SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

TAC Members: Florida (University of Florida) Ann Blount (paspalum@ufl.edu) (for Kevin Kenworthy) Guam (University of Guam) Mari Marutani (marutani@uguam.uog.edu) Kentucky (University of Kentucky) Timothy D. Phillips (tphillip@uky.edu) North Carolina (N.C. State Univ) Tom Stalker (tom_stalker@ncsu.edu) Oklahoma (Oklahoma State University) Yanqi Wu (yanqi.wu@okstate.edu) South Carolina (Clemson University) Emerson R. Shipe (eshipe@clemson.edu) Southern Assoc. Ag.Exp.Sta.Directors Gerald Arkin, Administrative Advisor (garkin@uga.edu) Griffin PGRCU Staff: USDA-ARS-PGRCU, Griffin, GA Gary A. Pederson, Research Leader (gary.pederson@ars.usda.gov) Noelle Barkley (elle.barkley@ars.usda.gov) Other Attendees: USDA-ARS, College Station, TX James Frelichowski (james.frelichowski@ars.usda.gov) USDA-ARS, Beltsville, MD Peter Bretting (peter.bretting@ars.usda.gov) USDA-NIFA, Washington, DC Ann Marie Thro (athro@nifa.usda.gov)

The meeting was called to order at the Ramada Geneva Lakefront Hotel at 1:00 pm on July 27, 2010 by Tim Phillips, Chair. Those present introduced themselves. Due to weather problems and flight delays, Don LaBonte (LA), Paul Raymer (GA), and Tom Zimmerman (Virgin Islands) were unable to make it to Geneva in time to attend the S-009 meeting. Ann Blount agreed to serve as acting S-009 Secretary and take minutes for Paul Raymer. Dr. Gerald Arkin, the administrative advisor for the S-009 RTAC, welcomed the group to the S-009 meeting in Geneva. He gave an overview about the role of the CGCs, citing their responsibilities. He stressed the need to report back to the state administrators about the PI collections and GRIN if there are any short comings or needs. He mentioned the importance of letting clientele know where plant germplasm comes from and inform the public more about the role of NPGS in our presentations and writings. The minutes from the 2009 meeting were approved as previously circulated and posted on the website. There were no changes to the agenda. Two new Crop Germplasm Committees (CGC), Medicinal Plants CGC and Specialty Nut Crops CGC, have been created and are being organized. Ann Marie Thro gave a NIFA update and stressed that we remember to recognize and cite that Hatch funding was used to support various research projects. Hatch funding support should be mentioned in any relevant CRIS reporting, resulting publications, annual reports, and presentations. Also it should be reported in plant registration articles in the Journal of Plant Registration and Journal of Horticulture Science where Hatch funding supported the development of cultivars or germplasm. Hatch funding will be flat this next year. AFRI funding is up 20% and genetic resources will be up under AFRI. The only way to get Hatch funding increased is to promote the fact that Hatch funds directly support our programs. We need to ask the editors of the various journals to remind authors to mention Hatch funding support where applicable. Ken Quesenberry might be willing to contact the CSSA editorial chair and suggest this to authors. Ann Marie also mentioned CRIS reporting, particularly the outcome and impact sections. The non-technical summary is a mini publicity effort and needs to be taken more seriously; especially noting how the research involves other researchers and how the outcome impacts clientele/farmers. Soon reporting will be done through REEport within a two year period. Gary Pederson discussed the Plant Genetic Resources Conservation Unit accomplishments over the past year. This included a general discussion about what is housed at the Griffin, GA location. There was some discussion of seed storage at -18 C and a building addition to the ARS Seed Processing building that will house a new 4 C cold room for seed storage at Griffin. The addition of this cold storage room will enable previous 4 C cold storage to become -18 C storage enabling bulk seed of the entire Griffin collection to be housed at -18 C. Gary also reiterated Ann Marie Thros concern about mentioning the Hatch funding that we use to support our research and also that we should recognize NPGS and its impact on our research. More specifically, we should indicate in publications and presentations what percent (%) NPGS germplasm contributed to the development of any new variety. Gary discussed changes in curation of collections located at Griffin. John Erpelding, sorghum curator at Mayaguez, Puerto Rico, has taken a new position at Stoneville, MS. Currently, Gary is serving as the acting sorghum curator. He also reported that Roy Pittman will become the Vigna curator as soon as a new peanut curator is hired. The plan is to complete the search and selection of a new peanut curator shortly after October 1, 2010. There was discussion about the amount of accessions that had low germination rates and how to better report that information at S-009 and CGC meetings. Tom Stalker encouraged more reporting of regeneration plans from the curators, especially regeneration plans from the new peanut curator. Gary noted that he will have the curators present their regeneration plans to the S-009 committee at the meeting next year in Griffin. Noelle Barkley reported on Detection of sweetpotato leaf curl virus (SPLCV) in the U.S. germplasm collection via real-time PCR. Mari Marutani asked how difficult the assay was to run and discussed the possibility of learning how to run the assay during the next S-009 meeting in Griffin. She would like to have a better understanding of the viral status of sweetpotatoes in Guam. Peter Bretting discussed GRIN Global and stated that it is 90% accomplished. He stated that Griffin will have a modest increase in its budget. Overall budget increases of $40 million dollar will mostly go into salaries, cost of living increases, and STEP increases across the agency. Also, the Foreign Affairs committee has to approve the germplasm treaty and there is still a hold up. State reports for Florida, Guam, Kentucky, North Carolina, Oklahoma, and South Carolina were presented orally (written reports at the S-009 website). Written reports were distributed for Alabama, Georgia, Hawaii, Louisiana, Texas, and Virginia. James Frelichowski reported on the status of the cotton germplasm collection at College Station, TX. The 2011 S-009 meeting will be held in Griffin, GA, on August 2-3, 2011 starting at 1:00pm on Tuesday, August 2nd. The incoming S-009 Chair will be Paul Raymer. Kevin Kenworthy was nominated to be the incoming secretary. There were no other nominations and Kevin was elected. S-009 members were encouraged to attend the joint RTAC/CGC Chairs/PGOC session tomorrow morning and the tour at Geneva in the afternoon. The meeting was adjourned at 5:00pm.

Accomplishments

The complete accomplishments section for the 2010 S-009 annual report is located at the S-009 website (www.ars.usda.gov/Main/docs.htm?docid=9514). A summary of the accomplishments from the Griffin location and S-009 cooperators is listed below. USDA, ARS, Plant Genetic Resources Conservation Unit: A total of 90,668 accessions of 1,546 plant species were maintained in the Griffin plant genetic resources collection. Over 87.9% of accessions were available for distribution to users and over 96.3% were backed up for security at a second location. Bulk seed samples for 62,524 accessions were maintained at -18 C for long-term storage with seed of the remaining accessions stored at 4 C. A total of 40,449 seed and clonal accessions in 899 separate orders were distributed upon request to scientists and educators in 50 U.S. states and 38 foreign countries. Acquisitions made to the collection included 354 sorghum, 125 peanut, 70 annual clover, 68 warm-season grasses, and 22 vegetable accessions. A plant collection trip was conducted in northern Florida for native switchgrass germplasm. Seed regenerations and characterization were conducted on 275 peanut, 103 warm-season grass, 70 pepper, 346 legume, new, and misc. crop, 42 annual clover, and 8 cucurbit accessions. New regeneration techniques were developed for Hibiscus and Bituminaria species. Over 300 pepper accessions were grown in the field for characterization and recording of digital images. Digital images of cowpea, sorghum, pepper, and warm-season grass accessions, and pepper root rot and watermelon root knot nematode data were added to the Germplasm Resources Information Network (GRIN). Long-term maintenance of 543 wild peanut and 410 warm-season grass accessions was continued in the greenhouse. Over 30 warm-season grass accessions and 750 sweetpotato accessions were maintained in tissue culture with eight replications of each sweetpotato clone. Virus screening was completed on the sweetpotato collection for sweetpotato leaf curl virus. Germination testing has been completed for 60,207 accessions (over 67% of collection) since 2002. A genotyping technique was developed to detect high oleic acid peanuts and will be useful in evaluating segregating populations to identify progeny with the desirable high oleic acid trait. Molecular analysis and ploidy level determinations are underway for newly acquired switchgrass germplasm from Florida. Basic descriptors were collected on the entire U.S. bamboo collection. The oil content was determined for the entire U.S. castor bean (over 1,000 accessions) and okra (over 1,200 accessions) collections and the U.S. sesame collection is currently being evaluated for oil content. Fifty castor bean accessions are being grown in the field to verify oil content and determine relationships with fatty acid composition. Okra accessions varying in oil content will be evaluated for fatty acid composition. In collaboration with other ARS scientists in Dawson, GA, the peanut mini-core collection was grown in the field for morphological evaluation, is being biochemically analyzed in the lab, and is being genotyped with 84 genetic markers. In collaboration with Kansas State University scientists, association analysis will be conducted to identify association of genetic markers with useful peanut traits. Alabama: Current work focuses on evaluation, utilization and breeding of sunn hemp and sericea lespedeza species. Sunn hemp germplasm is being used for development of cultivars for the continental US. Sericea lespedeza is being evaluated for control of gastrointestinal parasites in ruminants and for condensed tannin content. A new low-growing sericea lespedeza will be evaluated for road-side use with colleagues at Auburn, GA, LA, AR, and USDA at several locations. Current work focuses on evaluation, utilization and breeding of upland cotton. Cotton accessions continue to be evaluated to resistance to reniform nematode, but we have not been able to find adequate resistance in materials we have evaluated. We also continue to evaluate accessions for resistance to heat and drought, and have begun the introgression of some putative heat tolerant accessions into adapted germplasm for further study. We are also conducting a study on the impact of exotic germplasm introgression on cotton yield and fiber quality traits. Florida: Florida remained active in 2009 for plant genetic resources distribution and use, with 28 different individuals requested materials from 25 different genera of plants and a total of 510 unique PIs distributed. Affiliation of individuals obtaining materials included University of Florida scientists, USDA scientists, private research organizations, private citizens, and public schools. Most individuals who responded to a request for information indicated a high level of satisfaction with materials provided and appreciation for the availability of the germplasm. Users included public and private plant breeding companies, NGOs, and private individuals. Georgia: Thirty separate requests for plant germplasm were made to the S-9 unit by the citizens of Georgia during 2009. As a result of these requests, a total of 723 plant accessions were supplied to University scientists, USDA scientists, consultants, seed companies, gardeners, and individuals. The most requested crops were sorghum, castor, watermelon, peanut, paspalum and peppers. The Institute of Plant Breeding, Genetics, and Genomics (IPBGG), officially established at UGA in 2008, has 20 faculty members and six affiliated members. Georgia plant breeding programs supply new crop cultivars and associated technologies to our agricultural sector and rely heavily upon the plant materials maintained within the S-9 unit. UGA currently has active cultivar development programs in soybean, peanut, small grains, cotton, turf grass, forages, blueberry, pecan, fruits, vegetables, and numerous ornamental crops that frequently utilize the plant genetic resource collections. In addition, research programs in crop science, horticulture, plant pathology, entomology, and other disciplines utilize the genetic resources of the S-9 unit in both basic and applied research projects designed to address the needs of Georgia agriculture. The S-9 unit remains a critical component of our research and cultivar development programs. Guam: The University of Guam continued to maintain local lines of sweetpotato and chili pepper. Seeds of sunnhemp originated from Taiwan were collected for future field evaluation with other lines. A chili pepper cultivar was collected locally as a new entry. Seeds of Jatropha curcas and Calophyllum inophyllum are being collected for a research project on evaluation of potential biodiesel oil production in Guam. A local line of pumpkin was planted for seed collection at Guam Agricultural Experiment Station Yigo Farm. Nine leafy lettuce cultivars were evaluated at the Yigo Farm during the rainy season. A two-year field trial of five yardlong-bean selections in three different soils, Pulantat clay soil, Akina silty clay soil and Guam cobbly clay loam soil in Guam was summarized for dissemination of the results. Four cultivars and a germline obtained from a local farm were tested. The study indicated that yield potential of yardlong bean would be improved by selection of planting season, location, and cultivar. Hawaii: Germplasm requested in 2009 included Dactyloctenium, Ipomea batatas, Megathyrsus, and Pennisetum. Dactyloctenium australe (sweet smother grass) seeds were received for evaluation as a shade tolerant ground cover in macadamia orchards by the University of Hawaii (UH) and two commercial macadamia growers. Plantlets of 86 sweetpotato varieties were received by a UH cooperative extension agent and will be evaluated for their productivity, insect and disease tolerance and commercial potential. Pennisetum purpureum and Megathyrsus maximus accessions are being evaluated at the UH with wild accessions from Hawaii for biofuels production in different environments. Collaborative research with Dr. Lisa Keith, USDA-ARS Plant Pathologist, has determined that Phytophthora tropicalis is the cause of macadamia quick decline (MQD), a problem which has been responsible for death of a large number of mature macadamia trees in Hawaii. Experimental results show that trunk injections with Fosphite fungicide can be effective in preventing or delaying return of MQD symptoms if trees are treated when signs of MQD are first observed. A study to monitor longan (cv. Biew Kiew) fruit growth, fruit quality, and the pattern of moisture and nutrient accumulation during fruit development was initiated. A heavy infestation of armored scales on rambutan tree leaves and fruits was observed at the University of Hawaii Waiakea Agricultural Research Station. A presentation to growers and its impact on fruit export was made to enable growers to identify the pest. Growers were also alerted that a number of bio-control insects were found feeding and that chemical control options are available with buprofezin. Kentucky: Todd Pfeiffer continues to work on sweet sorghum breeding, but much less now that he is the chairman of the Department of Plant and Soil Sciences at the University of Kentucky. Mike Barrett has picked up his sweet sorghum biomass research project. Sadly, Dr. Morris Bitzer passed away from a sudden illness in the spring. He has been an avid advocate of sweet sorghum and had retired from a grain crops extension position at U.K. Tim Phillips has planted a trial to evaluate the winter hardiness in KY of some warm season grasses in the S9 collection, as well as a breeding nursery for several native warm-season grasses. In 2009, he obtained some finger millet accessions and a few other African species to investigate performance of C4 gluten-free grain crops. Many of the switchgrass accessions in a selection nursery appear to have become infected with a virus. Other germplasm sent to KY in 2009 includes sorghum, pepper, watermelon, clovers, peanut, Ipomoea, and vinca. In 2009, more university researchers requested accessions than private companies or individuals, with three institutions housing these researchers (University of Kentucky, Kentucky State University, and Kentucky Wesleyan University). Louisiana: Germplasm from the bamboo collection is routinely evaluated by a nursery in Louisiana for commercial landscape applications. More accessions of Phyllostachys edulis, Phyllostachys vivax, Bambusa multiplex, and Bambusa textilis are needed in the collection. Sweetpotato accessions were requested to identify sources of resistance to Sweet Potato Leaf Curl Virus. Research on Ipomoea accessions include evolutionary ecology, molecular evolution and population genetics of genes involved in flower color, as well as the phylogenetic systematics of morning glories (species of the tribe Ipomoeae). Hibiscus accessions were requested to identify fatty acid profiles (as methyl esters) of repository plants to help confirm the identity of peaks observed in related Gossypium (cotton) samples. Legumes were examined for induced flavonoids/isoflavonoids (phytoalexins) in legumes for anticancer and antiobesity activities. Research has expanded to other legumes. Certain species of Teramnus make a phytoalexin called glyceollins that is produced in soybean and has anticancer activity. North Carolina: Faculty in the Crop Science and Horticultural Science Departments at NC State University conduct research on strawberry, blueberry, brambles, tree crops, ornamentals, maize, soybean, peanut, cotton, tobacco, small grains, turfgrasses, sweet potato, cucurbits, and other crops. NCSU is hiring a soybean breeder to replace Dr. Joe Burton when he retires within the coming year. Priorities are on incorporating disease and insect resistance, abiotic stress resistance, and quality factors into improved breeding lines and cultivars. Plant introductions are critical components of plant improvement programs, and NCSU scientists make use of germplasm maintained in the National Plant Germplasm System (NPGS). During the past year, 32 individuals received 459 entries to NC from the Griffin station, including 6 cultivated peanut, 212 Arachis species, 13 sorghum, 1 cowpea, 92 sweetpotato, 2 eggplant, 10 peppers, 3 clover, 2 Cucurbit spp., 2 watermelon and 116 warm season grasses. The U.S. collection of cultivated and wild Nicotiana species is maintained at NCSU and numerous seed requests are filled annually both to U.S. and international individuals and organizations. During the past year, both germplasm lines and cultivars have been released by the NC Agricultural Research Service including 7 maize inbreds, 2 oat inbreds, 1 pickling cucumber germplasm, 1 pickling cucumber cultivar, 3 redbud clones, 1 soybean cultivar, 1 soybean germplasm, 1 summersweet clone, 2 sweetpotato ornamental clones, 1 switchgrass population, 2 tomato cultivars, 3 tomato germplasms, 2 winter wheat cultivars, and 4 winter wheat germplasms. Oklahoma: Plant germplasm distribution records received from S-9 indicate that 352 plant accessions maintained at the Plant Genetic Resources Conservation Unit at Griffin, GA were distributed to organizations or individuals in Oklahoma the last year, from August 2009 through July 2010. The accessions were distributed in 21 respective requests. Respective annual plant germplasm distribution numbers for 2005, 2006, 2007, and 2008 were 597, 169, 295 and 431. The requested plant species in 2009 include sorghum (Sorghum bicolor) (214), sweetpotato (Ipomoea batatas) (81), Guar (Cyamopsis sp.) (33), squash (Cucurbita spp.), Okra (Abelmoschus sp.), vigna (Vigna spp.), switchgrass (Panicum virgatum) and Miscanthus (Miscanthus sp.) and other warm-season grasses, and legumes. Receivers of the plant accessions represent researchers of Oklahoma State University, the Noble Foundation, USDA-ARS laboratories, and individual Oklahomans. Puerto Rico: Eighteen quenepa (Melicoccus bijugatus) cultivars are in the fifth year of evaluation at Juana Díaz and Lajas. At Juana Díaz and Lajas, respectively, 48% and 22% of the trees fruited in 2009. Horticultural traits and fruit quality parameters (pH, TSS, acid, and TSS/acid ratio) were measured on the mandarin cultivars Encore, Murcott and Fallglo on five rootstocks in Corozal. At Isabela, a citrus germplasm collection was established in the screenhouse and citrus rootstocks are being propagated for new experiments to be established at Corozal and Adjuntas. Five hybrid and five open-pollinated Cubanelle-type pepper cultivars were evaluated from February to May. Twelve Mayaguezano-type mango clones on Banilejo dwarfing rootstock are being evaluated in the field at Lajas. A field planting of 14 guava accessions is being evaluated at Juana Díaz. Estela hybrid tannier production was evaluated using planting material of different source and size. Marketable yield was increased by 27% when planting material from main corm sections was used, compared with crowns. Cuerno de Arce plantain was evaluated with and without a chicken manure soil amendment. One hundred half-sib lines of Suresweet sweet corn are being evaluated in a replicated performance trial. After the second cycle of mass selection has been completed, a manuscript will be prepared for the formal release of this sweet corn variety as Suresweet 10. Cabezona pineapple clones HT1-N, HT2-N and I-B showed the best fruit characteristics (size, weight and brix). Germplasm requests in Puerto Rico in 2009 include 221 Citrullus sp., 1 Praecitrullus sp., and 16 Crotalaria sp. South Carolina: A total of 925 germplasm accessions were distributed by the Plant Genetic Resources Conservation Unit at Griffin, GA including 478 Vigna (cowpea), 6 Arachis (peanut), 309 Citrullus (watermelon), 13 Praecitrullus (watermelon), 16 gourd, 7 Luffa, 23 Cucurbit spp., 1 Solanum (eggplant), 3 Cymbopogon (lemon grass), 5 Catharanthus, 2 Cucurbita, 44 Ipomoea (sweetpotato), 2 Trifolium (clover), 1 Sesamum (sesame), 12 Capsicum (pepper), and 1 Abelmoschus (Okra) accession. In 2009, twenty-one elite lines and/or cultivars from the Clemson University soybean breeding program were tested as hosts for reniform nematode, Rotylenchulus reniformis. Elite lines SC06-687, SC05-642, SC01-803, and SC03-9093 had reproductive indices lower than the reniform resistant breeding line, SC02-208. USDA soybean germplasm accessions were evaluated for soybean rust (SBR) resistance in seven locations in the southern U.S. The objective is to identify and confirm PIs that exhibit resistance to rust isolates in multiple locations in order to provide soybean breeders with guidance in choosing parents for crosses made to develop rust-resistant soybean cultivars with competitive yields. Ninety-one genotypes across maturity groups III through IX were planted at Blackville, S.C. August 18, 2009, in three replications. A manuscript summarizing results of the PI screening effort has been accepted by Crop Science. Texas: Switchgrass research is in progress at Weslaco, TX using two PI lines (PI422016 and PI431575). Four accessions of Pennisetum purpureum (PI 316421, 410305, 410306, 410307) are in biomass production evaluations near College Station, TX. The Texas A&M Sorghum Improvement Program requests approximately 2,000 sorghum accessions annually. We screen these lines for agronomic potential and any other desirable traits that we may find. Some will be crossed to elite adapted lines to develop new breeding populations for selection. Three sorghum lines requested by Dr. Cleve Franks are currently being grown at Taft and Plainview for seed increases and evaluation for head smut, downy mildew and anthracnose resistance. Nineteen PI's of big hop clover (Trifolium campestre) were evaluated for adaptation to northeast Texas. Crimson clover, lablab and cowpea accessions are being evaluated. Peanut germplasm materials requested this past year were used to re-establish the lines in our germplasm collection at Stephenville and for use in making crosses to improve our cultivated varieties. The USDA/NRCS Plant Materials Center in Kingsville, Texas received collections in 2009 of 9 Illinois bundleflower (Desmanthus illinoensis), 27 purple bushbean (Macroptilium atropurpureum), 5 poormans friend (Stylosanthes viscosa) and 16 sunn hemp (Crotolaria juncea) accessions from S-009. Accessions of Aleppo chilies are in evaluations for seed and pepper production in Texas. Virginia: Germplasm from S-009 was acquired in Virginia both by academics for research purposes and from the public for trials in farms and gardens. Backyard gardeners conducted trials of eggplant, pepper, watermelon and cultivated peanut. Researchers at Virginia State University evaluated several new oilseeds (Brassica sp.) and legume crops (Sunn hemp, Guar, Lablab) for potential production. Another study at Virginia State University was conducted to test the market for niche and ethnic crops, e.g., jute seed and jute leaf that was test marketed in farmers markets around Richmond where it was received favorably by the Middle Eastern community and others aware of its culinary uses. Additional work will be conducted on two Solanum spp, four cowpea lines and Cleome, to test demand among local communities of African origin. Crotalaria brevidens (Sunn hemp) was studied for its potential as a biomass crop with the conclusion that it can fit well into short-rotation sustainable vegetable production systems in the Southeast. Peanut, pepper and watermelon accessions were used in research at the Universidad Nacional de Cuyo in Argentina in a differential host test to identify the race of nematodes (Meloidogyne spp.) and in hybrid development among solanaceous species. These activities document the distribution and utilization of plant genetic resources, a primary objective of the regional project, in Virginia. Virgin Islands: Sorrel was planted in November to determine the production potential of a late planted sorrel. Twenty varieties were evaluated which included local varieties as well as from the USDA Plant Genetic Resources Conservation Unit in Griffin, GA. Sorrel plots were established as on-farm trials in three locations. Late planting of Sorrel in November provided harvest in February through March. This extends the production potential of this crop from the traditional December through January. The late winter production was at a time when demand is great for the product which results in a higher market price for the farmer. Branch angle was variable among accessions with upright branching more favorable for harvest. Breeding has been initiated to develop day neutral lines with large dark red calyxes. Eleven virus-free varieties of sweetpotato were obtained in vitro from Griffin and micropropagated to generate plants for field trials. Sweetpotatoes were established in the field at three month intervals. The virus-free sweetpotatoes grew vigorously. Two varieties developed excessive vines. Four varieties had better tuberous root quality than locally grown varieties that farmers are using to increase production.

Impacts

  1. 1. The germplasm collection at Griffin, Georgia has increased to 90,668 accessions of 255 genera and 1,546 species. Currently, 96.3% of the collection is backed up at the National Center for Genetic Resource Preservation, Ft. Collins, CO, and 87.9% of the accessions are available for use by the research community. Backing up safely secures these plant genetic resources for future use by researchers and good availability provides users with a wide array of currently available germplasm.
  2. 2. In 2009, a total of 40,449 seed, tissue culture, and clonal accessions were distributed to users for their research use. All accessions were requested from the Griffin location directly by researchers and distributed in 899 orders to users in all 50 states and 38 foreign countries, with 18,444 accessions distributed to users in the S-009 states. Genetic resources maintained at the Griffin location provide a valuable resource for crop improvement research.
  3. 3. Seed quality is evaluated by germination testing and seed longevity is improved through -18 C storage. Germination tests were conducted on over 9,800 accessions in the last year with over 67% of the entire collection at Griffin tested for germination since 2002. The program continued to split seed samples of all accessions at Griffin with a small distribution sample maintained at 5 C and the bulk of each sample maintained at -18 C to maximize seed longevity. Currently, over 70% (over 62,500 accessions) of the entire Griffin collection has at least one sample in -18 C storage.
  4. 4. Characterization and evaluation of the genetic resources enables researchers to more efficiently utilize the Griffin germplasm collection. Digital images of cowpea, sorghum, chile pepper and warm-season grass accessions were taken. Variation for oil content was determined for the entire castor bean (>1,000 accessions) and okra (>1,200 accessions) collections. Virus screening was completed on the entire sweetpotato collection for sweetpotato leaf curl virus. Characterization for descriptors was conducted on chile pepper, warm-season grasses, peanut, misc. legume, new crop, annual clover, cucurbit, and other accessions. All evaluation, characterization, and image data was uploaded onto the GRIN database.
  5. 5. Additional impacts and future plans are noted within the complete 2010 S-009 annual report located at the S-009 website (www.ars.usda.gov/Main/docs.htm?docid=9514).

Publications

Plant Genetic Resources Conservation Unit Auld, D.L., Zanotto, M.D., Mckeon, T.A., Morris, J.B. 2009. Castor. Handbook of Plant Breeding. 4:317-332 Barkley, N.L., Chamberlin, K.D., Wang, M.L., Pittman, R.N. 2010. Real-Time PCR Genotyping using Taqman Probes to Detect High Oleic Acid Peanuts. American Peanut Research and Education Society Abstracts. Online DOI 10.1007/s11032-009-9338-z 25:541-548. Barkley, N.L., Chamberlin, K.D., Wang, M.L., Pittman, R.N. 2009. Development of a realtime PCR genotyping assay to identify high oleic acid peanuts (Arachis hypogaea L.). Molecular Breeding. DOI 10.1007/s11032-009-9338-z 25(3):541-548. Chen, Z., Wang, M.L., Barkley, N.L., Pittman, R.N. 2010. A Simple Allele-Specific PCR Assay for Detecting FAD2 Alleles in Both A and B Genomes of the Cultivated Peanut for High Oleate Trait Selection. Plant Molecular Biology Reporter. DOI 10.1007/S11105-010-0181-5. Chen, X., Wang, M.L., Holbrook Jr, C.C., Culbreath, A., Liang, X., Brennenman, T., Guo, B. 2010. Identification and characterization of a multigene family encoding germin-like proteins in cultivated peanut (Arachis hypogaea L.). Plant Molecular Biology Reporter. DOI 10.1007/s11105-010-0237-6 Harrison Dunn, M.L., Pinnow, D.L. 2009. Influence of Palea and Lemma Removal on Seed Viability in Long Term Storage of Paspalum spp.. ASA-CSSA-SSSA Annual Meeting Abstracts. Jia, J., Liu, C., Wang, D., Chao, S., Wang, M.L., Qi, X. 2010. Mike Gale and cereal genetics research in China. Molecular Breeding. 25:365-367 Mckinney, J.T., Nay, L.M., Dekoeyer, D., Reed, G.H., Wall, M., Palais, R., Jarret, R.L., Wittner, C. 2010. Mutation Scanning and Genotyping in Plants by High Resolution DNA Melting. Handbook of Plant Mutation Screening. pp 149-163. Morris, J.B. 2009. Assessment of anthocyanin and agronomic trait variation in some commonly used medicinal legumes. ASA-CSSA-SSSA Annual Meeting Abstracts. Morris, J.B. 2009. Morphological and reproductive characterization in Hyacinth bean, Lablab purpureus (L) Sweet germplasm with clinically proven nutraceutical and pharmaceutical traits for use as a medicinal food. Journal of Dietary Supplement. DOI:10.1080/19390210909070830 6(3):263-279 Morris, J.B. 2010. Morphological and reproductive characterization of guar genetic resources regenerated in Georgia, USA. Genetic Resources and Crop Evolution. 10.1007/s10722-010-9538-8. Pederson, G.A., Pinnow, D.L., Spinks, M. 2009. Establishing Germination Testing as a Priority in a Genebank. ASA-CSSA-SSSA Annual Meeting Abstracts. Wang, M.L., Chen, C.Y., Davis, J., Guo, B., Stalker, T., Pittman, R.N. 2009. Assessment of oil content and fatty acid composition variability in different peanut subspecies and botanical varieties. Plant Genetic Resources.doi: 10.1017/S1479262109990177. Wang, M.L., Morris, J.B., Raymer, P., Davis, J., Lowery, C., Pederson, G.A. 2009. Variation in Oil Content and Fatty Acid Composition in the US Castor Bean Germplasm. ASA-CSSA-SSSA Annual Meeting Abstracts. Wang, M.L., Zhu, C., Barkley, N.L., Chen, Z., Erpelding, J.E., Pederson, G.A., Yu, J. 2010. Assessment of Genetic Diversity and Population Structure of Sweet Sorghum Accessions in the U.S. Germplasm Collection. Plant and Animal Genome Conference. Xin, Z., Wang, M.L., Burow, G.B., Burke, J.J. 2009. Sorghum TILLING Population  A Community Resource for Sorghum Improvement[abstract]. ASA-CSSA-SSSA. November 1-5, 2009, Pittsburgh, Pennsylvania. Alabama Floriculture, Ornamental and Plant Biotechnology. Online ISSN 1749-0308 Terrill, T.H., G.S. Dykes, S.A. Shaik, J.E. Miller, B. Kouakou, G. Kannan, J.M. Burke, and J.A. Mosjidis. 2009. Efficacy of sericea lespedeza hay as a natural dewormer in goats: Dose titration study. Veterinary Parasitology 163:52-56. Wang, M. L., J.A. Mosjidis, J. B. Morris, Z. B. Chen, N. A. Barkley, and G. A. Pederson. 2009. 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