Brief Summary of Minutes of Annual Meeting

This is a report of accomplishment of NC1194 for the period October 1, 2017 through September 30, 2018 from participating institutions from different states. The findings have been disseminated to the scientific community via seminars, national/international conferences, manuscripts, and web sites. The objectives of this project are:

1.     Develop new technologies for characterizing fundamental nanoscale processes

2.     Construct and characterize self-assembled nanostructures

3.     Develop devices and systems incorporating microfabrication and nanotechnology

4.     Develop a framework for economic, environmental and health risk assessment for nanotechnologies applied to food, agriculture and biological systems

5.     Develop/improve education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment

6.     Improve academic-industry partnership to help move the developed technologies to commercialization phase

Station: University of Arkansas

PI: Yanbin Li

Investigators/Participants: Ronghui Wang (Research Scientist), Zach Callaway (PhD student), Wenqian Wang (PhD student), Xinge Xi (PhD student), Xiaofan Yu (PhD student), America Sotero (MS student)

Objective(s) Addressed: #2 & 3

Summary of Work:

Various nanoscale materials, including magnetic nanoparticles, silk nanofibers, gold nanorods, nanochannels, were synthesized or fabricated, characterized, and used in development of new biosensors for the rapid detection of pathogenic bacteria, viruses, toxic agents, and antibiotics residues in agricultural and food products. Enzymes, antibodies, aptamers and DNA probes were selected as biosensing materials and electrochemical, optical, QCM transdusing methods were employed in the design of novel biosensors. The nanomaterials-based biosensors were also integrated with microfluidic chips-based sample treatment and measurement and cell phone-based data acquisition, processing, and transmission.

This project provided an opportunity for training four Ph.D. students and one M.S. student in the areas of biosensors and nanotechnology. The project also enhanced our international collaboration with Zhejiang University and China Agricultural University on a project funded by Walmart Foundation. We made 15 presentations to the industry and scientific communities at professional conferences such as ASABE, IAFP, and IBE annual meetings as well as Biosensors 2018. We also published 11 articles in different journals including Biosensors & Bioelectronics, Sensors & Actuators, Journal of Food Protection, Electroanalysis, Journal of Materials Chemistry, Journal of Electroanalytical Chemistry, Journal of Biotechnology, etc. 

Outputs:

1. Cao, L.L., Q. Zhang, H. Dai, Y.C. Fu, and Li. 2018. Separation/concentration-signal-amplification in-one method based on electrochemical conversion of magnetic nanoparticles for electrochemical biosensing. Electroanalysis 30(3):517-524. DOI: 10.1002/elan.201700653
2. Dai, H., Y.Q. Li, Y.C. Fu, and Li. 2018. Enzyme catalysis induced polymer growth in nanochannels: A new approach to regulate ion transport and to study enzyme kinetics in nanospace. Electroanalysis 30(2):328-335 (available online Dec. 18, 2017). DOI:10.1002/elan.201700703
3. Dai, H., Y.Q. Li, Q. Zhang, Y.C. Fu, and Li. 2018. A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine. RSC Advances 8:33960-33967. DOI: 10.1039/c8ra06347h
4. Hu, Q.Q., R.H. Wang, H. Wang, M.F. Slavik and Li. 2018. Selection of acrylamide-specific aptamers by a quartz crystal microbalance combined SELEX method and their application in rapid and specific detection of acrylamide. Sensors and Actuators: B: Chemical 273:220-227. doi.org/10.1016/j.snb.2018.06.033
5. Li, Z.S., G.S. Zhou, H. Dai, M.Y. Yang, Y.C. Fu, Y.B. Ying, and Li. 2018. Biomineralization-mimetic preparation of hybrid membranes with ultra-high load of pristine metal-organic frameworks grew on silk nanofibers for hazards collection in water. Journal of Materials Chemistry A 6(8):3402-3413 (published online on December 5, 2017). DOI:10.1039/C7TA06924C
6. Wang, H., L.J. Wang, Q.Q. Hu, R.H. Wang, Li and M. Kidd. 2018. Rapid and sensitive detection of Campylobacter jejuni in poultry products using a nanoparticles-based piezoelectric immunosensor integrated with magnetic immunoseparation. Journal of Food Protection 81(8):1321-1330. doi:10.4315/0362-028X.JFP-17-381
7. Wang, L.J., R.H. Wang, H. Wei, and Li. 2018. Selection of aptamers against pathogenic bacteria and their diagnostics application. World Journal of Microbiology and Biotechnology 34:149. doi.org/ 10.1007/s11274-018-2528-2
8. Xu, C.N., L.Y. Lan, Y. Yao, J.F. Ping, Li, and Y.B. Ying. 2017. An unmodified gold nanorods-based DNA colorimetric biosensor with enzyme-free hybridization chain reaction amplification. Sensors & Actuators: B. Chemical. 273:642-648. doi.org/:10.1016/j.snb.2018.06.035
9. Yu, X.F., F. Chen, R.H. Wang, and Li. 2018. Whole-bacterium SELEX of DNA aptamers for rapid detection of E. coli O157:H7 using a QCM sensor. Journal of Biotechnology 266:39-49. (available online, Dec. 22, 2017). doi.org/10.1016/j.jbiotec.2017.12.011
10. Zhang, Q., L. Zhang, H. Dai, Z.S. Li, Y.C. Fu, and Li. 2018. Biomineralization-mimetic preparation of robust metal-organic frameworks biocomposites film with high enzyme load for electrochemical biosensing. Journal of Electroanalytical Chemistry 823:40-46. doi.org/10.1016/j.jelechem.2018.04.015
11. Zheng, Y., G.Z. Cai, S.Y. Wang, M. Liao, Y. Li, and J.H. Lin. 2019. A microfluidic colorimetric biosensor for rapid detection of Escherichia coli O157:H7 using gold nanoparticle aggregation and smart phone imaging. Biosensors & Bioelectronics 124-125: 143-149. doi.org/10.1016/j.bios.2018.10.006

Impacts:

These nanomaterials-based biosensors have shown their potential to be applied to the in-field or online detection of biological and chemical agents in agricultural and food products for ensuring food quality and safety. The implementation of these biosensors may provide close to real-time data in biodetection for the development of smart agricultural and food systems based on big data, machine learning and artificial intelligence.

Station: University of Arizona

PI: Dr. Jeong-Yeol Yoon

Objective(s) Addressed: #3

Summary of Work:

There is a growing need to develop a handheld, smartphone-based biosensor that can detect the type and concentration of pathogens from myriads of food (fresh produce and meat) and water (waste and irrigation) samples, as well as urine, blood, and tissue samples from animal and human subjects. These biosensors must be designed and manufactured to be easy-to-use, all-in-one, and extremely sensitive (down to single cell level or picogram protein level).

Outputs:

1. Kattika Kaarj, Patarajarin Akarapipad and Jeong-Yeol Yoon, "Simpler, Faster, and Sensitive Zika Virus Assay Using Smartphone Detection of Loop-mediated Isothermal Amplification on Paper Microfluidic Chips," Scientific Reports, 2018, 8: 12438. [Aug. 20, 2018]
2. Tiffany-Heather Ulep and Jeong-Yeol Yoon, "Challenges in Paper-Based Fluorogenic Optical Sensing with Smartphones," Nano Convergence, 2018, 5: 14. [May 4, 2018]
3. Katherine E. Klug, Kelly A. Reynolds and Jeong-Yeol Yoon, "A Capillary Flow Dynamics-Based Sensing Modality for Direct Environmental Pathogen Monitoring," Chemistry - A European Journal, 2018, 24(23): 6025-6029. Hot Paper. Inside Cover. Highlighted in ChemistryViews Magazine. [Feb. 5, 2018]
4. Cayla Baynes and Jeong-Yeol Yoon, "µPAD Fluorescence Scattering Immunoagglutination Assay for Cancer Biomarkers from Blood and Serum," SLAS Technology (formerly JALA - Journal of Laboratory Automation), 2018, 23(1): 30-43. [Feb. 2018]
5. Soohee Cho, Tu San Park, Kelly A. Reynolds and Jeong-Yeol Yoon, "Multi-Normalization and Interpolation Protocol to Improve Norovirus Immunoagglutination Assay from Paper Microfluidics with Smartphone Detection," SLAS Technology (formerly JALA - Journal of Laboratory Automation), 2017, 22(6): 609-615. [Dec. 2017]
6. Robin E. Sweeney and Jeong-Yeol Yoon, "Angular Photodiode Array-Based Device to Detect Bacterial Pathogens in a Wound Model," IEEE Sensors Journal, 2017, 17(21): 6911-6917. [Nov. 1^st, 2017]

Station: Clemson University

PI: Dr. Tzuen-Rong Jeremy Tzeng

Objective(s) Addressed: #3

Summary of Work:

Pathogen attachment is a complex phenomenon and vital process for successful initiation of infection in the host. Bacterial pathogens utilize two primary mechanisms to adhere onto host cells, namely carbohydrate-protein recognition and protein-protein interaction. The adhesion structures have a high degree of preference for a particular host-cell receptor. We have developed nanoparticles functionalized with specific receptors and evaluated their ability for selective binding and killing of pathogens.

Outputs:

1. Revisit of wall-induced lateral migration in particle electrophoresis through a straight rectangular microchannel: Effects of particle zeta potential. Liu, Zhijian & Li, Di & Saffarian, Maryam & Tzeng, Tzuen‐Rong & Song, Yongxin & Pan, Xinxiang & Xuan, Xiangchun. Electrophoresis, (2018), 10.1002/elps.201800198.
2. Multianchored Glycoconjugate‐Functionalized Magnetic Nanoparticles: A Tool for Selective Killing of Targeted Bacteria via Alternating Magnetic Fields. Raval* YS, Fellows BD, Murbach J, Cordeau Y, Mefford OT, Tzeng TJ. Advanced Functional Materials, 2017, 27 (26): 1701473

Station: University of Florida

PI: Bin Gao

Objective(s) Addressed: #1 & 2

Summary of Work:

In addition to develop new technologies to characterize and understand fundamental nanoscale processes, we have also explored the environmental applications and implications of nanotechnology. The project has provided training and professional development opportunities to graduate and undergraduate students. The results have been published in several peer-reviewed journal articles and been presented in professional meetings and conferences. Furthermore, the findings from the research project was also integrated with education by training graduate and undergraduate students with a diverse array of backgrounds. 

Outputs:

1. Suthar, B. Gao. 2017. Use of nanotechnology against heavy metals present in water. In: A.M. Grumezescu, ed. Water Purification, 75-118.London, UK, Elsevier.
2. Wang, B. Gao, A.R. Zimmerman, X.Q. Lee. Impregnation of multiwall carbon nanotubes in alginate beads dramatically enhances their adsorptive ability to aqueous methylene blue. Chemical Engineering Research & Design, 2018. 133, 235-242.
3. Wang, B. Gao, Y. Wan. Comparative study of calcium alginate, ball-milled biochar, and their composites on methylene blue adsorption from aqueous solution. Environmental Science and Pollution Research, 2018. doi: 10.1007/s11356-018-1497-1.
4. X. Sun, S.N. Dong, Y.Y. Sun, B. Gao, W.C. Du, H.X. Xu, J.C. Wu. Graphene oxide-facilitated transport of levofloxacin and ciprofloxacin in saturated and unsaturated porous media. Journal of Hazardous Materials, 2018. 348, 92-99.
5. L. Gao, Y.M. Ma, Y.M. Zhou, H.H. Song, L. Li, S.H. Liu, X.Q. Liu, B. Gao, C.Z. Liu, K.P. Zhang. High photoluminescent nitrogen-doped carbon dots with unique double wavelength fluorescence emission for cell imaging. Materials Letters, 2018. 216, 84-87.
6. S. Wang, Y.X. Zhou, S.W. Han, N. Wang, W.Q. Yin, X.Q. Yin, B. Gao, X.Z. Wang, J. Wang. Carboxymethyl cellulose stabilized ZnO/biochar nanocomposites: Enhanced adsorption and inhibited photocatalytic degradation of methylene blue. Chemosphere, 2018. 197, 20-25.
7. Wang, B. Gao, D.S. Tang, C.R. Yu. Concurrent aggregation and transport of graphene oxide in saturated porous media: Roles of temperature, cation type, and electrolyte concentration. Environmental Pollution, 2018. 235, 350-357
8. Wang, B. Gao, D.S. Tang, H.M. Sun, X.Q. Yin, C.R. Yu. Effects of temperature on aggregation kinetics of graphene oxide in aqueous solutions. Colloids and Surfaces A-physicochemical and Engineering Aspects, 2018. 538, 63-72.

Station: University of Florida

PI: Eric S. McLamore

Investigators/Participants:

Eric S. McLamore, Associate Professor, Agricultural and Biological Engineering Department, Institute of Food and Agricultural Sciences

Bin Gao, Professor, Agricultural and Biological Engineering Department, Institute of Food and Agricultural Sciences

Bruce Welt, Professor, Agricultural and Biological Engineering Department, Institute of Food and Agricultural Sciences

John K. Schueller, Professor, Mechanical Engineering Department

Objective(s) Addressed: #3, 4, 5, & 6

Summary of Work:

Our research at UF in 2018 addressed objectives 3-6, as outlined below. Our station attended the 2018NC1194 meeting, as well as the Gordon Research Conference on Nanotechnology funded by NIFA. Our station also attended annual SPIE, IBE, ASABE, and IFT meetings. Research products were disseminated through training workshops in the US and abroad, conference papers/presentations and peer reviewed journal articles at all meetings.

Objective 3) Develop devices and systems incorporating microfabrication and nanotechnology. We published six articles in 2018 on the development and application of smart nano/micro sensor systems. These included nanomaterials applied to commercial screen printed electrodes, sensors developed on novel flexible carbon circuits, and post hoc analysis systems using a smart phone.

Objective 4) Develop a framework for economic, environmental and health risk assessment for nanotechnologies applied to food, agriculture and biological systems. Together with indigenous communities, we applied new nanosensors for studying mercury toxicity in rural Colombia and we also worked with plant physiology labs and food packaging labs to apply our sensors, establishing new stakeholders for the technologies developed by NC1194.

Objective 5) Develop/improve education and outreach materials on nanofabrication, sensing, systems integration and application risk assessment. We held workshops and disseminated training manuals with high school teachers in the USA (Florida) and also abroad (Colombia) for applying sensors to measure water quality and plant health.

Objective 6) Improve academic-industry partnership to help move the developed technologies to commercialization phase. Our group in Florida signed three CDA with major companies to develop and test sensor technologies as well as explore commercialization of our technologies.

The McLamore lab trained 14 undergraduate students, 3 PhD students, 3 high school student, and 2 high school teachers on nanotechnology and biosensors through grants provided by USDA/NSF.

Outputs:

1. Ding, S., C. Mosher, X.Y. Lee, S. Das, A. Cargill, X. Tang, B. Chen, E.S. McLamore, C. Gomes, J.M. Hostetter (2017). Rapid and Label-free Detection of Interferon Gamma via an Electrochemical Aptasensor Comprised of a Ternary Surface Monolayer on a Gold Interdigitated Electrode Array. ACS Sensors, 2(2): 210-217.
2. Garland, N.T., E.S. McLamore, N.D. Cavallaro, D. Mendivelso-Perez, E.A. Smith, D. Jing, J.C. Claussen (2018). Flexible Laser-Induced Graphene for Nitrogen Sensing in Soil. Advanced Functional Materials. 10 (45): 39124–39133. DOI: 10.1021/acsami.8b10991
3. Abdelbasir, S.M. S.M. El-Sheikh, V.L. Morgan, H. Schmidt, L.M. Casso-Hartmann, D.C. Vanegas, I. Velez-Torres, E.S. McLamore (2018). Graphene-anchored cuprous oxide nanoparticles from waste electric cables for electrochemical sensing. ACS Sustainable Chemical Engineering, 6(9), pp 12176–12186. DOI: 10.1021/acssuschemeng.8b02510.
4. Vanegas, D.C., L. Patiño, C. Mendez, D. Alves de Oliveira, A.M. Torres, E.S. McLamore, C. Gomes (2018). Low-Cost Electrochemical Biosensor for Detection of Biogenic Amines in Food Samples. Biosensors Journal, 8(2). DOI: 10.3390/bios8020042.
5. Hills, K.D., D. Alves De Oliveira, N. Cavallaro, C. Gomes, E.S. McLamore (2018). Actuation of chitosan-aptamer nanobrush borders as a mechanism for capturing pathogens. Analyst, 143: 1650-1661. DOI: 10.1039/c7an02039b.
6. Rong, Y., A.V. Pardon, K.J. Hagerty, N. Nelson, S. Chi, N.O. Keyhani, J. Katz, Shoumen Datta, C. Gomes, E.S. McLamore (2018). Post hoc support vector machine learning for biosensors based on weak protein-ligand interactions. Analyst, 143, 2066-2075. DOI: 10.1039/c8an00065d.
7. Vélez-Torres, I., D. Vanegas , E.S. McLamore, D. Hurtado (2018). Mercury Pollution and Artisanal Gold Mining in Alto Cauca, Colombia: Woman's Perception of Health and Environmental Impacts. Journal of Environment and Development, 27(4) 415–444. DOI: 10.1177/1070496518794796.
8. Bera, T., E.S. McLamore, B. Wasik, B. Rathinasabapathi, G. Liu (2018). Identification of a maize (Zea mays L.) inbred line adapted to low‐P conditions via analyses of phosphorus utilization, root acidification, and calcium influx. J. Plant Phys., 181(2): 275-286. DOI: 10.1002/jpln.201700319
9. Cannon, A.E., D.C. Vanegas, J. Wang, G. Clark, E.S. McLamore, S.J. Roux. Polarized Distribution of Extracellular Nucleotides Promotes Gravity-Directed Polarization of Development in Spores of Ceratopteris richardii. Plant Journal, In review
10. Boz, Z., Welt, B.A., Brecht, J.K., Pelletier, W., E.S. McLamore, G.A. Kiker, J.E Butler (2018). Review of challenges and advances in modification of food package headspace gases. Journal of Applied Packaging Research, 10(1): 62-67.

Station: Michigan State University

PI: Dr. Evangelyn Alocilja, Professor, Biosystems and Agricultural Engineering

Objective(s) Addressed: #3

Summary of Work:

1. Our research was Finalist for the 2017 Manufacturing Innovation Leadership Award (US national award)
2. One of students received the 2017 State of Michigan Stockholm Junior Water Prize Award; student represented the State of Michigan to the US Stockholm Junior Water Prize Competition
3. Technology transfer was our continuing activity. We received two US patents and filed several new invention disclosures while improving the technologies that are being reviewed by the US Patent and Trademark Office.
4. Our nano-biosensing technologies continue to be validated in clinical and biological samples (human, animal, and plant) for rapid disease and microbial-contaminant detection in our lab at MSU as well as with our collaborators around the world, such as in the Philippines, Nepal, India, Peru, Colombia, Mexico, and Poland.
5. Our antimicrobial films have been tested in various foods, such as lettuce, cucumber, strawberries, ground beef, salami, cheese, and chicken meat. Bacterial reduction ranges from 4 logs to 7 logs of bacterial contamination.
6. Our technology on nanoparticle-based anti-counterfeiting devices is continually featured in the Science of Innovation educational program by the National Science Foundation, US Patent and Trademark Office, and NBC Learn as a national resource to encourage and recruit K-12 students to the science fields. The video is entitled "Science of Innovation: Anti-Counterfeiting Devices" and can be viewed at www.nbclearn.com/innovation/cuecard/62970. This material will impact thousands of K-12 students and teachers not only in the US but also around the world.
7. My TEDMED talk on nano-biosensors continues to gain audiences from many sectors. The TED talk is featured in the following website: http://www.youtube.com/watch?v=QGauiO0Eev0.
8. Our publications and conference presentations allowed the dissemination of our research work to a broader group of researchers and potential users both in the US and around the world.
9. Technology transfer was our continuing activity. We received two US patents and filed several new invention disclosures while improving the technologies that are being reviewed by the US Patent and Trademark Office.
10. We have trained 16 undergraduate students, 3 PhD students, 2 high school students, and 1 high school teacher on nanotechnology and biosensors. The students won several awards. We have also trained scientists from the Philippines, Peru, Nepal, and India on the use of our technologies. These students and scientists will become the future research leaders in the emerging field of nano-biosensing for global health, biodefense, food safety, water quality, and product integrity.

Station: University of Missouri, Columbia, MO

PI: Mengshi Lin, Professor, Food Science Program, University of Missouri

Objective(s) Addressed: # 1, 2, & 3

Summary of Work:

Our objectives are to develop new technologies for characterizing fundamental nanoscale processes; construct and characterize self-assembled nanostructures; and develop devices and systems using nanotechnology.

In this reporting period, we developed an environmentally friendly and cost-effective approach to synthesize green silver nanoparticles (AgNPs) from silver precursors. Green synthesis of AgNPs was accomplished using the aqueous extract of turmeric powder, in which plant biomaterials were used as a reducing as well as a capping agent. After 24 h of reaction, the yellow color of extract was changed to dark brown-reddish due to the reduction of silver ions to AgNPs. AgNPs were characterized using UV-vis spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). The maximum absorbance of UV-vis spectra was at 432 nm. TEM analysis reveals that the shape of the most biosynthesized AgNPs was in spherical forms and the average of particle size was 18 nm. EDS analysis exhibits strong signals of silver element. In addition, green synthesized AgNPs show high and efficient antimicrobial activities against two food-borne pathogens (E. coli O157:H7 and L. monocytogenes). TEM and scanning electron microscopy images reveal that there were significant shrinkage and damage of bacterial cell wall, and the leakage or loss of bacterial intracellular contents. A significant reduction of bacterial counts just after 4 h of exposure was observed. These results indicate that green synthesized AgNPs can be utilized as an antimicrobial means to inhibit the growth of pathogenic bacteria for applications in agricultural and food industries.

Outputs:

1. Alsammarraie, A.K., Wang, W., Zhou, P., Mustapha, A., Lin, M. 2018. Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities. Colloids Surf. B Biointerfaces. 171, 398-405.
2. Liu, L., Kerr, W.L., Kong, F., Dee, D.R., Lin, M. 2018. Influence of nano-fibrillated cellulose (NFC) on starch digestion and glucose absorption. Polym. 196, 146-153.
3. Tian, K., Chen, X., Luan B., Singh, P., Yang, Z., Gates, K.S., Lin, M., Mustapha, A., Gu, L.-Q. 2018. Single LNA-enhanced genetic discrimination of foodborne pathogenic serotype in a nanopore. ACS nano. 12, 4194-4205.
4. Alsammarraie, A.K., Lin, M., Mustapha, A., Lin, H., Chen, X., Chen, Y., Wang, H., Huang, M. 2018.  Rapid determination of thiabendazole in juice by SERS coupled with novel gold nanosubstrates. Food Chem. 259, 219-225. 

Station: University of Wisconsin-Madison

PI: Sundaram Gunasekaran

Investigators/Participants: Sundaram Gunasekaran and Joel Pedersen

Objective(s) Addressed: # 1, 2, 3, & 4

Summary of Work:

Nanomaterials synthesis and biosensor fabrication for evaluating various analytes to ensure food (e.g., deleterious microbiological and chemical constituents) and environmental (e.g., heavy metals, prions) safety. Sustainable development of nanotechnology via molecular-level understanding of the interaction of nanomaterials with biological interfaces, both to design applications that interface with biological systems and to evaluate the potential risks posed by release of nanoscale materials into the environment.

Outputs:

1. Anu Prathap MU, Sadak O, Gunasekaran S. 2018. Rapid and scalable synthesis of ultrathin zeolitic imidazole framework (ZIF-8) and its use for the detection of trace levels of nitroaromatic explosives. Adv Sustainable Systems 2 (10),1800053
2. Xu X, Guo Y, Wang L, He K, Guo Y, Wang XQ, Gunasekaran S. 2018. Hapten-grafted programmed probe as corecognition element for competitive immunosensor to detect acetamiprid residue in agricultural products. J Ag and Fd Chem 66(29):7815-7821.
3. Sadak O, Sundramoorthy AK, Gunasekaran S. 2018. Facile and Green Synthesis of Highly Conductive Graphene. Carbon 138:108-117.
4. Urena-Saborio H, Alfaro-Viquez E, Esquivel-Alvarado D, Madrigal-Carballo S, Gunasekaran S. 2018. Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation. International J. of Biological Macromolecules 115:1218-1224.
5. You YS, Lim S, Hahn J, Choi YJ, Gunasekaran S. 2018. Bifunctional linker-based immunosensing for rapid and visible detection of bacteria in real matrices. Biosens Bioelectron 100:389-95
6. Xu XH, Guo YN, Wang XY, Li W, Qi PP, Wang Z, Gunasekaran S, Wang Q. 2018. Sensitive detection of pesticides by a highly luminescent metal-organic framework. Sensor Actuat B-Chem 260:339-45
7. Wang YC, Mohan CO, Guan JH, Ravishankar CN, Gunasekaran S. 2018. Chitosan and gold nanoparticles-based thermal history indicators and frozen indicators for perishable and temperature-sensitive products. Food Control 85:186-93
8. Melby, E. S.; Allen, C. R.; Foreman-Ortiz, I. U.; Caudill. E. R.; Kuech, T. R.; Vartanian, A. M.; Zhang, X.; Murphy, C. J.; Hernandez, R.; Pedersen, J. A. Peripheral membrane proteins dramatically alter nanoparticle interaction at lipid bilayer interfaces. Langmuir 2018, 34, 10793-10805.
9. Mensch, A. C.; Buchman, J. T.; Haynes, C. L.; Pedersen, J. A.; Hamers, R. J. Quaternary amine-terminated quantum dots induce structural changes to supported lipid bilayers. Langmuir 2018, 34.
10. Frank, B. P.; Durkin, D. P.; Caudill, E. R.; Zhu, L.; Curry, M. L.; Pedersen, J. A.; Fairbrother, D. H. Impact of silanization on the dispersion properties and biodegradability of nanocellulose. ACS Appl. Nano Mater. 2018, 1.
11. Olenick, L. L.; Troiano, J. M.; Vartanian, A.; Melby, E. S.; Mensch, A. C.; Zhang, L.; Hong, J.; Mesele, O.; Qiu, T.; Bozich, J.; Lohse, S.; Zhang, X.; Kuech, T. R.; Millevolte, A.; Gunsolus, I.; McGeachy, A. C.; Doğangün, M.; Li, T.; Hu, D.; Walter, S. R.; Mohaimani, A.; Schmoldt, A.; Torelli, M. D.; Hurley, K. R.; Dalluge, J.; Chong, G.; Feng, Z. V.; Haynes, C. L.; Hamers, R. J.; Pedersen, J. A.; Cui, Q.; Hernandez, R.; Klaper, R.; Orr, G.; Murphy, C. J.; Geiger, F. M. Lipid corona formation from nanoparticle interactions with bilayers and membrane-specific biological outcomes. Chem 2018, 4, 2709-2723.
12. Plummer, I. H.; Johnson, C. J.; Chesney, A. R.; Pedersen, J. A.; Samuel, M. D. Mineral licks as environmental reservoirs for chronic wasting disease prions. PLoS ONE 2018, 13, e0196745.

Planned Activities:

The team has identified the following subgroups with their associated participants to facilitate discussion and collaboration among the members.

1. Inline measurement processing
   1. Chenxu Yu*
   2. Mingshi Lin
   3. Yanbin Li
   4. Ramaraja Ramasamy
   5. Paul Takhistov
2. Matrix challenge
   1. Ramaraja Ramasamy
   2. Evangelyn Alocilja*
   3. Paul Takhistov
   4. Sundaram Gunasekaran
   5. Margaret Frey
3. False +/- (in general)
   1. Ramaraja Ramasamy*
   2. Sundaram Gunasekaran
   3. Evangelyn Alocilja
4. Dead vs. Live cells
   1. Ramaraja Ramasamy
   2. Evangelyn Alocilja
   3. Jeremy Tzeng*
   4. Olga Tsyusko

1. Cao, L.L., Q. Zhang, H. Dai, Y.C. Fu, and Li. 2018. Separation/concentration-signal-amplification in-one method based on electrochemical conversion of magnetic nanoparticles for electrochemical biosensing. Electroanalysis 30(3):517-524. DOI: 10.1002/elan.201700653
2. Dai, H., Y.Q. Li, Y.C. Fu, and Li. 2018. Enzyme catalysis induced polymer growth in nanochannels: A new approach to regulate ion transport and to study enzyme kinetics in nanospace. Electroanalysis 30(2):328-335 (available online Dec. 18, 2017). DOI:10.1002/elan.201700703
3. Dai, H., Y.Q. Li, Q. Zhang, Y.C. Fu, and Li. 2018. A colorimetric biosensor based on enzyme-catalysis-induced production of inorganic nanoparticles for sensitive detection of glucose in white grape wine. RSC Advances 8:33960-33967. DOI: 10.1039/c8ra06347h
4. Hu, Q.Q., R.H. Wang, H. Wang, M.F. Slavik and Li. 2018. Selection of acrylamide-specific aptamers by a quartz crystal microbalance combined SELEX method and their application in rapid and specific detection of acrylamide. Sensors and Actuators: B: Chemical 273:220-227. doi.org/10.1016/j.snb.2018.06.033
5. Li, Z.S., G.S. Zhou, H. Dai, M.Y. Yang, Y.C. Fu, Y.B. Ying, and Li. 2018. Biomineralization-mimetic preparation of hybrid membranes with ultra-high load of pristine metal-organic frameworks grew on silk nanofibers for hazards collection in water. Journal of Materials Chemistry A 6(8):3402-3413 (published online on December 5, 2017). DOI:10.1039/C7TA06924C
6. Wang, H., L.J. Wang, Q.Q. Hu, R.H. Wang, Li and M. Kidd. 2018. Rapid and sensitive detection of Campylobacter jejuni in poultry products using a nanoparticles-based piezoelectric immunosensor integrated with magnetic immunoseparation. Journal of Food Protection 81(8):1321-1330. doi:10.4315/0362-028X.JFP-17-381
7. Wang, L.J., R.H. Wang, H. Wei, and Li. 2018. Selection of aptamers against pathogenic bacteria and their diagnostics application. World Journal of Microbiology and Biotechnology 34:149. doi.org/ 10.1007/s11274-018-2528-2
8. Xu, C.N., L.Y. Lan, Y. Yao, J.F. Ping, Li, and Y.B. Ying. 2017. An unmodified gold nanorods-based DNA colorimetric biosensor with enzyme-free hybridization chain reaction amplification. Sensors & Actuators: B. Chemical. 273:642-648. doi.org/:10.1016/j.snb.2018.06.035
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