Brief Summary of Minutes of Annual Meeting

On Thursday, June 15, welcome and introduction was given by institutional co-host, Dr. Tim Safranski.  Dr. Bill Lamberson, Interim Chair of the Division of Animal Science, gave an overview of the University of Missouri – College of Agriculture, Food and Natural Resources (CAFNR), of which, approximately 500 undergraduate and 60 graduate students are within the Animal Science curriculum.  Current NCERA57 Chair, Dr. Lea Rempel, USMARC, opened the annual meeting portion with greetings and appreciation to University of Missouri, the local hosts, Drs. Tim Safranski and Rod Geissert for their generous hospitality and coordination of the meeting facility.  Station reports from several institutions were given.

Reported peer –reviewed publications from cooperating institutions within the NCERA-57 group yielded 49 manuscripts, abstracts, proceeding and book chapters associated with swine reproduction, physiology or genetics.

Boar Performance (IN, NE, IL)

A. Proctor, D. W. Lugar, M. C. Lucy, T. J. Safranski and K. R. Stewart. 2017. Effects of in Utero Heat Stress on Boar Growth and Reproduction Prior to, during, and after Puberty. Midwest ASAS, Omaha, NE. Abstract.

W. Lugar, W. A. Krom, J. A. Proctor, P. D. Mings, and K. R.Stewart. 2017. Effects of Supplemental Betaine to Semen Extenders on Semen Quality in Boars. Midwest ASAS, Omaha, NE. Abstract.

Lents, C.L., J.F. Thorson, A.T. Desaulniers and B.R. White.  2017.  RFamide-related peptide 3 and gonadotropin-releasing hormone-II are autocrine-paracrine regulators of testicular function in the boar.  Mol. Reprod. Dev.  May 5.  doi: 10.1002/mrd.22830.  [Epub ahead of print] Review.

Gonzalez-Pena D, Knox RV, Rodriguez-Zas SL. 2016. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study. Theriogenology. 85:335-44.

Silva E, Frost D, Li L, Bovin N, Miller DJ. 2017. Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine sperm. Andrology doi: 10.1111/andr.12340

Winters RA, Nettenstrom LM, Lopez DG, Willenburg KL, Vishwanath R, Miller DJ. 2017. Effect of sorting porcine spermatozoa by sex chromosomes on oviduct cell binding. Submitted.

Sow and Gilt Performance (IN, NE, IL, KS, USMARC)

 Cabezon, F. K.R. Stewart, A.P. Schinckel, B.T. Richert, M. Gandarillas, M. Pasache, and W.A. Peralta.  2016.  Effect of betaine supplementation during summer on sow lactation and subsequent farrowing performance. Professional Animal Scientist, 32(5), pp 698-706.

Cabezón, F.A., Schinckel, A.P., Richert, B.T., Stewart, K.R., Gandarillas, M. and Peralta, W.A., 2016. Analysis of lactation feed intakes for sows including data on environmental temperatures and humidity. The Professional Animal Scientist, 32(3), pp.333-345.

N.M. Chapel, C.J. Byrd, D. W. Lugar, K. R. Stewart, T.J. Safranski, L.H. Baumgard, and J.S. Johnson.  2017.  The effects of in utero heat stress on fasting heat production in growing pigs.  Midwest ASAS, Omaha, NE. Abstract.

Thorson, J.F., N.L. Heidorn, V. Ryu, K. Czaja, D.J. Nonneman, C.R. Barb, G.J. Hausman, G.A. Rohrer, L.D. Prezotto, R.B. McCosh, E.C. Wright, B.R. White, B.A. Freking, W.T. Oliver, S.M. Hileman and C.A. Lents.  2017.  Neuropeptide FF receptor function affects gonadotropin secretion and age at puberty in gilts.  Biol. Reprod.  96:617-634.

Knox RV, Esparza-Harris KC, Johnston ME, Webel SK. 2017. Effect of numbers of sperm and timing of a single, post-cervical insemination on the fertility of weaned sows treated with OvuGel®. Theriogenology 92:197-203.

Knox RV, Shen J, Greiner LL, Connor JF. 2016. Effect of timing of relocation of replacement gilts from group pens to individual stalls before breeding on fertility and well-being. J. Anim. Sci. 94:5114-5121.

Knox RV. Artificial insemination in pigs today. Theriogenology. 2016.85: 83-93.

Arend L, Knox RV, Greiner L, Graham A, Connor J.  2016.  Effects of feeding melatonin during proestrus and early gestation in gilts and P1 sows to minimize the effects of seasonal infertility. Midwest ASAS/ADSA Annual Meeting.

Arend LS, Duangkamol P, Knox RV. 2016. Administration of melatonin during the follicular and early luteal phase to mimic short days and minimize seasonal infertility for prepubertal gilts housed under differing hours of light and heat. Allen D. Leman Swine Conf. St. Paul, MN.

Vaughn, M., C. Serrano, D. Burnett, D. Davis, J. Woodworth, and J. Gonzalez.  2016. Influence of Porcine Plasma Supplementation on Gestating Sow Serum IGF-1 Concentration and Litter Weights. Professional Agricultural Workers Journal. 4: No. 1,

L. Frobose, M. D. Tokach, J. M. DeRouchey, S. S. Dritz, R. D. Goodband, J. L. Nelssen, and D. L. Davis. 2017. Follicle development, incidence of lactational estrus, and ovulation in sows exposed to different suckling reduction strategies. International Conference on Pig Reproduction. June 11-14, Columbia, MO.

J.M. Morton, A.J. Langemeier, T. Rathbun, D.L. 2017. Split suckling and birth weight affects colostrum intake and pre-weaning weight gain. International Conference on Pig Reproduction. June 11-14, Columbia, MO.

Vallet JL, Meyer S. 2016. Effect of glucosamine supplementation on litter size in a commercial setting - NPB #14-238. National Pork Board.

Vallet, J.L., Miles, J.R.  2017. The effect of farrowing induction on colostrum and piglet serum immunocrits is dependent on parity.  Journal of Animal Science 96(2): 688-696.  doi:10.2527/jas.2016.0993.

Growth, Development, Physiology (NE, IL, USMARC)

Riccio G, Ellerbrock R, Canisso I, Knox R, Kline K. 2016. Motility of stallion spermatozoa after centrifugation and cooling in INRA96® or Walworth Extender. Journal of Agricultural Science and Technology. A 6 143-147.

Silva E, Miller DJ. 2016. Involvement of specific oviduct cell glycans in sperm binding to form a reservoir. J Vet Andrology 1:24-28.

Phoophitphong D., Tummaruk P, Knox RV. 2016. Serum estradiol-17B concentration after estrus induction using PG600 in gilts. 24^th International Pig Veterinary Society Congress. P0-PW1-247.

Tummaruk T, Phoophitphong D, Knox RV. Follicle development, ovulation, and evidence of silent heat in gilts after estrus induction using gonadotrophin under tropical climate. 24^th International Pig Veterinary Society. Dublin. 2016. PO-PW1-239.

Lents CA, Brown-Brandl TM, Rohrer GA, Oliver WT, Freking BA. 2016. Plasma concentrations of acyl-ghrelin are associated with average daily gain and feeding behavior in grow-finish pigs. Domestic Animal Endocrinology. 55:107-113.

Rempel LA, Miles JR, Parrish J. 2016. Evaluation of contributions to seasonal reproductive inefficiency; NPB #14-052. National Pork Board. Available: http://research.pork.org/FileLibrary/ResearchDocuments/14-052-REMPEL-final%20rpt.pdf.

Thorson, J., Heidorn, N.L., Ryu, V., Czaja, K., Nonneman, D.J., Barb, C.R., Hausman, G.J., Rohrer, G.A., Prezotto, L.D., McCosh, R.B., Wright, E.C., White, B.R., Freking, B.A., Oliver, W.T., Hileman, S.M., Lents, C.A. 2017.  Relationship of neuropeptide FF receptors with pubertal maturation of gilts. Biological Reproduction 96(3):617-634.  doi:10.1095/biolreprod.116.144998.

Basic Science and Technology (IN, KS, NE, IL)

Herrick JR, Wang C, Machaty Z. 2016. The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes. Reprod Fertil Dev 28:599-607.

Machaty Z. 2016. Signal transduction in mammalian oocytes during fertilization. Cell Tissue Res 363:169-183.

Silva PV, Zhang L, Jaeger LA, Machaty Z. 2016. Effects of ORAI1 down-regulation on oocyte maturation in pigs. Proc 18^th International Congress on Animal Reproduction. Tours, France, p.280.

Zhang L, Chao C-H, Jaeger LA, Machaty Z. 2016. Repetitive interactions between STIM1 and ORAI1 at fertilization in pig oocytes. Proc 18^th International Congress on Animal Reproduction. Tours, France, p. 50.

Machaty Z, Miller AR, Zhang L. 2017. Egg activation at fertilization. In: “Vertebrate development”. (Eds.: F. Pelegri, M. Danilchik, A. Sutherland). Adv. Exp. Med. Biol., Vol. 953. pp. 1-47. Springer, Cham, Switzerland.

Zhang L, Machaty Z. 2017. Targeted gene knockdown in early embryos using siRNA. In: “Zygotic genome activation”. (Ed.: K. Lee). Methods Mol Biol., pp. 207-217. Humana Press, New York, NY, USA.

Morton, T. Rathbun and D.L. Davis. 2016. Relationships between pig weights at birth and weaning and expression of EGR1, Pref1, Cox1 and Cox2 in Wharton’s jelly and epididymal fat pad. Proc. Perinatal Biology Symposium: Interconnecting Animal and Human Systems to Understand Life-Disease.

Brauer, V.M., J.R. Wiarda Bell, A.T. Desaulniers, R.A. Cederberg and B.R. White.  2016.  Functional activity of the Gnrhr2 gene promoter in testis-derived cells is partially conferred by nuclear factor-kB, specificity protein 1 and 3 (SP1/3) and overlapping early growth response 1/SP1/3 binding sites.  Gene 587:137-146.

Xie, F., R.L. Krisher and J.R. Wood.  2016.  Oxidative stress during oocyte in vitro maturation increases the abundance of Dppa3 and Pou5f1 maternal effect gene transcripts in matured oocytes and two-cell embryos, indicative of altered post-transcriptional regulation of maternal mRNAs.  Society for the Study of Reproduction 48th Annual Meeting, San Diego, CA. p. 344.

Daigneault B, Miller DJ.  2017. Transient receptor potential cation channel subfamily P member 2 (TRPP2) regulates motility and intracellular calcium of porcine sperm. Submitted.

Sharif M, Silva E, Shah ST, Miller DJ. 2017. Redistribution of soluble N-ethylmaleimide-sensitive-factor attachment protein receptors in mouse sperm membranes prior to the acrosome reaction. Biol Reprod 96:352-365. doi: 10.1095/biolreprod.116.143735, PMID: 28203732

Daigneault BW and DJ Miller. 2016. TRPP2 regulates motility and intracellular calcium of porcine sperm. 42^nd IETS Annual Conference.

Dutta S and DJ Miller. 2016. Oviduct glycans prolong the lifespan of bovine sperm. 49^th Annual Meeting of the Society for the Study of Reproduction.

Elsokary MM and DJ Miller.  2017.  Mature oocytes trigger the release of bovine sperm from an immobilized oviduct glycan.  43^rd IETS Annual Conference. Austin, TX  Reprod Fertil Dev

Elsokary MM, Miller DJ.  2017.  Mature cumulus-oocyte complexes trigger the release of bovine sperm from an immobilized oviduct glycan.  50^th Annual Meeting of the Society for the Study of Reproduction.

Lotti S, Rubessa M, Knox RV, Wheeler MB. 2017. Effects of liposomes on sperm motility and DNA binding efficiency. International Embryo Transfer Society.

Machado SA, Miller DJ. 2017. The release of porcine sperm from oviduct cells is induced by progesterone and requires CatSper. 50^th Annual Meeting of the Society for the Study of Reproduction.

Machado SA, Sharif M, Kadirvel G, Bovin N, Miller DJ. 2017. Oviduct glycans regulate sperm Ca²⁺ influx and sperm viability. 50^th Annual Meeting of the Society for the Study of Reproduction.

Sharif M, Kerns K, Sutovsky P, Miller DJ.  2017.  Proteasomal activity is necessary for porcine sperm release from immobilized oviduct glycans. 50^th Annual Meeting of the Society for the Study of Reproduction.

Winters R and Miller DJ. 2016. Effect of sorting boar spermatozoa by sex chromosomes on oviduct cell binding.  49^th Annual Meeting of the Society for the Study of Reproduction.

Winters RA, Hamilton DN, Bhatnagar AS, Fitzgerald R, Bovin N, Miller DJ. 2017. Porcine sperm binding to oviduct cells and their glycans as supplements to laboratory semen analysis. 50^th Annual Meeting of the Society for the Study of Reproduction.

Genetics and Genomics (NE, USMARC)

Desaulniers, A.T., R.A. Cederberg, G.A. Mills, C.A. Lents and B.R. White.  2017.  Production of a gonadotropin-releasing hormone 2 receptor knockdown (GnRHR2 KD) swine line.  Transgenic Res.  May 22.  doi: 10.1007/s11248-017-0023-4.  [Epub ahead of print].

McDonald, E.A., J.E. Smith, R. A. Cederberg and B.R. White.  2016.  Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites.  Reprod. Biol. Endocrinol. 14:36.

Cagnone G, Tsai T-S, Srirattana K, Rossello F, Powell DR, Rohrer G, Cree L, Trounce IA, St John JC. 2016. Segregation of naturally occurring mitochondrial DNA variants in a mini-pig model. Genetics. 202(3):931-944.

Desaulniers AT, Cederberg RA, Lents CA, White BR. 2016. Use of genetically engineered swine to elucidate testis function in the boar. Large Animal Genetic Engineering Summit. September 18-20 2016, Bethesda, Maryland, p. 15-16, Symposium Proceedings. (2pp).

Freking BA, Lents CA, Vallet JL. 2016. Selection for uterine capacity improves lifetime productivity of sows. Animal Reproduction Science. 167:16-21.

Nonneman, D.J., Lents, C.A., Keel, B.N., Rohrer, G.A.  2017.  USMARC update on swine reproduction research.  In proceedings: Plant and Animal Genome Conference XXIV. Jan. 14-18, 2017. San Diego, CA. W914.

Rohrer, G.A., Cross, A.J., Lents, C.A., Miles, J.R., Nonneman, D.J., Rempel, L.A. 2017.  Genetic improvement of sow lifetime productivity. [abstract]  Journal of Animal Science 95(Suppl 2), p.11-12.

Rohrer, G.A., Nonneman, D.J.  2017.  Genetic analysis of teat number in pigs reveals some developmental pathways independent of vertebra number and several loci which only affect a specific side.  Genetics Selection Evolution 49:4.  doi:10.1186/s12711-016-0282-1.

White, B.R., Desaulniers, A.T., Cederberg, R.A., Mills, G.A., Lents, C.A. 2017.  A transgenic boar model to elucidate the role of gonadotropin-releasing hormone 2 (GnRH2) and its receptor in regulating testes and sperm function.  Journal of Animal Science 95(Suppl 2) p. 150.  doi:10.2527/asasmw.2017.308.

Wijesena, H.R., Lents, C.A., Reithoven, J., Trenhaile-Grannemann, M.D., Thorson, J.F., Keel, B.N., Miller, P.S., Spangler, M.L., Kachman, S.D., Ciobanu, D.C.  2017.  Integration of genomic approaches to uncover sources of variation in age at puberty and reproductive longevity in sows.  Journal of Animal Science 95 (Suppl 2) p.12.  doi:10.252

Teaching and Extension (NE, IL)

Desaulniers, A.T., R.M. McFee and B.R. White.  2016.  Smoothing the transition from classroom to hog farm:  Use of case studies to enhance swine industry knowledge and interest in college students.  Nebraska Pork Talk, Nebraska Pork Producers Association. 48:14-15.

Desaulniers, A.T., R.M. McFee and B.R. White.  2016.  Case-based reasoning enhances learning in an undergraduate reproductive biology course.  Society for the Study of Reproduction 48th Annual Meeting, San Diego, CA. p. 199.

Gonzalez-Pena D, Knox RV, Rodriguez-Zas SL. 2016. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study. Theriogenology. 85:335-44.