Brief Summary of Minutes of Annual Meeting

NC7 RTAC Agenda - July 27, 2010 - Ramada Geneva Lakefront, Geneva, NY 8:00 - 8:45 Wendy Wintersteen , Dean and Agr. Exp Station Director, Iowa State University; Welcome, NC7 business, Hatch funding 8:45  9:15 Peter Bretting, Office of National Programs, USDA-ARS, Beltsville, MD 9:15  9:30 Harry Danforth, USDA-ARS Midwest Area Assoc Director, Peoria, IL 9:30  10:00 Ann Marie Thro, USDA-NIFA ONP, Washington, DC 10:15 - 10:30 Candice Gardner, USDA-ARS, NCRPIS, Ames, IA: Update and budget presentation 10:30  12:00 Gary Kinard, Marty Reisinger, Mark Bohning , USDA-ARS-DBMU, Beltsville, MD: GRIN Global Overview/Public Demonstration/Discussion (with the CGC Chairs) 1:00 - 1:15 Gardner, continued 1:15  1:45 Curator Dave Ellis and Research Leader Dave Dierig, USDA-ARS- NCGRP, Ft. Collins, CO 1:45  2:00 Terry Isbell, USDA-ARS-NCAUR, Peoria, IL: Oilseed Research Update 2:00  4:15 RTAC Attending Member State Reports & Discussion: Attending members: Amy Iezzoni, Rick Hall, Kendall Lamkey, Grover Shannon, Mike Stamm, Dipak Santra, Jim Orf, Bill Tracy 4:15  4:30 Mark Widrlechner, USDA-ARS-NCRPIS, Ames, IA: Update on Ash and EAB status 4:30  5:00 Open discussion: NCRPIS budget, RTAC expectations, new action items, adjourn 8:00  9:00 Special Speaker: Susan Dworkin - The Metaphor in the Seed Bank: Helping the Public to Understand Agriculture

NCRPIS Accomplishments for 2010: The collection totals 51,393 accessions with 74% available for distribution and 79% backed up at Ft. Collins, CO. About 1,936 accessions were also backed up at the Svalbard Global Germplasm Vault. Over 516 new accessions were acquired, approximately equal to 1% of prior holdings. Of these, 332 were received from within the NPGS through exploration and transfer. New germplasm included ash, collected in order to respond to the Emerald Ash Borer threat; 115 oilseeds, primarily wild and cultivated Helianthus; two species of Amaranthus not previously held; wild populations of Aronia and Gymnocladus (Kentucky coffeetree); 178 maize lines, including 16 inbreds derived from teosinte donated by J. Doebley of the University of Wisconsin; 45 Daucus collected in AZ, CA, NM, OR, and WA. Original seed samples of the accessions are stored at -18 C to extend seed longevity, while the working collection is stored at is improved by storage in -18 C rather than 4 C. Viability tests were conducted on 2,570 accessions (5% of the NCRPIS collections). After-ripening progress in Calendula was documented in order to better understand loss of seed dormancy in cold storage over time. Alternative treatments to six week cold-stratification treatments were explored for breaking seed dormancy of wild Helianthus. Optimum germination conditions were experimentally determined for portions of the Thlaspi and Camelina collections, using a thermal gradient table. Cryopreservation storage methods for ash buds were developed with ARS investigators in Ft. Collins, CO (NCGRP). A total of 26,651 items representing 13,226 accessions were distributed to fulfill 1,279 orders to 943 recipients worldwide. 2010 distributions were approximately equal to 2009 distributions. Approximately 72% of all distributions were domestic, and approximately ½ of these are to NC-7 Region researchers and educators. Some large requests included all of the available maize inbreds for a collaborative genotyping and multi-location phenotyping study which will contribute to understanding of collection diversity, Brassica and vegetables for disease resistance screening, and Helianthus for biomass/bioenergy evaluation and association mapping studies. Regeneration efforts in Ames and with collaborators resulted in harvest of 1,170 accessions. Five different insect pollinators were used to assist in pollination of 626 accessions. Honeybees were utilized for approximately ½ of the accessions, alfalfa leaf cutter bees for approximately ¼, and the remainder used bumblebees, Osmia bees, or flies. Multiple pollinators are needed for effective pollination of some accessions. Over 17,720 observations were entered in the GRIN database, as well as over 1,480 digital images. About 7,400 accessions were used for internal observation, evaluation and characterization for a wide array of descriptor information, viability testing, etc. Phenotypic evaluation and genetic characterization of the Camelina and Thlaspi (pennycress) accessions progressed as part of a Ph.D. study. These crops are of interest for biodiesel production. Year three of the GRIN-Global development project was completed. Multiple Release Candidates were distributed for broader testing by international cooperators, and two training sessions were held for international systems administrators and curators. U.S. migration to the new system from GRIN will likely occur in 2012. The Ames and Raleigh Germplasm Enhancement of Maize Projects released 19 lines representing introgression of an additional 12 maize races into elite germplasm. These lines have unique agronomic traits, pest resistance, or value-added grain characteristics and will be used to broaden the genetic diversity of temperate U.S. maize production. About 200 DH lines were developed from previously un-sampled races, but have not yet been released. Financial Situation: Significant accomplishments continue to be achieved at the NCRPIS; financial resources for this project are becoming a major concern. Since 2004, NC-7 funded ISU positions have decreased from 12 to 9. Hatch funding has remained stable at $522,980 but its purchasing power has eroded somewhat over the past decade. Reductions provided technical support for germplasm distribution, field activities, and oilseeds curation. On the USDA-ARS side, funding is approximately the same level as 2003 funding at $2,066,297. Fewer students have been hired (compared to 2003-2005 levels) in order to maintain financial resources for operations, and resources from complementary projects have been leveraged. Regenerations have been reduced for major crops with extensive labor requirements, such as maize, oilseeds, and vegetables. NC-7 Project Participants Research Accomplishments: University of California  Davis researchers have found that plant relatedness (phylogenetic distance) to natives is important for predicting herbivory in sagebrush. Cues that plants use to up-regulate induced responses were studied, and data suggest that these plants rely on airborne volatile cues to communicate, such as low molecular weight terpenes and green leaf volatiles, although the precise nature of these cues is still unknown. Transfer of air containing volatiles emitted by clipped sagebrush plants (captured from their headspace) to non-clipped, non-neighbor plants resulted in less herbivory to the recipients of the transferred air that to recipients of headspace air from non-clipped sagebrush plants. University of Connecticut researchers investigated properties associated with invasive potential of barberry. Barberry is a highly invasive species; the invasive form is associated with green leaf phenotype, while cultivated barberry forms typically have a purple leaf phenotype. Development of sterile clones is necessary for horticultural use that can ensure elimination of invasive risk potential. While at times the purple leaf phenotype may be indistinguishable from the green-leaf phenotype (invasive form), researchers determined that genetically they are distinguishable. University of Delaware researchers have studied the relative results from and relative benefits of using modified single seed descent, doubled haploid, or conventional breeding methods to introgress exotic maize germplasm with elite germplasm. Purdue University, Indiana researcher Jules Janick pursues the history of germplasm utilization through discovery of imagery used in various art forms, among other activities. Completed in 1652, the Taj Mahals floral imagery reflects the extent of floriculture knowledge during the Mughal period. Most of the plants included in the imagery are indigenous to Indian and the Mideast. Iowa State University and/or NCRPIS and ARS researchers are using diverse maize and other grass genetic resources for biomass investigations, development of double haploid lines from exotic germplasm, SNP discovery, and for testing hypotheses regarding the domestication of maize. Extensive investigations of maize kernel starch structure focused on the characterization of amylose-extender (ae) mutant starches and their formation, important for development of food products with specific attribute requirements. Relationships between diverse maize starch properties and ethanol production were also investigated. Wild Helianthus from the desert Southwestern U.S. was incorporated into the collections for potential contribution of drought tolerance traits to cultivated sunflower. Kansas State University researchers continued innovative work on wheat, sorghum, canola, and other crops to enhance basic knowledge on loci and traits associated with productivity, disease and abiotic stress resistance, and to improve crop production. The Brassica napus germplasm collection was evaluated for potential for winter canola variety development for the southern Great Plains with canola cultivars in the final stages of yield testing and are well-adapted to the environmental conditions of the southern Great Plains. Variety Kiowa, derived from a cross between Wichita and PI470056 was released was made available to producers in 2010. Kiowa has higher winter survival over Wichita, a widely grown winter canola cultivar in the U.S. Ten different woody plant accessions are evaluated annually by the John C. Pair Horticultural Center. This program has greatly increased the diversity of woody plants evaluated for suitability to south-central Kansas, and is currently establishing a provenance evaluation of the native Kentucky Coffeetree. Michigan State University researchers completed transcriptional profiling and transcriptomic analysis of cucumber fruit development in order to better understand gene expression related to fruit growth and expansion. Investigations of the gametophytic self-incompatibility (GSI) system in Prunus species determined that the S-RNAse based system exhibits distinct genetic and molecular aspects. In the pollination event, a self-incompatibility (SI) reaction is triggered when the same S allele specificity is expressed in both the pollen and the pistil. Identification of the S determinants led to development of PCR-based S geneotyping, enabling marker assisted selection for self-compatible (SC) individuals. University of Minnesota researchers identified genes differentially expressed between developing seeds of different soybean varieties. University of Missouri researchers and collaborators used plant genetic resources extensively for a very wide range of purposes. Notably, genetic diversity analyses of wild soybean accessions were completed, and an early Group V soybean variety released in 2006 (Stoddard) was registered. University of Nebraska researchers and collaborators compared performance of hard winter wheat cultivar blends with pure cultivars across a wide range of environments. The yield of the cultivar blends was more stable over different environments, with little or no reduction in grain yield; this offers producers an option to limit production risk. Proso millet was genetically characterized, and genomic resources enhanced. The performance of chickpea and dry bean germplasm was evaluated in western Nebraska, and breeding efforts to develop new varieties of these crops with disease resistance progressed. Rutgers University, New Jersey researchers seeking sources of resistance hazelnut germplasm to eastern filbert blight (EFB) inoculated 12 hazelnut genotypes that had exhibited complete resistance in Oregon with pathogen isolates collected from across its native range. EFB causes serious economic losses. An older cultivar, Gasaway, used as a pollinator and currently known to be completely disease free in OR, was reported to be infected at the Rutgers University research farms. Four genotypes were identified that remained free of disease, and others were infected by various isolates or combinations thereof. The Ohio State University researchers evaluated and modified calibration models methods for measurement of tomato carotenoids by infrared spectroscopy, profiled nutritionally important carotenoids from genetically-diverse tomatoes, and their stability in storage (juice product) using infrared spectroscopy. Genetic investigations revealed intron polymorphisms in cultivated tomato via use of both tomato and Arabidopsis genomic information, Grafted and non-grafted tomato transplant production systems in the U.S. were compared and direct and indirect cost sources analyzed. Grafting of herbaceous vegetables is desirable to enable utilization of rootstocks with disease resistance and that support nutrient uptake in combination with scions that offer yield, fruit quality. Use of grafting can reduce economic loss from soilborne diseases with appropriate management strategies, and producers must take economic considerations into account. South Dakota State University researchers and collaborators registered a new spring wheat variety, Brick, with excellent baking and milling qualities, high test weight, early heading, and good Fusarium head blight resistance. Findings from investigations designed to identify dormancy genes from the D genome donor of wheat, potentially useful to improve resistance of cultivars to pre-harvest sprouting, indicate that seeds were more dormant than kernels, suggesting the presence of germination inhibitors in the covereing tissues. Available Aegilops tauschii-derived synthetic hexaploid wheat lines could be an alternative source of dormancy genes for enhancing resistance to pre-harvest sprouting. Texas A&M and Texas Agri-Life researcher reported accomplishments for this period were in collaboration with the institions in Nebraska, Kansas and South Dakota and are already are ported above.

California: Arimura, G., Shiojiri, K. and Karban, R. 2010. Acquired immunity to herbivory and allelopathy caused by airborne plant emissions. Phytochemistry. 71:1642-1649. Heil, M. and Karban, R. 2010. Explaining the evolution of plant communication by airborne signals. Trends in Ecology and Evolution. 25:137-144. Karban, R. 2010. Neighbors affect resistance to herbivory - a new mechanism. New Phytologist. 186:564-566. Karban, R., and Shiojiri, K. 2010. Identity recognition and plant behavior. Plant Signaling and Behavior. 5:854-855. Karban, R., Shiojiri, K. and Ishizaki, S. 2010. An air-transfer experiment confirms the role of volatile cues in communication between plants. American Naturalist. 176:381-384. Connecticut: Lehrer, J. M. and Brand, M.H. 2010. Purple-leaved Japanese barberry (var. atropurpurea) genotypes become visually indistinguishable from green-leaved genotypes (Berberis thunbergii DC.) at low light levels. J. Environmental Horticulture. 28(3):187-189. Indiana: Janick, J. (editor). 2010. Plant Breeding Reviews. 33:386. Janick, J. (editor). 2010. Horticultural Reviews. 36:371. Janick, J. (editor). 2010. Horticultural Reviews. 37:2010. Janick, J. 2010. Plant iconography and art: Source of information on horticultural technology. Bulletin USAVM Horticulture. 67(1):11-23. Janick, J., Kamenetsky, R., and Puttaswamy, S. 2010. Horticulture of the Taj Mahal: Gardens of the imagination. Chronica Horticulturae. 50(3):31-33. Kansas: Djanaguiraman, M., and Prasad, P.V.V. 2010. Ethylene production under high temperature stress causes premature leaf senescence in soybean. Functional Plant Biology. 37:1071-1084. Djanaguiraman, M., Prasad, P.V.V., and Seppanen, M. 2010. Selenium protects sorghum leaves from oxidative damage under high temperature stress by enhancing antioxidant defense system. Plant Physiology and Biochemistry. 48:999-1007. Gholipoor, M., Prasad, P.V.V., Mutava, R.N., and Sinclair, T.R. 2010. Genetic variability of transpiration response to vapor pressure deficit among sorghum genotypes. Field Crops Research. 119:85-90. Kolluru, V., Fritz, A.K.,Paulsen, G.M., Bai, G., Pandravada, S. and Gill, B.S. 2010. Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature. Molecular Breeding. doi:10.1007/s11032-009-9366-8. Stamm, M. 2010. Kiowa Canola. Kans. Ag. Exp. St. and Coop. Ext. Ser., Manhattan, KS. L-928. Michigan: Ando, K., and Grumet, R. 2010. Transcriptional profiling of rapidly growing cucumber fruit by 454-pyrosequencing analysis. J Amer Soc Hortic Sci. 135:291-302. Ando, K., and Grumet, R. 2010. Transcriptomic analysis of cucumber fruit development. Cucurbitaceae 2010 Proceedings. Thies JA, Kousik S, Levi A (Editors). JASHS press. p. 155-158. Bradford, E., Hancock, J.F., and Warner, R.W. 2010. Interactions of temperature and photoperiod determine expression of repeat flowering in strawberry. J. Amer. Soc. Hort. Sci. 135:1-6. Evans, K., Iezzoni, A., Peace, C., Luby, J., Brown, S., van de Weg, E., Main, D., Bassil, N., Fazio, G., Yue, C., Weebadde, C., and McFerson, J. 2010. RosBREED Enabling marker-assisted breeding in the WSU apple breeding program. Journal of Fruit Science. 27 Suppl:43-47. Haldar, S., Haendiges, S., Edge-Garza, D.A.,Oraguzie, N., Olmstead, J.W., Iezzoni, A., and Peace, C. 2010. Applying genetic markers for self-compatibility in the WSU Sweet Cherry Breeding Program. Acta Horticulturae. 859:375-380. Hancock, J.F. Finn, C.E., Luby, J.J., Dale, A., Callow, P.W., and Serce, S. 2010. Reconstruction of the strawberry, Fragaria ´ananassa, using native genotypes of F. virginiana and F. chiloensis. HortScience. 45:1006-1013. Hummer, K.E. and Hancock, J.F. 2010. Strawberry genomics: Botanical history, cultivation, traditional breeding and new technologies. In: Genetics and Genomics of Rosaceae. Springer, New York. Iezzoni, A., Luby, J., Yue, C., McFerson, J., van de Weg, E., Fazio, G., Main, D., Bassil, N., Peace, C., and Weebadde, C. 2010. RosBREED Project Brochure. Iezzoni, A., Luby, J., Yue, C., van de Weg, E., Fazio, G., Main, D., Bassil, N., Weebadde, C., McFerson, J., and Peace, C. 2010. RosBREED: Enabling marker-assisted breeding in Rosaceae. Acta Horticulturae. 859:389-394. Olmstead, J.W., Whiting, M.D., Lang, G., Ophardt, D., and Oraguzie, N. 2010. PC7146-8 (Benton) Sweet Cherry. HortScience. 46(1):121-122. Olmstead, J.W., Whiting, M.D., Lang, G., Ophardt, D., and Oraguzie, N. 2010. PC7064(Selah) Sweet Cherry. HortScience. 46(1):123-124. Oraguzie, N., Ophardt, D., Long, L., Lang, G., and Whiting, M.D. 2010. KionaTM Sweet Cherry. HortScience. 45(12):1906-1907. Peace, C., Fazio, F., Evans, K., Weebadde, C., and Iezzoni, A. 2010. Marker-Assisted Breeding (MAB) Pipeline Team goals and activities: Channeling socio-economic and DNA information into routine breeding operations. RosBREED Project Brochure. Siddiq, M., Iezzoni, A., Khan, A., Breen, P., Sebolt, A., Dolan, K., and Ravi, R. 2010. Characterization of new tart cherry (Prunus cerasus L.) selections based on fruit quality, total anthocyanins, and antioxidant capacity. Intl. J. Food Properties. doi: 10.1080/10942910903277697. Song, G.Q., Loescher, W.H., Sink, K.C., Ma, Y., Herlache, T., and Hancock, J.F. 2010. A novel mannose-based selection system for plant transformation using celery mannose-6-phosphate reductase gene (M6PR). Plant Cell Reports. 29:163-172. Stegmeir, T.L., Finn, C.E., Warner, R., and Hancock, J.F. 2010. Performance of an elite strawberry population derived from wild germplasm of Fragaria chiloensis and F. virginiana. HortScience. 45:1140-1145. Tao, R., and Iezzoni, A.F. 2010. The S-RNase-based gametophytic self-incompatibility system in Prunus exhibits distinct genetic and molecular features. Scientia Horticulturae. 124:423-433. Tsukamoto, T., Hauck, N.R., Tao, R., Jiang, J., and Iezzoni, A.F. 2010. Molecular and genetic analysis of four nonfunctional S haplotype variants derived from a common ancestral S haplotype identified in sour cherry (Prunus cerasus L.). Genetics. 184:411-427. Weebadde, C., Sebolt, A., Iezzoni, A., and Peace, C. 2010. RosBREED: Enabling marker-assisted breeding. In: Rosaceae Newsletter. 1(4). Minnesota: Lin, R., Glazebrook, J., Katugari, F., Orf, J.H., and Gibson, S.L. 2010. Identification of genes differentially expressed between developing seeds of different soybean varieties. BMC Plant Biology. 10:278. Non-Peer Reviewed: Bolen, Y-T., Haun, W., Muehlbauer, G., Orf, J., Naeve, S., Stupar, R., and Vance, C. 2010. Fast neutron mutagenesis of soybean: A resource for the community. 13th Biennial Molecular and Cellular Biology of the Soybean Conference 2010. Chen, S., Potter, B., and Orf, J.H. 2010. Virulence of the soybean cyst nematode has increased over years in Minnesota. Proc. Soc. of Nematologists. Naeve, S.L., Orf, J.H., and Miller-Garvin, J. 2010. Analysis of the U.S. Non-GMO Food Soybean Variety Pipeline. Proc. Second Soyfood Alliance Conference, Tokyo, Japan. 07/01/2010. Naeve, S.L., Orf, J.H., Miller-Garvin, J., and O'Neill, T. 2010. Quality of the United States Food Soybean Crop 2010. USSEC Conferences Korea and Japan. Naeve, S.L., Orf, J.H., and Miller-Garvin, J. 2010. Quality of the United States soybean crop 2010. USSEC Conferences China, Japan, Korea, and Taiwan. Orf, J.H. 2010. Soybean Breeding in the Northern U.S. Proc. 6th Annual National Center for Soybean Biotechnology Symposium. Orf, J.H., Naeve, S.L., Schaus, P.J., and Killam, A. 2010. Minnesota Certified Seed Guide 2010. p. 50-69. Orf, J.H., Naeve, S.L., Schaus, P.J., and Killam, A. 2010. MP116-2010. p. 48-68. Missouri: Shannon, J.G., Nelson, R.L., and Wrather, J.A. 2010. Registration of LG04-6863 soybean germplasm line with diverse pedigree. J. of Plant Registrations. 4:70-72. Vuong, T., Sleper, D., Shannon, J., and Nguyen, H. 2010. Novel quantitative trait loci for broad-based resistance to soybean cyst nematode (Heterodera glycines Ichinohe) in soybean PI 567516C. TAG. doi: 10.1007/s00122-010-1385-7. Nebraska: Glover, K.D., Rudd, J.C., Devkota, R.N., Hall, R.G., Jin, Y., Osborne, L.E., Ingemansen, J.A., Rickertsen, J.R., Baltensperger, D.D., and Hareland, G.A. 2010. Registration of "Brick" Wheat. Journal of Plant Registrations. 4(1):22-27. Mengistu, N., Baenziger, P.S., Nelson, L.A., Eskridge, K.M., Klein, R.N., Baltensperger, D.D., and Elmore, R.W. 2010. Grain Yield Performance and Stability of Cultivar Blends vs. Component Cultivars of Hard Winter Wheat in Nebraska. Crop Science. 50(March-April). Pavlista, A.D., Isbell, T.A., Baltensperger, D.D., and Hergert, C.W. 2010. Planting date and development of spring-seeded irrigated canola, brown mustard and camelina. Industrial Crops and Products. 33:451-456 Santra D. K., Plyler-Harveson, R., Harvey, S., Reddy, S., and Frickel, G. 2010. Characterization of proso millet (Panicum miliaceum L.) germplasm. Isbell, T.A. and Dierig, D.A. Eds. (2010). In: 22nd Annual AAIC Meeting 2010 New Crops: Exploring Diversity and Preserving Our Future: Program and Abstracts. Hilton Hotel, Fort Collins, CO. p. 48. Santra D. K., Plyler-Harveson, R., Harvey, S., Reddy, S., and Frickel, G. 2010. Genetic characterization of proso millet (Panicum miliaceum L.) germplasm. Proceedings of ASA-CSSA-SSSA 2010 International Annual Meeting. Long Beach, CA. 10/31-11/04/2010. p. 118. Urrea, C.A., Harveson, R.M., Koehler, A.E., Burgener, P., and Baltensperger, D.D. 2010. Evaluating the Agronomic Potential of Chickpea Germplasm for Western Nebraska. Agronomy Journal. 102(4):1179-1185. New Jersey: Molnar, T.J., Capik, J., Zhao, S., and Zhang, N. 2010. First report of Eastern Filbert Blight on Corylus avellana Gasaway and VR20-11 caused by Anisogramma anomala (Peck) E. Muller in New Jersey. Plant Disease. 94:1265. Molnar, T.J., Goffreda, J.C., and Funk, C.R. 2010. Survey of Corylus Resistance to Anisogramma anomala from Different Geographic Locations. HortScience. 45:832-836. Ohio: Gupta, R., Balasubramaniam, V.M., Schwartz, S.J., and Francis, D.M. 2010. Storage stability of lycopene in tomato juice subjected to combined pressure-heat treatments. J Agric Food Chem. 58(14):8305-8313. Hutton S.F., Scott J.W., Yang W., Sim S.C., Francis D.M., and Jones J.B. 2010. Identification of QTL associated with resistance to bacterial spot race T4 in tomato. Theor Appl Genet. 121(7):1275-1287 [Epub ahead of print]. http://www.springerlink.com/content/04p1205n44176p40/. Robbins, M.D., Masud, M.A.T., Panthee, D.P., Gardner, R.G., Francis, D.M., and Stevens, M.R. 2010. Marker-assisted Selection for Coupling Phase Resistance to Tomato spotted wilt virus and Phytophthora infestans (Late Blight) in Tomato. Hortscience. 45(10):1424-1428. Robbins, M.D., Sim, S-C., Yang, W., Van Deynze, A., van der Knaap, E., Joobeur, T., and Francis, D.M. 2010. Mapping and linkage disequilibrium analysis with a genome-wide collection of SNPs that detect polymorphism in cultivated tomato. Journal of Experimental Botany. http://jxb.oxfordjournals.org/content/early/2010/12/30/jxb.erq367. Rodriguez, G.R., Moyseenko, J.B., Robbins, M.D., Huarachi Morejon, N., Francis, D.M., and van der Knaap, E. 2010. Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects. J. Vis. Exp. (JoVE). 37. http://www.jove.com/index/details.stpid=1856, doi: 10.3791/1856. Rubio-Diaz, D.E., Santos, A., Francis, D.M., and Rodriguez-Saona, L.E. 2010. Carotenoid stability during production and storage of tomato juice made from tomatoes with diverse pigment profiles measured by infrared spectroscopy. J Agric Food Chem. 58(15):8692-8698. Rubio-Diaz, D.E., De Nardo, T., Santos, A., de Jesus, S., Francis, D., and Rodriguez-Saona, L.E. 2009. Profiling of nutritionally important carotenoids from genetically-diverse tomatoes by infrared spectroscopy. Food Chem. 120:282-289. Rubio-Diaz, D.E, Francis, D.M., and Rodriguez-Saona, L.E. 2010. External calibration models for the measurement of tomato carotenoids by infrared spectroscopy. Journal of Food Composition and Analysis 24(1):121-126. doi: 10.1016/j.jfca.2010.06.006. Rubio-Diaz, D.E., Santos, A., Francis, D.M., and Rodriguez-Saona, L.E. 2010. Carotenoid Stability during Production and Storage of Tomato Juice Made from Tomatoes with Diverse Pigment Profiles Measured by Infrared Spectroscopy. J. Agric. Food Chem. 58, 8692-8698 Rivard, C.L., Sydorovych, O., OConnell, S., Peet, M.M., and Louws, F.L. 2010. An Economic Analysis of Two Grafted Tomato Transplant Production Systems in the United States. Hort. Technology. 20:794-803. Sammons, J. D. and Struve, D. K. 2010. The effects of near-zero leachate irrigation on growth and water use efficiency and nutrient uptake of container grown baldcypress (Taxodium distichum (L.) Rich.) plants. J. Environ. Hort. 28:27-34. Struve, D., Ferrini, F., Chandra, B., and Fini, A. 2010. Propagation of Quercus cerris, Q. petraea, and Q/ pubescens seedlings by stem cuttings. HortScience. 45:172901733. Sim, S-C., Robbins, M.D., Van Deynze, A., Michel, A.P., and Francis, D.M. 2010. Population structure and genetic differentiation associated with breeding history and selection in tomato (Solanum lycopersicum L.). Heredity 106:927-935. doi:10.1038/hdy.2010.139 Wang, Y., Chen, J., Francis, D.M., Shen, H., Wu, T., and Yang, W. 2010. Discovery of intron polymorphisms in cultivated tomato using both tomato and Arabidopsis genomic information. Theor Appl Genet. 121(7):1199-1207. http://www.springerlink.com/content/t67t760578v35130/. Not Peer-Reviewed: Francis, D., Schealeppi, J., Pisarski , V. and Huarachi, N. 2010. Grafting with Glue, 06/24/2010. Youtube: http://www.youtube.co/watchv=5Fd6tBQTTAg . South Dakota: Caffe-Treml, M., Glover, K.D., Krishnan, P., and Hareland, G.A. 2010. Variability and Relationships Among Mixolab, Mixograph, and Baking Parameters Based on Multi-Environment Spring Wheat Trials. Cereal Chemistry. 67:574-580. Glover K. D., Rudd, J. C., Devkota, R. N., Hall, R.G., Jin, Y., Osborne, L.E., Ingemansen, J.A., Rickertsen, J.R., Baltensperger, D. D. and Hareland, G. A. 2010. Registration of Brick Wheat. Journal of Plant Registrations. 4:22-27. Gu, X.-Y., Zhang, L., Glover, K.D., Chu, C., Xu, S.S., Faris, J.D., Friesen, T.L., and Ibrahim, A. 2010. Genetic variation of seed dormancy in synthetic hexaploid wheat-derived populations. Crop Science. 50:1318-1324. Malla, S., Ibrahim, A.M.H., Glover, K.D., and Berzonsky, W.A. 2010. Combining Ability of Fusarium head blight resistance in wheat. Commun. Biometry Crop Sci. 5:116-126. Malla, S., Ibrahim, A.M.H., Little, R., Kalsbeck, S., Glover, K.D., Ren, C. 2010. Comparison of shifted multiplicative model, rank correlation, and biplot analysis for clustering winter wheat production environments. Euphytica. 174:157-170. Malla, S., Ibrahim, A.M.H., Yen, Y., Berzonsky, W., Glover, K.D., and Stein, J. 2010. Analysis of a Putative Novel Source of Resistance to Fusarium Head Blight in Hard Winter Wheat. International Journal of Plant Breeding. 4:47-54. Malla, S., Ibrahim, A.M.H., Yen, Y., Berzonsky, W., Glover, K.D., and Stein, J. 2010. Quantitative Trait Loci Analysis of Novel Fusarium Head Blight Resistance in Tokai 66. Am. J. of Ag. Bio. Sci. 5:62-69. Mergoum, M., Glover, K.D., Anderson, J.A., Gigax, D., Berg, J., Singh, P.K., Ransom, J.K., and Isakson, P.J. 2010. Development and Agronomic Performance of Transgenic Roundup Ready Spring Wheat in the North Central Plains of the United States. Agronomy Journal. 102:1462-1467 NCRPIS: Jiang, H., Campbell, M., Blanco, M., and Jane, J. 2010. Characterization of maize amylose-extender (ae) mutant starches: Part II. Structures and properties of starch residues remaining after enzymatic hydrolysis at boiling-water temperature. Carbohydrate Polymers. 80(1):1-12. Jiang, H., Horner, H., Pepper, T., Blanco, M., Campbell, M., and J. Jane. 2010. Formation of elongated starch granules in high-amylose maize. Carbohydrate Polymers. 80(2):534-539. Kovach, D.A., Widrlechner, M.P., and Brenner, D.M. 2010. Variation in seed dormancy in Echinochloa and the development of a standard protocol for germination testing. Seed Sci. and Technol. 38:559-571. Qu, L., Widrlechner, M.P., and Rigby, SM. 2010. Analysis of breeding systems, ploidy, and the role of hexaploids in three Hypericum perforatum L. populations. Industrial Crops and Products. 32:1-6. Srichuwong, S., Gutsea, J., Blanco, M.H., Duvick, S.A., Gardner, C.A., and Jane, J. 2010. Characterization of Corn Grains for Ethanol Production. Journal of ASTM International. 7(2):1-10. NCRPIS Non-Peer Reviewed: Ayala-Diaz, I.M., Gardner, C.A., Isbell, T., Marek, L., and Westgate, M. 2010. Effect of temperature gradients on germination of pennycress germplasm. (abstract) 2010 Association for the Advancement of Industrial Crops Annual Meeting, 09/18-22/2010, Ft. Collins, CO. Ayala-Diaz, I.M., Gardner, C.A., Isbell, T., Marek, L., and Westgate, M. 2010. Variations in fatty acid composition and oil content in camelina germplasm. (abstract) 2010 ASA-CSSA-SSSA International Annual Meeting, Longbeach, California, 10/30-11/04/2010. Cyr, P.D. , Weaver, B.E. , Millard, M.J., Gardner, C.A., Bohning, M.A., Emberland, G., Sinnott, Q.P., Kinard, G.P., Franco, T., Mackay, M., Guarino, L., and Bretting, P.K. 2010. GRIN-Global: An international project to develop a global plant genebank and information management system. (Abstract P800). Plant and Animal Genome XVII Conference Abstracts, San Diego, CA, 01/10-14/2010. Gulya, T., Marek, L.F., and Gavrilova, V. 2010. Disease resistance in cultivated sunflower derived from public germplasm collections. Plenary talk/Proceedings of the International Symposium Sunflower Breeding on Resistance to Diseases, Krasnodar, Russia, June 23-24, 2010, sponsored by the All-Russia Research Institute of Oil Crops (VNIIMK) and the International Sunflower Association. Marek, L.F. 2010. Plant Exploration Final Report: 2009 Plant Exploration in the South Central United States to collect Sunflower Germplasm for Crop Improvement. Plant Exchange Office, National Germplasm Resources Laboratory, USDA-ARS, Beltsville, MD. Medic, J., Abendroth, L., Elmore, R., Blanco, M.H., and Jane, J. 2010. Effect of planting date on cornstarch structures and properties. (abstract) American Assocation of Cereal Chemists Annual Meeting, Savannah, GA, O10/24-27/2010. Sandhu, S., Barb, J., Gracom, K. Sykes, R., Moyers, B., Davis, M., Rieseberg, L., Marek, L., Burke, J., and Knapp, S. 2010. Genomic analysis of wood production in sunflower. (abstract 208-2). 2010 ASA-CSSA-SSSA Annual Meeting, Long Beach, CA, 10/31-11/04/2010. Smelser, A.D., Brenner, E., Vanous, A., Blanco, M.H., Lubberstedt, T., Frei, U., and Gardner, C.A. 2010. Maize haploid induction and doubling, recent experience with exotic and elite maize populations. (abstract 89-10). 2010 ASA-CSSA-SSSA International Annual Meeting, Longbeach, CA. 10/30-11/04/2010. Widrlechner, M.P. 2010. Building a comprehensive collection of ash germplasm. (abstract) 4th Global Botanic Gardens Congress, Dublin, Ireland, 05/13-18/2010. Conference Program and Book of Abstracts. P. 114-115.