SAES-422 Multistate Research Activity Accomplishments Report

Sections

Status: Approved

Basic Information

Participants

1. Kristen Govoni University of Connecticut 2. Milan Shipka University of Alaska 3. Andrea Cupp University of Nebraska 4. Deb Hamernik University of Nebraska 5. Ryan Ashley New Mexico State 6. Caleb Lemley Mississippi State University 7. Mark Mirando USDA-NIFA 8. Brenda Alexander University of Wyoming 9. Phil Cardoso University of Illinois 10. Kim Vonnahme North Dakota State University 11. Larry Reynolds North Dakota State University 12. Anna Grazul-Bilska North Dakota State University 13. Ligia Prezetto Montana State University 14. Ky G. Pohler University of Tennessee 15. Anna Denico Cal Davis 16. Ron Randel Texas A&M 17. Russ Anthony Colorado State University 18. David Grieger Kansas State University

Phil Cardoso (chair) called to order at 1:10 PM.

State updates and introductions:

      1:10 PM introductions.

In 2018 the meeting will be in Overton, TX on 5/22-23.

Ligia Prezetto was elected the new member at large.

Update from Milan: W-3112 is the recipient of the Western Section Multistate group. The group will compete for the national award.

For next year, keep it to 3 slides to be presented in 10 min.

State reports of accomplishments

5:00 PM Meeting adjourned for Day 1

 

June 24, 2017

            Phil Cardoso (chair) called the meeting back to order at 8:00 AM.

            State reports continued

            Collaboration development

            10:30 AM Meeting adjourned

Accomplishments

CO Report- Immune responses related to BVDV infection and other virus challenge placental function, IFN expression and pregnancy outcomes. In vivo gene editing of CSH will allow for better understanding of its role in glucose transporters of the placenta.

MO Report- Estrus expression in relation to pregnancy rate in FTAI scenarios. Circulating concentrations of E2 during the preovulatory period does not affect conceptus elongation or pregnancy rates in beef heifers at day 16 of gestation.

AZ Report- Oxygen and glucose supplementation will reduce the endocrine stress response in fetuses with placental insufficiency-induced intrauterine growth restriction. DBP, a common environmental contaminant and its main substitute (ATBC), adversely affect the growth and survival of ovarian antral follicles. Enzyme involved in β-oxidation were upregulated in IUGR fetuses.

 

IL Report- Dairy cows supplemented with Methionine and/or Choline differed in gene expression than control cows but ovulation and P4/E2 concentrations were not affected. Studies were conducted to validate E2 and P4 commercial EIA assays with bovine serum.

 

KS Report- Estrus detection patches were used to evaluate delaying insemination in heifers that did not express estrus prior to TAI; however delaying insemination did not improve pregnancy rates in heifers that did not express estrus. Exercise may provide benefits to heat tolerance in dairy heifers without adverse effects on gestation or calving.

 

MS Report- Doppler ultrasound was used to evaluate uterine perfusion. Melatonin supplementation increases uterine blood flow but in the current study had no effect on calf weight at birth but there was differences postnatally. 

 

ND Report- Doppler ultrasound was used to monitor uterine dynamics and pregnancy in supplemented cows and ewes carrying twins vs singles. Binucleate cell and placental morphology were evaluated in heifers treated with bST and ewes fed different protein levels.

 

NE Report- VEGFA treatment in the high A4 herd was used to evaluate potential for follicular response and potential for follicle rescue. The A4 herd was found to have altered metabolism, increased folliculogenesis and change granulosa cell proliferation.

 

WA Report- Differences in gene expression in mice were evaluated to better understand oxygen availability to the fetus through the placenta. Mice were also used in a human reproductive cancer study.

 

AK Report- Synthetic progesterone supplementation on rutting reindeer calmed aggressive and did not negatively impact semen characteristics. The next study will increase numbers for a preliminary trial of methionine supplementation in muskoxen.  

TX Report- Brahman heifers that calve around two years of age have different growth patterns than heifers which calve later. Progeny of bulls who underwent prenatal stress have different responses to endotoxin challenges and altered glucose homeostasis. In addition many factors including sex, reproductive status and age influence immune responses in Brahman cattle.

CT Report- Maternal nutritional stress reduces offspring muscle growth through changes in Pax7 abundance and other gene expression involved in in cell signaling, inflammation, and epigenetic regulation.  Maternal nutrition affects the DNA methylation patterns in the pancreas and markers of inflammation in the liver.

PA Report- Work on the male fertility SNP chip continues. Studies into the role of PRAMEY during fertilization have been done in mutant mouse models and bovine in vitro models using abattoir derived spermatozoa. Creation of a KO line of PRAME mice is in development.

NM Report- Our results highlight the importance of the positive-feedback loop between VEGF and CXCL12-CXCR4 signaling and that CXCL12-CXCR4 signalling impacts inflammatory cytokine expression. In applied strategies research, delaying heifer growth does not improve reproductive parameters and a PG synchronization in ewes results in similar estrus expression results as a CIDR protocol.

UT Report- Focus on statistical methodologies in relation to gene expression, FDR and oocyte viability.

WY Report- Progress has been made towards understanding the station objectives in terms of maternal obesity’s effect on offspring and further generations, importance of male sexual behavior, impact of extended release FSH treatment in bulls and maternal/fetal cortisol on metabolic programming.

TN Report – Early gestation circulating PAG concentration may have application in diagnosing pregnancy at day 24 gestation and more work is needed to determine the potential of early gestation PAGs predicting embryo loss in dairy and beef.

 

Collaborations (2017):

Mississippi State and Montana State - Effects of maternal nutrient restriction in Angus and Brahman heifers on placental blood flow and fetal development related to the hypothalamus and liver.

 

Uconn and CSU - Use lentiviral approach to knockdown expression of chemokine receptors (CXCR4 or CXCR7) in trophoblast to further define the role of each in placental development. 

AK, NE, and WY - Tissue collection (testes [NE] and brain [WY]) to examine for difference between Depo Provera treated and control reindeer bulls during rut (breeding season).

AK and TN - Blood sample collection from pregnant reindeer to examine for PAG.

Mississippi state and Nebraska will continue collaborating on Liver enzymes in HIgh and Low A4 cows and pubertal classifications

 

California and Neb will collaborate on follicle isolation and culturing techniques as well as sharing Endocrinology teaching notes

 

Wyoming and Nebraska will collaborate on examining hypothalamus from different pubertal classifications which may become High A4 cows

 

Wyoming, Alaska and Nebraska will collaborate on examining testes and hypothalamus from reindeer, caribou and sheep.

 

ND, CO, and CT: maternal nutrition impacts on placental function, nutrient transport, and fetal metabolism and growth

 

ND, MS: maternal nutritional impacts on uterine/umbilical blood flow, mammary blood flow and endocrine profiles.

 

Kim Vornahme (NDSU): Evaluate the effects of nutrient restriction and realimentation on fetal liver and muscle growth and metabolism

 

Ryan Ashley (NMSU): Effect of maternal nutrient restriction and overfeeding during gestation on CXCR4 and CXCL12 placenta

 

John Stevens (Utah State Univ): Effect of maternal diet on offspring muscle gene expression

 

TN and TAMU: Effect of subspecies on PAG profiles during gestation.

Impacts

  1. successfully validated commercially available enzyme immunoassay (EIA) for progesterone (P4) and estradiol (E2) using extracted bovine serum. The E2 EIA may be an acceptable alternative to an radioimmunoassay (RIA) in samples with lower E2 concentrations. The P4 EIA can also be used as an alternative to RIA and the relationship found in this study can be used as a model to predict RIA results.
  2. Cold extraction method described in our study can be used to monitor procedural losses and will yield similar efficiencies to a method using tritiated hormones. In conclusion, if the goal of a researcher is to eliminate the use of a radioactive marker when quantifying E2 or P4 in bovine serum, the EIA and cold extraction methods are valid alternatives.
  3. Creating CSH-deficient pregnancies has revealed that this placental hormone impacts fetal growth early in gestation, and its deficiency also results in placental and fetal growth restriction near-term. The early effects on fetal growth may result from CSH impacting placental glucose transport
  4. Understanding how maternal viral infections impact the fetus during development may lead to strategies that can be used to better manage pregnancies at risk as well as postnatal health and well-being of offspring in ruminants and other animals.
  5. Preovulatory estradiol has a positive effect on the establishment and maintenance of pregnancy by an intrafollicular action on granulosa cells to enhance luteinization and (or) an extrafollicular effect on the uterus to promote conceptus elongation and placentation.
  6. Fetal stress negatively affects development, maturation, and metabolism during placental insufficiency
  7. LXRs and SREBF2 may regulate cholesterol supply to the corpus luteum and represent a novel mechanism regulating luteal function during the luteal phase and luteolysis.
  8. The use of estrus detection patches can assist producers in choosing heifers with the greatest chance of conceiving to AI in a fixed time estrous synchronization protocol.
  9. Exercise in pregnant dairy heifers may improve heat tolerance, and improve milk quality during first lactation without adverse effects on gestation or parturition.
  10. Maternal melatonin implants in the last trimester of gestation increase uterine artery blood flow, postnatal growth and scrotal circumference at weaning of offspring.
  11. Supplementation with corn can be used as a substitute when forage is limited as it does not negatively impact calf growth or uterine blood flow.
  12. A single i.m. injection of 400 mg medroxy progesterone acetate (Depo-Provera) was sufficient to reduce aggressive and rut related behavior, maintain food consumption and significantly reduce rut related weight loss, without blocking fertility in reindeer bulls. The treated bulls behaved as functional castrates.
  13. Increases in circulating concentrations of PAG early in gestation are predictive of the developing embryo’s success. Pregnancy/embryonic viability detection is possible with PAGs as early as day 24 of gestation; however, embryonic mortality between day 24-31 of gestation may be problematic for earlier pregnancy detection.
  14. Poor maternal nutrition, under- and/or over-feeding, beginning at d 30 of gestation alters expression of key pathways involved in muscle development and markers of stem cell number which may be mechanisms contributing to altered muscle growth and causes epigenetic modification to genes in offspring pancreas in a gender-dependent manner.
  15. the PRAME/PRAMEY gene family is essential for spermiogenesis and male fertility, which provide insights into the molecular mechanism of spermatogenesis
  16. Prame and Pramel1 KO mice provide an excellent model to study the functional role of the PRAME gene family
  17. Understanding CXCL12-CXCR4 signaling regulates angiogenic factors at the fetal-maternal interface may enhance our understanding of the mechanisms driving placental growth and vascularization
  18. Administering 10 mg Lutalyse 2.5 d after ram turn out resulted in a similar proportion of ewes being marked after 5 d of the breeding season when compared to a 12-d CIDR protocol
  19. The tolerance interval approach is a satisfactory statistical method to assess oocyte viability using gene expression profiles.
  20. When seeking to control the false discovery rate (FDR) in high-throughput testing for differential expression, in the presence of dependence among the tested biomarkers, it is best to use the two stage Benjamini and Hochberg procedure, the q-value method, or the adaptive Benjamini and Hochberg procedure, but not the principal factor approximation method.
  21. FSH-HA treatment positively affects the hypothalamo-pituitary-gonadal axis of bulls by stimulating the feedback loop involving activin A and FSH production.
  22. the optimization of maternal nutrition and the associated uterine environment will contribute to the birth of healthy, growth-efficient offspring, which is vital to livestock production efficiency.

Publications

  • S. Skenandore, A. Pineda, J.M. Bahr, A.E. Newell-Fugate, and F.C. Cardoso. (2017). Evaluation of a commercially available radioimmunoassay and enzyme immunoassay for the analysis of progesterone and estradiol and the comparison of two extraction efficiency methods. Domestic Animal Endocrinology. 60:61-66.
  • Acosta, D.A., M.I. Rivelli, C. Skenandore, Z. Zhou, D.H. Keisler, D. Luchini, M.N. Corrêa, and F.C. Cardoso. (2017). Effects of rumen-protected methionine and choline supplementation on steroidogenic potential of the first postpartum dominant follicle and expression of immune mediators in Holstein cows. Theriogenology. 96:1-9.
  • Rivelli, M.I., S.Y. Morrison, K.J. Haerr, S. Rodriguez-Zas, and F.C. Cardoso. (2017). Nutrition, reproduction, and young stock performance in dairy farms throughout Illinois: a Dairy Focus Team approach. The Professional Animal Scientist. In Press.
  • S. Skenandore, D.A. Velasco-Acosta, Z. Zhoua, M.I. Rivelli, M.N. Corrêa, D.N. Luchini, and F.C. Cardoso. (2017). Effects of rumen-protected methionine and choline supplementation on vaginal discharge and uterine cytology of Holstein cows. International Journal of Veterinary Science and Medicine. 5:1-7.
  • Kennedy, V. C., B. R. Mordhorst, J. J. Gaspers, M. L. Bauer, K. C. Swanson, C. O. Lemley, and K. A. Vonnahme. 2016. Supplementation of corn dried distiller’s grains plus solubles to gestating beef cows fed low-quality forage: II. Impacts on uterine blood flow, circulating estradiol-17β and progesterone and hepatic steroid metabolizing enzyme activity. Journal of Animal Science. 94:4619-4628.
  • Gilfeather, C. L. and C. O. Lemley. 2016. Effects of interferon-tau and steroids on cytochrome P450 activity in bovine endometrial epithelial cells. Reproduction in Domestic Animals. 51:415-420.
  • Prezotto, L. D., L. E. Camacho, C. O. Lemley, F. E. Keomanivong, J. S. Caton, K. A. Vonnahme, and K. C. Swanson. 2016. Nutrient restriction and realimentation in beef cows during early- and mid-gestation and maternal and fetal hepatic and small intestinal in vitro oxygen consumption. Animal. 10:829-837.
  • Brockus, K. E., C. G. Hart, B. O. Fleming, T. Smith, S. H. Ward, and C. O. Lemley. 2016. Effects of supplementing Holstein heifers with dietary melatonin during late gestation on growth and cardiovascular measurements of their offspring. Reproduction in Domestic Animals. 51:240-247.
  • Keomanivong, F. E., C. O. Lemley, L. E. Camacho, R. Yunusova, P. P. Borowicz, J. S. Caton, A. M. Meyer, K. A. Vonnahme, and K. C. Swanson. 2016. Influence of nutrient restriction and melatonin supplementation of pregnant ewes on maternal and fetal pancreatic digestive enzymes and insulin-containing clusters. Animal. 10:440-448.
  • Cline, G. F., A. M. Muth-Spurlock, B. E. Voelz, C. O. Lemley, and J. E. Larson. 2016. Evaluating blood perfusion of the corpus luteum in beef cows during fescue toxicosis. Journal of Animal Science. 94:90-95.
  • Brockus, K. E., C. G. Hart, C. L. Gilfeather, B. O. Fleming, and C. O. Lemley. 2016. Dietary melatonin alters uterine artery hemodynamics in pregnant Holstein heifers. Domestic Animal Endocrinology. 55:1-10.
  • Mordhorst, B.R., C.A. Zimprich, L.E. Camacho, M.L. Bauer, and K.A. Vonnahme. 2016. Supplementation during late gestation in beef cows consuming low quality forage decreases uterine blood flow without impacting mammary gland blood flow. Journal of Animal Physiology and Animal Nutrition. doi: 10.1111/jpn.12580.
  • Reynolds, L.P and K.A. Vonnahme. 2016. Triennial Reproduction Symposium. Developmental programming of fertility. Journal of Animal Science 94:2699-2704.
  • Kennedy, V.C., M.L. Bauer, K.C. Swanson, and K.A. Vonnahme. 2016. Supplementation of corn dried distiller’s grain plus solubles to gestating beef cows fed low-quality forage: I. Altered intake behavior, body condition, and reproduction. Journal of Animal Science. 94:240-247.
  • Keomanivong, F. E., L.E. Camacho, C.O. Lemley, E.A. Kuemper, R. Yunusova, P.P. Borowicz, J.D. Kirsch, A. Vonnahme, J.S. Caton, and K.C. Swanson. 2016. Effects of realimentation after nutrient restriction during mid- to late-gestation on pancreatic digestive enzymes, serum insulin and glucose levels, and insulin-containing cell cluster morphology. Journal of Animal Physiology and Animal Nutrition. doi: 10.1111/jpn.12480.
  • Prezotto, L.D., L. E. Camacho, C. O. Lemley, F. E. Doscher, J. S. Caton, K. A. Vonnahme, and K. C. Swanson. 2016. Nutrient restriction and realimentation in beef cows during early- and mid-gestation and maternal and fetal hepatic and small intestinal in vitro oxygen consumption. Animal. 10:829-837. doi:10.1017/S1751731115002645.
  • Khanthusaeng V, Thammasiri J, Bass CS, Navanukraw C, Redmer DA and Grazul-Bilska AT. Lipid droplets in cultured luteal cells in non-pregnant sheep fed different planes of nutrition. Acta Histochemica, 118:553-559, 2016.
  • Quinn KE, LP Reynolds, AT Grazul-Bilska, PP Borowicz, RL Ashley. Placental development during early pregnancy: effects of embryo origin on chemokine ligand twelve (CXCL12). Placenta 43: 77-80, 2016.
  • Bairagi S, KE Quinn, AR Crane, RL Ashley, PP Borowicz, JS Caton, RR Redden, AT Grazul-Bilska, LP Reynolds. Maternal Environment and Placental Vascularization in Small Ruminants. Theriogenology. 86:288-305, 2016.
  • Quinn, K.E., S.Z Prosser, K.K. Kane, and R.L. Ashley. 2017. Inhibition of chemokine (C-X-C motif) receptor four (CXCR4) at the fetal-maternal interface during early gestation in sheep: alterations in expression of chemokines, angiogenic factors and their receptors. J. Anim. Sci. 95(3):1144-1153. PMID: 28380526
  • Salazar, M.S., A. Lerma-Ortiz, G. M. Hooks, A. Alire, A. K. Ashley and R. L. Ashley. 2016. Progestin-mediated activation of MAPK and AKT in nuclear progesterone receptor negative breast epithelial cells: the role of membrane progesterone receptors. Gene. 591(1):6-13. PMCID: PMC4992424
  • Quinn, K.E., L.P. Reynolds, A.T. Grazul-Bilska, P.P. Borowicz, and R.L. Ashley. 2016. Placental development during early pregnancy: Effects of embryo origin on expression of chemokine ligand twelve (CXCL12). Placenta. 43. 77-80. PMID: 27324103
  • Hill, S.L., M. Grieger, K.C. Olson, J.R. Jaeger, C.T. Dahlen, M.R. Crosswhite, N.N. Pereira, S.R. Underdahl, B.W. Neville, J.K. Aholoa, M.C. Fisher, G.E. Seidel, and J.S. Stevenson. Gonadotropin-releasing hormone increased pregnancy risk in suckled beef cows not detected in estrus and subjected to a split-time artificial insemination program. J. Anim. Sci. 2016 Sep;94(9):3722-3728. doi: 10.2527/jas.2016-0582.
  • White, L., J. M. Bormann, K. C. Olson, J. R. Jaeger, S. Johnson, B. Downey, D.M. Grieger, J. W. Waggoner, D. W. Moser and R. L. Weaber. Phenotypic relationships between docility and reproduction in Angus heifers.  J. Anim. Sci. 2016 Feb;94(2):483-9. doi: 10.2527/jas.2015-9327.
  • Stevens J.R., Al Masud A., and Suyundikov A. (2017) “A comparison of multiple testing adjustment methods with block-correlation positively-dependent tests.” PLOS ONE 12(4): e0176124. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0176124
  • Brown, L.D., M.A. Davis, S. Wai, S.R. Wesolowski, W.W. Hay Jr., S.W. Limesand, R.J. Rozance (2016). Chronically increased amino acids improve insulin secretion, pancreas vascularity, and islet size in growth restricted fetal sheep. Endocrinology 157(10):3788-3799.
  • Chen, X., A.C. Kelly, D.T. Yates, A.R. Macko, R.M. Lynch, and S.W. Limesand (2017) Islet adaptations in fetal sheep persist following chronic exposure to high norepinephrine. Journal of Endocrinology 232(2):285-295. Epub 2016 Nov 25.
  • Harris, S.E., M. De Blasio, M.A. Davis, A.C. Kelly, H.M. Davenport, P. Wooding, D. Blache, D. Meredith, M.J. Anderson, A.L. Fowden, S.W. Limesand, and A.J. Forhead (2017). Hypothyroidism in utero stimulates pancreatic beta cell proliferation and hyperinsulinaemia in the ovine fetus during late gestation. Journal of Physiology 595(1):3331-3343.
  • Benjamin, J., C. Culpepper, L.D. Brown, S.R. Wesolowski, S. Jonker, M.A. Davis, S.W. Limesand, R.B. Wilkening, W.W. Hay, Jr., and P.J. Rozance (2017). Chronic Anemic Hypoxemia Attenuates Glucose-Stimulated Insulin Secretion in Fetal Sheep. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 312(4):R492-R500.
  • Kelly, A.C., C.A. Bidwell, F.M. McCarthy, D.J. Taska, M.J. Anderson, L.E. Camacho, and S.W. Limesand (2017). RNA sequencing exposes novel adaptive and immune responses to intrauterine growth restriction in fetal sheep islets. Endocrinology. 158(4):743-755.
  • Limesand SW and PJ Rozance (2017). Fetal adaptations in insulin secretion result from high catecholamines during placental insufficiency. Journal of Physiology (in press) Feb 14. doi: 10.1113/JP273324. [Epub ahead of print]
  • Smith, KE, AC Kelly, CG Min, C Weber, FM McCarthy, LV Steyn, V Badarinarayana, P Strop, RM Lynch, SW Limesand, KK Papas (2017). Acute ischemia induced by high density culture increases cytokine expression and diminishes the function and viability of highly purified human islets of Langerhans. Transplantation (in press).
  • Camacho, LE, X Chen, WW Hay Jr, SW Limesand (2017). Enhanced insulin secretion and insulin sensitivity in young lambs with placental insufficiency-induced intrauterine growth restriction. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology (in press).
  • Jensen, J.T., I.B. Addis, J.D. Hennebold, R.L. Bogan (2017). Ovarian lipid metabolism modulates circulating lipids in premenopausal women. J Clin Endocrinol Metab [Epub ahead of print January 6, 2017], DOI:10.1210/jc.2016-3456.
  • Rasmussen LM, Sen N, Liu X, Craig ZR. Effects of oral exposure to the phthalate substitute acetyl tributyl citrate on female reproduction in mice.  J Appl Toxicol 2017; 37:668-675.
  • Rasmussen LM, Sen N, Vera JC, Liu X, Craig ZR. Effects of in vitro exposure to dibutyl phthalate, mono-butyl phthalate, and acetyl tributyl citrate on ovarian antral follicle growth and viability.  Biol Reprod 2017; 96:1105-1107.
  • Gates, K.C. L.N. Goetzmann, J.D. Cantlon, K.M. Jeckel and R.V. Anthony. 2017. Effect of proline rich 15-deficiency on trophoblast viability and survival. PLoS One 2:e0174976
  • Odle, J. S.K. Jacobi, R.D. Boyd, D.E. Bauman, R.V. Anthony, F.W. Bazer, A.L. Lock, and A.C. Serazin. 2017. The Potential Impact of Animal Science Research on Global Maternal and Child Nutrition and Health: A Landscape Review. Adv. Nutri. 8:362–381.
  • Clark NC, Pru CA, Yee SP, Lydon JP, Peluso JJ, Pru JK. Conditional ablation of progesterone receptor membrane component 2 causes female premature reproductive senescence. Endocrinology 2017;158:640-651. PMID:28005395
  • Kelleher AM, Peng W, Pru JK, Pru CA, DeMayo FJ, Spencer TE. Forkead box a2 (FOXA2) is essential for uterine function and fertility. Proc Natl Acad Sci 2017;114:E1018-E1026. PMID:28049832
  • Clark NC, Pru CA, Pru JK. Novel regulators of hemodynamics in the pregnant Pro Mol Biol Transl Sci 2017;145:181-216. PMID:28110751
  • McCallum ML, Pru CA, Niikura Y, Yee SP, Lydon JP, Peluso JJ, Pru JK. Conditional ablation of progesterone receptor membrane component 1 results in subfertility in the female and development of endometrial cysts. Endocrinology 2016;157:3309-3319 PMID:27309940
  • Romereim, S, AS Cupp. Book Chapter- Invited:  Mesonephric Cell Migration Into the Gonads is a Process Crucial for Testis Development" in R.P. Piprek (ed.), Molecular Mechanisms of Cell Differentiation in Gonad Development, Results and Problems in Cell Differentiation 58, 2016 DOI 10.1007/978-3-319-31973-5_4.
  • Romereim SM, AF. Summers, WE. Pohlmeier, P Zhang, X Hou, HA Talbott, RA. Cushman, JR. Wood, JS Davis, AS. Cupp. Gene Expression Profiling of Bovine Ovarian Follicular and Luteal Cells Provides Insight into Cellular Identities and Functions. Epub ahead of print 2016 Dec 10; Molecular and Cellular Endocrinology, Volume 439, 5 January 2017, Pages 379-394. DOI: 10.1016/j.dib.2016.11.093.
  • Romereim SM, Summers, WE. Pohlmeier, P Zhang, X Hou, HA Talbott, RA. Cushman, JR. Wood, JS Davis, AS. Cupp. Data In Brief: Transcriptomes of bovine ovarian follicular and luteal cells. Mol Cell Endocrinol. Epub ahead of Print Dec 15, 2016; http://dx.doi.org/10.1016/j.dib.2016.11.093.
  • McNeel, AK, ÉM. Soares, AL Patterson, JL Vallet, EC Wright, EL Larimore, OL  Amundson, JR  Miles, CC  Chase Jr., CA Lents, JR Wood, AS Cupp, GA Perry, Cushman.  Beef heifers with diminished numbers of antral follicles have decreased uterine protein secretion. Anim Reprod Sci. 2017 Jan 12. pii: S0378-4320(16)30618-2. doi: 10.1016/j.anireprosci.2017.01.004.
  • Talbott H, Hou X, Qiu F, Zhang P, Guda C, Yu F, Cushman RA, Wood JR, Wang C, Cupp AS, Davis JS. Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling. Mol Cell Endocrinol. 2017 May 23. pii: S0303-7207(17)30287-3. doi: 10.1016/j.mce.2017.05.018. [Epub ahead of print].
  • Govoni, K., Reed, S. A., (2017). How Mom’s diet affects offspring growth and health through modified stem cell function. Animal Frontiers. In Press.
  • Pillai, S. M., Jones, A. K., Hoffman, M. L., McFadden, K. K., Reed, S. A., Zinn, S., Govoni, K. (2017). Fetal and organ development at gestational days 45, 90, 135 and at birth of lambs exposed to under- or over-nutrition during gestation. Translational Animal Science, 1, 16-25.
  • Hoffman, M. L., Reed, S. A., Pillai, S. M., Jones, A. K., McFadden, K. K., Zinn, S., Govoni, K. (2017). The effects of poor maternal nutrition during gestation on offspring postnatal growth and metabolism. Journal of Animal Science, 94, 3093-3099.
  • Jones, A. K., Gately, R. E., McFadden, K. K., Hoffman, M. L., Pillai, S. M., Zinn, S., Govoni, K., Reed, S. A. (2017). Ultrasound during mid-gestation: Agreement with physical foetal and placental measurements and use in predicting gestational age in sheep. Reproduction in domestic animals Mar 23. doi: 10.1111/rda.12961.
  • Pillai, S. M., Sereda, N. H., Hoffman, M. L., Valley, E. V., Crenshaw, T. D., Park, Y.-K., Lee, J.-Y., Zinn, S., Govoni, K. (2016). Effects of Poor Maternal Nutrition during Gestation on Bone Development and Mesenchymal Stem Cell Activity in Offspring. PloS one, 11(12), e0168382.
  • Hoffman, M. L., Peck, K. N., Wegrzyn, J., Reed, S. A., Zinn, S., Govoni, K. (2016). Poor maternal nutrition during gestation alters the expression of genes involved in muscle development and metabolism in lambs. Journal of animal science, 94(7), 3093-9.
  • Liu, W.-S., Zhao, Y.Q., Lu, C., Ning, G., Ma, Y., Diaz, F., O'Connor, M. (2017) A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle. Reproduction 153, 847–863.
  • Maalouf, S.W., Liu, W.-S., Pate, J.L. (2016) MicroRNA in ovarian function. Cell Tissue Res 363, 7–18.
  • Demirkhanyan, L. V. Krishnan, S. Asuthkar, B. Alexander, Z. Hussain, P. Baskaran, Y. Nersesyan, E. Pavlov, B.Thyagarajan, and Zakharian. 2017. TRPM8 regulates mammalian dimorphic sexual behaviors. Nature Comm. In review.
  • Ferraz Junior, M.V., Pires, A.V., Biehl, M.V., Santos, M.H., Barroso, J.P., Goncalves, J.R., Sartori, R., Day, M.L., 2016a. Comparison of two timed artificial insemination system schemes to synchronize estrus and ovulation in Nellore cattle. Theriogenology 86, 1939-1943.
  • Ferraz Junior, M.V., Pires, A.V., Biehl, M.V., Santos, M.H., Polizel, D.M., Nepomuceno, D.D., Sartori, R., Barreto Filho, J.B., Goncalves, J.R., Day, M.L., 2016b. Luteolysis in Bos indicus cows on Days 5 and 7 of estrous cycle with varying doses of PGF2alpha. Theriogenology 86, 1268-1274.
  • Ghnenis, A. B., J. F. Odhiambo, J. McCormick, P. W. Nathanielsz, and S. P. Ford. 2017. Maternal Obesity in the ewe increases cardiac ventricular expression of glucocorticoid receptors, pro-inflammatory cytokines and fibrosis in Adult Male Offspring. Plos ONE. In review.
  • Harstine, B.R., Cruppe, L.H., Abreu, F.M., Utt, M.D., Cipriano, R.S., Lemes, A., Premanandan, C., DeJarnette, J.M., Day, M.L., 2017. Impact of a timed-release follicle-stimulating hormone treatment from one to three months of age on endocrine and testicular development of prepubertal bulls. Journal of Animal Science 95, 1669-1679.
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