Brief Summary of Minutes of Annual Meeting

1. Welcome and Introduction, Jeff Brecht, Chair
a. Attending: Eva Almenar, Bassam Annous, Jeff Brecht, Xuetong Fan, Hao Feng, Mabel Gil, Bob Houtz, Rama Nannapaneni, Steve Sargent, Keith Schneider, Angela Shaw, and Shecoya White.
b. Guests: Jinhe Bai (ARS-FL), Hongda Chen (NIFA), Cheryl Enlow (UFPA Food Safety & Technol. Council Chair), Xuetong Fan (ARS-PA), Jorge Fonseca (ARS-MD), Jim Gorny (FDA), Emily Griep (UFPA Mgr. Food Safety), Yaguang (Sunny) Luo (ARS-MD), Jennifer McEntire (UFPA VP Food Safety & Technol.), Anne Plotto (ARS-FL), Tianbao Yang (ARS-MD)
c. The agenda for the meeting was approved.
d. The minutes of the 2019 meeting were approved.

2. Report from the Administrative Advisor, Robert Houtz, Assoc. Dean for Research and Dir. of the Agric. Exp. Station at University of Kentucky. Dr. Houtz introduced himself and his background in horticulture. He reported that Eric Young, the regional Executive Director for SAESSD, is retiring and his replacement will be Gary Thompson from Penn State, who will be located in Arkansas. Otherwise, nothing is changed with regard to multistate, regional projects. Dr. Houtz mentioned that there is interest at USDA in direct marketing of produce such as CSAs, particularly with regard to food safety.

3. USDA NIFA Grant Opportunities, Dr. Hongda Chen, National Program Leader, USDA NIFA. Dr. Chen spoke about research priorities for produce researchers within the USDA NIFA grant program. Dr. Chen updated us on the changes occurring at NIFA related to the move to Kansas City. He also discussed research priorities, mentioning food safety and reduction of food losses, including nutritional losses, that are within the S294 scope.

4. Station Reports. Station reports were presented from U. Florida (Brecht & Sargent), Auburn (Brecht, for Woods), Iowa St. (Shaw), Michigan St. (Almenar), and Mississippi St. (Nannapaneni).

5. Improving S294 Engagement with United Fresh, Dr. Jennifer McEntire, VP Food Safety & Technology, United Fresh Produce Association. Dr. McEntire presented the research needs of the produce industry as seen by the UFPA membership, emphasizing food safety. She discussed how S-294 members can collaborate with United Fresh membership on research opportunities, mentioning that she is willing to write letters of support for research proposals if given sufficient time to interact with the researchers, such as giving feedback during proposal development. S294 member biosketches were shared with Dr. McEntire to facilitate more interaction.

6. Research Opportunities with FDA, Dr. Jim Gorny, Sr. Science Advisor, FDA. Dr. Gorny presented the produce-related research needs and opportunities as seen by FDA. He discussed how S-294 members can collaborate with FDA on research priorities. He explained that FDA does not fund outside research, but does sometimes form research partnerships with businesses and research institutions.

7. Station Reports (cont.). Station reports were presented from ARS-FL (Plotto), ARS-PA (Fan), and ARS-MD (Luo and Yang).

New Business:
1. Eva Almenar led a discussion on submission of a conference proposal to USDA. The idea is for S294 to sponsor a conference on cutting edge research in improving food safety and quality, to include new technology.
a. Need to address critical areas / topics of concern from a domestic and international perspective. Invite cutting edge researchers to represent each topic (TBD).
b. Include interaction between microbiological and plant physiology/food science in the research.
c. Inclusive participation, including students (scholarships/travel grants?) and underrepresented institutions.
d. Format, structure, presentation topics, target audience, and budget/funding were discussed. Discussion of the location centered on USDA ARS, Beltsville meeting facility, with the possibility of linking the timing to the UFPA annual Washington D.C. meeting.
e. An organizing committee to be chaired by Eva Almenar with members Brecht, Fonseca, Luo, Shaw, and possibly Woods (pending medical outcome) will meet and continue planning for a 2021 submission of a conference grant proposal to USDA.
2. Brecht brought up renewal of the current 5-year S294 project, which will expire in 2022. That means that the next S294 Project Statement should be submitted in 2021. He asked for volunteers to serve on the writing committee to email him.
3. No other business

Adjourn: Next meeting of S-294 meeting in Los Angeles, CA, 2021.

Objective 1. Evaluate methods of sampling and measuring flavor and nutrition of fresh-cut products to facilitate comparison to traditional shelf-life factors.
• Bai, J., Plotto, A. and Manthey, J. Distribution of volatile and the secondary metabolites in different fruit structures in tomato and citrus. Flavonoid glycosides are the most important bioactive compounds and are abundant in citrus albedo. Citrus juice or other products containing more albedo tissue usually are more health-beneficial but may sacrifice taste with enhanced bitterness. (ARS-FL).
• Shaw et al., focused on the quality and safety testing of value-added produce products (IA).
• Wilson’s team worked with 13 groups (13 Orchard Operators, 3 Entrepreneurs, and two companies) to test the pH, titratable acidity, degrees brix, color, water activity, density and composition of various jams, jellies, chocolate/fruit/ pepper blends, and syrups. These products required verification to ensure they meet both Federal (Food and Drug Administration) and State (Iowa Department of Inspections and Appeals) standards. All tests were performed in triplicate, and the results were reported and when appropriate to the Federal or state agency. Two groups had their kitchens certified, and two have contracted with co-packers to mass-produce their certified products. The products that passed inspection, were/are sold in farmers markets, and some regional grocery chains. Some groups had to resubmit samples, if their first or second or third set did not meet standards. This work is ensuring safe products are present within the local food markets (IA).

Objective 2. Develop new strategies to improve and maintain inherent fresh-cut product quality and nutrition.
• Plotto, A., Bai, J., Baldwin, E.A., Brecht, J.K. Overcoming limitations for processing riper mango fruit for fresh-cut mango. Mangoes should be conditioned (ripened) to a firmness level of 30-35N (6-8 lbs-force) (compression test) before cutting to achieve good eating quality and 10 days shelf life. Dipping mango slices in citric acid maintains good appearance in storage and prevents browning. (ARS-FL).
• Ritenour, M., Plotto, A., Bai, J. Plant essential oils to enhance decay control of Florida fresh strawberries Botrytis cinerea is the most common decay organism found on strawberries postharvest. Thymol or carvacrol are fungistatic in vitro. Application of plant essential oils in microencapsulated powder placed in a sachet on a clamshell lid controls decay without providing additional (off) flavor. (ARS-FL).
• Ritenour, M., Plotto, A., Bai, J. Developing effective pre- and postharvest methods to control Diplodia stem-end rot (Lasiodiplodia theobromae) and reduce mandatory segregation of fresh Florida grapefruit exports arriving in Japan. Project started 2019-2020 season. Establish Lasiodiplodia theobromae colonization level and other fungal population in selected Florida grapefruit groves. (ARS-FL).
• Brecht et al., developed recommendations for minimum firmness for processing different mango cultivars (Tommy Atkins, Keitt, Kent), with and without additives and MAP (FL).
• Brecht et al., reported that lower temperatures in enclosed refrigerated retail display cases maintain higher quality of leafy greens. An approximately 3 degree C temperature difference before versus after display case retrofitting resulted in improved quality maintenance of baby spinach, spring mix and Romaine lettuce; we also demonstrated lower microbiological populations on chopped Romaine lettuce (FL).
• Sargent et al., determined the quality evaluation of commercial broccoli cultivars and breeding selections for potential winter production in north Florida (part of East Coast Broccoli Project) was done. There were inherent differences between cultivars but cooling with slush ice or hydrocooling did not result in differences in quality changes during storage; plastic film overwrap was effective in reducing water loss during storage. Samples from broccoli storage regimes were analyzed for nutritional content at Univ. of Tennessee in Dr. Carl Sams' laboratory (FL).
• Belisle et al., demonstrated how trimming shanks better maintains total sugars and water content and reduces kernel denting of sweetcorn kernels during storage (FL).
• Sargent et al., began evaluation of a purple-fleshed sweet potato with potential as a high-value crop for Florida growers. We are testing effects of curing regime and harvest time on anthocyanin content and other quality factors during storage at 10C (FL).
• Shaw et al., the use of surrogate microorganisms that act like the human pathogens are critical for the fresh-cut industry to have an accurate model. The selection and evaluation of surrogate microorganism is an area that is under researched in the microbiology field. Drs. Wilson and Mendonca worked with a local grocery store to determine how fresh-cut cantaloupe melons would be affected by different simulated retail storage conditions. In this work utilized surrogate bacteria to mimic human pathogens. This work estimates the potential financial impact of up to $1.5 million in cost savings from reducing waste from out-of-date fresh-cut cantaloupe melon and fresh produce (IA).
• Shaw and Mendonca have been working to identify potential surrogate microorganisms that can be used within a produce field setting and in a fresh-cut processing setting. To date, these two labs have been able to identify 5 strains that can be utilized for microbial risk assessment that mimic the attributes of Escherichia coli O157:H7. As a result of this results, as of December 11th, 2019 these strains have been cited in 62 peer-reviewed journal articles as being used in other food safety research. With so few of options for surrogate available, this is a major contribution to the field of food safety (IA).
• da Rocha et al., Created a double-bottom antimicrobial package that extends apple shelflife (MI).
• da Rocha et al., demonstrated novel package slows Penicillium expansum growth (MI).
• da Rocha et al., reported novel package reduces apple ethylene production, respiration rate and firmness loss (MI).
• da Rocha et al., demonstrated double-bottom antimicrobial packages with ICsa extends apple shelf life better than with ICp (MI).
• da Rocha et al, reports new strategy to reduce postharvest losses in countries lacking cold storage (MI).
• Pranata et al., demonstrated that EWP film can be produced through extrusion and calendaring processes and can be used as an alternative to other materials for food packaging applications where thermal resistance, rigidity, strength, barrier to oxygen, and avoidance of condensation are desired (MI).
• Fehlberg et al., report eight different LLDPE/OP sheets from combinations of different OP characteristics are examined. Results show that OP particle sizes of 75-177 µm without zest at 0.4-g loading improve thermal stability, color, dispersion, tensile strength, modulus of elasticity, and elongation at break as well as reduce air bubble formation and OP agglomerate sizes in LLDPE/OP composites. Results show a promise in utilization of OP fillers to produce a composite comparable to neat LLDPE and composites with UV-blocking properties (MI).

Objective 3. Improve understanding of physiological mechanisms that affect fresh-cut product quality.

• Kargar Ph.D. project. Evaluating physiochemical properties and bioactive compounds of selected banana cultivars: Consumer demand, browning potential of fresh with intent to expand fresh-cut product. (AL).
• Shi, M.S. project. Evaluating impact of hydroponic production of lettuce-on-lettuce quality with emphasis on secondary metabolism. (AL).
• Evaluating interactive effects of environment, growing season and cultivar of blueberries destined for value-added consumer / homeowner quality. (AL).
• Evaluating combinational applications of labeled herbicides on hill and plasticulture for postharvest quality of selected cultivars destined for fresh-cut. (AL).
• Xia, M.S. project. Evaluating impact of hydroponic production of lettuce using nutrient film technique, incidence of tipburn and phenolic content. (AL).
• Belisle et al., are developing rapid quality assessments for breeding line selections and applying chemical interventions to reduce pink rib, a common physiological disorder of lettuce (FL).
• Wilson and Mendonca to evaluated how natural antimicrobials can be utilized to kill Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in juice products. In this research the essential oil component isoeugenol (at 0.5 μLmL−1) enhances the microbial safety of pineapple juice with an acceptable impact on the sensory attributes of the juice. This can serve as an alternative to heat pasteurization and meet consumer demand for minimally processed fresh-like juices (IA).
• Shaw has been focusing on how best to educate produce growers on the risk factors that impact the safety of the produce they grow, harvest, process, and distribute. In 2018/2019, six peer review articles were published on projects related to produce food safety. Additionally, there were 16 produce safety alliance grower trainings provided in Iowa to educate 430 produce growers and 10 on-farm food safety visits to produce farms. All of this research has been presented at conferences through the U.S. It is estimated that over 500 food industry, academics, and government agencies have been exposed to this research and extension publications. Knowledge assessments taken from the produce safety alliance grower trainings indicate a significant increase in knowledge related to the food safety modernization act produce safety rule (p<0.001) and changes in behavior were observed through on-farm visits at all 21 farms in 2019. These include adoption of increased documentation, employee training, facility upgrades and technology (IA).

Objective 4. Determine critical factors in controlled inoculation studies with human pathogens and surrogates that influence the outcome of quantitative microbial risk assessments.

• De J et al., determined the required concentrations of free chlorine in hydrocooling systems in order to reduce microbial load on whole sweetcorn (FL).
• Jayeola, et al., report, L. monocytogenes growth was more accurately predicted in celery (RMSE 0.28 to 0.47) than in onions (RMSE 0.42 to 1.53) under the fluctuating temperature scenarios tested. However, both predictions yielded APZ values, which ranged from 82 to 100% for celery and 36 to 78% for onions. In contrast, Salmonella Typhimurium populations increased < 1 log CFU/g in diced tomatoes under the three fluctuating temperature scenarios studied. Overall, these diced products packaged under a high oxygen atmosphere showed a decreased pathogen growth compared to product stored in a passive modified atmosphere. Findings from this study will be particularly useful in assessing the risk associated with consumption of diced celery, tomatoes and onions and in designing effective packaging strategies to minimize pathogen growth in fresh-cut produce (MI).
• Nannapaneni and White, reported that rugose morphotype of S. Typhimurium and S. Heidelberg produced significantly denser biofilm on food-contact surfaces which also increased with sequential exposure to sublethal concentrations of NaOCl in the case of S. Heidelberg and these biofilms were more tolerant to biocidal NaOCl concentrations commonly used in the food processing plants (MS).
• Nannapaneni and White, reported that oxidative based agents are the best choice to decontaminate the food contact surface where acid stress adapted L. monocytogenes cells may be present (MS).
• Nannapaneni and White, reported that cold stressed cells of L. monocytogenes Bug600 and ScottA have greater tolerance to lethal alkali and some oxidative stresses that are commonly employed by the food industries to kill this foodborne pathogen (MS).

Objective 5. Development and validation of novel diagnostic methods to determine presence of human pathogens and chemical hazards associated with fresh and fresh-cut products.

Nothing to report.

Publications
Kargar, M., F.M.Woods, M.M. Wall, R.J. Kessler, E. Vinson III, E.G. Fonsah, R.B. Jeganathan, and N. Larsen. 2019. Screening underutilized banana for carotenoid content: Consumer acceptance and potential health benefits. Journal of the American Pomological Society 73(4):198-205

Thomas-Popo, E., A. Mendonca, J. Dickson, A. Shaw, S. Coleman, A. Daraba, A. Jackson-Davis, and F. Woods. 2019. Isoeugenol significantly inactivates Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in refrigerated tyndallized pineapple juice with added Yucca schidigera extract. Food Control 106:106727

Baldwin, E.A. and J.K. Brecht. 2020. Advances in the use of barrier coatings and additives in the preservation of fresh horticultural produce, p. 91-115. In: C. Watkins (ed.). Advances in Postharvest Management of Horticultural Produce. Burleigh Dodds, Cambridge, U.K.

Belisle, C.E., S.A. Sargent, and J.K. Brecht. 2018. Trimming sweetcorn shanks better maintains total sugars and water content of kernels during storage. Proc. Fla. State Hort. Soc. 131:189-192

De, J., Y. Li, A. Sreedharan, R. Goodrich Schneider, A. Gutierrez, M. Jubair, M.D. Danyluk, K.R. Schneider. 2018. A three-year survey of Florida packinghouses to determine microbial loads on pre- and post-processed tomatoes. Food Control 86:383-388

Gurtler, J.B, N.A. Harlee, A.M. Smelser and K.R. Schneider. 2018. Salmonella enterica contamination of market fresh tomatoes: A review. J. Food Protect. 81(7):1193-1213 https://doi.org/10.4315/0362-028X.JFP-17-395

Bardsley, C.A., L.N. Truitt, R.C. Pfunter, M.D. Danyluk, S.L. Rideout, L.K. Shawn. 2019. Growth and survival of Listeria monocytogenes and Salmonella on whole and sliced cucumbers. J. Food. Prot. 82:301-309

Brecht, J.K., S.A. Sargent, P.E. Brecht, J. Saenz, and L. Rodowick. 2019. J.K. Brecht (ed.). Protecting Perishable Foods During Transport by Truck or Rail. University of Florida and USDA AMS, Gainesville, Fla., HS1328, 204 p.

De, J., A. Sreedharan, Y. Li, A. Gutierre, J.K. Brecht, S.A. Sargent, and K.R. Schneider. 2019. Comparing the efficacy of postharvest cooling methods to enhance fruit quality and reduce Salmonella in artificially inoculated southern highbush blueberry. HortTechnology 29:314-319

Plotto, A., J.K. Brecht, J. Bai, E. Baldwin, E. Branca, D. Wood., C. Abrahan, S. Stahl, and Y. Xie. 2019. Overcoming limitations for processing riper mango fruit for fresh–cut mango. Final Report to the National Mango Board

Schneider, K.R., D.L. Archer, R. Goodrich Schneider, G.L. Baker, M.D. Danyluk, and C. Thomas. 2019. The Food Recall Manual (Version 2). University of Florida, IFAS Press, Gainesville, FL.

Tokarskyy, O. and K.R. Schneider. 2019. Influence of temperature, humidity, and diluent type on survival of Salmonella spp. on the surface of raw tomatoes. Potravinarstvo. 13(1):325-330 https://doi.org/10.5219/1121

Wang, L., E.A. Baldwin, W. Luo, W. Zhao, J. Brecht, and J. Bai. 2019. Key tomato volatile compounds during postharvest ripening in response to chilling and pre-chilling heat treatments. Postharv. Biol. Technol. 154:11-20 https://doi.org/10.1016/j.postharvbio.2019.04.013

Brecht, J.K. 2020. Fresh-cut mango: Requirements and recommendations, p. 495-500. In: R. Beaudry and M.I. Gil (eds.). Controlled and Modified Atmosphere for Fresh and Fresh-Cut Produce. Academic.

Brecht, J.K. 2020. Fresh and fresh-cut sweetpotato: Requirements and recommendations, p. 629-633. In: R. Beaudry and M.I. Gil (eds.). Controlled and Modified Atmosphere for Fresh and Fresh-Cut Produce. Academic.

Brecht, J.K., E. Pliakoni, and K. Batziakias. 2020. The impact of temperature on atmosphere requirements and effects: The limits of design and utility for CA/MA/MAP, p. 147-166. In: R. Beaudry and M.I. Gil (eds.). Controlled and Modified Atmosphere for Fresh and Fresh-Cut Produce. Academic.

Brecht, J.K., I. Uysal, M. C. N. Nunes, J. P. Emond, S. Mercier, and U. McCarthy. 2020. Smart distribution to maintain shelf-life of horticultural produce, p. 409-432. In: C. Watkins (ed.). Advances in Postharvest Management of Horticultural Produce. Burleigh Dodds, Cambridge, U.K.

de Frias J.A., Y. Luo, B. Zhou, B. Zhang, D.T. Ingram, K. Vorst, J.K. Brecht, and J. Stommel. 2020. Effect of door opening frequency and duration of an enclosed refrigerated display case on product temperatures and energy consumption. Food Control 111:107044 (8 p.) https://doi.org/10.1016/j.foodcont.2019.107044

Wang, F., Mendonca, A., Brehm-Stecher, B., Dickson, J., Dispirito, A., and A. Shaw. 2018. Long-term-survival phase cells of Salmonella typhimurium ATCC 14028 have significantly greater resistance to ultraviolet radiation in 0.85% saline and apple juice. Foodborne Pathogens and Disease 15(9):538-543

Bai, J., Baldwin, E., Tsantili, E., Plotto, A., Sun, X., Wang, L., Kafkaletou, M., Wang, Z., Narciso, J., Zhao, W., Xu, S., Seavert, C., and Yang, W. 2019. Modified humidity clamshells to reduce moisture loss and extend storage life of small fruits. Food Packaging and Shelf Life 22:100376 https://doi.org/10.1016/j.fpsl.2019.100376

Bowman, K.D., McCollum, G., Plotto, A., and Bai, J. 2019. Minnie finger lime: a new novelty citrus cultivar. HortScience 54:1425-1428 https://doi.org/10.21273/HORTSCI13622-18

Whitaker, V.M., Peres, N.A., Osorio, L.F., Fan, Zhen, do Nascimento Nunes, M.C., Plotto, and A., Sims, C.A. 2019 ‘Florida Brilliance’ strawberry. HortScience 54:2073-2077 https://doi.org/10.21273/HORTSCI14327-19

Li, J., Di, T., and Bai, J. 2019. Distribution of volatile compounds in different fruit structures in four tomato cultivars. Molecules 24:2594-2019 https://doi.org/10.3390/molecules24142594

Sun, X.N., Baldwin, E.A., and Bai, J. 2019. Applications of gaseous chlorine dioxide on postharvest handling and storage of fruits and vegetables – A review. Food Control 95:18-26 https://doi.org/10.1016/j.foodcont.2018.07.044.

Sun, X.N., Cameron, R.G., and Bai, J. 2019. Microencapsulation and antimicrobial activity of carvacrol in a pectin-alginate matrix. Food Hydrocolloids. 92:69-73 https://doi.org/10.1016/j.foodhyd.2019.01.006.

Marín, A., Plotto, A., Atarés, L., and Chiralt, A. 2019. Lactic acid bacteria incorporated into edible coatings to control fungal growth and maintain postharvest quality of grapes. HortScience 54:337-343. https://doi.org/10.21273/HORTSCI13661-18

Endo, H., Ose, K., Bai, J., and Imahori,Y. 2019. Effect of hot water treatment on chilling injury incidence and antioxidative responses of mature green mume (Prunus mume) fruit during low temperature storage. Scientia Horticulturae 246:550-556. https://doi.org/10.1016/j.scienta.2018.11.015

Bhullar, M., Shaw, A., Hannan, J., and S. Andrews. 2019. Extending the holding time for agricultural water testing method EPA 1603 for produce growers. Water: 11 (10): 2020; https://doi.org/10.3390/w11102020

Shaw, A., and K. Helterbran. 2018. Development of a food safety training for prison farm: Challenges and opportunity. Journal of Extension: 56(7):7IAW8 https://joe.org/joe/2018december/iw8.php?utm_source=email&utm_medium=email&utm_campaign=2018december

Overdiep, J., and A. Shaw. 2019. Development of a FSMA human food audit checklist for food processors. Journal of Extension: 57 (3): 3TOT4 https://www.joe.org/joe/2019june/tt4.php

Gomez, C., Currey, C., Dickson, R., Kim, H., Hernández, R., Sabeh, N., Raudales, R., Brumfield, R., Laury-Shaw, A., Wilke, A., and S. Burnett. 2019. Controlled Environment Food Production for Urban Agriculture. HortScience: 54 (9):1448-1458.

Perry, B., Shaw, A., Johnsen, E., Enderton, A., Strohbehn, C., and L. Naeve. 2019. Assessment of midwest growers’ needs for compliance with The Food Safety Modernization Act Produce Safety Rule. Food Protection Trends: 39(3):212-217.

Strohbehn, C., Enderton, A., Shaw, A., B. Perry, Overdiep, J. and L. Naeve. 2019. What do growers’ need to comply with the FSMA Produce Safety Rule? Journal of Extension: 56(7):7RIB1 https://joe.org/joe/2018december/rb1.php

da Rocha Neto, A.C., Beaudry, R., Maraschin, M., Di Piero, R.M., and Almenar, E. 2019. Double-bottom antimicrobial packaging for apple shelf-life extension. Food Chemistry 279:379-388 https://doi.org/10.1016/j.foodchem.2018.12.021

Pranata, M.P., González-Buesa, J., Chopra, S., Kim, K., Pietri, Y., Ng, P.K.W., Matuana, L.M., and Almenar, E. 2019. Egg white protein film production through extrusion and calendering processes and its suitability for food packaging applications. Food and Bioprocess Technology 12:714-727. https://doi.org/10.1007/s11947-019-2248-0

Fehlberg, J., Lee, C-L., Matuana, L., Almenar, E. 2019. Orange peel waste from juicing as raw materials for plastic composites intended for use in food packaging. Journal of Applied Polymer Science 137:48841 https://doi.org/10.1002/app.48841

Jayeola, V., Jeong, S., Almenar, E., Marks, B., Vorst, K., Brown, W., and Ryser, E. 2019. Predicting the growth of Listeria monocytogenes and Salmonella typhimurium in diced celery, onions, and tomatoes during simulated commercial transport, retail storage, and display. Journal of Food Protection 82(2):287-300. https://doi.org/10.4315/0362-028X.JFP-18-277

Bansal, M., Nannapaneni, R., Kode, D., Chang, S., Sharma, C., McDaniel, C., and Kiess, A. 2019. Rugose morphotype in Salmonella typhimurium and S. heidelberg induced by sequential exposure to subinhibitory NaOCl aids in biofilm tolerance to lethal NaOCl on polystyrene and stainless steel surfaces. Frontiers in Microbiology 10:2704.

Abeysundara, P., Dhowlaghar, N., and Nannapaneni, R. 2019. Influence of cold stress on the survival of Listeria monocytogenes Bug600 and ScottA in lethal alkali, acid and oxidative stress. LWT - Food Science and Technology 100:40-47.

Dhowlaghar, N., Shen, Q., Nannapaneni, R., Schilling, M. W., and Samala, A. 2019. Survival of acid stress adapted cells of Listeria monocytogenes serotypes 1/2a and 4b in commonly used disinfectants in broth and water models. LWT Food Science and Technology 109:201-206.

Presentations
Kargar, M. (Presenter), F.M. Woods, M.M. Wall, E.G. Fonsah, J.R. Kessler, K. Shetty, R.B. Jeganathan, and N. Larsen. Influence of maturity on physiochemical quality of genomically diverse banana (Musa spp.) cultivars. 82nd Annual Southern Region American Society for Horticultural Science, Feb 2-4, 2018, Jacksonville, FL

Kargar, M. (Presenter), F.M. Woods, M.M. Wall, E.G. Fonsah, J.R. Kessler, K. Shetty, R.B. Jeganathan, and N. Larsen. Preliminary study of enzymatic browning susceptivility in banana peel and pulp tissues in relation to genotype and ripening. 115th Annual American Society for Horticultural Science, July 31-August 3, 2018, Washington, D.C.

Woods, F.M. (Presenter), A. Gebrekidan, E. Coneva, E. Vinson, K. Shetty, D. Sarkar, B.D. Blasius and A. Caylor. Influence of cultivar, environment and growing season on phytochemical properties of Alabama-grown Rabbiteye blueberries (Vaccinium ashei Reade). 4th NDSU Annual Conference on Food for Health, July 8 - 11, 2018, Fargo, North Dakota

Miranda, M. (Presenter), Marcos, D., Garrido Assis, O.B., Sun, X., Ference, C., Plotto, A., Bai, J., Baldwin, E. Effect of ginger essential oil on citrus fruit decay when applied in a nano-emulsion coating.

Becerra, C. (Presenter), Plotto, A., Du, X. Strawberry flavor reconstitution and the impact of methyl anthranilate on strawberry flavor. Institute of Food Technologists (IFT), Jun-19, New Orleans, LA

Bai, J. (Presenter), Sun, X., Baldwin, E.A., Plotto, A. Chlorine dioxide as a tool for food safety and reduction of postharvest decay. Specialty Crops Block Grant Showcase, 29-Oct-19, Gainesville, FL

Bai, J. (Presenter), Baldwin, E.A., Plotto, A., Driggers, R.E., Stover, E.W. Classification of USDA orange-mandarin hybrids using volatile profiles. American Chemical Society (ACS), 31-Mar-19 , Orlando, FL

Ali, S. (Presenter), Plotto, A., Scully, B.T., Stover, E.W., Pisani, C., Ritenour, M., Bai, J. Fatty acid composition and aroma volatile profiling of avocado germplasm from subtropical regions. American Chemical Society (ACS), 31-Mar-19, Orlando, FL

Marin, A. (Presenter), Baldwin, E.A., Bai. J., Wood, D.R., Brecht, J., Plotto, A. Edible coatings as carriers of antioxidant compounds to maintain appealing appearance of fresh cut mango. Florida State Horticultural Society, 10-Jun-19, Orlando, FL

Marin, A. (Presenter), Sun, X., Miranda, M., Ference, C.M., Bai, J., Ritenour, M., Plotto, A. Optimizing essential oil applications to prevent postharvest decay in strawberries. Florida State Horticultural Society, 10-Jun-19, Orlando, FL

Belisle, C.E. (Presenter), Kim, J., Sargent, S.A., Brecht, J.K., and Sandoya, G.V. "Reducing pink rib, a stress-induced disorder in lettuce. Plant Science Symposium 2018, Univ. of Florida, Gainesville, FL

Belisle, C.E. (Presenter), Kim, J., Sargent, S.A., Brecht, J.K., and Sandoya, G.V. Mitigating pink discoloration in lettuce. Everglades Research & Education Center Open House. [Poster], 2019, Belle Glade, FL

Belisle, C. (presenter), G.V. Sandoya, S.A. Sargent, and J. K. Brecht. Developing a methodology to screen Lactuca sativa cultivars and breeding lines to predict shelf life potential. Florida State Horticultural Society Annual Meeting, 2019, Orlando, FL

Bertoldi, B. (Presenter), J. De, C. Baker, C. Pabst, A. Gutierrez and K.R. Schneider. Determining water quality and bacterial load on tomatoes in flume tanks from Florida packinghouses. International Assoc. of Food Protection, 2019, Louisville, KY

Brecht, J.K. (Keynote Presenter). The physiological basis of fresh–cut fruit and vegetable differences. ISHS IV International Conference on Fresh-Cut Produce, 2019, Taian, China

Brecht, J.K. (Presenter). Fresh–cut quality issues regarding cell integrity, translucency & juice leakage. Fresh–Cut Products: Maintaining Quality and Safety Workshop, 2019, Univ. of Calif., Davis, CA

Brecht, J.K. (Presenter). Modified atmospheres: Benefits and risks to fresh–cut produce. Fresh–Cut Products: Maintaining Quality and Safety Workshop, 2019, Univ. of Calif., Davis, CA

Brecht, J.K. (Presenter). Transportation and distribution issues. Fresh–Cut Products: Maintaining Quality and Safety Workshop, 2019, Univ. of Calif., Davis, CA

De, J. (Presenter), Bertoldi B., Pabst, C.R., Baker C.A., Brecht J.K., Sargent S.A., and Schneider K.R. Comparing efficacy of hydrocooling with different concentrations of free chlorine in reducing microbial load from whole corn. Poster #P1-22. International Assoc. of Food Protection, 2019, Louisville, KY

De J. (Presenter), Bertoldi B., Pabst, C.R., Baker C.A., Gutierrez A., Sargent S.A., and Schneider K.R. Evaluation of hydrocooling with two different sanitizers in reducing microbial load from whole corn and their shelf life. Poster #P1-119. International Assoc. of Food Protection, 2019, Louisville, KY

Theodore, C. (Presenter), S.A. Sargent, J.K. Brecht, and L. Zotarelli. Cooling method affects postharvest quality of new breeding lines and commercial broccoli cultivars in Florida. Florida State Horticultural Society Annual Meeting, 2019, Orlando, FL

Theodore, C. (Presenter), S.A. Sargent, J.K. Brecht, and L. Zotarelli. Effect of cooling methods on postharvest quality of commercial broccoli cultivars grown in Florida. American Society for Horticultural Science, 2019, Las Vegas, NV

Xie, Y., J.K. Brecht (Presenter), C. Abrahan, Y. Luo, and E.R. Bornhorst. Minimizing quality deterioration of fresh–cut baby spinach by retrofitting refrigerated retail display cases with doors. (e-Poster). ISHS IV International Conference on Fresh-Cut Produce, 2019, Taian, China
Almenar, E. (Presenter). New Packaging materials for fresh-cut produce. IV International Conference on Fresh-cut Produce, August 14, 2019, Taian, Shandong, China

Spruit, D. (Presenter), Almenar, E., Peters, A., Salva, S. Project E-COMMFOOD. Phase 1:E-commerce food packaging market study. ISTA. The Omni-Channel Packaging Strategies Conference 2019, October 2, 2019, Chicago, IL

Fehlberg, J. (Presenter), Almenar, E. Use of orange juicing waste for development of food packaging film. IFU Juice Conference 2019, May 27-30, 2019, Playa Del Carmen, Mexico
Kode, D. (Presenter), Nannapaneni, R., Bansal, M., Cheng, W.-H., Sharma, C., Kiess, A. Homologous stress adaptation in four strains of Listeria monocytogenes to quaternary ammonium compounds after sublethal exposure. Annual Meeting of the International Association for Food Protection, 7/21/2019 to 7/24/2019, Louisville, KY

Kode, D. (Presenter), Nannapaneni, R., Bansal, M., Cheng, W.-H., Sharma, C., Kiess, A. Heterologous stress adaptation to gentamicin in four strains of Listeria monocytogenes after sublethal adaptive response to quaternary ammonium compound (QAC). Annual Meeting of the International Association for Food Protection, 7/21/2019 to 7/24/2019, Louisville, KY

Kode, D. (Presenter) and Nannapaneni, R. Stress adaptation in Listeria monocytogenes to quaternary ammonium compound (QAC) after sublethal exposure. Summer Student Science Symposium of the Mississippi Academy of Sciences, 7/11/19, Mississippi State University, MS