Brief Summary of Minutes of Annual Meeting

I Approval of minutes: Minutes of the January 2003 meeting were distributed. Deb Hammernik moved the approval of minutes. Motion was seconded by Max Rothschild and unanimously approved.

II. Old Business
Cattle: Jim Womack presented the annual report of the species activities. The highlights included active support by the coordinator, NRSP8 participants and the cattle community developed a white paper and generated financial support for sequencing the cattle genome. The sequencing program was officially initiated in December 2003, and the first set of sequence data is expected to be made available by February/March 2004. The coordinator continues to maintain the RH-analysis website but has transferred other bovine genome database responsibilities to Database coordinator Jim Reecy at Iowa State University. DNA from the 5,000 and 12,000 rad panels is being distributed by the coordinator to all researchers interested in developing fine physical maps. Additionally, bovine BAC libraries, DNA from somatic cell hybrid panel, and DNA from the International Bovine Reference family panel (IBRP) are also being made available to scientists needing these mapping resources.
Cattle administrative advisor Colin Kaltenbach lauded the efforts and industry representative Elizabeth Dressler promised continuous support and interest of the industry in enhancing research strengths of the group.

Sheep: Noelle Cockett reported the progress currently being made through the coordinator?s funds to develop, analyze and distribute the ovine radiation hybrid panel. Efforts are underway to develop a framework map for the sheep genome with ~2,500 loci. A memorandum of understanding has been signed with Peter de Jong (BACPAC resources) to acquire the sheep BAC library, make copies and develop high density filters for distribution to the sheep gene mapping community. Progress on the BAC end sequencing and fingerprinting was reported. Currently end sequencing of 150,000 to 200,000 BACs is being carried out at INRA (France) and Roslin Institute (UK) in conjunction with NRSP8 sheep coordinator. A seed grant from Utah State University is currently being used to end sequence some (unspecified number) ovine BACs. Sheep administrative advisor Colin Kaltenbach commended the work of the sheep group and described them as strong and highly regarded internationally.

Equine: Ernie Bailey provided an overview of the equine workshop. The invited presentations focused on bioinformatics and MHC. He elaborated the coordinated progress of the equine group in the areas of RH and linkage mapping and defined the goals agreed upon by the community for the next year. Publication of the first generation RH map in horse was a highlight. Progress made at the Havermeyer International Equine Genomics meeting in South Africa was also briefly mentioned. Coordinator funds were used for development and distribution of resources (reference family material, genome scan panel, etc.), and for travel support to technical committee members and a graduate student for PAGXII. Horse administrative advisor Bill Trumble pointed out that despite a late start, the horse group has done an amazing job.

Poultry: Jerry Dodgson reported that the PAG XII chicken workshop was the best the group ever had. The first generation physical map was ready in December; the 2nd generation map will probably be obsolete with the sequence information being made available soon. The current focus of the chicken community is primarily on the sequencing project ? mainly getting the finished sequence data. The linkage map generated up till now was reported to be well used. He emphasized the importance of NRSP8 in getting the sequencing efforts on track, making the East Lansing International reference family DNA, primer pair kits, BAC libraries filters and PCR-screenable pooled DNA (superpools) and other resources to several laboratories around the world. He also mentioned the availability of chicken microarray from FHCRC (Jeff Delrow and Paul Neiman). The Poultry Genome News letter supported through NRSP8 is constantly providing updates and details on research activities to researchers around the world. On behalf of the poultry administrative advisor, Margaret Dentine greatly appreciated the efforts of the poultry gene mapping group.

Pig: Max Rothschild reported that the swine genome workshop had the biggest crowd compared to earlier meetings (~120 people). Several developments were reported including: active use of markers by the swine industry, constant rapid expansion of the maps, availability of 2 RH panels (one from MN/INRA and the other from Reno, Nevada), etc. Intense efforts being made by Larry Schook and the entire pig community to get funding for sequencing the pig genome was a major activity of the group. Submission of the white paper was mentioned. Efforts made by the entire pig community to generate funds to the tune of $20 million were stated. This includes requests made to the Pork Board. Further, the importance of NRSP8 in facilitating database activities, distribution of various resources/materials including the Pig Genome Update, and providing travel and meeting support were acknowledged. Swine administrative advisor Margaret Dentine was very supportive of the intense efforts made by the swine genomics group in development of a high resolution map and sequencing.

With permission from the Chair, Larry Schook requested to discuss about the critical situation pig researchers are facing for generating matching funds required for getting the pig genome sequencing on track. On behalf of the Swine sequencing committee, Schook requested for ?support and endorsement from the NRSP8 group to empower USDA/NRI to provide financial support because it is in the best interest of the entire animal genome community?. The motion was moved for approval.

Jim Womack (TAMU) supported the motion stating that the window of opportunity is narrow and all efforts must be made to capitalize on what is available. He requested the group to embrace the pig genome sequencing efforts.

Noelle Cockett (U of Utah) wanted to know the current status regarding the availability of funds for sequencing (primarily the amount that has been committed) and how much would be needed.

Larry Schook replied that $20M needs to be generated by October 1. He mentioned about some international commitments and various steps (not specified) that are being taken to achieve the target.

Max Rothschild clarified that the pig community has at least $10M and is reasonably comfortable to raise another $5M for whole genome sequencing. It is the remaining $5M that needs to be obtained from some source.

Chris Biddwell wanted to know whether support of the motion automatically implies availability of lesser federal (USDA/NRI) funds for competitive proposals.

It was finally suggested that the Chair of the meeting (F. Abel Ponce de León) will draft a letter of support and circulate it to chairs of different species committees, get a consensus and then send it to USDA/NRI-CREES.

Finally, Max Rothschild (ISU) suggested adoption of two motions:
Motion 1: NRSP8 group is supportive of efforts for sequencing the swine genome
Motion 2: The group is also supportive of the efforts by the USDA to find additional financial resources to support the swine sequencing effort.

Questions were raised whether this will authorize USDA to provide financial support to the pig group (Noelle Cockett, U of Utah). There were propositions that other agencies like DOE must be approached. Schook said that the possibility to get funds from DOE is bleak.

At the end of the discussion, the motion: ?The NRSP8 group is supportive of the efforts for sequencing the swine genome, and the group recommends USDA to make efforts to find financial resources supporting the sequencing project?. Deb Hamernik clarified that this does not bind USDA in anyway to commit financial resources to the pig genome sequencing project.

The motion was carried unanimously with Max Rothschild moving it and Jim Womack seconding it.

Database: James Reecy summarized about the presentations he made to different species workshops. He reiterated his eagerness to hear from species coordinators and participants regarding the database needs of individual groups.

Aquaculture: John Liu reported that the rather newly formed aquaculture group is very diverse with 6 different species. This year?s workshop organized by Tom Kocher was very strong in science. International participation was strong, especially by Tilapia researchers. Prospects of sequencing salmon in the near future were discussed.

Administrators reports:

Lead Administrative advisor Margaret Dentine reported about the new five year cycle for NRSP8 program. She welcomed the aquaculture group within the fold of the program. The participants were informed about the setting up of a database that is expected to provide detailed information about the project. All participants (~72, with 6 new from Beltsville) were asked to fulfill their reporting commitments on time because documentation of progress is important for the project. The new members were requested to provide complete addresses and e-mails, and current members were requested to update their e-mail addresses (if changed). Margaret said that the NRSP8 project is indeed in good shape and the progress made is excellent.

Program Leader Muquarrab Qureshi introduced himself to the NRSP8 community and provided an overview of CSREES activities. A summary of National Public Funding and the latest NRI funding was given. Stating that these are exciting times for genomics, he said that he will take the message regarding various developments to Washington DC, in particular the developments relating to sequencing efforts currently underway in chicken and cattle. He provided a brief overview of the ?Discover? conference, thanked species coordinators for providing impact statements for the new CSREES website, and mentioned about the current stand of USDA regarding consumption of cloned and genetically engineered food. Muquarrab expressed great enthusiasm to be a part of the NRSP8 community. He looked forward to regularly receiving constructive comments and suggestions for improvement in the program.

III New business
1. Meeting day and time for 2005: It was agreed that in January 2005, the executive committee of NRSP8 will meet at noon (instead of early morning; as has been the case in the past). Further, it was also unanimously agreed that in January 2005, the NRSP8 annual meeting will be held on Sunday, one day before the start of the PAG meeting, from 4:30 to 6:00 PM (instead of late evening or night). These changes will be reviewed after 2005 PAG meeting.

2. Election of officers for 2004: Tom Kocher (New Hampshire) was nominated and unanimously elected Secretary of the NRSP8 Animal Genome Technical Committee for 2004-2005. Current Chair Abel Ponce de León thanked everyone present for participation and fruitful discussions and handed over the responsibilities for the next year to current secretary Bhanu Chowdhary.

3. Selection of next meeting location and date: The next NRSP8 meeting will be held in conjunction with PAGXIV.

IV Adjournment: The meeting was adjourned at 8:45 P.M. with a vote of thanks to F. Abel Ponce de León for a very successful organization of the meeting.

Progress Toward Objective 1:

Aquaculture:
Catfish ? AL has constructed a genetic linkage map for catfish containing 418 AFLP markers. This work has been published (see below under publications). EST project has now 4,103 gene-associated microsatellites and 145 gene-associated SNP markers.
Rainbow trout - NCCCWA have developed more microsatellite and single nucleotide polymorphism markers for mapping in salmonid and Morone species. A high-density genetic map for rainbow trout is expected to be continued through 2004.
Tilapia - NHAES has completed a genetic linkage map for tilapia containing more than 550 microsatellite and gene-based markers. A physical map based on the restriction fingerprints of 35,000 BAC clones (5x genome coverage) has also been completed (http//:www at hcgs.unh.edu)
Oysters (Crassostrea virginica and C. gigas) - NJ has generated AFLP-based linkage maps for the eastern and Pacific oysters. VIMS and DE have analyzed inheritance of a set of microsatellite markers in the eastern oyster observing a high incidence of null alleles and distorted segregation ratios.
Clams ? VA have initiated work on genetic improvement of the hard clam Mercenaria mercenaria. To date, four microsatellite libraries have been developed. Efforts have begun at VIMS to develop a set of Type I SNP markers for the hard clam. Of the 8 loci screened to date in a panel of clams from diverse commercial stocks, six have shown polymorphisms, with several containing more than two alleles.


Cattle:
Collaboration between TX and IL has produced a second-generation 5000 rad radiation hybrid (RH) EST map of the cattle genome covering gaps in the existing cattle-human comparative map as well as sparsely populated map intervals. A cattle-human comparative map containing 1463 comparative anchor points has been created using human genome sequence coordinates for paired orthologs to define boundaries of conserved chromosome segments. Approximately 312,000 BAC end sequences are in the public domain as a result of this collaborative effort, and a minimal tiling path of BACs for the bovine genome-sequencing project has been generated by an international consortium (including TX, IL, USDA-ARS). The class IIB and class III regions of the bovine MHC have been sequenced (TX). Future plans include development of a 1MB resolution comparative map (TX, IL), a bovine HapMap (TX) and finished sequence for the class IIB and class III regions of the bovine MHC (TX). The bovine genome is currently being sequenced making sequence annotation and integration with map data a high priority.

Horse:
The latest reiteration (MN, CA, Japan Laboratory of Racing Chemistry) and England Animal Health Trust, Newmarket) of the horse linkage map includes 801 markers and covers all autosomes with an average spacing of 6.8 cM. A radiation hybrid map (TX) now includes over 2000 markers. BAC libraries are being characterized and new markers being developed at TX, MN, France and Germany. A BAC contig has been developed (TX, NY) as well. Efforts are underway to utilize conserved, heterologous or horse-specific primers to generate 1-5 Mb marker density on selected equine chromosomes.

Poultry:
Numerous labs have cooperated in mapping DNA-based polymorphic markers by genotyping samples on three international reference crosses, the Compton population, the East Lansing population and the Wageningen population. The consensus map includes 1965 markers, placed into 50 linkage groups, covering around 4000 cM. Several laboratories have cooperated to generate a second generation BAC contig map comprised of about 280 contigs, two-thirds of which have been anchored to the genetic linkage/chromosome map. The Washington U. Genome Sequencing Center (WUGSC) has completed ~6X sequencing of the chicken genome (primarily whole genome shotgun) and this is now being assembled.

Sheep:
An ovine whole-genome radiation hybrid (RH) panel of 5,000 rad has been constructed (TX, UT). The resulting ovine framework/comparative map will contain about 500 microsatellite markers previously assigned to ovine and bovine linkage maps and about 500 ovine ESTs with known human map locations. A 10-fold redundant BAC library has been purchased from BACPAC Resources (AgResearch (New Zealand), the National Meat and Livestock Board (Australia), the USDA/ARS Meat Animal Research Center (Nebraska), and the NRSP-8 Sheep Coordinator, UT).

Swine:
Physical and genetic maps of the pig genome continue to be enhanced (BARC, IA, MI, NC, WA, MN, NE, and NV) through mapping of candidate genes, EST, SNP, and microsatellite markers using RH mapping panels, FISH, and reference population resources. Significant milestones toward the completion of Objective 1 include detailed comparative maps focusing on swine chromosomes 3 and 4, description of a second generation map of the IMpRH7000Rad RH mapping panel with an estimated 98% coverage of the human genome, and identification of polymorphisms within several new genes that may be important QTL.

Progress Toward Objective 2:

Aquaculture:
Catfish ? AL has continued to produce expressed sequence tags in channel catfish and blue catfish. Over 37,000 ESTs representing 25,334 unique sequences have been generated. These ESTs have been all deposited to GenBank. Using the EST resource, researchers have characterized expression of chemokine CXCL10 and CXCL8 in catfish with different resistance, both before and after infection.
Rainbow trout - NCCCWA has continued to identify expressed sequence tags in rainbow trout for identification of functional candidate genes affecting aquaculture production traits and the development of microarrays for functional genomics.
Tilapia - Sex linked markers have been identified on two linkage groups in Nile tilapia and blue tilapia. The locus on LG1 is an XY (male heterogametic) system. The locus on LG3 is a WZ (female heterogametic) system. Both loci segregated in a single family of blue tilapia. Work is underway to positionally clone these sex-determining genes.
Oyster ?SC led a successful effort to develop BAC libraries for both the eastern and Pacific oyster, receiving funding from NIH (National Human Genome Research Institute). NJ obtained 500+ sequences from suppression subtractive hybridization (SSH) libraries built to identify up-regulated genes in eastern oysters infected by Dermo and MSX (two parasitic pathogens). Analysis of these sequences has led to the identification of about 170 unique genes.

Cattle:
A first generation bovine adipose tissue microarray (OSU) comprised of 1100 unique cDNAs has been generated. A second bovine microarray has been constructed (IA) that includes 10,604 unique bovine cDNAs from the BOV1-4 cDNA libraries.
QTL mapping for various traits is underway (TX, WI, IL, CA). A new ovulation rate QTL was found (WI) on chromosome 14. Two regions show evidence of QTL segregation for ovulation rate or twinning rate in multiple families, and strong support for a previously reported BTA5 QTL \was found in a combined analysis of four related families. An F2 resource population (Bos taurus x Bos indicus) to investigate maternal reproductive traits is being developed (TX). Males will be used for feed efficiency experiments. A number of candidate genes for growth, disease resistance and reproduction have been evaluated in association studies (TX, NM). Mapping and identification of QTL provide the precursors for a better understanding of biological traits.

Horse:
About 11,000 high-quality, well characterized ESTs from stimulated and non-stimulated leukocytes were developed (GA) http://fungen.org/. ESTs (ca 14,000) from articular cartilage are being developed (KY) for the purpose of generating a microarray to study transcriptional changes in equine chondrocytes in osteoarthritis and other arthropathies. Additional ESTs are being developed at (MA, TX, NY). The use of genome mapping, comparative biochemistry and pathology and candidate gene evaluation resulted in the identification (MN) of a genetic change associated with glycogen storage disease IV (GBE1), a fatal disease in Quarter Horses.

Sheep:
The high growth (hg) mouse mutation is a 460 Kb deletion of mouse chromosome 10 which causes a 30-50% increase in growth in the homozygous animal. The research group (CA) has observed that the effect of hg can be modified dependent on the genetic background. They propose that the hg phenotype is influenced by genetic interactions between members of the Gh signaling pathway as a result of the absence of the SOCS-2 protein. A core cluster of imprinted genes (DLK1, GTL2, PEG11, and MEG8) located at the distal end of ovine chromosome 18A have been identified (UT, IN, USDA/ARS, and University of Liége). The callipyge (CLPG) mutation enhances the expression of genes in this core cluster when it is inherited in cis.
Interval mapping (LA) in an F2 population [Gulf Coast Native (resistant) and Suffolk (susceptible)] generated to identify chromosomal regions in the ovine genome that play a role in resistance to gastrointestinal parasites revealed a putative QTL localized on the central region of chromosome 1.

Swine:
IA, IL, MI reported progress toward the identification of QTL influencing the genetic regulation of meat quality traits. Also, BARC, IA, IN, MI, NC are using differential gene expression to identify genes controlling physiological traits of economic importance. Based on a microarray experiment NE identified approximately 100 genes putatively differentially expressed between reproductive selection lines. A number of QTL influencing meat quality traits have been identified, and fine mapping of causative genes is currently underway. Results from initial gene expression studies have identified gene expression patterns associated with muscle growth and development, immune responses, and tissue specificity. Additionally, the porcine long oligo microarray is being validated and will rapidly be put to use investigating a variety of experimental models.

Progress Toward Objective 3:

Aquaculture:
Catfish - A web-based EST database for catfish is under construction. Data mining of EST databases has generated large numbers of type I molecular markers.
Tilapia ? NHAES has developed WWW-based software for comparing the tilapia map with the linkage maps of medaka and zebrafish, and with the sequence scaffolds of pufferfish. The tilapia genetic and physical linkage maps are available in these browsers at hcgs.unh.edu

Cattle:
Two groups have begun to implement QTL databases on the web for bovine QTL data (CA,TX). One strategy has been to use the Generic Genome Browser (http://www.gmod.org) as the front end for a MySQL database. The other strategy has been to use the RatMap QTL browser (http://ratmap.org/qtler/) as the basis for an improved interface. Both databases will contain public domain QTL data and one of them will feature password protected log on for investigators to edit or submit QTL data prior to publication.

Horse:
The website at the University of Kentucky (http://www.uky.edu/Ag/Horsemap/) continues to be maintained and provides information describing workshop efforts, significant developments for the horse gene map. This site also provides links to other databases and communal resources. A graphic map viewer (HorseMap Viewer) is in final stages of development (CA) and will be also available to investigators.

Swine:
MN, NV, WA provided updates on their ongoing efforts to establish integrated database systems for multiple types of data relating to pig genes.

Aquaculture:

Argue, B., Liu, Z.J., and Dunham, R.A. 2003. Dress-out and fillet yields of channel catfish, Ictalurus punctatus, blue catfish, I. furcatus, and their F1, F2 and backcross hybrids. Aquaculture 228, 81-90.

Cnaani A, Lee B-Y, Ron M, Hulata G, Kocher TD, and Seroussi E. 2003. Linkage mapping of major histocompatibilty complex class I loci in tilapia (Oreochromis spp.). Animal Genetics 34:390-391.

He, C., Chen, L., Simmons, M., Li, P., Kim, S., and Liu, Z.J. 2003. Putative SNP discovery in interspecific hybrids of catfish by comparative EST analysis. Animal Genetics 34, 445-448.

Jenny, M. J., A. H. Ringwood, et al. (2002). "Potential indicators of stress response identified by expressed sequence tag analysis of hemocytes and embryos from the American oyster, Crassostrea virginica." Marine Biotechnology 4: 81-93.

Lee B-Y, Penman, DJ and Kocher TD. 2003. Identification of a sex-determining region in Nile tilapia (Oreochromis niloticus) using bulked segregant analysis. Animal Genetics 34(5): 379-383.

Li, L. and X. Guo. 2003. AFLP-based genetic linkage maps of the Pacific oyster Crassostrea gigas Thunberg. Marine Biotechnology, in press.

Liu, Z.J. 2003. A review of catfish genomics: progress and perspectives. Comparative and Functional Genomics 4, 259-265.

Liu, Z.J., Karsi, A., Li, P., Cao, D., and R. Dunham. 2003. An AFLP-Based Genetic Linkage Map of Channel catfish (Ictalurus punctatus) Constructed by Using an Interspecific Hybrid Resource Family. Genetics 165, 687-694.

Mickett, K., Morton, C., Feng, J., Li, P., Simmons, M., Dunham, R.A., Cao, D., and Liu, Z.J. 2003. Assessing genetic diversity of domestic populations of channel catfish (Ictalurus punctatus) in Alabama using AFLP markers. Aquaculture 228, 91-105.

Patterson, A., Karsi, A., Feng, J., and Liu, Z. J. 2003. Translational Machinery of Channel Catfish: II. Complementary DNA and Expression of the Complete Set of 47 60S Ribosomal Proteins. Gene 305, 151-160.

Reece, K.S, Ribeiro, W.L., Gaffney, P.M., Carnegie, R.B. and Allen, S.K. Jr. Microsatellite marker development and analysis in the eastern oyster, Crassostrea virginica: Confirmation of null alleles and non-Mendelian segregation ratios. J. Heredity (in press).

Streelman, J.T. and T.D. Kocher. 2002. Microsatellite length variation is associated with prolactin expression and growth response of salt-challenged tilapia. Physiological Genomics 9:1-4.

Yu, Z. and X. Guo. 2003. Genetic linkage map of the eastern oyster Crassostrea virginica Gmelin. Biol. Bull. 204: 327?338.

Cattle:

Adelson, D.L., Cam, G.R., DeSilva, U. and Franklin, I.R. (2004) Gene Expression in Sheep Skin and Wool (Hair).Genomics 83 (1): 95-105.

Agca, C., C.A. Bidwell, and S.S. Donkin. (In Press) Cloning of bovine pyruvate carboxylase and 5‘‘‘‘ untranslated region variants. Animal Biotechnology.

Ashwell, M. S., D. W. Heyen, T. S. Sonstergard, C. P. Van Tassell, Y. Da, P. M. Van Raden and H. A. Lewin. 2004. Detection of quantitative trait loci affecting female fertility and milk production in ten Dairy Bull DNA Repository families. J. Dairy Sci. (In press)

Brunner, R.M., Sanftleben, H., Goldammer, T., Kühn, C., Weikard, R., Kata, S.R., Womack, J.E., and Schwerin, M.: The telomeric region of BTA18 containing a potential QTL region for health in cattle exhibits high similarity to the HSA19q region in humans. Genomics, 81:270-278, 2003.

Chowdhary, B.P., Raudsepp, T., Kata, S.R., Goh, G., Millon, L.V., Allan, V., Piumi, F., Guérin, G., Swinburne, J., Binns, M., Lear, T.L.. Mickelson, J., Murray, J., Antczak, D.F., Womack, J.E., and Skow, L.C.: The first-generation whole-genome radiation hybrid map in the horse identifies conserved segments in human and mouse genome. Genome Research, 1:742-751, 2003.

Denniston, D.J., M.G. Thomas, K.K. Kane, C.N. Roybal, L. Canales, D.M. Hallford, M.D. Remmenga, and D.E. Hawkins. 2003. Effect of Neuropeptide Y on GnRH-induced LH release from bovine anterior pituitary cell cultures derived from heifers in a follicular, luteal, or ovariectomized state. Anim. Reprod. Sci. 78:25-31.

DeSilva, U, I.R. Franklin, B. van Hest, J.F. Maddox and D.L. Adelson Systematic screening of sheep skin cDNA libraries for microsatellite sequences. J. Cytogenetics and Genome Research (in press).

Eun-Joon Lee, Terje Raudsepp, Srinivas R. Kata, David L. Adelson, James E. Womack, Loren C. Skow, Bhanu P. Chowdhary. A 1.4 Mb interval RH map of horse chromosome 17 provides detailed comparison with human and mouse homologues. Genome Research (In press).

Farber, C.R. and J. F. Medrano 2003. In silico anchoring of ESTs, genes and genomic sequence to bovine, porcine and chicken genome maps using SSLP markers. Animal Genetics 34:11-18.

Farber, C.R. and J.F. Medrano 2004. Identification of putative homology between horse microsatellite flanking sequences and cross-species ESTs, mRNAs and genomic sequences. Animal Genetics (In press)

Hamernik DL, Adelson DL (2003) USDA stakeholder workshop on animal bioinformatics: summary and recommendations. Comparative And Functional Genomics, 4 (2): 271-274.

Hamernik, D. L., H. A. Lewin, and L. B. Schook. 2003. Allerton III. Beyond Livestock Genomics. Animal Biotechnology 14:77-82.

Kim, K.-S., J.M. Reecy, W.H. Hsu, L.L. Anderson, and M.F. Rothschild. 2003. Functional and phylogenetic analyses of a melanocortin-4 receptor mutation in domestic pigs. Domestic Animal Endocrinology (In Press).

Krum, S.A., Womack, J.E., and Lane, T.F.: Bovine BRCA1 shows classic responses to genotoxic stress but low in vitro transcriptional activation activity. Oncogene, 22:6032-6044, 2003.

Kurar, E., J.E. Womack and B.W. Kirkpatrick. 2003. A radiation hybrid map of bovine chromosome 24 and comparative mapping with human chromosome 18. Animal Genetics 34:198-204.

Larkin, D. M., A. Everts-van der Wind, M. Rebeiz, P. A. Schweitzer, S. Bachman, C. Green, C. L. , Wright, E. J. Campos, L. D. Benson, J. Edwards, L. Liu, K. Osoegawa, J. E. Womack, P. de Jong, and H. A. Lewin. 2003. A cattle-human comparative map built with cattle BAC-ends and human genome sequence. Genome Research 13: 1966-1972.

Larkin, D.M., der Wind, A.E., Rebeiz, M., Schweitzer, P.A., Bachman, S., Green, C., Wright, C.L., Campos, E.J., Benson, L.D., Edwards, J., Liu, L., Osoegawa, K., Womack, J.E., de Jong, P., and Harris, A.L.: A cattle-human comparative map built with cattle BAC-ends and human genome sequence. Genome Research 13:1966-1973, 2003.

Lewin, H. A. 2004. The future of cattle genomics: the beef is here. Cytogenetics and Genome Research (in press)

McKay, S.D., White, S.N., Kata, S.R, Loan, R., and Womack, J.E.: The bovine 5? AMPK gene family: mapping and single nucleotide polymorphism detection. Mammalian Genome, 14:853-858, 2003.

Mishra, B.P. and J. M. Reecy. 2003.Mutations in the limbin gene previously associated with dwarfism in Japanese brown cattle are not responsible for dwarfism in the American Angus breed. Animal Genetics 34(4):311-2

Moody, D.E., A.J. Rosa, and J.M. Reecy. 2003. Current status of livestock DNA microarrays. AgBioTech (In Press).

Morrison, C.D., J.A. Daniel, J.H. Hampton, P.R. Buff, T.M. McShane, M.G. Thomas, and D.H. Keisler. 2003. Regulation of luteinizing hormone and growth hormone in ewes treated with Neuropeptide Y. Domestic Anim. Endocrinol. 24:69-80.

Narro, L.A. , M.G. Thomas, G.A. Silver, K.J. Rozeboom, and D.H. Keisler. 2003. Body composition, leptin, and the leptin receptor and their relationship to the growth hormone (GH) axis in wethers treated with zeranol. Domest. Anim. Endocrinol. 24:243-255.

Potts, J.K., S.E. Echternkamp, T.P.L. Smith, and J.M. Reecy. 2003. Characterization of Gene Expression In Double-Muscled and Normal-Muscled Bovine Embryos. Animal Genetics 34, 438?444

Schwerin, M., Czernek-Schafer, D., Goldammer, T., Kata, S.R., Womack, J.E., Pareek, R., Pareek, C., Krzysztof, W., and Brunner, R.M.: Application of disease-associated differentially expressed genes - Mining for functional candidate genes for mastitis resistance in cattle. Genet. Sel. Evol., 35:S19-S34, 2003.

Soller, M. and J.M. Reecy. 2003. QTL Mapping and Cloning in Beef Cattle. AgBioTech (In Press).

White, S.N., Kata, S.R., and Womack, J.E.: Comparative fine maps of bovine toll-like receptor 4 and toll-like receptor 2 regions. Mammalian Genome, 14:149-155, 2003.

White, S.N., Taylor, K.H., Abbey, C.A., and Gill, C.A., and Womack, J.E.: Haploytype viaration in bovine Toll-like receptor 4 and computational predication of a positively selected ligand-binding domain. Proc. Natl. Acad. Sci. USA 100:10364-10369, 2003.

Horse:

Ainsworth, D.M., Appleton, J.A., Eicker, S.W., Luce, R., Flaminio, J.B.F., Antczak, D.F. (2003a) The effect of strenuous exercise on mRNA concentration of interleukin-12, interferon-gamma and interleukin-4 in equine pulmonary and peripheral blood mononuclear cells. Vet Immunol. Immunopath. 91:61-71.

Ainsworth, D.M., Grunig, G., Matychak, M.B., Young, J., Wagner, B., Erb, H.N., and Antczak, D.F. (2003b) Recurrent airway obstruction (RAO) in horses is characterized by IFN-g and IL-8 production in bronchoalveolar lavage cells. Vet Immunol. Immunopath. 96:83-91.

Bryan, T.M., C.A. Abbey, T. Raudsepp, B.P. Chowdhary, C.A. Gill, T.L. Blanchard, N.H. Ing and T.H.Welsh, Jr. ( 2003) Characterization of equine bacterial artificial chromosomes (BACs) relevant to endocrine and immune system regulation. J. Anim. Sci. 81(Suppl.1):102-103.

Chowdhary, B.P., Raudsepp, T., Kata, S.R., Goh, G., Millon, L.V., Allan, V., Piumi, F., Guérin, G., Swinburne, J., Binns, M., Mickelson, J., Murray, J., Antczak, D.F., Womack, J.E., Skow, L.C. (2003) The first generation whole genome radiation hybrid map in the horse identifies conserved segments in human and mouse genomes. Genome Research 13:742-751.

Chowdhary B.P. & Bailey E. (2003) Equine Genomics: Galloping to new frontiers. Cytogenetics & Genome Research, in press.

Curik,I., Fraser,D., Eder,C., Achmann,R., Swinburne,J., Binns,M., Crameri,R., Brem,G., Sölkner,J. and Marti,E. (2003) Association between the MHC gene region and variation of serum IgE levels against specific mould allergens in the horse. Genetics Selection Evolution 35: S177-S190.

Gallagher PC, Morrison S, Bernoco D & Bailey E. (2003) Measurement of back curvature in American Saddlebred horses: structural and genetic basis for early-onset lordosis. The Journal of Equine Veterinary Science 23: 71-76.

Guérin, G., Bailey, E., Bernoco, D., Anderson, I., Antczak, D.F., Bell, K., Biros, I., Bowling, A.T., Brandon, R., Cholewinski, G., Colling, D., Eggleston, M., Flynn, J., Gralak, B., Hasegawa, T., Ketchum, M., Lindgren, G., Lyons, L., Millon, L.V., Mariat, D., Murray, J., Neau, A., Røed, K., Sandberg, K., Skow, L.C., Tammen, I., Van Dyk, E., Weiss, B., and Ziegle, J. (2003) The Second Iteration of the International Equine Gene Mapping Workshop: Half-Sibling Linkage Map. Animal Genetics 34:161-168.

Gustafson A.L., Tallmadge R.L., Ramlachan N., Miller D., Bird H., Antczak D.F., Raudsepp T., Chowdhary B.P., and Skow L.C. (2003) An ordered BAC Contig Map of the Equine Major Histocompatibility Complex. Cytogenetics & Genome Research (in press).

Hamernik DL, Adelson DL (2003) USDA stakeholder workshop on animal bioinformatics: summary and recommendations. Comparative And Functional Genomics, 4 (2): 271-274.

Lee, E-J, Raudsepp T., Kata S.R., Adelson D., Womack J.E., Skow L.C., Chowdhary B.P. (2003) A 1.4 Mb interval RH map of horse chromosome 17 provides detailed comparison with human and mouse homologues. Genomics (in press).

Mariat D, Taourit S, Guérin G. (2003) A mutation in the MATP gene is associated to the cream coat colour in the horse. Genetics, Selection and Evolution 35:119-133.

Marti, E., Horohov, D.W., Antczak D.F., Lazary S., Lunn D.P. (2003) Advances in equine immunology: Havemeyer workshop reports from Santa Fe, New Mexico, and Hortobagy, Hungary. Vet. Immunol. Immunopath. 91:233-43.

Mickelson, J.R., Wu J.T., Morrison L.Y., Swinburne J.E., Binns M.M., Reed K.M., Alexander L.J. (2003) Eighty-three previously unreported equine microsatellite loci. Animal Genetics 34: 71-74.

Milenkovic D, Chaffaux St, Taourit S, Guérin G. (2003) A mutation in the LAMC2 gene causes the Herlitz junctional epidermolysis bullosa (H-JEB) in two French draft horse breeds. Genetics, Selection and Evolution 35: 249-256.

Mousel M.R., Harrison L., Donahue M. and E. Bailey (2003) Molecular typing of transferrin from paraffin embedded tissues of thoroughbred foals that succumbed to Rhodococcus equi. Vet Lab Investigation 15:470-472.

Myka JL, Lear TL, Houck ML, Ryder OA, Bailey E (2003). FISH analysis comparing genome organization in the domestic horse (Equus caballus) to that of the Mongolian Wild horse (E. przewalskii). Cytogenetic and Genome Research (in press).

Myka JL, Lear TL, Houck ML, Ryder OA, Bailey E (2003). Homologous fission event(s) implicated for chromosomal polymorphisms among 5 species in the genus Equus. Cytogenetic and Genome Research (in press).

Raudsepp T., Lee E.J., Kata S., Brinkmeyer C., Mickelson J.R., Womack J.E., Skow L.C., Chowdhary B.P. (2003). Exceptional conservation of horse human gene order on X chromosome revealed by high resolution radiation hybrid mapping. PNAS (in press).

Swinburne J.E., Turner A., Alexander L.J., Mickelson J.R., and Binns M.M. (2003). Characterization and linkage map assignments for 61 new horse microsatellite loci (AHT49-109). Animal Genetics 34, 65-68.

Takahashi, T., Yawata, M., Raudsepp, T., Lear, T. L., Chowdhary, B. P., Antczak, D. F., and Kasahara, M. Natural killer cell receptors in the horse: evidence for the existence of multiple transcribed LY49 genes. European Journal of Immunology, in press.

Tallmadge, R.L., Lear, T.L., Johnson, A.K., Guérin, G., Millon, L.V., Carpenter, S.L. and Antczak, D.F. (2003) Characterization of the ??-2-microglobulin gene of the horse. Immunogenetics 54:725-733.

Tozaki T, Takezaki N, Hasegawa T, Ishida N, Kurosawa M, Tomita M, Saitou N, and Mukoyama H. (2003) Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup. Journal of Heredity 94(5): 374-80.

Wagner, B., Greiser-Wilke, I., and Antczak, D.F. (2003) Characterization of the horse (Equus caballus) IGHA gene. Immunogenetics 55:552-560.

Wagner M., Goh G., Wu J.T., Raudsepp T., Morrison L.Y., Alexander L.J., Skow L.C., Chowdhary B.P., Mickelson J.R. (2003). Radiation hybrid mapping of 75 previously unreported equine microsatellite loci. Animal Genetics, in press.

Wagner M, Goh G, Wu JT, Raudsepp T, Morrison LY, Alexander LJ, Skow LC, Chowdhary B.P., Mickelson J.R. (2003). Radiation hybrid mapping of 65 previously unreported equine microsatellite loci. Animal Genetics, submitted.

Ward TL, Valberg SJ, Lear T, Guerin G, Milenkovic D, Swinburne J, Binns MM, Chowdhary BP, Raudsepp R, Skow S and Mickelson JR (2004) Genetic mapping of GBE1 and its association with glycogen storage disease IV in American Quarter Horses. Cytogenetics and Genome Research, in press.

Ward TL, Valberg SJ, and Mickelson JR Glycogen branching enzyme (GBE1) mutation causing fatal glycogen storage disease IV in American Quarter Horse foals. Mammalian Genome, submitted.

Ward TL, Valberg SJ, Lear TL, Guerin G, Milenkovic D, Swinburne J, Binns MM, Chowhary BP, Raudsepp T, Skow L, Mickelson JR (2003). Mapping of GBE1 and flanking human chromosome 3p11-p12 genes to equine chromosome 26. Cytogenetic and Genome Research, in press.

Sheep:

Agca, C., C.A. Bidwell, and S.S. Donkin (2004) Cloning of bovine pyruvate carboxylase and 5‘‘‘‘ untranslated region variants. Anim. Biotech. (in press).

Cockett, N. E. (2003) The genetic mutation responsible for spider lamb syndrome in sheep. Suffolk News 9(45):25.

Cockett, N. E. (2003) Current status of the ovine genome map. Cytogenetic and Genome Research (in press).

Cockett, N. E. (2003) Radiation hybrid mapping and its application to the ovine genome. INRA Informer, May 2003.

Cockett, N. E., C. A. Bidwell, C. Charlier, M. Smit, K. Sergers, T. L. Shay, L. Karim, G. D. Snowder and M. Georges (2003) Muscle enhanced traits in sheep. Progess in Obesity Research 9:272-274.

Farber, C.R. and J. F. Medrano (2003) In silico anchoring of ESTs, genes and genomic sequence to bovine, porcine and chicken genome maps using SSLP markers. Anim. Genet. 34:11-18.

Georges, M., C. Charlier and N. E. Cockett (2003) Polar overdominance at the ovine callipyge locus supports trans interaction between the products of reciprocally imprinted genes. Trends in Genetics 19:248-252.

Guo, X., Kupfer, D. M., Fitch, G. Q., Roe, B. A., and DeSilva, U (2003) Identification of a novel lysine- 171 allele in the ovine prion protein (PRNP) gene. Anim.Genet. 34:303-305.

Notter, D. R., N. E. Cockett and T. L. Shay (2003) Evaluation of melatonin receptor 1a as a candidate gene influencing reproduction in an autumn-lambing sheep flock. J. Anim. Sci. 81:912-917.

Smit, M., K. Segers, T. Shay, F. Baraldi, G. Gyapay, G. Snowder, M. Georges, N. Cockett and C. Charlier (2003) Mosaicism of Solid Gold supports the causality of the SNP in determinism of the callipyge phenotype. Genetics 163:453-456.

DeSilva, U*., Guo, X., Kupfer, D. M., Fernando, S. C., Pillai, A. T. V., Najar, F. Z., So, S., Fitch, G. Q., and Roe, B. A. (2004) Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep. (In press).

Farber, C.R. and J.F. Medrano (2004) Identification of putative homology between horse microsatellite flanking sequences and cross-species ESTs, mRNAs and genomic sequences. Anim. Genet. (In press).

Laughlin, A. M., D. F. Waldron, B. F. Craddock, G. R. Engdahl, R. K. Dusek, J. E.
Huston, C. J. Lupton, D. N. Ueckert, T. L. Shay and N. E. Cockett (2004) Use of
DNA markers to determine paternity in a multiple-sire mating flock. Sheep and
Goat Res. J. (In press).

Swine:

Bannerman, D., Paape, M.J., Lunney, J.K. 2004.Immunity: Innate. In Encyclopedia of Animal Science; Pond, W.G., Bell, A.W. Eds.; Marcel Dekker, Inc., New York.

Bertani G, Gladney C, Johnson RK, Pomp D (2004) Evaluation of gene expression in pigs selected for enhanced reproduction. II: Anterior Pituitary. J Anim Sci (In Press).

Bertani G, Johnson RK, Pomp D (2003) Mapping of porcine ESTs putatively differentially expressed in anterior pituitary. Animal Genetics 34:132-134.

Bidwell, C. A., M. Spurlock, and D. E. Moody. 2003. Serial analysis of gene expression of pig adipose and muscle tissue. Plant and Animal Genome Conference XI, San Diego, CA. January, 2003. www.intl-pag.org/11/abstracts/P01_P69_XI.html

Blowe, C. D., E. J. Eisen, O. W. Robison, and J. P. Cassady. 2003. Characterization of a line of pigs selected for increased litter size for genes known to affect reproduction. Abstract. Accepted. American Society of Animal Science, Midwest Sectional Meetings.

Caetano A, RK Johnson, D Pomp (2003) Generation and Sequence Characterization of a Normalized cDNA Library from Swine Ovarian Follicles. Mammalian Genome 14:65- 70.

Chaiwong, N., J.C.M. Dekkers, R.L. Fernando and M.F. Rothschild. 2002. Introgressing Multiple QTL through Backcross Breeding Programs. Swine Research Reports. Iowa State University.

Ciobanu, D.C., S.M. Lonergan, M. Malek, J.R. Woollard, E.J. Lonergan and M.F. Rothschild. 2002. New Alleles in the Calpastatin Gene Associated with Improved Tenderness in Pigs. Swine Research Reports. Iowa State University

Dawson H., Nishi S., Beshah E., Solano-Aguilar G., Zarlenga D., Urban J.F., Lunney J.K. 2002. Functional genomic assessment of porcine disease resistance: real time assays of porcine immune gene expression. Proceedings 7th World Congress on Genetics Applied to Livestock Production. #13-41.

Dawson, Harry, Sandra Nishi, Ethiopia Beshah, Gloria Solano-Aguilar, Dante Zarlenga, Joseph F. Urban, Joan Lunney. 2003. Use of real-time assays of immune gene expression to assess the genetic basis of disease resistance. http://www.intl-pag.org/11/abstracts/W52_W330_XI.html

Ernst, C.W., N.E. Raney, V.D. Rilington, G.A. Rohrer, J.A. Brouillette and P.J. Venta. 2004. Mapping of the FES and FURIN genes to porcine chromosome 7. Anim. Genet. In Press.

Farber, C.R., N.E. Raney, D.L. Kuhlers, K. Nadarajah and C.W. Ernst. 2003. Identification of genetic markers between two pig populations using representational difference analysis. Anim. Biotech. 14:87-102.

Farber, C.R., N.E. Raney, V.D. Rilington, P.J. Venta and C.W. Ernst. 2004. Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig. Cytogenet. Genome Res. In Press.

Flaherty L, O Abiola, J Angel, P Avner, A Bachmanov, J Belknap, B Bennett, E Blankenhorn, D Blizard, V Bolivar, G Brockmann, K Buck , J Bureau, W Casley, E Chesler, J Cheverud, G Churchill, M Cook, J Crabbe, W Crusio, A Darvasi, G de Haan, P Demant, R Doerge, R Elliott, C Farber, J Flint, H Gershenfeld, J Gibson, W Gu, H Himmelbauer, R Hitzemann, HC Hsu, K Hunter, F Iraqi, R Jansen, T Johnson, B Jones, G Kempermann, F Lammert, L Lu, K Manly, D Matthews, J Medrano, M Mehrabian, G Mittleman, B Mock, J Mogi, X Montagutelli, G Morahan, J Mountz , H Nagase, R Nowakowski, B O‘‘‘‘Hara, A Osadchuk, B Paigen, A Palmer, J Peirce, D Pomp, M Rosemann, G Rosen, L Schalkwyk, Z Seltzer, S Settle, K Shimomura, S Shou, J Sikela, L Siracusa, J Spearow, C Teuscher, D Threadgill, L Toth, A Toye, C Vadasz, G Van Zant, E Wakeland, R Williams, H-G Zhang, Fei Zou (2003) The nature and identification of quantitative trait loci: a community?s view. Nature Genetics Reviews 4:911- 916.

Gladney C, G Bertani, MK Nielsen, D Pomp (2004) Evaluation of gene expression in pigs selected for enhanced reproduction. I: Ovarian Follicles. J Anim Sci (In Press).

Jiang, Z. and J.J. Michal. 2003. Linking porcine microsatellite markers to known genome regions by identifying their human orthologs. Genome 46:798-808.

Jiang, Z., M. Zhang, V.D. Wasem, J.J. Michal, H. Zhang and R.W. Wright Jr. 2003. Census of genes expressed in porcine embryos and reproductive tissues by mining EST database based on the human genes. Biology of Reproduction 69:1177-1182.

Jiang, Z., Xiao-Lin Wu, Ming Zhang and Raymond W. Wright Jr. (2003) In Silico Gene Expression Profiling of Basic Active Genes (BAGs) in Porcine Embryos and Reproductive tissues using a comparative gene-based approach. Computational Biology and Genome Information, Cary, North Carolina, USA.

Kim, K.S., N. T. Nguyen, J. M Reecy, L. L. Anderson, and M. F. Rothschild. 2002. Molecular genetic studies of porcine genes for obesity. Swine Research Reports. Iowa State University.

King, A.M., Z. Jiang, J.P. Gibson, C.S. Haley and A.L. Archibals. 2003. Mapping Quantitative Trait Loci Affecting Female Reproductive Traits on Porcine Chromosome 8. Biology of Reproduction 68: 2172-2179.

Lunney, J.K. 2003. Are there immune gene alleles that determine whether a pig will be healthy? Genetics of Pig Health Symposium, Des Moines, IA.

Lunney, J.K. 2003. In Search of Disease-Resistant Pigs. National Hog Farmer, Apr 15, 2003, pp.30-34.

Lunney, J.K., Paape, M.J., Bannerman, D. 2004. Immunity: Acquired. In Encyclopedia of Animal Science; Pond, W.G., Bell, A.W. Eds.; Marcel Dekker, Inc., New York.

Malek, M., D. C. Ciobanu, and M. F. Rothschild. 2003. Genetic and physical mapping of ACACB, PPP1CC and GPR49 genes on porcine chromosomes 5 and 14 help to define a break point on human chromosome 12. Proceedings Plant & Animal Genome XI meeting, January 11-15, San Diego, CA.

Martens G W, Lunney J K, Baker J E, Smith D M. 2003. Rapid Assignment of Swine Leukocyte Antigen (SLA) Haplotypes in Pedigreed Herds using a Polymerase Chain Reaction Based Assay. Immunogenetics. 55: 395-401.

N.-T. Nguyen, K.-S. Kim, H. Thomsen, J. Helm and M. F. Rothschild 2003. Investigation of candidate gene for growth and fatness QTL on the pig chromosome 7. Proceedings Plant & Animal Genome XI meeting, January 11-15 San Diego, CA.

Nishi, S., Dawson, H., Dubey, J.P., Urban, J.F., Lunney, J. 2003. Immune responses controlling Toxoplasma gondii infection in pigs. Proceedings of the American Association of Veterinary Parasitologists, 2003. v.48. Abstract. p.52.

Otieno, C.J., S. Vleck, C. Jelks, K.-S. Kim, N. T. Nguyen and M. F. Rothschild. 2003. Mapping of diabetes-related genes in the pig. Proceedings Plant & Animal Genome XI meeting, January 11-15, San Diego, CA.

Pan, P.-W., K. Li, C.K. Tuggle, M. Yu, B. Liu, and S.-H. Zhao. 2003. Sequencing, tissue distribution and physical mapping of the porcine homologue of cardiomyopathy associated 3 (CMYA3). Anim. Genet. 34:1-4.

Patil, A., Y. Sang, K. Rune, F. Blecha, J.E. Minton, and G. Zhang. 2003. Porcine Innate Immunity: Distinct Tissue Expression and Transcriptional Regulation Patterns of Porcine Beta-Defensin-1 and ?2. Proceedings of the 84th Annual Meeting of the Conference of Research Workers in Animal Diseases (CRWAD), Chicago, IL.

Pomp D, Allan MF, Wesolowski S. Quantitative Genomics: Exploring the genetic architecture of complex trait predisposition. J Anim Sci (In Press).

Risatti GR, D Pomp, RO Donis (2003) Patterns of cellular gene expression in cells infected with cytopathic or non-cytopathic bovine diarrhea virus. Animal Biotechnology 14:31-49.

Rocha J, Eisen EJ, Van Vleck DL, Pomp D (2004) A large sample QTL study in mice. I: Growth. Mammalian Genome (In Press)

Rocha J, Eisen EJ, Van Vleck DL, Pomp D (2004) A large sample QTL study in mice. II: Body Composition. Mammalian Genome (In Press)

Rothschild, M.F. 2003. Advances in pig genomics and functional gene discovery. Comp. Funct. Genom. 4:266-277.

Rothschild, M.F. 2003. Approaches and Challenges in Measuring Genetic Diversity in Pigs. Archivos de Zootecnia 52:129-135

Thomsen, H., J. C. M. Dekkers, and M. F. Rothschild. 2002. Detection and characterization of QTL for growth and meat quality traits in the Berkshire-Yorkshire cross. Swine Research Reports. Iowa State University

Tuggle C, Green JA, Fitzsimmons C, Woods R, Prather RS, Malchenko S, Soares MB, Tack D, Robinson N, O‘‘‘‘Leary B, Scheetz T, Casavant T, Pomp D, Edeal JB, Zhang Y, Rothschild MF, Garwood K, Beavis W (2003) EST-Based Gene Discovery in Pig: Virtual Expression Patterns and Comparative Mapping to Human. Mammalian Genome 14:565-579.

Tuggle, C.K., J. A. Green, C. Fitzsimmons, R. Woods, R. S. Prather, S. Malchenko, M. B. Soares, D. Tack, N. Robinson, B. O‘‘‘‘Leary, T. Scheetz, T. Casavant, D. Pomp, J. B. Edeal, Y. Zhang, M. F. Rothschild, K. Garwood, W. Beavis .2003. EST-Based Gene Discovery in Pig: Virtual Expression Patterns and Comparative Mapping to Human, Mammal. Genome 14:565-579

Walker, S. E., O.W. Robison, C.S. Whisnant, and J.P. Cassady. Effect of selection for testosterone production on testicular morphology and daily sperm production in pigs. J. Anim Sci.. 81(Suppl. 1), 23 Jun 2003

Wesolowski S, Allan MF, Nielsen MK, Pomp D (2003) Evaluation of hypothalamic gene expression in mice selected for heat loss. Physiological Genomics 13:129-137.

Wesolowski, S.R., N.E. Raney and C.W. Ernst. 2004. Developmental changes in the fetal pig transcriptome. Physiol. Genomics. In Press.

Zhao, H., M. F. Rothschild, R. L. Fernando, J. C.M. Dekkers 2003. Tests of candidate genes in QTL mapping resource populations. Mammal. Genome 14:472-482.

Zhao, S.-H., D. Nettleton, W. Liu, C. Fitzsimmons, C.W. Ernst, N.E. Raney, and C.K. Tuggle. 2003. Complementary DNA macroarray analyses of differential gene expression in porcine fetal and postnatal muscle. J. Anim. Sci. 81:2179-2188.

Zhao, S-H., D. Nettleton, W. Liu, C.W. Fitzsimmons, C.W. Ernst, N.E. Raney and C.K. Tuggle. 2003. Complementary DNA macroarray analysis of differential gene expression in porcine fetal and postnatal muscle. J. Anim. Sci. 81:2179-2188.