SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

John Parrish [parrish@ansci.wisc.edu] Wisconsin; Dave Guthrie [dave.guthrie@ars.usda.gov] USDA-BARC; Matt Wilson [Wilson@mail.wvu.edu] West Virginia; Lloyd L Anderson [llanders@iastate.edu] Iowa ; Timothy J. Safranski [SafranskiT@missouri.edu] Missouri; Joe Ford [ford@email.marc.usda.gov] USDA-MARC ; Rebecca Krisher [rkrisher@illinois.edu] Illinois; Duane Davis [ddavis@oznet.ksu.edu] Kansas ; Mark Diekman [mdiekman@purdue.edu] Indiana ; Brett White [bwhit1@unlnotes.unl.edu] Nebraska ; John Baker [baker@anr.msu.edu] Michigan;

The annual meeting of the NCERA-57 was called to order by Mark Diekman, local host, at 8:15 AM on Wednesday June 25th. Mark introduced Chuck Hibberd, Associate Dean & Director of Cooperative Extension Service. Chuck welcomed us to campus and gave us a brief update on the Purdue University Extension Service and how he was working towards a focus on Transformation Extension Programs. The goal is to raise the bar from level 1 one-on-one contacts to level 4 transformational extension programs.

Mark then introduced Alan Grant, Department Head of Animal Science. Alan spoke about recent trends in animal agriculture in Indiana and the Governors goals for increasing animal agriculture in Indiana. He indicated that in the face of a 30% decrease in pig numbers in the last 17 years, the number of feeder pigs had increased 1000% indicating a shift in the nature of the swine industry in the state. He also indicated that over the same time period the undergraduate enrollment in Animal Science had increased from ~200 in 1990 to ~600 undergraduate students in 2008. Discussion ensued about the demographics and interests of those students and that similar trends have been observed at other stations.

John Baker, Associate Director of the Michigan Agricultural Experiment Station and Administrative Advisor to NCERA-57, addressed the need to begin the process of rewriting the NCERA-57 project. He indicated that he could give editorial rights to three individuals. We codified the previously discussed concept that John Parrish would chair the rewrite committee and that Matt Wilson and Dave Guthrie would fill out the committee. John distributed a handout containing pertinent information for the rewrite effort and the deadlines for the various components of the renewal. He emphasized the need for impact statements and commended our project for their success, including the submission and planned resubmission of an integrated grant proposal to USDA- NRICGP and the biennial symposium.

Station reports then began. Duane Davis spoke about the online lab class that he has been developing at Kansas State to replace their wet lab for online students. He indicated that aspects of this would fit into the teaching component of the integrated project resubmission. One of his significant questions was how to best use modules for on and off campus. Station reports continued until about 11 at which time we travelled to JBS United in Sheridan, IN for the symposium organized by the regional project on Sow Longevity.

The business meeting was called to order on June 26th. David Miller was unanimously elected the next Secretary for NCERA-57. West Virginia University was selected as the host institution for the next annual meeting and dates were discussed. If acceptable, the group settled on the 24th and 25th of June, 2009 with June 23rd serving as a travel day.

Plans for the next symposium were initiated and a tentative topic of gilt development from prenatal to mating was arrived at. John Parrish asked that all members send him brief impact statements that he could use to initiate the renewal process. Finally, the regional committee thanked Mark Diekman and Purdue University for graciously hosting the annual meeting and letters of thanks will be sent to Mark and to JBS United for hosting and supporting the biennial symposium.

Dave Guthrie spoke briefly about the grant resubmission. He indicated that the two main issues were the proposed dose of the synchronization compound and the need for teaching and economic analyses. He also indicated that he would again be soliciting letters of support.

Station reports then resumed with reports from Bret White, Lloyd Anderson, Tim Safranski, John Parrish, Joe Ford and Rebecca Krisher.

The meeting was adjourned at 11:00 and the gavel was virtually passed from John Parrish to Dave Guthrie.

Respectfully submitted Matthew Wilson, Secretary, NCERA-57

Accomplishments

1. The NCERA-57 committe, with leadership from Dave Guthrie and Duane Davis, revised the integrated proposal entitled Seasonal Infertility in Swine: Research and Educational Goals to Enhance the Profitability and Sustainability of the Swine Industry that was not funded in 2007, unfortunately it was not submitted in time for the 2007 grant cycle. Work continues to proceed relative to additional refinement of the proposal and it should be resubmitted in 2008. 2. The committee organized and hosted its biennial symposium entitled Sow Longevity. The focus of the symposium was to extend the efforts of the committee and others to extension faculty, producers and individuals affiliated with the swine industry. Greater that 60 attendees pack the conference room at JBS United for what was an excellent series of presentations and discussion. The slides presented will be put together on to CDs that will be distributed to the committee, extension agents and interested individuals. The presentations should also be available via the website that John Parrish continues to develop to serve as a repository of information and tools for and from the committee. 3. A topic was identified for the next symposium that will focus on animal management from the prenatal period through breeding.

Impacts

  1. Modules have been developed and will be distributed that help institutions transition from wet laboratories to online laboratories for courses in animal reproduction, including swine reproduction.
  2. Artificial insemination in swine with frozen semen has been problematic. The committe has better defined the window of opportunity for utilizing frozen semen for fixed-time AI. Efforts continue to develop better cryopreservation methodologies for AI.
  3. Members of the committee have describe the genetic variation that exists in the GnRH promoter amongst lines of pigs with differing ovulation rates. Potential exists then to exploit this new understanding to improve reproductive efficiency.
  4. The secretion of proteins is a basic function required of almost every cell in the body and contributes to the regulation of all of endocrinology. Details of the basic mechanisms of cellular secretion of proteins have been extended by members of the committee and will help elucidate a vast array of endocrine mechanisms.
  5. Fourier harmonic analysis has been refined to better describe fertility of a semen sample. Specifically, two components of the fourier harmonics allow a very high level of predictability as to whether a particular boar will have acceptable or unacceptable fertility.

Publications

Sagirkaya H, Misirlioglu M, Kaya A, First NL, Parrish JJ, Memili E. Developmental potential of bovine oocytes cultured in different maturation and culture conditions. Anim Reprod Sci. 2007 Oct;101(3-4):225-40. Jerde TJ, Mellon WS, Bjorling DE, Checura CM, Owusu-Ofori K, Parrish JJ, Nakada SY. Stretch induction of cyclooxygenase-2 expression in human urothelial cells is calcium- and protein kinase C zeta-dependent. Mol Pharmacol. 2008 Jan;73(1):18-26. Siddiqui MA, Bhattacharjee J, Das ZC, Islam MM, Islam MA, Haque MA, Parrish JJ, Shamsuddin M. Crossbred bull selection for bigger scrotum and shorter age at puberty with potentials for better quality semen. Reprod Domest Anim. 2008 Feb;43(1):74-9. K.L. Willenburg, K.J. Rozenboom and J.J. Parrish. The relationship between sperm nuclear shape and boar fertility using fourier harmonics. Journal of Animal Science, Volume 86, E-Supplement 2, 2008. p 525. J. A. Sterle and J. J. Parrish. Changes that have occurred in animal science teaching. Journal of Animal Science, 2008. Volume 86, E-Supplement 2, p 345. J. J. Parrish. The use of podcasts in the classroom. Journal of Animal Science, Volume 86, E-Supplement 2, 2008. p 346. J.J. Parrish, C. Mueller, E. Ludwig and J. L. Susko-Parrish. Day length does not affect live sperm nuclear shape in the stallion. Reproduction, Fertility and Development. 2008 20:192. Lefric Enwall and John Parrish. Effect of scrotal insulation on bull sperm in vitro fertility, nuclear shape and apoptotic markers. Journal of Andrology Supplement, Proceedings of the American Society of Andrology 33rd Annual meeting. 2008. p 47. Antelman, J., G. Manandhar, Y.-J. Yi, R. Li, K. Whitworth, J.A. Green, R.S. Prather, P. Sutovsky. 2008. Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development. J. Cellular Physiology. (accepted 6-3-08). Bonk, A.J., H.-T. Cheong, R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason, K.M. Whitworth, C.N. Murphy, E. Antoniou, R.S. Prather. 2007. Correlation of developmental differences to the methylation profiles of nuclear transfer donor cells. Epigenetics 2:3, 179-186. Bonk, A.J., R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason, K.M. Whitworth, C.N. Murphy, E. Antoniou, R.S. Prather. 2008. Aberrant DNA methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transfer-produced embryos. Mol. Reprod. Dev. 75:250-264. Caamaño, J.N., A. Rodriguez, A. Salas, M. Muñoz, C. Diez, R.S. Prather, E. Goméz. 2008. Flow cytometric cell cycle analysis of cultured brown bear fibroblasts. Cell Biology International (accepted). Hao, Y., C. Murphy, L. Spate, D. Wax, Z. Zhong, M. Samuel, N. Mathialagan, H. Schatten, R.S. Prather. 2008. Osteopontin improves in vitro development of porcine embryos and decreases apoptosis. Mol. Reprod. Dev. 75:291-298. Im, G.-S., M. Samuel, L. Lai, Y. Hao, R.S. Prather. 2007. Development and calcium level changes in pre-implantation porcine nuclear transfer embryos activated with 6-DMAP after fusion. Mol. Reprod. Dev. 74:1158-1164. Isom, S.C., R.S. Prather, E.B. Rucker III. 2007. Heat stress-induced apoptosis in porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos. Mol. Reprod. Dev. 74:574-581. Jiang, L., P. Jobst, L. Lai, M. Samuel, D. Ayers, R.S. Prather, X.C. Tian. 2007. Expression of growth-regulating imprinted genes in deceased and surviving cloned pigs. Cloning and Stem Cells 9:97-106. Jiang, L., L. Lai, M. Samuel, R.S. Prather, X. Yang, X.C. Tian. 2008. Expression of X-linked genes in deceased neonates and surviving cloned female pigs. Mol. Reprod. Dev. 75:265-273. Klassen, H., K. Warfvinge, P.H. Schwartz, J.F. Kiilgaard, N. Shamie, C. Zhang, M. Samuel, E. Scherfig, R.S. Prather, M.J. Young. 2008. Isolation of multiple progenitor cell types from GFP-transgenic pigs and transplantation to the retina of allorecipients. Cloning & Stem Cells (in press). Li, R., K. Whitworth, L. Lai, D. Wax, L. Spate, C.N. Murphy, A. Rieke, S.C. Isom, Y. Hao, Z. Zhong, M. Katayama, H. Schatten, R.S. Prather. 2007. Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine parthenogenetic and IVF embryos. Mol. Reprod. Dev. 74:1228-1235. Liu, Z., T. Jiangtian, Z. Zhong, W. Zhenkun, S. Jun, Y. Zhi, G. Li, M. Haikun, K. Qingran, S. Shuang, L. Yutian, W. Hongbin, R.S. Prather. 2008. Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer. Chinese Science Bulletin. 53:1035-1039. Lorson, M.A., L. Spate, K. Wells, R.S. Prather, C.L. Lorson. 2008. The identification and characterization of the porcine (sus scrofa) survival motor neuron (SMN1) gene. Developmental Dynamics (in press). Lovercamp, KW, TJ Safranski, KA Fischer, G Manandhar, M Sutovsky, WO Herring and P Sutovsky. 2007. High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Archives of Andrology, 53: 219-228. Lovercamp KW, Safranski TJ, Fischer KA, Manandhar G, Sutovsky M, Herring W, Sutovsky P. 2007. Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology, Theriogenology, 67: 704-718. Martin, L.M., C. Besch-Williford, L. Lai, H.-T. Cheong, G.-S. Im, K.-W. Park, C. Murphy, D. Keisler, M. Ellersieck, H. Schatten, J.A. Green, R.S. Prather. 2007. Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos. Mol. Reprod. Dev. 74:952-960. McHughes, C.E., G.K. Springer, L. Spate, R. Li, R. Woods, M. Green, S.W. Korte, C.N. Murphy, J.A. Green, R.S. Prather. 2008. Identification and quantification of differentially represented transcripts in preimplantation bovine embryos. Mol. Reprod. Dev. (accepted 3-13-08). Rogers, C.S., Y. Hao, T. Rokhlina, M. Samuel, D.A. Stoltz, Y. Li, E. Petroff, D.W. Vermeer, A.C. Kabel, Z. Yan, L. Spate, D. Wax, C.N. Murphy, A. Rieke, K. Whitworth, M.L. Linville, S.W. Korte, J.F. Engelhardt, M.J. Welsh, R.S. Prather. 2008. Production of CFTR null and ?F508 heterozygous pigs by AAV-mediated gene targeting and somatic cell nuclear transfer. Journal of Clinical Investigation 118:1571-1577. Ross, J.W., M.D. Ashworth, F.J. White, G.A. Johnson, P.J. Ayoubi, U. E. DeSilva, K.M. Whitworth, J.A. Green, R.S. Prather, R.D. Geisert. 2007. Identification and analysis of molecular markers following endocrine disruption of pregnancy in the pig. Endocrinology 148:4761-4773. Wu, A.C.H., P. Sutovsky, G. Manandhar, W. Xu, M. Katayama, B.N. Day, Q.-W. Park, R.S. Prather, R. Oko. 2007. PAWP, A sperm specific WW-domain binding protein involved in pronuclear development during mammalian fertilization. Journal of Biological Chemistry 282:12,164-12,175. Yi, Y.J., G. Manandhar, M. Sutovsky, R. Li, V. Jonakova, R. Oko, C.S. Park, R.S. Prather, P. Sutovsky. 2007. Ubiquitin C-Terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense. Biol. Reprod. 77:780-793. Zhong, Z., L. Spate, Y. Hao, R. Li, L. Lai, M. Katayama, D. Wax, K Whitworth, Q.-Y. Sun, R.S. Prather, H. Schatten. 2007. Remodeling of centrosomes in intraspecies and interspecies nuclear transfer porcine embryos. Cell Cycle 6:1510-1521. Baltes-Breitwisch, M.M., R.C. Bott, D.M. Larson, J.M. Martin, and A.S. Cupp. 2008. Male biostimulation prior to a short melengesterol acetate (MGA) synchronization protocol increased timed AI conception rates in multiparous cows. J. Anim. Sci. (Submitted). Bass, B.E., R.A. Cederberg, G.A. Mills, C.M. Clay, and B.R. White. 2008. Responsiveness of GnRH receptor and gonadotropin subunit genes to GnRH in lines of swine divergent for ovulation rate. Biol. Reprod. (Submitted). Bott, R.C., D.T. Clopton, and A.S. Cupp. 2008. A proposed role for VEGF isoforms in sex-specific vasculature development in the gonad. Reprod. Dom. Anim. (In Press). Bott, R.C., D.T. Clopton, R.M. McFee, and AS Cupp. 2008. Kinase Domain Receptor (Kdr) expression is present in sex specific vascular patterns in the developing gonads: Role for VEGF in regulation of testis specific vasculature and spermatogenesis. Mol. Reprod. Dev. (Submitted). Cederberg, R.A., E.A. McDonald, C. Lee, R.A. Friedrich, A.R. Cropp, and B.R. White. 2008. Activity of the porcine GnRH receptor gene in aT3-1 cells is partially conferred by a distal swine upstream promoter enhancing region (SUPER) and two proximal steroidogenic factor-1 binding sites. Endocrinology (Submitted). Cherrington, B.D., T.A. Farmerie, B.E. Bass, R.A. Cederberg, C.M. Clay, and B.R. White. 2008. Emergence of a new regulatory element in the proximal promoter of the gonadotropin releasing hormone receptor in Chinese Meishan swine. Biol. Reprod. (Accepted). Harris, H.L., A.S. Cupp, A.J. Roberts, and R.N. Funston. 2007. Altered reproductive response in beef heifers fed soybeans during pubertal development. J. Anim. Sci. (Accepted). Martin, J.L., A.S. Cupp, R.J. Rasby, Z.C. Hall, and R.N. Funston. 2007. Utilization of dried distillers grains for developing beef heifers. J. Anim. Sci. 85:2298-2303. Martin, J.L., D.T. Clopton, H.L. Stroh, A.S. Cupp, and R.N. Funston. 2008. Effects of soybeans or dried distillers grains in beef heifer development diets on reproductive performance and granulosa VEGF isoform expression. J. Anim. Sci. (Submitted). McDonald, E.A., R.A. Cederberg, J.E. Smith, and B.R. White. 2008. Divergent activity of the porcine GnRH receptor gene promoter in aT3-1 cells is partially conferred by nuclear factor (NF)-kB, specificity protein (Sp)1-like, and GATA4 elements. Endocrinology (Submitted). Montagner, M.M, A.R. Cropp, J.J. Swanson, R.A. Cederberg, Paulo B.D. Goncalves, and B.R. White. 2008. Role of gonadotropin-releasing hormone on mouse preimplantation embryonic development. Mol. Reprod. Dev. (In Preparation). Pohlmann, R.M., R.A. Ten Broeck, R.M. McFee, D.T. Clopton, R.C. Bott, R.A. Longfellow, T.G. Rozell, and A.S. Cupp. 2008. Inhibition of vascular endothelial growth factor (VEGF) signal transduction blocks follicle progression to the pre-antral stage but does not necessarily affect vascular development in perinatal rat ovaries. Endocrinology (Submitted). Silva, C., J.R. Wood, L. Salvador, I. Kostetskii, C.J. Williams, and J.F. Strauss III. 2008. Expression profile of male germ cell-associated genes in mouse embryonic stem cell cultures treated with all-trans retinoic acid and testosterone. Mol. Reprod. Dev. (In Press). Ten Broeck, R.A., R.M. Pohlmann, R.A. Longfellow, M.M. Baltes, D.T. Clopton, and A.S. Cupp. 2008. Neutralization of Vascular Endothelial Growth Factor inhibitory isoforms is a more effective mechanism than treatment with angiogenic isoforms to stimulate vascular development and follicle progression in the perinatal rat ovary. Endocrinology (Submitted). Wang, H., M. Ogawa, J.R. Wood, M.S. Bartolomei, M.D. Sammel, J.P. Kusanovic, S.W. Walsh, R. Romero, and J.F. Strauss III. 2008. Genetic and epigenetic mechanisms combine to control MMP1 expression and its association with preterm premature rupture of membranes. Hum. Mol. Genet. 17:1087-1096. Wood, J.R., D.A. Dumesic, D.H. Abbott, and J.F. Strauss III. 2007. Molecular abnormalities in oocytes from women with polycystic ovary syndrome revealed by microarray analysis. J. Clin. Endocrinol. Metab, 92:705-713. Zhang, Z., R. Jaimez, X. Shen, D. Gude, H. Tang, J.R. Wood, E. Goldberg, and J.F. Strauss III. 2008. Autoregulation of SPAG16 expression: A single gene encoding an axoneme structural protein and a transcription factor that activates the axoneme protein promoter. Nucleic Acids Res. (Submitted). Albin DM, Wubben JE, Rowlett JM, Tappenden KA, Nowak RA. 2007. Small intestinal transport and barrier function respond differently to acute immune challenge in two porcine breeds. Journal of Animal Science, 85:2517-2523. Bahr JM 2008 Are scientific societies serving the needs of graduate and postdoctoral students. Reproduction, Fertility and Development 20:19-22. Bahr JM. The chicken as a model organism. In: Source Book of Models for Biomedical Research, M. Conn (ed). Humana Press, pp. 161-167, 2008. Barua A, Abramowicz J, Bahr JM, Bitterman P, Dirks A, Holub KA, Bradaric MJ, Edassery SL, and Luborsky JL. 2007. Detection of ovarian tumors in chicken by ultrasonography: a step towards early diagnosis in humans? J of Ultrasound and Medicine 26:909-919. Barua A, Edassery SL, Bitterman P, Abramowicz JS, Dirks AL, Bahr JM, Hales DB, Bradaric MJ, Luborsky JL 2008 Prevalence of anti-tumor antibodies in laying hen model of human ovarian cancer. International J. of Gynecol. Cancer. (in press). Belton Jr. RB, Chen L, Mesquita FS, Nowak RA. 2008. Basigin-2 is a cell surface receptor for soluble basigin ligand. J Biol Chem [Epub April 22, 2008]. Bleck GT, MB Wheeler, LB Hansen, H Chester-Jones, and DJ Miller. 2008. Lactose synthase components in milk: Levels of a-lactalbumin and b1,4galactosyltransferase in milk of cows from several breeds at various stages of lactation. Reprod Domestic Animals. In Press. Boltz CR, Boltz DA, Bunick D, Scherba G, Bahr JM. 2007. Vaccination against the avian infectious bronchitis virus affects sperm concentration, sperm quality, and blood testosterone concentrations in cockerels. British Poultry Science 48;617-624. Braundmeier AG, WG Breed, and DJ Miller. 2008. Spermatozoa from a marsupisal, the Brushtail Possum, contain b1,4-galactosyltransferase. Reprod Fertilit Develop. 20:402-407. Chen L, Nakai M, Belton Jr. R J, and Nowak RA. 2007. Expression of extracellular matrix metalloproteinase inducer and matrix metalloproteinases during mouse embryonic development. Reproduction 133:405-414. Collins ED, WL Flowers, RD Shanks, and DJ Miller. 2008. Porcine sperm-zona binding ability as an indicator of fertility. Anim Reprod Sci. 104:69-82. Hales DB, Zhuge Y, Lagman JAJ, Ansenberger K, Mahon C, Barua A, Luborsky J, Bahr JM 2008 Cyclooxygenases expression and distribution in the normal ovary and their role in ovarian cancer in the domestic hen (Gallus Domesticus). Endocrine (in press). Herrick, J.R., Bond, J.B., Magarey, G.M., Bateman, H.L., Krisher, R.L., Dunford, S.A., Swanson, W.F. (2007) Toward a feline-optimized culture medium: impact of ions, carbohydrates, essential amino acids, vitamins, and serum on development and metabolism of in iitro fertilization-derived feline embryos relative to embryos grown in vivo. Biol. Reprod. 76:858-870. Krisher, R.L., Brad, A.M., Herrick, J.R., Sparman, M.L., Swain, J.E. (2007) A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation. Animal Reproduction Science. 98:72-96. Landers EA, HR Burkin, G Bleck, L Lesley Howell-Skalla, and DJ Miller. 2008. Porcine b1,4galactosyltransferase-I sequence, mapping, and expression. Reprod Domestic Animals. In Press. Miles, J.R., Blomberg, L.A., Krisher, R.L., Everts, R.E., Sonstegard, T.S., Van Tassell, C.P., Zuelke, K.A. (2008) Comparative transcriptome analysis of in vivo- and in vitro-produced porcine blastocysts by small amplified RNA-serial analysis of gene expression (SAR-SAGE). Molecular Reproduction and Development. 75:976-988. Paris, M.C.J., Mastromonaco, G.F., Paris, D.B.B.P., Krisher, R.L. (2007) A perspective on the role of emerging technologies for the propogation of companion animals, non-domestic and endangered species. Reproduction, Fertility and Development 19(6), iii-vii. Stammer K, Edessary SL, Barua A, Bitterman P, Bahr JM, Hales DB, Luborsky JL 2008 Selenium binding protein 1 expression in ovaries and ovarian tumors in the laying hen, a spontaneous model of human ovarian cancer. Gynecologic Oncology 109(Apr):115-121. Stevenson, J.S., Tenhouse, D.E., Krisher, R.L., Lamb, G.C., Larson, J.E., Dahlen, C.R., Pursley, J.R., Bello, N.M., Fricke, P.M., Wiltbank, M.C., Brusveen, D.J., Burkhart, M., Youngquist, R.S., Garverick, H.A. 2008. Detection of anovulation by heatmount detectors and transrectal ultrasonography before treatment with progesterone in a timed insemination protocol. Journal of Dairy Science. (Accepted) Wheeler, M.B. 2007. Agricultural applications for transgenic livestock. Trends in Biotechnology 25:204- 210. Wheeler, M.B. Lima, A.S., VanEtten, T., Mello, M.R.B., 2007. Transgenic animals for biomedicine and agriculture. In, Bovine Theriogenology, de la Sota, R.L. and Bo, G.A., Eds. 36 pgs. (in press). Wheeler, M.B., D. Kim, E. Monaco, W.L. Hurley 2007. Application of robotic and microfluidic technologies to stem cell culture and analysis. Tsukuba Meeting for Animal Biotechnology (TMAB)  Progress in Somatic Cell Nuclear Transfer Technology, January 12-12, 2007 Tsukuba City, Japan, Pgs. 31-32. Wheeler, M.B., Davidson, T.R., Malusky, S.A., Ferguson, C.E., Lima, A.S., Mello, M. B. 2007. Production of transgenic livestock: Technology and applications. In: Biotécnicas Aplicadas à Reprodução Animal, eds. P.B. Goncalves & J. Figuriedo, (new edition in press). Wheeler, M.B., Mello, M.R.B., Lima, A.S., VanEtten, T. 2007. Modification of the domestic animal genome. In, Bovine Theriogenology, de la Sota, R.L. and Bo, G.A., Eds., 45 pgs. (in press). Wheeler, M.B., Walters, E.M. Beebe, D. J. 2007. Toward culture of single gametes: The development of microfluidic platforms for assisted reproduction. Theriogenology 68S:S178-S189. Woodard, J., A. Hilldore, , M. B. Wheeler, S. Clark, S. Lan, R. Jamison, and A. J. Wagoner Johnson, 2007. The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28:4554. Zhao H, K Reim, N Brose, and DJ Miller. 2008. Complexin I deficient sperm are subfertile due to defects in the acrosome reaction. Submitted. Zhao L*, H Burkin*, X Shi, L Li, K Reim, and DJ Miller. 2007. Complexin I is required for mammalian sperm acrosomal exocytosis. (*Both authors contributed equally to the paper). Dev Biol 309:236-244. Zhao L, X Shi, L Li, and DJ Miller. 2007. Dynamin 2 associates with complexins and is found in the acrosomal region of mammalian sperm (cover feature). Mol Reprod Dev 74:750-757. Cassar, G., R.N. Kirkwood, M.J. Seguin, T. Widowski, A. Farzan, A.J. Zanella and R.M. Friendship. 2008. Influence of stage of gestation at grouping and presence of boars on farrowing rate and litter size of group-housed sows. J. Swine Health Prod. 16:81-85. Straw, B.E., R.O. Bates and G. May. 2008. Influence of method of administration of prostaglandin on farrowing and relationship between gestation length and piglet performance. J. Swine Health Prod. 16:138-143. Zak, L.J., G.R. Foxcroft, F.X. Aherne and R.N. Kirkwood. 2008. Role of luteinizing hormone in primiparous sow responses to split weaning. Reprod. Dom. Anim. (in press). Manjarin, R., G. Cassar, D.J. Sprecher, R.M. Friendship, J.C. Dominguez and R.N. Kirkwood. 2008. Effect of eCG or eCG plus hCG on estrus expression and ovulation in prepubertal gilts. Reprod. Dom. Anim. (in press). Manjarin, R., J.C. Dominguez, M.J. Castro, B. Vallado, S. Rodriguez, D.J. Sprecher, G. Cassar, R.M. Friendship and R.N. Kirkwood. Effect of prior FSH treatment on the estrus and ovulation responses to eCG in prepubertal gilts. Anim. Reprod. Sci. (in press). Manjarin, R., J.C. Dominguez, M.J. Castro, D.J. Sprecher, G. Cassar, R.M. Friendship and R.N. Kirkwood. 2008. Effect of hCG treatment on the estrus and ovulation responses to FSH in prepubertal gilts. Reprod. Dom. Anim. (in press). Pelland, C, G. Cassar, R.N. Kirkwood and R.M. Friendship. 2008. Fertility following intrauterine insemination with a reduced number of spermatozoa in sows with synchronized ovulation. J. Swine Health Prod. (in press). Cassar G, R.N. Kirkwood and R.M. Friendship. Low sow reproductive performance related to estrus detection and timing of insemination. The Pig Journal (in press). Lee, K, Fodor, WL, Machaty, Z. 2007. Dynamics of lamin A/C in porcine nuclear transfer embryos. Mol. Reprod. Dev. 74:1221-1227. Magnani, L, Cabot, R. 2007. Developmental arrest is induced when the chromatin remodeling protein SMARCA2 in overexpressed in cleavage stage porcine embryos. Molecular Reproduction and Development 74(10), pages 1262-1272. Magnani, L, Cabot, R. (In Press) In vitro and in vivo derived porcine embryos possess similar, but not identical, patterns of Oct4, Nanog and Sox2 mRNA expression during cleavage development. Molecular Reproduction and Development. Magnani, L, Lee, K, Fodor, W, Machaty, Z, Cabot, R. 2007. Developmental capacity of porcine nuclear transfer embryos correlate with levels of chromatin remodeling transcripts in donor cells. Molecular Reproduction and Development 75(5):766-76. Nanassy, L, Lee, K, Javor, A, Machaty, Z. 2008. Effects of activation methods and culture conditions on development of parthenogenetic porcine embryos. Anim. Reprod. Sci. 104:264274. Nanassy, L, Javor, A, Machaty, Z. 2007. Changes in MPF and MAPK activities in porcine oocytes activated by different methods. 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