SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

Dennis Calvin, PSU, dcalvin@psu.edu; Tom Hunt, University of Nebraska, Haskell Ag Lab, thunt2@unl.edu; Pat Porter, Texas A & M, p-porter@tamu.edu; Dave Andow, University of Minnesota, dandow@umn.edu; Chuck Mason, University of Delaware, mason@udel.edu; Larry Buschman, Kansas State Univ., lbuschma@ksu.edu; Jocelyn Smith, University of Guelph, jsmith@ridgetownc.uoguelph.ca; David Buntin, University of Georgia, gbuntin@uga.edu; Tom Sappington, USDA-ARS, CICGRU, Ames IA, Tom.Sappington@ars.usda.gov; Rick Hellmich, USDA-ARS, CICGRU, Ames IA, Richard.Hellmich@ars.usda.gov; Erica Lindroth (student), Univ. of NE, erica.lindroth@huskers.unl.edu; Tracy Baute, OMAFRA Canada, tracey.baute@ontario.ca; Andy Michel, OARDC-Ohio State University, michel.70@osu.edu; Bill Hutchinson, University of Minnesota, hutch002@umn.edu; Chris DiFonzo, MSU, difonzo@msu.edu; John Glaser, EPA, glaser.john@epa.gov; Eileen Cullen, University of Wisconsin, cullen@entomology.wisc.edu; Blair Siegfried, University of Nebraska-Lincoln, bsiegfried1@unl.edu; Silvana Paula-Moraes (student), silvana.moraes@huskers.unl.edu; Brian McCornack, Kansas State University, mccornac@ksu.edu; Janine Razze (student), University of Delaware, jrazze@udel.edu;

NIMSS Minutes Summary, Jan 09 NC-205 Meeting Annual Meeting of the Multi-State Regional Research Committee NC-205 Ecology and Management of European Corn Borer and Other Lepidopteran Pests of Corn As recorded by Tom Hunt, secretary. Minneapolis, MN, January 29- 30, 2009 Radisson University Hotel 615 Washington Ave. SE Minneapolis, MN 55414 (800) 822-6757 Thursday, January 29, 2009 Chair of the NC-205 committee, Dennis Calvin, opened the meeting at 8:30 a.m. Committee secretary, Tom Hunt, took minutes. Local Arrangement Announcements Dave Andow indicated registration fee would be $100 for the NC205 meeting, $150 if you also attended the NCCC46/NC205 joint meeting, payable to the University of Minnesota. Time and Place and Nominating Committees established Dave Andow and Eileen Cullen volunteered to be the T and P Committee. Eileen conveyed that NCCC46 suggested we meet in Madison, Wisconsin in 2010. In 2010, NC205 would meet January 25-26, a joint NC205/NCCC46 meeting would be held Janaury 27, and NCCC46 would meet January 28-29. Siegfried suggested we meet in conjunction with the IWGO (ECB and Diabrotica in Europe) in 2011 in Europe, as there are natural collaborations and shared interests between the groups. Various logistical issues were discussed. The IWGO would organize most details of the meeting. Costs could be comparable to some U.S. meeting locations. Andow noted the Administrative Advisor would have to agree. Siegfried and Sappington will do the initial groundwork in April at the Munich meeting of the IWGO. An informal vote indicated that the majority (10) of attending committee members were interested. It was suggested we may pursue a USDA Conference Grant to defray the costs for some members. Larry Buschman motioned we accept Madison, Wisconsin, January 25-26, 2010 as the next annual meeting of NC205. Rick Hellmich seconded and the motion carried. Rick Hellmich and Charles Mason volunteered to be the Nominating Committee. Administrative Advisor Report Steve Pueppke was called away, so Calvin initiated discussion of AFRI, Smith-Lever IPM, and the erosion of formula funds. Porter noted that with the change in Smith-Lever IPM funding, there will be a significant decline in capacity and impact for both field personnel and faculty. Mason suggested recruiting Calvin as next administrative advisor to new NC205 project, as he has been involved with ECB and NC205 for so long. Hunt will talk to Steve Pueppke about the possibility. NCR 327 Progress Mason indicated we need to push on to the finish. Publication date is in 2010. Sections were shifted. Final sections to Mason by Feb 28. Final draft will go to working committee by March 1 to be edited by April 30. In June the final draft will be sent to full committee. Remember to include major citations. Recap of Activities and Influence Andow is involved with developing biological risk assessment capacity in East Africa, Asia, and South America. A book, Environmental Risk Assessment of Genetically Modified Organisms. V4. Challenges and Opportunities with Bt Cotton in Vietnam, was published. Buntin noted that the southern corn entomologists have formed a working group because corn acreage is increasing. Siegfried noted that a U.S. Aid project developing management information for Asian corn borer is incorporating our IRM work into their material. Sappington spoke at the China National Academy of Sciences migrating insect group on ECB and explored possible collaborations. Baute noted their influence on the regulatory process in Canada and their Respect the Refuge campaign. Porter was interviewed for the Seed Trade Industry News on stacks. DiFonzo is collaborating with the Philippines in training extension personnel in corn (ACB). Hellmich published a book chapter the role of transgenics past resent and future in IPM and is involved with other International scientists on developing a common-sense approach to examining non-target effects. Mason noted we all do a lot of work with Industry and often invite them to our meetings. NC205 Project Rewrite It was noted our current title suits the new project. Floor was opened for ideas/directions for new project. Various broad areas should possibly be considered, including environmental issues, green technology, the evolution of IRM, information delivery, the growing importance of other leps. (ear pests), resistance issues, the impact of transgenics on other target pests (other than ECB), and refuge evaluation. Members noted their specific research areas. Calvin summarized the applied and basic research topics as ear feeding pests and their increase (biology, genetics, ecology, and management), resistance monitoring, ECB and other lep. genomics, insect movement, mating biology (specific and models), natural enemies and biological control, extension (better delivery), pheromone and voltine types, area-wide suppression, organic management, emerging transgenics (other proteins, etc.), and the inclusion of other tolerance genes (cold, drought). 12:00  1:30 (approx.) LUNCH Rewrite continued Objectives were developed, writing teams were established, and deadlines set. State Reports & Discussion, by Objective. Research, extension, and outreach reports were presented by state representatives to NC-205. Discussion ranged over a variety of NC-205 objective-related topics. 5:00 ADJOURN for the day. Friday, January 30, 2009 Continuation of State Reports & Discussion Return to Committee Business Mason motioned to recommend Calvin as Administrative Advisor for new project. Hellmich seconded. Committee voted yes. John Glasers request for a letter from NC205 supporting his research was discussed. Committee voted yes, and suggested Calvin and Hellmich draft a letter and send it to committee for consideration. The next joint meeting of NC205/NCC46 (2010) should be closed, at least in part so the committees can speak freely. Minutes (2008 meeting) were approved (Andow motion, Mason second). Nominating Committee Hellmich motioned Calvin and Hunt be elected Chair and Secretary 2010, respectively. Mason seconded, yes vote carried. Mason motioned Hunt and Cullen be elected Chair and Secretary for first year of next project, respectively. Hellmich seconded, yes vote carried. Continuation of State Reports & Discussion Continuation of General Business Send outcomes and impacts to Calvin. There is much confusion with respect to IRM, and it will only grow. We have an opportunity to make an impact in this area. An invitation to start an IRM subcommittee was extended. Those interested are: Andow, Cullen, Porter, Buschman, Sappington, Hellmich, Michel, and Hunt. 12:00 ADJOURN 2009 NC-205 Annual Meeting

Accomplishments

Andow (Minn) is involved with developing biological risk assessment capacity in East Africa, Asia, and South America. A book, Environmental Risk Assessment of Genetically Modified Organisms. V4. Challenges and Opportunities with Bt Cotton in Vietnam, was published. Buntin (GA) noted that the southern corn entomologists have formed a working group because corn acreage is increasing. Siegfried (NE) noted that a U.S. Aid project developing management information for Asian corn borer is incorporating our IRM work into their material. Sappington (IA) spoke at the China National Academy of Sciences migrating insect group on ECB and explored possible collaborations. Baute (Ontario) noted their influence on the regulatory process in Canada and their Respect the Refuge campaign. Porter (TX) was interviewed for the Seed Trade Industry News on stacks. DiFonzo (Mich) is collaborating with the Philippines in training extension personnel in corn (ACB). Hellmich (IA) published a book chapter the role of transgenics past resent and future in IPM and is involved with other International scientists on developing a common-sense approach to examining non-target effects. Andow (MINN) reported that F2 screens for Heliothus armigera are being used. The percent of resistance in cotton in China is increasing in frequency. Heliothus virescens F2 screens are being developed. F2 screen theory development is leading to pedigree screens. A publication is in press on male ECB reproductive system. An EST library for western bean cutworm is being developed (IA), and SNPs are being developed as markers. Several manuscripts were published on edge effects (MINN), Macrocentrus grandii, and mating frequency in Coleomegilla maculate. Corn earworm website is expanding (MINN), as is the trapping network. Cooperative studies on the Bt resistance mechanisms in sugarcane borer have been established among several institutions in the United State, Spain, and Germany. A resistance allele to Cry1Ab corn has been detected in sugarcane borer, a significant corn pest. To address this issue, a regional research project was initiated in 2008 by a research group from LSU AgCenter, Texas A&M, and Mississippi. Genetic studies were conducted on lab-selected ECB that are resistant to Cry1Ab, an insecticidal protein produced by most Bt corn plants. An AFLP linkage map was developed including 253 markers on 31 autosomal chromosomes. These efforts may substantially accelerate efforts to prevent the development of ECB resistance to Bt corn plants by mapping chromosomal segments related to resistance. Nosema pyrausta a natural enemy of ECB, was found to significantly impact Cry1Ab-resistant ECB populations. Significant genetic variation was quantified in the laboratory for tolerance to infection by this microsporidium. Selection for increased tolerance by populations of the ECB will compromise Nosema's ability to control ECB. The Corn Insects and Crop Genetics Research Unit has impacted technical and regulatory programs and has helped establish an international reputation in insect resistance management, evaluation of possible non-target effects of genetically modified crops, and insect genetics and ecology. Carbon isotope analysis revealed that alternative host plant use by E strain European corn borer, Ostrinia nubilalis, (ECB) is relatively high compared to Z strain, a study with important and far-reaching implications for insect resistance management for this insect in Bt corn. The demonstration that the Z strain ECB overlaps everywhere the E strain occurs, and the Z strain's low use of alternative hosts by the latter indicate that relaxing of structured refuge requirements on the basis of natural refuge is not justified and has helped guide EPA policy decisions on petitions to modify refuge constraints. Demonstration that ECB bivoltine Z strain moths commonly disperse >100 km exposed the potential importance of E strain moths in the eastern U.S. in development of resistance to Bt corn.

Impacts

  1. Resistance to pesticides continues to be a concern, and will become an even greater concern to agriculture in the future. By educating farmers to the importance of this issue, IRM compliance has been increased and our current and future management tools will be preserved well into the future, protecting the environment and contributing to farmer profitability.
  2. Planting of Bt corn has increased dramatically since its introduction in 1996. Widespread adoption of the technology will likely increase selection pressures and place increased priority on development of sound resistance management practices. Bt resistance monitoring information provided by NC-205 participants is currently utilized by most of the major seed and biotechnology companies to support registrations of transgenic corn for both European corn borer and more recently, the western corn rootworm. These data are necessary to the long term sustainability of this technology.
  3. Surveys of participants in the UNL Extension Crop Protection Clinics, where corn IPM and IRM presentations were made, indicated that 1,500 agribusiness representatives and producers were trained on profitable pest management strategies. Over 50% of the attendees responded that the program helped them learn new material that will improve their operations. Participants demonstrated an average improvement of 86% in understanding selected content as measured by pre- and post-quiz questions. Potential value of the clinics is $28 million. Likewise, surveys of the UNL Extensions 2008 Crop Management Diagnostic Clinics indicated that the participants valued the material at more than $30 million dollars, and the 527 Ag producers and agribusiness representatives trained had a 100% satisfaction level.
  4. The F2 screen is now being used to screen for Bt resistant alleles for Heliothis virescens in SE US and for Helicoverpa armigera in China and continues to be used for Ostrinia nubilalis in France and for H. armigera in Australia (for both Cry1Ac and Cry2Ab).
  5. Ecological risk assessment for GM crops is being conducted by about 50 trained Vietnamese scientists.
  6. Education of Michigan growers on Bt technology, particularly on stacked traits, will reduce loss from poor or incorrect seed choice, and reinforce the importance of managing resistance. Pheromone trapping documented the first successful overwintering survival of western bean cutworm (WBC) and statewide infestation. WBC damage to corn and dry beans was economically significant through yield loss and insecticide cost. This new pest will necessitate new recommendations and research in 2009.
  7. The link between the Philippines and MSU assisted that country in developing resistance management policies for Asian corn borer, and in building the capacity of government employees tasked with regulating Bt corn. This will help the Philippines use biotechnology more wisely.
  8. Corn growers in North Dakota have increased their knowledge on and utilization of Integrated Pest Management strategies for control of European corn borer as evidenced by improved timing of field scouting activities and treatment decisions and increased understanding of use of Bt transgenic corn and its implications for resistance management of European corn borer.

Publications

Alves, A.P., W.J. Allgeier, and B.D. Siegfried. 2008. Effects of the synergist S,S,S tributyl phosphorotrithioate on indoxacarb toxicity and metabolism in the European corn borer, Ostrinia nubilalis (Hubner). Pestic. Biochem. Physiol 90: 26-30. Andow, D. A., G. P. Fitt, E. E. Grafius, R. E. Jackson, E. B. Radcliffe, D. W. Ragsdale and L. Rossiter. 2008. Pesticide and transgenic plant resistance management in the field, pp. 118-145. In M. E. Whalon, D. Mota-Sanchez and R. M. Hollingsworth (eds.), Global Pesticide Resistance in Arthropods. CAB International, Wallingford, UK. Andow, D. A. 2008. The risk of resistance evolution in insects to transgenic insecticidal crops. Collection of Biosafety Reviews 4: 142-199. http://www.icgeb.org/biosafety/publications/collections.html Blanco, C. A., O. P. Perera, F. Gould, D. V. Sumerford, G. Hernandez, C. A. Abel and D. A. Andow. 2008. An empirical test of the F2 screen for detection of Bacillus thuringiensis resistance alleles in tobacco budworm (Lepidoptera: Noctuidae). Journal of Economic Entomology 101: 1406 1414. Blanco, C.A., N.P. Storer, C.A. Abel, R. Jackson, R. Leonard, J.D. Lopez, G. Payne, B.D. Siegfried, T. Spencer, and A.P. Teran Vargas. 2008. Baseline susceptibility of tobacco budworm (Lepidoptera: Noctuidae) to Cry1F toxin from Bacillus thuringiensis. J. Econ. Entomol. 101: 168-173. Buschman, L.L., H. Davis and P. Sloderbeck. 2008. Efficacy of Monsanto stacked event corn hybrids for control of southwestern corn borer and corn earworm, 2007. KSU SW Res-Ext. Center 2008 Field Day Report of Prob. 997: 64-68. Carroll, M. W., J. A. Glaser, R. L. Hellmich, T. E. Hunt, T. W. Sappington, D. Calvin, K. Copenhaver and J. Fridgen. 2008. Use of Vegetative Indices Derived from Airbourne Hyperspectral Imagery for Detection of European Corn Borer Infestation in Iowa Corn Plots. J. Econ. Entomol. 101: 1614-1623. Coates B.S., Sumerford D.V., Lewis L.C. 2008. Segregation of Ostrinia nubilalis aminopeptidase 1 (APN1), cadherin, and bre5-like alleles from a Cry1Ab resistant colony are not associated with F2 larval weights when fed on toxin-containing diet. J. Insect Sci. 8:21. Coates B.S., Sumerford D.V., Hellmich R.L., Lewis L.C. 2008. Mining an Ostrinia nubilalis midgut expressed sequence tag (EST) library for candidate genes and single nucleotide polymorphisms (SNPs). Insect Mol Biol 17(6):607-621. Crespo, A.L.B., T.A. Spencer, E. Nekl, and B.D. Siegfried. 2008. Comparison and validation of methods to quantify Cry1Ab from Bacillus thuringiensis for standardization of insect bioassays. Appl. Environ. Microbiol. 74: 130-135. DiFonzo, C.D., M. Jewett, F. Warner, D. Brown-Rytlewski, and W. Kirk. 2008. Insect, nematode, and disease control in field and forage crops. MSU Bulletin E-1582. DiFonzo, C.D. and R. Hammond. 2008. Range expansion of western bean cutworm, Striacosta albicosta (Noctuidae), into Michigan and Ohio. Crop Management. doi:10.1094/CM-2008-0519-01-BR. Dorhout, D. L., T. W. Sappington, and M. E. Rice. 2008. Evidence for obligate migratory flight behavior in young European corn borer (Lepidoptera: Crambidae) females. Environ. Entomol. 37: 1280-1290. Harmon, J. P., A. Hyden and D. A. Andow. 2008. Absence makes the heart grow fonder: Isolation enhances the frequency of mating in Coleomegilla maculata (Coleoptera: Coccinellidae). Journal of Insect Behavior 21: 495 504. Hilbeck, A., S. Arpaia, A.N.E. Birch, Y. Chen, E.M.G. Fontes, A. Lang, Le Thi Thu Hong, G.L. Lovei, B. Manachini, Nguyen Thi Thu Cuc, Nguyen Van Huyhn, Nguyen Van Tuat, Pham Van Lam, Pham Van Toan, C.S.S. Pires, E.R. Sujii, Trac Khuong Lai, E. Underwood, R.E. Wheatley, L.J. Wilson, C. Zwahlen, and D.A. Andow. 2008. Non-target and biological diversity risk assessment, pp. 115-137. In D.A. Andow, A. Hilbeck and Nguyen Van Tuat (eds), Environmental Risk Assessment of Genetically Modified Organisms: Challenges and Opportunities with Bt Cotton in Viet Nam. CABI Publishing, Wallingford, UK. Huang, F., R. Parker, B.R. Leonard, Y. Yong, and Jin Liu. 2009. Frequency of resistance alleles to Bacillus thuringiensis-corn in Texas populations of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae). Crop Protection. doi:10.1016/j.cropro.2008.10.002. Huang, F., B.R. Leonard, S.H. Moore, B. Yue, R. Parker, T. Reagan, M. Stout, D.R. Cook, W. Akbar, C. Chilcutt, W. White, D. Lee, and S. Biles. 2008. Geographical susceptibility of Louisiana and Texas populations of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae) to Bacillus thuringiensis Cry1Ab protein. Crop Protect. 27: 799-806. Hunt, T., K. Jarvi, R. Wright and G. Hein. 2008. Integrated Pest Management: As important now as ever. pp. 47-50, Proceedings of Crop Protection Clinics. University of Nebraska-Lincoln Extension. Hurley, T. M., and P. D. Mitchell. 2008. Insect Resistance Management: Adoption and Compliance. D. W. Onstad, ed. Insect Resistance Management: Biology, Economics, and Prediction. San Diego, CA: Academic Press, pp. 227-253. Icoz, I., Saxena, D., Andow, D. A., Zwahlen, C. and Stotzky, G. 2008. Microbial populations and enzyme activities in soil in situ under transgenic corn expressing Cry proteins from Bacillus thuringiensis. Journal of Environmental Quality 37: 647 662. Knodel, J.J., P. Beauzay, M. Boetel, and D. Markle. 2008. 2008 North Dakota Field Crop Insect Management Guide. NDSU Ext. Serv., E-1143. Krumm, J. T., T. E. Hunt, S. R. Skoda, G. L. Hein, D. L. Lee, P. L. Clark, and J. E. Foster. 2008. Genetic variability of the European corn borer, Ostinia nubilalis, suggests gene flow between populations in the Midwestern United States. J. Insect Science 8:69, available online: insectscience.org/8.69. Mitchell, P. D., and W. D. Hutchison. 2008. Assessing and Communicating the Value and Risk of IPM. E. B. Radcliffe and W. D. Hutchison, eds. Integrated Pest Management. Cambridge, UK: Cambridge University Press. Forthcoming. Mitchell, P. D., and D. W. Onstad. 2008. Valuing Insect Resistance in an Uncertain Future. D. W. Onstad, ed. Insect Resistance Management: Biology, Economics, and Prediction. San Diego, CA: Academic Press, pp. 17-38. Olson, D.M. and D.A. Andow. 2008. Patch size and edges and insect populations in landscapes. Oecologia 155: 549 558. Pereira, E.J.G., B.A. Lang, N.P. Storer, and B.D. Siegfried. 2008. Selection for Cry1F resistance in the European corn borer and cross resistance to other Cry toxins. Entomol. Exper. Appl. 126: 115-121. Pereira, E.J.G., N.P. Storer, and B.D. Siegfried. 2008. Inheritance of Cry1F resistance in laboratory-selected European corn borer and its survival on transgenic corn expressing the Cry1F toxin. Bull. Entomol. Research (doi:10.1017/S0007485308005920). Palizada, S. A. 2008. Effects of different transgenic and non-transgenic maize on minute pirate bug (Orius insidiosus (Say)) populations and other key arthropods. M.S. Thesis., University of Nebraska-Lincon, Lincoln, Nebraska. Prasifka, J.R., Lopez, M.D., Hellmich, R.L. 2008. Effects of insecticide exposure on movement and population size estimates of predatory ground beetles (Coleoptera: Carabidae). Pest Management Science. 64:30-36. Prasifka, J.R., R.L. Hellmich, G.P. Dively, L.S. Higgins, P.M. Dixon and J.J. Duan. 2008. Selection of nontarget arthropod taxa for field research on transgenic insecticidal crops: Using empirical data and statistical power. Environ. Entomol. 37:1-10. Romeis, J., D. Bartsch, F. Bigler, M.P. Candolfi, M.M.C. Gielkens, S.E. Hartley, R.L. Hellmich, J.E. Huesing, P.C. Jepson, R. Layton, H. Quemada, A. Raybould, R.I. Rose, J. Schiemann, M.K. Sears, A.M. Shelton, J. Sweet, Z. Vaituzis and J.D. Wolt. 2008. Assessment of risk of insect-resistant transgenic crops to nontarget arthropods. Nature Biotech. 26:203-208. White, J.A. and D.A. Andow. 2008. Benefits of self-super-parasitism in a polyembryonic parasitoid. Biological Control 46:133 139. Wu, X., B.R. Leonard, Y-C Zhu, C.A. Abel, G.P. Head, and F. Huang. 2009. Susceptibility of Cry1Ab-resistant and -susceptible sugarcane borer (Lepidoptera: Crambidae) to four Bacillus thuringiensis toxins. J. Invert. Pathol. 100:29-34. Yue, B., F. Huang, B. R. Leonard, S. Moore, D. A. Andow, D. Cook, K. Emfinger and D. R. Lee. 2009. Verifying an F1 screen for identification and quantification of rare Bacillus thuringiensis resistance alleles in field populations of sugarcane borer, Diatraea saccharalis. Entomologia Experimentalis et Applicata 129: 172 180. Ziems, J. R., W. W. Hoback, L. G. Higley, T. E. Hunt, O. A. Fernandes, C. Bastos, and A. Bueno. 2008. Second generation European corn borer (Lepidoptera: Crambidae) injury and Irish potato physiology, yield, and quality. Agron. J. 100:720-725.
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