SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

TAC Members: Florida (University of Florida) Kevin E. Kenwothy (kkenworthy@ufl.edu) Georgia (University of Georgia) Paul L. Raymer (praymer@griffin.uga.edu) Guam (University of Guam) Mari Marutani (marutani@uguam.uog.edu) Louisiana (Louisiana State University) Don LaBonte (dlabonte@agctr.lsu.edu) North Carolina (North Carolina State Univ)Tom Stalker (tom_stalker@ncsu.edu) South Carolina (Clemson University) Emerson R. Shipe (eshipe@clemson.edu) Tennessee (University of Tennessee) Fred Allen (allenf@utk.edu) Virgin Islands (University of the Virgin Islands) Thomas W. Zimmerman (tzimmer@uvi.edu) Southern Assoc. Agric. Gerald Arkin, Administrative Advisor Exp. Sta. Directors (garkin@uga.edu) Griffin PGRCU Staff: USDA-ARS-PGRCU, Griffin, GA Gary A. Pederson, Research Leader (gary.pederson@ars.usda.gov) Noelle Barkley (elle.barkley@ars.usda.gov) Melanie Harrison-Dunn (melanie.harrisondunn@ars.usda.gov) Roy N. Pittman (roy.pittman@ars.usda.gov) Robert Jarret (bob.jarret@ars.usda.gov) John B. Morris (brad.morris@ars.usda.gov) Ming Li Wang (mingli.wang@ars.usda.gov) Lee Ann Chalkley (leeann.chalkley@ars.usda.gov) Merrelyn Spinks (merrelyn.spinks@ars.usda.gov) Phiffie Vankus (phiffie.vankus@ars.usda.gov) Libbie Hudson (libbie.hudson@ars.usda.gov) Kami Lindberg (kami.lindberg@ars.usda.gov) Other Attendees: USDA-ARS, SAA, Athens, GA Deborah Brennan (deborah.brennan@ars.usda.gov)

The meeting was called to order by Paul Raymer (Chair) at 1:07 pm on August 2, 2011 on the University of Georgia, Griffin, GA campus at the University of Georgia Research and Education Garden building. Paul welcomed attendees and introductions were done around the room. Kevin Kenworthy, Secretary, took the minutes. Dr. Gerald Arkin, the administrative advisor for the S-009 RTAC and Associate Dean of the Griffin Campus, welcomed the group to the Griffin Campus. He was excited to have the group back on campus and stated that he remains committed to the program as it relates to the plant industry, the conservation of germplasm and making germplasm publically available. He stated that progress has been made in the Griffin program and that the quality of the preserved material has improved. In particular, he noted that the collection is in great shape despite the current uncertainties surrounding their budget. He shared some comments from the National Plant Germplasm Coordinating Committee which had raised the question, Should NPGS charge for requested material? It was deemed that this was impractical and that the germplasm system would be worse off if charges were imposed. He further commented that both the UGA Deans and the southern directors appreciate the program. Paul Raymer asked for a motion to approve the 2010 minutes which was made and seconded. The motion was approved. He also asked for any additions to the agenda. There was a request to have comments from the Units molecular geneticist and pathologist to relate their activities with use of the germplasm. An update and presentation on the webpage was also requested. These items were added to the end of the agenda. Fred Allen and Tom Zimmerman were appointed to nominate a secretary and location for next year. Gary Pederson provided a report on the National Program staff and noted some changes in personnel. He discussed website development and that a new version of GRIN is in the works. Gary gave a presentation that provided a summary of PGRCU for the year and the current status of collections, the size of collections, the number of available accessions and the number of backed up accessions. He noted that all numbers continue to increase and that germination testing has increased in the last year for sorghum, cowpea, peanuts, grasses and that 77% of the overall collection has been tested for germination. An update of storage capabilities was provided and he noted that current -18 C storage is full. The distribution of accessions went to 49 states (none to Alaska) and 47 other countries (and U.S. territories). A comment was made that U.S. territories should be included with the states. Gary states that these figures are provided by GRIN and that including territories with the states would require a change in GRIN. Gary concluded the PGRCU report with an update on funding, staff numbers (soon to advertise a peanut S-009 technician position), curator changes and building construction. A written regeneration plan was provided for all in attendance and each curator made a presentation on status of their collection, regeneration and characterization in 2011, and regeneration of the entire collection. Reports were given by Gary Pederson on the clover and sorghum collections, Brad Morris on the miscellaneous legumes collection, Bob Jarret on the vegetable crops collection, Roy Pittman on the Vigna collection, Melanie Harrison-Dunn on the warm season grass collection, and Noelle Barkley on the peanut collection. Ming Li Wang provided a verbal report on his activities investigating chemical characterization of germplasm with different curators. David Pinnow provided a verbal report on virology activities and noted the recent completion of a 20 year project to screen for viruses in the sweet potato collection. At 5:00pm the meeting was adjourned until 8:00am August 3rd. The meeting re-convened at 8:00am, August 3rd, at the University of Georgia Research and Education Garden building, Griffin, GA and departed for a tour of PGRCU facilities. We were able to see field facilities, a new equipment storage pole barn, many of the collections, tour the cold storage facilities and see the construction progress on a new 4 C storage unit that will enable a previous 4 C storage facility to be changed to -18 C storage. At 10:00am the committee returned to the University of Georgia Research and Education Garden building, Griffin, GA. State reports were presented by Tom Zimmerman (University of Virgin Islands), Emerson Shipe (South Carolina), Kevin Kenworthy (Florida), Fred Allen (Tennessee), Tom Stalker (North Carolina), Mari Marutani (Guam), Don LaBonte (Louisiana), and Paul Raymer (Georgia). Written reports were distributed for Alabama, Hawaii, Kentucky, Oklahoma, Texas, and Virginia. The 2012 S-009 meeting will be held in Griffin, GA. The dates of August 1-2 or 8-9, 2012 were discussed and the dates were tentatively set for July 31  August 1, 2012 starting at 1:00 pm on July 31st. The incoming S-009 Chair will be Kevin Kenworthy. Mari Muratani was nominated to be the incoming S-009 secretary. There were no other nominations and Mari was elected. Tom Stalker thanked the staff for presentations and for showing the plans for regeneration. He recommended that the group report back to their respective Deans and Directors to enforce the importance of the National Plant Germplasm System. Paul Raymer thanked everyone for their participation. Tom Stalker motioned to adjourn the meeting and Fred Allen seconded the motion. The meeting was adjourned at 11:25am.

Accomplishments

The complete accomplishments section for the 2011 S-009 annual report is located at the S-009 website (www.ars.usda.gov/Main/docs.htm?docid=9514). A summary of the accomplishments from the Griffin location and S-009 cooperators is listed below. USDA, ARS, Plant Genetic Resources Conservation Unit: A total of 90,942 accessions of 1,534 plant species were maintained in the Griffin plant genetic resources collection. Over 87.7% of accessions were available for distribution to users and over 97.1% were backed up for security at a second location. Bulk seed samples for 66,995 accessions were maintained at -18 C for long-term storage with seed of the remaining accessions stored at 4 C. A total of 28,308 seed and clonal accessions in 925 separate orders were distributed upon request to scientists and educators in 49 U.S. states and 47 foreign countries. Acquisitions made to the collection included 143 sorghum, 144 warm-season grasses, 19 vegetable, and 2 other accessions. Seed regenerations and characterization were conducted on 299 peanut, 131 cowpea, 46 warm-season grass, 60 pepper, 361 legume, new, and misc. crop, 91 annual clover, and 30 other vegetable accessions. A plant collection trip was conducted in Alabama, Florida, Georgia, and South Carolina for naturalized Sorghum halepense germplasm that is in demand for sorghum gene flow studies. Seed regenerations and characterization were conducted on 299 peanut, 131 cowpea, 46 warm-season grass, 60 pepper, 361 legume, new, and misc. crop, 91 annual clover, and 30 other vegetable accessions. Over 300 pepper accessions were grown in the field for regeneration, characterization and recording of digital images. Digital images of cowpea, sorghum, cucurbit, and watermelon accessions, and pepper capsaicin content, peanut core selection, sorghum, and sweet sorghum data were added to the Germplasm Resources Information Network (GRIN). Long-term maintenance of 241 wild peanut and 458 warm-season grass accessions was continued in the greenhouse. Over 30 warm-season grass accessions and 712 sweetpotato accessions were maintained in tissue culture with eight replications of each sweetpotato clone. Germination testing has been completed for 69,556 accessions (over 77% of collection) since 2002. In cooperation with vegetable industry plant pathologists, differential sets of four vegetable crops are being established and distributed to researchers for identification of disease races. Fatty acid content was determined for the entire U.S. castor bean (over 1,000 accessions) and sesame (over 1,200 accessions) collections and 98 okra accessions. Over 660 castor accessions were genotyped with 15 SSR markers for further genetic classification. A population of over 1,900 mutant watermelon seeds was developed for a mutant TILLING study with ARS cooperators in Charleston, SC. Ploidy level was determined for the entire St. Augustine and seashore paspalum grass collections. Photoperiod-sensitive Neonotonia, Teramnus, and annual clover accessions were successfully regenerated in the greenhouse. A total of 92 peanut accessions were successfully evaluated under quarantine and disease-free seed was produced. In association with Kansas State University scientists, 1,000 biomass sorghum accessions will be evaluated for plant morphology, biochemical composition, and genotype. Alabama: Current work focuses on evaluation, utilization and breeding of sunn hemp and sericea lespedeza species. Sunn hemp germplasm is being used for development of cultivars for the continental US. Two sunn hemp cultivars were released. Sericea lespedeza is being evaluated for control of gastrointestinal parasites in ruminants and for condensed tannin content. A new low-growing sericea lespedeza will be evaluated for road-side use with colleagues at Auburn, GA, LA, AR, and USDA at several locations. Current work focuses on evaluation, utilization and breeding of upland cotton. Cotton accessions continue to be evaluated to resistance heat and drought. We are also conducting a study on the impact of exotic germplasm introgression on cotton yield and fiber quality traits. We are evaluating 300 soybean accessions per year in a USDA-sponsored evaluation of germplasm for resistance to Asian soybean rust. Florida: Florida was very active in 2010 for plant genetic resources distribution and use, with 31 different individuals requesting materials from 18 different genera of plants and a total of 1,230 unique PIs distributed. Affiliation of individuals obtaining materials included University of Florida scientists, USDA scientists, private research organizations, private citizens, and public schools. Most individuals who responded to a request for information indicated a high level of satisfaction with materials provided and appreciation for the availability of the germplasm. Research and educational training was conducted with bahiagrass, Hemarthria, gamagrass, wiregrass, perennial peanut, lettuce, pepper, clover, sunn hemp, peanut, sweet and grain sorghum, and bermudagrass accessions. Georgia: During 2010, 31 separate requests for plant germplasm were made to the S-9 unit by the citizens of Georgia. As a result of these requests, 332 plant accessions were supplied to University scientists, USDA scientists, consultants, seed companies, gardeners, and individuals. The most requested crops were peanut, warm-season grasses, sorghum, and watermelon. The Institute of Plant Breeding, Genetics, and Genomics (IPBGG) has 22 faculty members, 10 MS students, and 13 Ph.D. students. Georgia plant breeding programs supply new crop cultivars and associated technologies to our agricultural sector and rely heavily upon the plant materials maintained within the S-9 unit. UGA currently has active cultivar development programs in soybean, peanut, small grains, turf grass, forages, blueberry, pecan, grape and numerous ornamental crops that frequently utilize the plant genetic resource collections. The entire seashore paspalum collection was screened for salt tolerance in 2010. Accessions of the warm-season grass collection are being evaluated for new mutations that would be useful in developing herbicide resistance in turfgrass. The S-009 Unit remains a critical component of our research and cultivar development programs in Georgia. Guam: Conservation efforts continued for local germlines of sweetpotato (Ipomoea batatas), chili pepper (Capsicum annuum) and field corn (Zea mays). Evaluation of selected cultivars of eggplant (Solanum melongena) and cucumber (Cucumis sativus) was conducted as on-farm trials. Sunn hemp (Crotalaria juncea) including 15 obtained from USDA/ARS/PGRCU Griffin GA, and a local check (a Taiwan variety commonly used in Guam) were tested for their field performance in calcareous Guam cobbly clay soil (pH 7.8) from 7/16/10 to 9/27/10. The majority reached the reproductive stage at 72 days after planting except three accessions, PI 468956, PI 561720, and PI 234771. Early maturation was observed with four accessions of PI 207657, PI 391567, PI 250487, and PI 322377. Tropic Sun developed in Hawaii was a late maturing cultivar that produced the greatest biomass at harvest. Conservation and evaluation of important tropical plant germlines will support development of sustainable agriculture in the region. Searching for new germlines and commercial cultivars with heat tolerance and pest resistance will assist growers to choose locally adapted vegetables and green manure plants to sustain their manageable farming operation in Guam. Hawaii: The USDA/ARS, Hilo Germplasm Unit continued to manage 14 tropical fruit and nut crops at the clonal repository in Hilo Hawaii (approx. 1000 accessions). The collection represents a diverse economically important collection of tropical fruit and nut crops such as pineapple, macadamia, guava, papaya, pili nut, peach palm, breadfruit, lychee and longan, carambola and their relatives. Preliminary tests were initiated on tissue culture storage of rambutan, pulasan and Canarium germplasm. Germplasm introductions were 32 and 34 distributions were made of 240 items. Evaluation activities included: 1) guava germplasm tolerance to the Oriental fruit fly using cage inoculation of fruits at different maturities. 2) Kapoho solo seeds regenerated at our PRSV free field in Paauilo were planted to select for and maintain a uniform source of the Kapoho papaya germplasm. 3) Observations of growth and production of ohelo at three locations continued. Three clonal selections for berry and ornamental potted plants production were released and tissue culture plugs of two ohelo selections were made available. Continued working with the S.W. Forestry University in Yunnan and two representatives from Hainan China to plan for future plant germplasm exchanges. Louisiana: Hibiscus sabdariffa germplasm seeds were screened to select early maturing hibiscus accessions for Louisiana climatic conditions. Out of 17 accessions, one failed to germinate. One accession from South Africa showed promise. Seed of Desmanthus illinoensis received from the NPGS will be combined with other native materials and used for variety development. Malvaceae family species are being screened for odd-chain unsaturated fatty acids in their seed oils. These fatty acids were identified in the initial accessions and additional accessions will be screened. Research on Ipomoea accessions include evolutionary ecology, molecular evolution and population genetics of genes involved in flower color, as well as the phylogenetic systematics of morning glories (species of the tribe Ipomoeae). Rice and red rice lines were used to investigate the genetics of photoperiodic flowering response. North Carolina: Faculty in the Crop Science and Horticultural Science Departments at NC State University conduct research on strawberry, blueberry, brambles, tree crops, ornamentals, maize, soybean, peanut, cotton, tobacco, small grains, turfgrasses, sweetpotato, cucurbits, and other crops. Priorities are on incorporating disease and insect resistance, abiotic stress resistance, and quality factors into improved breeding lines and cultivars. Plant introductions are critical components of plant improvement programs, and NCSU scientists make use of germplasm maintained in the National Plant Germplasm System (NPGS). During the past year, 18 individuals received 115 entries to NC from the Griffin station, including peanut, sorghum, sweetpotato, pepper, watermelon and warm season grasses. The U.S. collection of cultivated and wild Nicotiana species is maintained at NCSU and numerous seed requests are filled annually both to U.S. and international individuals and organizations. A duplicate collection of Arachis species is being developed with 405 of the 607 accession in the Griffin collection maintained at NCSU. During the past year, NC Agricultural Research Service released 1 peanut cultivar, 1 soybean germplasm, two soybean cultivars, 6 maize lines, 1 tomato breeding line, two tomato cultivars, 5 mountain landscape cultivars, two butterfly bush cultivars, 3 redbud cultivars, 1 cucumber cultivar, and two cucumber inbreds. Oklahoma: Plant germplasm distribution data received from S-9 indicate that 1,576 plant accessions maintained at the Plant Genetic Resources Conservation Unit at Griffin, GA were distributed to organizations or individuals in Oklahoma the last year, from August, 2010 through July, 2011. The number of distributed plants last year is the highest in recent years, if not a record. The accessions were distributed in 67 respective requests. Respective annual plant germplasm distribution numbers for 2006, 2007, 2008, and 2009 were 169, 295, 431 and 352. The requested plant species in 2010 included sorghum (Sorghum bicolor) (1,104 accessions), peanuts (136), Panicum (113), Guar (Cyamopsis sp.) (33), peppers (21), watermelon (20), okra (4), squash (Cucurbita spp.) (6), Vigna spp. (8), and Miscanthus and other warm-season grasses (10), and other legumes (13). Users of the plant accessions included researchers of Oklahoma State University, the Noble Foundation, USDA-ARS laboratories, and individual Oklahomans. Puerto Rico: Eighteen quenepa (Melicoccus bijugatus) cultivars are in the sixth year of evaluation at Juana Díaz and Lajas. Horticultural traits and fruit quality parameters (pH, TSS, acid, and TSS/acid ratio) were measured on the mandarin cultivars Encore, Murcott and Fallglo on five rootstocks in Corozal. At Isabela, a citrus germplasm collection was established in the screenhouse and citrus rootstocks are being propagated for new experiments to be established at Corozal and Adjuntas. Five hybrid and five open-pollinated Cubanelle-type pepper cultivars were evaluated from February to May. Twelve Mayaguezano-type mango clones on Banilejo dwarfing rootstock are being evaluated in the field at Lajas. A field planting of 14 guava accessions is being evaluated at Juana Díaz. Marketable yield of Estela hybrid tannier was increased by 27% when planting material from main corm sections was used, compared with crowns. An increase in average bunch weight of 18% and a reduction of 32 days from planting to fruiting in Cuerno de Arce plantain was observed with the addition of chicken manure. One hundred half-sib lines of Suresweet sweet corn are being evaluated in a replicated performance trial. Germplasm requests in Puerto Rico in 2010 include 5 Crotalaria sp. and 4 Mucuna sp. New crop germplasm projects in 2011 include evaluation of accessions of upland rice, breadfruit and achachairu (Garcinia sp.), screening of citrus rootstocks for Phytophthora resistance, and evaluation of traditional varieties produced by the Agricultural Experiment Station. South Carolina: A total of 481 germplasm accessions were distributed by PGRCU, Griffin, GA to the following individuals in South Carolina in 2010: Dr. Amnon Levi, USDA Vegetable Laboratory, Charleston, SC, 49 Citrullus, 14 Praecitrullus (watermelon), 9 gourd, and 1 Cucurbita accessions; Dr. H. Knap, Clemson University, 1 Pueraria (legume) accession; Dr. Ellis Caniglia, USDA Vegetable Laboratory, Charleston, SC, 2 Citrullus accessions; Dr. S. Kresovich, University of South Carolina, 383 Sorghum accessions; Dr. J. Bohac,, 20 Ipomoea (sweetpotato) accessions: K. Hazel, 1 Vigna (cowpea) accession; and Dr. M. Shepherd, Clemson University, 1 Vigna accession. In 2010, twenty elite breeding lines and/or cultivars from the Clemson University soybean breeding program were tested in a greenhouse to determine their suitability as hosts for reniform nematode, Rotylenchulus reniformis, and identify resistant genotypes. Based on nematode resistance traits and performance in non-nematode infested field nurseries, SC07-786 and SC07-1490 will be further evaluated in 2011 Southern Regional Soybean Tests for potential cultivar release. A total of 64 soybean plant introductions have been identified that show varying levels of resistance to soybean rust disease caused by the fungus Phakopsora pachyrhizi. The plant introductions confirmed to be resistant in these evaluations should be useful sources of genes for resistance to North American populations of P. pachyrhizi. Tennessee: A number of projects are being conducted at the University of Tennessee in which plant introductions are being utilized in research. Three PIs are being intercrossed with two experimental lines and two released cultivars for the purpose of developing new synthetic varieties of switchgrass. Early generation lines from the GEM project, expired PVP lines, and other germplasm obtained from Ames PI station maize collection were crossed with elite adapted lines. Progeny from crosses were advanced by traditional breeding methods, to develop new maize parental lines. Recombinant inbred lines and near-isogenic lines of soybean were developed from a cross between a prolific rooting line, PI 416937, and a high leaflet orienting cultivar, USG 5601T. A total of 18 soybean genotypes including 15 high oleic PIs and three checks were planted 16 different environments across five locations during three years and fatty acid composition was determined. The experimental soybean line TN03-349 is an F6-derived line from the cross TN93-99 x PI 416937 and was released as the new edible vegetable soybean cultivar, NUTRIVEG Soy6407. Heterogeneous plant populations developed from exotic Chinese plant introductions by Dr. Randy Nelson, have been grown at the East Tennessee Research and Education Center and locally adapted single-plant selections are under development and field testing. Texas: Daryl Morishige (TAMU) utilized the Sorghum bicolor BTx623*IS3620c Recombinant Inbred Population for QTL analysis to identify the genes controlling flowering time underlying the QTL. MMR Genetics and USDA-ARS have a collaborative project (Re-Initiated Sorghum Conversion) funded by the National Sorghum Checkoff to identify genomic regions associated with yield potential, and convert these materials to shorter heights and early maturity (removal of photoperiod sensitivity). Accessions stored at Griffin, GA from an Ethiopian Collection, Sudan Collection and Mali Collection were requested for this project. Bob Klein (USDA) has a research program to characterize the sorghum germplasm for allelic variation in a key flowering time gene. Gary Peterson (TAMU, Lubbock) is evaluating grain sorghum response to salt stress in selected germplasm. Tom Juenger, UT Austin, requested and received seed for two GRIN accessions of Panicum hallii (PI 229052 and PI 229051) to use genetic mapping and gene expression studies to understand the genetic basis of abiotic stress responses, including drought tolerance, in these grasses. Russell Jessup (TAMU) ordered accessions of Urochloa brizantha for comparison of candidate apomixis genes between Urochloa and Pennisetum spp. Guy Hallman (USDA, Weslaco, TX) requested bell pepper (Capsicum annuum) germplasm to evaluate summer fruit set in extreme south Texas. Mark Burow (TAMU) requested peanut germplasm as botanical type specimens for evaluation of the US peanut minicore collection, for parents to use for crossing to incorporate leafspot resistance, and as parents to use for crossing to incorporate early maturity and cold tolerance. Virgin Islands: Virus-free sweet potato plantlets, obtained from the Griffin location are being grown in culture to supply clean plantlets to local growers. Sweet potatoes can be successfully maintained for a year on low sucrose medium to reduce growth and revitalized on fresh medium with 3% sucrose to regain active growth for micropropagation. Viruses in sweet potatoes are a problem that reduces growth and production over time. Most of the 41 sweet potato leaf samples on the island of St. Croix were positive for at least one virus. Fifty percent of the originally clean UVI material had virus after 120 days. There are multiple viruses that can potentially affect papaya varieties. Three viruses, Papaya Ring Spot Virus (PRSV), Papaya Mosaic Virus (PMV), and Cauliflower Mosaic Virus (CaMV) were tested for. The results indicated that 100% contained PRSV, 30% CaMV and 18% PMV. Work at UVI-AES has been on going to develop papaya varieties that tolerate the virus and still be productive. Sorrel (Hibiscus sabdariffa), is popular during the winter holiday season in the Caribbean for its colorful fleshy calyces. Planting sorrel in September at 16 inches was shown to increase production per length of row. Sorrel is sensitive to high pH calcareous soils found in areas of St Croix. Varieties from Africa were found to have better tolerance to the high pH soil, ranked above 2.5, than the Caribbean varieties which were rated below 2. From these results, a breeding plan will be developed to combine tolerance to calcareous soils with a day neutral photoperiod to enhance year round production. Sorrel was found to have similar nutraceutical bioflavonoid compounds as found in cranberries and blueberries. Plant breeding has begun to increase the bioflavonoid content and develop a day-neutral caliche tolerant variety for year round production. Virginia: Germplasm from S-009 was acquired in Virginia by researchers at both Virginia Tech and Virginia State University. A joint project between Gregory Welbaum and Bingyu Zhou in the Department of Horticulture at Virginia Tech has been focused on screening Citrullus, Lageraria, and Preacitrullus accessions for resistance to the watermelon pathogen that causes fruit blotch, with the eventual objective of releasing blotch resistant cultivars. Bingyu Zhao has also been studying host plant resistance in Panicum accessions to different bacterial pathogens. Sheena Friend, Virginia Tech, has conducted a phylogenetic study on the genus Arachis using germplasm acquired from S-009. Several accessions of three legume crops, Guar (Cluster bean  Cyamposis tetragonoloba), Sunn hemp (Crotalaria) and Lablab were evaluated by Harbans Bhardwaj at Virginia State for potential production in Virginia. In another study, Francoise Favi has been growing Cympogon accessions in the greenhouse to test volatiles and plants extracts on whitefly (Bemisia tabacci) and post-harvest insect pests. Maria Virginia Sanchez Puerta, at the Universidad Nacional de Cuyo in Argentina, has used accessions of solanaceous species to test for resistance to root-knot nematode (Meloidogyne spp.). These activities document the distribution and utilization of plant genetic resources, a primary objective of the regional project, in Virginia.

Impacts

  1. The germplasm collection at Griffin, Georgia has increased to 90,942 accessions of 255 genera and 1,534 species. Currently, 97.1% of the collection is backed up at the National Center for Genetic Resource Preservation, Ft. Collins, CO, and 87.7% of the accessions are available for use by the research community. Backing up safely secures these plant genetic resources for future use by researchers and good availability provides users with a wide array of currently available germplasm.
  2. In 2010, a total of 28,308 seed, tissue culture, and clonal accessions were distributed to users for their research use. All accessions were requested from the Griffin location directly by researchers and distributed in 924 orders to users in 49 states and 47 foreign countries, with 13,477 accessions distributed to users in the S-009 states. Genetic resources maintained at the Griffin location provide a valuable resource for crop improvement research.
  3. Seed quality is evaluated by germination testing and seed longevity is improved through -18 C storage. Germination tests were conducted on over 12,500 accessions in the last year with over 77% of the entire collection at Griffin tested for germination since 2002. The program continued to split seed samples of all accessions at Griffin with a small distribution sample maintained at 5 C and the bulk of each sample maintained at -18 C to maximize seed longevity. Currently, almost 75% (almost 67,000 accessions) of the entire Griffin collection has at least one sample in -18 C storage.
  4. Characterization and evaluation of the genetic resources enables researchers to more efficiently utilize the Griffin germplasm collection. Digital images of cowpea, sorghum, chile pepper and warm-season grass accessions were taken. Variation for seed oil content was determined for the entire watermelon (>1,000 accessions) collection. Fatty acid composition was determined for the entire sesame and castor bean collections. Ploidy level and salt tolerance were determined for the entire seashore paspalum collection. Virus screening was completed on the entire sweetpotato collection for sweetpotato leaf curl virus. Characterization for descriptors was conducted on chile pepper, warm-season grasses, cowpea, peanut, misc. legume, annual clover, and other accessions. All evaluation, characterization, and image data was uploaded onto the GRIN database.
  5. Additional impacts and future plans are noted within the complete 2010 S-010 annual report located at the S-009 website (www.ars.usda.gov/Main/docs.htm?docid=9514).

Publications

Plant Genetic Resources Conservation Unit Barkley, N.L., Wang, M.L., Pittman, R.N. 2011. Can high quality DNA be extracted and utilized from Arachis seeds in long term storage with zero percent germination? American Peanut Research and Education Society Abstracts. San Antonio, TX 7/15/2011. Barkley, N.L., Chamberlin, K.D., Wang, M.L., Pittman, R.N. 2011. Genotyping and fatty acid composition analysis in segregating peanut (Arachis hypogaea L.) populations. Peanut Science 38:11-19. Barkley, N.L., Wang, M.L., Pittman, R.N. 2011. A real-time PCR genotyping assay to detect FAD2A SNPs in peanuts (Arachis hypogaea L.). Electronic Journal of Biotechnology 14(1). Available: http://www.ejbiotechnology.info/index.php/ejbiotechnology/issue/view/65. Burow, G.B., Klein, R.R., Klein, P.E., Franks, C.D., Burke, J.J., Xin, Z., Pederson, G.A., Schertz, K.F. 2011. Registration of the IS3620C/BTx623 recombinant inbred mapping population of sorghum (Sorghum bicolor L. [Moench.]). Journal of Plant Registrations 5(1):141-145. Gremillion, S.K., Culbreath, A.K., Gorbet, D.W., Mullinix Jr, B.G., Pittman, R.N., Stevenson, K.L., Todd, J.W., Escobar, R.E., Condori, M.M. 2011. Field evaluations of leaf spot resistance and yield in peanut genotypes in the United States and Bolivia. Plant Disease 95(3):263-268. Gremillion, S., Culbreath, A., Gorbet, D., Mullinix, B., Pittman, R.N., Stevenson, K., Todd, J., Condori, M. 2011. Response of progeny bred from Bolivian and North American cultivars in integrated management systems for leaf spot of peanut (Arachis hypogaea). Crop Protection Journal 30(6):698-704 Guo, B. Wang, M.L. 2010. Genetic Enhancement of Peanut Oil Quality and Disease Resistance. United States Japan Natural Resources Forage Seed Panel. Pages 49-51. Guo, B., Qin, H., Feng, S., Chen, C.Y., Knapp, S., Culbreath, A., He, G., Wang, M.L., Zhang, X., Holbrook Jr, C.C. 2011. An Integrated Linkage Map for Cultivated Peanut Derived from Two RILs Populations. Plant and Animal Genome Conference. San Diego January 15-19, 2011. Harrison Dunn, M.L., Pederson, G.A. 2010. Expansion of the USDA Switchgrass Germplasm Collection. ASA-CSSA-SSSA Annual Meeting Abstracts. Harrison Dunn, M.L., Hotchkiss, M.W. 2010. Bamboo: An Underutilized Resource with Extensive Application Possibilities. American Bamboo Society Annual Conference. Harrison Dunn, M.L. 2010. Curation of the U.S. Collection of Warm Season Grass Germplasm. International Symposium on Forage, Turfgrass and Biofuel Germplasm Research. Oct. 2010. Holbrook Jr, C.C., Burrow, M.D., Isleib, T.G., Pittman, R.N. 2010. Status of the core and the mini core collections for the U.S. gemrplasm collection of peanut. Proceeding of the American Peanut Research and Education Society 42:80. Jarret, R.L., Wang, M.L., Levy, I.J. 2011. Seed oil and Fatty acid content in okra (Abelmoschus esculentus) and related species. Journal of Agricultural and Food Chemistry 59(8):4019-4024. Morris, J.B., Chase, C., Cho, A., Koenig, R., Morales-Payan, J. 2010. Principal component analysis for morphological, seed reproductive, and phenology traits in 16 sunn hemp (Crotalaria juncea L.) accessions. American Society of Horticulture Science Meeting 45(8):S234 (Abstr.) Morris, J.B., Grusak, M.A., Wang, M.L. 2010. Mineral, Fatty Acid, Flavonoid, and Isoflavonoid variability in Lablab purpureus L. accessions. ASA-CSSA-SSSA Annual Meeting Abstracts. 89:1. Morris, J.B., Chase, C.A., Cho, A.H., Koenig, R.L., Morales-Payan, J.P. 2010. Earliness, morphological, and reproductive variation among 16 sunn hemp (Crotalaria juncea L.) accessions in Griffin, GA. Association for the Advancement of Industrial Crops Conference. Ft. Collins, CO. Sept. 18-22, 2010. Morris, J.B., Wang, M.L., Morse, S.A. 2011. Ricinus. Wild Crop Relatives: Genomic and Breeding Resources. 3:251-260. Morris, J.B., Wang, M.L. 2011. Evaluation for morphological, reproductive, anthocyanin index, and flavonol traits in ornamental and nutraceutical producing Hibiscus species. Ornamental Plants: Types, Cultivation and Nutrition. Hauppauge, NY: Nova Publishers. p.111-127. Morris, J.B. 2011. Morphological, Phenological, and Reproductive Trait Analysis for the Pasture Species, Siratro [Macroptilium atropurpureum (DC.) Urb.]. Tropical Grasslands. 44:266-273. Morris, J.B., Wang, M.L. 2011. Anthocyanin indexes, quercetin, kaempferol, and myricetin concentration in leaves and fruit of Abutilon theophrasti Medik. genetic resources. Plant Genetic Resources: Characterization and Utilization 1:1-3. Mosjidis, J.A., Wang, M.L. 2011. Crotalaria. Wild Crop Relatives: Genomic and Breeding Resources, Industrial Crops. 63-69 DOI: 10.1007/978-3-642-21102-7_3. Pederson, G.A. 2010. National Plant Germplasm System: Critical Role of Customer Service. ASA-CSSA-SSSA Annual Meeting Abstracts. Oct. 2010. Pederson, G.A. 2010. Status of the U.S. Sweet Sorghum Collection for Biofuel Research. International Symposium on Forage, Turfgrass and Biofuel Germplasm Research. Oct. 2010. Qin, H., Li, Y., Guo, Y., He, G., Chen, C.Y., Culbreath, A., Knapp, S.J., Cook, D.R., Holbrook Jr, C.C., Wang, M.L., Guo, B. 2010. Identification of molecular markers associated with resistance to TSWV through genetic mapping [abstract]. Presented at the American Phytopathological Society Annual Meeting in Nashville, TN. on August 7-11, 2010. Wang, M.L., Barkley, N.L., Chinnan, M., Stalker, T., Pittman, R.N. 2010. Oil content and fatty acid composition variability in wild peanut species. Plant Genetic Resources. 8:232-234. Wang, M.L., Sukumaran, S., Barkley, N.L., Chen, Z., Chen, C.Y., Guo, B., Pittman, R.N., Stalker, H., Holbrook Jr, C.C., Pederson, G.A., Yu, J. 2011. Population Structure and Marker-Trait Association Analysis of the U.S.Peanut (Arachis hypogaea L.) Mini-Core Collection. Journal of Theoretical and Applied Genetics. DOI: 10.1007/s00122-011-1668-7. Wang, M.L., Barkley, N.L., Chen, Z., Pittman, R.N. 2011. FAD2 Gene Mutations Significantly Alter Fatty Acid Profiles in Cultivated Peanuts (Arachis hypogaea). Biochemical Genetics. DOI: 10.1007/s10528-011-9447-3. Wang, M.L., Sukumaran, S., Barkley, N.L., Chen, Z., Chen, C.Y., Guo, B., Pittman, R.N., Pederson, G.A., Yu, J. 2011. Genetic diversity and population structure analysis of accessions in the U.S. peanut mini-core collection. Plant and Animal Genome Conference. p.138. Wang, M.L., Morris, J.B., Pinnow, D.L., Davis, J., Raymer, P., Pederson, G.A. 2010. A survey of the castor oil content, seed weight and seed-coat colour on the United States Department of Agriculture germplasm collection. Plant Genetic Resources: Characterization and Utilization. 8:229-231. Wang, M.L., Morris, J.B., Tonnis, B.D., Pinnow, D.L., Davis, J., Raymer, P., Pederson, G.A. 2011. Screening the Entire USDA Castor Germplasm Collection for Oil Content and Fatty Acid Composition for Optimum Biodiesel Production. Journal of Agricultural and Food Chemistry. DOI:10.102/jf202949v. Wang, M.L., Chen, C.Y., Davis, J., Stalker, T., Pittman, R.N., Holbrook Jr, C.C., Pederson, G.A. 2010. Assessment of oil content and fatty acid composition variability in the U.S. peanut minicore. ASA-CSSA-SSSA Annual Meeting Abstracts. 295:5. Xin, Z., Wang, M.L. 2011. Sorghum as a Versatile Feedstock for Bioenergy Production. Biofuels. 2(5) 577-588. Alabama Burke J.M., N.C. Whitley, D.A. Pollard, J.E. Miller, T.H. Terrill, K.E. Moulton, J.A. Mosjidis. 2011. Dose titration of sericea lespedeza leaf meal on Haemonchus contortus infection in lambs and kids. Veterinary Parasitology. In Press Mosjidis, J.A. 2011. AU Red Ace, a red clover for the Lower South. Journal of Plant Registrations 5:1-3. doi: 10.3198/jpr2010.01.0020crc; Published online 10 Nov. 2010. Mosjidis, Jorge A. and Glenn Wehtje. 2011. Weed control in Sunn Hemp (Crotalaria juncea L.) and its ability to suppress weed growth. Crop Protection 30:70-73. doi:10.1016/j.cropro.2010.08.021 Burke J.M., S. Orlik, J.E. Miller, T.H. Terrill, J.A. Mosjidis. 2010. Using copper oxide wire particles or sericea lespedeza to prevent a peri-parturient gastrointestinal nematode infection in sheep and goats. Livestock Science 132:13-18. Mosjidis, J.A. 2010. Sericea lespedeza biomass composition for bioenergy in the Southeastern USA. Field and Vegetable Crops Research 47:1-10. Chapala, M. M, D. B. Weaver, B. T. Campbell, E. van Santen, and R. R. Sharpe. 2011. Exotic germplasm introgression effects on adapted cotton genotypes. p. 699 - 704 In S. Boyd, M. Huffman, B. Robertson (ed.). Proc. Beltwide Cotton Conf. Atlanta, GA. Sikkens, R. B., S. Moore, K. Lawrence, T. Wu and D. B. Weaver. 2011. LONREN Germplasm Response to Nematode Inoculation Level. p. 728 - 736 In S. Boyd, M. Huffman, B. Robertson (ed.). Proc. Beltwide Cotton Conf. Atlanta, GA. Sürmelioglu, Çigdem, R. B. Sikkens, R. R. Sharpe, S. R. Moore, E. van Santen, K. S. Lawrence, and D. B. Weaver. 2010. Resistance to reniform nematode from G. hirsutum and G. longicalyx sources: a comparison. p. 787 - 788 In S. Boyd, M. Huffman, B. Robertson (ed.). Proc.Beltwide Cotton Conf. New Orleans, LA. Weaver, D. B., R. B. Sikkens, S. Moore, K. Lawrence and R. Sharpe. 2011. LONREN × FM966 Progeny Evaluation In a Field Infested with Reniform Nematode. p. 737 - 741 In S. Boyd, M. Huffman, B. Robertson (ed.). Proc. Beltwide Cotton Conf. Atlanta, GA. Walker, D. R., H. R. Boerma, D. V. Phillips, R. W. Schneider, J. B. Buckley, E. R. Shipe, J. D. Mueller, D. B. Weaver, E. J. Sikora, S. H. Moore, G. L. Hartman, M. R. Miles, D. K. Harris, D. L. Wright, J. J. Marois, and R. L. Nelson. 2011. Evaluation of USDA soybean germplasm accessions for resistance to soybean rust in the southern United States. Crop Sci. 51:(in press). Florida Acuna, Carlos, T.R. Sinclair, C.L. Mackowiak, A.R. Blount, K.H. Quesenberry, and W.W. Hanna. 2010. Potential root depth development and nitrogen uptake by tetraploid bahiagrass hybrids. Plant and Soil 334:491-499. Acuna, C.A., A.R. Blount, K.H. Quesenberry, K.E. Kenworthy, and W.W. Hanna. 2010. Tetraploid bahiagrass hybrids: breeding technique, genetic variability and proportion of heterotic hybrids. Euphytica. DOI 10.1007/s10681-010-0276-y Aina, Olubunmi, Kenneth Quesenberry, and Maria Gallo. 2010. In Vitro Induction of Autotetraploids In Wild Peanut (Arachis paraguariensis). In ASA-CSSA-SSSA-GCA Abstracts 2010 [CD-ROM]. ASA, CSSA, and SSSA, Madison, WI. Carvalho, Marcelo Ayres, Kenneth H. Quesenberry, and Maria Gallo. 2010. Comparative assessment of variation in the USA Arachis pintoi (Krap. And Greg.) germplasm collection using RAPD profiling and tissue culture regeneration ability. Plant Syst. Evol. 288:245-251. Gorbet, D.W and Barry L. Tillman. 2011. Registration of York peanut. Journal of Plant Registrations. 5:1-6. Kenworthy, K.E., A.R. Blount, W.T. Crow, P.F. Harmon, E.A. Buss, and K.H. Quesenberry. 2010. Germplasm evaluation of alternate warm-season turfgrass species for use in Florida and lower latitudes. p. 191-194. In Proceeding of 2010 International Symposium on Forage, Turf-grass, and Biofuel Germplasm Research, Yengling, China. Oct 10-12 2010. Kenworthy, K.E., A.R. Blount, F. Altpeter, K.H. Quesenberry. 2010. Breeding value added bahiagrass. Fla. Turf Dig.27(5):p. 12-20. Maas, Andrea L., William F. Anderson, and Kenneth H. Quesenberry. 2010. Genetic Variability of Cultivated Rhizoma Peanut. Crop Sci. 50:1908-1914. Milla-Lewis, S., M. C. Zuleta, G. Van Esbroeck, K. H. Quesenberry, and K. E. Kenworthy. 2010. Molecular and cytological assessment of genetic diversity in stenotaphrum germplasm. . In ASA-CSSA-SSSA-GCA Abstracts 2010 [CD-ROM]. ASA, CSSA, and SSSA, Madison, WI. Prine, G.M., E.C. French, A.R. Blount, M.J. Williams, and K.H. Quesenberry. 2010. Registration of Arblick and Ecoturf rhizoma peanut germplasms for ornamental or forage use. J. Plant Registrations. 4:145-148. Quesenberry, K.H., A.R. Blount, P. Mislevy, E.C. French, M.J. Williams and G.M. Prine. 2010 Registration of UF Tito and UF Peace rhizoma peanut cultivars with high dry matter yields, persistence, and disease tolerance. J. Plant Registrations 4:17-21. Quesenberry, Kenneth H., Judith M. Dampier, Y. Y. Lee, Rex L. Smith, and Carlos A. Acuña. 2010. Doubling the Chromosome Number of Bahiagrass via Tissue Culture. Euphytica 175:43-50. Quesenberry, Kenneth. 2010. Importance of a Consensus Research Agenda for the Genetic Improvement of Perennial (and Annual) Legumes: Science and Policy. Proceedings of the NAAIC and Trifolium Conference. 27-30 July, 2010 Boise, ID. Published online at www.naaic.org . Quesenberry, Kenneth H., Judith Dampier, and Ann R. Blount. 2010. Barduro: A mid-dormant red clover with root-knot nematode resistance. Proceedings of the NAAIC and Trifolium Conference. 27-30 July, 2010 Boise, ID. Published online at www.naaic.org . Sandhu, S., A.R. Blount, K.H. Quesenberry, and F. Altpeter. 2010. Apomixis and ploidy barrier suppress pollen-mediated gene flow in field grown transgenic turf and forage grass (Paspalum notatum Flüggé). Theoretical and Applied Genetics (published on-line May 30, 2010). Sandhu, S., A.R. Blount, K. Quesenberry, and F. Altpeter. 2010. Apomixis and ploidy barrier suppress pollen-mediated gene flow in field grown transgenic turf and forage grass (Paspalum notatum Flügge´). Theor Appl Genet. 121:919929. Schwartz B., K. Kenworthy, M. Engelke, A. Genovesi, R. Odom, and K. Quesenberry. 2010. Variation in 2C nuclear DNA content of Zoysia spp. as determined by flow cytometry. Crop Sci. 50:1519-1525. Schwartz, B., K. Kenworthy, W. Crow, J. Ferrell, G. Miller, and K. Quesenberry. 2010. Variable responses of zoysiagrass genotypes to the sting nematode. Crop Sci. 50: 723-729 Wang, Z., Y.Q. Wu, K.E. Kenworthy. 2010. Genetic diversity of common carpetgrass revealed by amplified fragment length polymorphism markers. Crop Sci. 50:1366-1374. Louisiana LaBonte, D.R., C.A. Clark, T.P. Smith, and A.Q.Villordon. 2011. 'Bonita' Sweetpotato. HortScience 46:1-2 Mcharo, M. and D. LaBonte. 2010. Multivariate selection of AFLP markers associated with b-carotene in sweetpotatoes. Euphytica 175:123132. North Carolina Balota, M., T.G. Isleib, J.W. Chapin. 2010. Description and performance of Virginia market-type peanut cultivars. Va. Coop. Ext. Serv. Publ. 432-201 (on line at pubs.ext.vt.edu/432/432-201/432-201.html). Branch, W.D., M. Balota, T.G. Isleib, J.W. Chapin, J.P. Bostick, B.L. Tillman, M.D. Burow, M.R. Baring, and K.D. Chamberlin. 2010. Uniform Peanut Performance Tests, 2009. Univ. Georgia Coastal Plain Exp. Stn. Prog. Rep. No. 4-10. 24 p. Buckler, E.S., J.B. Holland, P.J. Bradbury, C.B. Acharya, P.J. Brown, C. Browne,E. Ersoz, S. Flint-Garcia, A. Garcia, J.C. Glaubitz, M.M. Goodman, C. Harjes, K. Guill, D.E. Kroon, S. Larsson, N.K. Lepak, H. Li, S.E. Mitchell, G. Pressoir, J.A. Peiffer, M.O. Rosas, T.R. Rocheford, M.C. Romay, S. Romero, S. Salvo, H.S. Villeda, H.S. da Silva, Q. Sun, F. Tian, N. Upadyayula, D. Ware, H. Yates, J. Yu, Z. Zhang, S. Kresovich, and M.D. McMullen. 2009. The genetic architecture of maize flowering time. Science 325: 714-718. Call, A. D. and T. C. Wehner. 2010. Search for higher resistance to the new race of downy mildew in cucumber. Proc. Cucurbitaceae 2010, Charleston, SC, p. 112-115 (eds. J. A. Thies, S. Kousik, A. Levi). Cardinal A.J., J.W. Burton, A. Camacho-Roger, R. Whetten, A.S. Chappell, K.D. Bilyeu, J.Auclair, R.E.Dewey. Molecular analysis of GmFAD3A in two soybean populations segregating for the fan, fap1, and fapnc loci. Crop Science.(in press). Cervantes-Flores, J. C., B. Sosinski, K.V. Pecota, R.O.M. Mwanga, G. L. Catignani, V. D. Truong, R.H. Watkins, M. R. Ulmer and G.C. Yencho. 2010. Identification of quantitative trait loci for dry-matter, starch, and b-carotene content in sweetpotato. Mol Breeding. DOI 10.1007/s11032-010-9474-5. Clough, M., G. Yencho, B. Christ, W. DeJong, D. Halseth, K. Haynes, M. Henninger, C. Hutchinson, M. Kleinhenz, G. Porter, and R. Veilleux. 2010. An interactive online database for potato varieties evaluated in the eastern US. HortTechnology 20:245-249. Costa, J.M., H.E. Bockelman, G. Brown-Guedira, S.E. Cambron, X. Chen, A. Cooper, C. Cowger, Y. Dong, A. Grybauskas, Y. Jin, J. Kolmer, J.P. Murphy, C. Sneller, and E. Souza. 2010. Registration of the soft red winter wheat germplasm MD01W233-06-1 resistant to Fusarium head blight. J. Plant Reg. 4:255-260. Dell, E.A., D. Bowman, T. Rufty, and W. Shi. 2010. The community composition of soil-denitrifying bacteria from a turfgrass environment. Research in Microbiology 161:315-325. Dewey, R.E. and Zhang, P. 2010. Candidate gene analysis of mutant soybean germplasm. In K. D. Bilyeu, M.B. Ratnaparkhe, C. Kole, eds., Genetics, Genomics and Breeding of Soybean. CRC Press, Boca Raton, FL. pp. 187-197. Dia, M. and T. C. Wehner. 2010. Efficient breeding methods for yield in watermelon: a review. Proc. Cucurbitaceae 2010, Charleston, SC, p. 19-21 (eds. J. A. Thies, S. Kousik, A. Levi). Eller, M.S., G.A. Payne, and J.B. Holland. 2010. Selection for reduced Fusarium ear rot and fumonisin content in advanced backcross maize lines and their topcross hybrids. Crop Sci 50:2249-2260. Friend, S.A., D. Quandt, S.P. Tallury, H.T. Stalker, and K.W. Hilu. 2010. Species, genomes and section relationships in genus Arachis (Fabaceae): A molecular phylogeny. Plant Systematics and Evolution 290:185-199. Gardner, R.G. and Panthee, D.R. 2010. Grape tomato breeding lines: NC 1 Grape, NC 2 Grape and NC 3 Grape. HortScience, 45: 1887-1888. Gardner, R.G. and Panthee, D.R. 2010. NC 1 CELBR and NC 2 CELBR: Early blight and late blight resistant fresh market tomato breeding lines. HortScience, 45: 975-976. Gardner, R.G. and Panthee, D.R. 2010. 'Plum Regal' fresh-market plum tomato hybrid and its parents, NC 25P and NC 30P. HortScience, 45: 824-825. Gilsinger, J.J., J.W. Burton, T.E. Carter, Jr. 2010. Maternal effects on fatty acidgenes mediate nornicotine biosynthesis in Nicotiana tabacum L.: functional characterization of the CYP82E10 gene. Phytochem. 71:1988-1998. Gilsinger, J.J., J.W. Burton, T.E. Carter, Jr. 2010. Maternal effects on fatty acid composition of soybean seed oil. Crop Sci. 2010 50:1874-1881. Glover, J.D., J.P. Reganold, L.W. Bell, J. Borevitz, E.C. Brummer, E.S. Buckler, C.M. Cox, T.S. Cox, T.E. Crews, S.W. Culman, L.R. DeHaan, D. Eriksson, B.S. Gill, J. Holland, F. Hu, B.S. Hulke, A.M.H. Ibrahim, W. Jackson, S.S. Jones, S.C. Murray, A.H. Paterson, E. Ploschuk, E.J. Sacks, S. Snapp, D. Tao, D.L. Van Tassel, L.J. Wade, D.L. Wyse, Y. Xu. 2010. Perennial questions of hydrology and climate - Response. Science 330:33. Glover, J.D., J.P. Reganold, L.W. Bell, J. Borevitz, E.C. Brummer, E.S. Buckler, C.M. Cox, T.S. Cox, T.E. Crews, S.W. Culman, L.R. DeHaan, D. Eriksson, B.S. Gill, J. Holland, F. Hu, B.S. Hulke, A.M.H. Ibrahim, W. Jackson, S.S. Jones, S.C. Murray, A.H. Paterson, E. Ploschuk, E.J. Sacks, S. Snapp, D. Tao, D.L. Van Tassel, L.J. Wade, D.L. Wyse, Y. Xu. 2010. Increasing food and ecosystem security through perennial grains. Science 328:1638-1639. Gonzalo, M., T.J. Vyn, J.B. Holland, and L.M. McIntyre. 2010. Direct mapping of density response in recombinant inbred lines of maize (Zea mays L.). Heredity 104:583599. Hebert, C.J., D.H. Touchell, T.G. Ranney, and A.V. LeBude. 2010. In vitro shoot regeneration and polyploidy induction of Rhododendron Fragrantissimum Improved. HortScience 45(4):801-804. Holbrook, C.C., M.D. Burow, T.G. Isleib and R.N. Pittman. 2010. Status of the Core and the Mini-Core Collections for the U.S. germplasm collection of peanut. Proc. Proc. Am. Peanut Res. Educ. Soc. 42: 80. (On-line at www.apresinc.com/Proceedings.html) Holland, J.B., and P.T. Nelson. 2010. Dedication: Major M. Goodman, maize geneticist and breeder. Plant Breeding Reviews 33:1-29. Hollowell, J.E., B.B. Shew, T.G. Isleib, and S.P. Tallury. 2010. Greenhouse evaluations of virginia-type breeding lines for resistance to Sclerotium rolfsii. Proc. Amer. Peanut Res. Educ. Soc. 41: 82. (On-line at www.apresinc.com/Proceedings.html). Howard, A., J.L. Heitman and D. Bowman. 2010. A simple approach for demonstrating soil water retention and field capacity. J. Nat. Res. & Life Sci. Ed. 39:120-124. Isleib, T.G., H.E. Pattee, T.H. Sanders, L.L. Dean, and K.W. Hendrix. 2010. Flavor profiles and composition of runner- and virginia-type cultivars tested as part of the Uniform Peanut Performance Test. Proc. Amer. Peanut Res. Educ. Soc. 41: 60-61. (On-line at www.apresinc.com/Proceedings.html). Isleib, T.G., S.C. Copeland, S.R. Milla-Lewis and M. Balota. 2010. Genetic gain for pod yield in the North Carolina State University peanut breeding project. Proc. Proc. Am. Peanut Res. Educ. Soc. 42: 79-80. (On-line at www.apresinc.com/Proceedings.html) Isleib, T.G., S.R. Milla-Lewis, H.E. Pattee, S.C. Copeland, M.C. Zuleta, B.B. Shew, J.E. Hollowell, T.H. Sanders, L.O. Dean, K.W. Hendrix, M. Balota, and J.W. Chapin. 2011. Registration of Bailey peanut. J. Plant Reg. 5: 27-39. [doi:10.3198/jpr2009.12.0742crc] Isreal, D.W., Earl Taliercio, P. Kwanyuen, J.W. Burton and Lisa Dean. 2011. Inositol metabolism in developing seed of low and normal phytic acid soybean lines. 2011. 51: 282-289. Jones, J.R., A.V. LeBude, and T.G. Ranney. 2010. Vegetative propagation of Oconee azalea (Rhododendron flammeum) by stem cuttings and mound layering. J. Environ. Hort. 28(2):69-73. Joshi, B.K. and Panthee, D.R. 2010. Information theory and multivariate techniques for analyzing DNA sequence data: An example from tomato genes. Nepal Journal of Biotechnology, 1: 1-8. Klosinska, U., E. U. Kozik, A. Call and T. C. Wehner. 2010. New sources of resistance to downy mildew in cucumber. Proc. Cucurbitaceae 2010, Charleston, SC, p. 135-138 (eds. J. A. Thies, S. Kousik, A. Levi). Kornegay, J. 2010. Toward Sustainable Agricultural Systems in the 21st Century. NRC (National Research Council), National Academies Press, Washington, DC, 570 pp. Kozik, E. U., U. Klosinska and T. C. Wehner. 2010. Progress in the development of cucumber breeding lines with low-temperature resistance. Proc. Cucurbitaceae 2010, Charleston, SC, p. 46-49 (eds. J. A. Thies, S. Kousik, A. Levi). Kumar, R. and T. C. Wehner. 2011. Discovery of second gene for solid dark green versus light green rind pattern in watermelon. J. Heredity 102: 489-493. Kuraparthy V., S. Sood, G. Brown-Guedira and B.S. Gill. 2010. Development of a PCR assay and marker-assisted transfer of leaf rust resistance gene Lr58 into adapted winter wheats. Euphytica (in press). Lewis, R.S. and R.E. Dewey. 2010. Genetics-based modification of tobacco chemistry in a regulatory environment. Recent Adv. Tob. Sci. 36: 5-24. Lewis, R.S., and C. Rose. 2010. Agronomic performance of TMV-resistant tobacco lines and hybrids possessing the resistance gene N introgressed on different chromosomes. Crop Sci. 50:1339-1347. Lewis, R.S., and C. Rose. 2011. Identification of Nicotiana tabacum haploids based on transgenic over-expression of PAP1 from Arabidopsis thaliana. Crop Sci. (In Press). Lewis, R.S., S.W. Bowen, M.R. Keogh, and R.E. Dewey. 2010. Three nicotine demethylase genes mediate nornicotine accumulation in tobacco: functional characterization of the CYP82E10 gene. Phytochemistry 71:1988-1998. Li, R. and R. Qu. 2011. High throughput Agrobacterium-mediated switchgrass transformation. Biomass and Bioenergy 35: 1046-1054. Li, R., A.H. Bruneau, and R. Qu. 2010. Morphological mutants of St. Augustine grass induced by gamma ray irradiation. Plant Breed. 129: 412-416 Li, R., A.H. Bruneau, and R. Qu. 2010. Morphological mutants of St. Augustinegrass induced by gamma ray irradiation. Plant Breed. 129: 412-416. Li, R., A.H. Bruneau, and R. Qu. 2010. Tissue culture-induced morphological somaclonal variation in St. Augustine grass [Stenotaphrum secundatum (Walt.) Kuntze] Plant Breed. 129: 96-99. Li, R., R. Qu, A.H. Bruneau, and D.P. Livingston. 2010. Selection for freezing tolerance in St. Augustinegrass through somaclonal variation and germplasm evaluation. Plant Breed. 129: 417-421. Maxwell, J.J., J.H. Lyerly, G. Srnic, R. Parks, C. Cowger, D. Marshall, G. Brown-Guedira and J.P. Murphy. 2010. MlAB10: A Triticum turgidum subsp. dicoccoides derived powdery mildew resistance gene identified in common wheat. Crop Sci. 50:2261-2267. McCord, P.H., B.R. Sosinski, K.G. Haynes, M.E. Clough, and G.C. Yencho. 2011. QTL mapping of internal heat necrosis (IHN) in tetraploid potato. Theor Appl Genet 122:129142. McCord, P.H., B.R. Sosinski, K.G. Haynes, M.E. Clough, and G.C. Yencho. 2011. Linkage mapping and QTL analysis of agronomic traits in tetraploid potato (Solanum tuberosum L. subsp. tuberosum). Crop Science. (in press). McCreight, J. D., T. C. Wehner and A. R. Davis. 2010. Turkmenistan melon (Cucumis melo), and watermelon (Citrullus lanatus) germplasm expedition 2008. Proc. Cucurbitaceae 2010, Charleston, SC, p. 139-142 (eds. J. A. Thies, S. Kousik, A. Levi). McMullen, M.D., S. Kresovich, H.S. Villeda, P. Bradbury, H. Li, Q Sun, S. Flint-Garcia, J. Thornsberry, C.B. Acharya, C. Bottoms, P. Brown, C. Browne, M. Eller, K. Guill, C. Harjes, D. Kroon, N, Lepak, S.E. Mitchell, B. Peterson, G. Pressoir, S. Romero, M.O. Rosas, S. Salvo, H. Yates, M. Hanson, E. Jones, S. Smith, J.C. Glaubitz, M. Goodman, D. Ware, J.B. Holland, and E.S. Buckler. 2009. Genetic properties of the maize nested association mapping population. Science 325: 737-740. Milla-Lewis, S.R., M.C. Zuleta and T.G. Isleib. 2010. Assessment of genetic diversity among U.S. runner-type peanut cultivars using simple sequence repeat markers. Crop Sci. 50: 2396-2405. [doi:10.2135/cropsci2010.04.0223] Milla-Lewis, S.R., M.C. Zuleta, and T.G. Isleib. 2010. SSR allelic diversity in virginia-type peanut cultivars released from 1943 to 2006. Crop Sci. 50: 1348-1356. [doi:10.2135/cropsci2009.09.0501] Milla-Lewis, S.R., M.C. Zuleta, and T.G. Isleib. 2010. Assessment of Genetic Diversity in U.S. Runner-type Peanut Cultivars Using Simple Sequence Repeat (SSR) Markers. Crop Sci. 50(6): Milla-Lewis, S.R., M.C. Zuleta, and T.G. Isleib. 2010. SSR allelic Diversity in Virginia-Type peanut cultivars released from 1943 to 2006. Crop Sci. 50(4):1348-1356. Miranda, L. A., D.E. Bland, S.E. Cambron, J.H. Lyerly, J. Johnson, G.D. Buntin, and J.P. Murphy. 2010. Genetic mapping of an Aegilops tauschii-derived Hessian fly resistance gene in common wheat. Crop Sci.50: 612-616. Murphy, J.P., and R. Navarro. 2010. The 2009-10 Southern Uniform Winter Wheat Scab Nursery. In: S. Canty, A.Clark, A. Anderson-Scully, D. Ellis, D. Van Sanford (eds.) Proceedings of the National Fusarium Head Blight Forum. 2010 Dec 7-9. Milwaukee, WI. Lexington, KY: University of Kentucky. p. 154-158. Nagy, E.D., Y. Chu, Y. Guo, S.K.S. Tang, Y. Li, W.B. Dong, P. Timper, C. Taylor, P. Ozias-Akins, C.C. Holbrook, V. Beilinson, N.C. Nielsen, H.T. Stalker, and S.J. Knapp. 2010. Recombination is Suppressed in an Alien Introgression on Chromosome 5A of Peanut Harboring Rma, a Dominant Root-Knot Nematode Resistance Gene. Molecular Breeding 26:357-370. Panthee, D.R. and Chen F. 2010. Genomics of fungal disease resistance in tomato. Current Genomics, 11:30-39. Panthee, D.R. and Gardner, R.G. 2010. 'Mountain Merit': A late blight resistant large-fruited hybrid tomato. HortScience, 45:1547-1548. Panthee, D.R. and Gardner, R.G. 2011. 'Mountain Majesty': A tomato spotted wilt virus resistant fresh-market hybrid tomato and its parents NC 714 and NC 1CS. HortScience, 46: (in press). Panthee, D.R. and Gardner, R.G. 2010. 'Mountain Merit': A late blight resistant large-fruited hybrid tomato. HortScience, 45: 1547-1548. Parris, J.K., T.G. Ranney, H.T. Knap, and W.V. Baird. 2010. Ploidy levels, relative genome sizes, and base pair composition in magnolia. J. Amer. Soc. Hort. Sci. 135(6):533-547. (JASHS cover). Perkins-Veazie, P. G. Ma and T. C. Wehner. 2010. Comparison of methods for chlorophyll estimation in cucumber peel. Proc. Cucurbitaceae 2010, Charleston, SC, p. 84-86 (eds. J. A. Thies, S. Kousik, A. Levi). Place, G.T., S.C. Reberg-Horton, D.A. Dickey and T.E. Carter, Jr. 2011. Identifying Soybean Traits of Interest for Weed Competition. Crop Sci (accepted). Place, G.T., S.C. Reberg-Horton, T.E. Carter, A.N. Smith. 2011. Effects of Soybean Seed Size on Weed Competition. Agronomy Journal. 103: 175-181. Place, G.T., S.C. Reberg-Horton, T.E. Carter, S.R. Brinton, A.N. Smith. 2010. Screening Tactics for Identifying Competitive Soybean Genotypes. Communications in Soil Science and Plant Analysis (accepted  in press). Reganold, J.P., D. Jackson-Smith, S.S. Batie, R.R. Harwood, J.L. Kornegay, D. Bucks, C.B. Flora, J.C. Hanson, W.A. Jury, D. Meyer, A. Schumacher, Jr., H. Sehmsdorf, C. Shennan, L.A. Thrupp, and P. Willis. 2011. Transforming U.S. agriculture. Science 332:670-671. Robbins, M.D., Masud, M.A.T., Panthee, D.R., Gardner, R.G., Francis, D.M. and Stevens, M.R. (2010). Marker assisted selection for coupling phase resistance to Tomato spotted wilt virus and late blight in tomato. HortScience, 45:1424-1428. Rounsaville, T.J. and T.G. Ranney. 2010. Ploidy levels and genome sizes of Berberis L. and Mahonia Nutt. species, hybrids, and cultivars. Hortscience 45:1029-1033. Ruiz-Rojas, J., D. Sargent, V. Shulaev, J. Pattison, S. Holt, A. Cordia and R. Veilleux. 2010. SNP discovery and genetic mapping of T-DNA insertional mutants. Theor. and Appl. Genet. 121:449-63. Sharma-Shivappa, R., A. Panneerselvam, P. Kolar, T. Ranney, and S. Peretti. 2010. Effect of ozonolysis on bioconversion of miscanthus to bioethanol. Annu. Intl. Mtg. Amer. Soc. Agr. Biol. Eng. (in press). Shi, W., D. Bowman and T. Rufty. 2011. Microbial Control of Soil Carbon Accumulation in Turfgrass Systems. In: Carbon Dynamics in Urban Ecosystems, R. Lal (ed.). Ohio State Univ. Press. (In press). Shrivastava, G., Rogers, M., Wszelaki, A., Panthee, D.R. and Chen, F. 2010. Plant volatiles-based insect pest management in organic farming. Critical Reviews in Plant Sciences, 29: 123-133. T.E. Carter, Jr., P.E. Rzewnicki, J.W. Burton, M.R. Villagarcia, D.T. Bowman, E. Taliercio, and P. Kwanyuen. 2010. Registration of N6202 Soybean Germplasm with High Protein, Favorable Yield Potential, Large Seed, and Diverse Pedigree. J. of Plant Registrations 4:77-79. Tetteh, A.Y., T.C. Wehner and A.R. Davis. 2010. Identifying resistance to powdery mildew race 2W in the USDA-ARS watermelon germplasm collection. Crop Sci. 50: 933-939. Trueblood, C.E., T.G. Ranney, N.P. Lynch, J.C. Neal, and R.T. Olsen. 2010. Evaluating fertility of triploid clones of Hypericum androsaemum L. for use as non-invasive landscape plants. Hortscience 45(7):1026-1028. Truong, Van-Den, N. Deighton, R.T. Thompson, R.F. McFeeters, L.O. Dean, K.V. Pecota, and G.C. Yencho. 2010. Characterization of anthocyanins and anthocyanidins in purple-fleshed sweetpotatoes by HPLC-DAD/ESI-MS/MS. J. Agric. Food Chem. 58: 404-410. van Heerwaarden, J., J. Doebley, W.H. Briggs, J.C. Glaubitz, M.M. Goodman, J.J. Sanchez G, and J. Ross-Ibarra. 2011. Genetic signals of origin, spread, and introgression in a large sample of maize landraces. PNAS 108:1088-1092. Vigouroux, Y., J.C. Glaubitz, Y. Matsuoka, M.M. Goodman, J. Sanchez, and J. Doebley. 2008. Population structure and genetic diversity of New World maize races assessed by DNA microsatellites. American Journal of Botany 95: 1240-1253. Vontimitta, V, and R.S. Lewis. 2011. Mapping of quantitative trait loci affecting resistance to Phytophthora nicotianae in tobacco (Nicotiana tabacum L.) line Beinhart-1000. Mol. Breed. (In Press). Vontimitta, V., D.A. Danehower, T. Steede, and R.S. Lewis. 2010. Analysis of a Nicotiana tabacum genomic region controlling two leaf surface chemistry traits. J. Agric. Food Chem. 58:294-300. Wang, M.L., N.A. Barkley, M. Chinnan, H.T. Stalker, and R.N. Pittman. 2010. Oil content and fatty acid composition variability in wild peanut species. Plant Genetic Resources: Characterization and Utilization. 8:232-234. Werner, D.J. and L.K. Snelling. 2010. 'Merlot' and 'Ruby Falls' redbud. HortScience 45: 146-147. Wherley, B., D. Bowman and T. Rufty. 2011. Effect of Soil Saturation on Development and 15N-Nitrate Uptake Efficiency of Two Warm Season Grasses Emerging from Dormancy. Comm. Soil Sci. Plant Anal. (In press). Zhang, S., H. Miao, X. Gu, Y. Yang, B. Xie, X. Wang, S. Huang, Y. Du, R. Sun and T.C. Wehner. 2010. Genetic mapping of the scab resistance gene in cucumber. J. Amer. Soc. Hort. Sci. 135: 53-58. Zwonitzer, J., D. Bubeck, D. Bhattramakki, M.Goodman, C. Arellano, and P. Balint-Kurti.2009. Use of backcross recurrent selection and QTL mapping to identify loci contributing to southern leaf blight resistance in a highly resistant maize line. Theoretical and Applied Genetics 118:911-25 Zwonitzer, J.C., N.D. Coles, M.D. Krakowsky, C. Arellano, J.B. Holland, M.D. McMullen, R.C. Pratt, and P.J. Balint-Kurti. 2010. Mapping resistance quantitative trait loci for three foliar diseases in a maize recombinant inbred line populationEvidence for multiple disease resistance? Phytopathology 100:72-79. Balota, M. and T. Isleib. 2010. Evaluation of Virginia-type peanuts for gas exchange and transpiration ratio. Proc. Proc. Am. Peanut Res. Educ. Soc. 42: 65-66. (On-line at www.apresinc.com/Proceedings.html). Balota, M., W.A. Payne, T.G. Isleib, and R.N. Pittman. 2010. Peanut variability for cold tolerance and water-use efficiency. Proc. Amer. Peanut Res. Educ. Soc. 41: 44. (On-line at www.apresinc.com/Proceedings.html). Benson, J., G. Brown-Guedira, J. Holland, J.P. Murphy, and C.H. Sneller. 2010. Association analysis of markers for breeding scab resistance in winter wheat. In: S. Canty, A. Clark, A. Anderson-Scully, D. Ellis, D. Van Sanford (Eds.). Proceedings of the National Fusarium Head Blight Forum. 2010 Dec 7-9. Milwaukee, WI. Lexington, KY: University of Kentucky. p. 128. Calbrix, R., H.T. Stalker and N. Nielsen. 2010. Systematic identification of 2S, 7S and 11S seed storage proteins of cultivated peanut. Proc. Amer. Peanut Res. Educ. Soc. 42:83-84. Cardinal, A.J. and S. Wang. North Carolina State University Soybean Breeding Program. NC Corn and Soybean Conference. January, 2010. New Bern, NC. Carley, D., T. Rufty, S. Sermons, L. Vance, D. Bowman and W. Shi. Carbon storage under bermudagrass fairways in the Southeast. ASA Annual Meeting, Long Beach, CA. Carlson, C., and A.J. Cardinal. Quantitative trait locus mapping for protein content in soybean using two genetically connected populations. ASA-CSSA-SSSA International Annual Meetings. October 31  November 3, 2010. Long Beach, CA. Abstract #187-13: http://a-c-s.confex.com/crops/2010am/webprogram/Session7609.html Carlson, C., and A.J. Cardinal. The effects of glycinin and beta-conglycinin subunit genes on the protein content of soybean. 13th Biennial Conference of the Cellular and Molecular Biology of the Soybean. August 8-11, 2010. Durham, NC. Abstract #26: http://continuingeducation.ncsu.edu/opd/MCBS/breeding.html Carlson, C., V.R. Pantalone, D.L. Hyten, J.M. Chaky, S. Wang, A.J. Cardinal. Quantitative trait locus mapping for protein content in soybean using two genetically connected populations. ASA-CSSA-SSSA International Annual Meetings. October 31  November 3, 2010. Long Beach, CA. Carlson, C., V.R. Pantalone, S. Wang, A.J. Cardinal. Genomic regions contributing to protein content in soybean. 13th Biennial Conference of the Cellular and Molecular Biology of the Soybean. August 8-11, 2010. Durham, NC. Chapin, J.W., J.S. Thomas, T.G. Isleib, F.M. Shokes, W.D. Branch, and B.L. Tillman. 2010. Continued evaluations of virginia-type peanut lines for resistance to late leaf spot, stem rot, and spotted wilt disease. 2010. Proc. Amer. Peanut Res. Educ. Soc. 41: 74-75. (On-line at www.apresinc.com/Proceedings.html). Copeland, S.C., T.G. Isleib, S.R. Milla-Lewis, B.B. Shew, J.E. Hollowell, H.E. Pattee, T.H. Sanders, L.L. Dean, K.W. Hendrix, M. Balota, and J.W. Chapin. 2010. Release of Bailey virginia-type peanut cultivar. Proc. Amer. Peanut Res. Educ. Soc. 42: 59-60. (On-line at www.apresinc.com/Proceedings.html). Copeland, S.C., T.G. Isleib, S.R. Milla-Lewis, B.B. Shew, J.E. Hollowell, H.E. Pattee, T.H. Sanders, L.L. Dean, K.W. Hendrix, M. Balota and J.W. Chapin. 2010. Release of Sugg virginia-type peanut cultivar. Proc. Proc. Am. Peanut Res. Educ. Soc. 42: 81-82. (On-line at www.apresinc.com/Proceedings.html) Dewey, R., R. Lewis, S. Bowen and L. Bush. Minimizing the nornicotine content in tobacco through combining mutant nicotine demethylase genes. 44th Tobacco Workers Conference. Lexington, KY. Jan. 2010. Dewey, R.E. Biotechnology-based approaches toward harm reduction in tobacco. NUTECH AgBiotech Industry Roundtable. Research Triangle Park, NC. Oct. 2010. Dewey, R.E. Using biotechnology to produce new tobacco varieties in an FDA regulated environment. AgBiotech Opportunities for Farmers and Growers. Clinton, NC. Dec. 2010. Dewey, R.E., R.S. Lewis, S.W. Bowen and L.P. Bush. Development of tobacco varieties with ultra-low levels of nornicotine. 64th Tobacco Science Research Conference. Hilton Head, SC. Oct. 2010. Friend, S.A., D. Quandt, S.P. Tallury, H. T. Stalker and K.W. Hilu. 2010. Species and genome relationships in Arachis: A molecular phylogeny. Proc. Amer. Peanut Res. Educ. Soc. 42:23-24. Gao, J., Y. Wang, T. Werner, L. Cardwell, J.P. Murphy, G. Brown-Guedira, C. Griffey, Y. Dong, and J. Costa. 2010. Mapping scab resistance in the winter wheat line MD01W233-06-1. In: S. Canty, A.Clark, A. Anderson-Scully, D. Ellis, D. Van Sanford (Eds.). Proceedings of the National Fusarium Head Blight Forum. 2010 Dec 7-9. Milwaukee, WI. Lexington, KY: University of Kentucky. p. 138-141. Isleib, T.G., S.C. Copeland, and S.R. Milla-Lewis. 2010. Genetic gain for pod yield in the North Carolina State University peanut breeding project. Proc. Am. Peanut Res. And Ed. Soc. 42: 79. Jones, E., H.T. Stalker, S. Tallury, S. Milla-Lewis, D. Petrik, and S. Knapp. 2010. Simple Sequence Repeat marker variability among Arachis species. Proc. Amer. Peanut Res. Educ. Soc. 42:53. Kimball, J.A., M.C. Zuleta, and S.R. Milla-Lewis. 2010. Transferring informative cereal SSRs to warm-season turfgrasses for germplasm characterization and evaluation. Proceedings of the Agronomy Society of America International Annual Meetings. Long Beach, CA. Oct 31 - Nov 4. Kuraparthy V, and D. Bowman. 2010. Introgressive breeding for developing thrips tolerance in upland cotton. Annual meetings of the Crop Science Society of America. Long Beach, CA. October 31-November 4. Kuraparthy V., P. Tyagi et al. 2010. Dissecting the components of hybrid vigor associated lint yield in cotton. Annual meetings of the Crop Science Society of America. Long Beach, CA. October 31-November 4. Kuraparthy V., S. Sood, and B.S. Gill. 2010. Molecular genetic description of the cryptic wheatAegilops geniculata introgression carrying rust resistance genes Lr57 and Yr40 using wheat ESTs and sytnteny with rice. PAG XVIII Abstr P283. Kuraparthy V., S. Sood, G.L. Brown-Guedira, and B.S. Gill. 2010. Development of PCR assays and marker-assisted transfer of rust resistance genes Lr57/Yr40 and Lr58 into adapted winter wheats. PAG XVIII Abstr P287. Lewis, R.S. and R.E. Dewey. Genetics-based modification of tobacco chemistry in a regulatory environment. 64th Tobacco Science Research Conference. Hilton Head, SC. Oct. 2010. Li, D., R. Lewis, A. Jack, R. Dewey, S. Bowen. and R. Miller. Development of dCAPS markers for CYP82E4 and CYP82E5 gene mutants reducing nicotine to nornicotine conversion in tobacco. 44th Tobacco Workers Conference. Lexington, KY. Jan. 2010. Lopez, H., S. Bowen, R. Lewis, L. Bush and R. Dewey. Identification and generation of tobacco plants possessing mutations in key genes of the alkaloid biosynthetic pathway. 44th Tobacco Workers Conference. Lexington, KY. Jan. 2010. Maloney, P.V., J.H. Lyerly, D.R. Wooten, J.M. Anderson, D.P. Livingston III, G. Brown-Guedira, D. Marshall, and J.P. Murphy. 2010. New advances in marker assisted selection for winter hardiness in oats. In: Proceedings of the American Oat Workers Conference. April 18-24, 2010. Baton Rouge, LA. Louisiana State University. p. 39. Milla-Lewis, S.R., K.R. Harris, M.C. Zuleta, B.M. Schwartz, and W.W. Hanna. 2010. Assessing genetic diversity in centipedegrass germplasm with sequence-related amplified polymorphism (SRAP) markers. Proceedings of the XVIII Plant and Animal Genome Conference. San Diego, CA. Jan,09-13. (available at http://www.intl-pag.org/18/abstracts/P03a_PAGXVIII_123.html ). Milla-Lewis, S.R., M.C. Zuleta, and T.G. Isleib. 2010. Assessment of genetic diversity changes in U.S. Runner-type peanut cultivars released between 1943 and 2009 using simple sequence repeat (SSR) markers. Proc. Am. Peanut Res. And Ed. Soc. 42: 21. Milla-Lewis, S.R., M.C. Zuleta, G.A. Van Esbroeck, K.H. Quesenberry, and K.E. Kenworthy. 2010. Molecular and Cytological Assessment of Genetic Diversity in Stenotaphrum Germplasm. Proceedings of the Agronomy Society of America International Annual Meetings. Long Beach, CA. Oct 31 - Nov 4. Murphy, J.P., and R. Navarro. 2010. The 2009-10 Southern Uniform Winter Wheat Scab Nursery. In: S. Canty, A. Clark, A. Anderson-Scully, D. Ellis, D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum. 2010 Dec 7-9. Milwaukee, WI. Lexington, KY: University of Kentucky. p. 154-158. Nagy, E., Y. Guo, S. Khanal, C. Taylor, S. Knapp, H.T. Stalker, P. Ozias-Akins, and N. Nielsen. 2010. Developing a high-density molecular map of the A-Genome species A. duranensis. Proc. Amer. Peanut Res. Educ. Soc. 42:25. Pattee, H.E., T.G. Isleib, S.C. Copeland, and S.R. Milla-Lewis. 2010. Genetic gain for flavor in the North Carolina State University peanut breeding project. Proc. Am. Peanut Res. And Ed. Soc. 42: 90. Rowe, C.E., V.J. Vontimitta, T.G. Isleib and S.R. Milla-Lewis. 2010. Identification of a QTL Associated with Reduced Post-Harvest Aflatoxin Accumulation in Peanut (Arachis hypogaea L.). Proc. Am. Peanut Res. And Ed. Soc. 42: 85. Shoots, J., M. Guttieri, F. Kolb, J. Lewis, A. McKendry, H. Ohm, C. Sneller, M. E. Sorrells, E. Souza, D. Van Sanford, J. Costa, C. Griffey, S. Harrison, J. Johnson and J. P. Murphy. 2010. Development and distribution of male-sterile facilitated recurrent selection populations. In: S. Canty, A.Clark, A. Anderson-Scully, D. Ellis, D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum. 2010 Dec 7-9. Milwaukee, WI. Lexington, KY: University of Kentucky. p. 165-166. Villegas Chirinos, F., Isleib, T.G., Knapp, S.J., and Milla-Lewis, S.R. 2010. Molecular mapping of QTL for early maturity in peanut. Proceedings of the Agronomy Society of America International Annual Meetings, Long Beach, CA. Oct 31 - Nov 4. Wang, M.L., H.T. Stalker, and R.N. Pittman. 2010. Assessment of oil content and fatty acid variability in peanut wild relatives. Proc. Amer. Peanut Res. Educ. Soc. 42:42. Zuleta, M.C., and S.R. Milla-Lewis. 2010. Use of AFLP markers for assessment of genetic diversity in St. Augustine grass germplasm. Proceedings of the International Conference on green plant breeding technologies, Vienna, Austria. Feb 02-05. Oklahoma Anderson, J.A., and Y.Q. Wu. 2011. Freeze tolerance of forage bermudagrasses. Grass and Forage Science (Accepted). Todd, J., Y.Q. Wu, Z. Wang, and T. Samuels. 2011. Genetic diversity in tetraploid switchgrass revealed by AFLP marker polymorphisms. Genetics and Molecular Research (Accepted). Wang, Y.W., T. Samuels, and Y.Q. Wu. 2011. Development of 1030 genomic SSR markers in switchgrass. Theoretical and Applied Genetics 122: 677-686. DOI 10.1007/s00122-010-1477-4. Wu, Yanqi, Dennis L. Martin, Zan Wang, Chengcheng Tan, Tim Samuels. 2011. Microsatellite molecular markers accurately identify clonal turf bermudagrass cultivars. USGA Turfgrass and Environmental Research Online. 10 (9):1-7. Bartley, L., Y.Q. Wu, A. Saathoff, and G. Sarath. 2011. Switchgrass genetics and breeding challenges. In M. Saha (ed.) Biomass Crops: Breeding and Genetics. Wiley-Blackwell. (In submission) Wu, Y.Q., and J.A. Anderson. 2011. Genetic improvement of cold hardiness in bermudagrass. pp 851-865. In M. Pessarakli (ed.) Handbook of Plant and Crop Stress (3rd edition). Taylor & Francis Group, CRC Press, Boca Raton, FL. Wu, Y.Q. 2011. Cynodon L.C. Richard. pp 53-71. In C. Cole (Ed.) Wild Relatives of Millets and Forage GrassesWealth of Wild Crop Relatives: Genetic, Genomic & Breeding Resources. Springer. South Carolina Arno, J., Gabarra, R., Liu, T.-X., Simmons, A.M., Gerling, D. 2010. Natural Enemies of Bemisia tabaci: Predators and Parasitoids. pp. 385-421. In Bemisia: Bionomics and Management of a Global Pest, P.A. Stansly, S.E. Naranjo (eds.). Springer Dordrecht-Heidelberg-London-New York. pp. 385-421. Baugher, T.A., J. Schupp, K. Ellis, J. Remcheck, E. Winzeler, R. Duncan, S. Johnson, K. Lewis, G. Reighard, G. Henderson, M. Norton, A. Dhaddey, and P. Heinemann. 2010. String blossom thinner designed for variable tree forms increase crop load management efficiency in trials in four United States peach-growing regions. HortTechnology 20(2):409-414. Black, B.L., D. Drost, T. Lindstrom, J. Reeve, J. Gunnell and G. L. Reighard. 2010. A comparison of root distribution patterns among Prunus rootstocks. J. American Pomological Society 64 (1): 52-62. Campbell, B.T., S. Saha, J. Jenkins, W. Park, R. Percy, J. Frelichowski, C. Mayee, V. Gotmare, D. Dessauw, M. Giband, X. Du, Y. Jia, G. Constable, S. Dillon, I. Abdurakhmonov, A.A. Abdukarimov, S. Rizaeva, A.A. Abdullaev, P.A.V. Barroso, J.G. Padua, L.V. Hoffman, and L. Podolnaya. 2010. Status of the global cotton germplasm resources. Crop Sci. 50:1161-1179. Dowd, M.K., D.L. Boykin, W.R. Meredith, Jr., B.T. Campbell, F.M. Bourland, J.R. Gannaway, K.M. Glass, and J. Zhang. 2010. Fatty acid profiles of cottonseed cultivars from the National Cotton Variety Trials. J. of Cotton Sci. 14:46-55. Fang, G.-C., Blackmon, B. P., Henry, D. C., Staton, M. E., Saski, C. A., Hodges, S. A., Tomkins, J. P., and Luo, H. 2010. Genomic tools development for Aquilegia: Construction of a BAC-based physical map. BMC Genomics 11:621. Farnham, M.W. 2010. Glossy and Nonglossy Near-isogenic Lines USVL115-GL, USVL115-NG, USVL188-GL and USVL188-NG of Broccoli Derived from Doubled Haploids. HortScience. 45:660-662. Farnham, M.W., Grusak, M.A. 2010. Mineral Concentration of Broccoli Heads in relation to Year of Cultivar Release. HortScience. 45:S282-S283 (Abstr.) Farnham, M.W. 2010. Breeding Vegetables Adapted to High Temperatures: A Case Study with Broccoli. HortScience. 45:S18 (Abstr.) Fery, R.L. 2010. An Overview of the Current Status of Southernpea Breeding Programs in the United States. HortScience. 45(4):504. Fery, R.L., Thies, J.A. 2010. PA-559, a Root-knot Nematode Resistant, Red-fruited, Habanero-type Pepper. HortScience. 45(5):822-823. Fery, R.L., Thies, J.A. 2010. PA-560, A Root-Knot Nematode Resistant, Yellow-fruited, Habanero-type Pepper. HortScience. 48(8):S184-S185. (Abstr.) Harris, K.R., Ling, K., Levi, A., Wechter, W.P. 2010. Identification and Utility of Markers Linked to the Zucchini Yellow Mosaic Virus Resistance Gene in Watermelon. Plant and Animal Genome Conference Proceedings. January 9-13, 2010, P-215. Harris, K.R., Wechter, W.P., Levi, A. 2010. Isolation and genetic mapping of NBS-LRR disease resistance gene analogs in watermelon. Plant and Animal Genome XVIII Conference, January 9-13, 2010, San Diego, CA. Harris-Shultz, K.R., Wechter, W.P., Levi, A. 2010. Linkage Mapping of NBS-LRR Disease Resistance Gene Analogs in Watermelon. HortScience. 45:510. Harrison Jr, H.F., Fery, R.L., Thies, J.A., Smith, J.P. 2010. Notice of Release of US-1136, US-1137, and US-1138 Cowpea Gerplasm Lines with Potential For Use As A Cover Crop. Germplasm Release. U.S. Department of Agriculture, Agricultural Research Service, Washington, D.C. 20350, January 4, 2010. Harrison Jr, H.F., Fery, R.L., Thies, J.A., Smith, J. 2010. US-1136, US-1137, and US-1138 Cowpea Germplasm Lines for Use as a Cover Crop. HortScience. 48(8):S281-S282. (Abstr.) Harrison Jr, H.F., Kousik, C.S., Levi, A. 2010. Tolerance to the Herbicide Clomazone in Watermelon Plant Introductions. HortScience. 45:510. Jackson, D.M., Bohac, J., Thies, J.A., Harrison Jr, H.F. 2010. `Charleston Scarlet- Sweetpotato. HortScience. 45:306-309. Jackson, D.M. 2010. Destructive and Useful Insects in Sweetpotato Breeding Nurseries. HortScience. 45:505. Jimenez, S., G. L. Reighard and D. G. Bielenberg. 2010. Gene expression of DAM5 and DAM6 is suppressed by chilling temperatures and inversely correlated with bud break rate. Plant Molecular Biology 73:157-167. Jiménez S, Z. Li, G.L. Reighard, and D.G. Bielenberg. 2010. Identification of genes associated with growth cessation and bud dormancy release using a dormancy-incapable tree mutant. BMC Plant Biology 10(25). Ke, P. C., Lin, S., Reppert, J., Rao, A., and Luo, H. (2010) Uptake of nanoparticles by mammalian cells and plants. In: Handbook of Nanophysics  Nanomedicine and Nanorobotics, Sattler, K. D. (ed), Taylor&Francis Group (CRC Press), Boca Raton, FL, pp 30:1-15. Keinath, A.P., Fillippeli, E., Hausbeck, M.K., Kousik, C.S. 2010. Baseline Sensitivity to Fluopicolide in Phytophthora capsici Isolates from the Eastern United States. Phytopathology. 100:S201. Kousik, C.S., Thies, J.A. 2010. Response of U.S. Bottle Gourd (Lagenaria siceraria) Plant Introductions (PI) to Crown Rot caused by Phytophthora capsici. Phytopathology. 100:S65. Tennessee Allen, F., V. Sykes, R. Johnson and J. Zale. 2011. An ideotype for selection among F1 half-sibs of switchgrass. Am. Soc. of Agronomy. Abstracts, CD. Johnson, R.D., F.L. Allen, C.E Sams, V.R. Pantalone and A. M. Saxton. 2010. Effects of forced and natural leaflet orientation on transpiration rates and other physiological traits in soybean. Am. Soc. of Agronomy. Abstracts, CD. Johnson, R., F. Allen, C. Sams, V. Pantalone and A. Saxton. 2009. Hydraulic Conductivity and Transpiration Rates of Soybean Plants Differing in Leaflet Orientation and Root Morphology. American Society of Agronomy Abstracts, CD. Fallen, B.D., V. R. Pantalone, C. E. Sams, D. A. Kopsell, Vaughn, S.F., and Moser, B.R.. 2011. Effect of Soybean Oil Fatty Acid Composition and Selenium Application on Biodiesel Properties. J. Am. Oil Chem. Soc. DOI 10.1007/s11746-010-1746-z. Jeong-Dong Lee, Melissa Woolard, David A. Sleper, James R. Smith, V. R. Pantalone, Catherine N. Nyinyi, Andrea Cardinal, and J. Grover Shannon. 2009. Environmental Effects on Oleic Acid in Soybean Seed Oil of Plant Introductions with Elevated Oleic Concentration. Crop Sci. 49:1762-1768. Virgin Islands Zimmerman, T.W. and N. Burnett. 2010 Influence of sucrose concentration on long-term sweet potato cultures. Proc. 46th Caribbean Food Crops Society. 46:74-78. Igwebuike, C., T.W. Zimmerman, 2010. Detecting viruses of sweet potato in the Virgin Islands. Twelfth Annual UVI Fall Research Symposium. 12:29. Matthew, K., T.W. Zimmerman, 2010. Seed germination in nine varieties of sorrel. Twelfth Annual UVI Fall Research Symposium. 12:33. Virginia S.A. Friend, D. Quandt, S.P. Tallury, H.T. Stalker, and K.W. Hilu (2010) Species, genomes, and section relationships in the genus Arachis (Fabaceae): a molecular phylogeny. Plant Systematics and Evolution 290:185-199
Log Out ?

Are you sure you want to log out?

Press No if you want to continue work. Press Yes to logout current user.

Report a Bug
Report a Bug

Describe your bug clearly, including the steps you used to create it.