SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Participant List (only include members and visitors that attended the annual meeting) Geisert, Rod; University of Missouri Hamernik, Deb; University of Nebraska-Lincoln Hines, Elizabeth A; Penn State University Feugang, Jean M.N.; Mississippi State University Knox, Rob; University of Illinois Lugar, Drew; Illinois State University Maddox, Carol; University of Illinois Miles, Jeremy; USMARC Miller, David; University of Illinois Parrish, John; University of Wisconsin Rempel, Lea; USMARC Ross, Jason; Iowa State University Safranski, Tim; University of Missouri Stewart, Kara; Purdue University White, Brett; University of Nebraska-Lincoln Visitors: Hamilton, Christina; NCERA Assistant Director Mathew, Alan; Purdue Animal Science Department Head Minton, Amanda; The Mashoffs

Brief Summary of Minutes of Annual Meeting

Meeting called to order on Tuesday, May 19th at 9:00 am CST via WebEx by institutional host, Dr. Kara Stewart. Business meeting immediately commenced. Due to the COVID19 pandemic, the scheduled symposium was postponed until next year and it was decided that it would be held mid-May of 2021 at Purdue University. The committee members thanked Kara Stewart for organizing the symposium and are looking forward to the symposium next year.

Recruitment was discussed, Brett White agreed to reach out to Amy Schweitzer as potential new member. Others agreed to consider potential new members and reach out.

John Parrish will serve as secretary for 2021, as self-nominated and approved by committee members. Chair for 2021 will be Elizabeth Hines, as promoted from current secretary.

Extensive discussion was held among members regarding current state of the swine industry due to COVID19, with updates provided from Indiana, Missouri, Pennsylvania, and Iowa.

It was brought up that member Benny Mote was unable to attend due to family emergency, Kara Stewart offered to organize a card from the committee for Benny.

Meeting proceeded with update from Christina Hamilton. Brief notes: Chris encouraged the use of the North Central Regional Association (NCRA) State Ag Experiment Station Directors website (www.ncra-saes.org) as well as the use of impact writing resources for the group. These resources likely improve efficiency of reporting and in acquiring grant funding for the group. Brief overview of the Multistate Research System: Funded from the Hatch Act, at least 25% of Hatch funds need to be spent on multi-state projects. Project committees, organized through NIMSS, goal is to focus on multi-state collaborations in specified research areas. NCERA57 focus on swine reproductive physiology research, as well as dissemination of that research. North Central virtual Handbook: Guidelines for Multistate Research Activites, provides instructions for developing annual reports. Renewed emphasis from NIFA for ‘shorter reports that really showcase multistate portfolio and emphasize importance of work to stakeholders’. Reports due 60 days after annual meeting. Utilize template that was circulated for building station reports. Highly encouraged us to look into visiting with Multistate Research Fund Impacts (site) for assistance in writing powerful impact statements through workshop at our next meeting.

Station reports on research were given by the following parties: Brett White; Kara Stewart; Jeremy Miles; Rod Geisert; Guest- Amanda Minton; Elizabeth Hines; Jason Ross; Dave Miller; Rob Knox; John Parrish.

Discussion on current teaching climate was initiated by John Parrish regarding student and educational transition from in-person to online classes. Due to yet unknown circumstances with education and progression of COVID19 pandemic, open source online learning resources were discussed. Encourage collaboration on materials with possibility of restricted in-person lectures. If any insight on keeping students on task or aid in online classroom learning, please share. Rod Geisert and John Parrish offered to share video resources with the committee.

Discussion of pursuing funding sources was held, review of current needs of the swine industry in COVID19 and how experts on the team can best serve those needs. Some focus recommended to be placed on resiliency of food system as well as precision agriculture. Will revisit at a later date.

Adjourn 4:35 pm, CST.

Accomplishments

Accomplishments Supporting Objectives

  • In support of Objective 1, researchers determined boars with varicocele had decreased semen quality during and after a heat stress event (USMARC and UNL ).
  • In support of Objective 2 and 4, investigators reported on pubertal neuroendocrine development in gilts (USMARC and WVU ).
  • In support of Objective 3 and 4, a team of researchers evaluated the metabolite profile of uterine flushings during early embryo elongation in swine (USMARC and UNL ).
  • In support of Objective 3 and 5, SowBridge (IPIC) talk on Dealing with Delayed Puberty in Gilts. Expert Panel: R Knox, K. Stewart, and T. Safranski. SowBridge. Aug. 7, 2019.(Iowa State, Univ. Illinois, Purdue, Univ. Missouri).
  • In support of Objective 1 and 4, researchers identified potential biomarkers in sperm that may provide early indication of fertility reduction due to elevated environmental temperature (Univ. of Illinois, Purdue, Univ. Wisc).
  • In support of Objective 1 and 4, researchers developed predictors and mediation methods for sperm quality in agricultural species (Univ Wisc, Miss. State).
  • In support of Objective 1 and 3, researchers collaborated with industry on the implications of low-dose semen (Purdue, Univ. of Illinois).
  • In support of Objective 3, Experts hosted an all-day workshop at Purdue University for heritage Large Black swine breeders (Purdue, Univ. of Missouri).
  • In support of Objective 1, researchers validated a novel microscopic imaging approach for high-throughput evaluation of sperm quality (MSSTATE and University of Illinois, UC publication) and demonstrated the usefulness of nanotechnology-based approaches to improving sperm quality (MSSTATE and Wisconsin University).
  • In support of Objective 1, investigators from different stations examined the mechanisms underlying LH-independent testosterone production. (UNL)
  • In support of Objective 1 and 3, collaborators evaluated sperm kinematics in semen from GnRH-II receptor knockdown and littermate control boars. (UNL)
  • In support of Objective 2, research stations collaborated to compare endocrine profiles of GnRH-II receptor knockdown vs. littermate control gilts. (UNL)
  • In support of Objective 2 and 5, researchers created of vulva scoring scale and industry wide preliminary investigation into pelvic-organ-prolapse (POP) among sows(ISU)
  • In support of Objective 2 and 4, researchers evaluated the impact of heat stress, and potential heat-stress mitigation, on ovarian function and reproductive success (ISU)

Impacts

  1. • Identification of biological markers of semen quality improves long-term development of mitigation strategies to heat stress in boars
  2. • Improved understanding of puberty onset in gilts lead to long-term development of selection tools and improve selection rates for gilts in pig production
  3. • Metabolic profile of the uterine environment during early embryonic life provides clues to understanding and mitigation of early embryonic death
  4. • Biomarker identification critical step to developing prediction tools and testing for heat stress related infertility in boars
  5. • Combined, research and publications aid producers in trouble shooting poor conception rates in reproductive herd
  6. • Research on ovarian function under heat stress provides basic understanding to underlying mechanisms and potential mitigation of heat stress production losses
  7. • Vulva scoring to identify sows susceptible to POP allows producers to more rapidly identify and respond to sows at risk in the population
  8. • Techniques for improvement of sperm quality maximizes genetic potential and reduces waste through seasonal impacts to animal reproductive capacity
  9. • Semen quality research has provided nutritionally based mitigation strategies to producers across the U.S.
  10. • Identification of biomarkers of subfertility that can now be tested as a screening tool for young boars.
  11. • Collaborative workshop for Large Black swine breeders successfully transferred knowledge to heritage breeders on how to manage genetics and inbreeding in small herds in the US.
  12. • Identification of subfertile boars at a younger age would allow producers to focus resources on reproductively superior animals and market subfertile males prior to sexual maturity, significantly increasing their value.
  13. • Improved identification of fertile vs. subfertile boars would contribute more doses of semen sold per ejaculate, reducing the cost of production.
  14. • Novel factors may be determined to improve semen extenders in swine, extending the lifespan of sperm and decreasing the cost of semen doses.
  15. • Demonstration that GnRH-II and its receptor are important to sperm function, represents a potential avenue to improve semen preservation.
  16. • Proteome profiles of the follicular fluid during folliculogenesis indicate potential biomarkers to better understand and develop strategies for female fertility improvement.
  17. • Enhance development of novel technologies for sperm selection to improve male fertility.

Publications

Publications

Basic Science and Technology

  1. Gruhot, T.R., Rempel, L.A., White, B.R., Mote, B.E. 2020. The effect of varicocele on semen quality in boars exposed to heat stress. Translational Animal Science. 4(1):1-6. https://doi.org/10.1093/tas/txaa003.
  2. Kerns K, Sharif M, Zigo M, Miller D, Sutovsky S. 2020. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. Int J Mol Sci 21:2121.
  3. Rubessa, M., Feugang, J.M., Kandel, M.E., Schreiber, S., Hessee, J., Salerno, F., Meyers, S., Chu, I., Popescu, G., Wheeler, M.B. 2020. High-throughput sperm assay using label-free microscopy: morphometric comparison between different sperm structures of boar and stallion spermatozoa. Animal Reproduction Science. 106509. https://doi.org/10.1016/j.anireprosci.
  4. Sharif M, Kerns K, Sutovsky P, Bovin N, Miller. DJ Progesterone induces porcine sperm release from oviduct glycans in a proteasome-dependent manner. Submitted.
  5. Walsh, S.C., Miles, J.R., Yao, L., Broeckling, C.D., Rempel, L.A., Wright-Johnson, E.C., Pannier, A.K. 2020. Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation. Journal of Molecular Reproduction and Development. 87:174-190. https://doi.org/10.1002/mrd.23306.
  6. Boettcher AN, Kiupel M, Adur MK, Cocco E, Santin AD, Bellone S, Charley SE, Blanco-Fernandez B, Risinger JI, Ross JW, Tuggle CK, Shapiro EM. Human Ovarian Cancer Tumor Formation in Severe Combined Immunodeficient (SCID) Pigs. Front Oncol. 2019 Jan 22;9:9. doi: 10.3389/fonc.2019.00009. eCollection 2019. PMID: 30723704.
  7. Schultz, R., Serao, N.L., Ross, J.W. 2019. Genetic improvement of livestock, from conventional breeding to biotechnological approaches. In: Animal Agriculture:Sustainability, Challenges and Innovations. Eds. Bazer, F.W., Wu, G. In Press (Elsevier).
  8. Mayorga E.J., Renaudeau, D., Ramirez, B.C., Ross, J.W., Baumgard, L.H. 2019. Heat stress adaptation in pigs. Animal Frontiers 9:54-61.

Boar Performance

  1. Feugang JM, Rhoads CE, Mustapha PO, Tardif S, Parrish JJ, Willard ST, Ryan PL. 2019. Treatment of boar sperm with nanoparticles for improved fertility. Theriogenology 137:75-81.
  2. Hufana-Duran D, Duran PG, Venturina E, Peralta MD, Parrish JJ. 2019. Predicting bull fertility by sperm nuclear shape in water buffalo (bubalus bubalis). Thai J. Vet Med 49:75-81.
  3. Lugar, D.W., Harlow, K.E., J. Hundley, M. Goncalves, J. Bergstrom, and K.R. Stewart. 2019. Effects of increased levels of supplemental vitamins during the summer in a commercial AI boar stud. Animal 1-13. doi:10.1017/S1751731119001150

Genetics and Genomics

  1. Wijesena, H.R., Rohrer, G.A., Nonneman, D.J., Keel, B.N., Petersen, J.L., Kachman, S.D., Ciobanu, D.C. 2019. Evaluation of genotype quality parameters for SowPro90, a novel genotyping platform for swine. Journal of Animal Science. 97(8):3262-3273. https://doi.org/10.1093/jas/skz185.
  2. Lents, C.A., Lindo, A.N., Hileman, S.M., Nonneman, D.J. 2020. Physiological and genomic insight into neuroendocrine regulation of puberty in gilts. Domest Anim Endocrinol. 11:106446. doi: 10.1016/j.domaniend.2020.106446.

Sow and Gilt Performance

  1. Harlow, K., Ferreira, C.R., Sobreira, T.J.P., Casey, T. and K.R. Stewart.   Lipidome profiles of postnatal day 2 vaginal swabs reflect fat composition of gilt’s postnatal diet. PLoS ONE 14(9): e0215186. https://doi.org/10.1371/journal.pone.0215186
  2. Harlow, K., Suarez-Trujillo, A., Hedrick, V., Sobreira, T.J.P., Aryal, U.K., Stewart, K.R. and T. Casey. Temporal analysis of vaginal proteome reveals developmental changes in lower reproductive tract of gilts across the first two weeks of postnatal.  Sci Rep. 2019 Sep 13;9(1):13241. doi: 10.1038/s41598-019-49597-w. 
  3. Leonard, S.M., Xin, H., Brown-Brandl, T.M., Ramirez, B.C., Dutta, S., Rohrer, G.A. 2020. Effects of farrowing stall layout and number of heat lamps on sow and piglet production performance. Animals. 10:348. https://doi.org/doi:10.3390/ani10020348.
  4. Seibert, J.T., Adur, M.K., Schultz, R.B., Thomas, P.Q., Kiefer, Z.K., Keating, A.F., Baumgard, L.H., Ross, J.W. 2019. Differentiating between the effects of heat stress and lipopolysaccharide on the porcine ovarian heat shock protein response. J Anim Sci. 97:4965-4973. doi: 10.1093/jas/skz343. PMID: 31782954.
  5. Bidne KL, Romoser MR, Ross JW, Baumgard LH, Keating AF. 2019. Heat stress during the luteal phase decreases luteal size but does not affect circulating progesterone in gilts1. J Anim Sci. 97:4314-4322. doi: 1093/jas/skz251.
  6. Hines EA, Romoser MR, Kiefer ZE, Keating AF, Baumgard LH, Niemi J, Gabler NK, Patience JF, Haberl B, Williams NH, Kerr BJ, Touchette KJ, Ross JW. 2019. The impact of dietary supplementation of arginine during gestation in a commercial swine herd: I. Gilt reproductive performance. J Anim Sci. 97:3617-3625. doi: 10.1093/jas/skz233.

Teaching and Extension

  1. Boyd, R., Zier-Rush, C., Moeser, A., Culbertson, M., Stewart, K., Rosero, D., & Patience, J. 2019. Review: Innovation through research in the North American pork industry. Animal, 13(12), 2951-2966. doi:10.1017/S1751731119001915

 

Abstracts/Posters/Professional Presentations

  1. Adur, M.K., Romoser, M.R., Bidne, K.L., Schultz, R.B., Keating, A.F., Baumgard, L.H., Ross, J.W. 2019 Proteomic assessment of the intra-uterine secretome during the peri- implantation phase to understand effects of increased ambient temperature on the porcine heat stress 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.
  2. Corredor, F.A., Sanglard, L.P., Ross, J.W., Keating, A.F., Serao, N.L. 2019. Phenotypic and genomic relationships between vulva score and reproductive performance in first- parity sows. American Society of Animal Science Midwest Section Meeting. Omaha, March 11-13.
  3. Desaulniers, A., Cederberg, R., Knox, R., Lents, C.A., White, B. 2019. Use of genetic engineering to elucidate reproductive physiology of the boar. In: Missouri Physiological Society 2019 Annual Meeting Abstracts, 26Oct2019, Columbia, MO. pp. 6.
  4. Doungkamchan K, Parrish JJ, Miller DJ. 2020. Proteomic identification of molecular pathways and biomarkers related to porcine seasonal heat-induced infertility in males. 53rd Annual Meeting of the Society for the Study of Reproduction.
  5. Ebrecht, M.A., Ross, C.E., Cederberg, R.A., Lovercamp, K.W., Desaulniers, A.T., White, B.R. 2019. Effects of GnRH-II on spermatogenic function of swine. Proceedings of the 16th Annual Gilbert S. Greenwald Symposium on Reproduction and Perinatal Research. p. 44.
  6. Kerns K, Sharif M, Zigo M, Miller DJ, Sutovsky P. 2019. Sperm capacitation-induced zinc efflux is necessary for increased proteasomal activity and release from oviduct glycans of the sperm reservoir. 52th Annual Meeting of the Society for the Study of Reproduction.
  7. Kiefer, Z.E., Chipman, A.L., Studer, J., Johnson, C., Rademacher, C.J., Linhares, D.C.L., Ross, J.W. 2019. Update on Prolapses and Pig Survivability Initiative. James D. McKean Swine Disease Conference. Ames, Iowa. November 7-8.
  8. Kiefer, Z.E., Chipman, A.L., Studer, J.M., Koester, L.R., Showman, L., Keating, A.F., Schmitz-Esser, , Ross, J.W. 2019. Identification of putative factors associated with pelvic organ prolapse in sows during late gestation. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.
  9. Knox RV, Belstra BA, Willenburg KL, D Gómez-López DH, and Stewart KR.   Effects of number of sperm and site of uterine semen deposition on conception rate and number of embryos in weaned sows receiving a single fixed time insemination. IXth International Conference on Boar Semen Preservation. Sydney, Australia
  10. Mills, K., U.K. Aryal, T. Sobreira, A.M. Minton, T. Casey, K.R. Stewart. 2020. Shotgun proteomics reveal seminal plasma proteomes are reflective of boar reproductive performance. 2020 Midwest ASAS.
  11. Park, S.B., Gruhot, T.R., Liao, S.F., Rude, B.J., Mote, B.E., Feugang, J.M. 2019. Favorable seminal plasma environment to sperm fertility after L-arginine addition to boar diet. 42nd International Conference of Boar Semen Preservation (ICBSP), Hunter Valley, NSW, Australia.
  12. Rohrer, G.A., Cross, A.J., Keel, B.N., Brown-Brandl, T.M., Cassady, J.P. 2019. Genetic factors associated with changes in feeding behavior due to elevated temperature. In: Proceedings of the Plant and Animal Genome Conference, San Diego, CA, Jan. 12-15, 2019. pg. 8, W034. Available: https://www.intlpag.org/2019/images/pdf/2019/PAGXXVII-abstracts-workshops.pdf
  13. Ross, C.E., Choat, F.H., Plager, K.N., Desaulniers, A.T., Cederberg, R.A., Mills, G.A., White, B.R. 2019. Effect of GnRH-II and its receptor on boar semen quality. Reproduction, Fertility and Development. 32(2):201. https://doi.org/10.1071/RDv32n2Ab53.
  14. Ross, J.W., Hale, B.J., Seibert, J.T., Adur, M.K., Keating, A.F., Baumgard, L.H. 2019. Physiological mechanisms through which heat stress compromises reproduction in pigs. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.
  15. Schultz, R.B., Bidne, K.L., Romoser, M.R., Adur, M.K., Baumgard, L.H., Keating A.F., Ross, J.W. 2019. Effect of heat stress on the corpus luteum proteome during early pregnancy establishment in pigs. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.
  16. Sharp, K., Mills, K., Garcia, R., K.R. Stewart.   Impacts of various farrowing induction protocols in attended farrowings.  Midwest ASAS, Omaha, NE.
  17. Shirley, L.K., L.K. Shirley, M. Burgett,A.P. Schinckel, T. Field, R. Garcia, J.S. Johnson, K.R. Stewart, R.M. Stwalley. Evaluations of cooling pads on late lactation sows.  Midwest ASAS, Omaha, NE.
  18. Stewart, K.R., T. Casey, K. Mills, U.K. Aryal and A.M. Minton. Shotgun proteome anaylsis of seminal plasma proteins differentiate boars by reproductive performance.  ICBSP, Hunter Valley, Australia.
  19. Studer, J.M., Kiefer, Z.E., Keating, A.F., Baumgard, L.H., Olsen, K.M., Rambo, Z., Wilson, M.E., Rapp, C., Ross, J.W. 2019. Investigating the impact of manganese supplementation on corpus luteum function. 52nd Annual Meeting Society for the Study of Reproduction, San Jose, CA. July 18-21.
  20. Supakorn, C., Lents, C.A., Martinez, X., Vallet, J.L., Boyd, R.D., Rohrer, G.A., Dedecker, A.E., Stalder, K.J. 2019. Associations between day one piglet serum immunocrit ratio and subsequent growth during gilt development [abstract]. Journal of Animal Science. 97 (Supplement 2):146. Abstract 352. https://doi.org/10.1093/jas/skz122.258.
  21. Walsh, S., Miles, J.R., Wright-Johnson, E.C., Keel, B.N., Rempel, L.A., Pannier, A.K. 2019. Transcriptomic profiles of uniform populations of in vivo-produced spherical, ovoid, or tubular porcine embryos during the initiation of elongation. Reproduction, Fertility and Development. 32(2):152. https://doi.org/10.1071/RDv32n2Ab53.
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