Brief Summary of Minutes of Annual Meeting

Jan. 23-24, 2013

Wyndham Riverfront Hotel 701 Convention Center Blvd. New Orleans, LA 70130 (866) 907-0611 www.wyndhamriverfront.com

Wednesday, January 23rd

1:30 - Welcome: Tom Hunt

Nomination committee: Established to select new secretary/chair-elect. Secretary will start at Jan. 2014 meeting and continue as secretary until assuming Chair role for five-year term following Tom Hunts term. Tom Sappington was nominated and accepted as secretary and chair-elect. Tom Hunt chairship ends 09/30/15 (assuming continued yearly election), new chair (Tom Sappington) will begin 10/01/15 as new NC-205 Chair.

Time and Place: Next annual meeting (with NCCC46 and joint meeting) will be January 27-30, 2014. Atlanta, GA. (David Buntin, local arrangements). NC-205 will be held the 27th-28, 2014.

International Working Group on Ostrinia (IWGO) with European scientists will be held separately, April, 2014 in Chicago (Dates to be determined).

Administrative Advisor Report (Steve Pueppke): NC205 is in mid-term review process in the 5-year cycle. NCRA administrative advisors will meet March 2013 to review the current project progress. Two things are required of NC205 very soon: 4-5 impact statements and reports of NC-205 leverage ( external funding and collaborations).

External funding leverage and collaboration report can be included with annual report from New Orleans meeting, due within 60 days of meeting.

Project re-write will be due 2014, with review in 2015.

Recap of Activities and Influence: Iowa (Gassmann): Discussed rootworm issues with Iowa Corn Growers Association.

Wisconsin (Cullen): Invited presentations on IRM and NC205/NCCC46 joint projects (and NCRA Directors response) to ECOP/ESCOP National IPM Committee, Washington, DC, UW-Madison Evolution Seminar Series, and extension meetings fall 2012 and winter 2013 reaching 500+ ag professionals.

Michigan/Wisconsin (DiFonzo/Cullen): Handy Bt Trait Table utilized nationally.

Minnesota (Andow): Involved with radio interview and call-in Q&A with general public on transgenic crop regulation and safety, invited to present to Papal Council on Justice and Peace, Vatican City on biotechnology and the rural poor. Attended Malaysia conference (see UMN newsletter).

Texas (Porter/Bynum): Had discussions with TX corn promotion board. Amarillo is dividing line between corn and cotton refuge requirements. Refuge in strips 6-8 rows for CEW and FAW protection south of Amarillo in cotton region will be recommended . This is for pyramids only. Data suggests ¼ or less damage than youd expect in larger blocks, but it will drive resistance more quickly.

Nebraska (Siegfried): Worked with ABSTC on IRM monitoring. Attended conference in the Philippines to mark a decade of commercialization of Bt corn. Blair spoke on IRM and regulation topics.

Maryland (Divley): Worked with Syngenta on cross-pollination from Bt to refuge corn and impact on corn earworm. Had conversations with Monsanto on USDA BRAG project on aquatic risk assessment (Bt). Stream runoff risk negligible. Tissue analysis after harvest found long residual of Bt in plant tissues, very little residue in Cry1Ab, but up to 5 mos. in SmartStax as measured by ECB bioassay in 7 day assay. Presented brown marmorated stink bug in corn talks, honey bee and seed treatment talks, etc.

Delaware (Mason): Wilmington News Journal, article. Includes IRM and NC205 research in college course content.

USDA ARS, Ames, IA (Hellmich): presented non-target organism work and committee service with international audience. Organized ESA symposium on international GMO regulation. Gave invited presentation to China Agricultural University on Bt corn and ECB and CRW. Planning a workshop in Ames summer 2013 with 15 scientists from Africa field and lab tests on non-target organisms. Trying to influence regulatory policy in Africa and Asia.

USDA ARS, Ames, IA (Sappington): Organized a symposium at the international congress of entomology in South Korea. Gave invited talk to Chinese Academy of Sciences on Lepidoptera flight mill work.

New York: Elson Shields received IPM award, Eastern branch ESA.

North Dakota (Knodel): Pheromone trap network for ECB in non-Bt area of northern ND indicated of E and Z strains, majority are Z strains. Also trapped SW North Dakota and picked up ECB, surprised at seasonal numbers of 300-400 moths.

Pennsylvania (Tooker): True armyworm outbreak 2012 garnered media and PA Dept. of Ag. attention (first and second generations a problem). Brown marmorated stink bug was found in corn and garnered lots of media attention. Co-organized 2012 ESA symposium on IPM and transgenic crops and seed treatments. TAW mostly in small grains and hay.

Nebraska (Hunt): Western bean cutworm binomial sampling plan is available at the Apple App store (free).

Guelph, Canada (Smith): True armyworm outbreak in spring 2012 on small grains, but 2nd generation was suppressed by parasitism.

Ohio (Michel): Brown marmorated stink bug in corn. This was a year of miscellaneous odd pests. Suggestion for future NC205 (and NCCC46) project to incorporate non-lep pests like stink bugs in corn.

Louisiana (Huang): Invited to China to speak about transgenic crops in USA, gave ESA presentation on FAW in the south, resistance to Cry1F, and an EPA requested talk. There was internal EPA discussion/review of this work. Invited industry talks on FAW Cry1F resistance were given. Reported benefits of planting Bt corn to LA Corn Promotion Board. ECB not widespread in LA. Received two LSU awards.

NCR 327 Progress: (Mason) Requires $25-30K to complete publication. Working through ISU to publish with Erin Hodgon. Need to find funding up front, which is a challenge. Regional IPM Center contributions may be pursued. Another possibility is for NC-205 members (each experimental station) to put up about 2K each, with some possibility of return from sales profit. If/when money found, need text review again. Pat Porter offered to try iBooks Author, self-publishing e-book format, easier than In Design.

Tom Hunt (and Mason) will call S, NC, and E IPM Center Directors re: 205 publication support.

Invited presentations: Holly Johnson (Mason Lab) - Presentation on FAW larval movement on Bt Corn 1. Determine diff. early and late instar movement and survival. 2. Characterize larval injury to plants. A DuPont/Pioneer, Univ. of NE, Univ of Delaware, ISDA (Ames IA) collaboration (Hellmich, Mason, Hunt) Results: Late instars were rarely recovered across entries. Greatest from pure-stand refuge, and clusters where they started on refuge. Most of larvae were recovered from center infested plant. Also see a dispersal from center refuge plant. Feeding damage combined (not early and late instars). Conclusions (preliminary): Pure stand refuge had greater percent recovery on adjacent plants by late instars, fewer when Bt were central plants (Most from central egardless). Transgenic plants adjacent to the refuge had minor to no damage.

Fei Yang (PhD LSU, Huang Lab)- Presentation Effect of pollen contamination on larval survival, pupation, and plant injury on corn earworm in seed blends of Bt and non-Bt corn. 80% of kernels in non-Bt refuge were expressing Bt toxin. CEW does not have oviposition preference between Bt and non-Bt plants. No sig. diff in kernel damage between non-Bt and RIB non-Bt plants. No sig. diff kernel damage between pure non-Bt and RIB non-Bt plants (nor eggs/silk, larvae/ear, cm damage). Development of CEW on RIB non-Bt was delayed compared to that on pure non-Bt plants. Also did a field study with SmartStax and isoline.

Pat Porter - Compared FAW and CEW in refuge blends and to non-Bt corn 12 row away (strips). Seed blends had far fewer live larvae recovered from non-Bt ears, far less damage on non-Bt ears, significantly less tip damage (CEW+FAW), numerically less lower ear damage (FAW).

Are Yield Differences in Blends Only Due to Direct Insect Damage? There is sig. damage in whorl stage corn, which disadvantages damaged plant (competition). Neighbors are most often undamaged Bt plants.

5:00 - Adjourn for day.

Thursday, January 24, 2013

8:00 - 2012 Annual meeting minutes were approved. Tom Hunt was nominated and elected to continue as Chair for 2013 on current project.

State Reports: State Reports and Discussion, by Objective: Research, extension, and outreach reports were presented by state representatives to NC-205. Discussion ranged over a variety of NC-205 objective-related topics.

Other Discussion: (Tooker) - Leland Glenna PSU, rural sociologist requested to survey members of the group (and NCCC-46) with respect to interaction with Industry. NCCC-46 asked that Leland write up a list of questions and objectives for March meeting. He takes a non-partisan approach, including industry biotech companies. NC205 committee is supportive of idea. Tom Hunt will send questions and reprints of previous articles to group.

Chuck Mason  Introduced the idea of insect reserve (public variety of corn planted in each location) where you could go to find populations not affected by Bt proteins. Is there support from the group, does this make good scientific sense?

Galen Dively  depends on what your objectives are. Non-Bt sweet corn gives you a good sweet corn resistance development screen. Think about it for this year, maybe revisit in the future.

3:00 ADJOURN 2013 NC-205 Annual Meeting.

Illinois Cooperative Ag Pest survey coordinator is monitoring BMSB interceptions.

Nebraska has developed a WBC threshold (binomial sampling plan) App available at Apple store.

Minnesota published an Environmental Entomology article on contamination and management of resistance evolution to high-dose transgenic insecticidal crops.

Iowa has developed a method using Coates molecular markers to return to a pure Z strain colony in Iowa. This is an improvement over current methods, as you can check different life stages and both males and females with this technique.

Nebraska is developing ECB first and second generation threshold decisions mobile apps.

Michigan and Wisconsin published a Handy Bt Trait Table which is utilized nationally.

A chapter R. Peshin, and D. Pimental (eds.), Integrated Pest Management  Experiences with Implementation, Vol. 4, Springer (in press), Emerging issues in Integrated Pest Management implementation and adoption in the North Central USA, emphasized to the lay public, regulators, and scientists throughout the world that the source of problems with developing insect and weed resistance to transgenic corn are not due to the new technologies themselves, but to inadequate integration into an IPM framework.

A High-throughput transcriptome sequencing for SNP and gene discovery in a moth (Environmental Entomology (2012) 41:997-1007) provides significant genetic resources for future research on western bean cutworm, and demonstrates the power of applying second-generation sequencing to previously under-studied species.

The review Population genetics strategies to characterize long-distance dispersal of insects Kyung Seok Kim and Thomas W. Sappington illustrates the kinds and range of problems impacted by insect dispersal that can be addressed through application of population genetics strategies.

A practical guide to Microsatellite data analysis for population genetics (Kyung Seok Kim and Thomas W. Sappington) was published that uses microsatellites in population genetics applications that will make it possible for both students and experienced scientists to analyze and apply their genotype data immediately to questions related to gene flow and population genetic structure. It provides links to freeware and details of pitfalls and other considerations in working with such data, that usually one must learn by trial-and-error.

A trapping network for Western bean cutworm was established (WBC, http://thebugspot.org/WBC, Fig. 2A) in collaboration with Dr. Andy Michel from Ohio State University. In 2012 150 unique sites in Michigan were established. Volunteers entered trap counts at the web site www.msuent.com. At the end of each week, we mapped the trap catch, and made the data available on the web site and through the Fast Fonz Facts email newsletter.

A keynote address to the 4th World Congress on Rural Life (Papal Consul on Justice and Peace) was presented at the Vatican using data from this project.

Many additional invited presentations were made all on different topics using data from this project. For example, six were given at the 56th Annual Meeting of the Japanese Society of Applied Entomology and Zoology, Tomio, Nara, Japan; University of Minnesota, St Paul, Minnesota; Embrapa, Cenargen, Brasilia, DF, Brazil; and the International Conference on Modern Biotechnologies: Sustainable Innovation and Regulatory Needs, Penang, Malaysia.

Hands-on trainings on corn insect pests were conducted at the 2012 Eastern Crop Scout School including use of pheromone traps (Iowa and New York lures) for monitoring for European corn borer populations in non-Bt field corn in north central and southwest North Dakota in 2012.

A series of videos was developed on corn and soybean IPM in collaboration with the UW--Madison Integrated Pest and Crop Management program (IPCM). Available at: http://ipcm.wisc.edu/video/

Jiang, X., H. Zhai, L. Wang, L. Luo, T. W. Sappington, and L. Zhang. 2012. Cloning of the heat shock protein 90 and 70 genes from the beet armyworm, Spodoptera exigua, and expression characteristics in relation to thermal stress and development. Cell Stress and Chaperones 17: 67-80.

Hellmich, R. L. & Hellmich, K. A. (2012) Use and Impact of Bt Maize. Nature Education Knowledge 3(10):4.

Miller, Nicholas J., Jing Sun, Thomas W Sappington. 2012. High-throughput transcriptome sequencing for SNP and gene discovery in a moth. Environmental Entomology 41:997-1007.

Xie, Daosong, Lizhi Luo, Thomas W. Sappington, Xingfu Jiang, Lei Zhang. 2012. Comparison of Reproductive and Flight Capacity of Loxostege sticticalis (Lepidoptera: Pyralidae), Developing From Diapause and Non-Diapause Larvae. Environmental Entomology 41:1199-1207.

Romeis, J., et al. Deriving criteria to select arthropod species for laboratory tests to assess the ecological risks from cultivating arthropod-resistant genetically engineered crops. Chemosphere, http://dx.doi.org/10.1016/j.chemosphere.2012.09.035.

Siegfried, B.D. and R.L. Hellmich. 2012. Understanding successful resistance management: The European corn borer and Bt corn in the United States. Special Issue of GM Crops and Foods 3: 184-193.

Paula-Moraes, S. V., T. E. Hunt, R. J. Wright, G. L. Hein, and E. E. Blankenship. 2012. On-Plant Movement and Feeding of Western Bean Cutworm (Lepidoptera: Noctuidae) Early Instars on Corn. Environ. Entomol. 41(6): 1494-1500.

Lindroth, E., T. E. Hunt, S. R. Skoda, M. D. Culy, D. Lee, and J. E. Foster. 2012. Population Genetics of the Western Bean Cutworm (Lepidoptera: Noctuidae) Across the United States. Ann. Entomol. Soc. Am. 105(5): 685-692.

Hein, G. L., K.L. Steffey, W.D. Hutchison, M.E. Rice, C.D. Pilcher, T.E. Hunt. 2012. Reply to Greenpeace Letter to the Editor Concerning Hutchison et al. (2011). J. Integ. Pest Mngmt. 3(3): 1-2.

Yao, J., L. L. Buschman, B. Oppert, C. Khajuria, K. Y. Zhu. 2012. Characterization of cDNAs encoding serine proteases and their transcriptional responses to Cry1Ab protoxin in the gut of Ostrinia nubilalis larvae. PLoS ONE 7(8): e44090. doi:10.1371/journal.pone.0044090

David S. Wangila, D.S., B.R. Leonard, Y. Bai, G.P. Head, and F. Huang. 2012. Larval survival and plant injury of Cry1Ab-susceptible, -resistant, and -heterozygous genotypes of the sugarcane borer on transgenic corn containing single or pyramided Bt genes. Crop Protect. 42:108-115.

Yang, Y., Y.-C. Zhu, Y-C., J. Ottea1, C. Husseneder, B. R. Leonard, C. Abel, R, Luttrell, and F. Huang. 2012. Characterization and mRNA quantitative analyses of cDNAs encoding three trypsin- and chymotrypsin-like proteinases in Cry1Ab-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Insect Science. DOI 10.1111/j.1744-7917.2012.01514.x

Huang, F., M.N. Ghimire, B.R. Leonard, C.D. Daves, R. Levy, J. Baldwin. 2012. Extended monitoring of resistance to Bacillus thuringiensis Cry1Ab maize in Diatraea saccharalis (Lepidoptera: Crambidae). GM Crop. Food: Biotech. Agri. Food Chain. 3:245-254.

Huang, F., M.N. Ghimire, B. R. Leonard, Y-C. Zhu, and G. Head.2012. Susceptibility of field populations of sugarcane borer from non-Bt and Bt maize plants to five individual Cry toxins. Ins. Sci. 19:570-578.

Bai, Y., R.Yan, G. Ye, F. Huang, D.S. Wangila, J. Wang, and J. Cheng. 2012. Field response of aboveground non-target arthropod community to transgenic Bt-Cry1Ab rice plant residues in postharvest seasons. Transgenic Res. 21:10231032.

Guo, Z., Y.C. Zhu, F. Huang, R. Luttrell, and R. Leonard. 2012. Microarray analysis of global gene regulation in the Cry1Ab-resistance and - susceptible strains of Diatraea saccharalis. Pest Manag Sci 2012; 68: 718730.

Wang, C, G. Henderson, F. Huang, B. Gautam, and C. Zhu. 2012. Survival rate, food consumption, and tunneling of the Formosan subterranean termite (Isoptera: Rhinotermitidae) feeding on Bt and non-Bt maize. Sociobiol. 59:1335-1350.

Hu, Y. and D. A. Andow. 2011. Field observations of Ostrinia nubilalis eclosion and posteclosionn activity of females around their natal plants. Insect Science 18: 712-718. DOI 10.1111/j.1744-7917.2011.01408.x

Glaum, P. R., A. R. Ives and D. A. Andow. 2012. Contamination and management of resistance evolution to high-dose transgenic insecticidal crops. Theoretical Ecology 5: 195-209. DOI 10.1007/s12080-010-0109-6

Andow, D. A. and G. L. Lövei. 2012. Cry toxins in transgenic plants have direct effects on natural enemies in the laboratory. Environmental Entomology 41(5): 1045-47. DOI: http://dx.doi.org/10.1603/EN11238

Burkness, EC. & W.D. Hutchison. 2012. Bt Pollen Dispersal and Bt Kernel Mosaics: Integrity of Non-Bt Refugia for Lepidopteran resistance management in Maize. J. Econ. Entomol. 105(5): 1773-1780.

White, J.A., E.C. Burkness, and W.D. Hutchison. 2012. Biased sex ratios, mating frequency and Nosema prevalence in European corn borer, at low population densities. J. of Applied Entomol. doi: 10.1111/j.1439-0418.2012.01738.x (in press): http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291439-0418/earlyview

Bell, J.R., E.C. Burkness, A.E. Milne, D.W. Onstad, M. Abrahamson, K.L. Hamilton, and W.D. Hutchison. 2012. Putting the brakes on a cycle: bottom-up effects damp cycle amplitude. Ecology Letters 15: 310-318.

Kang, J., D.W. Onstad, R.L. Hellmich, S.E. Moser, W.D. Hutchison & J.R. Prasifka. 2012. European corn borer model for studying the effect of non-transgenic corn ears fertilized by pollen of corn expressing Cry toxin. Environ. Entomol. 41(1): 200-211.

Morey, A.C., W.D. Hutchison, R.C. Venette & E.C. Burkness. 2012. Cold hardiness of corn earworm (Lepidoptera: Noctuidae) pupae. Environ. Entomol. 41(1): 172-179.

Dutra, C.C., R.L. Koch, E.C. Burkness, M. Meissle, J. Romeis, W.D. Hutchison and M.G. Fernandes. 2012. Harmonia axyridis (Coleoptera: Coccinellidae) exhibits no preference between Bt and non-Bt maize fed Spodoptera frugiperda (Lepidoptera: Noctuidae). PLoS One. 7(9): e44867. doi:10.1371/journal.pone.0044867

Shelton, A. M., S. Naranjo, J. Romeis, and R. H. Hellmich. 2012. Errors in Logic and Statistics Plague a Meta-Analysis (response to Andow et al. 2012). Environ. Entomol. 41:1047-9. DOI: http://dx.doi.org/10.1603/EN11238

Tian, J., H. L. Collins, J. Romeis, S. E. Naranjo, R. L. Hellmich and A. M. Shelton. 2012. Using field-evolved resistance to Cry1F maize in a lepidopteran pest to demonstrate no adverse effects of Cry1F on one of its major predators. Transgenic Research. 21:1303-1310. DOI 10.1007/s11248-012-9604-4.

Paula-Moraes, Silvana V., Wayne J. Ohnesorg, Eric C. Burkness, Gary L. Hein, Thomas E. Hunt, Robert J. Wright and William D. Hutchison. 2012. Western Bean Cutworm Speed Scouting. Apple iPod Application, Apple, Inc.

Paula-Moraes, Silvana V., Wayne J. Ohnesorg, Eric C. Burkness, Gary L. Hein, Thomas E. Hunt, Robert J. Wright and William D. Hutchison. 2012. Western Bean Cutworm Speed Scouting Spreadsheet EC1585.

Dana, Logan A., Thomas E. Hunt. 2012. Control of Second Generation European Corn Borer, 2011. Arthropod Management Tests 2012, Vol. 37, Pg. F10.