tyree M.F., Seo H. & Johnson G.A. (2025) Characterization of the expression of XPR1 in ovine utero-placental tissues. Reproduction 169.
  90. Stenhouse C., Halloran K.M., Hoskins E.C., Moses R.M., Wu G., Seo H., Johnson G.A., Suva L.J., Gaddy D. & Bazer F.W. (2024b) Progesterone regulates tissue non-specific alkaline phosphatase (TNSALP) expression and activity in ovine utero-placental tissues. Journal of Animal Science and Biotechnology 15, 90.
  91. Stockland E.L., Smith M.S., Pickett A.T., Cooke R.F. & Poole R.K. (2024) Influence of differing levels of concentrate on circulating cytokine concentrations in beef heifers. Translational Animal Science 8, txae089.
  92. Suarez E.M., Kelson V.C., Kiser J.N., Davenport K.M., Murdoch B.M., Herrick A.L. & Neibergs H.L. (2025) Loci associated with spontaneous abortion in primiparous Holstein cattle. Frontiers in Veterinary Science 12, 1599401.
  93. Suarez E.M., Kelson V.C., Kiser J.N., Davenport K.M., Murdoch B.M. & Neibergs H.L. (2024) Genomic Regions Associated with Spontaneous Abortion in Holstein Heifers. Genes 15, 1498.
  94. Swanson R.M., Messman R.D., Dobbins T.W., Contreras-Correa Z.E., Arick M.A., Sidelinger D.R., King H. & Lemley C.O. (2024) Seminal plasma uterine priming alters uterine transcriptomics and negatively impacts embryo growth and uterine artery resistance but not offspring liver transcriptomics in beef cattle. Journal of Animal Science 102, skae300.
  95. Tillquist N.M., Govoni K.E., Zinn S.A. & Reed S.A. (2025) Poor maternal nutrition during gestation in sheep alters key hormonal systems involved in energy homeostasis and appetite in the offspring. Domestic Animal Endocrinology 91, 106907.
  96. Tyree M.F. & Stenhouse C. (2025) Exogenous progesterone supplementation: a strategy to enhance conceptus development in sheep and pigs? Reproduction and Fertility 6.
  97. Ungerfeld R. & Alexander B.M. (2024) Determinants of ram sexual behavior and its impact on sheep breeding. Animal Reproduction Science, 107599.
  98. Van Campen H., Bishop J.V., Brink Z., Engle T.E., Gonzalez-Berrios C.L., Georges H.M., Kincade J.N., Murtazina D.A. & Hansen T.R. (2024) Epigenetic modifications of white blood cell DNA caused by transient fetal infection with bovine viral diarrhea virus. Viruses 16, 721.
  99. Wu G., Bazer F.W., Johnson G.A., Satterfield M.C. & Washburn S.E. (2024) Metabolism and nutrition of L-glutamate and L-glutamine in ruminants. Animals 14, 1788.
  100. Yang M., Diaz F., Krause A.R.T., Lei Y. & Liu W.-S. (2024) Synergistic enhancement of the mouse Pramex1 and Pramel1 in repressing retinoic acid (RA) signaling during gametogenesis. Cell & Bioscience 14, 28.
Back
Log Out ?

Are you sure you want to log out?

Press No if you want to continue work. Press Yes to logout current user.

Yes
Report a Bug
Report a Bug

Describe your bug clearly, including the steps you used to create it.