Brief Summary of Minutes of Annual Meeting

NE-1640 Regional Nematology Research Committee meeting Minutes,

Via video conference, Oct. 21-22, 2020

Marisol Quintanilla, Chair. 

Administrative Advisor:  Mark Rieger

Attending: Andreas Westphal (California), Jim LaMondia (CT), Billy Crow (UFL), Koon-Hui Wang (U-Hawaii), Marisol Quintanilla (MSU), George Bird (MSU), Amanda Howland (MSU), Sita Thapa (MSU), Luisa Parrado (MSU), Nathaniel Mitkowski (URI), Ernie Bernard (Tenn), Debra Neher (Vermont), Jim Kotcon (WVU), Brandon Edgar (WVU), Carmen Ugarte (IL), Lesley Schumaker (USDA-ARS-Tenn), Chris Taylor, (OH), Robb Wick (UMass), Mark Rieger (Administrative Advisor) .

The 2020 NE-1640 meeting was held via video conference due to the COVID-19 pandemic on October 21^st and 22^nd  2020 from 1 to 5 pm on both days.

Administrative Advisor

Mark Rieger commended our great report.  He recommended that we write an application for a Multistate research award and said we are well positioned to obtain one. 

Regarding the topics to consider for objectives, he said that we were right on with nematode assessment and that we could almost see the future ahead of time five years ago.  He mentioned soil health as important and specifically mentioned the importance of SCN.  He will send email with deadlines and will give us contact of David Leibovitz

Presentations

Ernest Bernard (Tennessee) reported results from trials evaluating varietal responses of CBD hemp cultivars to root know nematodes (Meloidogyne incognita).  Susceptible cultivars include ‘Charlottes Web’ and ‘Special Sauce’; ‘Siskiyou Gold was slightly susceptible; and ‘Carolina’ was resistant.  A statewide survey of solanaceous crops (137 soil samples) revealed that that M. incognita was present in 38 of 137 samples (28%), M. hapla was found in 3 samples and a mix of the two in 4 samples. The invasive species M. enterolobii was not collected from any sample. Surveys of vineyards found that 15 of 51 samples (29%) contained Xiphinema americanum-group dagger nematodes. DNA analysis for species identification is ongoing.  Commercial boxwood growers in Tennessee are seeing increasing infestations of Meloidogyne incognita. Greenhouse experiments have demonstrated the presence of resistance to the nematode in at least one boxwood cultivar.  Root galling in boxwood may lead to rejection of entire lots.

 William Crow (Florida) evaluated experimental nematode control treatments for turfgrasses and ornamental plants in eight greenhouse trials and 15 field trials.  Two field trials quantifying damage caused by lance nematode to bermudagrass golf greens were continued.  Nematicides were largely ineffective for controlling lance nematode (Hoplolaimus), but gave some benefit for other nematodes.  The foliar nematodes Aphelenchoides besseyi, A. oryzae and A. pseudobesseyi were recognized, and A. pseudobesseyi may be an emerging problem on soybeans as symptoms are easily confused with stink bug injury.  Incidence of the awl nematode (Dolichodorus) on turfgrass is increasing, this nematode reproduces well on the cover crop sunn hemp and the weed purple nutsedge.

Nathaniel Mitkowski (Rhode Island) demonstrated the efficacy of fluazaindolizine, but not fluopyram and abamectin, against Hoplolaimus galeatus on golf course putting greens where populations regularly reached 7,000 nematodes/100 cc soil.  While lower rates of fluazaindolizine produced a non-significant but possibly biologically detectable drop in nematode populations, higher rates of the material resulted in statistically significantly reductions in nematode population and statistically (and visually) improved turf quality.  While abamectin has been reported to have an effect on H. galeatus in other studies, it was unclear why it was ineffective in this trial.  Fluopyram has not been reported as effective against H. galeatus previously but it does work well on Tylenchorhynchus spp. and Meloidogyne in Northern states.

Chris Taylor (Ohio) surveyed root knot nematode populations in high tunnel tomato production and detected M. hapla in 45% of 71 high tunnels sampled.  Two biocontrol products (Actinovate and Bio-Activate) produced modest reductions in nematode numbers in greenhouse trials, but did not influence galling or yield .in microplots.  A large collection of Pseudomonads were screened for nematicidal activity in vitro, but these failed to reduce soybean cyst nematode numbers in microplots.

Marisol Quintanilla evaluated nematode control strategies such as nematicides, composts, and cover crops for crops such as corn, soy, sugar beets, potatoes, vegetables (carrots), fruits, ornamentals (daylilies).

Lesley Schumacher (USDA-ARS, Tennessee) described studies she is initiating on soybean cyst and lesion (Pratylenchus penetrans) nematodes in soybeans.

Rob Wick (Massachusetts) described a field trial of a proprietary compound for nematode control in turf grass.  He also is describing a new species of root knot nematodes from turf grasses, and reported a finding of Bursaphelenchus antoniae on white pine.

Carmen Ugarte (Illinois) described surveys for abundance and community structure of nematodes in organic grain production.  Soybean fields in farms with long rotations had lower population densities of soybean cyst nematode, and soils had greater suppressiveness than from farms using shorter rotations.

Debra Neher (Vermont) described work using anaerobic soil disinfestation for suppression of soil borne diseases.  Soils with complex food webs containing fungi and fungivorous nematodes were correlated with greater suppression.

Koon-Hui Wang (Hawaii) reported on biofumigation with Brassica and papaya for control of root knot nematode.  Additional studies included sorghum and sunn hemp biomass.

George Bird (Michigan) found increased potato yields, but no differences in nematode populations using black oats as a trap crop.  Using a PI437654-based soybean cultivar as a trap crop found no HG Type 1.2 SCN reproduction on this seed under chamber condition.  The Soybean Cyst Nematode Working Group found that 80 % of soybean growers do not sample for soybean cyst nematodes.

James Lamondia (Connecticut) evaluated P. penetrans reproduction on legumes used as cover crops and found that purple clover and partridge pea were suppressive.  A solanaceous weed, Solanum sisymbriifolium (sticky nightshade or Litchi tomato) was more suppressive than a resistant tobacco cultivar to tobacco cyst nematode (Globodera tabacum) which is being used as a model for potato cyst nematodes G. pallida.  A new Beech Leaf Disease associated with Litylenchus nematodes appears to be widespread in New England, with high nematode numbers defoliating trees.

Frank Hay (Cornell) and Sarah Pethybridge have developed primers specific to M. hapla DNA isolated directly from soils.  Heat treatment of bulbs at 48 C for 30 minutes controlled Ditylenchus dipsaci in garlic.

James Kotcon (West Virginia) is evaluating nematode trapping fungi for control of dagger nematodes (Xiphinema spp.) in peach orchards.  A new experiment will assess changes in soil microbial communities inoculated with bacteriovore, fungivore, and carnivore nematodes.  Whole microbial community DNA will be extracted from soils before and after incubation with nematodes, and then sequenced to estimate changes in both bacterial and fungal community composition, diversity and abundance.

Andreas Westpahl (California) observed differences in reaction to Pasteuria isolates and resistance genes in pepper to differentiate races of Meloidogyne hapla, which will allow growers to plant inherently resistance crop species and varieties to preserve crop yield and grower profitability.  Experiments utilizing poultry based compost enhanced the survival of enteric pathogens in soil more than dairy-based compost.

Business meeting

Jim Kotcon was elected Chair, and Ernie Bernard secretary for the coming year

Jim Kotcon moved to accept the invitation to meet in Florida’s southwest region in September 2021.  Southwest Florida regional airport (RSW) is the closest airport

The project is in its final year, and approval to re-write has been received.  There was a consensus to include new subject matter and a new title.  The project will involve soil health as a cross-cutting approach, particularly the biological side of soil health with special reference to nematodes.

Other topics discussed include nematode distribution and emergence of new pests, and how climate change and how is that affecting distribution, i.e.  Ditylenchus, Litylenchus, etc.

Proposed Title: Sustainable Management of Nematodes in Soil and Plant Health Systems

Objectives will include: 1. Nematode management, 2. Nematode ecology, 3.  Detection, distribution, and movement of invasive and emerging nematode pests, and 4.  Outreach to the agricultural community and general public

Committee:  One of them did the critical review, look at the regional projects, 3 or 4 in the nation.  We do not want mayor overlap with regional projects. 

Chairs for objectives: Andreas Westphal (management), Jim Kotcon (ecology and soil health objective), Billy Crow (invasive pest, movement and distribution), Marisol (extension outreach), Four people in the rewrite committee, one is responsible for the critical review to make sure there no major regional overlap. 

Proposed Writing Schedule:  November 1^st, members respond with their paragraphs, the committee has first draft by Nov 10^th, get a final draft by Nov 15^th.  A Temporary number 2140 was assigned. 

The meeting adjourned at 4:12 PM.

Respectfully submitted by:

Jim Kotcon, West Virginia University

SHORT-TERM OUTCOMES:

CA: In 2020 CA, elites of the walnut rootstock development program were forwarded into commercial-scale field trialing.

HI: Over three field trials, we found fluopyram is effective against Meloidogyne spp. whereas azadirachtin is more effective against Rotylenchulus reniformis. Integrating fluopyram with sunn hemp cover cropping or even azadirachtin chemigation might be necessary to manage Meloidogyne spp. and R. reniformis concurrently. The integration of cover cropping with fluopyram or azadirachtin chemigation significantly reduced the negative impact of these nematicides on soil health.

IL: Regional sampling for SCN across organic farming operations revealed that systems managed with long-term rotations (i.e., > 3-yrs) may promote SCN suppression as oppose to short-term rotations (i.e., 3-yr rotation).

FL: Evaluated experimental nematode control treatments for turfgrasses and ornamental plants in 8 greenhouse trials and 15 field trials. Conducted second year of two field trials quantifying damage caused by lance nematode to bermudagrass golf greens.

MA: Because of COVID and severe restrictions of undergraduates working in labs or outdoors, it was not possible to carry out any meaningful field plot trials this past growing season. One field trial of a proprietary material to control nematodes in turfgrasses was completed but lack of rain throughout the growing season prevented the nematode population from developing a normally robust population so that differences in treatments and control were not apparent.  

MA: We continue to make progress in collecting morphometric data on a new species of root-knot nematode from a Poa/Agrostis stand from two different golf courses in northern New Hampshire.

TN: Medicinal (CBD) hemp cultivars have a wide range of reactions to Meloidogyne incognita, from highly susceptible to highly resistant. Should this nematode become a significant field pest of hemp, resistant cultivars already exist for field planting or development of new lines.

TN: Commercial boxwood growers in Tennessee are seeing increasing infestations of Meloidogyne incognita. Greenhouse experiments have demonstrated the presence of resistance to the nematode in at least one cultivar.

TN: A statewide survey of solanaceous crops (137 soil samples) revealed that that M. incognita was present in 38 of 137 samples (28%), M. hapla was found in 3 samples and a mix of the two in 4 samples. The invasive species M. enterolobii was not collected from any sample.

TN: In a statewide survey of vineyards for pests and pathogens, 15 of 51 samples (29%) contained Xiphinema americanum-group dagger nematodes. DNA analysis for species identification is ongoing.

RI: Golf course superintendents will have a new, highly effective nematicide with less environmental impact, for use on golf courses with the registration of fluazaindolizine, currently in the EPA pipeline. The material is extremely efficacious at reducing populations of Hoplolaimus galeatus, one of the most aggressive nematodes on turf in the northern states. No other currently labeled turf nematicide has activity against H. galeatus. The material has also shown efficacy on Tylenchorhynchus spp. and Helicotylenchus spp.

VT: Replicated on-farm trials of two approaches aimed toward reducing inoculum load of Rhizoctonia solani for lettuce production: anaerobic soil disinfestation and blending vermicompost in starter mix. These trials will be repeated in 2020 and 2021. These projects are funded by a combination of small grants from the Northeastern Sustainable Agriculture Research and Education, Organic Valley’s Farmers Advocating for Organic, a Vermont Specialty Crop Block Grant. The project constitutes the thesis research of Master’s student, Anna R. Brown.

OUTPUTS:

CT: Dr. LaMondia conducted 172 nematode diagnostic samples and conducted testing as an APHIS certified pinewood nematode export testing facility.

FL: The Florida nematode assay lab provided diagnoses on 5,340 samples submitted to the lab. Thirteen presentations were given in-person or via webinar to grower groups.

RI: Approximately 200 nematode samples were processed from golf course putting greens to determine if damaging levels of plant parasitic nematodes were present and whether control of these populations were warranted. Five presentations were given to golf course superintendents and other professionals regarding nematode management.

Many peer reviewed publications were produced by collaborators of the project, see additional list.

ACTIVITIES:

Objective 1. Develop and integrate management tactics for control of plant-parasitic nematodes including biological, cultural (such as rotation or cover crops and plant resistance), and chemical.

CA: Field experiments for a winter cover crop period are established. Two cover crop treatments (one including brassica, the second Merced Rye) show promise in either reducing nematode population densities or improving plant growth. Experiments were harvested for almond production in 2020.

CT: We have previously had success in managing plant parasitic nematodes using rotation crops but continue to look for plants with value beyond impact on nematode populations. We conducted experiments to screen a range of leguminous plants used for pollinator forage for suppression of lesion nematodes in microplots. We planted Purple clover, White clover, Creamy milk vetch, Round bush clover, Slender bush clover, Senna, Pannicled tick trefoil, and Partridge pea in comparison with Oats and Black oats.

CT: Trap crops are being developed for nonchemical control of cyst nematodes. A solanaceous weed, Solanum sisymbriifolium (sticky nightshade or Litchi tomato) is being evaluated to control potato cyst nematodes Globodera pallida. Because of the difficulties in working with this regulated pathogen, we used the closely related tobacco cyst nematode G. tabacum as a model system. Experiments were conducted to evaluate S. sisymbriifolium for ability to stimulate hatch of G. tabacum in comparison to a susceptible or resistant host plant, for ability of the nematode to reproduce and increase, and for efficacy against the nematode as a trap crop under field conditions in comparison to plant resistance.

HI: In a Sorghum/Sorghum-Sudangrass hybrids (SSgH) trial to screen for effective cover crop as soil builders and microbial enhancers, an energy sorghum (NX-D-61/5D61) was most promising as it produced the highest amount of cover crop biomass (higher than sunn hemp, a popular green manure crop in Hawaii), increased soil carbon content and conserved soil moisture at the end of 2.5 months of growth among 12 varieties tested. NX-D-61 used as soil amendment was also most suppressive to root-knot nematodes based on a mustard green host bioassay in a pot experiment. Rhizosphere soil from NX-D-61 along with another sorghum-sudangrass hybrid, Lattee, yielded higher microbial biomass compared to bare ground and most of the other varieties tested based on phospholipid lipid acid profile analysis. Through multivariate analysis using Canoco for Window 10 software, we found a positive relationship between microbial abundance with SSgH biomass, soil moisture, soil microbial respiration, soil C, but a negative relationship with abundance of plant parasitic nematodes.

MI: Seed for a PI 437654-based SCN trap crop has been increased for two years under field conditions in cooperation with a local seed company. There was no HG Type 1.2 SCN reproduction on this seed under chamber conditions. SCN trap crop seed has been distributed to eight commercial soybean growers for late 2020 summer planting and evaluation of its impact on soybean yields in 2021.

MI: In a three-year seed potato production system, two years of an eight-cultivar cover crop blend resulted in a 31.7% greater potato tuber yield compared to the current cover crop practice (P <0.05).     

OH: Examination of root-knot nematode in tomato production systems in Ohio. High tunnel production is an emerging production system for tomatoes in Ohio that allows earlier and later production of fresh market tomatoes (+2 months). Plant and soil samples were collected from 36 farms from across Ohio. Plant samples were examined visually for the presence of root-knot nematode and soil samples were planted with susceptible tomato (cv. Moneymaker) and later examined for the formation of knots. Out of the 71 high tunnel plants/soils examined the presence of Meloidogyne hapla was detected in 32 samples (45%) and of the 36 farms examined, 19 (52%) had M. hapla thus confirming the presence of M. hapla in 14 counties in Ohio. Using PCR diagnostics, 26 of the plant samples tested positive for M. hapla and 2 tested positive for M. incognita. DNA obtained from soil samples provided 6 additional positive M. hapla samples.

OH: Two different M. hapla populations (one from lettuce collected previously from muck soils and one from a high-tunnel under tomato production) and one M. incognita population (also collected from a high-tunnel under tomato production) were examined for their ability to affect yield. Over 200 microplots were planted in a high tunnel and different inoculum levels were used. Microplots were treated with either 500, 5000, or 25000 eggs from each of the three populations and a non-inoculated control utilizing a randomized block design. There was no difference in yield or number of fruits per plant across all treatments.

OH: Nine different commercial biocontrol products were examined for their ability to control root-knot nematode (M. hapla and M. incognita) under greenhouse and microplot (high-tunnel) conditions. Only two biocontrol products (Actinovate AG and Bio-Activate) showed a modest reduction in root-knot nematode numbers under greenhouse conditions. In microplots, none of these nine products tested significantly altered the gall index nor did it alter yield or total number of fruits per plant.

RI: During the 2020 field season, nematicide trials were undertaken on golf courses in Rhode Island to compare the efficacy of fluazaindolizine, fluopyram and abamectin against Hoplolaimus galeatus.   Neither fluopyram nor abamectin, singly or in combination, were effective at managing H. galeatus on golf course putting greens where populations regularly reached 7,000 nematodes/100 cc soil. While lower rates of fluazaindolizine produced a non-significant but possibly biologically detectable drop in nematode populations, higher rates of the material resulted in statistically significantly reductions in nematode population and statistically (and visually) improved turf quality. While abamectin has been reported to have an effect on H. galeatus in other studies, it was unclear why it was ineffective in this trial. Fluopyram has not been reported as effective against H. galeatus previously but it does work well on Tylenchorhynchus spp. and Meloidogyne in Northern states.

TN: The cultivar ‘Wife’ was confirmed to be nearly immune to M. incognita and ‘Carolina’ was a poor host, whereas roots of ‘Charlotte’s Web’ were consistently heavily galled and supported high egg production. Other tested cultivars (‘Cherry’, ‘OG’) were intermediate in suitability for nematode galling and reproduction. ‘Special Sauce’ was a good host for M. incognita, ‘Frosted Lime’ was intermediate and ‘Siskiyou Gold’ was a poor host when measured in terms of egg production. In a small test with M. hapla, ‘Wife’ was a moderately good host but ‘Special Sauce’ was highly resistant. Regardless of host, galls were small, white and hard; hand-sectioning revealed strong production of columnar vascular parenchyma in galled root tissue. In the boxwood study, distinct differences occurred in galling between the various cultivars with one cultivar being nearly free of galling. Planting medium also had a strong effect on symptoms; galling was most severe on plants in sand, less severe in 1:1 sand:peat, and almost non-existent in peat and bark mulch.

Objective 2. Determine the ecological interactions between nematode populations, nematode communities, ecosystems and soil health.

CA: Anaerobic soil disinfestation is tested in various experimental contexts to determine the method’s limitations. Sites infested with different nematode species and following different crops were used for testing of substrate amount and irrigation regiment.

IL: Participated in the design of a survey to estimate the abundance and community structure of plant parasitic and free-living nematodes in fields managed for commercial organic grain production in Illinois (Collaborating PIs: Jaeyeong Ham (PhD Candidate), Carmen M. Ugarte, Nathan E. Schroeder, and Glen L. Hartman)

MI: In 2017-2019, thermo-stability information obtained from remote sensing and hand dug geo-positioned tuber yields show that tuber yields under cool and stable conditions were greater than those under hot and stable or sites with thermo-instability. Thermo-stability is being proposed as a new soil health indicator.

OH: A large collection of pseudomonads was examined for their ability to affect root-knot and soybean cyst nematode. Under in vitro conditions, numerous Pseudomonas strains were identified that could affect nematode viability. Further in planta experiments in the greenhouse narrowed down the activity to nine different strains. However, three years of microplot experiments has shown that none of these were able to limit SCN numbers under the conditions tested. Further in vitro experiments showed that volatiles produced by certain strains of Pseudomonas could kill RKN and SCN. Subsequent analysis of volatiles has revealed that the production of hydrogen cyanide and organosulfur compounds can be lethal to these nematodes. Current work is focused on examining how to provide the necessary substrates that lead to the production of these nematode-lethal volatiles in soil to determine if better control can be achieved.

TN: An extensive paper on decomposition of beaver carcasses was published (Taylor et al. 2020). Nematode succession and community composition were much different under human cadavers than under the beaver cadavers of the previous experiment. Nematodes are ubiquitous in soil and sensitive to sudden and large inputs of nutrients that support fungal and bacterial growth. Forensic nematology studies with both beaver and human cadavers strongly suggest that nematode communities under corpses can be useful in estimating post-mortem intervals, especially in cases where a body has been undisturbed for weeks or months.

Objective 3. Outreach and communication - Compile and present/publish guidance on nematode management and management effects on soil health for different crops under different conditions.

CT: As a part of diagnostic services, we confirmed the first report of beech leaf disease caused by the foliar nematode Litylenchus crenatae subsp. mccannii in southwestern Connecticut in 2019, in south eastern CT, Rhode Island and Plymouth MA in 2020. Educational programs will be directed to arborists in the state.

HI: We presented nematode and soil health management projects through guest lectures to new farmers’ training program, displays at Ag Day at the Capital to state legislators, farmers and other ag-professionals, displays at College of Tropical Agriculture and Human Resources (CTAHR) Day to high school student visitors, or at the University of Hawaii Manoa student recruitment day. Two YouTubes videos were generated: one on Cover Crop for Soil Health Management, the other one on use of entomopathogenic nematodes and trap cropping for diamondback moth management. Four peer reviewed and 4 extension articles were published to share our results findings. A “Virtual Soil Health and Sustainable IPM Mini Conference” was presented to 30 participants.

MI: In 2019, the SCN Coalition sponsored 239 winter meetings, 77 field days and 39 train the trainer events. It also had circa 10,000 direct grower contacts, 117 local media events and published 25 Extension documents. In addition, Dr. Bird received a major national public relations award.

TN: A comprehensive summary of major hemp diseases and pests in Tennessee was published (Hansen et al. 2020).

VT: Podcast Episode, A Soil Symphony, where Dr. Deborah Neher tells the story of compost as biologically rich soil, https://open.spotify.com/episode/1FMVZTGYhljRii7Ydy5GFM

VT: Curriculum Development Contractor for the Composting Association of Vermont. Soil builders – education for action: Using compost to prevent erosion and improve water quality in the Lake Champlain Basin, $40,000, May 2020 – May 2021.

ACOMPLISHED MILESTONES (2020):

CT: Conclude and evaluate long-term impacts of cover - and rotation-crop experiments

MA: A 3-hour workshop on the identification of endoparasitic plant parasitic nematodes was carried out on March 10, 2020 at the University of Massachusetts. There were 14 diagnosticians representing the Northeast Plant Disease Network in attendance.

OH: Analyze and publish location-specific traits relative to M. hapla populations

TN: Conclude and evaluate long-term impacts of cover- and rotation-crop experiments.

Multiple States: Determine the relationship between the microbial community, plant-parasitic nematodes, soil health, and crop productivity

Multiple states: Publish preliminary (and final) results

Multiple states: Conduct grower education, annual short course and webinar

Adams, A., J. LaMondia, R. Cowles, B. Nicholson and T. Mione. 2020. Stimulating hatch of tobacco cyst nematode Globodera tabacum, by hydroponically obtained weedy Solanum spp. root exudates. Nematropica in press.

Bintarti, A.F., Wilson, J., Quintanilla-Tornel, M., and Shade, A.   2020. Biogeography and diversity of multi-trophic root zone microbiomes in Michigan apple orchards: analysis of rootstock, scion, and growing region. Phytobiomes Journal.  

Cole, E., Pu, J., Chung, H., and Quintanilla, M. 2020. Impacts of manures and manure-based composts on root lesion nematodes and Verticillium dahliae in Michigan potatoes. Phytopathology.

Crow, W. T., Habteweld, A., Bean T. 2020. Mist chamber extraction for improved diagnosis of Meloidogyne spp. from golf course bermudagrass. Journal of Nematology 52:e2020-96.

Demesyeux, L., Mendes M. L., Crow, W. T., Chambers, A. H. 2020. Plant-parasitic nematodes associated with eight banana cultivars in southern Florida. Nematropica 50:19-28.

Dyrdahl-Young, R., Cole, E., Quintanilla Tornel, M., Weldon, R., & DiGennaro, P.   2020. Economic assessment of nematode biological control agents in a potato production model. Nematology, 1(aop), 1-9.

Eberlein, C., Heuer, H., and A. Westphal. 2020. Biological suppression of populations of Heterodera schachtii adapted to different host genotypes of sugar beet. Frontiers in Plant Science. Plant Pathogen Interactions. Online: https://doi.org/10.3389/fpls.2020.00812 https://www.frontiersin.org/articles/10.3389/fpls.2020.00812/full?

Eberlein, C., Edalati, A., Zhang, R., and A. Westphal. 2020. A rapid bioassay for measuring nematode suppressive potential of anaerobic digestate. Nematology 22: 879-889.

Eshchanov, Bahodir and George Bird. 2020. Influence of grafting and pruning on Solanum lycopersicum ‘Anahu’ and ‘Rutgers’ biomass partitioning in the presence and absence of Meloidogyne incognita (Nematoda). Acta Horticulturae (in press).

Habteweld, A., Akyazi, F., Joseph, S., Crow, W. T., Abebe, E., Mekete, T. 2019. Description of Hirschmanniella dicksoni n. sp.(Nematode Pratylenchidae) from rhizosphere soil of limpograss from Florida U. S. A. Journal of Nematology 51:e2019-83.

Hansen, Z. R., E. C. Bernard, J. F. Grant, K. D. Gwinn, F. A. Hale, H. M. Kelly, and S. D. Stewart. 2020. Hemp Disease and Pest Management. University of Tennessee Extension Publication, W 916. 15 pp.

Jones, W. B., Kruse, J. K., Enloe, H. A., Crow, W. T. 2020. Effects of pre-planting incorporation or post-planting top-dressing of organic amendments on bermudagrass for tolerance to Belonolaimus longicaudatus. Nematropica 50:59-66.

Marra R. E. and J. A. LaMondia 2020. First Report of Beech Leaf Disease, caused by the foliar nematode, Litylenchus crenatae, on American Beech (Fagus grandifolia) in Connecticut. Plant Disease 104: https://doi.org/10.1094/PDIS-02-20-0442-PDN.

Mendes, M. L., Dickson, D. W., Crow, W. T. 2020. Yellow and purple nutsedge and coffee senna as hosts of common plant nematodes in Florida. Journal of Nematology 52:e2020-94.

Miller, M., Uppala, L., and Wick, R. 2020. A Survey of Plant Parasitic Nematodes in Cranberry Bogs in Massachusetts (Poster, NEDAPS)

Myers, R., B. Bushe, C. Mello, J. Lichty, A. Hara, B. Sipes, and K.-H. Wang. 2020. Yield Increases in burrowing nematode infested anthurium with fluopyram and trifloxystrobin applications. HortTech 30: 603-607 (doi.org/10.21273/HORTTECH04648-2).

Neher, D.A. and Barbercheck, M.E. 2019. Soil microarthropods and soil health: Intersection of decomposition and pest suppression. Special issue on “Elucidating the Role of Soil Arthropods in Soil Health”, Insects 10(12):414. doi: 10.3390/insects10120414

Neher, D.A., Limoges, M.A., Weicht, T.R., Sharma, M., Miller, P.D. and Donnelly, C. 2020. Bacterial community dynamics distinguish poultry compost from dairy compost and unamended soils planted with spinach. Microorganisms 8(10):1601. doi:10.3390/microorganisms8101601

Nuaima, R.H., H. Heuer, and A. Westphal. 2019. Effects of cover cropping on microbial communities associated with Heterodera schachtii and nematode virulence. Soil Systems 3(4) 67. Online: https://doi.org/10.3390/soilsystems3040067

O’Brien, B.J., Neher, D.A., and Roy, E.D. 2019. Nutrient and pathogen suppression properties of anaerobic digestates from dairy manure and food waste feedstocks. Waste and Biomass Valorization doi: 10.1007/s12649-019-00906-4

Phillips, G., J. K. Moulton, and E. C. Bernard. 2020. Heth pivari n. sp. (Nematoda: Ransomnematoidea: Hethidae) from the indigenous North American millipede Narceus gordanus (Spirobolida: Spirobolidae), with keys for worldwide Heth spp. Zootaxa, 4861 (1): 486-514.

Porterfield, K.K., Joblin, R., Neher, D.A., Curtis, M., Dvorak, S., Rizzo, D.M., Faulkner, J.W. and Roy, E.D. 2020. Upcycling phosphorus recovered from anaerobically digested dairy manure to support production for vegetables and flowers. Sustainability 12:1139. doi: 10.3390/su12031139

Sedaghatjoo, S., Söchting, H.P., and A. Westphal. 2017. Potential of Crotalaria juncea as a cover crop for cyst nematode suppression under Central Europe conditions. Nematoda: published online DOI: 10.4322/nematode.00417.

Taylor, Lois S., G. Phillips, E. C. Bernard, and J. M. DeBruyn. 2020. Soil nematode functional diversity, successional patterns, and indicator taxa associated with vertebrate decomposition hotspots. PLoS ONE, 15(11): e0241777.

Ugarte, C.M., and J.R. Taylor. 2020. Chemical and biological indicators of soil health in Chicago urban gardens and farms. Urban Agriculture and Regional Food Systems. doi10.1002/uar2.20004

Waisen, P., Z. Cheng, B.S. Sipes, J. DeFrank, S.P. Marahatta and K.-H. Wang. 2020. Effects of biofumigant crop termination methods on suppression of plant-parasitic nematodes. Applied Soil Ecology 154: 103595 (https://doi.org/10.1016/j.apsoil.2020.103595).

Waller, R., Linderme, J and Wick, R. 2020. An Undescribed Meloidogyne sp. (root-knot nematode) from turfgrasses in two sites in New Hampshire (Poster, NEDAPS)

Westphal, A., Maung, Z.T.Z., Doll, D.A., Yaghmour, M.A., Chitambar, J.J., and S.A. Subbotin. 2019. First Report of the Peach Root-Knot Nematode, Meloidogyne floridensis Infecting Almond on Root-Knot Nematode Resistant “Hansen 536” and “Bright’s Hybrid 5” Rootstocks in California, USA. Journal of Nematology, 51, 1–3. https://doi.org/10.21307/jofnem-2019-002

Thesis: Rebecca Kimmelfield: (PhD Thesis; 2020, OSU, Plant Pathology): Establishing the use of Pseudomonas spp. as biocontrol agents of fungal and nematode pathogens.

Thesis: Marlia Bosques Martinez: (Masters of Science; 2020, OSU, Plant Pathology): Assessment of root-knot nematode presence in tomatoes in Ohio, yield loss, and biocontrol.