Brief Summary of Minutes of Annual Meeting

The NCERA-200 2012 annual meeting was held on October 30, 2012 in Ames, Iowa, from 8:30 AM to 1PM. In order to provide the participants with a comprehensive overview of emerging soybean viruses and novel technologies of virus research, this years symposium featured leaders in the areas of virus-induced gene silencing, plant anti-viral resistance, high throughout sequencing-based virus discovery, as well as innovative approaches aimed at controlling insect pests of soybean. The theme of the symposium was: Emerging Diseases and Technologies. The featured speakers this year are, Les Domier of University of Illinois at Chaimpaign, Steve Whitham of Iowa State University, Aardra Kachroo of University of Kentucky, and Bryony Bonning of Iowa State University. The committee is especially grateful to Steve Whitham for the time and efforts he invested to make the symposium a great success. The committee further expressed the gratitude to the United Soybean Board for the financial support, which covered the cost of the room rental, as well as travel costs of one of the speakers. The business meeting took place shortly after the completion of the symposium, with participants from five different states (Illinois, Iowa, Ohio, Arkansas, and Tennessee). Steve Slack, the administrative advisor of NCERA 200 provided a brief assessment of the current status of the group and praised the effort of the group at maintaining the tradition of having an annual symposium component of the annual meeting. He then went on to remind the participants of the need of developing Impact Statements that include illustrative images. The group then went on to discuss the chronic problem of low attendance of annual meetings. It was concluded that the steady decline of the number of plant virologist in the region, as well as shortage of research support, could have contributed to the problem. The consensus reached at the conclusion of the meeting is that we should explore the possibility of coordinating our meeting with the soybean disease multi-state group. Representatives from the states then presented the research updates during the past year. The meeting adjourned at 1PM. The full set of minutes attached includes each state report which also reflects their accomplishments. Please reference the attached minutes for the committee's accomplishments.

See attached summary of minutes for the complete report that contains the accomplishments of each state within their individual state reports.

Zhang, X., Sato, S.. Ye, X., Dorrance, A. E., Morris, T. J., Clemente, T. E., and Qu, F. 2011. Robust RNAi-based resistance to mixed infection of three viruses in soybean plants expressing separate short hairpins from a single transgene. Phytopathology 101, 1264-1269. Han, J., Domier, L. L., Dorrance, A. E., and Qu, F. (2012). Complete genome sequence of a novel pararetrovirus isolated from soybean. Journal of Virology 86, 9555. Nam, M., Koh, S., Kim, S. U., Domier, L. L., Jeon, J. H., Kim, H. G., Lee, S. H., Bent, A. F., and Moon, J. S. 2011. Arabidopsis TTR1 causes LRR-dependent lethal systemic necrosis, rather than systemic acquired resistance, to Tobacco ringspot virus. Molec. Cells 32(5), 421-429. Han. J., Domier, L. L., Dorrance, A., and Qu, F. 2012.Complete genome sequence of a novel pararetrovirus isolated from soybean. J. Virol. 86(17), 9555. Hill, C. B., Chirumamilla, A. and Hartman, G. L. 2012. Resistance and virulence in the soybean-Aphis glycines interaction Euphytica 186: 635-646. Hobbs, H.A., Domier, L. L. and Nelson, B. D. 2012.First Report of Alfalfa mosaic virus and Soybean dwarf virus on Soybean in North Dakota. Plant Dis. doi:10.1094/PDIS-07-12-0673-PDN. Hobbs, H. A., Jossey, S., Wang, Y., Hartman, G. L., and Domier, L. L. 2012.Diverse soybean accessions identified with temperature-sensitive resistance to Tobacco streak virus. Crop Sci. 52(2), 738-744. Lim, H. S., Jang, C. Y., Nam, J., Li, M., Hong, J. S., Bae, H., Ju, H. J., Kim, H. G., Ford, R. E., and Domier, L. L. 2012. Characterization of the in vitro activities of the P1 and helper component proteases of Soybean mosaic virus strain G2 and Tobacco vein mottling virus. Plant Pathol. J. 28(2), 197-201. McClellan, M. S., Domier, L. L., and Bailey, R. C. 2012. Label-free virus detection using silicon photonic microring resonators. Biosen. Bioelec. 31(1), 388-392. Pandey, A. K., Yang, C., Zhang, C., Graham, M., Horstman, H. D., Lee, Y., Zabotina, O. A., Hill, J. H., Pedley, K. F., and Whitham, S. A. 2011. Functional analysis of the Asian soybean rust resistance pathway mediated by Rpp2. MPMI 24:194-206. Hajimorad, M. R., Wen, R.-H., Eggenberger, A. L., Hill, J. H., and Saghai Maroof, M. A. 2011. ExperimentalAdaptation of an RNA Virus Mimics Natural Evolution. J. Virol. 85:2557-2564. Pandey, A. K., Yang, C., Zhang, C., Graham, M. A., Horstman, H. D., Lee, Y., Zabotina, O. A., Hill, J. H., Pedley, K. F., and Whitham, S. A. 2011. Functional analysis of the Asian soybean rust resistance pathway mediated by Rpp2. Mol. Plant Microbe Interact. 24: 194206. Bradshaw, J. D., Zhang, C., Hill, J. H., and Rice, M. E. 2011. Landscape epidemiology of Bean pod mottle comovirus: Molecular evidence of heterogeneous sources. Arch Virol. 156:1615-1619. He, B., Hill, J. H., and Hajimorad, M. R. 2011. Factors to improve detection of Alfalfa mosaic virus in soybean. Online. Plant Health Progress doi: 10.1094/PHP-2010-0926-02-RS. Chowda-Reddy, R. V., Sun, H., Hill, J., Poysa, V., and Wang, A. 2011. Simultaneous mutations in multi-viral proteins are required for an avirulent Soybean mosaic virus isolate to gain virulence n Rsv1-, Rsv3-, and Rsv4-genotype soybeans. PloS ONE 6(11): e28342. doi:10.1371/journal.pone.00283 Haidi, B., Bradshaw, J., Rice, M., and Hill, J. 2012. Bean leaf beetle (Coleoptera:Chrysomelidae) and Bean pod mottle virus in soybean: Biology, ecology, and management. Journal of Integrated Pest Management. DOI: http://dx.doi.org/10.1603/IPM11007 Liu, J-Z., Horstman, H. D., Braun, E., Graham, M. A., Zhang, C., Navarre, D., Qiu, W-L., Lee, Y., Nettleton, D., Hill, J. H., and Whitham, S. A. 2011. Soybean homologs of MPK4 negatively regulate defense responses and positively regulate growth and development. Plant Physiology 157:1363-1378. Zhang, C., Grosic, S., Whitham, S. A., and Hill, J. H. 2012. The Requirement of Multiple Defense Genes in Soybean Rsv1Mediated Extreme Resistance to Soybean mosaic virus. Molecular Plant Microbe Interact. 25:1307-1313. Juvale, P. S., Hewezi, T., Zhang, C., Kandoth, P. K., Mitchum, M. G., Hill, J. H., Whitham, S. A., and Baum, T. J. 20112. Temporal and spatial Bean pod mottle virus-induced gene silencing in soybean. Journal of Molecular Plant Pathology. DOI: 10.1111/J.1364-3703.2012.00808.X Liu, S., Kandoth, P. K., Warren, S. D., Yeckel, G., Heinz, R., Alden, J., Yang, C., Aziz, J., El-Mellouki, T., Juvale, P. S., Hill, J., Baum, T. J., Cianzio, S., Whitham, S. A., Korkin, D., Mitchum, M., and Meksem, K. 2012. A soybean cyst nematode resistance gene points to a new mechanism of plant resistance to pathogens. Nature. DOI:10.1038/nature11651 Khatabi, B., Wen, R.-H., Hershman, D. E., Kennedy, B. S., Newman, M. A., and Hajimorad, M. R. 2012. Generation of polyclonal antibodies and serological analyses of nucleocapsid protein of Soybean vein necrosis-associated virus: A distinct soybean infecting tospovirus serotype. European Journal of Plant Pathology 133, 783-790. Khatabi, B., He, B., and Hajimorad, M. R. 2012. Diagnostic potential of polyclonal antibodies against bacterially expressed recombinant coat protein of Alfalfa mosaic virus in soybean. Plant Disease 96, 1352-1357. Khatabi, B., Fajolu, O. L., Wen, R.-H., and Hajimorad, M. R. 2012. Evaluation of North American isolates of Soybean mosaic virus for gain of virulence on Rsv-genotype soybeans with special emphasis on resistance-breaking determinants on Rsv4. Molecular Plant Pathology 13, 1077-1088.