Brief Summary of Minutes of Annual Meeting

Members of the NC1004 Multistate Project (Genetic and Functional Genomic Approaches to Improve Production and Quality of Pork) met on December 10-11, 2004 in conjunction with the National Swine Improvement Federation Annual Meeting in Ames, IA. This was the third meeting of the committee and covered the period January 11 through December 10, 2004. Transcriptional profiling of specific tissues has been a major emphasis of member stations and several stations have utilized the 13,000 70-mer oligonucleotide microarray developed with support from NRSP8 swine genome coordination funds. The Iowa station has used this microarray to identify tissue-specific expression patterns in adult pig lung, liver, small intestine and skeletal muscle tissues. The Michigan station has used this array to evaluate expression patterns in skeletal muscle tissue during secondary fiber formation in mid-gestation and in early postnatal growth. The Iowa station has also used this array along with the technique of subtractive suppression hybridization to identify genes responding to Salmonella infection in mesenteric lymph nodes and lung tissue. The USDA-BARC station has used gene expression assays to assess responses to vaccination for porcine reproductive and respiratory syndrome virus (PRRSV) and also to characterize the immune factors which regulate interferon-gamma dominated immune responses in order to help identify populations of pigs that are susceptible or resistant to respiratory infections. In addition, the Iowa and Oklahoma stations have initiated a project to evaluate gene expression patterns in endometrium and embryo/conceptus during the elongation and implantation phase of reproduction in the Yorkshire and Meishan breeds of pigs. Efforts have continued at several stations to pursue detection of quantitative trait loci (QTL), with efforts at Michigan and Iowa focused primarily on meat quality traits. The Michigan station completed data collection on an F2 Duroc/Pietrain resource population with a total of 958 F2 progeny having complete phenotypic records, and microsatellite genotyping of this population is currently underway. The Iowa station has begun to implement discriminant analysis methods for multitrait QTL detection. The Iowa station also completed a joint QTL analysis of the Iowa State University Berkshire/Yorkshire resource population and the University of Illinois Berkshire/Duroc resource population. The Nebraska station is pursuing fine mapping of QTL for reproductive traits in the NE selection lines. They have used pigs with phenotypic records from several generations of the selection project, and have genotyped these animals using a panel of single nucleotide polymorphism (SNP) markers for four putative QTL regions. The Iowa station has also continued to develop marker-assisted selection strategies, with recent emphasis on efficiency of selection for multiple QTL for a trait in a crossbred population. Evaluation of candidate genes for various traits remains a major emphasis of several stations. The Iowa station has studied several genes affecting meat quality and has also begun to target genes associated with sow longevity. The North Carolina station has begun to characterize follistatin as a candidate gene for litter size. The Vermont station is studying the biological functions of the porcine prolactin receptor in reproduction and lactation. The Oklahoma station has mapped the porcine Kallikreine gene family and has evaluated the expression patterns for several of these genes during early embryonic development in both endometrial and conceptus tissues. Development of selection lines and evaluation of unique genetic resources continued at several member stations. The Nebraska station continued a project begun in 1981 to select for litter size and its component traits, which involved development of five lines. These lines have now been crossed in order to maintain the high reproductive rate, preserve the integrity of the index selection line and reduce inbreeding levels. The North Carolina station has two Duroc selection lines differing in endogenous testosterone production, and purebred and crossbred progeny from these lines have been evaluated for growth and carcass composition traits. The Ohio station has initiated a selection experiment for improved pork quality using the Landrace breed. The Iowa station has continued selection projects for residual feed intake and intramuscular fat deposition. The Iowa station has used frozen semen to produce offspring from Duroc sires used in the 1980s and current Duroc sires in order to evaluate the impact of selection for leanness on carcass and meat quality traits. The Iowa station has studied factors influencing sow longevity and determined that selection for leg confirmation is beneficial for improving longevity. The Iowa station has also studied the relationship between myoglobin content and longissimus muscle color. Finally, the North Carolina station in collaboration with the USDA-MARC station has estimated variance components due to genetic competition effects in pigs. In summary, significant progress has been made toward accomplishing the outcomes and outputs defined for the NC1004 multistate project. Numerous collaborative linkages have developed between the NC1004 member stations (examples include IA/IL, IA/BARC/OK, MI/OK, NE/BARC, NC/MARC, VT/MARC) and many of these partnerships are resulting in successful grant funding, which is allowing research efforts for the project to move forward. Shared resources and public availability of data have been a cornerstone of the project and these continue to be developed and distributed. Integration of quantitative and molecular information facilitates rapid discovery of genetic variation influencing pig health, production efficiency and pork quality. In addition, outreach activities and interaction with industry scientists and producers allow transfer of knowledge to the industry.

* Indicates joint publications of stations within NC1004 project IOWA *Baas, T. J., K. J. Stalder, J. W. Mabry, and M. T. See. 2004. Effect of off test backfat, loin muscle area, and days to 114 kg on the genetic merit of Yorkshire females for reproductive traits. 15th International Congress on Animal Reproduction, Porto Seguro, Brazil. *Cassady, J. P., O. W. Robison, R. K. Johnson, J. W. Mabry, L. L. Christian, M. D. Tokach, R. K. Miller, and R. N. Goodwin. 2004. National Pork Producers Council Maternal Line Genetic Evaluation: A comparison of growth and carcass traits in terminal progeny. J. Anim. Sci. 82:3482-3485. Ciobanu, D.C., J.W.M. Bastiaansen, S.M. Lonergan, H. Thomsen, J.C.M. Dekkers, G.S. Plastow, and M.F. Rothschild. 2004. New alleles in calpastatin gene are associated with meat quality traits in pigs. J. Animal Sci. 82:2829-2839. Ciobanu, D.C., Lonergan, S.M., Bastiaansen, J.W.M.,Mileham, Miculinich,, Schultz-Kaster, C., Sosnicki, A.A., Plastow , G.S. and M.F. Rothschild. 2004. Association of new Calpastatin alleles with meat quality traits in commercial pigs. 50th Int. Congress of Meat Science and Technology, Helsinki, Finland. Dekkers, J.C.M. 2004. Commercial application of marker- and gene-assisted selection in livestock: strategies and lessons. J. Anim. Sci. 82: E313-328E Dekkers, J.C.M., and R. Chakraborty. 2004. Optimizing purebred selection for crossbred performance using QTL. Genet. Sel. Evol. 36: 297-324. Fernando, R.L., B. R. Southey, J.C.M. Dekkers, M.F. Rothschild, and M. Soller. 2004. Controlling the proportion of false positives (PFP) in a multiple test situation. Genetics 166: 611-619. Gaboreanu, A.M., L. Grapes, A. M. Ramos, J.-J. Kim and M. F. Rothschild. 2004. Characterization of an X-chromosome PCR-RFLP marker associated with fat deposition and growth in the pig. Animal Genetics 35: 401-403. Grapes, L., J.C.M. Dekkers, M.F. Rothschild, and R.L. Fernando. 2004. Comparing linkage disequilibrium-based methods for fine mapping quantitative trait loci. Genetics 166: 1561-1570. Grapes, L., M. Z. Firat, J. C. M. Dekkers, M. F. Rothschild, and R. L. Fernando. 2004. Optimal haplotype structure for linkage disequilibrium-based fine mapping of quantitative trait loci. American Association of Animal Science Midwest Region, Mar 15-17, Des Moines, IA Grapes, L., S. Rudd, R. L. Fernando, and M. F. Rothschild. 2004. In silico SNP identification from porcine EST sequences and comparative analysis with human SNP density. Plant and Animal Genome XII, Jan 10-14, San Diego, CA Grindflek, E, N Hoen, H Sundvold, MF Rothschild, G Plastow, and S Lien. 2004. Investigation of a Peroxisome Proliferator Activated Receptor gamma (PPARG) haplotype effect on meat quality and carcass traits in pigs. Anim. Genet 35:238-241. Houser, T. A., J. G. Sebranek, B. J. Thacker, T. J. Baas, D. Nilubol, E. L. Thacker, and F. Kruse. 2004. Effectiveness of transdermal, needle-free injections for reducing pork carcass defects. Meat Science 68:329-332. Hu, Z-L, K. Glenn, A. M. Ramos, C. J. Otieno, and M. F. Rothschild. 2004. Expeditor: A Pipeline for Designing Pig Primers Using Human Gene Structure and Pig EST Information. Plant and Animal Genome XII, Jan 10-14, San Diego, CA *Johnson, R. K., E. P. Berg, R. Goodwin, J. W. Mabry, R. K. Miller, O. W. Robison, H. Sellers and M. D. Tokach. 2004. Evaluation of procedures to predict fat-free lean in swine carcasses. J. Anim. Sci. 82:2428-2441. Kim, J.-J. and J. C. M. Dekkers. 2004. A combined line-cross and halfsib model to detect and characterize QTL in an F2 outbred cross population. American Society of Animal Science Annual meeting (Abstract). http://www.fass.org/2004/abstracts/414.PDF Kim, K.S., J. J. Kim, J. C. M. Dekkers, and M. F. Rothschild. 2004. Polar overdominant inheritance of a DLK1 polymorphism is associated with growth and fatness in pigs. Mammalian Genome 15:552-559. Kim, K.S., J.J. Kim, J.C.M. Dekkers, and M.F. Rothschild. 2004. Polar overdominance imprinting is associated with growth and fat deposition in pigs. PAG XII p240 Kim, K.S., J.M. Reecy, W.H. Hsu, and L.L. Anderson. 2004. Functional and phylogenetic analyses of a melanocortin-4 receptor mutation in domestic pigs. Domestic Animal Endocrinology 26: 75-86. Martin, B. D., T. J. Baas, C. Schwab, D. W. Newcom, J. F. Lampe, and K. J. Stalder. 2004. Comparison of deposition rates for loin muscle area, backfat, and intramuscular fat percentage among breeds in the 2003 National Barrow Show Sire Progeny Test. J. Anim. Sci. 82 (Suppl. 1):455. *Moeller, S. J., R. N. Goodwin, R. K. Johnson, J. W. Mabry, T. J. Baas, and O. W. Robison. 2004. The National Pork Producers Council Maternal Line National Genetic Evaluation Program: A comparison of six maternal genetic lines for female productivity measures over four parities. J. Anim Sci. 82:41-53. *Moller, M., F. Berg, J. Riquet, D. Pomp, A. Archibald, S. Anderson, K. Feve, Y. Zhang, M.F. Rothschild, D. Milan, L. Andersson and C.K. Tuggle. 2004. High-resolution comparative mapping across pig chromosome 4 (SSC4), emphasizing the FAT1 region. Mammalian Genome 15: 717-31. Mote, B. E., J. D. Loy, and M. F. Rothschild. 2004. Identification of SNPs in the insulin-like growth factor gene family and subsequent mapping of IGF2R and IGFBP1 in pigs. Plant and Animal Genome XII, Jan 10-14, San Diego, CA Newcom, D. W., J. L. Burkett, T. J. Baas, C. R. Schwab, S. J. Moeller, and K. J. Stalder. 2004. Effect of technician, machine, and animal body composition on accuracy of ultrasonic measures of backfat and loin muscle area in swine. J. Anim. Sci. 82 (Suppl. 2):46. Newcom, D. W., K. J. Stalder, T. J. Baas, R. N. Goodwin, F. C. Parrish, and B. R. Wiegand. 2004. Breed differences and genetic parameters of myoglobin concentration in porcine longissimus muscle. J. Anim Sci. 82:2264-2268. Newcom, D. W., T. J. Baas, and K. J. Stalder. 2004. Relationship between backfat depth and its individual layers and intramuscular fat percentage in swine. J. Anim. Sci. 82 (Suppl. 2):39. Rothschild, M.F. 2004. Porcine genomics delivers new tools and results: This little piggy did more than just go to market. Genetical Research 83:1-6. Rothschild, M.F. 2004. DNA advances offer big payoffs. Pig Progress Magazine. 20:1-3. Rothschild, M.F. J. P. Bidanel and D.C. Ciobanu. 2004. Genome Analysis of QTL for Muscle Tissue Development and Meat Quality. In: Muscle Development of Livestock Animals. Physiology, Genetics and Muscle Quality. Eds: M.F. W. te Pas, H.P. Haagsman and M.E. Everts. CABI Publishing pgs 247-266. Rothschild, M.F., G.S. Plastow and S. Newman. 2004. Patenting in animal breeding and genetics. In: WAAP Book of the Year 2003, Eds: A. Rosati, A. Tewolde and C. Mosconi. Pgs 269-280. Schwab, C. R., T. J. Baas, D. W. Newcom, and K. J. Stalder. 2004. An evaluation of performance and carcass characteristics between pigs sired by boars from two different time periods. J. Anim. Sci. 82 (Suppl. 1): 453. Schwab, C. R., T. J. Baas, D. W. Newcom, and K. J. Stalder. 2004. An evaluation of meat and eating quality traits between pigs sired by boars from two different time periods. J. Anim. Sci. 82 (Suppl. 1): 454. Schwab, C. R., T. J. Baas, D. W. Newcom, and K. J. Stalder. 2004. Comparison of growth patterns for loin muscle area, backfat, and intramuscular fat percentage between pigs sired by boars from two different time periods. J. Anim. Sci. 82 (Suppl. 2): 9. Serenius, T., and K. J. Stalder. 2004. Genetics of length of productive life and lifetime prolificacy in the Finnish Landrace and Large White pig populations. J. Anim. Sci. 82:3111-3117. Stalder, K. J., M. Knauer, T. J. Baas, M. F. Rothschild, and J. W. Mabry. 2004. Sow Longevity. Pig News and Information. 25:53N-74N. Thomsen, H., J. C. M. Dekkers, H. K. Lee, and M. F. Rothschild. 2004. Characterization of quantitative trait loci for growth and meat quality in a cross between commercial breeds of swine J. Anim. Sci. 82:2213-2228. Totir, L.R., R.L. Fernando, J.C.M. Dekkers, and S.A. Fernandez. 2004. A study of the minimum number of loci required for genetic evaluation using a finite locus model. Genet. Sel. Evol. 36: 395-414 Totir, L.R., R.L. Fernando, J.C.M. Dekkers, S.A. Fernandez, and B. Guldbrandtsen. 2004. Effect of using approximate gametic variance covariance matrices on marker assisted selection by BLUP. Genet. Sel. Evol. 36:29-48. Tuggle, C.K., and Midwest Consortium. 2004. Finding the genes expressed in female reproductive tissues in pigs. Bulletin AS 650, January, 2004, Animal Industry Report 2004, Iowa State University, Ames, IA ASL-R1950. Tuggle, C.K., X.W. Shi, L. Marklund, A. Stumbaugh, T.J. Stabel, M.A Mellencamp, L. Galina-Pantoja, and J. Bastiaansen 2004. Association of bacterial infection traits with genetic variation at candidate genes for porcine disease resistance. Bulletin AS 650, Animal Industry Report 2004, Iowa State University, Ames, IA ASL-R1952. *Tuggle, C.K., Y. Zhang, M.F. Rothschild, M.Moller, F. Berg, L. Anderson, J. Riquet, D. Milan, D. Pomp, A. Archibald, and S. Anderson. 2004. A detailed gene map of pig chromosome 4, where the first quantitative trait locus in livestock was mapped. Bulletin AS 650, Animal Industry Report 2004, Iowa State University, Ames, IA ASL-R1951. Villanueva, B., J.C.M. Dekkers, J. A. Woolliams, and P. Settar. 2004. Maximizing genetic gain over multiple generations with quantitative trait locus selection and control of inbreeding. J. Anim Sci. 82: 1305-1314. Wilke, V. L., B. P. Kinghorn, M. G. Conzemius, and M. F. Rothschild. 2004. Prediction of inheritance for cranial cruciate ligament disease in the Newfoundland dog. Abstract to be presented at the 31st Annual Conference of the Veterinary Orthopedic Society. Wilke, V. L., B. P. Kinghorn, M. G. Conzemius, and M. F. Rothschild. 2004. Prediction of inheritance for cranial cruciate ligament disease in the Newfoundland dog. Presented at the 2004 ASAS/ADSA Midwestern Meeting, Des Moines, IA. Zhao, H., D. Nettleton, M. Soller, and J. C. M. Dekkers. 2004. Linkage disequilibrium measures between markers as predictors of linkage disequilibrium between markers and QTL. J. Anim Sci. 82 (Suppl. 2) p.9. Zhao, H., J.-J. Kim , M. Perez-Enciso, and J. C. M. Dekkers. 2004. Detection of quantitative trait loci segregation within pure breeds in a Berkshire x Yorkshire F2 population.American Society of Animal Science Annual meeting (Abstract). http://www.fass.org/2004/abstracts/452.PDF Zhao, S.-H. A. Erickson, and C.K. Tuggle. 2004. Physical and Linkage mapping of lymphocyte antigen 86 (Ly86) gene to porcine chromosome 7. Anim. Genet. 35:164. Zhao, S.-H. and C.K. Tuggle. 2004. Linkage mapping and expression analyses during early gestation in the pig of a novel gene, PLacentally Expressed Transcript 1 (PLET1). Anim. Genet. 35:72-74. Zhao, S.-H., D. G. Simmons, J.C. Cross, T.E. Scheetz, T.L. Casavant, M. B. Soares, and C.K. Tuggle. 2004. PLET1, a highly expressed and processed novel gene in pig and mouse placenta is transcribed but poorly spliced in human. Genomics 84:114-125. MICHIGAN Ernst, C.W., N.E. Raney, V.D. Rilington, G.A. Rohrer, J.A. Brouillette and P.J. Venta. 2004. Mapping of the FES and FURIN genes to porcine chromosome 7. Anim. Genet. 35:142-143. Farber, C.R., N.E. Raney, V.D. Rilington, P.J. Venta and C.W. Ernst. 2003. Comparative mapping of genes flanking the human chromosome 12 evolutionary breakpoint in the pig. Cytogenet. Genome Res. 102:139-144. Wesolowski, S.R., N.E. Raney and C.W. Ernst. 2004. Developmental changes in the fetal pig transcriptome. Physiol. Genomics. 16:268-274. NEBRASKA *T. J. Baas, R. N. Goodwin, L. L. Christian, R. K. Johnson, O. W. Robison, J. W. Mabry, K. Clark, M. Tokach, S. Henry and P. J. Berger. 2003. Design and standards for genetic evaluation of swine seedstock populations . J. Anim. Sci. 2003. 81:2409-2418. *J. P. Cassady, O. W. Robison, R. K. Johnson, J. W. Mabry, L. L. Christian, M. D. Tokach, R. K. Miller and R. N. Goodwin. 2004. National Pork Producers Council Maternal Line Genetic Evaluation: A comparison of growth and carcass traits in terminal progeny. J. Anim. Sci. 2004. 82:3482-3485. *J. W. Holl, J. P. Cassady, D. Pomp and R. K. Johnson. 2004. A genome scan for quantitative trait loci and imprinted regions affecting reproduction in pigs. J. Anim. Sci. 2004. 82:3421-3429. *R. K. Johnson, E. P. Berg, R. Goodwin, J. W. Mabry, R. K. Miller, O. W. Robison, H. Sellers and M. D. Tokach. 2004. Evaluation of procedures to predict fat-free lean in swine carcasses. J. Anim. Sci. 2004. 82:2428-2441. D. B. Petry and R. K. Johnson. 2004. Responses to 19 generations of litter size selection in the Nebraska Index line. I. Reproductive responses estimated in pure line and crossbred litters. J. Anim. Sci. 2004. 82:1000-1006. D. B. Petry, J. W. Holl and R. K. Johnson. 2004. Responses to 19 generations of litter size selection in the NE Index line. II. Growth and carcass responses estimated in pure line and crossbred litters. J. Anim. Sci. 2004. 82:1895-1902. *S. J. Moeller, R. N. Goodwin, R. K. Johnson, J. W. Mabry, T. J. Baas and O. W. Robison. 2004. The National Pork Producers Council Maternal Line National Genetic Evaluation Program: A comparison of six maternal genetic lines for female productivity measures over four parities. J. Anim. Sci. 2004. 82:41-53. G. R. Bertani, C. D. Gladney, R. K. Johnson and D. Pomp. 2004. Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: II. Anterior pituitary. J. Anim. Sci. 2004. 82:32-40. C. D. Gladney, G. R. Bertani, R. K. Johnson and D. Pomp. 2004. Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR and human microarrays: I. Ovarian follicles. J. Anim. Sci. 2004. 82:17-31. Petry, D. B., J. Holl, J. Weber, A. Doster, F. Osorio, and R. Johnson. 2004. Different biological responses of pigs of two genetic populations to PRRSV challenge suggests underlying genetic variation in susceptibility/resistance to PRRSV. NE Swine Report, EC 04-219 A: 24-28. Holl, J. and R. Johnson. 2004. Major genes affect reproduction and early growth. 2004. NE Swine Report, EC 04-219 A: 28-31. *J. Holl, J. P. Cassady, D. Pomp , and R. K. Johnson. 2004. Identification of quantitative trait loci affecting reproduction and early growth in pigs. J Anim. Sci. Vol. 82, Suppl. 1: 452. D. B. Petry, J. W. Holl, F. A. Osario, A. R. Doster, J. S. Weber, and R. K. Johnson. 2004. Biological responses of pigs from two populations to PRRSV challenge. Journal of Animal Science Vol. 82, Suppl. 2: 39. *J. W. Holl, J. P., Cassady, and R. K. Johnson. 2004. Identification of quantitative trait loci affecting birth and weaning weights in pigs. J. Anim. Sci. Vol. 81, Suppl. 1: 195. NORTH CAROLINA *Cassady, J. P., O. W. Robison, R. K. Johnson, J. W. Mabry, L. L. Christian, M. D. Tokach, R. K. Miller, R. N. Goodwin. National Pork Producers Council Maternal Line Genetic Evaluation: A comparison of growth and carcass traits in terminal progeny. Journal of Animal Science. 82(12): 3482-5, 01 Dec 2004. *Holl, J. W., J. P. Cassady, D. Pomp, R. K. Johnson. A genome scan for quantitative trait loci and imprinted regions affecting reproduction in pigs. Journal of Animal Science. 82(12): 3421-9, 01 Dec 2004. House, R.L., J.P. Cassady, E.J. Eisen, T.E. Eling, and J. Odle. Functional genomic characterization of polygenic obese mice fed t10, c12 conjugated linoleic acid (CLA). FASEB J. 18(4&5), 2004. *Van Vleck, L. D., J. P. Cassady. Unexpected estimates of variance components with a true model containing genetic competition effects. Journal of Animal Science. 2005 Accepted. Walker, S. E., O.W. Robison, C.S. Whisnant, and J.P. Cassady. Effect of divergent selection for testosterone production on testicular morphology and daily sperm production in boars. Journal of Animal Science. 82(8): 2259-63, 01 Aug 2004. Bender, J.M., J.P. Cassady. 2004. Growth and carcass composition in lines divergently selected for testosterone production and their crossbred progeny. Journal of Animal Science Supplement 1 *Van Vleck, L.D., J.P. Cassady. 2004. Random models with direct and competition genetic effects. Proceedings, 2004 Conference on Statistics in Agriculture, Manhattan, KS *Van Vleck, L.D., J.P. Cassady. 2004. Unexpected estimates of variance components with a true model containing genetic competition effects. Journal of Animal Science Supplement 1 *Cassady, J.P., L.D. Van Vleck. 2004. Estimation of (co) variance components due to genetic competition effects in pigs. Journal of Animal Science Supplement 1 *Holl, J.W., J.P. Cassady, R.K. Johnson. 2004. Identification of quantitative trait loci affecting birth and weaning weights in pigs. Journal of Animal Science Supplement 1 *Holl, J.W., J.P. Cassady, D. Pomp, R.K. Johnson. 2004. Identification of quantitative trait loci affecting reproduction and early growth in pigs. Journal of Animal Science Supplement 1 Blowe, C.D., E.J. Eisen, O.W. Robison, and J.P. Cassady. 2004. Characterization of a line of pigs selected for increased litter size for two RFLPs identified in follistatin C. D. Blowe, E. J. Eisen, O. W. Robison, and J. P. Cassady. Journal of Animal Science Supplement 1 OHIO *Moeller, S. J., R. N. Goodwin, R. K. Johnson, J. W. Mabry, T. J. Baas, O. W. Robison. 2004. The National Pork Producers Council Maternal Line National Genetic Evaluation Program: A comparison of six maternal genetic lines for female productivity measures over four parities. J. Anim. Sci. 82: 41-53 OKLAHOMA Vonnahme,K.A., Fernando,S.C., Ross,J.W., Ashworth,M.D., DeSilva,U., Malayer,J.R., and Geisert,R.D. (2004). Porcine endometrial expression of kininogen, factor XII, and plasma kallikrein in cyclic and pregnant gilts. Biology of Reproduction 70, 132-138 USDA-BARC Martens GW, Lunney JK, Baker JE, Smith DM. 2003. Rapid Assignment of Swine Leukocyte Antigen (SLA) Haplotypes in Pedigreed Herds using a Polymerase Chain Reaction Based Assay. Immunogenetics. 55: 395-401. Dawson HD, Beshah E, Nishi S, Solano-Aguilar G, Morimoto M, Zhao A, Madden KB, Ledbetter TK, Dubey JP, Shea-Donohue T, Lunney JK, Urban, JF Jr. Localized multi-gene expression patterns support an evolving Th1/Th2-like paradigm in response to infections with Toxoplasma gondii and Ascaris suum in pigs. Infection and Immunity. In Press. *Smith DM, Martens GW, Lunney JK, Ando A, Lee J-H, Ho C-S, Schook L, Renard C, Chardon P. 2004. Nomenclature for Factors of the SLA Class I System, 2004. Tissue Antigens. In Press. Dawson HD, Royaee AR, Nishi S, Kuhar D, Schnitzlein WM, Zuckermann F, Urban JF, Lunney JK. 2004. Identification of Key Immune Mediators Regulating T helper 1 Responses in Swine. Vet. Immunol. Immunopathol. 100: 105-111. Royaee AR, Husmann R, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann F, Lunney JK. 2004. Deciphering the involvement of innate immune factors in the development of the host responses to PRRSV vaccination. Vet. Immunol. Immunopathol. 102: 199-216. Meier WA, Husmann RJ, Calzada-Nova G, Schnitzlein WM, Lunney JK, Zuckermann FA. 2004. In vivo modulation of the PRRS virus vaccine-induced immune responses of swine toward a Th1 phenotype. Vet. Immunol. Immunopathol. 102: 299-314. Galina-Pantoja L, Solano-Aguilar GI, Mellencamp MA, Bastiaansen J, Lunney JK. 2004. Relationship between immune cells and pig growth on a commercial farm. Proc. 18th IPVS Congress, Hamburg, Germany, 2004, Vol. I. p.381. Bannerman D, Paape MJ, Lunney JK. 2004. Immunity: Innate. In Encyclopedia of Animal Science; Pond, W.G., Bell, A.W. Eds.; Marcel Dekker, Inc., New York. p.552-554. Lunney JK, Paape MJ, Bannerman D. 2004. Immunity: Acquired. In Encyclopedia of Animal Science; Pond, W.G., Bell, A.W. Eds.; Marcel Dekker, Inc., New York. p.548-551.