Brief Summary of Minutes of Annual Meeting

W4112 Multistate Meeting 2023 Minutes

Fairbanks, Alaska

Monday, May 22, 2023

In attendance

Virtual: Ky Pohler-TAMU, Karl Kerns-Iowa State University, Caleb Lemley- Mississippi State University

In-person: Sofia Ortega-University of Wisconsin, John Hall-University of Idaho, Rebecca (Becky) Poole- TAMU, Jeremy Block-University of Wyoming, Sarah McCoski-University of Montana, Brenda Alexander-University of Wyoming, David Grieger-Kansas State University, Andrea Cupp-University of Nebraska, Ryan Ashley-New Mexico State University, Tod Hansen-Colorado State University, Milan Shipka-University of Alaska, Leslie Edgar-New Mexico State University, Jessica Nora Drum-SDSU, John Stevens-Utah State University, Rocio Rivera-University of Missouri

8:30 am – Business meeting called to order by Sofia Ortega

• 2022 meeting minutes approval- Ky Pohler moved to approve, Andrea Cupp second.
• Location for 2024 meeting- Tod said Reno (2 to 4 votes), Andrea said Madison (majority votes). The 2024 meeting will be in Madison, Wisconsin.
• New officer election- Becky Poole is the Secretary in 2023 and Chair in 2024. David Grieger will be Secretary in 2024 and Chair in 2025. Andrea moved for Jessica Nora Drum to be Member at Large and Sofia second. Jessica will be Secretary in 2025 and Chair in 2026.
• Station updates- Started at 8:45 am and ended at 9:38 am
• Break at 9:40 am

10:00 am – USDA update with Mark Mirando and Kamilah Grant

10:55 am – Station reports (asked to keep to approximately 15 minutes each) by Andrea Cupp-University of Nebraska, John Stevens-Utah State University, Caleb Lemley- Mississippi State University, John Hall-University of Idaho

12:00 pm – Adjourn for lunch.

1:00 pm – Station reports continued by Tod Hansen-Colorado State University, Jessica Nora Drum-SDSU, Karl Kerns-Iowa State University, David Grieger-Kansas State University (led to an open discussion on teaching methods to engage all types of undergraduate students including pre-vet, production, etc. and ensure student learning), Rocio Rivera-University of Missouri, Ryan Ashley-New Mexico State University

2:55 pm – Meeting adjourned for the day for Alaska Agriculture Tour and Salmon Bake dinner.

Tuesday, May 23, 2023

In attendance (New)

Virtual: Rick McCosh-Colorado State University

8:35 am – Impact statement workshop with Sarah Delheimer via Zoom

10:00 am – Station reports continued by Sarah McCoski-University of Montana, Becky Poole- TAMU, Jeremy Block and Brenda Alexander-University of Wyoming, Sofia Ortega-University of Wisconsin

11:30 am – Worked on impact statements for the annual report

            Gamete/Ovulation/Fertilization: KS, NE, CA, CO, IA, TX, NM – Lead KS

            Embryo Development/Recognition: UT, FL, CO, WI, WY, MT, SD – Lead WY

            Placentation/Fetal Development: MO, TX, NM, MS – Lead NM

            Postnatal Outcomes: CO, ID, MS – Lead ID

Meeting adjourned at 12:48 pm

Accomplishments

Short-term outcomes:

1. Established best practices for the training and evaluation of interdisciplinary statisticians, especially those working with agricultural collaborators. Utah
2. We empowered researchers to better design studies in order to achieve best statistical power in generalized linear mixed models.Utah
3. This project also furthered a collaboration within the W-4112 group. An MS student has completed her research in the NM station and now will be entering a Ph.D. program with W4112 member Sofia Ortega (WI station). New Mexico, Wisconsin

Outputs from the group during this reporting period:

1. 80 peer-reviewed manuscripts
2. 67 published abstracts
3. 13 Invited oral presentations
4. 8 theses/dissertations.

Activities

Objective 1

1. We are working on the refinement of tissue cryopreservation and culture techniques that will improve the prospects of using this as a tool for fertility preservation and efficiently tap into the abundant ovarian reserve of immature oocytes, improving the efficiency of assisted reproduction in cattle. California
2. We found evidence of the presence of neurotransmitters and their synthesis and metabolism in the bovine conceptus, which could have greater implications in establishing postnatal offspring behavior. Colorado
3. We learned that bulls differentially capacitate and release zinc ions in response to the concentration of bicarbonate present. This is important for the development of translational reproductive biotechnologies to analyze sire fertility potential using economical methods. Iowa
4. We showed that the zinc ion localization patterns exist in additional ruminants – goats. This could further our understanding of goat fertility. Iowa
5. We characterized a mechanism to identify potentially differentially expressed genes between low- and high-quality bovine oocytes. Utah
6. We established that cumulus cells of the ovarian follicle generate non-erythroid hemoglobin that is transported into the oocyte through transzonal projections. This non-erythroid hemoglobin seems to have at least an antioxidant role that may protect the oocyte and subsequent early embryo should fertilization occur from genotoxic stress. Wyoming
7. PRAMEY may be used as a biomarker for sperm quality and sperm function. Pennsylvania

 Objective 2

8. Understanding the roles that FSH may play in the development of preantral follicles is of critical importance as many ovarian stimulation protocols used in assisted reproduction use the hormone to promote antral follicle growth. California
9. We partially confirmed our hypothesis of an association of cytological endometritis and DMI and lactation performance. Additionally, cytological endometritis at both 15 DIM and at 30 DIM was associated with days to first ovulation, and further studies should now investigate this association with subsequent reproductive outcomes (i.e., pregnancy), taking into consideration the cows’ intake. Illinois
10. Identification of metabolic pathways disrupted in the d14 conceptus were identified in beef heifers exposed to a reduced plane of nutrition. These pathways may be used for targeted supplementation protocols in heifers during the post-AI feedlot to range transition to improve pregnancy retention. Montana
11. The open cow test is promising. Testing will continue in clinical trials while improvements in both the level of detection and method of sample collection will continue. Colorado
12. We learned that IL-1β could have potential as a therapeutic treatment for enhancing uterine receptivity and improving preimplantation embryo development and survival. Wyoming
13. We could determine that post-natal growth and development of calves can be programmed by exposure to choline during the preimplantation period. Wyoming
14. Understanding the neural mechanisms for the generation of the preovulatory GnRH/LH surge may enable future technologies to improve fertility and may provide insight to how fertility is suppressed during stress. Colorado
15. Understanding cells that make up CL will allow for the development of organoid structures that can be used in vitro to understand signal transduction and metabolic mechanisms critical for luteal function. Nebraska
16. Identifying sheep that also have High A4 populations indicates that this phenomena of naturally occurring androgen excess mammalians may be more wide-spread and that they all can be utilized as models for women who have been diagnosed with Polycystic ovary syndrome (PCOS). Nebraska
17. Understanding how the elimination of VEGFA may affect other hormones (AMH) and follicular development and reserve are critical to elucidating VEGFA’s function in the granulosa cells. Nebraska

 Objective 3

18. Identification of genetic markers associated with fertility, in addition to efforts to improve our understanding of the regulation of maternal recognition of pregnancy and embryo survival. Colorado
19. Improving our understanding of how BVDV programs the fetal immune system and impacts postnatal responses to secondary infections may help in identifying pregnancies and post-natal calves at risk as well as developing therapeutic approaches to control the infection. Colorado
20. We showed that paternal factors at estrus, such as treating embryo recipient cows with pooled seminal plasma, decreased embryo size, increased uterine artery resistance index, and decreased birth weights. Mississippi
21. Comparative animal models, such as cow and sheep, provide additional insight into the importance of glucose, fructose, minerals, and amino acids on the development of the conceptus and how dietary supplementation may enhance reproductive performance and successful outcomes of pregnancy and neonatal survival. Texas
22. Increased understanding of interferon tau-induced cell signaling may provide greater insight into pregnancy recognition signaling, as well as effects of interferon tau and progesterone/progestamedins to enhance transport of nutrients into the uterine lumen that are essential for establishment and maintenance of pregnancy. Texas
23. Increased understanding of prostaglandin function in the developing pregnancy. Texas
24. Increased understanding of the maternal vs paternal genome contributions to pregnancy and pregnancy loss. Texas
25. We characterized the various methods currently used for dealing with high missingness and zero inflation in single-cell RNA-seq data. Utah
26. Suppressing CXCL12-mediated signaling during implantation induces enduring placental effects manifesting later in gestation, highlighting the importance of this chemokine axis during implantation and placentation. New Mexico
27. Determined phenotypes to identify sires with potential fertility problems and developed a system to screen and identify sires of high and low fertility before they are released to the field which could improve overall herd fertility and profitability. Wisconsin
28. We are working on improving embryo development in vitro through cell culture modifications. The addition of FLI has been shown to improve embryonic development, cryotolerance, and pregnancy rates. By improving the IVP system, we can improve technology adoption by end users. Wisconsin 
29. We have validated a candidate mutation responsible for the Holstein Fertility Haplotype“HH2”. This provides us with insights into gene function and embryo development. Moreover, by knowing the exact mutation we can phase it out of the population by selective breeding. Wisconsin 
30. Identified methods/approaches to mitigate pregnancy complications associated with LOS and enhance producer profitability. Missouri

1. Abedal-Majed M.A., Abuajamieh M., Al-Qaisi M., Sargent K.M., Titi H.H., Alnimer M.A., Abdelqader A., Shamoun A.I. & Cupp A.S. (2023) Sheep with ovarian androgen excess have fibrosis and follicular arrest with increased mRNA abundance for steroidogenic enzymes and gonadotropin receptors. J Anim Sci 101.
2. Abedal-Majed M.A., Springman S.A., Jafar H.D., Bell B.E., Kurz S.G., Wilson K.E. & Cupp A.S. (2022a) Naturally occurring androgen excess cows are present in dairy and beef herds and have similar characteristics to women with PCOS. J Anim Sci 100.
3. Abedal-Majed M.A., Springman S.A., Sutton C.M., Snider A.P., Bell B.E., Hart M., Kurz S.G., Bergman J., Summers A.F., McFee R.M., Davis J.S., Wood J.R. & Cupp A.S. (2022b) VEGFA165 can rescue excess steroid secretion, inflammatory markers, and follicle arrest in the ovarian cortex of High A4 cowsdagger. Biol Reprod 106, 118-31.
4. Amaral T.F., Gonella-Diaza A., Heredia D., Melo G.D., Estrada-Cortes E., Jensen L.M., Pohler K. & Hansen P.J. (2022) Actions of DKK1 on the preimplantation bovine embryo to affect pregnancy establishment, placental function, and postnatal phenotypedagger. Biol Reprod 107, 945-55.
5. Ault-Seay T.B., Moorey S.E., Mathew D.J., Schrick F.N., Pohler K.G., McLean K.J. & Myer P.R. (2023) Importance of the female reproductive tract microbiome and its relationship with the uterine environment for health and productivity in cattle: A review.
6. Baskaran P., Mohandass A., Gustafson N., Bennis J., Louis S., Alexander B., Nemenov M.I., Thyagarajan B. & Premkumar L.S. (2023) Evaluation of a polymer-coated nanoparticle cream formulation of resiniferatoxin for the treatment of painful diabetic peripheral neuropathy. Pain 164, 782-90.
7. Beiki H., Murdoch B.M., Park C.A., Kern C., Kontechy D., Becker G., Rincon G., Jiang H., Zhou H. & Thorne J. (2022) Functional genomics of cattle through integration of multi-omics data. bioRxiv, 2022.10. 05.510963.
8. Botigelli R.C., Guiltinan C., Arcanjo R.B. & Denicol A.C. (2023) In vitro gametogenesis from embryonic stem cells in livestock species: recent advances, opportunities, and challenges to overcome. J Anim Sci 101.
9. Brown W., Oliveria M., Silva R.R., Demetrio D. & Block J. (2022a) 133 Effect of administration of mycobacterium cell wall fraction during the periovulatory period on the proportion of pregnancies obtained in virgin dairy heifers receiving in vitro-produced embryos. Reproduction, Fertility and Development 35, 194-.
10. Brown W., Oliveria M., Silva R.R., Demetrio D. & Block J. (2022b) 202 Effects of administration of mycobacterium cell wall fraction during follicle superstimulation on oocyte numbers and embryo development following ovum pickup and in vitro embryo production in virgin dairy heifers. Reproduction, Fertility and Development 35, 230-.
11. Butler M.L., Hartman A.R., Bormann J.M., Weaber R.L., Grieger D.M. & Rolf M.M. (2022) Genome-wide association study of beef bull semen attributes. BMC Genomics 23, 74.
12. Camacho L.E., Davis M.A., Kelly A.C., Steffens N.R., Anderson M.J. & Limesand S.W. (2022) Prenatal Oxygen and Glucose Therapy Normalizes Insulin Secretion and Action in Growth-Restricted Fetal Sheep. Endocrinology 163.
13. Camargo L.S.A., Saraiva N.Z., Oliveira C.S., Carmickle A., Lemos D.R., Siqueira L.G.B. & Denicol A.C. (2022) Perspectives of gene editing for cattle farming in tropical and subtropical regions. Anim Reprod 19, e20220108.
14. Carmickle A.T., Larson C.C., Hernandez F.S., Pereira J.M.V., Ferreira F.C., Haimon M.L.J., Jensen L.M., Hansen P.J. & Denicol A.C. (2022) Physiological responses of Holstein calves and heifers carrying the SLICK1 allele to heat stress in California and Florida dairy farms. J Dairy Sci 105, 9216-25.
15. Carvalho R.S., Cooke R.F., Cappellozza B.I., Peres R.F.G., Pohler K.G. & Vasconcelos J.L.M. (2022) Influence of body condition score and its change after parturition on pregnancy rates to fixed-timed artificial insemination in Bos indicus beef cows. Anim Reprod Sci 243, 107028.
16. Castillo-Salas C.A., Luna-Nevarez G., Reyna-Granados J.R., Luna-Ramirez R.I., Limesand S.W. & Luna-Nevarez P. (2023) Molecular markers for thermo-tolerance are associated with reproductive and physiological traits in Pelibuey ewes raised in a semiarid environment. Journal of Thermal Biology 112, 103475.
17. Clay C.M., Cherrington B.D. & Navratil A.M. (2021) Plasticity of Anterior Pituitary Gonadotrope Cells Facilitates the Pre-Ovulatory LH Surge. Frontiers in Endocrinology 11.
18. Contreras-Correa Z.E., Cochran T., Metcalfe A., Burnett D.D. & Lemley C.O. (2022) Seasonal and temporal variation in the placenta during melatonin supplementation in a bovine compromised pregnancy model. J Anim Sci 100.
19. Contreras-Correa Z.E., Messman R.D., Swanson R.M. & Lemley C.O. (2023) Melatonin in Health and Disease: A Perspective for Livestock Production. Biomolecules 13.
20. Davenport K.M., Ortega M.S., Liu H., O'Neil E.V., Kelleher A.M., Warren W.C. & Spencer T.E. (2023) Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta. Proc Natl Acad Sci U S A 120, e2221526120.
21. Dickson M.J., Bishop J.V., Hansen T.R., Sheldon I.M. & Bromfield J.J. (2022) The endometrial transcriptomic response to pregnancy is altered in cows after uterine infection. PLoS One 17.
22. Geisler S. (2022) Power Approximations for Generalized Linear Mixed Models in R Using Steep Priors on Variance Components.
23. Georges H.M., Van Campen H., Bielefeldt-Ohmann H. & Hansen T.R. (2022) Epigenomic and Proteomic Changes in Fetal Spleens Persistently Infected with Bovine Viral Diarrhea Virus: Repercussions for the Developing Immune System, Bone, Brain, and Heart. Viruses 14.
24. Goyal D., Limesand S.W. & Goyal R. (2023) Vascular Stem Cells and the Role of B-Raf Kinase in Survival, Proliferation, and Apoptosis. International Journal of Molecular Sciences 24.
25. Griffith E.H., Sharp J.L., Bridges W.C., Craig B.A., Hanford K.J. & Stevens J.R. (2022) The academic collaborative statistician: Research, training and evaluation. Stat 11.
26. Guadagnin A.R., Fehlberg L.K., Thomas B., Sugimoto Y., Shinzato I. & Cardoso F.C. (2022) Effect of feeding rumen-protected lysine through the transition period on postpartum uterine health of dairy cows. Journal of Dairy Science 105, 7805-19.
27. Haimon M., Estrada-Cortés E., Amaral T., Jeensuk S., Block J., Heredia D., Venturini M., Rojas C.S., Gonella-Diaza A. & DiLorenzo N. (2021) 1 Culture with choline chloride programs development of the in vitro-produced bovine embryo to increase postnatal bodyweight, growth rate, and testes size. Reproduction, Fertility and Development 35, 125-.
28. Halloran K.M., Stenhouse C., Moses R.M., Kramer A.C., Sah N., Seo H., Lamarre S.G., Johnson G.A., Wu G. & Bazer F.W. (2023) The ovine conceptus utilizes extracellular serine, glucose, and fructose to generate formate via the one carbon metabolism pathway. Amino Acids 55, 125-37.
29. Halloran K.M., Stenhouse C., Moses R.M., Seo H., Johnson G.A., Wu G. & Bazer F.W. (2022) Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation perioddagger. Biol Reprod 106, 865-78.
30. Harman A.R., Contreras-Correa Z.E., Messman R.D., Swanson R.M. & Lemley C.O. (2023) Maternal nutrient restriction and dietary melatonin alter neurotransmitter pathways in placental and fetal tissues. Placenta 131, 13-22.
31. Holton M.P., de Melo G.D., Dias N.W., Pancini S., Lamb G.C., Pohler K.G., Mercadante V.R.G., Harvey K.M. & Fontes P.L.P. (2022) Evaluating the use of luteal color Doppler ultrasonography and pregnancy-associated glycoproteins to diagnose pregnancy and predict pregnancy loss in Bos taurus beef replacement heifers. J Anim Sci 100.
32. Jauregui E.J., McSwain M., Liu X., Miller K., Burns K. & Craig Z.R. (2023) Human relevant exposure to di-n-butyl phthalate tampers with the ovarian insulin-like growth factor 1 system and disrupts folliculogenesis in young adult mice. bioRxiv.
33. Jeensuk S., Ortega M.S., Saleem M., Hawryluk B., Scheffler T.L. & Hansen P.J. (2022) Actions of WNT family member 5A to regulate characteristics of development of the bovine preimplantation embryodagger. Biol Reprod 107, 928-44.
34. Kern C., Wu W., Lu C., Zhang J., Zhao Y., Ocon-Grove O.M., Sutovsky P., Diaz F. & Liu W.S. (2023) Role of the bovine PRAMEY protein in sperm function during in vitro fertilization (IVF). Cell Tissue Res 391, 577-94.
35. Kern C.H., Feitosa W.B. & Liu W.S. (2022) The Dynamic of PRAMEY Isoforms in Testis and Epididymis Suggests Their Involvement in Spermatozoa Maturation. Frontiers in Genetics 13, 846345.
36. Kuzniar M., White R., Bromfield J. & Block J. (2022) 91 Treatment of bovine endometrial explants with interleukin-1 beta increases the relative abundance of transcripts for pro-inflammatory cytokines. Reproduction, Fertility and Development 35, 172-.
37. Lawlor M., Zigo M., Kerns K., Cho I.K., Easley C.A. & Sutovsky P. (2022) Spermatozoan Metabolism as a Non-Traditional Model for the Study of Huntington's Disease. International Journal of Molecular Sciences 23.
38. Liebig B.E., Bishop J.V., McSweeney K.D., Van Campen H., Gonzalez-Berrios C.L., Hansen T.R. & Thomas M.G. (2022) Direct genomic value daughter pregnancy rate and services per conception are associated with characteristics of day-16 conceptuses and hormone signaling for maternal recognition of pregnancy in lactating Holstein cows. Applied Animal Science 38, 157-69.
39. Lindsey M., Liu Y., Cuthbert J., Stevens J. & Isom C. (2022) 213 Using mRNA from cytoplasmic biopsies to assess molecular maturation and developmental potential of bovine oocytes. Reproduction, Fertility and Development 35, 235-6.
40. Makhijani R.B., Bartolucci A.F., Pru C.A., Pru J.K. & Peluso J.J. (2023) Nonerythroid hemoglobin promotes human cumulus cell viability and the developmental capacity of the human oocyte. F S Sci 4, 121-32.
41. McDonnell S.P., Candelaria J.I., Morton A.J. & Denicol A.C. (2022) Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration. J Vis Exp.
42. McIntosh S.Z., Quinn K.E. & Ashley R.L. (2022) CXCL12 May Drive Inflammatory Potential in the Ovine Corpus Luteum During Implantation. Reproductive Sciences 29, 122-32.
43. Moses R.M., Halloran K.M., Stenhouse C., Sah N., Kramer A.C., McLendon B.A., Seo H., Johnson G.A., Wu G.Y. & Bazer F.W. (2022a) Ovine conceptus tissue metabolizes fructose for metabolic support during the peri-implantation period of pregnancy(dagger). Biology of Reproduction 107, 1084-96.
44. Moses R.M., Kramer A.C., Seo H., Wu G., Johnson G.A. & Bazer F.W. (2022b) A Role for Fructose Metabolism in Development of Sheep and Pig Conceptuses. Adv Exp Med Biol 1354, 49-62.
45. Nogueira E., Tirpak F., Hamilton L.E., Zigo M., Kerns K., Sutovsky M., Kim J., Volkmann D., Jovine L., Taylor J.F., Schnabel R.D. & Sutovsky P. (2022) A Non-Synonymous Point Mutation in a WD-40 Domain Repeat of EML5 Leads to Decreased Bovine Sperm Quality and Fertility. Frontiers in Cell and Developmental Biology 10, 872740.
46. Ortega M.S., Bickhart D.M., Lockhart K.N., Null D.J., Hutchison J.L., McClure J.C. & Cole J.B. (2022a) Truncation of IFT80 causes early embryonic loss in Holstein cattle associated with Holstein haplotype 2. J Dairy Sci 105, 9001-11.
47. Ortega M.S., Rizo J.A., Drum J.N., O'Neil E.V., Pohler K.G., Kerns K., Schmelze A., Green J. & Spencer T.E. (2022b) Development of an Improved in vitro Model of Bovine Trophectoderm Differentiation. Frontiers in Animal Science 3, 49.
48. Pendleton A.L., Limesand S.W. & Goyal R. (2023) In Vivo Real-Time Study of Drug Effects on Carotid Blood Flow in the Ovine Fetus. J Vis Exp.
49. Perry G., Menegatti Zoca S., Walker J., Kline A.C., Andrews T.N., Rich J.J., Epperson K.M., Drum J.N., Ortega M.S. & Cushman R.A. Relationship of field and in vitro fertility of dairy bulls with sperm parameters, including DAG1 and SERPINA5 proteins. Frontiers in Animal Science 4, 58.
50. Plewes M.R., Przygrodzka E., Monaco C.F., Snider A.P., Keane J.A., Burns P.D., Wood J.R., Cupp A.S. & Davis J.S. (2023) Prostaglandin F2alpha regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum. Life Sci Alliance 6.
51. Poole R.K., Pickett A.T., Oliveira Filho R.V., de Melo G.D., Palanisamy V., Chitlapilly Dass S., Cooke R.F. & Pohler K.G. (2023) Shifts in uterine bacterial communities associated with endogenous progesterone and 17beta-estradiol concentrations in beef cattle. Domest Anim Endocrinol 82, 106766.
52. Posont R.J., Most M.S., Cadaret C.N., Marks-Nelson E.S., Beede K.A., Limesand S.W., Schmidt T.B., Petersen J.L. & Yates D.T. (2022) Primary myoblasts from intrauterine growth-restricted fetal sheep exhibit intrinsic dysfunction of proliferation and differentiation that coincides with enrichment of inflammatory cytokine signaling pathways. J Anim Sci 100.
53. Pru J.K. (2022) Pleiotropic Actions of PGRMC Proteins in Cancer. Endocrinology 163.
54. Reed S.A., Ashley R., Silver G., Splaine C., Jones A.K., Pillai S.M., Peterson M.L., Zinn S.A. & Govoni K.E. (2022a) Maternal nutrient restriction and over-feeding during gestation alter expression of key factors involved in placental development and vascularization. J Anim Sci 100.
55. Reed S.A., Balsbaugh J., Li X., Moore T.E., Jones A.K., Pillai S.M., Hoffman M.L., Govoni K.E. & Zinn S.A. (2022b) Poor maternal diet during gestation alters offspring muscle proteome in sheep. J Anim Sci 100.
56. Reese S.T., Franco G.A., de Melo G.D., Oliveira Filho R.V., Cooke R.F. & Pohler K.G. (2022) Pregnancy maintenance following sequential induced prostaglandin pulses in beef cows. Domest Anim Endocrinol 80, 106724.
57. Reid D.S., Geary T.W., Zezeski A.L., Waterman R.C., Van Emon M.L., Messman R.D., Burnett D.D. & Lemley C.O. (2023) Effects of prenatal and postnatal melatonin supplementation on overall performance, male reproductive performance, and testicular hemodynamics in beef cattle. J Anim Sci 101.
58. Rowell J.E., Blake J.E., Roth K.M., Sutton C.M., Sachse C.C., Cupp A.S., Geary T.W., Zezeski A.L., Alexander B.M., Ziegler R.L. & Shipka M.P. (2022) Medroxyprogesterone acetate in reindeer bulls: testes histology, cfos activity in the brain, breeding success, and semen quality. J Anim Sci 100.
59. Rule D.C., Melson E.A., Alexander B.M. & Brown T.E. (2022) Dietary Fatty Acid Composition Impacts the Fatty Acid Profiles of Different Regions of the Bovine Brain. Animals (Basel) 12.
60. Sah N., Stenhouse C., Halloran K.M., Moses R.M., Seo H., Burghardt R.C., Johnson G.A., Wu G. & Bazer F.W. (2022a) Inhibition of SHMT2 mRNA translation increases embryonic mortality in sheepdagger. Biol Reprod 107, 1279-95.
61. Sah N., Stenhouse C., Halloran K.M., Moses R.M., Seo H., Burghardt R.C., Johnson G.A., Wu G.Y. & Bazer F.W. (2022b) Creatine metabolism at the uterine-conceptus interface during early gestation in sheep. Biology of Reproduction 107, 1528-39.
62. Sharif M., Hickl V., Juarez G., Di X., Kerns K., Sutovsky P., Bovin N. & Miller D.J. (2022) Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans. Sci Rep 12, 6446.
63. Silva F., Camacho L.E., Lemley C.O., Hallford D.M., Swanson K.C. & Vonnahme K.A. (2022) Effects of nutrient restriction and subsequent realimentation in pregnant beef cows: Maternal endocrine profile, umbilical hemodynamics, and mammary gland development and hemodynamics. Theriogenology 191, 109-21.
64. Silva F., Martins T., Sponchiado M., Rocha C.C., Pohler K.G., Penagaricano F. & Binelli M. (2023) Hormonal profile prior to luteolysis modulates the uterine luminal transcriptome in the subsequent cycle in beef cross-bred cows section sign. Biol Reprod.
65. Smith B.D., Poliakiwski B., Polanco O., Singleton S., de Melo G.D., Muntari M., Oliveira Filho R.V. & Pohler K.G. (2022) Decisive points for pregnancy losses in beef cattle. Reprod Fertil Dev 35, 70-83.
66. Smith B.I. & Govoni K.E. (2022) Use of Agriculturally Important Animals as Models in Biomedical Research. Adv Exp Med Biol 1354, 315-33.
67. Sosa F., Uh K., Drum J.N., Stoecklein K.S., Davenport K.M., Ortega M.S., Lee K. & Hansen P.J. (2023) Disruption of CSF2RA in the bovine preimplantation embryo reduces development and affects embryonic gene expression in utero. Reprod Fertil 4.
68. Spooner-Harris M., Kerns K., Zigo M., Sutovsky P., Balboula A. & Patterson A.L. (2023) A re-appraisal of mesenchymal-epithelial transition (MET) in endometrial epithelial remodeling. Cell Tissue Res 391, 393-408.
69. Stegemiller M.R., Ellison M.J., Hall J.B., Sprinkle J.E. & Murdoch B.M. (2021) Identifying genetic variants affecting cattle grazing behavior experiencing mild heat load. Translational Animal Science 5, S61-S6.
70. Stenhouse C., Halloran K.M., Hoskins E.C., Newton M.G., Moses R.M., Seo H., Dunlap K.A., Satterfield M.C., Gaddy D., Johnson G.A., Wu G., Suva L.J. & Bazer F.W. (2022a) Effects of exogenous progesterone on the expression of mineral regulatory molecules by ovine endometrium and placentomesdagger. Biol Reprod 106, 1126-42.
71. Stenhouse C., Halloran K.M., Moses R.M., Seo H., Gaddy D., Johnson G.A., Wu G., Suva L.J. & Bazer F.W. (2022b) Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signalingdagger. Biol Reprod 106, 888-99.
72. Stenhouse C., Halloran K.M., Tanner A.R., Suva L.J., Rozance P.J., Anthony R.V. & Bazer F.W. (2022c) Uptake of Phosphate, Calcium, and Vitamin D by the Pregnant Uterus of Sheep in Late Gestation: Regulation by Chorionic Somatomammotropin Hormone. International Journal of Molecular Sciences 23.
73. Stenhouse C., Newton M.G., Halloran K.M., Moses R.M., Sah N., Suva L.J. & Bazer F.W. (2023) Phosphate, calcium, and vitamin D signaling, transport, and metabolism in the endometria of cyclic ewes. J Anim Sci Biotechnol 14, 13.
74. Stenhouse C., Seo H., Wu G., Johnson G.A. & Bazer F.W. (2022d) Insights into the Regulation of Implantation and Placentation in Humans, Rodents, Sheep, and Pigs. Adv Exp Med Biol 1354, 25-48.
75. Stenhouse C., Suva L.J., Gaddy D., Wu G. & Bazer F.W. (2022e) Phosphate, Calcium, and Vitamin D: Key Regulators of Fetal and Placental Development in Mammals. Adv Exp Med Biol 1354, 77-107.
76. Stoecklein K., Garcia-Guerra A., Duran B.J., Prather R. & Ortega M.S. Actions of FGF2, LIF, and IGF1 on Bovine Embryo Survival and Conceptus Elongation following Slow-rate Freezing. Frontiers in Animal Science, 134.
77. Swanson R., Contreras-Correa Z., Dinh T., King H., Sidelinger D., Burnett D. & Lemley C. (2022) Melatonin Supplementation Alters Maternal and Fetal Amino Acid Concentrations and Placental Nutrient Transporters in a Nutrient Restriction Bovine Model. Metabolites 12.
78. Wu G., Bazer F.W., Satterfield M.C., Gilbreath K.R., Posey E.A. & Sun Y. (2022) L-Arginine Nutrition and Metabolism in Ruminants. Adv Exp Med Biol 1354, 177-206.
79. Zhu C., Jiang Z., Johnson G.A., Burghardt R.C., Bazer F.W. & Wu G. (2022) Nutritional and Physiological Regulation of Water Transport in the Conceptus. Adv Exp Med Biol 1354, 109-25.
80. Zigo M., Kerns K., Sen S., Essien C., Oko R., Xu D. & Sutovsky P. (2022) Zinc is a master-regulator of sperm function associated with binding, motility, and metabolic modulation during porcine sperm capacitation. Communications Biology 5, 538.