SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

Participants: Kenneth White, group administrator (Utah State University), Rod Geisert (University of Missouri), Brett White (University of Nebraska Lincoln), Ken Bondioli (Louisiana State University), Kiho Lee (Virginia Tech), Jerry Bouma (Colorado State University), Curt Youngs (Iowa State University), Irina Polejaeva (Utah State University), Matt Wheeler (University of Illinois), Carol Keefer (University of Maryland), Xiuchun (Cindy) Tian (University of Connecticut), Young Tang (University of Connecticut), Pablo Ross (University of California Davis), Celina Checura (Clemson University), Jean Feugang (Mississippi State University). and Charles Looney (University of Arkansas),

Brief Minutes of the W4171 Technical Committee Meeting

Hilton Hotel, New York City, NY

January 16, 2020

 

 

The W4171 committee chair Anna Denicol (University of California, Davis), called the meeting to order at 8:10 am. In attendance were Kenneth White (Utah State University), Rod Geisert (University of Missouri), Brett White (University of Nebraska Lincoln), Ken Bondioli (Louisiana State University), Kiho Lee (Virginia Tech), Jerry Bouma (Colorado State University), Curt Youngs (Iowa State University), Irina Polejaeva (Utah State University), Matt Wheeler (University of Illinois), Carol Keefer (University of Maryland), Xiuchun (Cindy) Tian (University of Connecticut), Young Tang (University of Connecticut), Pablo Ross (University of California Davis), Celina Checura (Clemson University) and Charles Looney (University of Arkansas).

 

The meeting agenda and the minutes of the previous meeting were approved. The W4171 project administrator, Kenneth White (Utah State University) provided project guidance including:

 

  • Administrative process and potential options for the W4171 project team collaborator (non-PI) from institutions with no experimental station to attend the W4171 annual meeting.

 

  • The W4171 annual project report needs to be submitted to NIFA within 30 days after the annual meeting.

 

  • Discussion of the 2021 W4171 annual meeting time and location within the United States, as the 47th IETS will be hold in Peru in 2021.

 

Stations started providing their annual reports. Stations that reported in the morning meeting were University of Arkansas (Looney), University of California, Davis (Ross and Denicle), Colorado State University (Bouma), University of Connecticut (Tian and Tang), and University of Illinois (Wheeler).

 

The meeting was paused at 12:15pm for lunch break, and continued in the afternoon at 12:35pm.

 

Stations that reported in the afternoon meeting were Iowa State University (Youngs), Louisiana State University (Bondioli), University of Maryland (Keefer), University of Nebraska Lincoln (White), Clemson University (Checura), Utah State University (Polejaeva), and Virginia Tech (Lee).

 

Discussion about potential collaborations between stations followed after the individual presentation.

 

Celina Checura (South Carolina) was nominated to serve as Secretary for the W4171 Technical Committee in 2020. Ken Bondioli (Louisiana State University) made a motion to approve the nomination and Irina Polejaeva (Utah State University) seconded the motion. The motion to elect Celina Checura (South Carolina) as Secretary was unanimously approved. Young Tang will serve as Chair for the W4171 Technical Committee in 2020.

 

The 2021 meeting of the W4171 group was discussed. It was tentatively agreed to be hold in St. Louis, Missouri in July/August 2021 and be concurrent with the 53rd Annual Meeting of the Society for the Study of Reproduction (SSR). Other possible locations discussed were South Carolina, Reno (Nevada), and University of California, Davis. The exact meeting location and time will be determined within the following 3 - 4 months.

 

The meeting was adjourned at 3:00 pm.

 

 

Respectively Submitted,

 

Young Tang - Secretary

W4171 Technical Committee

Accomplishments

Accomplishments:

 

Objective 1: Understand the biology of gamete development, fertilization and embryogenesis including the underlying cellular and molecular mechanisms.

 

  1. Validation of antibodies against FSHR and p42/44 MAPK for use in bovine cells.

Characterization of gene expression in bovine PGCs.

 

  1. Determined the requirement of miRNA for bovine preimplantation development. Determined the effect of Magnesium in bovine preimplantation development.

 

  1. Systematically characterized the epigenetic landscape (DNA methylome and chromatin modifiers) of the bovine in vivo developed pre-implantation embryos. Characterized the epigenetic phenomenon, X chromosome dosage compensation in the sheep.

 

  1. The combination of concentration-exposure time to cilostamide during IVPM delayed meiotic progression in bovine after 6 and 12 h of culture. However, overall the culture period (IVPM+IVM) influenced the oocyte chromatin configuration and kinetics in both goats and cattle.

 

  1. The bovine genome was mapped to determine specific areas where gene promoters and/or enhancers were most accessible. These regions changed in a stage specific manner and correlated with stage specific expressed genes.  Accessible chromatin regions were reveled near the transcription start sites of active genes as well as at distal regions representing transcription factor binding motifs.

 

  1. The effect of vitrification at the blastocyst stage on gene expression in later day 14 elongated embryos was studied. Gene Ontology pathway analysis strongly suggested that mitochondrial function and energy production were impacted in the trophectoderm following vitrification and persisted in the day 14 elongated embryo stage.

 

  1. 13C-assisted metabolic flux analysis (13C MFA) is a powerful methodology to quantify intracellular fluxes through primary and intermediary metabolism. We have designed novel labeling experiments to obtain a metabolic flux map for this complex system. The methods developed by us demonstrate the extension of the MFA framework to complex mammalian systems.

 

  1. In the pig, the patterns of protein-to-protein interactions varied throughout follicle development. Proteins such as serine protease inhibitor, clade E (SERPINE); plasminogen activator, urokinase (PLAU); and plasminogen activator, urokinase receptor (PLAUR) appeared stage-specific. The “complement and coagulation cascades” was the common major pathway. In the mare, fifty-two proteins were common to all seasons. A total of 13 proteins were unique to either season, and 25 were shared between two seasons or more.
  2. Magnetic nanoselection permits high throughput targeting and removal of damaged spermatozoa from semen doses, leading to rapid and effortless enrichment of semen doses with highly motile, viable, and fertile spermatozoa for artificial insemination.
  3. The present study utilized the CRISPR/Cas9 genomic engineering to knockout pig conceptus PTGS2 gene expression. The current results with PTGS2-/- conceptuses and our previous work with CYP19A1-/- conceptuses suggests that both PGE2 and estrogens act as maternal recognition signals, which in the absence of the other can establish pregnancy beyond 17 days. However, conceptus production of estrogen is essential for successful maintenance of pregnancy beyond day 30 as both conceptus and endometrial PGs are not successful in compensating for lost estrogen production at this time.

 

  1. Semen from GnRHR-II KD boars had reduced sperm kinematics on Day 0, whereas littermate control sperm contained more proximal droplets. On Day 6, transgenic sperm kinetics were decreased, whereas littermate control sperm were slower. Treatment with a GnRHR antagonist (SB-75; 10 µM) decreased total motility of sperm, the percentage of morphologically normal sperm cells, and also impacted sperm kinematics.

 

  1. Testicular tissue from GnRHR-II KD males (n = 5) produced less testosterone per gram of testicular tissue than littermate controls. At maturity, GnRHR-II KD boars had reduced penis (P < 0.05) and prostate (P < 0.10) weights than control animals. However, protein amounts for both CYP11A1 and CYP17A1 were higher in testicular tissue from GnRHR-II KD vs. control animals. Neither volume or percentage of organelles for the nucleus, cytoplasm, mitochondria, lipid and late lysosomal bodies in Leydig cells differed between GnRHR-II KD vs. control testes.

 

  1. Design of a practical system to deliver a defined photostimulation (wavelength and intensity) protocol to cells/embryos in culture.
  1. The metabolomic profile analysis of bovine IVF and SCNT embryos through Raman spectroscopy was able to identify metabolomic differences between embryos of different developmental potential and embryos produced by IVF and SCNT.

 

  1. Apoptosis as a surrogate marker for nuclear reprogramming and embryo survival post-transfer A non-invasive marker for apoptosis distinguished between high quality and low quality embryos in vitro, but not in vivo.  There were distinct and consistent differences in gene expression and DNA methylation between highly apoptotic and lowly apoptotic embryos, which may be of interest. 

 

Objective 2: Refine methods to produce animals by genetic engineering or genome editing for the improvement of livestock production efficiency and development of human biomedical models.

 

  1. Optimized electroporation conditions for pig zygote CRISPR/Cas9 delivery. Design of guide RNAs effective at deleting the target DNA has been achieved.

 

  1. Established bovine ESCs from various embryo sources in alternative culture conditions.

 

  1. Targeting shRNA constructs for KDM1A were designed, tested in vitro, and currently used to study their function in early trophectoderm development in vivo. A multiplex PCR genotyping assay has been developed to distinguish XX and XY cells/tissues in sheep.

 

  1. Using in vitro assays, ARID3B was identified as a downstream target of LIN28-Let7 and a critical transcription factor complex and regulator of key genes necessary for placental development. LIN28B was identified as a regulator of androgen receptor in immortalized trophoblast cells through the action of Let7c.

 

  1. LIN41 and WNT pathway are downstream targets of the NANOG and LIN28 expression in reprogramming. Modulation of the LIN41 expression and WNT signaling can greatly enhance the human iPSC reprogramming efficiency. An additional downstream target of NANOG and LIN28 is FOXH1. Proper stimulation of FOXH1 expression in reprogramming is another mechanism behind NL-stimulated reprogramming efficiency.

 

  1. Bovine iPSCs were induced and the transcriptome profiles of the induced bovine cells and their human iPSC counterparts were generated. Allowing comparison of the reprogramming status of the bovine cells with human iPSCs.

 

  1. Naked DNA has been shown to bind naturally to the sperm, a method called spermmediated gene transfer (SMGT). In order to determine if the liposome-DNA complex would bind to sperm, real time PCR was used to detect GFP DNA and images of the sperm were analyzed using the Spatial Light Interference Microscopy (SLIM). SLIM confirmed the presence of liposomes on the sperm head and tail.

 

  1. Incorporation of the PLA reinforcing frame does not negatively influence the osteoinductive nature of the mineralized collagen scaffold. These findings suggest a strategy to address often competing bioactivity, mechanical strength, and shape-fitting design requirements for biomaterials for craniomaxillofacial bone regeneration.

 

  1. Using color Doppler ultrasound imaging that the highest pregnancy rate was observed in recipients with CLs with median Doppler blood flow, a palpable CL papillae, and no CL lacunae. A larger sample size is required to determine the accuracy of this measurement. Recipients with high to low CL blood flow are suitable for IVP embryo transfer programs. Alone, color Doppler imaging of the CL does not predict pregnancy rate.

 

  1. Bovine Leukosis Virus (BLV) in embryos produced by IVF with semen from BLV seropositive bulls could not be detected. Further, no viral presence was observed in the semen or media samples after sperm selection. In conclusion, these data indicate the use of BLV seropositive bull semen for the production of in vitro fertilized embryos posed no statistically significant risk of transmitting BLV to the embryos.

 

  1. The world’s first cria produced from a cryopreserved embryo was produced.

 

  1. The effect of the commonly used animal derived biological semen extender supplements, egg yolk and milk on the ability to induce the acrosome reaction following capacitation treatment commonly used in in vitro fertilization was studied. The semen cryopreserved with an extender supplemented with milk led to a significantly lower response to acrosome inducting agents than when semen was cryopreserved with an extender supplemented with egg yolk.

 

  1. An extended culture (6 hours) after vitrification-warming improved meiotic spindle recovery in bovine oocytes. Vitrified-warmed oocytes exposed to the extended culture had significantly more normal microtubular distribution. 

 

  1. The formulated liposomes can interact with boar spermatozoa, improve the proportions of motile and progressive spermatozoa, while decreasing the proportions of abnormal spermatozoa.

 

  1. Successful in using the CRISPR/Cas 9 gene editing system to knockout expression of specific conceptus genes. Conceptus genes for IL1B2, CYP19A1 and PTGS2 have been knockout using CRISPR/Cas 9 with fibroblastic cloning in pigs.

 

  1. Uncovered a previously unrecognized metabolic system that can help to maintain liver homeostasis under conditions of oxidative or toxic stress. The system uses catabolism of the essential sulfur amino acid methionine (Met) to generate cytosolic disulfide reducing power in the liver. This is the only NADPH-independent source of cytosolic disulfide reducing power that has, as yet, been characterized. This system consumes, in addition to Met, serine, and has broad impacts on diverse other metabolic pathways, including energetic pathways, the citric acid cycle, the folate cycle, polyamine biosynthesis pathways, methyltransferase reactions, and others.
  2. Developed an efficient protocol for the introduction of human specific mutations into the sheep fetal fibroblast cells using CRISPR/Cas9 RNP approach. Successfully introduced the F508del and G542X mutations in CFTR gene in SFFs. Generated biallelic NANOS2 knockout goats that served as recipients for exogenous spermatogonial stem cell transplantation.

 

  1. Published efficiency of CRISPR/Cas9 system to introduce targeted modifications in bovine is 10-50%. Our data suggests that 100% targeting could be achieved in bovine.

Impacts

  1. IMPACTS: 1. Endogenous testicular cortisol in the juvenile interval may restrict postpuberal sperm production in the boar. Regulation of in vivo Sertoli cell proliferation did not benefit from studies with in vitro monocultures. Our data suggest reduction of endogenous estrogen will not be an effective manipulation to increase bovine sperm production. 2. New roles of FSH in the development of preantral follicles are being discovered and will be critical for the development of culture systems that promote oogenesis in vitro. 3. Understanding bovine PGC biology represents a useful resource for in-vitro differentiation of bovine pluripotent stem cells to PGCs. 4. The epigenetic profiles of the bovine embryos is the fourth species ever characterized. Similarities and differences among different mammalian species were identified. The data serve as reference base for all bovine embryos produced by assisted reproductive technologies. 5. The results filled the knowledge gap on gene dosage compensation in a new species, the domestic sheep, an understudied species in developmental epigenetics. 6. Our data demonstrated the cilostamide during IVPM delayed meiotic progression in bovine. The specific inhibitors of cGMP do not affect the lipid profiles of the resulting oocytes and embryos after IVF. 7. Determining regulatory gene expression networks driving embryo development in vivo and in vitro as well as development potentially disrupted by cryopreservation will reveal targets for improving developmental competence in embryos produced by assisted reproductive technologies such as in vitro embryo production and somatic cell nuclear transfer. 8. Unraveling the metabolic basis of normal early embryo development will provide significant benefits to human and animal reproductive health. Improved embryonic competency following in vitro production and cryopreservation would stimulate the industry by lowering costs, especially those related to recipient management. 9. This study provides extensive and functional analyses of the pFF proteome changes during folliculogenesis and offers the potential for novel biomarker discovery in pFF for oocyte quality assessment. 10. The balance between the FF contents in prothrombin, plasminogen, and coagulation factor XII proteins favoring FF fluidity may be crucial at the peak of the ovulatory season (SUM), which peak may explain the reported lower incidence of hemorrhagic anovulatory follicles during the SUM season. 11. Magnetic nanoselection allowing specific removal of abnormal spermatozoa from semen samples appears as a promising tool for improving fertility of males, particularly during periods, such as heat stress during the summer months. 12. Utilization of the CRISPR/Cas 9 gene editing system provides a powerful tool to evaluate the role of genes during early conceptus development. Research has changed the paradigm of the role of IL1B2, estrogen and PTGS2 in maternal recognition of pregnancy and maintenance of pregnancy in pigs. 13. Acquired a change in fundamental knowledge regarding LH independent steroidogenesis and postnatal testicular development, implicating GnRH-II and its receptor in this important biological mechanism. We expect to better understand how the GnRH-II receptor is regulated within extra-pituitary tissues related to reproduction. 14. New pharmacological agents may be developed to manipulate the reproductive axis, leading to enhanced fertility rates and reduced incidences of endocrine disorders impacting reproduction. Data from these experiments could lead to novel swine-specific contraceptive methods to manage feral pig populations. 15. Identification of subfertile boars at a younger age would allow producers to focus resources on reproductively superior animals and market subfertile males prior to sexual maturity, significantly increasing their value. Improved identification of fertile vs. subfertile boars would contribute more doses of semen sold per ejaculate, reducing the cost of production. 16. Novel factors may be determined to improve semen extenders in swine, extending the lifespan of sperm and decreasing the cost of semen doses. Demonstration that GnRH-II and its receptor are important to sperm function, representing a potential avenue to improve semen preservation. 17. There was an immediate impact on the undergraduate research education of ten students involved in the development and execution of the projects. We reached the scientific community, professionals, and companies involved in bovine in vitro productions systems through the publication of our results in a scientific abstract 18. Delivery of CRISPR/Cas9 by electroporation will facilitate creation of gene-edited pigs 19. Efficient production of bESC from SCNT embryos enables the generation of pluripotent stem cell lines from valuable and well characterized individuals. 20. The long-term proliferation potential of bESCs make them good candidates for introducing complex and/or multiple gene edits in high genetic merit animals for advancing genetic improvement and/or enhancing production and biotechnological traits in cattle. 21. Understanding the role of LIN28A and LIN28B in sheep placenta will improve our understanding of the genetic regulation of placenta development and result in methods to improve somatic cell nuclear transfer in ruminants. 22. Similarly establishing that androgen and estrogen signaling through their receptors are regulated by KDM1A in the placenta, and are involved in placental angiogenesis in agriculture animals will lead to new insight into factors involved in early pregnancy losses. This also can lead to development of novel strategies and/or tools to identify or prevent early embryo mortality. 23. Generated new knowledge and database to elucidate the mechanisms for enhancement of reprogramming efficiency and pluripotency establishment which are key to the successful generation of bona fide biPSCs. 24. SLIM microscopy confirmed the presence of liposomes on the sperm head and tail. 25. A PLA reinforcing frame does not negatively influence the osteoinductive nature of a mineralized collagen scaffold. 26. Alone, color Doppler imaging of the CL does not predict pregnancy rate. The use of BLV seropositive bull semen for the production of in vitro fertilized embryos posed no statistically significant risk of transmitting BLV to the embryos. 27. The successful production of live offspring from a cryopreserved alpaca embryo opens the opportunity for germplasm preservation and transport for this species. 28. The demonstration that different semen extenders used during cryopreservation can effect the way spermatozoa respond to common capacitation treatments used in in vitro embryo production highlights the importance of developing in vitro methods applicable across a wide spectrum of conditions. 29. Disruption of the meiotic spindle has been a major component of the reduced developmental potential after oocyte cryopreservation. The ability to enhance spindle recovery post vitrification-warming is major step towards improving this capacity. 30. The newly designed liposomes were effective for simple interactions with sperm plasma membrane and molecule delivery, without impairing sperm motility. Further studies are ongoing with the replacement of doxorubicin with a DNA fragment. 31. Uncovered previously unrecognized systems that support survival and function of liver cells from a redox metabolism standpoint, which, in turn, provided insights into better therapies for treating some liver diseases or toxicities. From more of a bioengineering standpoint, these studies are demonstrating the power of being able to genetically manipulate hepatic metabolism in adult animals. This promises to provide interesting new ways to genetically modify nutrient use or intermediary metabolism of animals that could improve food-production characteristics of livestock without generating genetically modified food products. 32. Our results should assist in utilizing the technology in introducing targeted modifications in bovine. Our publication indicates that unintended mutations can be introduced by CRISPR/Cas9 system, if not carefully designed. The study suggests that careful consideration should be made prior to apply the technology in genetically engineered animal production.

Publications

PUBLICATIONS:

 

Refereed journal articles

Berger, T. 2019. Testicular estradiol and the pattern of Sertoli cell proliferation in prepuberal bulls. Theriogenology 136:60-65. doi: 10.1016/j.theriogenology.2019.06.031

 

Berger, T., P. Sidhu, S. Tang, and H. Kucera. 2019. Are testicular cortisol and WISP2 involved in estrogen-regulated Sertoli cell proliferation? Animal reproduction science 207:44-51. doi: 10.1016/j.anireprosci.2019.05.014

 

Kucera, H., B. Puschner, A. Conley, and T. Berger. 2019. Tissue steroid levels in response to reduced testicular estrogen synthesis in the male pig, Sus scrofa. PloS one 14(4):e0215390. doi: 10.1371/journal.pone.0215390

 

Candelaria, J., and A. Denicol. 2020 (accepted). Characterization of isolated bovine preantral follicles based on morphology, diameter and cell number. Zygote. doi: 10.1017/S0967199419000832

 

Goszczynski DE, Denicol AC, Ross PJ. 2019. Gametes from stem cells: Status and applications in animal reproduction. Reprod Domest Anim. 54 Suppl 4:22–31. doi:10.1111/rda.13503

 

Bogliotti YS, Chung N, Paulson E, Chitwood J, Halstead M, Kern C, Schultz RM, Ross PJ. Transcript profiling of bovine embryos implicates specific transcription factors in the maternal-to-embryo transition. Biology of Reproduction, 2019 Nov 11. pii: ioz209. doi: 10.1093/biolre/ioz209. [Epub ahead of print]

 

Parons Aubone AM, Bisiau CM, McCue PM, Bouma GJ. 2019. Presence of Clock genes in equine full-term placenta. J Anim Sci Submitted.

 

Ali A, Anthony RV, Bouma GJ, Winger QA. 2019. LIN28-let-7 axis regulates genes in immortalized human trophpblast cells by targeting the ARID3B-complex. FASEB J. Nov;33(11):12348-12363.

 

West RC, Russ JE, Bouma GJ, Winger QA. 2019. BRCA1 regulates HMGA2 levels in the Swan71 trophoblast cell line. Mol Reprod Dev Nov;86(11):1663-1670.

 

McWhorter ES, West RC, Russ JE, Winger QA, Bouma GJ. 2019. LIN28B regulates androgen receptor (AR) in human trophoblast cells. Mol Reprod Dev. Sep;86(9):1086-1093.

 

West RC, McWhorter ES, Ali A, Goetzman LN, Russ JE, Anthony RV, Bouma GJ, Winger QA. 2019. HMGA2 is regulated by LIN28 and BRCA1 in human placental cells. Biol Repod Jan 1;100(1):227-238.

 

An, L., S. L. Marjani, Z. Wang, Z. Liu, R. Liu, F. Xue, J. Xu, T. L. Nedambale, L. Yang, X. C. Tian, L. Su, and F. Du. 2019. Magnesium is a critical element for competent development of bovine embryos. Theriogenology 140:109-116. doi: 10.1016/j.theriogenology.2019.08.015

 

Duan, J., L. Zhu, H. Dong, X. Zheng, Z. Jiang, J. Chen, and X. C. Tian. 2019. Analysis of mRNA abundance for histone variants, histone- and DNA-modifiers in bovine in vivo and in vitro oocytes and embryos. Sci Rep 9(1):1217. doi: 10.1038/s41598-018-38083-4

 

Duan, J. E., K. Flock, N. Jue, M. Zhang, A. Jones, S. A. Seesi, I. Mandoiu, S. Pillai, M. Hoffman, R. O'Neill, S. Zinn, K. Govoni, S. Reed, H. Jiang, Z. C. Jiang, and X. C. Tian. 2019. Dosage Compensation and Gene Expression of the X Chromosome in Sheep. G3 (Bethesda) 9(1):305-314. doi: 10.1534/g3.118.200815

 

Duan, J. E., Z. C. Jiang, F. Alqahtani, I. Mandoiu, H. Dong, X. Zheng, S. L. Marjani, J. Chen, and X. C. Tian. 2019. Methylome Dynamics of Bovine Gametes and in vivo Early Embryos. Front Genet 10:512. doi: 10.3389/fgene.2019.00512

 

Duan, J. E., W. Shi, N. K. Jue, Z. Jiang, L. Kuo, R. O'Neill, E. Wolf, H. Dong, X. Zheng, J. Chen, and X. C. Tian. 2019. Dosage Compensation of the X Chromosomes in Bovine Germline, Early Embryos, and Somatic Tissues. Genome Biol Evol 11(1):242-252. doi: 10.1093/gbe/evy270

 

Tian, XC. 2019. The past, present and future of bovine pluripotent stem cells – a brief overview. Frontiers of Agricultural Science and Engineering 6(1): 3-7. https://doi.org/10.15302/J-FASE-2018247

 

Wang L, Su Y, Huang C, Yin Y, Zhu J, Knupp A, Chu A, Tang Y.

FOXH1 Is Regulated by NANOG and LIN28 for Early-stage Reprogramming.

Scientific Reports. 2019. 9:16443 | https://doi.org/10.1038/s41598-019-52861-8.

 

Wang L, Su Y, Huang C, Yin Y, Chu A, Knupp A, Tang Y.

NANOG and LIN28 Dramatically Improve Human Cell Reprogramming by Modulating LIN41 and Canonical WNT Activities. Biol. Open. 2019. 8: bio047225 doi: 10.1242/bio.047225.

 

Rubessa, M., Lotti, S.N., Kandel, M.E., Popescu, G., Wheeler, M.B. 2019. SLIM

Microscopy allows for visualization of DNA-containing liposomes designed for spermmediated

gene transfer in cattle. Mol Biol Rep. 2019 Feb;46(1):695-703. doi:

10.1007/s11033-018-4525-9. doi: 10.1007/s11033-018-4525-9. [Epub ahead of print]

 

Correia, H.H.V., Vieira, L.A., Mielgo, C.M., Paes, V.M., Alves, B.G., Viana, J.R.,

Wheeler, M.B., Rodrigues, A.P.R., Figueiredo, J.R. 2019. Cilostamide affects in a

concentration and exposure time-dependent manner the viability and the kinetics of in vitro

maturation of caprine and bovine oocytes. Research in Veterinary Science 2018 Nov

12;122:22-28. doi: 10.1016/j.rvsc.2018.11.002. [Epub ahead of print] Research in Veterinary

Science 122 (2019) 22–28.

 

Dewey, M.J., Johnson, E.M., Weisgerber, D.W., Wheeler, M.B., Harley, B.A.C. 2019. Shapefitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells. Journal of the Mechanical Behavior of Biomedical Materials 95:21–33.

DOI: 10.1016/j.jmbbm.2019.03.017

 

Bertels, J., Rubessa, M., Kandel, M.E., Bane, T., Milner, D.J., Popescu, G., and Wheeler,

M.B. 2019. Zinc’s Effect on the Differentiation of Porcine Adipose-Derived Stem Cells into

Osteoblasts. Journal of Regenerative Medicine 8:2-1-7.

 

Stewart, J., Rubessa, M., Polkoff, K.M., Lotti, S., Wheeler, M.B. 2019. Risk of transmission

of Bovine Leukosis Virus (BLV) in abattoir-derived in vitro produced embryos. International

Journal of New Technology and Research (IJNTR) ISSN:2454-4116, Volume-5, Issue-9,

September 2019 Pages 01-06. https://doi.org/10.31871/IJNTR.5.9.5

 

Rubessa, M., Kandel, M.E., Schreiber, S., Meyers, S., Beck, D.H., Popescu, G., and

Wheeler, M.B. 2019. Sperm selection methods for bovine in vitro embryo production,

characterized by spatial light interference microscopy. Systems Biology in Reproductive

Medicine (accepted).

 

Kandel, M.E., Rubessa, M., Meyers, S., Szewczyk, M.J., Yu, F., Wheeler, M.B., Popescu, G.

(2019). High sensitivity SLIM imaging to correlate sperm morphology and fertility outcomes.

Proc.SPIE 10887, Quantative Phase Imaging V, 108871B,

https://doi.org/10.1117/12.2513238.

 

 

Belala R, L Briand-Amirat, A Martinot, C Thorin, S Michaud, S Desherces, CR Youngs, and D Bencharif. 2019. A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa. Reprod. Dom. Anim. 54(8):1131-1138 https://doi.org/10.1111/rda.13476.

 

Elshazly AG and CR Youngs. 2019. Feasibility of utilizing advanced reproductive technologies for sheep breeding in Egypt. Part 1. Genetic and nutritional resources. Egyptian Journal of Sheep & Goat Sciences 14(1):39-52. https://ejsgs.journals.ekb.eg/article_33235_c2fd1cc6b968bbc2124356222fc929cf.pdf

 

Gómez-Quispe OE, GA Gutierrez-Reynoso, A Gallegos-Cardenas, FG Fumuso, M Asparrin, M Asparrin-Del Carpio, CW Jara, D Salazar-Ponce, M Miguel-Gonzales, CR Youngs, HW Vivanco. 2019. Motility and recovery of alpaca (Vicugna pacos) spermatozoa after centrifugation in a density gradient solution. Iranian Journal of Veterinary Research 20(2):96-104. http://ijvr.shirazu.ac.ir/article_5257_80ac7e09a2bbc52a890720d283c01227.pdf

 

Long JA, HD Blackburn, A Martin, FG Silversides, RL Taylor, Jr, and CR Youngs.  2019. Protecting Food Animal Gene Pools for future generations – a paper in the series on The Need for Agricultural Innovation to Sustainably Feed the World by 2050. Council on Agricultural Science and Technology (CAST) Issue Paper 65, 24 pages.

 

West R.C., Hao Ming*, Deirdre M. Logsdon*, Jiangwen Sun, Sandeep K. Rajput, Rebecca A. Kile, William B. Schoolcraft, R. Michael Roberts, Rebecca L. Krisher, Zongliang

Jiang#, Ye Yuan#. Dynamics of trophoblast differentiation in peri-implantation stage human embryos. PNAS: Proc Natl Acad Sci U S A. 2019 Oct 21. PMID: 31636193.

 

Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, Scott R, Horvath T, Seli E. Mitofusin 1 is required for female fertility and to maintain ovarian follicular reserve. Cell Death & Disease, 2019 Jul 22;10(8):560. PMID: 31332167.

 

Zhang M, Bener MB, Jiang Z, Wang T, Esencan E, Scott R, Horvath T, Seli E. Mitofusin 2 plays a role in oocyte and follicle development, and is required to maintain ovarian follicular reserve during reproductive aging. Aging, 2019 Jun 16;11(12):3919-3938. PMID: 31204316.

 

Paes, V. M., Liao, S., Figueiredo, J. R., Willard, S. T., Ryan, P. L., Feugang, J.-M. (2019). Proteome changes of porcine follicular fluid during follicle development. Journal of animal science and biotechnology, 10, 94.

 

Dutra, G. A., Ishak, G. M., Pechanova, O., Pechan, T., Peterson, D. G., Jacob JCF, Willard, S. T., Ryan, P. L., Gastal, E. L., Feugang, J.-M. (2019). Seasonal variation in equine follicular fluid proteome. Reproductive biology and endocrinology: RB&E, 17(1), 29.

 

Durfey, C. L., Swistek, S. E., Liao, S., Crenshaw, M. A., Clemente, H. J., Thirumalai RVKG, Steadman, C. S., Ryan, P. L., Willard, S. T., Feugang, J.-M. (2019). Nanotechnology-based approach for safer enrichment of semen with best spermatozoa. Journal of animal science and biotechnology, 10, 14.

 

Geisert R.D., P. Sutvosky, M.C. Lucy, F.F. Bartol and A.E. Meyer. 2019. Chapter 15: Reproductive Physiology of Swine. In: Animal Agriculture: Sustainability, Challenges and Innovations.  Eds. F.W. Bazer, G.C. Lamb and G. Wu. Academic Press, Elsevier Inc.

Pfeiffer C.A., A.E.Meyer, K.E. Brooks, P.R. Chen, J. Milano-Foster, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, L.A. Ciernia, M.F. Smith, K.D. Wells, T.E. Spencer, R.S. Prather, and R.D. Geisert. 2019. Ablation of conceptus PTGS2 provides a new understanding of early pregnancy events in the pig. Biol Reprod  doi.org/10.1093/biolre/ioz192.

Meyer A.E., C.A. Pfeiffer, K.E. Brooks, L.D. Spate, J.A. Benne, R.Cecil, M.S. Samuel, C.N. Murphy, S. Behura, M.K. McLean, L.A. Ciernia, M.F. Smith, K.M. Whitworth, K.D. Wells, T.E. Spencer, R.S. Prather and R.D. Geisert. 2019. New perspective on conceptus estrogens in maternal recognition and pregnancy establishment in the pig. Biol Reprod 101: 148–161. https://doi.org/10.1093/biolre/ioz058

 

Dagnell, M., E. E. Schmidt, and E. S. J. Arner. 2018. The A to Z of modulated cell patterning by mammalian thioredoxin reductases. Free Radic Biol Med 115:484-496. doi: 10.1016/j.freeradbiomed.2017.12.029

 

Doka, E., T. Ida, M. Dagnell, Y. Abiko, N. L. Cong, N. Balog, T. Takata, B. Espinosa, A. Nishimura, Q. Cheng, Y. Funato, H. Miki, J. Fukuto, J. R. Prigge, E. E. Schmidt, E. S. J. Arnér, Y. Kumagai, T. Akaike, and P. Nagy. 2020. Control of protein function through oxidation and reduction of persulfidated states. Science Advances (in press)

 

McLoughlin, M. R., J. R. Prigge, D. J. Orlicky, P. Krishna, E. A. Talago, I. R. Cavigli, S. Eriksson, C. G. Miller, J. A. Kundert, V. I. Sayin, R. A. Sabol, J. Heinemann, L. O. Brandenberger, S. V. Iverson, B. Bothner, T. Papagiannakopoulos, C. T. Shearn, E. S. J. Arnér, and E. E. Schmidt. 2019. TrxR1, Gsr, and oxidative stress determine hepatocellular carcinoma malignancy. Proc Natl Acad Sci U S A. 116, 11408-11417.

 

Miller, C. G., A. Holmgren, E. S. J. Arner, and E. E. Schmidt. 2018. NADPH-dependent and -independent disulfide reductase systems. Free Radic Biol Med 127:248-261. doi: 10.1016/j.freeradbiomed.2018.03.051

 

Miller, C. G., and E. E. Schmidt. 2019. Disulfide reductase systems in liver. Br J Pharmacol 176(4):532-543. doi: 10.1111/bph.14498

 

Roggenbeck, B. A., E. M. Leslie, S. T. Walk, and E. E. Schmidt. 2019. Redox metabolism of ingested arsenic: Integrated activities of microbiome and host on toxicological outcomes. Curr Opin Toxicol., 13, 90-98.

 

Desaulniers, A.T., R.A. Cederberg, E.P. Carreiro, C.B. Gurumurthy and B.R. White. 2018. A transgenic pig model expressing a ZsGreen1 reporter across an extensive array of tissues. Anim. Biotechnol. (Under Review).

 

Gruhot, T.R., L.A. Rempel, B.R. White and B.E. Mote. 2019. The effect of varicocele on semen quality in boars exposed to heat stress. Translational Anim. Sci. [Epub ahead of print].

 

Fan, Z., M. Yang, M. Regouski, and I.A. Polejaeva, Gene Knockouts in Goats Using CRISPR/ Cas9 System and Somatic Cell Nuclear Transfer. Methods Mol Biol, 2019. 1874: p. 373-390.

 

Gash, K.K., M. Yang, Z. Fan, M. Regouski, H.M. Rutigliano, and I.A. Polejaeva, Assessment of microchimerism following somatic cell nuclear transfer and natural pregnancies in goats. J Anim Sci, 2019. 97(9): p. 3786-3794. PMCID: PMC6735858.

 

Regouski, M., O. Galenko, J. Doleac, A.L. Olsen, V. Jacobs, D. Liechty, K.L. White, T.J. Bunch, P.M. Lee, H.M. Rutigliano, I.A. Polejaeva, and M.J. Cutler, Spontaneous Atrial Fibrillation in Transgenic Goats With TGF (Transforming Growth Factor)-beta1 Induced Atrial Myopathy With Endurance Exercise. Circ Arrhythm Electrophysiol, 2019. 12(11): p. e007499.

 

Wu, H., Z. Fan, M. Brandsrud, Q. Meng, M. Bobbitt, M. Regouski, R. Stott, A. Sweat, J. Crabtree, R.J. Hogan, R.A. Tripp, Z. Wang, I.A. Polejaeva, and E.J. Sullivan, Generation of H7N9-specific human polyclonal antibodies from a transchromosomic goat (caprine) system. Sci Rep, 2019. 9(1): p. 366. PMCID: PMC6344498.

 

Carey K, Ryu J, Uh K, Lengi AJ, Clark-Deener S, Corl BA, Lee K. 2019. Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos. BMC Biotechnol. May 6;19(1):25. doi: 10.1186/s12896-019-0517-7.

 

Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

 

Berger, T., H. Kucera, A. Conley, and B. Puschner. 2019. Steroid concentrations in boar tissues. https://doi.org/10.25338/B85P5T

 

Elizabeth Johnson. 2019. Effects of intramuscularly injected plant-derived antimicrobials in the mouse model. MS thesis. University of Connecticut.

 

Characterizing cytoplasmic maturation and improving competence of bovine in vitro matured oocytes.  Louisiana State University Ph D Dissertation.  August 2018.

 

Effect of heat stress on the epigenetic profile of bovine oocytes and embryos.  Louisiana State University Ph D Dissertation.  December 2018.

 

Chung, J., Sriram, G., and C. Keefer.  (submitted) Magnetic Nanoparticle Technology Improves In-vitro Attachment of Cattle (Bos taurus) Trophectoderm Cells. Biotechnology Letters

 

Pfeiffer CA. 2019.  Ablation of Conceptus PTGS2 Provides a New Understanding of Early Pregnancy Events in the Pig.  Master of Science Thesis.

 

Alhojaily, Sameer M. (2019). The Effect of Lactation and Energy Status on Gene Expression in the Main Reproductive Tissues of Lactating Dairy Cattle. PhD Dissertation  #7588.  Utah State University.  https://digitalcommons.usu.edu/etd/7588

 

Moley, Laura A., (2019).  Epigenetic Reprogramming, Apoptosis, and Developmental Competence in Cloned Embryos.  PhD Dissertation #7571.  Utah State University.  https://digitalcommons.usu.edu/etd/7571

 

Lee K, Farrell K, Uh K. 2019. Application of genome editing systems to enhance available pig resources for agriculture and biomedicine. Reproduction, Fertility and Development 32(2) 40-49 https://doi.org/10.1071/RD19273

 

Abstracts:

Candelaria, J., B. Rabaglino, and A. Denicol. 2020. Transcriptomic changes in bovine ovarian cortex in response to FSH signaling. 46th Annual Meeting of the IETS. New York, NY.

 

Denicol, A., B. Weldon, and L. Aguiar. 2020. Preliminary characterization of ovarian stem cells from bovine ovaries. 46th Annual Meeting of the IETS. New York, NY.

 

Soto DA and Ross PJ. Single-Cell RNA Sequencing Reveals Similarities Between Bovine and Human Primordial Germ Cell Development. 52nd Annual Conference of the Society for the Study of Reproduction, San Jose, CA July 18-21, 2019

 

Ross PJ. Transcriptional and Epigenetic Reprogramming During Bovine Preimplantation Development. 52nd Annual Conference of the Society for the Study of Reproduction, San Jose, CA July 18-21, 2019

 

Paulson EE, Ross PJ, Schultz RM. Expression Pattern And Role Of miRNAs During Early Development In The Cow. 52nd Annual Conference of the Society for the Study of Reproduction, San Jose, CA July 18-21, 2019

 

Park I, Kim SK, Ross PJ. Neurogenin 3 Disruption By CRISPR/Cas9 Electroporation Of Porcine Zygotes. 52nd Annual Conference of the Society for the Study of Reproduction, San Jose, CA July 18-21, 2019

 

Navarro M, Rodrigues Sangalli J. Ross PJ. Derivation and characterization of bovine embryonic stem cells from different embryo sources. Transgenic Animal Research Conference XI! (TARC XI!): Tahoe City, CA, 2019.

 

Gerrit J. Bouma, Asghar Ali, Erin S. McWhorter, Rachel C. West, Quinton A. Winger. Histone lysine demethylase function in placental trophoblast cells. 2019 Perinatal Biology Symposium, Aspen-Snowmass, CO, USA.

 

Asghar Ali, Russell V Anthony, Gerrit J. Bouma, Quinton A. Winger. LIN28-let-7 axis regulates gene expression in ovine placenta in vivo. Society for the Study of Reproduction 52nd Annual Meeting, San Jose, CA, USA.

 

Asghar Ali, Gina C Nay, Russell V Anthony, Gerrit J Bouma,  Quinton A Winger. Genes Governing Placental Development are Regulated by ARID3A-ARID3B-KDM4C Complex. 2019 Society for Reproductive Investigation 66thAnnual Meeting, Paris, France.

 

Thomas R. Hansen, Hana Van Campen, Jeanette V. Bishop, Gerrit J. Bouma, Quinton A. Winger, Leticia D.P. Sinedino, Christie E. Mayo, Richard A. Bowen. Maternal influenza A virus infection decreases the expression of T cell differentiation genes in the murine fetal thymus. Society for the Study of Reproduction 52nd Annual Meeting, San Jose, CA, USA.

 

Duan JE, Jiang Z, Alqahtani F, Mandoiu I, Dong H, Zheng X, Marjani SL, Chen J, and Tian XC. Whole genome bisulfite sequencing of bovine gametes and in vivo produced preimplantation embryos. Reproduction, Fertility and Development 31(1) 126. Poster at the 45th annual meeting of the International Embryo Technology Society, January 20-23, 2019, New Orleans, USA.

 

Silva, E. P., M. K. Sermersheim, P. V. Marchioretto, R. Della Mea, L. M. Naves, I. Rivelli, F.

Rochelle, L. F. T. Nasser, M. Rubessa and M. B. Wheeler. 2019. A comparison of in vitro embryo production between heifers and lactating Holstein donors without superstimulation. Reproduction, Fertility and Development. 31(1): 195-195.

 

Siegel, L., T. Bane, J. Bertels, K. Ratz, M. Rubessa and M. Wheeler. 2019. Effect of selenium on the differentiation of porcine adipose-derived stem cells into osteoblasts. Reproduction, Fertility and Development. 31(1): 216-217.

 

Salerno, F., M. Rubessa, B. Gasparrini and M. Wheeler. 2019. Effect of deuterium oxide on bovine oocyte cryotolerance. Reproduction, Fertility and Development. 31(1): 140-140.

 

Padoveze, L. R., M. Rubessa, C. E. Ambrosio and M. B. Wheeler. 2019. The effects of different

concentrations of MgSO4 in osteogenic differentiation. Reproduction, Fertility and Development.

31(1): 217-218.

 

Bane, T., L. Siegel, J. Bertels, K. Ratz, M. Rubessa and M. Wheeler. 2019. The effect of copper on the differentiation of adipose-derived stem cells into osteoblasts. Reproduction, Fertility and

Development. 31(1): 229-230.

 

Vivanco-Mackie HW, MD Ponce-Salazar, M Miguel-Gonzales, CR Youngs, C. Jara, and M Asparrin.  2019. Comparative study between slow freezing and vitrification on the survival rate of cryopreserved alpaca embryos post-transfer. Reprod. Fertil. Dev. 31(1):182.

 

Gatenby, L. and KR Bondioli. 2019.  The effect of biological extenders on in vitro induction of the acrosome reaction in bovine spermatozoa.  Reprod. Fertil. Dev..

 

Gutierrez, EJ, Z. Jiang, KR Bondioli.  2019.  Extended culture after vitrification warming helps in spindle recovery of bovine oocytes.  Reprod. Fertil. Dev.

 

Jiang Z, Gutierrez E, Foster B, Ming H, Gatenby L, Mak C, Pinto C, Bondioli K. Effect of vitrification on global gene expression dynamics of bovine elongating embryos. Reprod. Fertil. Dev. 3 December 2019.

 

Ming, H.*, J. Sun, R. Pasquarielle, J. Herrick, Y. Yuan, E.J. Gutierrez, L. Gatenby, K. R. Bondioli, R.L. Krisher, Z Jiang#. The landscape of accessible chromatin in bovine oocytes and early embryos. Reprod. Fertil. Dev. 3 December 2019.

 

Rajput S, West R, Ming H*, Logsdon D, Kile R, Grimm C, Sun J, Schoolcraft W, Krisher R, Jiang Z#, Yuan Y#. Peri-implantation stage human embryos cease cell proliferation and increase metabolism to prioritize important cellular events for implantation. 52th SSR, San Jose, CA, 2019.

 

West R, Ming H*, Logsdon D, Kile R, Grimm C, Rajput S, Sun J, Schoolcraft W, Krisher R, Jiang Z, Yuan Y#. Single Cell RNA-Sequencing Reveals Critical Role of Interferon Signaling in Human Peri-Implantation Stage Embryos. 52th Annual conference of Society for the Study of Reproduction, San Jose, CA, 2019.

 

Yuan Y, Sun J, Ming H*, Logsdon D, Schoolcraft W, Krisher R, Jiang Z#. Single-cell whole genome bisulfite sequencing reveals DNA methylation dynamics of trophoblast differentiation in human peri-implantation stage embryos. Fertility Sterility. 2019, O-237.

 

Yuan Y, West R, Ming H*, Logsdon D, Kile R, Grimm C, Rajput S, Sun J, Schoolcraft W, Krisher R, Jiang Z#. Picturing the dynamics of trophoblast differentiation in per-implantation stage human embryos by single cell RNA sequencing. 52th SSR, San Jose, CA, 2019.

 

Zhang M, Bener M, Jiang Z, Wang T, Esencan E, Scott III R, Seli E. Loss of mitochondrial dynamics in oocytes results in infertility and diminished ovarian reserve. Fertility Sterility.

 2019, O-217.

 

Zhang M, Bener M, Jiang Z, Wang T, Esencan E, Scott III R, Seli E. Loss of mitochondrial fusion protein MFN2 results in telomere shortening, reduced fertility, and accelerated depletion of follicular pool. Fertility Sterility. 2019, P-237.

Chung, J., Clifford, R., Sriram, G., and C. Keefer. 2019. Flux analysis of aerobic glycolysis in bovine blastocysts and CT1 cells. Reprod. Fert. Dev. 31(1), 159.

 

Lugar, D., Adhikari, S., Keefer, C.L., and Sriram, G. 13C Metabolic Flux Analysis of Pre-Implantation Bovine Embryos: Surmounting Challenges Due to Small Sample Size and Complex Media, American Chemical Society National Meeting, March 22-26, 2020

Feugang, J.M., Eggert, M.W., Park, S.B., Popoola, M.A., Steadman, C.S., Arnold, R.R., Ryan, P.L. and Willard, S.T., 2019. 203 Newly designed liposome nanoparticles for drug delivery into boar spermatozoa. Reproduction, Fertility and Development, 31(1), pp.227-227.

Pfeiffer C.A., A.E. Meyer., L.D. Spate, J.A. Benne, R.F. Cecil, T.E. Spencer, R.S. Prather, and R.D. Geisert. 2019. Conceptus Prostaglandin Synthase 2 is Not Essential for Early Development and the Establishment of Pregnancy in the Pig. 52nd Annual Meeting of SSR, P432

 

Johns D.N., C.G. Lucas, C.A. Pfeiffer, L.D. Spate, J.A. Benne, R.F. Cecil, K.M. Wells, T.E. Spencer, R.S. Prather, and R.D. Geisert. 2019. Pig Conceptus Expression of Type I Interferon Delta and Type II Interferon Gamma During Early Pregnancy.  The 16th Annual Gilbert S. Greenwald Symposium on Reproduction and Regenerative Medicine, P25.

 

Ross, C.E., F.H. Choat, K.N. Plager, A.T. Desaulniers, R.A. Cederberg, G.A. Mills and B.R. White. 2019. Effect of GnRH-II and its receptor on boar semen quality. Reprod. Fertil. Dev. 32:201.

Ebrecht, M.A., C.E. Ross, R.A. Cederberg, K.W. Lovercamp, A.T. Desaulniers and B.R. White. 2019. Effects of GnRH-II on spermatogenic function of swine. Proceedings of the 16th Annual Gilbert S. Greenwald Symposium on Reproduction and Perinatal Research. p. 44.

Desaulniers, A.T., R.A. Cederberg, G.A. Mills and B.R. White. 2019. Use of a genetically-engineered swine line to elucidate the role of GnRH-II and its receptor in gilts. J. Anim. Sci. 97(Suppl. 2):122-123.

 

Checura, C. M., S. L. Pratt, L. V. Campbell, K. Farmer, G. Loughlin, M. Mitchell, A. Sandford, A. M. Treske, H. Malter. 2019. The use of photostimulation to enhance oocyte cytoplasmic maturation. Reprod. Fertil. Dev. 32(2): 227. (Abstr.) doi:10.1071/RDv32n2Ab197

 

Perisse I.V., Z. Fan, Y. Liu, M. Regouski, A. Van Wettere, Z. Wang, A. Harris, K. L. White and I. A. Polejaeva (2019). Towards the correction of meconium ileus with cystic fibrosis transmembrane conductance regulator (CFTR) intestinal expression in CFTR knockout sheep. Reproduction, Fertility and Development 31(1) 228-228 https://doi.org/10.1071/RDv31n1Ab205

 

Fan Z., M. Regouski, M. Brandsrud, H. Wu, Y. Liu, Z. Wang, E. Sullivan and I. A. Polejaeva (2019). Towards the generation of transchromosomic goats for the production of fully human immunoglobulin. Reproduction, Fertility and Development 31(1) 228-228 https://doi.org/10.1071/RDv31n1Ab206

 

Keim J., Y. Liu and I. A. Polejaeva (2019). Increasing in vitro embryonic development through improved oocyte maturation in cattle oocytes. Reproduction, Fertility and Development 31(1) 213-213 https://doi.org/10.1071/RDv31n1Ab177

 

Silva A.C., Morgado K.P., Davies C.J., Polejaeva I.A. and Rutigliano H.M. (2019) Gene expression profiles of immune cells under the influence of bovine trophoblast cell derived extracellular vesicles. The 52 annual meeting of the Society for the Study of Reproduction. July 18-21, 2019. San Jose, CA.

Cuthbert J., Russell S., Meng Q., Polejaeva I.A., White K., Benninghoff  A. (2019) Somatic cell nuclear transfer in early bovine embryo development is associated with changes in small non-coding RNA species. The 52 annual meeting of the Society for the Study of Reproduction. July 18-21, 2019. San Jose, CA.

Perisse I.V., Fan Z., Wang Z., Harris A., White K.L., Polejaeva I.A. (2019) Efficient introduction of G542X mutation in the CFTR gene in sheep fetal fibroblast using CRISPR/Cas9 ribonucleoprotein. Cystic Fibrosis Foundation Research Conference. June 23-26, 2019. Stowe, VT.

 

Moley L.M, Jones R., Kaundal R., Thomas A., Benninghoff A., Isom S.C. (2019)  Gene expression analysis and DNA methylation patterns of porcine SCNT blastocysts with high and low incidence of apoptosis.  Reproduction, Fertility and Development 31(1), 128-128.  https://doi.org/10.1071/RDv31n1Ab5

 

Farrell K, Uh K, Haines H, Lee K. 2019. Expression patterns of PRDM family genes in porcine preimplantation embryos. Reproduction, Fertility and Development 32(2)

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