Brief Summary of Minutes of Annual Meeting

NC 1194 Annual Report Accomplishments

10/01/2018 – 09/30/2019

Station: Arizona (University of Arizona)

PI: Dr. Jeong-Yeol Yoon

Investigators/Participants: Soo Chung (PhD student), Robin E. Sweeney (PhD student), Katherine E. Klug (PhD student), Tiffany-Heather Ulep (PhD student), Benjamin Alouidor (MS student), Vina Nguyen (MS student), Lane E. Breshears (undergraduate student), Trinny Tat (undergraduate student), Christian M. Jennings (undergraduate student), Elizabeth Budiman (undergraduate student), Sean Perea (undergraduate student), Alexander S. Day (undergraduate student), Katelyn Sosnowski (undergraduate student), Alexa Shumaker (undergraduate student), Brandon T. Nguyen (undergraduate student), Raymond K. Wong (Professor; Collaborator in Medical Pharmacology), Lingling An (Professor; Collaborator in Biosystems Engineering and Statistics), Walter Q. Betancourt (Professor; Collaborator in Soil, Water and Environmental Science), and Kelly A. Reynolds (Professor; Collaborator in Public Health).

Accomplishments:

There is a growing need to develop a handheld, smartphone-based biosensor that can detect the type and concentration of pathogens from myriads of food (fresh produce and meat) and water (waste and irrigation) samples, as well as urine, blood, and tissue samples from animal and human subjects. These biosensors must be designed and manufactured to be easy-to-use, all-in-one, and extremely sensitive (down to single cell level, single genomic copy level, or picogram protein level).

During the period from October 1^st, 2018 to September 30^th, 2019, we have published 7 journal papers. In addition, there are four journal papers and one text book accepted in this period (not listed here – will be reported in the next year’s report). Some of our work have received recognitions from professional societies, such as “Most Downloaded Journal Paper” from IEEE (listed #4 below) and “Highly Cited Journal Paper” from Royal Society of Chemistry (RSC) (Anal. Meth. 8: 6591-6601; published in late 2016 but the recognition was made in 2019). Most importantly, our work on single copy level detection of norovirus was selected one of only 17 papers (out of >12,000 papers) to be press-released by American Chemical Society (ACS) Fall 2019 National Meeting & Expo at San Diego Convention Center. There was also a press conference, broadcasted live on YouTube, and subsequently picked up by numerous news media, including NPR News from Washington Headquarter. Media coverage is summarized in #5 shown below.

We are also translating our handheld biosensor technology into real-world practice, through collaborating with Tucson Water and Korea Institute of Ocean Science and Technology (KIOST), who have also provided research grants to our lab.

Station: Hawaii (University of Hawaii)

PI: Daniel M. Jenkins

Investigators/Participants: Daniel M. Jenkins (Professor); Lena Diaz (PhD Student / Graduate Assistant)

Accomplishments:

Our work has focused on two main projects- the development of open-source hardware and software for a wireless potentiostat project to facilitate commercialization of nanotechnology-based sensors for field use, and development of molecular probes and classification algorithms for discrimination of single nucleotide polymorphisms (SNPs) in pathogenic organisms.

The potentiostat project, “ABE-Stat” was developed in collaboration with project participants at the Universities of Florida and Georgia, and has been fully characterized and documented in an invited open-access issue of the Journal of the Electrochemical Society. The device is the first open-source project of it’s kind with a fully wireless smart-phone interface, and to incorporate Electrochemical Impedance Spectroscopy (EIS) capability across a wide spectrum (0.1 Hz to 100 kHz). We have shared prototypes of the device with numerous researchers in the US and abroad.

A second iteration of the device was designed and assembled to improve signal to noise performance especially for dynamic voltammetry applications, and to address some issues in consistency of EIS measurements across the spectrum that resulted in discontinuities in recorded spectra in for non-linear systems using the original design. We are currently developing firmware updates to more fully characterize this system in anticipation of making this new design more widely available to the research community.

For our molecular probe design we incorporated mismatched ribonucleotides into quenched fluorescence DNA probes (i.e. strands terminated with fluorophore and quencher molecules), and observed changes in fluorescence due to differential probe annealing and RNAase actuated cleavage to different SNP variants of the target gene. While individual probes exhibited some cross reactivity with their non-target variants, characteristic patterns of the real-time reactions could reliably be used to predict which gene variant was present, and mixtures of probes could be used to identify presence of variants of the gene even for heterozygous types.

Station: Iowa (Iowa State University)

PI: Chenxu Yu,

Investigators/Participants: Chenxu Yu, Jonathan Claussen

Accomplishments:

Objective 1. Develop new technologies for characterizing fundamental nanoscale processes.

In this period, we continued evaluating production and presence of carbon nanoparticles in foods, and their fluorescence and bioluminescence properties. It furthered our understanding of naturally occurring nanoscale processes in food matrix which may lead to better utilization of these nano-phenomena.

Objective 2. Construct and characterize self-assembled nanostructures.

We continued working on development of nano-vaccines using self-assembled nanostructures as carriers and cloned viral protein sigma 1 as a target recognition mechanism for improved delivery.

Objective 3. Develop devices and systems incorporating microfabrication and nanotechnology.

We continued our work on SERS imaging to achieve rapid detection of pathogens in low moisture foods. We also continued investigating the potential of using portable Raman imaging to diagnose Chronic Downing disease in deer.

Station: Kentucky (University of Kentucky)

PI: Olga Tsyusko (University of Kentucky, College of Agriculture Food and Environment, Department of Plant and Soil Sciences)

Investigators/Participants: Olga Tsyusko: Ph.D. Students: Anye Wamucho, Stuart Lichtenberg, and Jarad Cochran; Collaborators: Evangelyn Alocilia (NC 1194 member), Jason Unrine, Isabel Escobar, Andrew Morris, Claus Svendsen, Carolin Shultz and David Spurgeon

Accomplishments:

The ongoing projects in my laboratory can be divided into four categories:

Toxicity and mechanisms from exposure to pristine and environmentally transformed metal and metal oxide nanomaterial. Together with my Ph.D. students, Daniel Starnes and Anye Wamucho, we examined bioavailability, toxicity and its underlying mechanisms from exposure of a model organism soil nematode Caenorhabditis elegans, to silver and zinc oxide nanomaterials in their pristine and environmentally modified (aged) forms. Among transformed nanomaterials were silver sulfide, zinc phosphate and zinc sulfide. We were among the first to examine multigenerational effects from the exposure to silver nanomaterials. Our previous studies demonstrated that exposure to pristine silver nanomaterials induced reproductive sensitivity in as early as second generation, which persisted for nine generations without recovery, even when exposure has been stopped. We investigated whether genomic mutations or/and epigenetic modifications can explain such heightened sensitivity. While increase in germline mutations were observed after exposures to all silver treatments, changes in histone methylation (at H3K4me2 and H3K9me3) demonstrate stronger correlation with the reproductive toxicity.

Efficiency and non-target effect of nanocomposites. In this research we examine efficiency and non-targeted toxicity of chitosan/dsRNA polyplex nanoparticles using C. elegans. We demonstrate that chitosan/dsRNA polyplex nanoparticles can knockdown targeted gene effectively than naked dsRNA. In addition,  we show that chitosan/dsRNA polyplex nanoparticles introduce dsRNA into cells through clathrin-mediated endocytosis, which is a different mechanism from canonical pathway via sid-1 and sid-2. Finally, our results suggest that  chitosan, as either polyplex nanoparticles or alone, downregulates the expression of myosin.

Examining toxicity of phosphorene and its potential for degradation/removal of PFAS from drinking water.  The third project focuses on examining toxicity of 2D nanomaterial phosphorene on C. elegans with the purpose of developing “safe” phosphorene membranes. The overarching goal of this project is to develop novel nanocomposite membranes with minimal toxicity and to test their potential to remove per- and polyfluoroalkyl substances (PFAS). The specific aims are 1) to test in vivo toxicity of phosphorene nanomaterials in a free form and in filtrate after nanoparticles are embedded into the membrane on C. elegans and 2) relying on phosphorene’s photocatalytic properties, to degrade PFAS, that accumulate on the membrane surface, and examine toxicity of PFAS and their breakdown products to C. elegans.

Testing efficiency of iron oxide nanomaterials against antimicrobial resistant (AMR) bacteria in C. elegans. This is in collaboration with NC 1194 member Dr. Evangelyn Alocilja.  My new PhD student, Jarad Cochran, focuses on testing toxicity of antibiotic resistant and non-resistant strain Klebsiella pneumoniae before and after treatment with iron oxide nanomaterials to C. elegans. Currently we are examining toxicity (mortality, reproduction and internal ROS production) of iron oxide nanomaterials to C. elegans. The objective is to identify concentration which will not induce toxicity responses at the examined endpoints in C. elegans. After that both strains of K. pneumoniae will be treated to the “safe” for C. elegans concentration of iron oxide nanoparticles and will be fed to C. elegans to examine efficiency of the iron oxide nanomaterials against the AMR K. pneumoniae.

Station: Michigan (Michigan State University)

PI: Dr. Evangelyn Alocilja, Professor, Biosystems and Agricultural Engineering

Accomplishments:

We develop magnetic and gold nanoparticles for bacterial extraction and detection in complex matrices for health, food safety, and water quality. We have optimized our newly synthesized magnetic nanoparticles that can be used to isolate and concentrate pathogenic bacteria from food, water, and clinical samples. We have optimized our dextrin-capped gold nanoparticles for colorimetric signal generation in genomic DNA detection. We are conducting studies to determine the concentration effect of our magnetic nanoparticles in human, foodborne, and waterborne pathogens. We are developing new antimicrobial nanoparticles to help reduce foodborne illness.

I received a Balik Scientist Award from the Philippine government in 2018 which funded my travel to present my research as well as allow me to establish and strengthen new research collaborations. I received a travel fellowship from Ehime University which allowed me to travel to Japan and present my research as well as establish new research collaboration with faculty from Ehime University. We published 9 peer-reviewed journal papers and made 26 conference/meeting presentations.

Station: Missouri (University of Missouri)

PI: Mengshi Lin, Professor, Food Science Program, University of Missouri

Investigators/Participants: Fouad Alsammarraie (PhD student), Lin Sun (PhD student)

Accomplishments:

Our objectives are to develop new technologies for characterizing fundamental nanoscale processes; construct and characterize self-assembled nanostructures; and develop devices and systems using nanotechnology.

In this reporting period, we developed a nanocomposite based on nanofibrillar cellulose (NFC) integrated with gold-silver (core-shell) nanoparticles (Au@Ag NPs) as a novel surface-enhanced Raman spectroscopy (SERS) substrate. SERS performance of NFC/Au@Ag NPs nanocomposite was tested by 4-mercaptobenzoic acid (4-MBA). Cellulose nanofibril network was a suitable platform that allowed Au@Ag NPs to be evenly distributed and stabilized over the substrate, providing more SERS hotspots for the measurement. Two pesticides, thiram and paraquat, were successfully detected either individually or as a mixture in lettuce by the nanocomposite. Strong Raman scattering signals for both thiram and paraquat were obtained with the Raman intensities ~8 times higher than the ones that were acquired by NFC/Au NPs nanocomposite. Characteristic peaks were clearly observable in all SERS spectra even at a low concentration of 10 µg/L of pesticides. Limit of detection values of about 71 µg/L and 46 µg/L were obtained for thiram and paraquat, respectively. Satisfactory SERS performance, reproducibility and sensitivity of NFC/Au@Ag NPs nanocomposite validate its applicability for real-world analysis to monitor pesticides and other contaminants in complex food matrices within a short acquisition time.

Station: New York (Cornell University)

PI:  Margaret Frey

Investigators/Participants: Soshana Smith, Katarina Goodge, Michael Delaney

Accomplishments:

We continue to work on producing submicron fibers with surface properties suitable to capture and concentrate target chemicals, pathogens or biological molecules from liquids.  Our latest efforts focus on attaching specific anti-bodies to nanofiber surfaces and measuring capture capabilities from fluids and in microfluidic channels. Two types of fibers are described below.

Biotin Functionalized Silver-Doped Carbon Nanofibers for Selective Capture of E.coli in Microfluidic Systems: Silver-doped carbon nanofibers are used as the base of material for the selective capture of E.coli in microfluidic systems. As-spun PAN fiber, PAN based carbon nanofibers, as-spun PAN/AgNO3, Ag doped nanofibers,and Ag doped nanofibers coated with poly(3,4-ethylenedioxythiophene): polystyrenesulfonate  (PEDOT:PSS) were studied using various characterization techniques. Energy-dispersive X-ray spectroscopy was used to confirm the confirm the presence of certain elements in the fibers such as the sulfur in PEDOT:PSS. While 4 probe testing was used to study the conductivity of the various fibers. After various tests, it was determined that the PEDOT: PSS coating was not needed to ensure the success of the future steps. Therefore silver-doped carbon nanofibers were chosen as the desired substrate. Antibodies were immobilized on the surface of the silver-doped nanofibers via a 3-stepprocess. The negatively charged silver particles present on the surface of the nanofibers provide a suitable substrate for positively charged biotinylated poly(l-lysine)-g-poly(ethylene glycol) (PLL-g-PEG)FITC to attach. PLL-g-PEG FITC was initially used to get a visual conformation that the conjugate attachment worked using confocal microscopy. A similar conjugate, PLL-g-PEG biotin, was used for future experiments to take advantage of the strong affinity of biotin for streptavidin. After the addition of the conjugate, streptavidin and a biotinylated anti E coli antibody are then added system to selectively capture E. coli cells with high efficiency. We will demonstrate the fibers ability for isolation, detection, sequential collection of E coli.

Functionalizing Cellulose-based Nanofibers Using “Click” Chemistry: This project aims to address the ongoing challenges of disease diagnostic technology by leveraging the high specific surface area and functionality of nanofibers to design highly sensitive and accurate bioassays. Specifically, the surface chemical modification of cellulose acetate nanofibers was studied to determine the most efficient and effective method to use as the intermediate step in attaching functional molecules via click chemistry. Cellulose acetate was electrospun into nanofibers that were chemically modified either with or without a deacetylation step. The as-spun and regenerated fibrous mats have alkyne moieties covalently attached to the nanofibers that selectively “click” with corresponding azide biotin conjugates. Two methods of alkyne-to-fiber attachment were tested and optimized to achieve practically useful degrees of substitution of the respective moieties on the repeat units. The Huisgen [3+2] copper-catalyzed azide-alkyne cycloaddition (CuAAC) “click” reaction was used to model the potential of click degree of substitution by using biotin as a model biomolecule and streptavidin-FITC as a fluorescence marker. FTIR and Raman were used as initial characterization techniques to confirm successful reaction products. EDX was used to map the biotin clicked onto the mats as well as confocal microscopy to confirm the distribution and degree of substitution of the biotin conjugate.

Station: South Carolina (Clemson University)

PI: Dr. Tzuen-Rong Jeremy Tzeng

Accomplishments:

Pathogen attachment is a complex phenomenon and vital process for successful initiation of infection in the host. Bacterial pathogens utilize two primary mechanisms to adhere onto host cells, namely carbohydrate-protein recognition and protein-protein interaction. The adhesion structures have a high degree of preference for a particular host-cell receptor. We have developed nanoparticles functionalized with specific receptors and evaluated their ability for selective binding and killing of pathogens. During this report period, we have developed iron-oxide nanoparticles functionalized with carbohydrates specific for Neisseria gonorrhoeae and conducted both in vitro and in vivo mouse models. In addition, we have developed and evaluated X-ray excited luminescent chemical imaging (XELCI) for non-invasive imaging of implant related infections.

Station: South Dakota (South Dakota State University)

PI: Zhengrong Gu

Investigators/Participants:

Undergraduates: Nelson, Zebadiah Peter; Emily Leupp; VanWell, Elliot; Held, Logan;

PhD students: Hummel, Matthew, Shun Lu.

Accomplishments:

We developed new technologies for characterizing fundamental nanoscale processes. We developed electrochemical analysis, including impedance, differential pulse voltammetry of biomolecule interactions such as DNA chains hybridization, antibody-antigens interaction in nano-scale.

We constructed and characterized self-assembled nanostructures. Nickel oxide nanoparticles anchored on three-dimensional (3D) carbonized eggshell membrane (NiO/C) are synthesized by a green hydrothermal approach followed by a pyrolysis in the nitrogen atmosphere at 500 ℃. The introduction of eggshell membrane (ESM) provides a microreactor for the formation of Ni(OH)2/ESM in which Ni(OH)2 is formed by adsorption of ESM during hydrothermal process, which not only exposes more catalytic activity edge but also transfers biowaste into useful nanomaterials. The carbonized ESM exhibited its robust and porous structure after heat treatment, which provided a stable support and electronic transmission channels that endowing the Ni2+ to Ni3+ pre-oxidation process to boost urea electrooxidation and detection. Various physical characterization methods were applied to confirm the structure, consistence and properties of the as-synthesized sample.We also prepared kappa-carrageenan (KC) and hierarchical porous activated carbon (HPAC) auto-assembled nano-composite. The alkali lignin derivate HPAC was synthesized with a confidential atmospheric plasma processes. Due to KC’s unique interlocking helix-structure, the KC-HPAC structured composite demonstrated high surface area and highly porous structure, in which the KC helical strands extend from the surface of HPAC. TEM images also demonstrated both large, irregular dispersed pores of >250nm and nano-sized, regularly interspersed pores in the prepared HPAC and KC-HPAC composite. The hierarchical pore structure of HPAC is attributed to the rapid conversion in atmospheric plasma process. KC’s pores are a result of the spaces left between the interlocking helices of the linear disaccharide, which auto-assembles to this conformation after dissolving in water and subsequently constituting as a gel.

We developed devices and systems incorporating microfabrication and nanotechnology. The electrocatalytic performance of NiO/C electrode toward urea oxidation and urea detection in alkaline solution was evaluated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), linear sweep voltammetry (LSV), square wave voltammetry (SWV) and chronoamperometry (CA). Results show that the as-prepared electrode possesses an onset oxidation potential of 1.47 V (vs. RHE) and a peak current density of 1.86 mA/cm² in 1.0 M KOH and 0.33 M urea solutions. Moreover, NiO/C modified electrode exhibits an outstanding performance for urea determination with two linear ranges of 0.05-2.5 mM, a limit of detection (LOD) of ∼20 μM, and fast response time (within 7 s). Furthermore, the stability and anti-interference performance of the developed electrochemical sensor was also measured. The good selectivity of the electrochemical urea sensor in a diluted urea sample containing different interfering species, such as glucose (Glu), Na+ ions, K+ ions, and uric acid (UA) were verified with stairs response towards urea of the current electrode when successive added urea, while insignificant responses to the other interfering species. Significantly, this work offered a green method for fabricating 3D nanostructure by using a biowaste ESM as template, setting up a typical example for producing new value-added nanomaterials with urea electrooxidation. Multiple HPAC-KC composite were tested as electrode for detecting dopamine in solution. It was hypothesized that due to HPAC’s higher conductivity than KC, the limit of detection would be higher with an increasing proportion of HPAC. This hypothesis was confirmed. As predicted, having a higher proportion of conductive HPAC would both provide a higher amplitude of peak current and more sites for KC helices to adsorb physically. As a result, the limit of detection with a S/N ratio of 3 was highest for 2500:1 HPAC: KC at 0.4 umol/L and a linear range of 1 umol /L– 600 umol/L. Interestingly, the limit of detection did not scale uniformly with the increasing concentration of HPAC. Instead, the second highest limit of detection was determined to be 100:1 HPAC: KC. The lowest limit of detection was encountered with the 500:1 HPAC: KC film. One potential explanation for this phenomenon could lie in the orientation of the KC helices on the HPAC’s surface. KC’s helices best film forming occurs in 1.0-2.5% solutions by mass in 1% acetic acid. In the 200:1-1250:1 HPAC: KC range, this interaction between acetic acid and KC is disrupted due to the HPAC’s own interactions with KC and acetic acid. By increasing the ratio, HPAC’s interactions with KC dominate, while lower ratios allow KC to interact with acetic acid and form more stable, protonated gels, which also benefit detection of dopamine.

Furthermore, the innovative HPAC-KC platform is competitive with other reported biosensors of dopamine in terms of linear range and limit of detection. A major advantage the HPAC: KC film has over the majority of dopamine sensors is its simple method of preparation and low-cost materials. Notably, no adhesive polymer such as PTFE, Nafion, or PVDF was necessary in adhering the film to the surface of the glass carbon electrode. Instead, the adhesive properties of the composite itself were relied upon with a high degree of success.

Station: Utah (Utah State University)

PI: Anhong Zhou

Investigators/Participants: Graduate students: Han Zhang, Wei Zhang; Undergraduates: Reem Ghabayen, Kamila Khamidova

Accomplishments:

Understanding of the DNA-mediated electron transfer characteristics on sensor surface plays essential roles in design of highly sensitive DNA biosensors. We have applied a variety of electrochemical techniques, including cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy to quantitatively assess the electron transfer behaviors of each step of construction of DNA biosensors, such as probe DNA immobilization, probe-target hybridized surface, capture probe DNA hybridization, and electroactive ALP-linked enzyme. We obtained the optimal sensor design based on these assessment results.

Starting with the first step of immobilizing probe DNA (thiolated oligonucleotides), the construction of DNA biosensor includes multiple self-assembled steps to finally form highly specific DNA structure on sensor surface for recognizing the target DNA sequence. The experimental conditions of each self-assembled step are optimized. The overall performance such as sensitivity, selectivity, detection limit, and linear range have been evaluated for hybridization with complementary, single-base, three-base, and five-base mismatched target sequences.

A prototype microfluidic device was fabricated using photolithograph techniques, and tested in detection of various target sequences (with different base-mismatch) to achieve satisfactory results. Our DNA biosensor for the first time realized the separation of the probe/target DNA immobilized sensor part (disposable) from the detection of electron-transfer sensor part (reusable), making it well suitable for multiplex detection of other species-specific target DNA with similar sequences (to test specificity).

In addition, to address the Objective#3, we initiated a collaboration with Prof. Heloisa Rutigliano’s group from USU Animal Science Department in using non-invasive Raman spectroscopy technique to characterize the nanoscale extracellular vesicles (EVs) isolated from different animal cell samples. Further, we also designed and fabricated a prototype of smartphone based colorimetric biosensor for the detection of blood glucose. We have tried it successfully with artificial urine samples, and we are testing this biosensor for detecting blood glucose in cows at different pregnancy stages.

In this past year, we have been successfully testing our DNA sensor with the target sequence specific to human genotype of Cryptosporidium. Our results indicate a detection of limit of target sequence with six times lower sensitivity, compared to the literature reported method.

Station: Virginia (Virginia Polytechnic Institute and State University)

PI: Chenming (Mike) Zhang

Investigators/Participants: Yi Lu (graduate student)

Accomplishments: We have constructed a chimeric protein based on Hepatitis B core antigen.  The chimeric protein is expressed in E. coli and purified by various chromatographic techniques.  The purified protein could self-assembled into virus like particles, which was characterized by scanning electron microscopy.  The virus-like particle is studied as a potential vaccine against a swine disease.

Planned Activities:

The NC-1194 members are working on a review article about antimicrobial resistance (AMR) that is a global public health challenge requiring a global multi-disciplinary approach.  Due to globalization, AMR anywhere is a threat to health everywhere. New antibiotic resistance mechanisms are spreading globally, threatening our ability to treat common infectious diseases, resulting in prolonged illness, disability, and death. In addition to the toll on human life, antibiotic-resistant infections add considerable avoidable costs to the already overburdened US health care system. Studies have estimated that, in the US, antibiotic resistance adds $20 billion in excess direct health care costs, with additional costs to society for lost productivity as high as $35 billion a year. Globally, failure to act on drug-resistant infections will lead to 10 million extra deaths a year and cost the global economy $100 trillion by 2050. The loss of effective antibiotic treatments will also undermine treatment of infectious complications in patients with other diseases. Many medical advances, such as joint replacements, organ transplants, cancer therapy, and others, are dependent on the ability to fight infections with antibiotics. If the ability to effectively treat those infections is lost, the ability to safely offer people the life-saving and life-improving modern medical advances will be lost with it. Thus, this paper will present scientific and engineering challenges of AMR in the human population, food and animal production, and the environment. The convergent multidisciplinary knowledge generated will be designed to stimulate new inquiries and challenge new discoveries toward AMR reduction.

The members who have expressed interest in this review article include: Evangelyn Alocilja, Olga Tsyusko, Jeremy Tzeng,  Paul Takhistov,  Chenxu Yu, Zhengrong Gu, Mengshi Lin, Carmen Gomes, Jonathan Claussen, Jeong-Yeol Yoon, Anhong Zhou, Yi-Cheng Wang, Eric McLamore, Yanbin Li,  Elizabeth Carraay.

Publications

Chung, S., Breshears, L.E., Yoon, J.-Y. *, Smartphone Near Infrared Monitoring of Plant Stress, Computers and Electronics in Agriculture, 2018, 154: 93-98.

Alouidor, B., Sweeney, R.E., Tat, T., Wong, R.K.* and Yoon, J.-Y. *, Microfluidic Point-of-care Ecarin Based Clotting and Chromogenic Assays for Monitoring Direct Thrombin Inhibitors, Journal of ExtraCorporeal Technology, 2019, 51: 29-37.

Klug, K.E., Jennings, C.M., Lytal, N., An, L., and Yoon, J.-Y. *, Mie Scattering and Microparticle Based Characterization of Heavy Metal Ions and Classification by Statistical Inference Methods, Royal Society Open Science, 2019, 6: 190001.

Sweeney, R.E., Nguyen, V., Alouidor, B., Budiman, E., Wong, R.K., and Yoon, J.-Y. *, Flow Rate and Raspberry Pi-based Paper Microfluidic Blood Coagulation Assay Device, IEEE Sensors Journal, 2019, 19(13): 4743-4751. Top 25 Most Downloaded IEEE Sensors Journal Papers in June 2019.

Chung, S., Breshears, L.E., Perea, S., Morrison, C.M., Betancourt, W.Q., Reynolds, K.A., and Yoon, J.-Y. *, Smartphone-based Paper Microfluidic Particulometry of Norovirus from Environmental Water Samples at Single Copy Level, ACS Omega, 2019, 4(6): 11180-11188. Highlighted in ACS News Release and more.

Tiffany-Heather Ulep, Alexander S. Day, Katelyn Sosnowski, Alexa Shumaker and Jeong-Yeol Yoon*, Interfacial Effect-based Quantification of Droplet Isothermal Nucleic Acid Amplification for Bacterial Infection, Scientific Reports, 2019, 9: 9629.

Matthew V. Bills, Brandon T. Nguyen and Jeong-Yeol Yoon*, Simplified White Blood Cell Differential: An Inexpensive, Smartphone- and Paper-Based Blood Cell Count, IEEE Sensors Journal, 2019, 19(18): 7822-7828.

M. Jenkins, B. E. Lee, S. Jun, J. Reyes-De-Corcuera, and E. S. McLamore, J. Electrochem. Soc., 166, B3056–B3065 (2019).

Lv J, Yang Z, Xu W, Li S, Liang H, Ji C, Yu C, Zhu B, Lin X, Relationships between bacterial community and metabolites of sour meat at different temperature during the fermentation, Int J Food Microbiol. DOI: 10.1016/j.ijfoodmicro.2019.108286, 2019.

Xiong X, He BY, Jiang D, Dong XF, Yu C and Qi H, Postmortem biochemical and textural changes in the sea cucumber Stichopus japonicus body wall (SJBW) during iced storage, LWT-Food Sci. Technol. DOI: 10.1016/j.lwt.2019.108705, 2019.

Jiang D, Bai Y, He BY, Sui Y, Dong XF, Yu C and Qi H, Improvement of gel properties of mackerel mince by phlorotannin extracts from sporophyll of Undaria pinnatifidai and UVA induced cross-linking, J. Textural Studies, DOI:10.1111/jtxs.12480, 2019.

Liu XY, Wang ZX, Zhang J, Song L, Li DY, Wu ZX, Zhu BW, Nakamura Y, Shahidi F, Yu C, and Zhou DY, Isolation and identification of zinc-chelating peptides from sea cucumber (Stichopus japonicus) protein hydrolysate, J. the Science of Food and Agriculture, DOI:10.1002/jsfa.9919, 2019.

Liu XY, Wang ZX, Yin FW, Liu YX, Qin NB, Nakamura Y, Shahidi F, Yu C, Zhou DY, Zhu BW, Zinc-Chelating Mechanism of Sea Cucumber (Stichopus japonicus)-Derived Synthetic Peptides, Marine Drugs, 17, 438, DOI:10.3390/md17080438, 2019.

Dong XF, Bai Y, Xu Z, Shi YX, Sun YH, Janaswamy S, Yu C, Qi H, Phlorotannins from Undaria pinnatifida Sporophyll: Extraction, Antioxidant, and Anti-Inflammatory Activities, Marine Drugs, 17, 434, DOI:10.3390/md17080434, 2019.

Xiong X, He BY, Jiang D, Koosis A, Yu C, Qi H, Postmortem biochemical and textural changes in the Patinopecten yessoensis adductor muscle (PYAM) during iced storage, Inter. J. Food Properties, 22, 1024-1034, 2019.

Sun H, Li DM, Jiang D, Dong XF, Yu C, Qi H, Protective polysaccharide extracts from sporophyll of Undaria pinnatifida to improve cookie quality, Food Measurement and Characterization, 13(1), 764-774, 2019.

Zhang XY, Jiang D, Li DM, Yu C, Dong XF, Qi H, Characterization of a seafood-flavoring enzymatic hydrolysate from brown alga Laminaria japonica, Journal of Food Measurement and Characterization, 13(2), 1185-1194, 2019.

Li JQ, Cao L, Li DM, Yu C, Tan MQ, Carbon dots from roasted mackerel (scomberomorus niphonius) for free radical scavenging, LWT-Food Sci. Technol., 111, 588-593, 2019.

Bao R, Gao N, Lv J, Ji CF, Liang HP, Li SJ, Yu C, Wang ZY, Lin XP, Enhancement of Torularhodin Production in Rhodosporidium toruloides by Agrobacterium tumefaciens-Mediated Transformation and Culture Condition Optimization, Journal of Agricultural and Food Chemistry 67 (4), 1156-1164, 2019.

Zhang, J, Hu J, Wang S, Lin X, Liang H, Li S, Yu C, Dong X, Ji C, Developing and Validating a UPLC-MS Method with a StageTip-Based Extraction for the Biogenic Amines Analysis in Fish, J Food Sci. 84(5),,1138-1144. doi: 10.1111/1750-3841, 2019.

Li, SJ, Yu C, Pan JF, Ma RC, Lin XP and Dong XP, Combined effects of aging and low temperature, long time heating on pork toughness, Meat Science, 150, 33-39. 2019.

Dong XP, Liu WT, Song X, Lin XY, Yu D, Yu C and Zhu BW, Characterization of Heat-Induced Water Adsorption of Sea Cucumber Body Wall, J. Food Science, 84(1), 92-100, 2019.

Hu J, Zhao TF, Li SJ, Wang ZY, Wen CR, Wang HT, Yu C, Ji CF, Stability, microstructure, and digestibility of whey protein isolate – Tremella fuciformis polysaccharide complexes, Food Hydrocolloids, 89, 379-385, 2019.

Mammadova N, Kokemuller R, Summers C, He Q, Ding S, Baron T, Yu C, Valentine R, Sakaguchi D, Kanthasamy A, Greenlee J and Greenlee MHW, Accelerated accumulation of retinal α-synuclein (pSer129) and tau, neuroinflammation and autophagic dysregulation in a seeded mouse model of Parkinson’s disease, Neurobiology of Disease, 121, 1-16, 2019.

Wamucho, A., Unrine, J. M., Kieran, T. J., Glenn, T. C., Schultz, C. L., Farman, M. L., Svendsen, C., Spurgeon, D. J.,  Tsyusko, O. V. (2019). Genomic mutations after multigenerational exposure of Caenorhabditis elegans to pristine and sulfidized silver nanoparticles. Environmental Pollution vol. 254, pp. 113078

Li, J., Rodrigues, S., Tsyusko, O. V., Unrine, J. M. (2019). Comparing plant-insect trophic transfer of Cu from lab-synthesized nano-Cu(OH)(2) with a commercial nano-Cu(OH)(2) fungicide formulation. Environmental Chemistry vol. 16, pp. 411-418.

Lichtenberg, S. S., Tsyusko, O. V., Palli, S. R., Unrine, J. M. (2019). Uptake and Bioactivity of Chitosan/Double-Stranded RNA Polyplex Nanoparticles in Caenorhabditis elegans. Environmental science & Technology  vol. 53, pp. 3832-3840.

Starnes, D., Unrine, J. M., Chen, C., + Lichtenberg, S., Starnes, C., Svendsen, C., Kille, P., Morgan, J. S., # Baddar, Z. E., Spear, A., Bertsch, P. M., Chen, K. C., * Tsyusko, O. V. (2019). Toxicogenomic responses of Caenorhabditis elegans to pristine and transformed zinc oxide nanoparticles. Environmental Pollution vol. 247, pp. 917-926.

Wamucho A., Heffley A.,  and Tsyusko* O.V. In Review. Multigenerational exposure of Caenorhabditis elegans to silver nanoparticles induces histone methylation changes. Submitted to Environmental Toxicology & Chemistry.

Gordillo CM, Gomez AV, Sanchez HP, Pryg K, Shinners J, Murray N, Munoz-SG, Bencomo AA, Gomez, AB, Janapa LG, Enriquez NR, Martin M, Romero NS, and Alocilja EC. 2018. Magnetic Nanoparticle-based Biosensing Asasy Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis. Biosensors, 8(4):128-141.

Alocilja EC, Sharief SA, Kriti N, and Chahal P. 2018. Combining Blockchain, DNA, and Nanotechnology for Product Authentication and Anti-Counterfeiting. Brand Protection Professional, (Dec. 20, 2018).

Matta LL and Alocilja EC. 2018. Carbohydrate Ligands on Magnetic Nanoparticles for Centrifuge-free Extraction ofPathogenic Contaminants in Pasteurized Milk. J Food Protection, 81(12):1941-1949. (Dec. 2018)

Zeeshan N, Daya KS, Tirumalai PS, and Alocilja E. 2018. Impedance and Magnetohydrodynamic Measurements for Label Free Detection and Differentiation of E. coli and S. aureus using Magnetic Nanoparticles. IEEE Transactions on NanoBioscience, 17(4):443-448. (Oct. 2018).

Matta LL, Karuppuswami S, Chahal P, and Alocilja EC. 2018. AuNP-RF Sensor: An innovative application of RF technology for sensing pathogens electrically in liquids (SPEL) within the food supply chain. Biosensors and Bioelectronics, 111:152-158.

Matta LL, Harrison J, Deol G, and Alocilja EC, 2018. Carbohydrate-functionalized Nano-Biosensor for Rapid Extraction of Pathogenic Bacteria Directly from Complex Liquids with Quick Detection Using Cyclic Voltammetry. IEEE Transactions on Nanotechnology, 17(5):1006-1013.

Matta LL and Alocilja EC. 2018. Emerging nano-biosensing with suspended MNP microbial extraction and EANP labeling. Biosensors and Bioelectronics, 117:781-793

Sun, L., Yu, Z., Lin, M.* 2019. Synthesis of polyhedral gold nanostars as surface-enhanced Raman spectroscopy substrates for measurement of thiram in peach juice. Analyst. 144, 4820-4825.

Yu, Z., Dhital, R., Wang, W., Sun, L., Zeng, W., Mustapha, A.*, Lin, M.* 2019. Development of multifunctional nanocomposites containing cellulose nanofibrils and soy proteins as food packaging material. Food Packaging and Shelf Life. 21, 100366.

Asgari, S., Saberi, A.H., McClements, D.J., Lin, M.* 2019. Microemulsions as nanoreactors for synthesis of biopolymer nanoparticles. Trends Food Sci. Technol. 86, 118-130.

Yu, Z., Wang, W., Dhital, R., Kong, F., Mustapha, M., Lin, M.* 2019. Antimicrobial effect and toxicity of cellulose nanofibril/silver nanoparticle nanocomposite prepared by an ultraviolet irradiation method. Colloids Surf. B. 180, 212-220.

Yu, Z., Wang, W., Kong, F., Lin, M.*, Mustapha, A.* 2019. Cellulose nanofibril/silver nanoparticle composite as an active food packaging system and its toxicity to human colon cells. Int. J. Biol. Macromol. 129, 887-894.

An oral presentation and a poster presentation were made at the International Fiber Society meeting in Austin, TX, October 27-30, 2019.  Manuscripts are being prepared on both projects and will be submitted for publication early in 2020.

An implanted pH sensor read using radiography. Md. Arifuzzaman,  Paul W. Millhouse,  Yash Raval*,  Thomas B. Pace,  Caleb J. Behrend,  Shayesteh Beladi Behbahani*,  John D. DesJardins,  Tzuen-Rong J. Tzeng  and  Jeffrey N. Anker. Analyst, 2019, April 23; 144 (9): 2984-2993

Lu S., Hummel M., Gu Y., Gu, Z*, Trash to treasure: A novel chemical route to synthesis of NiO/C for hydrogen production. 2019. Intern. J. Hydrogen Energy 44 (31), 16144-16153.

Yan Gu, Matthew Hummel, Zhengrong Gu*, Kasiviswanathan Muthukumarappan, Zhendong Zhao, 2019, Synthesis and Characterization of Allyl Terpene Maleate Monomers, submitted to Scientific Reports in 2019 July, under reviewing after revision in 2019 Oct.

Shun Lu; Zhengrong Gu*; Xiaoteng Liu; Matthew Hummel; Yue Zhou; Keliang Wang; Yucheng Wang, 2019, A high-performance nickel oxide on carbonized eggshell membrane catalyst for electrocatalytic urea oxidation and detection, submitted to Applied Catalysis B:  Environmental 2019 August, under reviewing.

Shun Lu; Zhengrong Gu*; Xiaoteng Liu; Matthew Hummel; 2019, Synthesis of Au@ZIF-8 nanoparticles for enhanced electrochemical detection of dopamine, submitted to the Journal of The Electrochemical Society 2019 Oct., under reviewing.

Matthew Hummel, Shun Lu, Nelson, Zebadiah Peter, Zhengrong Gu*, 2019, Graphene-biopolymer composite electrode for detecting dopamine, submitted to Biosensors and Bioelectronics in 2019 August, under reviewing.

Matthew Hummel, Shun Lu, Zhengrong Gu*, Erin Lee, Emily Leupp, 2019, High energy room temperature synthesis graphene from lignin – application in supercapacitor, submitted to Journal of Power Sources in 2019 August, under reviewing.

Hoda ilkhani, Han Zhang, Anhong Zhou, “A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptosporidium DNA biosensor”, Sensors & Actuators: B. Chemical, 2019, 282: 675–683.

Han Zhang, Ethan Smith, Wei Zhang, Anhong Zhou, “Inkjet printed microfluidic paper-based analytical device (uPAD) for glucose colorimetric detection in artificial urine”, Biomedical Microdevices, 2019, 21:48.

Han Zhang, Ana Caroline Silva, Wei Zhang, Heloisa Rutigliano, Anhong Zhou, “Raman spectroscopy characterization of extracellular vesicles derived from bovine placenta and peripheral blood mononuclear cells”, Analyst, 2019 (revision submitted).