SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Pritsos, Cris (pritsos@cabnr.unr.edu) – University of Nevada Reno; Chicco, Adam (adam.chicco@colostate.edu) – Colorado State University; Williams, David (david.williams@oregonstate.edu) – Oregon State University; Turner, Nancy (ndturner@anr.msu.edu) – Michigan State University; Weir, Tiffany (tiffany.weir@colostate.edu) – Colorado State University; Delgado, Efren (edelgad@nmsu.edu) – New Mexico State University; Majumder, Kaustav (kasutav.majumder@unl.edu) – University of Nebraska Lincoln; Nerurkar, Pratibha (pratibha@hawaii.edu) – University of Hawaii; Chen, Chi (chichen@umn.edu) – University of Minnesota; Ilce Medina (ilce@msu.edu) – Michigan State University; Maria Marco (mmarco@ucdavis.edu)– University of California Davis; Jacques Izard (jizard@unl.edu)– University of Nebraska; Ock Chun (ock.chun@uconn.edu)– University of Connecticut ; Mohit Verma (msverma@purdue.edu)– Purdue University ; Nick Bello (ntbello@rutgers.edu)– Rutgers University; Satish Kumar Natarajan (snatarajan2@unl.edu) – University of Nebraska; Claudia Maier (claudia.maier@oregonstate.edu)– Oregon State University

W4122-Multistate-Group-2020-Annual-Meeting-Minutes.docx

Accomplishments

Below is a summary of research accomplishments of W4122 research groups representing 13 US states address the 4 primary objective of our multistate project:

Objective 1: Examine the effects of phytochemicals and other dietary components on gut microbiota and intestinal function.

W4122 researchers in California investigated how proteins and metabolites made by Lactiplantibacillus (Lactobacillus) plantarum contained in fresh, fermented fruits and vegetables and ingested as probiotics can stimulate intestinal epithelial cell repair. In other studies, they studied how L. plantarum can reduce its extracellular environment through extracellular electron transport pathways, which is important in the production of fermented foods and may be useful for modifying the intestinal environment to reduce oxidative stress.

W4122 researchers in California continued our investigations to elucidate how resistant starch type 2  (RS2) and other dietary fibers, including whole grains (WG), may reduce the risk for obesity and type 2 diabetes as a consequence of stimulating (and minimizing) growth of certain bacteria in the large intestine. These findings show that future studies may lead to the fine-tuning of dietary fiber composition so that it is more useful for targeting selected bacterial taxa in the intestine that may be needed to achieve a specific and intended health outcome. 

W4122 researchers in Nebraska worked in collaboration with Harvard T.H. Chan School of Public Health on statistical methodology to analyze microbiome data concomitantly to dietary data. They collected data from 51,529 men enrolled in the Health Professionals Follow-up Study since 1986 to determine the association between sulfur-metabolizing bacteria in stool and risk of colorectal cancer (CRC) over 26 years of follow-up. Results show that foods associated with higher sulfur microbial diet scores (processed meats and low-calorie drinks and lower consumption of vegetables and legumes) were associated with greater risk of distal colon and rectal cancers, after adjusting for other risk factors.  Further studies are needed to determine how sulfur-metabolizing bacteria might contribute to CRC pathogenesis.

W4122 researchers in Indiana investigated a series of twelve carbohydrates (monosaccharides and disaccharides) that are building blocks of human milk oligosaccharides and used them as a carbon source for the growth of Escherichia coli LF82 and Bacteroides fragilis from the human gut microbiome. After obtaining data on the growth of these bacteria in anaerobic conditions, we observed deterministic behavior based on the type of carbon source used, and used a modified logistic model to characterize these growth profiles. Results indicate that bacteria can experience a death phase which is important in determining microbe-microbe interactions.

W4122 researchers in Minnesota investigated the biotransformation and metabolic fates of both nutrients and non-nutrients (phytochemicals and microbial metabolites) in the GI tract through LC-MS-based metabolomics analysis. In particular, LC-MS-based untargeted profiling was performed on the lipid extracts from wild rice, white rice, and brown rice, and the phytosterol content of wild rice was further analyzed through the quantification of γ-oryzanol and stigmasterol, illustrating the enrichment of these phytosterols in wild rice due to its unique processing after harvesting.

W4122 researchers in Colorado completed several human clinical trials focused on the interaction between dietary supplements and gut health, specifically examining the effects of a Phage-based "prebiotic" in combination with a Bifidobacterium probiotic, a Bifidobacterium probiotic alone, and a novel Bacillus subtilis probiotoc on gut health, immune parameters, inflammation, and gut microbiota composition. Primary findings include 1) evidence that Bacillus subtilis DE111 improved lipid parameters, endothelial function, and modulated immune cell populations both in the basal state and in response to pro-inflammatory stimuli.2) A phage cocktail, PreforPro, when administered with a probiotic, extended benefits to gut health over consumption of the probiotic alone and favorably altered the gut microbiota toward a more anti-inflammatory state, and 3) Bifidobacterium lactis consumption improved several self-assessed parameters of intestinal health over placebo ingestion.

W4122 researchers in Colorado in collaboration with Dr. Henry Thompson, established that dry bean and pulse consumption offsets the negative metabolic effects of a high fat diet in diet-induced obesity in rodent models, which may be driven increases in Akermansia muciniphila. This mucin-consuming gut microbial species has been shown to improve metabolic parameters in clinical trials with obese humans as well. Finally, these studies have revealed that there are differences between types of legumes in terms of their impacts on the microbiota and metabolic health. Further research is being done to establish the mechanistic basis of these differences.

W4122 researchers in Oregon continued their investigation of Xanthohumol (XN), a prenylated flavonoid found in the flowers of hops, which they have shown to have anti-inflammatory properties, acts as an antioxidants, have chemopreventive properties and improve metabolic syndrome in rodent models. Their new works found that XN modulates the gut microbiome composition, modulates the fecal metabolome including bile acid profiles, and reduces adipose inflammation, which early evidence for effects on liver and brain lipid changes suggestive of effects on neurocognitive performance. 

W4122 researchers in Oregon developed chemical fingerprinting methods for the characterization of botanical extracts of Centella asiatica (CA), an edible plant associated with cognitive benefits in pre-clinical models,  to ensure product integrity of extracts. These studies identified caffeoylquinic acids as contributors of bioactivity an confirmed minimal induction or inhibition of a range of P450 enzymes, including CYP3A4, by the CA extract, suggesting a low potential for drug interactions modulated by P450 metabolism.

 W4122 researchers in Michigan have demonstrated that diets containing certain polyphenolic compounds (derived from dried plums) impact the microbiota and their metabolism, which has significant impacts on multiple pathways involved in inflammation and tumorigenesis involved in the development of colorectal cancers. 

W4122 researchers in New Jersey examined the metabolic effects of raspberry ketone (RK)on preventing diet-induced obesity, specifically examining the absorption, metabolism, and distribution of acute orally administered RK  in mice. Results indicate that RK is highly bioavailable, rapidly metabolized, and exhibits significantly different pharmacokinetic behaviors between obese and control mice. In other experiments, they determined that RK has dose-differential effects on feeding patterns and cardiovascular function, which needs consideration as it is used as a nutraceutical for weight control for obesity. In particular, results indicated a dose-dependent feeding suppression with acute RK, but doses that reliable suppress food intake are associated with pathological changes.

Objective 2: Identify cellular mechanisms and host molecular targets of beneficial or adverse dietary components that influence human health.

and

Objective 3: Explore the interaction between dietary components and the host metabolome and epigenome.

W4122 researchers in Connecticut examined the relationship between citrus consumption and cutaneous melanoma risk among 56,205 Caucasian postmenopausal women in the Women’s Health Initiative. Cox proportional hazards models were used to estimate hazard ratios (HRs) and 95% confidence intervals (CIs) of melanoma by citrus intake level. During a mean follow-up of 15.7 years, 956 incident melanoma cases were documented. In multivariable adjusted models, the HR (95% CI) for melanoma was 1.12 (0.91, 1.37) among the highest citrus consumers (1.5+ servings/day of fruit or juice) versus the lowest (<2 servings/week), 0.95 (0.76, 1.20) among the highest citrus fruit consumers (5+ servings/week) versus non-consumers, and was 1.13 (0.96, 1.32) for the highest citrus juice consumers (1+ servings/day) versus the lowest (<1 serving/week). In stratified analyses, an increased melanoma risk associated with citrus juice intake was observed among women who spent the most time outdoors in summer as adults; the HR for the highest versus lowest intake was 1.22 (1.02, 1.46) (p trend = 0.03).

W4122 researchers in Colorado completed two pilot clinical studies exploring the pharmacokinetics of different cannabidiol preparations and the individual factors (such as body composition) that may influence the absorption and clearance of these compounds in humans. Specifically, we found that water soluble preparations, when consumed as a beverage, had increased bioavailability over lipid soluble preparations, and that fat free mass was a significant predictor of time to peak concentration for the preparation that had the greatest Cmax.

W4122 researchers in Nebraska evaluated the benefits of dietary γ-glutamyl peptides in the prevention of vascular disorders. Based on evidence that food-derived dietary bioactive peptides exhibit antioxidant and anti-inflammatory properties, and the well-established role of chronic inflammation in obesity-associated vascular diseases, studies were performed to test the biological activity of dietary γ-glutamyl peptide (γ-EV) in vascular endothelial cells (HAoEC: Human Aortic Endothelial Cells). Results indicate that γ-EV reduce inflammation in by activating the calcium sensing-receptor (CaSR), and can be absorbed via Pep-T1 receptor.

W4122 researchers in Nebraska have developed a green chemistry based scalable extraction method to isolate γ-glutamyl peptides from Nebraskan Great Northern Beans.

W4122 researchers in Nebraska evaluated the effects of omega-3 Fatty Acid-Derived Resolvin D2 on placental trophoblast activities, which is important for pregnancy and healthy neonatal development. One mechanism by which omega-3 fatty acids exert their protective effects is through serving as substrates for the generation of specialized pro-resolving lipid mediators (SPM) that potently limit and resolve inflammatory processes. Researchers identified GPR18, an omega-3 fatty acid receptor, in placental vascular smooth muscle and extravillous trophoblasts, and determined that its stimulation with RvD2 led to significantly altered responsiveness to inflammatory stimulation in human umbilical artery smooth muscle cells and placental trophoblasts. Taken together, these findings establish a role for SPM actions in human placental tissue.

W4122 researchers in Hawaii are studying the effect of Hawaii-grown coffee on plasma adipocytokine levels among healthy individuals.

W4122 researchers in Tennessee, in collaboration with the vascular surgeons of the University of Tennessee Medical Center, started a clinical trial investigating the impact of a combination of dietary bioactives in the stability of peripheral stents. This work was based on a preclinical model of vascular injury (Buckley et al. Nutr Res 68:34-44.) However, the Clinical Trial has been suspended since March 1, 2020, due to Covid-19.

W4122 researchers in Minnesota are developing methods to discover unreported metabolic events in diverse biological samples, including urine, blood, other biofluids, and tissue samples through LC-MS-based metabolomic analysis. In a recent study, six thermally oxidized soybean oils (OSO) with a wide range of quality measures were prepared under different processing temperatures and processing durations. Broilers and nursery pigs were randomly assigned to diets containing either unheated control soybean oil or one of six OSO. Animal performance was determined by measuring body weight gain, feed intake, and gain to feed ratio, and correlated with chemical profiles of OSOs, revealing several associations with growth performance in both broilers and pigs.  

W4122 researchers in Oregon, in collaboration with Lawrence Livermore National Laboratory and Pacific Northwest National Laboratory, analyzed benzo[a]pyrene (a polycyclic aromatic hydrocarbon, (PAH)) in humans following oral exposure in an ongoing effort to determine the impact of food containing high levels of multiple PAHs on human health.

W4122 researchers in Colorado are investigating how maternal high-fat feeding and omega-3 fatty acid supplementation alters fetal metabolism in a manner that may influence the susceptibility of offspring to developing obesity or diabetes in late childhood.  Teams are using an ovine model to investigate the role of placental fatty acid transport in mediating this response, perhaps leading to novel therapies or dietary interventions that can protect the fetus from a potentially harmful environment during development.

Objective 4: Determine how food processing influences chemical composition to affect human health.

W4122 researchers in New Mexico are evaluating the use of hydrolyzed food waste as an economical and sustainable alternative to be employed as a culture medium and add value to Agroindustrial byproducts. The proposed technology is also useful on obtaining specific heterologous proteins, single-cell proteins and single-cell oils derived from the yeast.  In this work, hydrolyzed agro-industrial wastes from chili, sugarcane bagasse and GCSM are used as liquid culture mediums to grow the modified yeast Yarrowia lipolytica to express oleosin from glandless cottonseed protein.

W4122 researchers in Hawaii used metabolomics and lipidomics approach to identify environmental effects not only on polyphenols but also signatures of secondary metabolites of cacao, a polyphenol are associated with several health benefits including improving metabolic disorders.

Impacts

  1. W4122 researchers in California worked to understand and improve microbe-based therapies and dietary measures to optimize human health. Particular efforts focus on characterizing specific bacteria taxa and metabolites consumed in foods and beverages that act through the intestinal epithelium to reduce the prevalence of obesity and type 2 diabetes.
  2. W4122 researchers in Oregon made inroads in studying the effects of Centella asiatica extracts on enhancing neurological and functional resilience in pre-clinical models by developing a MS-based chemical fingerprinting method for CA extracts that is also broadly applicable to other botanical extract, and showing that these extracts have low potential for P450 drug interactions (an analysis required by the FDA to move forward with clinical trials).
  3. W4122 researchers in Oregon found that hops‐derived xanthohumol (XN) and its derivatives reduce high‐fat diet (HFD)‐induced obesity and metabolic syndrome in part by modulate the gut microbiota, altering bile acid metabolism and lower adipose inflammation (in rodents). They also found that these compounds decrease ceramide content in liver and hippocampus, which may serve to reverse obesity-associated neurocognitive decline.
  4. W4122 researchers in Nebraska completed enrollment in recruitment database to facilitate phenotype-based population selection, and completed sample collection via a pandemic resilient process.
  5. W4122 researchers in Indiana discovered that the growth profiles of bacteria depend on the type of carbohydrate fuel that supports them. By modeling the death phase in the quantification of this process, they can better predict microbe-microbe interactions and thus assembly and disruption of the gut microbiota for improving human health outcomes.
  6. W4122 researchers in Nebraska provided the first demonstration that food consumption contributing to high sulfur microbial diet scores, including increased intake of processed meats and low-calorie drinks and fewer vegetables and legumes, are associated with development of distal CRC. This transdisciplinary work combined microbiome study, nutritional epidemiology, cancer research, traditional and high-dimensional statistics (big data), and biocomputing.
  7. W4122 researchers in Nebraska included the goals and fundamental scientific bases of the project to the University of Nebraska at Lincoln course targeting graduate and undergraduate students with the title “Omnivore’s Digestive-Tract Microbiome”.
  8. W4122 researchers in Nebraska developed 3D printed representative members of the human microbiome as an educative tool for K12 and college level courses. Many teaching objectives can be achieved including understand spatial relationships in biofilms, shape parameters in bacteriology, description of commensals and pathogens.
  9. W4122 researchers in Nebraska identified that omega-3 fatty acids are metabolized into bioactive pro-inflammation resolution lipids that confer protection against inflammation in the placenta, perhaps through the novel discovery of GPR18, a RvD2 receptor in placental trophoblasts, suggesting that omega-3 fatty acid metabolites have a functional role in the placenta.
  10. W4122 researchers in New Mexico determined that the expression of oleosin from glandless cottonseed meal protein has the potential to be used as an emupsifying protein in food products and aquaculture feed.
  11. W4122 researchers in Connecticut provided epidemiological evidence of the risk of furocoumarins on skin health to the public health arena, which will help the public understand the effects of citrus fruits and furocoumarin-rich foods on skin cancer risk. In particular, results suggested that higher consumption of citrus juices may increase risk of melanoma among women spending at least 30 minutes outdoors daily during the summer as adults.
  12. W4122 researchers in Nebraska made progress toward developing clinically applicable dietary-peptide-based therapeutics for the improved treatment of atherosclerosis and associated cardiovascular diseases.
  13. W4122 researchers in Minnesota demonstrated that chemometrics and metabolomics are effective analytical approaches for defining the chemical composition and metabolic fates of feed ingredients. Unique chemical components in foods were identified, and the associations of feed ingredients and growth performance were established in our studies. These results could be used to understand the metabolic consequences of dietary bioactives.
  14. W4122 researchers in Michigan demonstrated that diets containing certain polyphenolic compounds (derived from dried plums) impact the microbiota and their metabolism, which has significant impacts on multiple pathways involved in inflammation and tumorigenesis. Importantly, systemic benefits are also derived from consuming these polyphenol-rich fiber sources and their microbial metabolites.
  15. W4122 researchers in Colorado are establishing mechanistic links between gut microbiota and intestinal function and early predictors of cardiovascular disease risk that may lead to novel therapeutic approaches for heart disease prevention.
  16. W4122 researchers in Colorado have identified metabolomic signatures associated with high and low fruit and vegetable intake that may be more precise in predicting intake of these foods than the currently used self-report methods.
  17. W4122 researchers in Colorado completed two pilot clinical studies exploring the pharmacokinetics of different cannabidiol preparations and found that water soluble preparations, when consumed as a beverage, had increased bioavailability over lipid soluble preparations, and that fat free mass was a significant predictor of time to peak concentration for the preparation that had the greatest Cmax.
  18. W4122 researchers in Colorado completed several human clinical trials that linked intake of probiotic supplements to improvements in gut health, immune parameters, inflammation, and vascular function in humans.
  19. W4122 researchers in Colorado established that dry bean and pulse consumption offsets the negative metabolic effects of a high fat diet in diet-induced obesity in rodent models, which may be driven increases in a particular mucin-consuming gut microbial species has been shown to improve metabolic parameters in clinical trials with obese humans. These studies revealed that there are differences between types of legumes in terms of their impacts on the microbiota and metabolic health. Further research is being done to establish the mechanistic basis of these differences.
  20. W4122 researchers in Colorado have discovered that maternal high-fat feeding alters fetal metabolism in a manner that might predispose offspring to the development of diabetes in late childhood. Teams are using an ovine model to investigate the role of placental fatty acid transport in mediating this response, perhaps leading to novel therapies or dietary interventions that can protect the fetus from a potentially harmful environment during development.
  21. W4122 researchers in New Jersey discovered that a component of red raspberries (raspberry ketone) has anti-obesity effects in mice, perhaps in part through metabolites of gut microbes and their effect on adipose tissue biology and feeding behavior.
  22. W4122 researchers in Oregon, in collaboration with Lawrence Livermore National Laboratory and Pacific Northwest National Laboratory, performed the first pharmacokinetic analysis of benzo[a]pyrene (a polycyclic aromatic hydrocarbon, (PAH)) in humans following oral exposure and the impact of food containing high levels of multiple PAHs.
  23. W4122 researchers in Illinois have shown that there is a clear effect of thermally-abused frying oil (TAFO) on progression of 4T1 cells injected into the tibia of mice, indicating links between consumption of fried foods using these oils and the development of cancer.

Publications

There were 71 new publications by W4122 members in 2019-2020 period, addressing the effects of bioactive nutrients on health and chronic disease risk, basic insights into nutrient metabolism, and the development of new methodology and technologies for studying these processes in humans and biological model systems.  Publcations are listed below with W4122 group members in boldfaced text.

Riley L. Hughes, Mary E. Kable, Maria L. Marco, Nancy L. Keim, 2019. The role of the gut microbiome in predicting response to diet and the development of precision nutrition models. Part II: Results. Advances in Nutrition. Nov 1;10(6):979-998.

Solomon A. Mekonnen, Daniel Merenstein, Claire M. Fraser, and Maria L. Marco. 2020. Molecular mechanisms of probiotic prevention of antibiotic-associated diarrhea. Current Opinion in Biotechnology. 61:226-234

Zachary A. Bendiks, Knud E.B. Knudsen, Michael J. Keenan, and Maria L. Marco. 2020. Conserved and variable responses of the gut microbiome to resistant starch type 2. Nutrition Research. 77:12-28. doi: 10.1016/j.nutres.2020.02.009

Derrick Risner, Maria L. Marco, Sara A. Pace, and Edward S. Spang. 2020. The potential production of the bioactive compound pinene using whey permeate. Processes. 8(3), 263

Paraiso IL, Revel JS, Choi J, Miranda CL, Lak P, Kioussi C, Bobe G, Gombart AF, Raber J, Maier CS, Stevens JF. Targeting the Liver-Brain Axis with Hop-Derived Flavonoids Improves Lipid Metabolism and Cognitive Performance in Mice. Mol Nutr Food Res. 2020 Aug;64(15):e2000341. doi: 10.1002/mnfr.202000341. Epub 2020 Jul 6. PMID: 32627931.

Logan IE, Bobe G, Miranda CL, Vasquez-Perez S, Choi J, Lowry MB, Sharpton TJ, Morgun A, Maier CS, Stevens JF, Shulzhenko N, Gombart AF. Germ-Free Swiss Webster Mice on a High-Fat Diet Develop Obesity, Hyperglycemia, and Dyslipidemia. Microorganisms. 2020 Apr 5;8(4):520. doi:10.3390/microorganisms8040520. PMID: 32260528; PMCID: PMC7232377.

Zhang Y, Bobe G, Revel JS, Rodrigues RR, Sharpton TJ, Fantacone ML, Raslan K, Miranda CL, Lowry MB, Blakemore PR, Morgun A, Shulzhenko N, Maier CS, Stevens JF, Gombart AF. Improvements in Metabolic Syndrome by Xanthohumol Derivatives Are Linked to Altered Gut Microbiota and Bile Acid Metabolism. Mol Nutr Food Res. 2020 Jan;64(1):e1900789. doi: 10.1002/mnfr.201900789. Epub 2019 Dec 15. PMID: 31755244; PMCID: PMC7029812.

Matthews DG, Caruso M, Alcazar Magana A, Wright KM, Maier CS, Stevens JF, Gray NE, Quinn JF, Soumyanath A. Caffeoylquinic Acids in Centella Asiatica Reverse Cognitive Deficits in Male 5XFAD Alzheimer's Disease Model Mice. Nutrients. 2020 Nov 13;12(11):3488. doi: 10.3390/nu12113488. PMID: 33202902; PMCID: PMC7698091.

Wright KM, Magana AA, Laethem RM, Moseley CL, Banks TT, Maier CS, Stevens JF, Quinn JF, Soumyanath A. Centella asiatica Water Extract Shows Low Potential for Cytochrome P450-Mediated Drug Interactions. Drug Metab Dispos. 2020 Oct;48(10):1053-1063. doi: 10.1124/dmd.120.090860. Epub 2020 Jun 24. PMID: 32581050; PMCID: PMC7543484.

Alcazar Magana A, Wright K, Vaswani A, Caruso M, Reed RL, Bailey CF, Nguyen T, Gray NE, Soumyanath A, Quinn J, Stevens JF, Maier CS. Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban. Phytochem Anal. 2020 Nov;31(6):722-738. doi: 10.1002/pca.2936. Epub 2020 Apr 12. PMID: 32281154; PMCID: PMC7587007.

Selamassakul O, Laohakunjit N, Kerdchoechuen O, Yang L, Maier CS. Bioactive  peptides from brown rice protein hydrolyzed by bromelain: Relationship between biofunctional activities and flavor characteristics. J Food Sci. 2020 Mar;85(3):707-717. doi: 10.1111/1750-3841.15052. Epub 2020 Feb 11. PMID: 32043604.

Song Z, Pearce MC, Jiang Y, Yang L, Goodall C, Miranda CL, Milovancev M, Bracha S, Kolluri SK, Maier CS. Delineation of hypoxia-induced proteome shifts in osteosarcoma cells with different metastatic propensities. Sci Rep. 2020 Jan 20;10(1):727. doi: 10.1038/s41598-019-56878-x. PMID: 31959767; PMCID: PMC6971036.

Preston GW, Yang L, Phillips DH, Maier CS. Visualisation tools for dependent peptide searches to support the exploration of in vitro protein modifications. PLoS One. 2020 Jul 8;15(7):e0235263. doi: 10.1371/journal.pone.0235263. PMID: 32639981; PMCID: PMC7343161.

Magana AA, Reed RL, Koluda R, Miranda CL, Maier CS, Stevens JF. Vitamin C Activates the Folate-Mediated One-Carbon Cycle in C2C12 Myoblasts. Antioxidants (Basel). 2020 Mar 5;9(3):217. doi: 10.3390/antiox9030217. PMID: 32150984; PMCID: PMC7139526.

Maruthamuthu, M.K., Raffiee, A.H., De Oliveira, D.M., Ardekani, A.M.,, Verma, M.S. (2020) Raman spectra-based deep learning – a tool to identify microbial contamination MicrobiologyOpen (in press).

Yildirim, A., Grant, J.C., Siamak, S.E., Lee, W., Maruthamuthu, M.K., Verma, M., Sutherland, J.W., Cakmak, M. (2020) Roll‐to‐Roll (R2R) Production of Large‐Area High‐Performance Piezoelectric Films Based on Vertically Aligned Nanocolumn Forests Advanced Materials Technologies. DOI: 10.1002/admt.202000553.

Maruthamuthu, M.K., Rudge, S.R., Ardekani, A.M., Ladisch, M.R., Verma, M.S. (2020) Process Analytical Technologies and Data Analytics for Manufacture of Monoclonal Antibodies Trends in Biotechnology. DOI: 10.1016/j.tibtech.2020.07.004.

Waimin, J.F.,§ Nejati, S.,§ Jiang, H., Qiu, J., Wang, J., Verma, M.S., Rahimi, R. (2020) Smart Capsule for Non-invasive Sampling and Studying of the Gastrointestinal Microbiome RSC Advances; 10: 16313-16322. DOI: 10.1039/C9RA10986B

Zhao D, Yuan B, Kshatriya D, Polyak A, Simon JE, Bello NT, Wu Q. Influence of Diet-Induced Obesity on the Bioavailability and Metabolism of Raspberry Ketone (4-(4-Hydroxyphenyl)-2-Butanone) in Mice. Mol Nutr Food Res. 2020 Apr;64(8):e1900907. doi: 10.1002/mnfr.201900907. Epub 2020 Feb 25. PMID: 32052560; PMCID: PMC7329366.

Yuan B, Zhao D, Kshatriya D, Bello NT, Simon JE, Wu Q. UHPLC-QqQ-MS/MS method development and validation with statistical analysis: Determination of raspberry ketone metabolites in mice plasma and brain. J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jul 15;1149:122146. doi: 10.1016/j.jchromb.2020.122146. Epub 2020 May 19. PMID: 32474352; PMCID: PMC7391222.

Kshatriya D, Hao L, Li X, Bello NT. Raspberry Ketone [4-(4-Hydroxyphenyl)-2-Butanone] Differentially Effects Meal Patterns and Cardiovascular Parameters in Mice. Nutrients. 2020 Jun 11;12(6):1754. doi: 10.3390/nu12061754. PMID: 32545402; PMCID: PMC7353175.

Hao L, Kshatriya D, Li X, Badrinath A, Szmacinski Z, Goedken MJ, Polunas M, Bello NT. Acute feeding suppression and toxicity of raspberry ketone [4-(4-hydroxyphenyl)-2-butanone] in mice. Food Chem Toxicol. 2020 Sep;143:111512. doi: 10.1016/j.fct.2020.111512. Epub 2020 Jun 19. PMID: 32565406; PMCID: PMC7483346.

Wang Y, Miller JW, Bello NT, Shapses SA. Low-vitamin-D diet lowers cerebral serotonin concentration in mature female mice. Nutrition Research. 2020 Sep;81:71-80. doi: 10.1016/j.nutres.2020.07.006. Epub 2020 Jul 24. PMID: 32920521.

Muthuraj PG, Natarajan, SK. Fetal Programming in Maternal Obesity. Diabesity 2020; 6(3): 36-39. DOI:10.15562/diabesity.2020.71 Review

Thoene M, Van Ormer M, Lyden E, Thompson M, Yuil-Valdes A, Natarajan SK, Mukherjee M, Nordgren TM, Furtado, Anderson-Berry A, Hanson C, Snowden J. Inflammatory Compounds and Fat-Soluble Nutrients in Mother-Infant Dyads at Time of Delivery. Pediatr Res (2020). In Press

Liu B, Lu Y, Chen X, Muthuraj PG, Li X, Pattabiraman M, Zempleni J, Kachman SD, Natarajan SK, Yu J. Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. Nutrients. 2020 Feb 13;12(2):477. doi: 10.3390/nu12020477. PMID: 32069862.

Thoene M, VanOrmer M, Yuil-Valdes AG, Bruett T, Natarajan SK, Mukherjee M, Thompson M, Nordgren TM, Lippevelde WV, Overby, NC, Adu-Bonsaffoh K, Anderson-Berry A, Hanson C. Fat-Soluble Nutrients and Omega-3 Fatty Acids as Modifiable Factors Influencing Preterm Birth Risk. Placenta. 2020 Sep 1; 98:38-42. doi: 10.1016/j.placenta.2019.12.002. PMID: 33039030.

Ulu A, Sahoo PK, Yuil-Valdes AG, Mukherjee M, VanOrmer M, Muthuraj PG, Thompson M, Anderson-Berry A, Hanson CK, Natarajan SK*, Nordgren TM*. Omega-3 fatty acid-derived resolving D2 regulates human placental vascular smooth muscle and extravillous trophoblast activities. Int. J. Mol. Sci. 2019 Sep 7;20(18):4402. doi: 10.3390/ijms20184402. PMID: 31500240. *Co-corresponding author.

Johnson SA, Prenni J, Heuberger AL, Isweiri H, Chaparro J, Newman S, Uchanski M, Omerigic H, Michell K, Bunning M, Foster MT, Thompson HJ, Weir TL. Comprehensive Evaluation of Metabolites and Minerals in Six Microgreen Species and the Influence of Maturity. Current Development in Nutrition (in press)

Weir TL, McGinley JN, Neil ES, Thompson HJ (2020) Effect of pulse consumption on obesity and the metagenome, Proceedings of the 1st International Conference on Nutrients- Nutrition and Microbiota Effects on Chronic Disease. doi:10.3390/IECN2020-07009

Soderborg TK, Carpenter C, Janssen RC, Weir TL, Young BE, Krebs NF, Hernandez TL, Barbour LA, Frank DN, Kroehl, M, Friedman JE. (2020) Gestational diabetes is uniquely associated with altered early seeding of the infant gut microbiota. Front Endocrinol doi:10.3389/fendo.2020.603021

Grubb DS, Wrigley SD, Freedman KE, Wei Y, Vazquez AR, Trotter RE, Wallace TC, Johnson SA and Weir TL (2020) PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota. Nutrients 12, 2474; doi:10.3390/nu12082474

Weir TL, Trikha SRJ, Thompson HJ (2020) Diet and Risk of Cancer Reduction: The Role of Diet-Microbiota Interactions and Microbial Metabolites. Seminars in Cancer Biology DOI: 10.1016/ j.semcancer.2020.06.007

Trotter RE, Vazquez AR, Grubb D, Freedman KE, Wei Y, Jones S, Gentile CL, Melby CL, Johnson SA, Weir TL (2020) Examining the Impact of Probiotic Supplement Intake on Endothelial Function and Lipid Metabolism in Healthy Adults. Beneficial Microbes 11 (7): 621-630.

Lee DM, Ecton K, Trikha SR, Wrigley SD, Thomas K, Battson M, Wei Y, Johnson SA, Weir TL, and Gentile CL. (2020) Microbial Metabolite Indole-3-Propionic Acid Supplementation Does Not Protect Mice from the Cardiometabolic Consequences of a Western Diet. Am J Physiol-Gastro Liver Physiol doi.org/10.1152/ajpgi.00375.2019

Hobbs JM, Vazquez AR, Remijan ND, Trotter RE, McMillan TV, Freedman K, Wei Y, Arnold OR, Wolfe LM, Johnson SA, and Weir TL (2020) Evaluation of Pharmacokinetics and Acute Anti-Inflammatory Potential of Two Oral Cannabidiol (CBD)Preparations. Phytotherapy Research DOI:10.1002/ptr.6651

Michell KA*, Isweiri H, Newman SE, Bunning M, Bellows LM, Dinges ME*, Grabos LE*, Rao S, Foster MT, Heuberger AL, Prenni JE, Thompson HJ, Uchanski ME, Weir TL, Johnson SA (2020) Microgreens for the Masses: Exploring Consumer Acceptance and Sensory Perceptions of an Emerging Functional Food Crop. Journal of Food Science

McGinley JN, Fitzgerald VK, Neil ES, Omerigic HM, Heuberger AL, Weir TL, McGee R, Vandermark G, Thompson HJ. (2020) Pulse Crop Effects on the Gut Microbial Populations, Intestinal Function, and Adiposity in a Mouse Model of Dietary Induced Obesity. Nutrients 12:593; doi: 10.3390/nu12030593

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Puckett, D.L., Chahed, S., Alani, D.S., Donohoe, D.R., Whelan, J., and Bettaieb, A. 2020.  Zyflamend Induces Apoptosis in Pancreatic Cancer Cells via Modulation of the JNK Pathway. Cell Commun Signal. 18:126. (doi:10.1186/s12964-020-00609-7).

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Hung YT, Hu, Q, Faris RJ, Guo J, Urriola PE, Shurson GC, Chen C, Saqui-Salces M. Analysis of gastrointestinal responses revealed both shared and specific targets of zinc oxide and carbadox in weaned pigs. Antibiotics  9: 463 (2020)

Yuan J, Kerr BJ, Curry SM, Chen C, Identification of C9-C11 unsaturated aldehydes as prediction markers of growth and feed intake for non-ruminant animals fed oxidized soybean oil. J Anim Sci Biotechnol. 11: 49 (2020)

Chen D, Wiertzema JR, Peng P, Cheng Y, Wang Y, Liu J, Ma Y, Mosher W, Kang M, Min M, Chen P, Baumler DJ, Chen C, Lee L, Vickers Z, Feirtag J, Ruan R. Catalytic intense pulse light inactivation of Cronobacter sakazakii and other pathogens in non-fat dry milk and wheat flour. Food Chem. 332:127420 (2020)

Fritsch J, Garces L, Quintero MA, Pignac-Kobinger J, Santander AM, Fernández I, Ban YJ, Kwon D, Phillips MC, Knight K, Mao Q, Santaolalla R, Chen XS, Maruthamuthu M, Solis N, Damas OM, Kerman DH, Deshpande AR, Lewis JE, Chen C, Abreu MT, Low-fat, High-fiber Diet Reduces Markers of Inflammation and Dysbiosis and Improves Quality of Life in Patients With Ulcerative Colitis. Clin Gastroenterol Hepatol. 20: S1542-3565 (2020)

Sathe AG, Tuite P, Chen C, Ma Y, Chen W, Cloyd J, Low WC, Steer CJ, Lee BY, Zhu XH, Coles LD, Pharmacokinetics, Safety, and Tolerability of Orally Administered Ursodeoxycholic Acid in Patients With Parkinson's Disease—A Pilot Study, J Clin Pharmacol. 60: 744-750 (2020)

Cui W, Sathyanarayan A, Lopresti M, Aghajan M, Chen C, Mashek DG. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis. Autophagy. 19: 1-16 (2020)

An J, Liu J, Liang Y, Ma Y, Chen C, Cheng Y, Peng P, Zhou N, Zhang R, Addy M, Chen P, Liu Y, Huang G, Ren D, Ruan R. Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells. Food Chem. 322:126742 (2020)

Nerurkar PV*, Orias D, Soares N, Kumar M, Nerurkar VR. (2019) Momordica charantia (bitter melon) modulates adipose tissue inflammasome gene expression and adipose-gut inflammatory crosstalk in high-fat diet (HFD)-fed mice. J Nutr Biochem. 2019 Jun;68:16-32. doi: 10.1016/j.jnutbio.2019.03.003. Epub 2019 Mar 23. PMID: 31005847

Kar, A., Wei, X., Majumder, K., Eskridge, K., Handa, A., Subbiah, J. (2020). Effect of traditional and radiofrequency assisted thermal processing on the gel firmness of egg white powder. LWT-Food Science and Technology, 133: 110091.

Guha, S., Paul, C., Alvarez, S., Mine., Y., Majumder, K*. (2020). Dietary γ-glutamyl valine (γ-EV) ameliorates TNF-α induced vascular inflammation via endothelial calcium-sensing receptor (CaSR). Journal of Agricultural and Food Chemistry, 68: 9139.

Mine, Y., Majumder, K., Jin, Y., Zheng, Y. (2020). Chinese Sweet Tea (Rubus suavissimus) Polyphenols Attenuate the Allergic Responses in a BALB/C Mouse Model of Egg Allergy. Journal of Functional Foods, 67: 103827.

Kanouchi, K., Majumder, K., Shibata, H., Mine, Y. (2020). Lactobacillus pentosus S-PT84 and Rubus suavissimus leaf extract suppress lipopolysaccharide-induced gut permeability and egg allergen uptake. Food Production, Processing and Nutrition, 2:4.

Majumder, K., Jin, Y., Shibata, H., Mine, Y. (2020). Oral Intervention of Lactobacillus pentosus S-PT84 Attenuates the Allergenic Responses in a BALB/C Mouse Model of Egg Allergy. Molecular Immunology, 120: 43-51.

Jacques Izard, Teklu Kuru Gerbaba, and Shara R.P. Yumul. Three-dimensional printing of human microbiome constituents to understand spatial relationships and shape parameters in bacteriology, The American Biology Teacher - In press

Shajan Peter, Amanda Pendergraft, William VanDerPol, C. Mel Wilcox, Kondal R Kyanam Kabir Baig, Casey Morrow, Jacques Izard, Peter J. Mannon. Mucosa-associated microbiota in Barrett’s esophagus, dysplasia and esophageal adenocarcinoma differ similarly compared with healthy controls. Clinical and Translational Gastroenterology (2020) vol. 11(8) p e00199

Long H. Nguyen, Wenjie Ma, Dong D. Wang , Yin Cao, Himel Mallick, Teklu Gerbaba, Jason Lloyd-Price, Galeb Abu-Ali, A. Brantley Hall , Daniel Sikavi, David A.Drew, Raaj S. Mehta, Cesar Arze, Amit D. Joshi, Yan Yan, Tobyn Branck, Casey DuLong, Kerry Ivey, Shuji Ogino, Eric B. Rimm EB, Mingyang Song, Wendy S. Garrett, Jacques Izard, Curtis Huttenhower*, Andrew T. Chan. Association between sulfur-metabolizing bacterial communities in stool and risk of distal colorectal cancer in men. Gastroenterology (2020) vol. 158 (5) pp. 1313-1325 [Journal cover]

Rojas-Barboza D*, Park E,* Sassenfeld R, Winder J, Smith GB, Valles-Rosales D,  Delgado E,  Park YH. 2020. Rapid, Simple, Low-Cost Fluorescence Detection of Escherichia coli Using 3D-Printed Smartphone-Based Device. International Journal of Agricultural and Biological Engineering. Accepted for publication August 2020.

Matthews, A.*, Ulery, A., Rogus, S., Phillips, G., Delgado E. 2020. Heavy metal content of produce grown in San Juan County (New Mexico, USA). Journal of Environmental Science and Health, Part B. Published online. https://doi.org/10.1080/03601234.2020.1794220.

Delgado E, Gamero-Barraza G*, Flores-Rosas W, Valles-Rosales DJ, Medrano-Roldán H, Reyes-Jáquez D. 2020. Effect of lipids content and process parameters on the physicochemical, rheological, calorimetric and structural properties of an extruded canine food. Animal Nutrition and Feed Technology, 19, 455-469. DOI: 10.5958/0974-181X.2019.00042.8.

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Melissa M. Melough, Shaowei Wu, Charles Eaton, Abrar A. Qureshi, Ock K. Chun, Eunyoung Cho. Citrus Consumption and Risk of Cutaneous Malignant Melanoma in the Women’s Health Initiative. Nutr Cancer. 2020;72(4):568-575. doi:10.1080/01635581.2019.1644353.

Weiyi Sun, Megan S. Rice, Min Kyung Park, Ock K. Chun, Melissa M. Melough, Hongmei Nan, Wenqing Li, Abrar A. Qureshi, Eunyoung Cho. Intake of Furocoumarins and Risk of Skin Cancer in Two Prospective US Cohort Studies. J Nutr. 2020;00:1–10.  https://doi.org/10.1093/jn/nxaa062

Evans R, Engelhorn HJ, Winger QA, Chicco AJ, Bouma GJ. A multiplex PCR genotyping assay to distinguish XX and XY tissues in sheep. Mol Biol Rep in press. PMID:  32743776

Haugen Frenkel JD, Ackart DF, Todd AK, DiLisio JE, Hoffman S, Tanner S, Kiran D, Murray M, Chicco AJ, Obregon-Henao A, Podell B, Basaraba R. Metformin enhances protection in guinea pigs chronically infected with Mycobacterium tuberculosis. Sci Rep in press

Le CH, Benage LG, Specht KS, Li Puma LA, Mulligan CM, Heuberger AL, Prenni JE, Claypool SM, Chatfield KC, Sparagna GC, Chicco AJ. Tafazzin deficiency impairs CoA-dependent oxidative metabolism in cardiac mitochondria. J Biol Chem, 295(35):12485-12497, 2020. PMID:  32665401

Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ. Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Reg Integr Comp Physiol, 318(5):R972-R980, 2020. PMID: 32233925

 

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