SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: WERA89 : Potato Virus and Virus-Like Disease Management
- Period Covered: 10/01/2021 to 09/30/2022
- Date of Report: 06/16/2022
- Annual Meeting Dates: 03/15/2022 to 03/16/2022
Participants
On-line: David Douches Hanu Pappu Jason Ingram Jonathan Whitworth Joseph Coombs Kylie Swisher Grimm Max Feldman Melanie Filiatrault Steve Hystad Tami Brown Alan Westra Prabu Gnanasekaran Mathuresh Singh Richard Manasseh Hira Kamal Kiwamu Tanaka Gregory Elison Brooke Babler Rich Novy Noelle Anglin In-person: Adam Winchester Alexander Karasev Alice Pilgeram Amy Charkowski Andrew Houser Brian Charlton Erik Wenninger Jen Rushton John Mizicko Julie Pasche Kasia Duellman Kenneth Frost Kent Sather Lynn Woodell Mark Pavek Nathan Gelles Nina Zidack Nora Olsen Rabecka Hendricks Renee Rioux Sarah Hensley Teresa Almeida Vamsi Nalam Vidyasagar Sathuvalli Yuan Zeng In-person: Adam Winchester Alexander Karasev Alice Pilgeram Amy Charkowski Andrew Houser Brian Charlton Erik Wenninger Jen Rushton John Mizicko Julie Pasche Kasia Duellman Kenneth Frost Kent Sather Lynn Woodell Mark Pavek Nathan Gelles Nina Zidack Nora Olsen Rabecka Hendricks Renee Rioux Sarah Hensley Teresa Almeida Vamsi Nalam Vidyasagar Sathuvalli Yuan Zeng
Accomplishments
The group continued to conduct research, extension programs and other outreach related to potato viruses and virus-like organisms. For example:
An SCRI grant, led by Dr. Alex Karasev at the University of Idaho, was awarded in fall of 2021. Numerous collaborators across multiple states have made progress in four areas and continue efforts to develop management solutions for necrotic viruses (PMTV and PVY) that infect potato.
Work continues to understand the factors affecting the spread of PVY. Advances have been made in reducing virus levels in New Brunswick in recent years. Major trends in PVY spread were identified via amassing a very large data set of grower practices and other factors. Management decisions by individual growers play a large role, among other factors. Led by Dr. Mathuresh Singh, this effort may provide other regions with strategies to improve PVY management.
Research conducted in Idaho and New York to determine the interaction between cultivar and PVY strain in both the foliage and tubers will aid in certification efforts. A poster detailing the results is publicly available by contacting Jonathan Whitworth.
Dr. Hanu Pappu’s group is working on several aspects of PVY including virus-induced metabolic adjustments, PVY-coded NIa protein modulating the auxin-signaling pathway and disease development, and the roles of PMTV proteins using a PVX-based expression system.
Advances in dormant tuber testing for PVY (potato virus Y) continue. However, the industry is not yet at the point of replacing seed-lot grow outs, particularly since dormant tuber testing cannot efficiently detect chemical injury and variety mix. Continued advances in tuber testing methods will shift the metric of a good test beyond what has always been the first hurdle of the “false negative rate” and how we plan for beyond that. Stakeholders have requested more information that could be collected using expanded dormant tuber tests to aid in farm decision making.
SHERLOCK (specific high-sensitivity reporter unlocking) is being investigated for PVY detection. This method combines recombinase polymerase amplification (RPA) with a second by cleavage of a reporter probe by a Cas13 enzyme to reduce false negative results.
Environmental sensors are being investigated to improve the ability to predict powdery scab. Sensors were placed in commercial potato fields across five states and environmental / crop data were correlated to disease incidence and severity. Principle component analysis was used on soil characteristics. Root gall formation was correlated to root susceptibility and initial pathogen density.
Impacts
- Topic-oriented breakout sessions at the annual meeting allowed members to discuss and identify goals and objectives related to specific topics. The seed certification section discussion centered around winter testing sites, dormant tuber testing, greenhouse testing, and options for breaking dormancy. PVY, PMTV and PLRV were all discussed in the cultivar development and breeding section. Breeding and cultivar development are complicated by the nature of virus resistance and the many other selection traits important to the industry. Sustainability requirements may provide more push for virus resistance in the future. The PMTV section focused discussions on detection and the need for improved tools. Information is lacking in the distribution and diversity of PMTV and the Spongospora vector. Additionally, more work is needed in the area of soil and environmental characteristics, and viral etiology.
- State certification agency personnel from Oregon, Idaho, Montana, Wisconsin, Colorado, Nebraska, North Dakota, and New Brunswick (Canada) provided seed certification reports, indicating number of acres planted and accepted for certification and some challenges associated with the 2021 certification crops. Both decreases and increases in PVY incidence were observed, depending on growing region. Some states saw slight increases in certification acres while others saw decreases due to disease, access to water, and other factors.
- Presentations on current research were provided by attendees. Over-arching categories for updates included virus-like organisms, diagnostics, TRV/PMTV, PVY biology, PVY management, and other topics. These updates generated good discussion and showed progress in areas such as dormant tuber testing, sampling strategies, PVY strain composition and spread, PVY management with mineral oils and biologicals, tools to breed for resistance, variety reactions to PMTV, powdery scab/PMTV prediction modeling, and others.
Publications
Combest, M.M., Moroz N., Rogan C., Tanaka K., Anderson J., Rakotondrafara A.M., and Goyer A. 2021. StPIP1, a predicted PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with potato virus Y. Journal of Experimental Botany (In Press) https://academic.oup.com/jxb/advance-article-abstract/doi/10.1093/jxb/erab078/6157931
Dahan, J., Cooper, W.R., Munyaneza, J., and Karasev, A.V. (2022) A new Bactericera cockerelli picorna-like virus identified in populations of potato psyllids. Archives of Virology 167: 177-182 (https://doi.org/10.1007/s00705-021-05281-x).
Dahan, J., Wenninger, E.J., Thornton, M., Reyes-Corral, C.A., Olsen, N., and Karasev, A.V. (2021) Haplotyping the potato psyllid (Hemiptera: Triozidae), and the associated pathogenic bacterium ‘Candidatus Liberibacter solanacearum’ in non-crop alternative hosts in Southern Idaho. Environmental Entomology 50: 382–389 (https://doi.org/10.1093/ee/nvaa179).
Elison, G.L., Novy, R.G. & Whitworth, J.L. Russet Potato Breeding Clones with Extreme Resistance to Potato Virus Y Conferred by Rychc as well as Resistance to Late Blight and Cold-Induced Sweetening. Am. J. Potato Res. 98, 411–419 (2021). https://doi.org/10.1007/s12230-021-09852-1
Goyer A (2021) From tolerant to sensitive: how a small peptide produced by potato plants determines the fate of potato-PVY interaction. Potato Progress. Vol. XXI, Number 4.
Mondal, S., Ghanim, M., Roberts, A., and Gray, S. M. 2021. Different potato virus Y strains frequently co- localize in single epidermal leaf cells and in the aphid stylet. Journal of General Virology. 102:001576 DOI 10.1099/jgv.0.001576
Mondal, S., Wintermantel, W. M., and Gray, S. 2022. Infection dynamics of potato virus Y isolate combinations in three potato cultivars. Plant dis. https://doi.org/10.1094/PDIS-09-21-1980-RE
Olsen, N. and A. Karasev. 2021. Going viral- tackling the problem of evolving viruses. Potato Grower Magazine. May 50(5):36-37.
Olsen, N., A. Karasev, J. Miller and J. Whitworth. 2022. Everything you wanted to know about potato viruses, but were afraid to ask. Idaho Potato Conference. Pocatello, ID. January 19, 2022.
Olsen, N. 2021. Effective practices to manage Black Spot Bruise and PVY in potatoes and the proper use of products for good storage management CHS Bingham Cooperative Grower Meeting (virtual). March 4, 2021.
Olsen, N. and J. Miller. 2021. Powdery scab and potato mop-top virus (PMTV) management. Miller Research Potato Pest Management seminar (virtual). February 18, 2021.
Pandey, B., Mallik, I., Arjarquah, A., Shahoveisi, F., and Pasche, J. S. 2022. Assessing optimum timing for detecting Potato mop-top virus (PMTV) in potato seed tubers. Plant Dis. (abstract) in press.
Reyes-Corral, C., Cooper, W.R., Horton, D., Miliczky, E., Riebe, J., Waters, T., Wildung, M., and Karasev, A.V. (2021) Association of Bactericera cockerelli (Hemiptera: Triozidae) with the perennial weed Physalis longifolia (Solanales: Solanaceae) in the potato-growing regions of western Idaho. Environmental Entomology 50: 1416-1424
Rodriguez-Rodriguez, M., Quintero-Ferrer, A., Green, K.J., Robles-Hernandez, L., Gonzalez-Franco, A.C., and Karasev, A.V. (2021) Molecular and biological characterization of recombinant isolates of Potato virus Y circulating in potato fields in Mexico. Plant Disease 105: 2688-2696 (https://doi.org/10.1094/PDIS-10-20-2215-RE).
Ross, B.T., Zidack, N., and Flenniken, M.L., Extreme Resistance to Viruses in Potato and Soybean, (2021), Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.658981.
Ross, B.T., Zidack, N., McDonald, R., and Flenniken, M.L. 2022. Transcriptome and Small RNA Profiling of Potato Virus Y Infected Potato Cultivars, Including Systemically Infected Russet Burbank. Viruses. 14(3), 523; https://doi.org/10.3390/v14030523
Tran, L., K. Green, M. Rodriguez-Rodriquez, G. Orellana, C. Funke, O. Nikolaeva, A. Quintero-Ferrer, M. Chikh-Ali, L. Woodell, N. Olsen and A. Karasev. 2021 (online Oct 2021). Prevalence of recombinant strains of potato virus Y in seed potato planted in Idaho and Washington states between 2011 and 2021. Plant Disease. https://doi.org/10.1094/PDIS-08-21-1852-SR.
Vales, M.I., Scheuring, D.C., Koym, J.W. et al. Vanguard Russet: A Fresh Market Potato Cultivar with Medium-Early Maturity and Long Dormancy. Am. J. Potato Res. (2022). https://doi.org/10.1007/s12230-022-09877-0
Whitworth, J.L., Gray, S.M., Ingram, J.T. et al. Foliar and Tuber Symptoms of U.S. Potato Varieties to Multiple Strains and Isolates of Potato virus Y. Am. J. Potato Res. 98, 93–103 (2021). https://doi.org/10.1007/s12230-020-09820-1
Woodell, L. and N. Olsen. 2021. The impact of Tobacco Rattle Virus on Potato Quality at Harvest and in Storage. American Journal of Potato Research (abstract). In press.
Zhang, C., Zarka, K.A., Zarka, D.G. et al. Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties. Am. J. Potato Res. 98, 42–50 (2021). https://doi.org/10.1007/s12230-020-09815-y