SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Jose I Reyes-De-Corcuera (Florida), Jeong-Yeol Yoon (Arizona), Chenxu Yu (Iowa), Olga Tsyusko and Jarad Cochran (Kentucky), Ranee Anderson and Sam Nugen (Cornell), Evangelyn Alocilja (Michigan), Mengshi Lin (Missouri), Yi-Cheng Wang (Illinois), Sundaram Gunasekaran (Wisconsin), Anhong Zhou (Utah)

Station reports were delivered from each participant describing completed and on-going research.

Administrative updates by Hongda Chen and Amy Gruden (on behalf of Steve Lommel). Hongda Chen gave an overview of NIFA investments in nanotechnology R&D and education from 2003 through 2024, and fiscal 2025 plans and priorities including Nanotechnology for Agricultural and Food (Priority A1511) and Social Implications of Food and Agricultural Technologies (Priority A1642). He also highlighted key NIFA accomplishments for National Nanotechnology Initiative (NNI) goals, particularly Goal#4, 2, 3. He covered the Gordon Research Conference (GRC) – Nanoscale Science and Engineering for Agriculture and Food Systems on June 22-28, 2024, which was chaired by NC1194 member Dr. Carmen Gomes from Iowa State University. Finally, he gave some thoughts for NC-1194 discussion during the meeting, including recruiting initiative to bring more potential new members, exploring attending other multistate projects (e.g., S1077 Enhancing Microbial Food Safety by Risk Analysis) to discuss potential collaborations, approaches for commercialization of nano sensors, and enhancing research on micro- and nanoplastics. He suggested future meeting in conjunction with other professional meetings, such GRC IBE, and so on.

Amy Gruden reminded that this NC-1194 project expires September 2026, and it typically takes one year to start the preparation of renewal submission. Amy passed Steve Lommel’s message to remind the election of new officers. Steve encouraged NC-1194 group to resubmit the application of the North Central Regional Association’s (NCRA) Multistate Research award in the coming year.  Steve also encouraged the NC-1194 to work with other multi-state group, especially food safety group.

The meeting was organized by current Chair Chenxu Yu and completed the election of officers for the 2024-2025 starting immediately after the meeting: Anhong Zhou as the Chair, Zhengrong Gu as the Vice-Chair, Yi-Cheng Wang as the Secretary. The remainder of the meeting was devoted to discussing options for 2025 meeting (tentatively August 11-12 in Salt Lake City, UT), collaborations, joint publications, grant applications, and exploring collaboration with another multistate project S1077 “enhancing microbial food safety by risk analysis”. and rewrite a conference proposal co-sponsored by NC1194 and S1077 for NIFA. Full minutes are available at NIMSS website under Reports.

Lommel, Steve (slommel@ncsu.edu) – North Carolina State University / project administrator; Chen, Hongda (hongda.chen@usda.gov) – USDA-NIFA.

Accomplishments

Contributions to the objectives of this project on its first year are summarized. At least 49 publications last year associated to the research and listed and the end of this report were published by the NC-1194 group.

  1. Develop new technologies for characterizing fundamental nanoscale processes and fabricate self-assembled nanostructures

Outputs: Spectroscopic imaging methods for analyzing protein-nanoplastics interactions were developed, and methods to better understand the soil amendment mechanisms to reduce continuous cropping obstacle for potato were applied (IA). SERS-based nanosensor was developed by molecular self-assembly approaches to detect four pesticides, thiabendazole, thiram, endosulfan, malathion, at the limits of detection (LOD) of 44-88 µg/kg strawberry extract (MO). Nanocarriers for drug/vaccine delivery and functional food with enhanced nutritional values and quality were developed (IA). Novel nanoparticles-based adjuvants for vaccine to treat porcine epidemic diarrhea virus was developed (VT). Electrospun nanofiber-based electrochemical biosensor was developed to detect multiple agriculture related targets (WI). A novel electrochemical biosensor platforms using graphene coated magnetic nanoparticles or microfluidic chips were designed and fabricated for detection of infectious pathogens such as wine influenza A and penicillin-resistant Salmonella with rapid response (< 30 min), high sensitivity (10-19 mol/L, equal to 100 pathogen/ml) and fast detection (< 30 min) of the target DNA or RNA of pathogens (SD).

  1. Develop devices and systems incorporating nanotechnology and data-driven analytics for detection of biological/chemical targets, with an emphasis on detection of infectious diseases in plants, animals, humans, and the environment

Outputs: Research on the characterization and understanding of environmental health toxicity and toxicity mechanisms of the various nanomaterials used in agriculture before and after environmental transformations. Transformation, interaction, and toxicity of emerging 2D nanomaterials free-standing and embedded onto nanocomposite membranes for PFAS degradation were explored. Zn- and Fe-dopped hydroxyapatite nanoparticles for nitrogen delivery was developed in a safety-by-design approached (KY). Methods to better understand and improve the mechanisms of spreading of antibiotic-resistant genes during manure composting were developed (IA). Portable gene-based and immunosensors for rapid and label-free detection of SARS-CoV-2 in saliva were developed (IA). First generation phosphate sensors based on stimulus-response nanobrush electrodes or on carbon nanoparticles, nitrogen sensors based on laser inscribed graphene electrodes, and Listeria spp. as well as Salmonella sensors were developed (IA). Sensor-based technologies were developed to monitor antibiotic-resistant bacteria during fresh produce production using Municipal Wastewater Effluents (IA). The concentration of nano/microplastics was quantified in 132 Iowa lakes, identified the impact of the presence of bioturbators (e.g. midge fly larvae) on the behavior and distribution of microplastics in aquatic ecosystems, and assessed the impact of nanoplastics on the development of Drosophila (IA). The team in MSU developed and validated the SMART biosensor to rapidly extract and detect foodborne pathogens, such as Salmonella enterica, E. coli O157:H7, Staphylococcus aureus, and Bacillus cereus from large-volume complex food and farm samples, such as turkey and chicken meats, rinsates from turkey and chicken processing plants, poultry manure, and cattle manure. They also developed the African Swine Fever Biosensor to detect ASF in farm settings rapidly. The ASF biosensor is being validated in ASF-infected farm samples by our international collaborators (MI). The Utah State team investigated cellular oxidative stress in response to polystyrene (PS) nanoparticles (PS50 for 50 nm, PS500 for 500 nm, and PS100-NH2 for 100 nm with amino group (UT). A novel concept of using a set of biomolecules and subsequent ML classification has been proposed and successfully demonstrated for detecting environmental toxicants, including microplastics, nanoplastics, and PFAS, from various environmental samples (aerosols, particulate matter, soil, and wastewater). This method was also demonstrated for identifying the lung disease biomarker (eNAMPT) from human blood plasma samples. A smartphone-based multi-spectral fluorescence imaging system was designed, fabricated, and demonstrated for detecting microalgae from seawater and microbiota from field soil samples (AZ). A CRISPR-based biosensor was developed to detection of antimicrobial-resistant genes in Carbapenem-resistant Enterobacterales (IL). The engineering of bacteriophages was developed for isolation, concentration and detection of Salmonella in agriculture water (NY).

  1. Advance the integration of novel sensor networks, information systems, and artificial intelligence for effective risk assessment and decision support for food security and safety

Outputs: A new textbook on machine learning (ML) and artificial intelligence (AI) in chemical and biological sensing published by Elsevier in 2024 was jointly edited by NC1194 members Chenxu Yu (IA) and Jeong-Yeol Yoon (AZ). A smartphone-based multi-spectral fluorescence imaging technology was developed for detection of microalgae and soil microbiota (AZ). ML-based SERSFormer technology was recently developed to highly sensitively and specifically detect pesticides in various agricultural samples (MO). Machine learning (ML) tools were created to analyze Raman spectra to determine the cellular oxidative stresses induced by exposure of nanoplastics (UT). An intelligent packaging technology for food quality monitoring was developed (IL).

  1. Develop and update education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment.

Outputs: The Michigan state team continued hosting the 2024 GARD Forum virtually on March 14-16, 2024, with >700 registered participants from 39 countries in six continents. Technical sessions, short courses, and an Innovation Challenge were held during the forum with involvement of 35 teams that led to 6 finalists. The team also demonstrated the technology developed by them at the MSU Science Festival in April 2024. Short courses on Nanotechnology were taught (KY), course on biosensors was developed and taught (FL). A REU-site on wearable graphene-based stress biosensor development with community college and high school students starting summer 2021 continued through 2023-2024 (IA). A USDA conference grant “One Health” was awarded to the team (IA) to support the 2024 Annual Institute of Biological Engineering (IBE) conference in Atlanta, GA on September 14-15, 2024.

  1. Increase the number academic-industry partnerships to help move the developed technologies to commercialization phase.

IA station continued working with an NSF-funded IUCRC team to improve academic-industry partnership and develop technologies for soil dynamics to promote commercialization of the technology (IA). The biosensors developed by MSU team (MI) has initiated the connections with the local turkey and chicken industry.

Impacts

  1. The research carried out by the group increased the understanding of nanotechnology and biosensors by the general public by bringing greater awareness of their current and potential roles in food and agriculture. At least 6 postdocs, 28 PhD students, 9 MS students, 25 undergraduate students, and 1 visiting scholar participated in this project. 1. Fabrication of high-performance substrates for SERS via electrospinning with self-assembly was achieved to successful detection of pesticides in foods with improved sensitivity and specificity (MO). 2. Several cross-country and cross-continent collaborations were formed through the GARD. The GARD-Asia-Africa Initiative (GARD-AAI) conducted workshops on nanotechnology and biosensors attended by scientists from both continents (MI). 3. We trained four female undergraduates and two minority students under the Summer Research Opportunity Program (SROP), a gateway to graduate education at Big Ten Academic Alliance universities, whose goal is to increase the number of underrepresented students to pursue graduate study and research careers. We also trained one minority student under the Engineering Summer Undergraduate Research Experience (EnSURE), an "internship in graduate school" program that provides participants an early opportunity to participate in research by working with faculty mentors. These students presented their work at the 2024 Mid-SURE event, where they learned the skills of public engagement and articulated the potential impact of their work on society’s real needs (MI). We demonstrated our Raman measurement of living cells exposed to nanoplastics to four native American college students (UT). 4. We also demonstrated our Salmonella biosensor to staff from the Michigan Turkey Producers and the Miller Poultry. These stakeholders received attention to nanotechnology-enabled biosensors that are capable of rapidly inform and control Salmonella contamination in turkey and chicken production systems (MI). 5. Study of adjuvants that can enhance the efficacy of nanovaccines would greatly reduce porcine epidemic diarrhea virus (PEDV) (VT). 6. Our research has increased our understanding of the effects of HHP on enzyme stability from the structural perspective (FL). This new knowledge will serve as the foundation for the stabilization of enzyme-based biosensors. 7. We designed, developed, and tested a smartphone-based multi-spectral fluorescence imaging system for detection of microalgae from seawater and microbiota from field soil samples (AZ) 8. We developed different biosensor technologies and methods for micro- and nanoplastics (including PFAS) detection and cells-MNPs interaction induced toxicity (IA, AZ, UT, KY) 9. We developed and validated novel electrochemical biosensor platforms that use graphene coated magnetic nanoparticles or microfluidic chips to detect DNA of specific microbes (SD) Outcomes Participants at MSU Received funding from the Michigan Alliance for Animal Agriculture on “Optimizing a nano-biosensor for rapid detection of the African swine fever and from the MSU AgBioResearch for a project on “Development of a Biosensing System for Rapid and Integrated Genome-to-Phenome Antimicrobial Resistance Testing (BRIGHT)”. AZ group received university’s One Health Initiative for a project entitled “Machine learning- and paper microfluidic-based classification of nanoplastics and identification of biologically interacting molecules from soil, plant, animal, and human samples”. Through an NSF funded IUCRC grant, the team from IA is collaborating with the researchers from Univ. of Florida, Univ. of Connecticut, Univ. of Washington, and Univ. of South California to develop soil dynamics technologies. UT team received funding from USDA-ARS Forage and Range Research Laboratory (FRRL) to conduct chemical composition analysis of wheat lines with salt tolerance. The SD team received funding from NASA-EPSCoR, NSF-EPSCOR, Sun Grant (North Central Center), South Dakota Oilseeds Initiative, South Dakota Oilseeds Council, South Dakota Beef Industrial Council, and USDA-NIFA.

Publications

  1. Yan Liang, Bradley Khanthaphixay, Jocelyn Reynolds, Preston J. Leigh, Melissa L. Lim, and Jeong-Yeol Yoon, "A Smartphone-Based Approach for Comprehensive Soil Microbiome Profiling," Applied Physics Reviews, 2024, 11(3): 031412.
  2. Jeong-Yeol Yoon and Chenxu Yu, Editors, "Machine Learning and Artificial Intelligence in Chemical and Biological Sensing," Elsevier: Amsterdam/London/Cambridge, 2024, ISBN: 978-0-443-22001-2 (paperback), 978-0-443-22000-5 (eBook).
  3. Jeong-Yeol Yoon, "Chapter 10 - ML-Assisted Biosensors Utilizing a Set of Biological Polymers," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.259-274.
  4. Chenxu Yu and Jeong-Yeol Yoon, "Chapter 4 - ML-Assisted E-Nose and Gas Sensors," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.83-112.
  5. Jeong-Yeol Yoon, "Chapter 3 - Use of ML/AI in Chemical Sensors and Biosensors," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.71-81.
  6. Yan Liang and Jeong-Yeol Yoon, "Chapter 2 - Fundamentals of Machine Learning," in Machine Learning and Artificial Intelligence in Chemical and Biological Sensing, Editors: Jeong-Yeol Yoon and Chenxu Yu, Elsevier: Amsterdam/London/Cambridge, 2024, pp.23-70.
  7. Yan Liang and Jeong-Yeol Yoon, "Sensors for Blood Brain Barrier on a Chip," Vitamins and Hormones, 2024, 126, 219-240.
  8. Bailey C. Buchanan, Yisha Tang, Hannah A. Lopez, Nancy G. Casanova, Joe G.N. Garcia, and Jeong-Yeol Yoon, "Development of a Cloud-Based Flow Rate Tool for eNAMPT Biomarker Detection," PNAS Nexus, 2024, 3(5): pgae173.
  9. Chloe Thomas, Togzhan Spatayeva, Dawon Yu, Andrew Loh, Un Hyuk Yim, and Jeong-Yeol Yoon, "A Comparison of Current Analytical Methods for Detecting Particulate Matter and Micro/Nanoplastics," Applied Physics Reviews, 2024, 11(1): 011313.
  10. Sinyang Kim, Katelyn Sosnowski, Dong Soo Hwang, and Jeong-Yeol Yoon, "Smartphone-Based Microalgae Monitoring Platform Using Machine Learning," ACS ES&T Engineering, 2024, 4(1): 186-195.
  11. Tyler Hertenstein, Yisha Tang, Alexander S. Day, Jocelyn Reynolds, Patrick V. Viboolmate, and Jeong-Yeol Yoon, "Rapid and Sensitive Detection of miRNA Via Light Scatter-Aided Emulsion-Based Isothermal Amplification Using a Custom Low-Cost Device," Biosensors and Bioelectronics, 2023, 237: 115444.
  12. Yang J, Yu, XL, Dong XP, Yu C, Improvement of surimi gel from frozen stored silver carp, Gels, 10(6), 374, 2024, https://doi.org/10.3390/gels10060374
  13. Xing Y, Zhang P, Zhang W, Yu C, Luo Z, Continuous cropping of potato changed the metabolic pathway of root exudates to drive rhizosphere microflora, Frontiers in Microbiology, 14, 1318586, 2024, https://doi.org/10.3389/fmicb.2023.1318586
  14. Tong T, Qi Y, Bussiere L, Dhar D, Miller C, Yu C, Wang Q, Rational Design of Oral Vaccines by Gut Organoid Mucosal Models, Bioactive Materials, 2023, 30, 116-128, https://doi.org/10.1016/j.bioactmat.2023.07.014
  15. Zhao M, Cao X, Wu Y, Zou S, Li Z, Lin X, Ji C, Dong L, Zhang S, Yu C, Liang H, Effects of prebiotics on the fermentation of traditional suancai of Northeast China, Food Science and Human Wellness, 2023, https://doi.org/10.26599/FSHW.2022.9250114
  16. Zhang W, Yu C, Yin S, Chang K, Chen K, Xing Y, Yang Y, Transmission and retention of antibiotic resistance genes (ARGs) in chicken and sheep manure composting, Bioresource Technology, 382, 129190, 2023,. https://doi.org/10.1016/j.biortech.2023.129190
  17. He Q, Habib F, Tong T, Yu C, Raman Spectroscopy for Detection of Foodborne Pathogens, Chemical Contaminants and Nanoparticles, in Encyclopedia of Food Safety, edited by Byron Brehm-Stecher, Elsevier, 2024.
  18. Roque JV, Pola CC, Terra LR, Oliveira TV, Teofilo RF, Gomes CL, Soares NFF, Mapping the Distribution of Additives Within Polymer Films Through Near-Infrared Spectroscopy and Hyperspectral Imaging, in Food Packaging Materials: Current Protocols, pp 183-203. 2024
  19. Qian H, Moreira G, Vanegas D, Tang Y, Pola C, Gomes C, McLamore E, Bliznyuk N, Improving high throughput manufacture of laser-inscribed graphene electrodes via hierarchical clustering, Scientific Reports, 1, 7980, 2024
  20. Beata M Szydlowska, Cícero C Pola, Zizhen Cai, Lindsay E Chaney, Janan Hui, Robert Sheets, Jeremiah Carpenter, Delphine Dean, Jonathan C Claussen, Carmen L Gomes, Mark C Hersam, Biolayer-Interferometry-Guided Functionalization of Screen-Printed Graphene for Label-Free Electrochemical Virus Detection, ACS Applied Materials & Interfaces, 16(19), 25169-25180, 2024
  21. Sara L Silvestre, Maria Morais, Raquel RA Soares, Zachary T Johnson, Eric Benson, Elisabeth Ainsley, Veronica Pham, Jonathan C Claussen, Carmen L Gomes, Rodrigo Martins, Elvira Fortunato, Luis Pereira, João Coelho, Green Fabrication of Stackable Laser‐Induced Graphene Micro‐Supercapacitors under Ambient Conditions: Toward the Design of Truly Sustainable Technological Platforms, Advanced Materials Technologies, 2400261, 2024
  22. J Claussen, J Hondred, Enhanced 3D porous architectured electroactive devices via impregnated porogens, US Patent 11,971,383
  23. Nathan M Jared, Zachary T Johnson, Cicero C Pola, Kristi K Bez, Krishangee Bez, Shelby L Hooe, Joyce C Breger, Emily A Smith, Igor L Medintz, Nathan M Neihart, Jonathan C Claussen, Biomimetic laser-induced graphene fern leaf and enzymatic biosensor for pesticide spray collection and monitoring, Nanoscale Horizons, 9(9), 1543-1556, 2024
  24. Jelena Stanković, Djuradj Milošević, Momir Paunović, Boris Jovanović, Nataša Popović, Jelena Tomović, Ana Atanacković, Katarina Radulović, Davor Lončarević, Maja Raković, Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates, Water, 16(7), 962, 2024
  25. Khouloud Sebteoui, Djuradj Milošević, Jelena Stanković, Viktor Baranov, Boris Jovanović, Stefan Krause, Zoltán Csabai, Beneath the surface: Decoding the impact of Chironomus riparius bioturbation on microplastic dispersion in sedimentary matrix, Science of the Total Environment, 919, 170844, 2024
  26. Alyssa M Hohman, Rachel Sorenson, Boris Jovanovic, Elizabeth McNeill, The heart of plastic: utilizing the Drosophila model to investigate the effects of micro/nanoplastics on heart function, Frontiers in Toxicology, 6, 1438061, 2024
  27. Rachel M Sorensen, Dimitrija Savić-Zdravković, Boris Jovanović, Changes in the wing shape and size in fruit flies exposed to micro and nanoplastics, Chemosphere, 363, 142821, 2024.
  28. Ghazy A, Nyarku R, Faraj R, Bentum K, Woube Y, Williams M, Alocilja E, and Abebe W. 2024. Gold Nanoparticle-based Plasmonic Detection of Escherichia coli, Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes from bovine fecal Samples, Microorganisms, 12, 1069.
  29. Caliskan-Aydogan1 O, Zaborney Kline C1, and Alocilja EC3. 2024. Cell Morphology as Biomarker of Carbapenem Exposure, Journal of Antibiotics, 77, pages 600–611 (2024).
  30. Caliskan-Aydogan1 O and Alocilja EC3. 2024. A Parallel biosensor platform for the detection of carbapenemase-producing coli in spiked food and water samples, Food Control, Vol. 163, 110485.
  31. Caliskan-Aydogan O1, Sharief S1, and Alocilja EC3. 2023. Rapid Isolation of Low-level Carbapenem-Resistant E. coli from Water and Foods Using Glycan-Coated Magnetic Nanoparticles, Biosensors 2023, 13(10), 902.
  32. Caliskan-Aydogan O1 and Alocilja EC3. 2023. A Review of Carbapenem Resistance in Enterobacterales and Its Detection Techniques. Microorganisms, 2023, 11(6), 1491.
  33. Boodoo C1, Dester E1, David J1, Patel V1, Rabin KC, and Alocilja EC3. 2023. Multi-Probe Nano-Genomic Biosensor to Detect aureus from Magnetically-Extracted Food Samples, Biosensors, 2023, 13(6), 608, https://doi.org/10.3390/bios13060608.
  34. Sharief S1, Caliskan-Aydogan O1, Alocilja EC3. 2023. Carbohydrate-coated nanoparticles for PCR-less genomic detection of Salmonella from fresh produce, Food Control, Vol. 150, 109770.
  35. Boodoo C1, Dester E1, Sharief S1, and Alocilja EC3. 2023. Influence of Biological and Environmental Factors in the Extraction and Concentration of Foodborne Pathogens using Glycan-Coated Magnetic Nanoparticles, Journal of Food Protection, 86(4):100066.
  36. Sharief SA1, Caliskan-Aydogan O1, and Alocilja EC3. 2023. Carbohydrate-coated nanoparticles for point-of-use food contamination testing, Biosensors and Bioelectronics: X, 13 (2023), 100322, 9 pp.
  37. Bhattarai RK1,3, Basnet HB, Dhakal IP, Alocilja E. 2023. Virulence genes of avian pathogenic Escherichia coli isolated from commercial chicken in Nepal, Comparative Immunology, Microbiology and Infectious Diseases, 95 (2023) 101961.
  38. Caliskan-Aydogan O1, Sharief S1, and Alocilja EC3. 2023. Nanoparticle-Based Plasmonic Biosensor for the Unamplified Genomic Detection of Carbapenem-Resistant Bacteria, Diagnostics, 2023, 13(4), 656.
  39. Hajikhani, M., Hegde, A., Snyder, J., Cheng, J., Lin, M. 2024. Integrating transformer-based machine learning with SERS technology for the analysis of hazardous pesticides in spinach. Hazard. Mater. 470, 134208.
  40. Hajikhani, M., Kousheh, S., Lin, M. 2024. Design of a novel SERS substrate by electrospinning for the detection of thiabendazole in soy-based foods. Food Chem. 436, 137703.
  41. Zhai, K., Sun, L., Nguyen, T., Lin, M. 2024. Facile synthesis of gold nanostars for duplex detection of pesticide residues in grapes using SERS. Food Sci. 89, 2512-2521.
  42. Alsammarraie, F. K., Lin, M., Mustapha, A. 2023. Green synthesis of silver nanomaterials and evaluation of their antibacterial and antioxidant effectiveness in chicken meat. Food Biosci. 56, 103332.
  43. Hajikhani, M., Zhang, Y., Gao, X., Lin, M. 2023. Advances in CRISPR-based SERS detection of food contaminants: A review. Trends Food Sci. Technol. 138, 615-627.
  44. Wang, W., Yu, Z., Lin, M., Mustapha, A. 2023. Toxicity of silver nanoparticle incorporated-bacterial nanocellulose to human cells and intestinal bacteria.  J. Biol. Macromol. 241, 124705.
  45. Weng, Z., You, Z., Li, H., Wu, G., Song, Y., Sun, H., Fradlin, A., Neal-Harris, C., Lin, M., Gao, X., Zhang, Y. 2023. CRISPR-Cas12a biosensor array for ultrasensitive detection of unamplified DNA with single-nucleotide polymorphic discrimination. ACS Sensors. 8(4), 1489-1499.
  46. M Liang, L Yuan, C Shao, X Zheng, Q Song, Z Gu, S Lu; 2023, Sequential Visual Sensing of H2O2 and GSH Based on Fluorescent Copper Nanoclusters Incorporated Eggshell Membrane, IEEE Sensors Journal (accepted)
  47. Liang, T. Schaffer, A. Sobhan, M. Biesecker, Z. Yang, C. Han, J. Hu, A. Smirnova, Z. Gu, 2023, 3D Cu Pyramid Array Grown on Planar Cu Foil for Stable and Dendrite–Free Lithium Deposition, Materials Science Vol.29 No.4, 2023 (accepted)
  48. Y Liang, W Ding, B Yao, F Zheng, A Smirnova, Z Gu, 2023, Mediating Lithium Plating/Stripping by Constructing 3D Au@ Cu Pentagonal Pyramid Array, Batteries 9 (5), 279
  49. Yuanzhi Bian,* Debra L. Walter,* Chenming Zhang. Efficiency of interferon-γ in activating dendritic cells and its potential synergy with toll-like receptor agonists. Viruses (MDPI). 2023, 15, 1198. doi.org/10.3390/v15051198.
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