SAES-422 Multistate Research Activity Accomplishments Report

Sections

Basic Information
Additional Info

Status: Approved

Basic Information

Project No. and Title: NE1701 : Mycobacterial Diseases of Animals
Period Covered: 12/01/2017 to 09/30/2018
Date of Report: 03/01/2018
Annual Meeting Dates: 12/03/2017 to 12/05/2017

Bannantine, John - NADC-USDA-ARS Barletta, Raul - University of Nebraska–Lincoln Bermudez, luiz - Oregon State Chang, Yung Fu - Cornell University Coussens, Paul - Michigan State University Gerdts, Volker - VIDO-InterVac Gibbons-Bergener, Suzanne - Wisconsin Department of Public Health Grohn, Yrjo - Cornell University Johnson, Peter - USDA-NIFA Kapur, Vivek - Penn State Katani, Robab - Penn State Kerhli, Marcus - USDA-ARS/Iowa State University Morrow, Alex - Centre for Agriculture and Biosciences International, UK Olea-Popelka, Francisco - Colorado State University Olson, Ken - AAMD Patton, Elizabeth - Wisconsin Department of Ag Quinn, Fred - Univ. of Georgia Robbe-Austerman, Suelee - USDA-APHIS-NVSL Smith, Rebecca - Cornell University Sreevatsan, Srinand - Michigan State Univ. Talaat, Adel - Univ. Wisconsin Wagner, Bettina - Cornell University

Brief Summary of Minutes of Annual Meeting

Attached file:

NE1707-Meeting-Minutes.pdf

Accomplishments

Investigators have made considerable progress in the following areas:

Dr. Wagner’s research group has established a new multiplex assay platform for serological differentiation of Johne’s disease stages. Comparison of individual MAP antigen-based assays indicated better differentiation of samples in the low positive range, improved analytical sensitivity, earlier detection of MAP infection especially with some antigens, and improved sensitivity and specificity even at individual assay level.

Dr. Sreevatsan’s group focuses initially on the evaluation of pathogen-specific biomarkers for the diagnosis of tuberculosis and the objective of his research is on developing a noninvasive biomarker-based detection system specific for Mycobacterium bovis for monitoring infection in animals and humans. He uses the pathogen peptide as a biomarker as it is a MTC-specific biomarker, can differentiate between different stages of the disease, and it has specificity and sensitivity in low/high disease prevalence areas. His research team use the iTRAQ - isobaric Tags for Relative Quantification to determine the amount of proteins from different sources in a single experiment.

Dr. Grohn’s research is to focus on the increase understanding of the epidemiology and transmission of Mycobacterial diseases including predictive modeling. The approach of this research is on developing a quantitative methodology for incorporating whole genome sequence data into bacterial transmission models for infectious diseases incorporating ecology, economics, molecular biology, and epidemiology, and to apply these methods and models towards better understanding the principles and dynamics governing transmission of mycobacterial infection.

Dr. Barletta’s research focuses on the MAP K-10 MycomarT7 mutant library generation. A transposon insertion “hot spot” downstream from the MAP_4116c (mmaA4) gene resulted in a biased identification of essential genes.

Dr. Bermudez’s research group focused on the M.bovis interaction with mucosal cells, the bacterial surface expression, and the adaptive (Ig)Immune Response. Dr. Bermudez also presented some freeze-fracture Transmission electron microscopy of M.bovis infected bovine macrophages.

Dr. SueLee Robbe-Austerman’s effort has concluded that 95% of human cases in Baja CA and Southern CA originate from cows in the Baja CA non-accredited zone. Additionally, the study suggested that there are unique events that cause most of the spill over into humans, and little evidence of human to human transmission was determined. The data also suggested that possible human to cow transmission may have occurred.

Dr. Talaat’s group works on live attenuated pathogens missing virulence or metabolism genes, by design or through passaging, maintain a balance between attenuation and virulence (M. tb vs. BCG), vaccine strain selection, and rational vaccine design, based on mechanistic studies.

Dr. Patton’s group has refined testing protocols to including, communication/action checklist, animal worker questionnaire to identify elevated-risk tasks, animal risk assessment tool, inclusion of occupational health programs for abattoir workers, expanded interagency zoonotic disease communication plan, and targeted TB screening and testing programs for animal workers.

Extension is led by Dr. Ken Olson, as they continue to collaborate with the VBJDCP to address education, share information, and provide tools for producers, other scientists, industry partners, and USDA. Dr. Olson also attends DC meetings with USDA leadership (APHIS, NIFA, ARS,), partner organizations (AVMA, IDFA, NMPF, NASDA, AFBF), and Congressional staff. Furthermore, his efforts concentrate on the awareness of the MDA MI among stakeholders, industry and government leaders.

Impacts

Application of an established mathematical model of infection in data collection from the longitudinal study done in three states (NY, PA, VT) for approximately 10 years.
Implementation of a faster test-and-removal plan with a 2-month testing interval in epidemic disease control of the bTB in cattle herds.
Establishment of a Bayesian computation modeling and simulation approach to estimate the effectiveness of vaccination against infectious diseases in food animal populations.
Comparison and evaluation of the fully sequenced two USA virulent M. bovis isolates.
Publication of close to 30 mycobacterium-related manuscripts.
Vaccine development testing using Live Attenuated Vaccine and Reverse Vaccinology Approach for the Prevention of Mycobacterial Disease in Cattle (ReVAMP).
Establishment of a new multiplex assay platform for serological differentiation of Johne’s disease stages.
Establishment of genomic, SNP analysis and bioinformatics related to mycobacterial diseases.

Publications

Li L, Wagner B, Freer H, Schilling M, Bannantine JP, Campo JJ, Katani R, Grohn YT, Radzio-Basu J, Kapur V. Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays. PLoS One. 2017 Dec 19;12(12):e0189783. doi: 10.1371/journal.pone.0189783. eCollection 2017. PubMed PMID: 29261761; PubMed Central PMCID: PMC5736219.

Rathnaiah G, Zinniel DK, Bannantine JP, Stabel JR, Gröhn YT, Collins MT, Barletta RG. Pathogenesis, Molecular Genetics, and Genomics of Mycobacterium avium subsp. paratuberculosis, the Etiologic Agent of Johne's Disease. Front Vet Sci. 2017 Nov 6;4:187. doi: 10.3389/fvets.2017.00187. eCollection 2017. Review. PubMed PMID: 29164142; PubMed Central PMCID: PMC5681481.

Li L, Bannantine JP, Campo JJ, Randall A, Grohn YT, Katani R, Schilling M, Radzio-Basu J, Kapur V. Identification of sero-reactive antigens for the early diagnosis of Johne's disease in cattle. PLoS One. 2017 Sep 1;12(9):e0184373. doi: 10.1371/journal.pone.0184373. eCollection 2017. PubMed PMID: 28863177; PubMed Central PMCID: PMC5581170.

Grohn YT, Carson C, Lanzas C, Pullum L, Stanhope M, Volkova V. A proposed analytic framework for determining the impact of an antimicrobial resistance intervention. Anim Health Res Rev. 2017 Jun;18(1):1-25. doi: 10.1017/S1466252317000019. Epub 2017 May 16. Review. PubMed PMID: 28506325.

Dong H, Lv Y, Sreevatsan S, Zhao D, Zhou X. Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model. PLoS One. 2017 Aug 24;12(8):e0183666. doi: 10.1371/journal.pone.0183666. eCollection 2017. PubMed PMID: 28837698; PubMed Central PMCID: PMC5570376.

Singhla T, Boonyayatra S, Punyapornwithaya V, VanderWaal KL, Alvarez J, Sreevatsan S, Phornwisetsirikun S, Sankwan J, Srijun M, Wells SJ. Factors Affecting Herd Status for Bovine Tuberculosis in Dairy Cattle in Northern Thailand. Vet Med Int. 2017;2017:2964389. doi: 10.1155/2017/2964389. Epub 2017 May 3. PubMed PMID: 28553557; PubMed Central PMCID: PMC5434264.

Chunfa L, Xin S, Qiang L, Sreevatsan S, Yang L, Zhao D, Zhou X. The Central Role of IFI204 in IFN-β Release and Autophagy Activation during Mycobacterium bovis Infection. Front Cell Infect Microbiol. 2017 May 5;7:169. doi: 10.3389/fcimb.2017.00169. eCollection 2017. PubMed PMID: 28529930; PubMed Central PMCID: PMC5418236.

Bannantine JP, Campo JJ, Li L, Randall A, Pablo J, Praul CA, Raygoza Garay JA, Stabel JR, Kapur V. Identification of Novel Seroreactive Antigens in Johne's Disease Cattle by Using the Mycobacterium tuberculosis Protein Array. Clin Vaccine Immunol. 2017 Jul 5;24(7). pii: e00081-17. doi: 10.1128/CVI.00081-17. Print 2017 Jul. PubMed PMID: 28515134; PubMed Central PMCID: PMC5498720.

Shippy DC, Lemke JJ, Berry A, Nelson K, Hines ME 2nd, Talaat AM. Superior Protection from Live-Attenuated Vaccines Directed against Johne's Disease. Clin Vaccine Immunol. 2017 Jan 5;24(1). pii: e00478-16. doi: 10.1128/CVI.00478-16. Print 2017 Jan. PubMed PMID: 27806993; PubMed Central PMCID: PMC5216426.

Grant IR, Foddai ACG, Tarrant JC, Kunkel B, Hartmann FA, McGuirk S, Hansen C, Talaat AM, Collins MT. Viable Mycobacterium avium ssp. paratuberculosis isolated from calf milk replacer. J Dairy Sci. 2017 Dec;100(12):9723-9735. doi: 10.3168/jds.2017-13154. Epub 2017 Oct 4. PubMed PMID: 28987590.

Sandoval-Azuara SE, Muñiz-Salazar R, Perea-Jacobo R, Robbe-Austerman S, Perera-Ortiz A, López-Valencia G, Bravo DM, Sanchez-Flores A, Miranda-Guzmán D, Flores-López CA, Zenteno-Cuevas R, Laniado-Laborín R, de la Cruz FL, Stuber TP. Whole genome sequencing of Mycobacterium bovis to obtain molecular fingerprints in human and cattle isolates from Baja California, Mexico. Int J Infect Dis. 2017 Oct;63:48-56. doi: 10.1016/j.ijid.2017.07.012. Epub 2017 Jul 22. PubMed PMID: 28739421.

Waters WR, Vordermeier HM, Rhodes S, Khatri B, Palmer MV, Maggioli MF, Thacker TC, Nelson JT, Thomsen BV, Robbe-Austerman S, Bravo Garcia DM, Schoenbaum MA, Camacho MS, Ray JS, Esfandiari J, Lambotte P, Greenwald R, Grandison A, Sikar-Gang A, Lyashchenko KP. Potential for rapid antibody detection to identify tuberculous cattle with non-reactive tuberculin skin test results. BMC Vet Res. 2017 Jun 7;13(1):164. doi: 10.1186/s12917-017-1085-5. PubMed PMID: 28592322; PubMed Central PMCID: PMC5463416.

Malone KM, Farrell D, Stuber TP, Schubert OT, Aebersold R, Robbe-Austerman S, Gordon SV. Updated Reference Genome Sequence and Annotation of Mycobacterium bovis AF2122/97. Genome Announc. 2017 Apr 6;5(14). pii: e00157-17. doi: 10.1128/genomeA.00157-17. PubMed PMID: 28385856; PubMed Central PMCID: PMC5383904.

Danelishvili L, Chinison JJJ, Pham T, Gupta R, Bermudez LE. The Voltage-Dependent Anion Channels (VDAC) of Mycobacterium avium phagosome are associated with bacterial survival and lipid export in macrophages. Sci Rep. 2017 Aug 1;7(1):7007. doi: 10.1038/s41598-017-06700-3. PubMed PMID: 28765557; PubMed Central PMCID: PMC5539096.

Jeffrey B, Rose SJ, Gilbert K, Lewis M, Bermudez LE. Comparative analysis of the genomes of clinical isolates of Mycobacterium avium subsp. hominissuis regarding virulence-related genes. J Med Microbiol. 2017 Jul;66(7):1063-1075. doi: 10.1099/jmm.0.000507. Epub 2017 Jul 3. PubMed PMID: 28671535.

Danelishvili L, Shulzhenko N, Chinison JJJ, Babrak L, Hu J, Morgun A, Burrows G, Bermudez LE. Mycobacterium tuberculosis Proteome Response to Antituberculosis Compounds Reveals Metabolic "Escape" Pathways That Prolong Bacterial Survival. Antimicrob Agents Chemother. 2017 Jun 27;61(7). pii: e00430-17. doi: 10.1128/AAC.00430-17. Print 2017 Jul. PubMed PMID: 28416555; PubMed Central PMCID: PMC5487666.

Bannantine JP, Etienne G, Laval F, Stabel JR, Lemassu A, Daffé M, Bayles DO, Ganneau C, Bonhomme F, Branger M, Cochard T, Bay S, Biet F. Cell wall peptidolipids of Mycobacterium avium: from genetic prediction to exact structure of a nonribosomal peptide. Mol Microbiol. 2017 Aug;105(4):525-539. doi: 10.1111/mmi.13717. Epub 2017 Jun 15. PubMed PMID: 28558126.

Park KT, Elnaggar MM, Abdellrazeq GS, Bannantine JP, Mack V, Fry LM, Davis WC. Correction: Phenotype and Function of CD209+ Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages. PLoS One. 2017 Jan 25;12(1):e0171059. doi: 10.1371/journal.pone.0171059. eCollection 2017. PubMed PMID: 28122067; PubMed Central PMCID: PMC5266315.

Souza CD, Bannantine JP, Brown WC, Norton MG, Davis WC, Hwang JK, Ziaei P, Abdellrazeq GS, Eren MV, Deringer JR, Laws E, Cardieri MCD. A nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid A and recombinant Mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages. J Appl Microbiol. 2017 May 14. doi: 10.1111/jam.13491. [Epub ahead of print] PubMed PMID: 28502107.

Venegas-Vargas C, Manning SD, Coussens PM, Roussey JA, Bartlett P, Grooms D. Bovine Leukemia Virus and Mycobacterium avium subsp. paratuberculosis Are Not Associated with Shiga Toxin-Producing Escherichia coli Shedding in Cattle. J Food Prot. 2017 Jan;80(1):86-89. doi: 10.4315/0362-028X.JFP-16-090. PubMed PMID: 28221870.

Frie MC, Sporer KRB, Kirkpatrick BW, Coussens PM. T and B cell activation profiles from cows with and without Johne's disease in response to in vitro stimulation with Mycobacterium avium subspecies paratuberculosis. Vet Immunol Immunopathol. 2017 Dec;193-194:50-56. doi: 10.1016/j.vetimm.2017.10.005. Epub 2017 Nov 7. PubMed PMID: 29129227.

Ashraf A, Imran M, Yaqub T, Tayyab M, Shehzad W, Mingala CN, Chang YF. Development and validation of a loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis in mastitic milk. Folia Microbiol (Praha). 2017 Dec 14. doi: 10.1007/s12223-017-0576-x. [Epub ahead of print] PubMed PMID: 29243178.

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