Brief Summary of Minutes of Annual Meeting

Cotter

Objective 2. The demonstration of heterogeneity among plasmacyte series, cells known for antibody secretion, and the capacity to recognize differences among primitive PC and derived types is an important step in understanding the complexities of immune reactions. Recognition of Tϋrk cells as members of the avian proplasmacyte cell series adds to basic immune function.

Song

Objective 1. Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek’s disease using next generation sequencing.

Objective 3. The Epigenetics and Plasticity of CD4+ T Cells in Poultry Health

Broadbent

Objective 1. Evaluation of how different inbred lines of chickens with different allelic variation respond to very virulent (vv) infectious bursal disease virus (IBDV) infection.

Objective 2. Identify the molecular basis underpinning IBDV-mediated dysfunction and pathology of poultry immune system.

Objective 3. Development of new tools for IBDV research.

Gallardo

Objective 1. A comprehensive multilocus genomic analysis to compare DMV/1639 and QX strains. The role of maternal antibodies and early vaccination in the development of false layer syndrome. Testicular atrophy and epididymitis-orchitis associated with infectious bronchitis in broiler breeder roosters. Genotipic classification of avibacterium paragallinarum the causative agent of infectious coryza. Antigenic cartography as a tool to determine antigenic relatedness of avian reovirus variants.

Erf

Objective 2. Evaluation of the local (GF-pulp) cellular- and systemic (blood) antibody-responses to a first and second administration of autogenous Salmonella vaccines and vaccine components revealed a heterophil dominated, Th17-like, primary and secondary responses at site of intradermal injection. Analysis of SE-specific antibody profiles revealed classic primary and secondary response, with isotype switching to IgG and memory phenotype. Studies on multifactorial, non-communicable disease using the Smyth, UCD200/206 and OS autoimmune disease models, provided new insights into autoimmune pathology and revealed aberrant innate immune responses in the UCD-scleroderma model.

Objective 3. AR maintained and reproduced genetic lines that spontaneously develop autoimmune diseases. Refined and expanded the use of the growing feather as an in vivo test-tube system to study innate and adaptive immune responses in poultry.

Zhou

Objective 1. Improving food security in Africa by enhancing resistance to Newcastle disease virus and heat stress in chickens

Objective 2. Longitudinal Analysis of CD4 and CD8 T Cell Receptor Repertoires Associated with Newcastle Disease Virus Infection in Layer Birds

Jarosinski

Objective 1: N/A. Objective 2: Identification and characterization of chicken complement receptor-like 1 (CR1L) or complement component 4 binding protein, GPI-anchored (C4BPG) showed MDV gC interacts with CR1L in co-localization and co-immunoprecipitation assays.

Objective 3: We have cloned the putative chicken CR1, CR2, C3, and C4 with the goal of generating reagents such as mAbs.

Taylor

Objective 1.  Individual and pooled samples from chickens with defined alloantigen genotypes underwent SNP analyses.  Alloantigen I was associated with a region on chromosome 23.  alloantigen I was identified as RHCE as this candidate gene had high consistency between amino acid changing SNP and allelic differences. Alloantigen frequencies for the A, E, B, D, and I alloantigen systems were tested in two pair of lines divergently selected for antibody response against sheep red blood cells. Wageningen lines from generation 32 were control (C), high antibody (HA) and low antibody. Virginia Tech lines from generation 48 were high antibody (HAS) and low antibody (LAS). Altered frequencies for alloantigens A, E, B, and D were found between both sets of high antibody (HA, HAS) lines versus their corresponding low antibody (LA, LAS) lines. The distribution for alloantigen I differed between the Virginia Tech HAS vs LAS lines only.

Objective 3. Two inbred lines, four congenic lines and four line crosses typed at the major histocompatibility complex and other alloantigen systems are maintained for station research and collaboration.

Lamont

Objective 1. Bioinformatic and laboratory analyses demonstrated an important role of RIP2 in cellular response to APEC in HD11 cells.

Objective 3. ISU chicken genetic lines were reproduced and maintained and shared.

Drechsler

Objective 1. Developing project on Cluster Homolog Immunoglobulin-like Receptors in the chicken (CHIR). Phylogenetic analysis and re-annotation of CHIR submitted to NCBI. Preliminary data with siRNA shows effects on ChIR-B. Single-cell sequencing of the shell gland show differences in cellular populations and CHIRs in B2 and B19 haplotypes.

Objective 3: Continuation of functional annotation of the chicken genome: 20 tissues/cells in progress. DNA methylome completed for reproductive and intestinal tissues/ peripheral immune cells. RNA seq was completed for all tissues and peripheral blood cells. ATAC seq is ongoing with a new methodology for better quality and smaller cell populations due to issues with QC previously. ChIP seq with new methodology is ongoing for several tissues/cells.

Arsenault

Objective 2. From our acute Salmonella study we determined a number of novel insights into bacterial pathology (1) Salmonella is recognized by both TLR and NOD receptors that initiated the innate immune response; (2) activation of the PPRs induced the production of chemokines CXCLi2 (IL-8) and cytokines IL-2, IL-6, IFN-α, and IFN-γ; (3) Salmonella infection targeted the JAK-STAT pathway as a means of evading the host response by targeting the dephosphorylation of JAK1 and TYK2 and STAT1,2,3,4, and 6; (4) apoptosis appears to be a host defense mechanism where the infection with Salmonella induced both the intrinsic and extrinsic apoptotic pathways; and (5) the T cell receptor signaling pathway activates the AP-1 and NF-κB transcription factor cascades, but not NFAT. In our butyrate study we identified a novel and potential key mechanism of host response alteration due to butyrate. OCR and ECAR measurements showed that treatment with butyrate followed by Salmonella infection no difference in OCR of uninfected cells treated with SB compared to control. The increase in ECAR in butyrate treated cells is a sign of a pro-inflammatory response. In the reserpine study, reserpine treatment led to phosphorylative changes in epidermal growth factor receptor (EGFR), mammalian target of rapamycin (mTOR), and the mitogen-associated protein kinase 2(MEK2). Exogenous norepinephrine treatment alone increased Salmonella resistance, and reserpine-induced antimicrobial responses were blocked using beta-adrenergic receptor inhibitors, suggesting norepinephrine signaling is crucial in this mechanism. Overall, this study demonstrated a central role for MEK1/2 activity in reserpine induced neuro-immunometabolic signaling and subsequent antimicrobial responses in the chicken intestine, providing a means of reducing bacterial colonization in chickens to improve food safety.

Nazmi

Objective 3. Thus, the objective of this study is to characterize the intestinal intraepithelial lymphocytes (IELs) during the Eimeria infection. In the current study, at 14 day of age, SPF chicks were divided into 3 groups (n=30 each): non-infected control, and 2 infected groups (low-dose and high-dose). The low-dose group was challenged orally with 10000 oocysts/ml per bird, while the high-dose group was challenged with 20000 oocysts/ml per bird of Eimeria acervulina. A single cell suspension was prepared from duodenums collected from 8 bird/group at 2-days post-infection (dpi), 7dpi and 14dpi. Cells were stained with antibody cocktail and acquired with a flow cytometry. The number of IEL subpopulations including, TCRγδ, TCRβ, TCRneg, TCRβ+CD4+, TCRβ+CD4+CD8α+, TCRβ+CD8αβ+, TCRβ+CD8αα+, and iCD8α were significantly increased in the infected groups at 14dpi compared control group. However, there was no difference among groups at 2dpi and 7dpi. In addition, more challenge experiments with other Eimeria species will be tested.

Koci

Objective 3. Our team has developed a novel reporter plasmid which will be used to create a series of reporter cell lines, each containing a different immune related transcriptional response element (TRE) derived from avian genes. Our initial work has characterized our new plasmid which contains 2 reporter proteins. One under the control of a constitutive promoter and will serve as a control. The second is under the control of different TREs, and who’s activity will be induced by pathway specific stimuli. We have demonstrated the function of the positive and negative control plasmids. Furthermore, we have developed and initially characterized inducible reporter activity using the avian specific interferon stimulated response element (ISRE) sequence. We are currently in the process of bioinformatically identifying avian consensus TREs for multiple immune related transcription factors. Specifically: NFkB, AP-1, GAS, T-bet, STAT4, STAT6, GATA3, STAT3, FOXP3 SMAD, and GRE.

Parcells

Objective 2. We cloned the chicken EZH2 gene (2 isoforms) and the chicken SATB1 and have found that these interact with Meq splice variant-encoded proteins. These data have direct implications regarding the suppression of MDV lytic gene expression, transformation and the Treg patterning of MDV-latently-infected cells. We found that the long form of Meq in CVI988 actually confers higher levels of oncogenicity to RB-1B, but that the short form of this same Meq is attenuating. We also found that Meq isoforms from higher virulence strains have increased interactions with DNA-repair and transcriptional efficiency-regulating proteins, suggesting that MDV evolution of virulence may involve increased somatic mutation and or higher order chromatin structural regulation, as well as transcriptional regulation.

Hauck

Objective 2. Coccidia are among the most important intestinal pathogens in chickens. ARV are one of the possible reasons of runting-stunting syndrome. ARV are also known to be immunosuppressive, most likely by causing lymphoid depletion of immune organs. There are indications that co-infections with coccidia and avian reoviruses act synergistically. We plaque purified an ARV isolated from clinical cases or arthritis tenosynovitis in broiler chickens. We used this isolate and a laboratory strain in an experiment to establish a model for oral infections of chickens with ARV testing two different doses of each isolate. Samples taken at different time points are currently processed to characterize the expression of a panel of immune genes, lesions in various organs and the intestinal microbiota.

Dalloul

Objective 2.  Necrotic enteritis, is one of the major enteric diseases that negatively impacts the poultry industry.  The increasing ban on the use of antibiotic growth promoters in poultry production has resulted in higher incidence of necrotic enteritis outbreaks worldwide.  Previous research demonstrated that supplementation of natural additives led to unique microbiome signature accompanied by better performance and reduced pathology of broilers.  The current studies further dissected the host response during NE leading to new potential markers of disease progression that could be exploited in designing mitigation methods. 

Objective 3. Histomoniasis (aka blackhead disease) is a perennial problem in the poultry industry particularly turkeys where it inflicts substantial losses in poults as well as in broiler breeders.  We established a unique research model that closely resembles commercial field conditions and affords a much-needed opportunity for conducting detailed research on histomoniasis.  The newly established lateral transmission model in floor pens is a key system to study this disease, its progression, and potential mitigation strategies.

Peer Reviewed Publications

Asfor A, Nazki S, Reddy VRAP, Campbell E, Dulwich KL, Giotis ES, Skinner MA, Broadbent AJ. Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo. Viruses, 2021, 13(5), 933; doi: 10.3390/v13050933

Aylward, B.A., Johnson, C.N., Perry, F., Whelan, R., Zhang, C., Arsenault, R.J. Broiler chickens with 1950s genetics display a more stable immune profile as measured by kinome, mRNA expression, microbiome and metabolism when stimulated early in life with CpG. 2022. Poultry Science. 101(5), p.101775.

Bai H, He Y, Ding Y, Chu Q, Lian L, Heifetz EM, Yang N, Cheng HH, Zhang H, Chen J, *Song  Genome-wide characterization of copy number variations in the host genome in genetic resistance to Marek's disease using next-generation sequencing. BMC Genet. 2021 Jul 16;21(1):77. DOI: 10.1186/s12863-020-00884-w.

Blue, C.E.C, N.K. Emami, M.B. White, O. Gutierrez, S. Cantley, and R.A. Dalloul. 2022. Inclusion of Clarity Q manages growth performance, immune response, and nutrient transports of broilers during subclinical necrotic enteritis. Under review.

Blue, C.E.C, N.K. Emami, M.B. White, E. Kimminau, and R.A. Dalloul. 2022. Assessing the effects of a proprietary phytogenic feed additive on broilers during a necrotic enteritis challenge. Under review.

Botchway ,P.K., Amuzu-Aweh, E.N., Naazie, A., Aning, G. K., Otsyina, H.R., Saelao, P., Wang, Y., Zhou, H., Walugembe, M., Dekkers, J., Lamont, S.J., Gallardo, R.A., Kelly, T.R., Bunn, D. and Kayang, B.B. 2022. Host response to successive challenges with lentogenic and velogenic Newcastle disease virus in local chickens of Ghana. Poultry Science 101:102138. doi.org/10.1016/j.psj.2022.102138

Campbell E, Reddy VRAP, Gray A, Skinner M, Jennifer Simpson, Pippa Hawes, Broadbent AJ. Discrete virus factories form in the cytoplasm of cells co-infected with two strains of the segmented dsRNA virus, infectious bursal disease virus (IBDV), that subsequently coalesce. Journal of Virology, 2020, Jun 16; 94 (13), e-02107-19, doi: 10.1128/JVI.02107-19.

Coe, C., T. Boltz, R. Stearns, P. Foster, R. L. Taylor, Jr., J. S. Moritz, J. Jaczynski, A. Freshour, and C. Shen. 2022. Thermal inactivation of Salmonella typhimurium and the surrogate Enterococcus faecium in mash broiler feed in a laboratory scale circulated thermal bath. Poult. Sci. 101:101976 https://doi.org/10.1016/j.psj.2022.101976

Cotter, P. F., 2021a. Erythroplastids of duck blood produced by cytokinesis, lysis, and amitosis J. World Poult. Res. 11(2): 271-277. DOI: https://dx.doi.org/10.36380/jwpr.2021.32

Cotter, P. F., 2021b. Atypical hemograms of the commercial duck, Poult. Sci.100:2021,101248, ISSN 0032-5791, https://doi.org/10.1016/j.psj.2021.101248

Da Silva, A.P., R. Jude, R.A. Gallardo. Infectious bronchitis virus: A comprehensive multilocus genomic analysis to compare DMV/1639 and QX strains. Viruses. 2022.

Dulwich KL, Gray A, Asfor A, Giotis S, Skinner M, Broadbent AJ. The stronger downregulation of in vitro and in vivo innate antiviral responses by a very virulent strain of infectious bursal disease virus (IBDV), compared to a classical strain, is mediated, in part, by the VP4 protein. Frontiers in Cellular and Infection Microbiology, 2020, June 9, 10.315. doi: 10.3389/fcimb.2020.00315

Egaña-Labrin, S., C. Jerry, H. J. Roh, A. P. da Silva, C. Corsiglia, B. Crossley, D. Rejmanek, R. A. Gallardo. Avian Reoviruses of the Same Genotype Induce Different Pathology in Chickens. Avian Diseases. 2022.

Figueroa, A., E. Escobedo, M. Solis, C. Rivera, A. Ikelman and R.A. Gallardo. Outreach Efforts to Prevent Newcastle Disease Outbreaks in Southern California. Viruses. 2022

Emami, N.K., and R.A. Dalloul. 2021.  Centennial Review: Recent developments in host-pathogen interactions during necrotic enteritis in poultry. Poultry Science 100:101330.

Emami, N.K., A.L. Fuller, and R.A. Dalloul. 2022.  Lateral transmission of Histomonas meleagridis in turkey poults raised on floor pens.  Poultry Science 101:101951.

Fulton. J. E. W. Drobik-Czwarno, A. Wolc, A. M. McCarron, A.R. Lund, C. J. Schmidt and R. L. Taylor, Jr. 2022.  The chicken A and E blood group systems are due to variation in proteins encoded by genes within the chicken RCA syntenic gene region. J. Immunol. 209: 1-10 https://doi.org/10.4049/jimmunol.2101010

Gallardo, R.A. Molecular Characterization of Variant Avian Reoviruses and its Relationship with Antigenicity and Pathogenicity. Avian Diseases. 2022.

Gallardo, R.A., and A.P. Da Silva. MHC B Complex Genetic Resistance and Immune Responses to Infectious Bronchitis Virus in Chickens. Avian Diseases. 2022.

Gallardo, R.A., da Silva, A.P., Gilbert, R., Alfonso, M., Conley, A., Jones, K., Stayer, P.A. and Hoerr, F.J., 2022. Testicular Atrophy and Epididymitis-Orchitis Associated with Infectious Bronchitis Virus in Broiler Breeder Roosters. Avian Diseases, 66(1), pp.112-118.

Gilbert, I. M, J. M. Santamaria, and G. F. Erf. 2022. Time-course investigation of dermal leukocyte response to lipoteichoic acid in chickens.  Discovery 22:44-50.

Jiang J, Chen C, Cheng S, Yuan X, Jin J, Zhang C, Sun X, Song J, Zuo Q, Zhang Y, Chen G, Li B. Long Noncoding RNA LncPGCR Mediated by TCF7L2 Regulates Primordial Germ Cell Formation in Chickens. Animals (Basel). 2021 Jan 24;11(2):292. DOI: 10.3390/ani11020292. PMID: 33498947; PMCID: PMC7912682.

Jing, Y., Yuan, Y., Monson, M. Wang, P., Mu, F., Zhang, Q., Na, W., Zhang, K., Wang, Y., Leng, L., Li, Y., Luan, P., Wang, N., Guo, R., Lamont, S., Li, H., and Yuan, H. 2022. Multi-omics association reveals the effects of intestinal microbiome-host interactions on fat deposition in broiler lines divergently selected for abdominal fat content. Frontiers in Microbiology 12:815538. doi: 10.3389/fmicb.2021.815538

Kaiser, M., Hsieh, J., Kaiser, P. and Lamont, S.J. 2022. Differential immunological response detected in mRNA expression profiles among diverse chicken lines in response to Salmonella challenge. Poultry Sci. 101: 101605 https://doi.org/10.1016/j.psj.2021.101605

Kogut, M., Genovese, K.J., Byrd, J.A., Swaggerty, C., He, H., Farnell, Y., Arsenault, R. Chicken-Specific Kinome Analysis of Early Host Immune Signaling Pathways in the Cecum of Newly Hatched Chickens Infected with Salmonella enterica Serovar Enteritidis. 2022. Frontiers Cellular and Infection Microbiology. 857.

Krieter A, Xu H, Akbar H, Kim T, Jarosinski KW*. 2022. The conserved Herpesviridae protein kinase (CHPK) of Gallid alphaherpesvirus 3 (GaHV3) in required for horizontal spread and natural infection in chickens. Viruses 14(3):586. https://doi.org/10.3390/v14030586

Meyer, M.M., Lamont, S.J., and Bobeck, E.A. 2022. Mitochondrial and glycolytic capacity of peripheral blood mononuclear cells isolated from diverse poultry genetic lines: optimization and assessment. Frontiers in Veterinary Sci. 8:815878. doi: 10.3389/fvets.2021.815878

Montine, P., T.R. Kelly, S. Stoute, A.P. da Silva, B. Crossley, C. Corsiglia, H.L. Shivaprasad, and R.A. Gallardo. Infectious Bronchitis Virus Surveillance in Broilers in California (2012-2020). Avian Diseases. 2022.

Nguyen, Veronica, Asli Mete, Anibal Armien, Ana P. da Silva, Patrick Montine, Charles Corsiglia, VM Sadagopa Ramanujam, Karl E. Anderson, Ruediger Hauck, and Rodrigo A. Gallardo. Porphyrin Accumulation and Biliary Lithiasis Causing Diffusely Black Livers in Broiler Chickens. Avian Diseases 66, no. 2 (2022): 1-5.

Reddy VRAP, Nazki S., Brodrick A.J., Asfor A., Urbaniec J., Morris Y., Broadbent A. J. Evaluating the breadth of neutralizing antibody responses elicited by infectious bursal disease virus (IBDV) genogroup A1 strains using a novel chicken B-cell rescue system and neutralization assay. Journal of Virology, 2022, Sep 7;e0125522.doi: 10.1128/jvi.01255-22

Reddy VRAP, Campbell EA, Wells J, Simpson J, Nazki S, Hawes PC, Broadbent AJ. Birnaviridae virus factories show features of liquid-liquid phase separation, and are distinct from paracrystalline arrays of virions observed by electron microscopy. Journal of Virology, 2022, Feb 9;jvi0202421. doi: 10.1128/jvi.02024-21.

Redweik, G.A.J., Kogut, M.H., Arsenault, R.J., Lyte, M., Mellata, M. Reserpine improves Enterobacteriaceae resistance in chicken intestine via neuro-immunometabolic signaling and MEK1/2 activation. 2021. Communications Biology, 4(1), 1-11

Rocchi, A., J. Ruff, C. J. Maynard, A. J. Forga, R. Señas-Cuesta, E. S. Greene, J. D. Latorre, C. N. Vuong, B. D. Graham, X. Hernandez-Velasco, G. Tellez Jr., V. M. Petrone-Garcia, B. M. Hargis, G. F. Erf, C. M. Owens, and G. Tellez-Isaias. 2022. Cyclic heat stress model alters intestinal permeability, bone mineralization, and meat quality in broiler chickens. Animals 12:1273 doi: 10.3390/ani12101273.

Sato J, Murata S, Yang Z, Kaufer BB, Fujisawa S, Seo H, Maekawa N, Okagawa T, Konnai S, Osterrieder N, Parcells MS, Ohashi K. Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek's Disease Virus on Transactivation Activity and Virulence. Viruses. 2022 Feb 14;14(2):382. doi: 10.3390/v14020382. PMID: 35215975; PMCID: PMC8876991.

Sherer ML, Lemanski EA, Patel RT, Wheeler SR, Parcells MS, Schwarz JM. A Rat Model of Prenatal Zika Virus Infection and Associated Long-Term Outcomes. Viruses. 2021 Nov 18;13(11):2298. doi: 10.3390/v13112298. PMID: 34835104; PMCID: PMC8624604.

Song, J. He, Y, Ding, Y. Tian, F. Zhao, K., Zhang, H., Yu, Y., Yang, N., Lian, L., Luo, J., Mitra, A. The Epigenetics and Plasticity of CD4+ T Cells in Poultry Health, Journal of Animal Science, Volume 99, Issue Supplement_3, November 2021, Page 55, https://doi.org/10.1093/jas/skab235.098

Sorrick, J., W. Huett, K. A. Byrne, and G. F. Erf. 2022. Autoimmune activities in choroids of visually impaired Smyth chickens with autoimmune vitiligo. Front. Med. 9:846100. doi:10.3389/fmed.2022.846100.

Sun C, Jin K, Zuo Q, Sun H, Song J, Zhang Y, Chen G, Li B. Characterization of Alternative Splicing (AS) Events during Chicken (Gallus gallus) Male Germ-Line Stem Cell Differentiation with Single-Cell RNA-seq. Animals (Basel). 2021 May 20;11(5):1469. doi: 10.3390/ani11051469. PMID: 34065391; PMCID: PMC8160964.

Sun C, Jin K, Zhou J, Zuo Q, Song J, Yani Z, Chen G, Li B. Role and function of the Hintw in early sex differentiation in chicken (Gallus gallus) embryo. Anim Biotechnol. 2021 Jun 21:1-11. DOI: 10.1080/10495398.2021.1935981. Epub ahead of print. PMID: 34153202.

Sun, H., Yang, Y., Cao, Y., Li, H., Qu, L., Lamont, S.J. 2022. Gene expression profiling of RIP2-knockdown in HD11 macrophages—elucidation of potential pathways (gene network) when challenged with avian pathogenic E. coli (APEC) BMC Genomics 23 (1), 1-20. doi.org/10.1186/s12864-022-08595-5

Sun, H., Li, N., Tan, J., Li, H., Zhang, J., Qu, L., Lamont, S.J. 2022. Transcriptional regulation of RIP2 gene by NFIB is associated with cellular immune and inflammatory response to APEC infection Int. J. Mol. Sci. 23(7):3814. doi.org/10.3390/ijms23073814

Swaggerty, C.L., Byrd, J.A., Arsenault, R.J.,  Perry, F., Johnson, C.N., Genovese, K.J., He, H., Kogut, M.H., Piva, A., and Grilli, E. A blend of microencapsulated organic acids and botanicals reduces necrotic enteritis via specific signaling pathways in broilers. 2022. Poultry Science. p.101753.

Taylor, R. L., Jr. 2022.  Nunc Dimitis – Fred M. McCorkle, Jr. Poult. Sci. 101:101854 https://doi.org/10.1016/j.psj.2022.101854

Taylor, R. L., Jr. 2022.  The 50 most downloaded articles from Poultry Science in 2021. Poult. Sci. 101: 101818 https://doi.org/10.1016/j.psj.2022.101818

Tudeka, C.K., Aning, G.K., Naazie, A., Botchway, P.K., Amuzu-Aweh, E.N., Agbenyegah, G.K., Enyetornye, B., Fiadzomor, D., Saelao, P., Wang, Y., Kelly, T.R., Gallardo, R., Dekkers, J.C.M., Lamont, S.J., Zhou, H., and Kayang, B.B. 2022. Response of three local chicken ecotypes of Ghana to lentogenic and velogenic Newcastle disease virus challenge. Tropical Animal Health and Production 54:134. doi.org/10.1007/s11250-022-03124-8

Xu H, Krieter AL, Ponnuraj N, Tien YY, Kim T, Jarosinski KW*. 2022. Coinfection in the host can result in functional complementation between live vaccines and virulent virus. Virulence 13(1);980. https://doi.org/10.1080/21505594.2022.2082645

Zhang, C., Zuo, Q., Wang, M., Chen, H., He, N., Jin, J., Li, T., Jiang, J., Yuan, X., Li, J., Shi, X., Zhang, M., Bai, H., Zhang, Y., Xu, Q., Cui, H., Chang, G., Song, J., Sun, H., Zhang, Y., Chen, G., and Li, B. (2021) Narrow H3K4me2 is required for chicken PGC formation. J Cell Physiol 236, 1391-1400

Zhang, J., R. M. Goto, C. F. Honaker, P. B. Siegel, R. L. Taylor, Jr., H. K. Parmentier, and M. M. Miller. 2022.  Association of MHCY genotypes in lines of chickens divergently selected for high or low antibody response to sheep red blood cells. Poult. Sci. 101:101621 https://doi.org/10.1016/j.psj.2021.101621

Zhang, J., R. M. Goto, A. Psifidi, M. P. Stevens, R. L. Taylor, Jr., and M. M. Miller. 2022. Research Note: MHCY haplotype and Campylobacter jejuni colonization in a (Line N x Line 61) x Line N backcross population.  Poult. Sci. 101:101654 https://doi.org/10.1016/j.psj.2021.101654

Zhao R, Zuo Q, Yuan X, Jin K, Jin J, Ding Y, Zhang C, Li T, Jiang J, Li J, Zhang M, Shi X, Sun H, Zhang Y, Xu Q, Chang G, Zhao Z, Li B, Wu X, Zhang Y, Song J, Chen G, Li B. Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells. Nat Commun. 2021 May 20;12(1):2989. DOI: 10.1038/s41467-021-23242-5. PMID: 34017000; PMCID: PMC8138025.

Abstracts

Abraham, M., M. Erasmus, G. Fraley, G. F. Erf, and D. Karcher. 2022. Understanding stress and welfare of laying pullets using stock density and feeder space stressors. American Association of Pathologists.

Beck, C. N., J. Santamaria, M. A. Sales, and G. F. Erf. 2022. Primary and secondary immune responses in Light-brown Leghorn pullets vaccinated with Salmonella vaccines. International Poultry Scientific Forum, Atlanta January 2022. accepted

Beck, C. N., J. Santamaria, M. A. Sales, and G. F. Erf. 2022. Local mRNA expression of cytokines during the first seven days following the intradermal administration of autogenous Salmonella vaccines in previously vaccinated Light-brown Leghorn pullets. Poult. Sci. 101 (E-Suppl. 1).

Beck, C. N., J. Santamaria, M. A. Sales, and G. F. Erf. 2022. Local cellular- and systemic humoral-responses to intradermal injection of killed autogenous Salmonella vaccines in immunized and non-immunized Light-brown Leghorn pullets. International Avian Immunology Research Group Meeting, September 26-29, 2022, Newark, DE.

Blue CEC, Emami NK, White MB, Gutierrez O, Cantley S, and Dalloul RA. Effects of Quillaja saponaria extract on mRNA abundance of tight junction proteins and cellular metabolism genes during a necrotic enteritis challenge in broilers. International Poultry Scientific Forum. 2022.

Blue CEC, Emami NK, Gutierrez O, Cantley S, and Dalloul RA. Effects of Clarity Q on mRNA abundance of nutrient transporters during a subclinical necrotic enteritis. Poultry Science Association Annual Meeting. 2022.

Emami NK, and Dalloul RA. Comparison of two Clostridium perfringens strains for inducing subclinical necrotic enteritis in broiler chickens. International Poultry Scientific Forum. 2022.

Emami NK, Fenster DA, Blue CEC, and Dalloul RA. Differential analysis of breast muscle and liver mRNA in broiler chickens challenged with Eimeria maxima with/without Clostridium perfringens. World Poultry Congress. 2022.

Emami NK, Fuller AL, and Dalloul RA. Lateral Transmission of Histomonas meleagridis in turkey poults raised on floor pens. World Poultry Congress. 2022.

Froebel LE, Emami NK, and Dalloul RA. Evaluation of circulatory mRNA abundance of pro-inflammatory and regulatory cytokines and receptors during a subclinical necrotic enteritis challenge. International Poultry Scientific Forum. 2022.

Froebel LE, and Dalloul RA. Evaluating mRNA abundance of cytokines and chemokines in the modern broiler and heritage breed during a necrotic enteritis challenge. Poultry Science Association Annual Meeting. 2022.

Fulton. J. E. W. Drobik-Czwarno, A. Wolc, C. Schmidt, and R. L. Taylor, Jr. 2022.  Identification of the Genes Responsible for the Chicken A and E Blood Group Systems. Plant and Animal Genome PAG PAG XXIX 2022 https://pag.confex.com/pag/xxix/meetingapp.cgi/Paper/45612

Ng, TT, Hawkins, RD, and Drechsler, Y. An update on transcriptome of an array of chicken ovary, intestinal, and immune cells and tissues. Poultry Science Association. July 11th to 14th, 2022. San Antonio, Texas.

Patria, Joseph, Nirajan Bhandari, Phaedra Travlarides-Hontz, Benedikt B. Kaufer, and Mark S. Parcells. Marek’s disease virus (MDV) evolution of virulence: Investigating the selection for protein binding interfaces at the C-terminus of the Meq oncoprotein. Proceedings of 94th Annual Northeastern Conference on Avian Diseases (NECAD) Penn State University, Sept. 14 and 15, 2022 

Parcells, M.S., Katneni, U.K., Neerukonda, S., Tavlarides-Hontz, P., Arsenault, R.J. Cell culture and In Vivo Examination of the Mechanism of Action of Victrio a DNA-Liposome-based Innate Immune Agonist. 18th International Conference on Production Diseases in Farm Animals; 2022 June 15–17; Madison, WI

Perry, F., Bortoluzzi, C., Eyng, C., Aeschleman, L., Jones, E., Kogut, M., Arsenault, R., The immunometabolic effects of butyrate in chicken small intestines and macrophage-like cells. World Poultry Congress; 2022 August 8-11; Paris, France

Perry, F., Bortoluzzi, C., Eyng, C., Kogut, M., Arsenault, R. The immunometabolic effects of sodium butyrate supplementation in the ileum of broiler chickens. Poultry Science Association Annual Meeting; 2022 July 1-14; San Antonio, TX.

Perry F., Bortoluzzi, C., Lahaye, L., Santin, E., Johnson, C., Korver, D.R., Kogut, M.H., Arsenault R.J. Protected Biofactors and Antioxidants Reduce the Negative Consequences of Virus and Cold Challenge while Enhancing Performance by Modulating Immunometabolism through Cytoskeletal and Immune Signaling. Symposium on Gut Health in Production of Food Animals; 2021 November 1-3; St. Louis, MO.

Runcharoon K, Emami NK, and Dalloul RA. Differential analysis of autophagy-related genes in broilers challenged with Eimeria maxima with or without Clostridium perfringens. Poultry Science Association Annual Meeting. 2022.

Santamaria, J., C. N. Beck, M. A. Sales, and G. F. Erf. 2022. Local and systemic inflammatory and antibody responses to intradermal administration of killed Salmonella Vaccine in different vaccine vehicles. Poult. Sci. 101 (E-Suppl. 1).

Shaimaa K. Hamad, Shuja Majeed, Ali Nazmi. Characterization of Intestinal Immune Responses to Coccidiosis in Chicken. 2022 Food for Health Discovery Annual Meeting. Columbus OH

Sparling, B. and Drechsler, Y. Talk. An update on chicken transcriptome and epigenome annotation, factors involved in host resistance against IBV; and establishing Ig-like receptors' role in innate immunity. NE-1834 Genetic Bases for Resistance and Immunity to Avian Diseases. September 17-19, 2021. Baltimore, Maryland.

Sparling, B. and Drechsler, Y. Talk. Identification of immunoglobulin-like receptors in the chicken genome that are associated with disease resistance. January 8, 2022. Plant and Animal Genome Conference. San Diego, CA.

Sparling, B. and Drechsler, Y. Talk. Advances in identifying immunoglobulin-like receptors and their roles in immunity in the chicken. Western University of Health Sciences, College of Veterinary Medicine, 2022 CVM Research Day. March 28, 2022. Pomona, California.

Sparling, B. and Drechsler, Y. Talk. Determining chicken immunoglobulin-like receptors (CHIRs) expression and their effect on immune response in a macrophage disease model. American Association of Immunologists. May 6-9, 2022. Portland, Oregon.

Taylor, R. L., Jr., W. Drobik-Czwarno, and J. E. Fulton. 2022.  Chicken alloantigen D is CD99. Poult. Sci. 101: (E-Suppl 1) in press

Taylor, R. L., Jr., W. Drobik-Czwarno, and J. E. Fulton. 2022.  Identifying chicken alloantigens A, E and D. AIRG meeting in press

Book Chapters

Lamont, S.J., Dekkers, J.C.M., Wolc, A. and Zhou, H. 2022. Immunogenetics and the mapping of immunological functions. Pp. 277-297. In: Avian Immunology, 3rd ed. B. Kaspers, K.A. Schat, T. Goebel, L. Vervelde, Eds., Elsevier, London, San Diego, Cambridge, Oxford. Doi.org/10.1016/C2018-0-00454-5.

Thesis/Dissertation

Ye Bi, M.S. Animal Biology. Longitudinal Analysis of CD4 and CD8 T Cell Receptor Repertoires Associated with Newcastle Disease Virus Infection in Layer Birds

“The Regulation and Role of Glycoprotein C during Herpesvirus Pathogenesis.” Widaliz Vega Rodriguez, PhD Dissertation 2022. University of Illinois at Urbana-Champaign. Supervisor: Keith W. Jarosinski

EVALUATING THE ROLE OF IRG1 AND ITACONATE ON MAREK’S DISEASE VIRUS INFECTION, Kristy Wisser-Parker, MS in Biological Sciences (defense date June xx, 2022)

THE ROLE OF EXOSOMES IN MAREK’S DISEASE VIRUS VACCINE RESPONSE, Aksana Dallakoti, non-thesis MS in Bioinformatics (defense date August 22, 2022)

INFECTION DYNAMICS OF A CHICKEN T-CELL LINE BY DIFFERENT PATHOTYPES OF MAREK’S DISEASE VIRUS (MDV), Joshua Miller, MS in Biological Sciences (defense date August 26, 2022)

White, Mallory B.  In ovo and feed application of probiotics or synbiotics and response of broiler chicks to post-hatch necrotic enteritis. PhD Dissertation, August 2021.

Froebel, Laney E.  Evaluating immune related genes in heritage and modern broiler breeds and macrophages in response to clostridium perfringens. MS, July 2022.