Brief Summary of Minutes of Annual Meeting

The annual meeting of the NCERA-57 was called to order by Mark Diekman, local host, at 8:15 AM on Wednesday June 25th. Mark introduced Chuck Hibberd, Associate Dean & Director of Cooperative Extension Service. Chuck welcomed us to campus and gave us a brief update on the Purdue University Extension Service and how he was working towards a focus on Transformation Extension Programs. The goal is to raise the bar from level 1 one-on-one contacts to level 4 transformational extension programs.

Mark then introduced Alan Grant, Department Head of Animal Science. Alan spoke about recent trends in animal agriculture in Indiana and the Governors goals for increasing animal agriculture in Indiana. He indicated that in the face of a 30% decrease in pig numbers in the last 17 years, the number of feeder pigs had increased 1000% indicating a shift in the nature of the swine industry in the state. He also indicated that over the same time period the undergraduate enrollment in Animal Science had increased from ~200 in 1990 to ~600 undergraduate students in 2008. Discussion ensued about the demographics and interests of those students and that similar trends have been observed at other stations.

John Baker, Associate Director of the Michigan Agricultural Experiment Station and Administrative Advisor to NCERA-57, addressed the need to begin the process of rewriting the NCERA-57 project. He indicated that he could give editorial rights to three individuals. We codified the previously discussed concept that John Parrish would chair the rewrite committee and that Matt Wilson and Dave Guthrie would fill out the committee. John distributed a handout containing pertinent information for the rewrite effort and the deadlines for the various components of the renewal. He emphasized the need for impact statements and commended our project for their success, including the submission and planned resubmission of an integrated grant proposal to USDA- NRICGP and the biennial symposium.

Station reports then began. Duane Davis spoke about the online lab class that he has been developing at Kansas State to replace their wet lab for online students. He indicated that aspects of this would fit into the teaching component of the integrated project resubmission. One of his significant questions was how to best use modules for on and off campus. Station reports continued until about 11 at which time we travelled to JBS United in Sheridan, IN for the symposium organized by the regional project on Sow Longevity.

The business meeting was called to order on June 26th. David Miller was unanimously elected the next Secretary for NCERA-57. West Virginia University was selected as the host institution for the next annual meeting and dates were discussed. If acceptable, the group settled on the 24th and 25th of June, 2009 with June 23rd serving as a travel day.

Plans for the next symposium were initiated and a tentative topic of gilt development from prenatal to mating was arrived at. John Parrish asked that all members send him brief impact statements that he could use to initiate the renewal process. Finally, the regional committee thanked Mark Diekman and Purdue University for graciously hosting the annual meeting and letters of thanks will be sent to Mark and to JBS United for hosting and supporting the biennial symposium.

Dave Guthrie spoke briefly about the grant resubmission. He indicated that the two main issues were the proposed dose of the synchronization compound and the need for teaching and economic analyses. He also indicated that he would again be soliciting letters of support.

Station reports then resumed with reports from Bret White, Lloyd Anderson, Tim Safranski, John Parrish, Joe Ford and Rebecca Krisher.

The meeting was adjourned at 11:00 and the gavel was virtually passed from John Parrish to Dave Guthrie.

Respectfully submitted Matthew Wilson, Secretary, NCERA-57

1. The NCERA-57 committe, with leadership from Dave Guthrie and Duane Davis, revised the integrated proposal entitled Seasonal Infertility in Swine: Research and Educational Goals to Enhance the Profitability and Sustainability of the Swine Industry that was not funded in 2007, unfortunately it was not submitted in time for the 2007 grant cycle. Work continues to proceed relative to additional refinement of the proposal and it should be resubmitted in 2008. 2. The committee organized and hosted its biennial symposium entitled Sow Longevity. The focus of the symposium was to extend the efforts of the committee and others to extension faculty, producers and individuals affiliated with the swine industry. Greater that 60 attendees pack the conference room at JBS United for what was an excellent series of presentations and discussion. The slides presented will be put together on to CDs that will be distributed to the committee, extension agents and interested individuals. The presentations should also be available via the website that John Parrish continues to develop to serve as a repository of information and tools for and from the committee. 3. A topic was identified for the next symposium that will focus on animal management from the prenatal period through breeding.

Sagirkaya H, Misirlioglu M, Kaya A, First NL, Parrish JJ, Memili E. Developmental potential of bovine oocytes cultured in different maturation and culture conditions. Anim Reprod Sci. 2007 Oct;101(3-4):225-40. Jerde TJ, Mellon WS, Bjorling DE, Checura CM, Owusu-Ofori K, Parrish JJ, Nakada SY. Stretch induction of cyclooxygenase-2 expression in human urothelial cells is calcium- and protein kinase C zeta-dependent. Mol Pharmacol. 2008 Jan;73(1):18-26. Siddiqui MA, Bhattacharjee J, Das ZC, Islam MM, Islam MA, Haque MA, Parrish JJ, Shamsuddin M. Crossbred bull selection for bigger scrotum and shorter age at puberty with potentials for better quality semen. Reprod Domest Anim. 2008 Feb;43(1):74-9. K.L. Willenburg, K.J. Rozenboom and J.J. Parrish. The relationship between sperm nuclear shape and boar fertility using fourier harmonics. Journal of Animal Science, Volume 86, E-Supplement 2, 2008. p 525. J. A. Sterle and J. J. Parrish. Changes that have occurred in animal science teaching. Journal of Animal Science, 2008. Volume 86, E-Supplement 2, p 345. J. J. Parrish. The use of podcasts in the classroom. Journal of Animal Science, Volume 86, E-Supplement 2, 2008. p 346. J.J. Parrish, C. Mueller, E. Ludwig and J. L. Susko-Parrish. Day length does not affect live sperm nuclear shape in the stallion. Reproduction, Fertility and Development. 2008 20:192. Lefric Enwall and John Parrish. Effect of scrotal insulation on bull sperm in vitro fertility, nuclear shape and apoptotic markers. Journal of Andrology Supplement, Proceedings of the American Society of Andrology 33rd Annual meeting. 2008. p 47. Antelman, J., G. Manandhar, Y.-J. Yi, R. Li, K. Whitworth, J.A. Green, R.S. Prather, P. Sutovsky. 2008. Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development. J. Cellular Physiology. (accepted 6-3-08). Bonk, A.J., H.-T. Cheong, R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason, K.M. Whitworth, C.N. 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Cloning & Stem Cells (in press). Li, R., K. Whitworth, L. Lai, D. Wax, L. Spate, C.N. Murphy, A. Rieke, S.C. Isom, Y. Hao, Z. Zhong, M. Katayama, H. Schatten, R.S. Prather. 2007. Concentration and composition of free amino acids and osmolalities of porcine oviductal and uterine fluid and their effects on development of porcine parthenogenetic and IVF embryos. Mol. Reprod. Dev. 74:1228-1235. Liu, Z., T. Jiangtian, Z. Zhong, W. Zhenkun, S. Jun, Y. Zhi, G. Li, M. Haikun, K. Qingran, S. Shuang, L. Yutian, W. Hongbin, R.S. Prather. 2008. Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer. Chinese Science Bulletin. 53:1035-1039. Lorson, M.A., L. Spate, K. Wells, R.S. Prather, C.L. Lorson. 2008. The identification and characterization of the porcine (sus scrofa) survival motor neuron (SMN1) gene. Developmental Dynamics (in press). Lovercamp, KW, TJ Safranski, KA Fischer, G Manandhar, M Sutovsky, WO Herring and P Sutovsky. 2007. High resolution light microscopic evaluation of boar semen quality sperm cytoplasmic droplet retention in relationship with boar fertility parameters. Archives of Andrology, 53: 219-228. Lovercamp KW, Safranski TJ, Fischer KA, Manandhar G, Sutovsky M, Herring W, Sutovsky P. 2007. Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in boars. Theriogenology, Theriogenology, 67: 704-718. Martin, L.M., C. Besch-Williford, L. Lai, H.-T. Cheong, G.-S. Im, K.-W. Park, C. Murphy, D. Keisler, M. Ellersieck, H. Schatten, J.A. Green, R.S. Prather. 2007. Morphologic and histologic comparisons between in vivo and nuclear transfer derived porcine embryos. Mol. Reprod. Dev. 74:952-960. McHughes, C.E., G.K. Springer, L. Spate, R. Li, R. Woods, M. Green, S.W. Korte, C.N. Murphy, J.A. Green, R.S. Prather. 2008. Identification and quantification of differentially represented transcripts in preimplantation bovine embryos. Mol. Reprod. Dev. (accepted 3-13-08). Rogers, C.S., Y. Hao, T. Rokhlina, M. Samuel, D.A. Stoltz, Y. Li, E. Petroff, D.W. Vermeer, A.C. Kabel, Z. Yan, L. Spate, D. Wax, C.N. Murphy, A. Rieke, K. Whitworth, M.L. Linville, S.W. Korte, J.F. Engelhardt, M.J. Welsh, R.S. Prather. 2008. Production of CFTR null and ?F508 heterozygous pigs by AAV-mediated gene targeting and somatic cell nuclear transfer. Journal of Clinical Investigation 118:1571-1577. Ross, J.W., M.D. Ashworth, F.J. White, G.A. Johnson, P.J. Ayoubi, U. E. DeSilva, K.M. Whitworth, J.A. Green, R.S. Prather, R.D. Geisert. 2007. Identification and analysis of molecular markers following endocrine disruption of pregnancy in the pig. Endocrinology 148:4761-4773. Wu, A.C.H., P. Sutovsky, G. Manandhar, W. Xu, M. Katayama, B.N. Day, Q.-W. Park, R.S. Prather, R. Oko. 2007. PAWP, A sperm specific WW-domain binding protein involved in pronuclear development during mammalian fertilization. Journal of Biological Chemistry 282:12,164-12,175. Yi, Y.J., G. Manandhar, M. Sutovsky, R. Li, V. Jonakova, R. 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A proposed role for VEGF isoforms in sex-specific vasculature development in the gonad. Reprod. Dom. Anim. (In Press). Bott, R.C., D.T. Clopton, R.M. McFee, and AS Cupp. 2008. Kinase Domain Receptor (Kdr) expression is present in sex specific vascular patterns in the developing gonads: Role for VEGF in regulation of testis specific vasculature and spermatogenesis. Mol. Reprod. Dev. (Submitted). Cederberg, R.A., E.A. McDonald, C. Lee, R.A. Friedrich, A.R. Cropp, and B.R. White. 2008. Activity of the porcine GnRH receptor gene in aT3-1 cells is partially conferred by a distal swine upstream promoter enhancing region (SUPER) and two proximal steroidogenic factor-1 binding sites. Endocrinology (Submitted). Cherrington, B.D., T.A. Farmerie, B.E. Bass, R.A. Cederberg, C.M. Clay, and B.R. White. 2008. Emergence of a new regulatory element in the proximal promoter of the gonadotropin releasing hormone receptor in Chinese Meishan swine. Biol. Reprod. (Accepted). Harris, H.L., A.S. Cupp, A.J. Roberts, and R.N. Funston. 2007. Altered reproductive response in beef heifers fed soybeans during pubertal development. J. Anim. Sci. (Accepted). Martin, J.L., A.S. Cupp, R.J. Rasby, Z.C. Hall, and R.N. Funston. 2007. Utilization of dried distillers grains for developing beef heifers. J. Anim. Sci. 85:2298-2303. Martin, J.L., D.T. Clopton, H.L. Stroh, A.S. Cupp, and R.N. Funston. 2008. Effects of soybeans or dried distillers grains in beef heifer development diets on reproductive performance and granulosa VEGF isoform expression. J. Anim. Sci. (Submitted). McDonald, E.A., R.A. Cederberg, J.E. Smith, and B.R. White. 2008. Divergent activity of the porcine GnRH receptor gene promoter in aT3-1 cells is partially conferred by nuclear factor (NF)-kB, specificity protein (Sp)1-like, and GATA4 elements. Endocrinology (Submitted). Montagner, M.M, A.R. Cropp, J.J. Swanson, R.A. Cederberg, Paulo B.D. Goncalves, and B.R. White. 2008. Role of gonadotropin-releasing hormone on mouse preimplantation embryonic development. Mol. Reprod. Dev. (In Preparation). Pohlmann, R.M., R.A. Ten Broeck, R.M. McFee, D.T. Clopton, R.C. Bott, R.A. Longfellow, T.G. Rozell, and A.S. Cupp. 2008. Inhibition of vascular endothelial growth factor (VEGF) signal transduction blocks follicle progression to the pre-antral stage but does not necessarily affect vascular development in perinatal rat ovaries. Endocrinology (Submitted). Silva, C., J.R. Wood, L. Salvador, I. Kostetskii, C.J. Williams, and J.F. Strauss III. 2008. Expression profile of male germ cell-associated genes in mouse embryonic stem cell cultures treated with all-trans retinoic acid and testosterone. Mol. Reprod. Dev. (In Press). Ten Broeck, R.A., R.M. Pohlmann, R.A. Longfellow, M.M. Baltes, D.T. Clopton, and A.S. Cupp. 2008. 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