SAES-422 Multistate Research Activity Accomplishments Report

Status: Approved

Basic Information

Participants

C. Kaltenbach AZ W-173 Administrative Advisor D. Hamernick USDA-CSREES USDA-CSREES Representative A. Parkhurst NE W-173 2002-2003 Chair J. Arthington Fl W-173 2002-2003 Secretary H. Cheng USDA-ARS B. Salfen USDA-ARS T. Strauch USDA-ARS T. Mader NE J. Carroll USDA-ARS T. Brandl USDA-MARC P. Hillman NY C. Lee HI H. Lin NE J. Wu NE D. Spiers MO J. Davis KY J. Bicudo KY H. Kattesh TN J. Nienaber USDA-MARC E. Antoniou MO T. Walsh TX S. Bhusari MO E. Minton KS S. Eicher USDA-ARS

The meeting began at 8:30 on August 8th with self-introductions by the meeting participants. C. Kaltenbach brought the group up-to-date on the administrative status of the project. D. Hamernick (USDA-CSREES) then made a presentation on current USDA activities. Presentations of the W-173 members were made to apprise participants of the current research and multi-state collaborative activities. At 6:30pm the W-173 business meeting was called to order. The previous years minutes were approved. J. Bicudo (KY) was named secretary and J. Arthington (FL) Chair of W-173 for 2003-2004. A decision was made to meet next year in Orlando. J. Arthington will investigate opportunities to hold the meeting on the Walt Disney property during July 13 and 14 or 20 and 21. On August 9th a tour of the Henry Doorley Zoo was conducted at 8:30. The group met with Douglas Armstrong, DVM, senior veterinarian to discuss the shelter and well-being of the zoo animals. The meeting adjourned at 12:00 pm on August 9th

Accomplishments

Note that the principal participants are indicated in relation to the stated accomplishment and subsequent impact. However, in almost all cases these are multi-state collaborative projects that involve one or more W-173 participants.

Objective I: Identify appropriate measures of animal stress and well-being and
characterize factors affecting the biology of the stress response

*Accomplishment: NE-USDA-MARC Results of a study of relative heat tolerance among cattle of different genetics indicated dark-hided cattle were more stressed than light-hided cattle. Heat stress more than doubled drinking behavior, increased standing, and decreased eating, lying, and agonistic behaviors. Impact: It was found that dark-hided cattle with higher condition scores were more adversely affected by heat stress.

*Accomplishment: TN Studies showed the free cortisol index can be used as a surrogate marker for plasma free cortisol in the pigs since it correlates well with plasma free cortisol, which is the biologically active component. Impact: The free cortisol index provides a simple, less time consuming assessment of situations resulting in significant changes in plasma total cortisol levels by taking into account shifts in CBG concentration.

*Accomplishment: NE The bi-logistic model followed the dynamics of the handling process. The predictions for time to reach maximum body temperature were closer to the observed value than either the or hormetic estimates and the prediction of maximum body temperature was better than the bi-logistic and as good as the hormetic estimate. Moreover, the segmented model was robust to different definitions of recovery time. Impact: Results of this work will improve our ability to characterize thermal stress in livestock and enable us to compare breeds, management strategies and to identify cattle that are resistant or sensitive to thermal stress.

*Accomplishment: AL and KS collaborated on a study designed to evaluate gene expression in various immune and endocrine tissues at 6 and 24 hr after i.p. LPS injection. Tissues were collected at sacrifice and total RNA was later extracted for evaluation utilizing differential display PCR. Differential display procedures are currently in progress. Additionally, KS evaluated expression of toll-like receptors (TLR) and chemokines in cultured porcine jejunal epithelial cells infected with Salmonella serovars. Salmonella infection increased TLR-2 and ?5, as well as CCL-20 and IL-8. Impact: Results suggest the gastrointestinal epithelium is an important regulator of immune cell function.

*Accomplishment: AZ Heat shock proteins (HSP) were assessed by western blot analysis in semen samples from normal and cryptorchid stallions. HSP?s were not detected in any semen samples indicating that ejaculated sperm do not express HSP?s. Samples to testicular tissue from normal and crytorchid stallions were then assessed for HSP profiles. HSP40 and HSP70 were detected in both normal and retained testes. However, HSP 70 expression levels were 2-fold higher in retained than normal testes tissue. Impact: Results suggest a possible association of HSP70 in sperm from abdominally retained (heated) stallion testes.

*Accomplishment: MO-USDA Investigated the immunological and physiological responses of young pigs to a live bacterial challenge by determining the acute immune response in pigs challenged with E. coli. Impact: An understanding of the immunological and physiological responses associated with live bacterial challenges is necessary before novel approaches for preventing E. coli outbreaks in swine herds can be effectively implemented.

*Accomplishment: MO conducted an experiment to determine the effect of successively higher levels of heat challenge on thermal status of cattle. Hysteresis loops for Tcore showed distinct time-of-day differences in response to air temperature change. These loops were different for low and high heat stress periods. Likewise, these loops showed downward shifts during the first week of adaptation, with little change after this time. Impact: These results show that hysteresis loops can be used to characterize core body temperature response of cattle to heat stress in the field. It appears that cattle adapt to heat in this environment by delaying the change in core body temperature with the daily shift in air temperature.


Objective II: Evaluate management strategies that minimize the detrimental effects
of animal stress

*Accomplishment: UF completed additional studies aimed at characterizing the influence of copper nutrition on the health and productivity of beef cattle. Impact: These findings indicate that copper nutrition is an important contributor to early responses to inflammatory signals. Applications for improving copper nutrition will likely increase the effectiveness of vaccines containing adjuvant carriers.

*Accomplishment: UF completed a transportation stress/feedlot receiving study to investigate the effects of early calf weaning on the stressors associated with weaning and transportation. Impact: These findings indicate that early calf weaning has a significant effect on lessening physiological characteristics associated with the stress of calf weaning. This effect translated into nearly a 25% improvement in feed efficiency during the feedlot receiving and growing period.

*Accomplishment: NY, MS and HI tested a vaginal temperature logger for dairy cows and determined it can effectively measure body temperature. The logger is comprised of a commercial waterproof temperature logger encased in a specially constructed, soft plastic anchor with 8 fingerlike projections to keep it from being discharged from the vagina. Impact: Taking spot measurements of body temperature with a rectal thermometer cannot be done frequently enough to explore the dynamics of thermoregulation and it disrupts the behavior of the cows. The vaginal temperature logger overcomes these limitations with an easy to use, non-disruptive, low cost, continuous record of body temperature.

*Accomplishment: NY, HI and MS observed the thermoregulatory responses of heat-stressed dairy cows in a freestall barn in a hot-humid environment. When cows are laying in freestalls, their body temperatures rises under fan cooling. Spray cooling reduces the rate of this rise by one-half. When their body temperature reaches about 39°C, the cows stand up in their stalls. Upon standing cows can cool with fans alone. They cool twice as fast when fans are supplemented with feedline spray. Impact: Heat-stress in dairy cows causes great economic losses to the dairy industry as well as adversely affecting the well-being of cows, where successful management requires an understanding the dynamics thermal responses within a freestall barn.

*Accomplishment: NY, HI and AZ compared the evaporation rates of black and white heat-stressed dairy cows under simulated direct solar radiation in a hot-dry environment. Black hair coats absorb more solar radiation and are warmer and have higher sweating rates than white hair coats. Impact: Solar radiation imposes a severe thermal load on cows where the impact of black hair coats and high sweating rates must be included in management strategies.

*Accomplishment: MO-USDA assessed endocrine and tissue responses to 72 h feed deprivation in weaned pigs and also determined the effects of exogenous ghrelin on endocrine, behavioral, feed intake and weight gain responses in pigs that are undergoing the post-weaning transition to a solid diet. Impact: This work on appetite regulation as it relates to the weaning process will be valuable in the search for methods which can be utilized to prevent weigh loss associated with the naturally occurring feed deprivation during this period. Decreasing the time pigs go without feed during the postweaning period will enhance growth and performance, as well as reduce the incidence of early postweaning morbidity and mortality.

*Accomplishment: MO-USDA determined the effects of an estrogenic compound (Ralgro; R) on maternal and piglet performance and components of the somatotrophic axis. Impact: This work supports theory that prenatal and perinatal treatments can be utilized to enhance pig productivity during the postnatal period.

*Accomplishment: MO Further evaluation of the effect of fescue toxicosis on cattle performance showed that a dietary seaweed product will produce a temporary reduction in core body temperature during heat stress in both chamber and field environments. A higher level of dietary iodide is not responsible for this reduction. Impact: Seaweed-derived feed additives can be used to reduce heat strain in cattle.

*Accomplishment: KY showed air temperatures within the trailer during transport were
significantly higher than the ambient air temperatures, and varied with position in the trailer. The air temperature was lowest at the rear of the trailer, and was significantly higher at the front stall. Impact: Determining the ventilation rate within the trailer will allow for better understanding of the thermal environment horses are subjected to during transport.

*Accomplishment: KY and IA developed and tested algorithms for determining individual bird feeding statistics and stereotyped pecking behavior from time-series recordings of feed weight. Impact: By scientifically characterizing the feeding behavior of laying hens, baseline information will result that may help better quantify the welfare of birds.

*Accomplishment: NE-USDA-MARC Heat stress response of cattle to climate variables of temperature, humidity, wind speed, and solar radiation have been collected and an algorithm developed to represent animal response in the form of RR. The response was then compared to response of animals in various categories of stress: alert, danger, and emergency. Impact: This tool uses current weather measurements at the feedlot and will assist feedlot operators and cattle producers in management decisions involving heat waves.
Plans for the Coming Year-W-173 Collaborations (2003-2004)

Impacts

  1. See accomplishment section for impacts.

Publications

Arizona

Peer-reviewed:
Suchyta, S.P., S. Sipkovsky, R. Kruska, A. Jeffers, A. McNulty, R.J. Templeman, R.G.Halgren, P.M. Samma, D.E. Bauman, Y.R. Boisclair, J.L. Burton, R.J. Collier, E.J. DePeters, T.A. Ferris, M.C. Lucy, M.A. McGuire, J.F. Medrano, T.R. Overton, T.P. Smith, G.W. Smith, T.S. Sonstegard, J.N.Spain, D.E. Spiers, J.Yao and P.M. Coussens. 2003. Development and Testing of a High-Density cDNA Microarray resource for Cattle. Physiol.Genom. (In Press).

Kajimura, S., K. Uchida, T. Yada, L.G. Riley, J.C. Byatt, R.J. Collier, K. Aida, T. Hirano and E.G. Grau. 2002. Stimulation of insulin-like growth factor-I production by recombinant bovine growth hormone in Mozambique tilapia, Oreochromis mossambicus. Fish Physiol Biochem. 25:221-230.

Kelsey, J.A., B.A. Corl, R.J. Collier and D.E. Bauman. 2003. The effect of breed, parity and stage of lactation on conjugated linoleic acid (CLA) in milk fat from dairy cows. J. Dairy Sci (In Press)

Collier, R.J., Y, Kobayashi and P. Gentry. 2003. The use of genomics in genetic selection programs for environmental stress tolerance in domestic animals. Proceedings 15th Conf. Biometeorol. And Aerobiol.pp 54-58. American Meteorol. Soc. Press.

Abstracts:
Collier, R.J., E.L. Annen , D.E. Armstrong and A.L. Wolfgram. 2003. Evaluation of two evaporative cooling systems for dairy cattle under semi-arid conditions. J. Anim Sci. vol 81, Suppl 1:18 (abstract)


Florida

Peer-reviewed:
Arthington, J.D., F. Blecha, and F.G. Martin. 2003. Effect of molybdenum and sulfur feeding on the acute phase protein response to inflammatory challenge in beef heifers. Prof. Anim. Sci. 19:221-226.

Arthington, J.D., F.M. Pate, and J.W. Spears. 2003. Effect of copper source and level on performance and copper status of cattle consuming molasses supplements. J. Anim. Sci. 81:1357-1362.

Arthington, J.D., and R.S. Kalmbacher. 2003. Effect of early weaning on beef cow and calf performance in the subtropics. J. Anim. Sci. 81:1136-1141.

Arthington, J.D., S.D. Eicher, W.E. Kunkle, and F.G. Martin. 2003. Effect of transportation and commingling on the acute phase protein response, growth and feed intake of newly weaned beef calves. J. Anim. Sci. 81:1136-1141.

Abstracts:
Arthington, J.D., F.M. Pate, and J.W. Spears. 2003. Effect of copper source and level on performance and copper status of cattle consuming molasses-based supplements. J. Anim. Sci. 81(Suppl. 1):79 (abstract)

Arthington, J.D., and J.W. Spears. 2003. The effect of early calf weaning on performance and measures of stress during the feedlot receiving period. J. Anim. Sci. 81(Suppl. 1):169 (abstract).

Arthington, J.D., and R.S. Kalmbacher. 2003. The effect of early calf weaning followed by ryegrass (Lolium multiflorum Lam.) grazing on performance of fall-born calves in Florida. J. Anim. Sci. 81(Suppl. 2):1 (abstract).

Vendramini, J.M.B., J.D. Arthington, L.E. Sollenberger, R.S. Kalmbacher, and P. Mislevy. 2003. Performance of early-weaned calves grazing stargrass (Cynodon nlemfuensis) and Atra paspalum (Paspalum atratum) pastures during summer. J. Anim. Sci. 81(Suppl. 2):1 (abstract).

Arthington, J.D. 2003. Utilization of early weaning for improving the productivity of beef heifers. Proc. 7th Annual Curso Novos Enfoques Na Producao E Reproducao De Bovinos. Uberlandia, Minas Gerais, Brazil. p. 230 (conference proceedings).


Kansas State

Peer-reviewed:
Arthington, J.D., F. Martin, and F. Blecha. 2003. Effect of molybdenum and sulfur feeding on acute phase protein response to inflammatory challenge in beef heifers. Prof. Anim. Scientist. 19:221-226.

Daniel, J.A., M.S. Abrams, L. de Souza, C.G. Wagner, B.K. Whitlock, and J.L. Sartin. 2003. Endotoxin inhibition of luteinizing hormone in sheep. Dom. Anim. Endocrinol. 25:13-19.

Davis, E.G., B.R. Rush, and F. Blecha. 2003. Increases in cytokine and antimicrobial peptide gene expression in horses by immunomodulation with Propionibacterium acnes. Vet. Therapeutics. 4:5-11.

Lu, N., Y. Wang, F. Blecha, R.J. Fels, H.P. Hoch, and M.J. Kenney. 2003. Central interleukin-1 beta antibody increases renal and splenic sympathetic nerve discharge. Am. J. Physiol. Heart Circ. Physiol. 284:H1536 H1541.

Ropp J.K., R. H. Raub, and J. E. Minton. 2003. The effect of dietary energy source on serum concentration of insulin-like growth factor-I growth hormone, insulin, glucose, and fat metabolites in weanling horses. J. Anim Sci. 81:1581-1589.

Sartin, J.L., T.H. Elsasser, S. Kahl, J. Baker, J.A. Daniel, D.D. Schwartz, B. Steele, B.K. Whitlock. 2003. Estradiol and progesterone treatment modulates select elements of the proinflammatory cytokine cascade: Attenuated nitric oxide and thromboxane B2 production in endotoxemia. J. Anim. Sci. 81:1546-1551.

Turner, J. L., M.J. Arns, and J. E. Minton. 2003. Case study: Effects of non-specific immunostimulation of prepartum mares on colostral quality and foal immune function. Prof. Anim. Sci. 19: 62-67.

Turner, J. L., M.J. Arns, J. E. Minton, and J. A. Pruitt. 2003. Effect of abrupt vs gradual weaning on cortisol and immune function responses of foals. Prof. Anim. Sci. 19: 55-61.

Wu, H., C.R. Ross, and F. Blecha. 2002. Characterization of an upstream open reading frame in the 5‘‘‘‘ untranslated region of PR-39, a cathelicidin antimicrobial peptide. Mol. Immunol. 39:9-18.

Abstracts:
Burkey, T.E., K.A. Skjolaas and J.E. Minton. 2003. Early expression of toll-like receptors in porcine jejunal epithelial cells following treatment with lipopolysaccharide, Salmonella Typhimurium, and Salmonella Choleraesuis. 84th Conference of Research Workers in Animal Diseases, p. Abstr.. Chicago, IL.

Elsasser, T.H, S. Kahl, J.L. Sartin, A. Martinez, F. Cuttitta, R. Fayer, and J. Hinson. 2003. Muscle protein tyrosine nitration patterns during chronic subclinical intramuscular parasitism: Co-localization to fiber type and ubiquitin. J. Anim. Sci. 81(Suppl. 2):

Skjolaas, K.A., T. E. Burkey J.E. Minton. 2003. Time course of expression of interleukin 8 (IL-8), macrophage migration inhibitory factor (MIF) and osteopontin (OST) mRNA in swine jejunal epithelial cells following apical lipopolysaccharide (LPS) and Salmonella enterica serovar Typhimurium and Choleraesuis. 84th Conference of Research Workers in Animal Diseases, p. Abstr.. Chicago, IL.

Balaji, R., J. E. Minton, C. R. Ross, and F. Blecha. 2003. TREM-1, a member of the immunoglobulin superfamily: Cloning and expression studies. 90th Annual Meeting of the American Association of Immunologists, Abstr. 79.15. Denver, CO.

Burkey, T.E., S.S. Dritz, J.C. Nietfeld, B.J. Johnson and J.E. Minton. 2003. Effect of dietary mannanoligosaccharide and sodium chlorate on bacterial shedding in weaned pigs challenged with Salmonella enterica serotype typhimurium (ST). J. Anim. Sci. 81(Suppl. 2):38.

Skjolaas, K. A., T. E. Burkey, M. R. Barker, S. S. Dritz, and J. E. Minton. 2003. Carbadox does not alter immune cell phenotypes in mesenteric lymph nodes of pigs challenged with Salmonella enterica serotype Typhimurium. J. Anim. Sci. 81(Suppl. 1):192.

Turner, J. L., S. S. Dritz, and J. E. Minton. 2003. Feeding seaweed extract to nursery pigs alters circulating thyroid hormones. J. Anim. Sci. 81(Suppl. 1):205.

Kentucky
Peer-reviewed:
Brown-Brandl, T., Y. Yanagi, X. Xin, R.S. Gates, R. Bucklin and G. Ross. 2003.
Telemetry system for measuring core body temperature in livestock and poultry. Applied Engineering in Agriculture. In press.

Chepete, H.J., H. Xin, M. Puma, and R.S. Gates. 2003. Heat and moisture production of pullets and layers with or without the contribution of feces. ASHRAE Transactions. Accepted for publication.

Yanagi, T., H. Xin and R.S. Gates. 2002. A research facility for studying poultry responses to heat stress and its relief. Applied Engineering in Agriculture 18(2): 255-260.

Yanagi, T., H. Xin and R.S. Gates. 2002. Optimization of partial surface wetting to cool caged laying hens. Transactions of the ASAE 45(4): 1091-1100.

Xin, H., R.S. Gates, M.C. Puma and D.U. Ahn. 2002. Drinking water temperature effects on laying hens subjected to warm cyclic environments. Poultry Science 81:608-617.

Conferences and Proceedings

Bicudo, J.R. and Gates, R.S. (2002). Water consumption, air and water temperature issues related to portable water systems for grazing cattle. Presented at 2002 ASAE Annual International Meeting / CIGR XV World Congress, July 28-31, Chicago, IL, Paper No. 024052, ASAE, 2950 Niles Road, St. Joseph, MI.

Bicudo, J.R., Agouridis, C.T., Workman, S.R., Gates, R.S., and Vanzant, E.S. (2003). Effects of air and water temperature, and stream access on grazing cattle water intake rates. Presented at 2003 ASAE Annual International Meeting, July 27-30, Las Vegas,NV. Paper No. 034034, ASAE, 2950 Niles Road, St. Joseph, MI

Davis, J.D., Vanzant, E.S., Purswell, J.L., Green, A.R., Bicudo, J.R., Gates, R.S., Holloway, L., and Smith, W. (2003). Methods of remote, continuous temperature detection in beef cattle. Presented at 2003 ASAE Annual International Meeting, July 27-30, Las Vegas, NV, Paper No. 0340008, ASAE, 2950 Niles Road, St. Joseph, MI.

Green, A.R., J.L. Purswell, J.D. Davis, R.S. Gates, L. Lawrence R. Coleman and E.F.Wheeler. 2003. Methods for assessing horse well being during transport. Presented at 2003 ASAE Annual International Meeting, July 27-30, Las Vegas, NV, Paper No. 034092, ASAE, 2950 Niles Road, St. Joseph, MI.

Persyn, K.E., H. Xin, A. Ikeguchi and R.S. Gates. 2003. Feeding behaviors and pecking force of chicks with or without beak trimming. 2003. Presented at 2003 ASAE Annual International Meeting, July 27-30, Las Vegas, NV, Paper No. 034005, ASAE, 2950 Niles Road, St. Joseph, MI.

Purswell, J.L., A.R. Green, J.D. Davis, R.S. Gates, L. Lawrence R. Coleman. 2003. Measuring ventilation of a horse trailer during transport. Presented at 2003 ASAE Annual International Meeting, July 27-30, Las Vegas, NV, Paper No. 034091, ASAE, 2950 Niles Road, St. Joseph, MI.

Missouri
USDA
Peer-reviewed:
Gaines, A.M., J.A. Carroll, and G.L. Allee. 2003. Nursery performance of pigs injected with exogenous glucocorticoids at weaning. Journal of Animal and Veterinary Advances 2(1):22-26.

Willard, S.T., J.A. Carroll, M.A. Lammoglia, C.N. Kemper-Green, T.H. Welsh, Jr., and R.D. Randel. 2003. Plasma concentrations of growth hormone, growth hormone secretory dynamics and changes in follicular development during the bovine estrous cycle. Journal of Animal and Veterinary Advances 2(2):102-109.

Sterle, J.A., T.C. Cantley, R.L. Matteri, J.A. Carroll, M.C. Lucy and W.R. Lamberson. 2003. Effect of recombinant porcine somatotropin on fetal and placental growth in gilts with reduced uterine capacity. Journal of Animal Science 81:765-771.

Seaman-Bridges, J.S., J.A. Carroll, T.J. Safranski and E.P. Berg. 2003. Short- and long-term influence of perinatal dexamethasone treatment on swine growth. Domestic Animal Endocrinology 24(3):193-208.

Carroll, J.A., A.M. Gaines, J.D. Spencer, G.L. Allee, H.G. Kattesh, and M.E. Zannelli. 2003. Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs: I. Effects on the immune axis when fed diets containing spray-dried plasma. Domestic Animal Endocrinology 24:341-351.


Gaines, A.M., J.A. Carroll, G.F. Yi, G.L. Allee, and M.E. Zannelli. 2003. Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs: II. Effects on the immune axis when fed simple or complex diets containing no spray-dried plasma. Domestic Animal Endocrinology 24:353-365.

Frank, J.W., J.A. Carroll, G.L. Allee, and M.E. Zannelli. 2003. The effect of thermal environment and spray-dried plasma on the acute-phase response of pigs challenged with lipopolysaccharide. Journal of Animal Science 81:1166-1176.

Liu, Y.L., D.F. Li, L.M. Gong, G.F. Yi, A.M. Gaines, and J.A. Carroll. Effects of fish oil supplementation on performance as well as immunological, adrenal and somatotrophic responses of weaned pigs after Escherichia coli lipopolysaccharide challenge. Journal of Animal Science (Accepted 3/11/03)

Turk, J.R., J.A. Carroll, M.H. Laughlin, T.R. Thomas, M. Sturek, D.K. Bowles, and J. Casati. C-reactive protein correlates with macrophage accumulation in coronary arteries of pigs fed a high fat and cholesterol diet. Journal of Applied Physiology (Accepted 5/7/03).

Carroll, J.A., T.J. Fangman, A.K. Hambach and C.E. Wiedmeyer. The acute phase response in pigs experimentally infected with Escherichia coli (E. coli) and treated with systemic bactericidal antibiotics. Livestock Production Science (In Press)

Salfen, B.E., J.A. Carroll, and D.H. Keisler. Endocrine responses to short-term feed deprivation in weanling pigs. Journal of Endocrinology (In Press)

Abstracts:

Frank, J.W., J.A. Carroll, G.L. Allee, and M.E. Zannelli. 2003. Impact of thermal environment and spray-dried plasma on the acute phase response of pigs challenged with lipopolysaccharide (LPS). Journal of Animal Science 81 (Supp. 2):63 (Abstract #255).

Gaines, A.M., J.A. Carroll, G.L. Allee, J. Connor, and D.C. Kendall. 2003. Evaluation of dexamethasone injection on preweaning growth performance of neonatal pigs under commercial conditions. Journal of Animal Science 81 (Supp. 2):30 (Abstract #119).

Strauch, T.A., J.A. Carroll, T.J. Fangman, C.E. Wiedmeyer, and A.K. Hambach. 2003. Immune response in Escherichia coli challenged pigs exhibiting a febrile response in absence of elevated TNF-alpha. Journal of Animal Science 81 (Supp. 2):64 (Abstract #256).

Salfen, B.E. and J.A. Carroll. 2003. Endocrine responses to short-term feed deprivation in weanling pigs. Journal of Animal Science 81 (Supp. 2):63 (Abstract #252).

Yi, G.F., J.A. Carroll, G.L. Allee, A.M. Gaines, D.C. Kendall, Y. Toride, and I. Izuru. 2003. Impact of glutamine and spray-dried plasma on growth performance, small intestinal morphology, and immune responses in Escherichia coli K88+ challenged pigs. Journal of Animal Science 81 (Supp. 2):38 (Abstract #150).

Strauch, T.A., J.A. Carroll, E.L. Berg, and B.E. Salfen. 2003. Effects of Ralgro implantation to gestating sows on sow and piglet performance and components of the somatotrophic axis. Journal of Animal Science 81 (Supp. 1):94 (Abstract #374).

Salfen, B.E., J.A. Carroll, and D.H. Keisler. 2003. Endocrine responses to 72h feed deprivation in weanling pigs. Journal of Animal Science 81 (Supp. 1):101 (Abstract #402).

Salfen, B.E., J.A. Carroll, and D.H. Keisler. 2003. Endocrine responses to short-term feed deprivation in weanling pigs. University of Missouri?s Life Sciences Week.

Turk, J.R., J.A. Carroll, M.H. Laughlin, T.R. Thomas, and D.K. Bowles. 2003. C-reactive protein correlates with coronary artery disease in pigs fed high fat and cholesterol diets. University of Missouri?s Life Sciences Week.

University of Missouri-Columbia
Peer-reviewed:
Roberts, C.A., Spiers, D.E., Karr, A.L., Benedict, H.R., Sleper, D.A., Eichen, P.A., West, C.P., Piper, E.L. and Rottinghaus, G.E. 2002. Use of a Rat Model to Evaluate Tall Fescue Seed Infected with Introduced Strains of Neotyphodium coenophialum. J. Agric. Food Chem. 50: 5742.

Al-Tamimi, H., Rottinghaus, G.E., Spiers, D.E., Spain, J., Chatman, D., Eichen, P.A., Carson, T.L. and Hill, N.S. 2003. Thermoregulatory response of dairy cows fed ergotized barley during summer heat stress. J. Vet. Diag. Invest. 15: 355.

Mader, T.L., Holt, S.M., Hahn, G.L., Davis, M.S. and Spiers, D.E. 2002. Feeding strategies for managing heat load in feedlot cattle. J. Anim. Sci. 80: 2373

Gadberry, M.S., Denard, T.M., Spiers, D.E. and Piper,E.L. 2003. Effects of feeding ergovaline on lamb performance in a heat stress environment. J. Anim. Sci. 81: 1538.

Abstracts:
Spurlin, K.M.., D. E. Spiers, M. Ellersieck, and J.N. Spain. 2002. Effects of simultaneous evaluation of cooling strategies on production responses and intake behavior during heat challenge in dairy cattle. J. Dairy Sci.85(Suppl. 1):206.

Oetting, E., J. Spain, and J. Sampson. 2002. The effects of strategic cooling on thermal balance of late gestation dairy cows. J. Dairy Sci. (Suppl. 1):309.

Spurlin, K.M.., J. Porter, M. Ellersieck, and J.N. Spain. 2002. Effects of mild heat challenge on rate of passage and rumen fermentation in lactating dairy cattle. J. Dairy Sci. (Suppl. 1):183.

Spain, J.N., M. Lucy, and D. Spiers. 2002. The effects of environmental stress on the performance of dairy cattle. J. Dairy Sci. (Suppl. 1):56.

Kolath, S.J., P.M. Coussens, S. Sipkovsky, S.J. Wilson, D.E. Spiers, J.N. Spain, and M.C. Lucy. 2003. Microarray analysis of gene expression in dominant ovarian follicles (DF) from heat stress (HS) and thermoneutral (TN) heifers. 36th Annual Meeting Midwestern Section American Society of Animal Science. Des Moines, Iowa.

McVicker, L.E., D.E. Spiers, J.E. Williams, K.J. Barnhart, L.N. Thompson, A. Al_Haidary, and D.P. Colling. 2003. Use of dietary seaweed treatment to reduce heat strain in cattle. 36th Annual Meeting Midwestern Section American Society of Animal Science. Des Moines, Iowa.

Thompson, L.N., J.E. Williams, K.J. Barnhart, L.E. McVicker, D.E. Spiers, and D.P. Colling. 36th Annual Meeting Midwestern Section American Society of Animal Science. Des Moines, Iowa.

Williams, J.E., L. Thompson, K.J. Barnhart, D.E. Spiers, and D.P. Colling. 2002. Use of Ascophyllum nodosum for alleviation of heat stress in cattle. 16th International Congress on Biometeorology, October 28 - November 1; Kansas City, Missouri.

Al-Haidary, A., M.J. Leonard, and D.E. Spiers. 2002, The effect of progressively higher levels of heat challenge on temperature control of cattle. Proceedings of the 16th International Congress on Biometeorology, October 28 - November 1; Kansas City, Missouri, pg. 94-97.

McVicker, L.E., M.J. Leonard, and D.E. Spiers. 2002. Evaluation of feedlot cattle response to summer heat in open or shaded pens. Proceedings of the 16th International Congress on Biometeorology, October 28 - November 1; Kansas City, Missouri, pg. 104-108.

Al-Tamimi, H.A., D.E. Spiers, J. Lakritz, M. Ellersieck, and G. Rottinghaus. 2002. Effects of exogenous nitric oxide on thermoregulatory characteristics of cattle experiencing fescue toxicosis and cyclical heat stress. Proceedings of the 16th International Congress on Biometeorology, October 28 - November 1; Kansas City, Missouri, pg. 109-115.

Spain, J., D.E. Spiers, J. Sampson, and K. Spurlin. 2002. Effect of ambient conditions on thermal balance of dairy cattle. 16th International Congress on Biometeorology, October 28 - November 1; Kansas City, Missouri.

Parkhurst, A.M., D.E. Spiers, T.L. Mader, and G.L. Hahn. 2002. What is the definition of heat stress threshold? Proceedings of the 16th International Congress on Biometeorology, October 28 - November 1; Kansas City, Missouri, pg. 162-165.

Eichen, P.A., M.J. Leonard, M.A. Kozma, B.M. Kronk, L.E. McVicker, D.E. Spiers, and D.P. Colling. 2003. Is iodide responsible for the heat-relief effects of Ascophyllum nodosum? J. Anim. Sci. 81(Suppl. 1): 155.

Spiers, D.E., L.E. McVicker, J.E. Williams, P.A. Eichen, L. Thompson, G. Rottinghaus, and D.P. Colling. 2003. Monitoring fescue toxicosis in a pasture environment and evaluating the effect of prior treatment with Ascophyllum nodosum . J. Anim. Sci. 81(Suppl. 1): 156.

Invited Presentations:
Heat Stress and the Associated Effects on Thermal Balance of the Dairy Cow. Concordia Annual Dairy Day, MO, November 2002.

Spiers, D.E., Nuevas alternativas para medir y reducir el estres calorico en ranado bovino. Reunion Internacional Sobre Produccion de Carne y Leche en Climas Calidos. Mexicali, Baja California, October 3-4, pg. 57-64.

Nebraska
USDA
Brown-Brandl, T.M., D.D. Jones, and W.E. Woldt. 2003. Evaluating modeling techniques for livestock heat stress prediction. ASAE Paper #034009. St. Joseph, MI.

Brown-Brandl, T.M., J.A. Nienaber, G.L. Hahn, R.A. Eigenberg, and A.M. Parkhurst. 2003. Dynamic responses of feeder cattle to simulated heat waves. Proc. Int?l. Symp on Energy and Protein Metabolism. Sept 13-18, 2003. Rostock-Warnemunde, Germany.

Brown-Brandl, T.M., J.A. Nienaber, H. Xin, and R.S. Gates. 2003. A literature review of swine heat and moisture production. Proc. Swine Housing Conference, Oct 11-14, 2003. Raleigh, NC.

Brown-Brandl, T.M., J.A. Nienaber, R.A. Eigenberg, G.L. Hahn, and H. Freetly. 2003. Thermoregulatory responses of feeder cattle. J. Thermal Biology 28:149-157.

Brown-Brandl, T.M., J.A. Nienaber, R.A. Eigenberg, T.L. Mader, J.L. Morrow, and J.W. Dailey. 2003. Relative heat tolerance among cattle of different genetics. ASAE Paper 034035. St. Joseph, MI.

Brown-Brandl, T.M., T. Yanagi, H. Xin, R.S. Gates, R. Bucklin, and R.S. Gates. 2003. A new telemetry system for measuring core body temperature in livestock and poultry. Applied Engineering in Agriculture (Accepted 4/10/03)

Eigenberg, R.A., J.A. Nienaber, and T.M. Brown-Brandl. 2003.Development of a livestock safety monitor for cattle. ASAE Paper 032338. St. Joseph, MI.

Eigenberg, R.A., T. Brown-Brandl, and J.A. Nienaber. 2002. Development of a respiration rate monitor for swine. Transactions of the ASAE 45(5):1599-1603.

Freetly, H.C., J.A. Nienaber, and T.M. Brown-Brandl. 2003. Relationship between aging and nutritionally controlled growth rate on heat production of heifers. J. Anim. Sci. 81:1847-1852.

Freetly, H.C., J.A. Nienaber, and T.M. Brown-Brandl. 2003. Heat production of growing heifers that differ in proportions of Bos indicus and Bos Taurus genetics. Proc. Int.l Symp on Energy and Protein Metabolism. Sept 13-18, 2003 Rostock-Warnemunde, Germany.

Hahn, G.L., T.L. Mader, J.A. Harrington, J.A. Nienaber, and K.L. Frank. 2002. Living with climatic variability and potential global change: climatological analyses of impacts on livestock performance. Proc., 16th Int?l Congress on Biometeorology, 45-49. Kansas City, MO.

Kerr, B.J., J.T. Yen, J.A. Nienaber, and R.A. Easter. 2003. Influences of dietary protein level, amino acid supplementation and environmental temperature on performance, body composition, organ weights and total heat production of growing pigs. J. Anim. Sci. 81:1998-2007.

Nienaber, J.A. and G.L. Hahn. 2002. Effects of high environmental temperatures on growing-finishing swine. Proc., 16th Int?l. Congress on Biometeorology, 116-121. Kansas City, MO

Nienaber, J.A., G.L. Hahn, T.M. Brown-Brandl and R.A. Eigenberg. 2003. Heat stress climatic conditions and the physiological responses of cattle. Fifth Int?l Dairy Housing Conference, 255-262. Ft. Worth, TX

University of Nebraska-Lincoln
Peer-reviewed:
Davis, M.S., T. L. Mader, S. M. Holt, and A.M. Parkhurst, 2003. ?Strategies to reduce feedlot cattle heat stress effects on tympanic temperature?, Journal of Animal Science. (in review),

Yen, J.T., B.J.Kerr, R.A.Easter, A.M. Parkhurst 2003. ?Difference in rates of portal absorption between crystalline and protein-bound lysine and threonine in growing pigs fed once daily? Journal of Animal Science.( in review)

Mader, T. L., S. M. Holt, G. L. Hahn, M. S. Davis and D. E. Spiers. 2002. Feeding strategies for managing heat load in feedlot cattle. J. Anim. Sci. (in press).

Peer-Reviewed Proceedings

Brown-Brandl, T.M., J.A. Nienaber, G.L. Hahn, R.A. Eigenberg, and A.M. Parkhurst. 2003. Dynamic responses of feeder cattle to simulated heat waves. Proc. Int?l. Symp on Energy and Protein Metabolism. Sept 13-18, 2003. Rostock-Warnemunde, Germany.

Brown-Brandl, T.M., J.A. Nienaber, R.A. Eigenberg, T.L. Mader, J.L. Morrow, and J.W. Dailey. 2003. Relative heat tolerance among cattle of different genetics. ASAE Paper 034035. St. Joseph, MI.

Parkhurst, A. M., D.A.Spiers, T.L.Mader, and G. L. Hahn,2002 " Spline models fro estimating heat stress thresholds in cattle", Proc. 14th Annual Kansas State University Conference on Applied Statistics in Agriculture. 137_148 Kansas State Univ., Manhattan, KS

Lan, L., A. M. Parkhurst, D. A. Spiers, K. M. Eskridge, and G. L. Hahn. 2002. "Using Nonlinear Fixed and Mixed Models to Study Acclimation to Heat Stress in Cattle" Proc. 14th Annual Kansas State University Conference on Applied Statistics in Agriculture. 149_163 Kansas State Univ., Manhattan, KS

Parkhurst, A. M., D. A. Spiers, T. L. Mader, and G. L. Hahn, 2002. " What is the Definition of Heat Stress Threshold?? Proceedings of 15th Conference on Biometeorology and Aerobiology 162-165 co-sponsored by American Meteorological Society and International Society of Biometry, Kansas City KS

New York

Peer-reviewed:
Hillman, Peter, CN Lee and S. Willard. 2003. Thermal responses of dairy cows to heat stress with and without freestall cooling. Presented at the July 28-July 30, 2003, ASAE Annual International Meeting at Las Vegas, NV. Paper No. 034036, ASAE, 2950 Niles Rd., St. Joseph, MI 49085-9659, USA

Hillman, Peter, S. Willard, CN Lee and SD Kennedy. 2003. Efficacy of a vaginal temperature logger to record body temperatures of dairy cows. Presented at the July 28-July 30, 2003, ASAE Annual International Meeting at Las Vegas, NV. Paper No. 034011, ASAE, 2950 Niles Rd., St. Joseph, MI 49085-9659, USA


Tennesee

Peer-reviewed:
Heo, J., H. G. Kattesh, R. L. Matteri, J. D. Grizzle, and J. D. Godkin. 2003. Partial nucleotide sequence of the porcine corticosteroid-binding globulin (CBG) cDNA and specification of CBG expression sites in postnatal pigs. Dom. Anim. Endo. 24(3):257-64.

Roberts, M. P., H. G. Kattesh, G. A. Baumbach, B. B. Gillespie, J. D. Godkin, J. F. Schneider, and A. M. Saxton. 2003. Age-related changes in porcine corticosteroid-binding globulin (pCBG) as determined by an enzyme-linked immunosorbent assay. Dom. Anim. Endo. 24(4):323-39.

Carroll, J. A., A. M. Gaines, J. D. Spencer, G. L. Allee, H. G. Kattesh, M. P. Roberts, and M. E. Zannelli. 2003. Effect of menhaden fish oil supplementation and lipopolysaccharide exposure on nursery pigs: I. Effects on the immune axis when fed diets containing spray-dried plasma. Dom. Anim. Endo. 24(4):341-51.

Heo, J., H. G. Kattesh, J. J. E. Doré, M. P. Roberts, and J. D. Godkin. 2003. In vitro liver synthesis and plasma levels of corticosteroid-binding globulin (CBG) in the piglet. J. Anim. Vet. Adv. 2(2):82-4.

Heo, J., H. G. Kattesh, M. P. Roberts, and J. F. Schneider. 2003. Plasma levels of cortisol and corticosteroid-binding globulin (CBG) and hepatic CBG mRNA in pre- and postnatal pigs. Dom. Anim. Endo. Accepted.
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