SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: W171 : Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock
- Period Covered: 01/01/2003 to 12/01/2003
- Date of Report: 03/05/2004
- Annual Meeting Dates: 01/09/2004 to 01/10/2004
Participants
Hu, Ching Yuan (chingyuan.hu@oregonstate.edu) - Oregon State University; Mirando, Mark (mmirando@csrees.usda.gov) CSREES; Rorie, Rick (rrorie@uark.edu) - University of Arkansas; Rosenkrans, Charles (crosenkr@uark.edu) - University of Arkansas; Seidel, George (gseidel@colostate.edu) - Colorado State University; Godke, Robert (rgodke@agctr.lsu.edu) - Louisiana State University; Leibo, Stanley (sleibo@acres.org) - Audubon Center for Research of Endangered Species; Wheeler, Matt (mbwheele@uiuc.edu) - University of Illinois; Youngs, Curt (cryoungs@iastate.edu) - Iowa State University; Menino, Fred (alfred.r.menino@oregonstate.edu) - Oregon State University; Mapletoft, Reuben (reuben.mapletoft@usask.ca) - University of Saskatchewan; White, Kenneth (kwhite@cc.usu.edu) - Utah State University; Keefer, Carol (ckeefer@umd.edu) - University of Maryland; Krisher, Rebecca (rkrisher@purdue.edu) - Purdue University; Schrick, Neal (fschrick@utk.edu) - University of Tennessee; Edwards, Lannett (jedwards@utk.edu) - University of Tennessee;
Minutes from the W-171 Meeting
Portland, Oregon
January 10, 2004
The meeting was opened at 6:05 pm by Bob Godke, the W-171 secretary, since Rick Rorie, the chairperson, was held up by weather conditions.
New Business: Dr. Mark Miranda the CSREES Administrator made opening comments and review the status of the USDA budget.
W-171 Administrative Advisor, Dr. C. Y. Hu from Oregon State University, then gave opening comments relating to the procedure to submit the revised project proposal. Dr. Hu indicated that the project review will occur in May of 2004, if the group can get the revised project into the NIMSS system as soon as possible. Dr. Hu then reviewed the steps that needed to be completed to get the revised W-171 proposal sent out for outside review. He indicated that once the revised proposal was submitted to the NIMSS system, each station was to submit Appendix E from their experiment station activating that station scientists to become members of the new Mullti-State Research Project.
New members, Rebecca Krisher from Perdue, Neal Schrick from the University of Tennessee and Lannett Edwards from the University of Tennessee were welcomed to the group.
Old Business: Matt Wheeler reported on the project rewrite and thanked everyone who worked with him to get the revised proposal completed by this meeting. Bob Godke report that he had finished the Critical Review section and thanked the members from each station for contributing the information requested over the last two and a half months. He indicated that there were over 70 pages of publication alone reported by members of the contributing stations. Curt Youngs indicated that the revised project proposal prepared by Matt Wheeler and the critical review prepared by Bob Godke should be e-mailed to him, so that he could get into the NIMSS system the following week.
Dr. Hu indicated that we needed to suggest names of respected scientists in the field as potential reviewers of the revised project. A list of 10 reproductive physiologists was suggested by the members present. Bob Godke was to contact members from the list to identify four scientists that would agree to review the proposal. Godke was asked to contact four or five scientists from the list to conduct the outside review and then send their names and addresses to the administrative advisor.
Station project reports were made in an abbreviated format. It was suggested that we contact IFIS to obtain funds to support a seminar program. Baton Rouge, Louisiana (LSU) was selected for next Technical Committee meeting of new W-1171 in February of 2005. Bob Godke was elected as the new W-171 Chairperson and Rebecca Krisher was elected as the new Secretary by a unanimous vote.
A motion was made and seconded to adjourn the meeting at 10:10 pm.
Respectfully submitted,
Robert A. Godke
W-171 Secretary
Portland, Oregon
January 10, 2004
The meeting was opened at 6:05 pm by Bob Godke, the W-171 secretary, since Rick Rorie, the chairperson, was held up by weather conditions.
New Business: Dr. Mark Miranda the CSREES Administrator made opening comments and review the status of the USDA budget.
W-171 Administrative Advisor, Dr. C. Y. Hu from Oregon State University, then gave opening comments relating to the procedure to submit the revised project proposal. Dr. Hu indicated that the project review will occur in May of 2004, if the group can get the revised project into the NIMSS system as soon as possible. Dr. Hu then reviewed the steps that needed to be completed to get the revised W-171 proposal sent out for outside review. He indicated that once the revised proposal was submitted to the NIMSS system, each station was to submit Appendix E from their experiment station activating that station scientists to become members of the new Mullti-State Research Project.
New members, Rebecca Krisher from Perdue, Neal Schrick from the University of Tennessee and Lannett Edwards from the University of Tennessee were welcomed to the group.
Old Business: Matt Wheeler reported on the project rewrite and thanked everyone who worked with him to get the revised proposal completed by this meeting. Bob Godke report that he had finished the Critical Review section and thanked the members from each station for contributing the information requested over the last two and a half months. He indicated that there were over 70 pages of publication alone reported by members of the contributing stations. Curt Youngs indicated that the revised project proposal prepared by Matt Wheeler and the critical review prepared by Bob Godke should be e-mailed to him, so that he could get into the NIMSS system the following week.
Dr. Hu indicated that we needed to suggest names of respected scientists in the field as potential reviewers of the revised project. A list of 10 reproductive physiologists was suggested by the members present. Bob Godke was to contact members from the list to identify four scientists that would agree to review the proposal. Godke was asked to contact four or five scientists from the list to conduct the outside review and then send their names and addresses to the administrative advisor.
Station project reports were made in an abbreviated format. It was suggested that we contact IFIS to obtain funds to support a seminar program. Baton Rouge, Louisiana (LSU) was selected for next Technical Committee meeting of new W-1171 in February of 2005. Bob Godke was elected as the new W-171 Chairperson and Rebecca Krisher was elected as the new Secretary by a unanimous vote.
A motion was made and seconded to adjourn the meeting at 10:10 pm.
Respectfully submitted,
Robert A. Godke
W-171 Secretary
Accomplishments
It was demonstrated that phosphatase 1 is an important regulator of expression of tPA, PAI-1 and b-actin in bovine oocyte-cumulus cell complexes matured in vitro.
Oocyte plasma membrane proteins were investigated that are potentially involved in sperm-oocyte interaction.
Two potential boimarkers were identified for evaluation of oocyte maturation.
An alternative culture system for in vitro embryo manipulation in livestock was fabricated and tested.
Improvements were made in systems of in vitro production of bovine embryos are resulting in embryos fairly similar to those produced in vivo.
The generation of artificial gametes from somatic cells was studied and improvement was made in the rate of meiosis-like divisions.
The levels of epigenetic markers of donor cells at different passages, tissue type and cell cycle stage for nuclear transfer was investigated.
It was shown that milk production and piglet growth can be significantly increased by the introduction of single genes, which are involved in milk synthesis and intestinal maturation, respectively.
Nuclear transfer studies, using colcemid treated oocytes as recipient cytoplasts resulted in an increase in the number of blastocysts and hatched blastocysts.
The degree of differentiation effects reprogramming capacity within a single lineage of donor cells used in somatic cell NT.
Appropriate expression of DNA methyltransferases was found to be important for normal mammalian development to proceed in both cloned embryos and others produced by natural mating or in vitro.
To demonstrate how germplasm from deceased bulls can be saved, epididymal sperm stored in the testes for 24 h at 4ºC was used in a standard in vitro fertilization procedure, producing good quality embryos and subsequent pregnancies in recipient beef females.
A very sensitive test for fertility in cattle was developed, using competitive fertilization procedures.
Bovine somatotropin was shown to adversely affect fertility, when given the second week of gestation.
A simple, inexpensive procedure was developed for cryopreservation of bovine embryos.
Calves resulting from sexed sperm were shown to be normal except being about 90% of the selected sex, rather than 50:50.
Investigated the potential usefulness of the intralabial route of administration of prostaglandin F2alpha for synchronization of postpartum dairy cows for use as recipients.
Oocyte plasma membrane proteins were investigated that are potentially involved in sperm-oocyte interaction.
Two potential boimarkers were identified for evaluation of oocyte maturation.
An alternative culture system for in vitro embryo manipulation in livestock was fabricated and tested.
Improvements were made in systems of in vitro production of bovine embryos are resulting in embryos fairly similar to those produced in vivo.
The generation of artificial gametes from somatic cells was studied and improvement was made in the rate of meiosis-like divisions.
The levels of epigenetic markers of donor cells at different passages, tissue type and cell cycle stage for nuclear transfer was investigated.
It was shown that milk production and piglet growth can be significantly increased by the introduction of single genes, which are involved in milk synthesis and intestinal maturation, respectively.
Nuclear transfer studies, using colcemid treated oocytes as recipient cytoplasts resulted in an increase in the number of blastocysts and hatched blastocysts.
The degree of differentiation effects reprogramming capacity within a single lineage of donor cells used in somatic cell NT.
Appropriate expression of DNA methyltransferases was found to be important for normal mammalian development to proceed in both cloned embryos and others produced by natural mating or in vitro.
To demonstrate how germplasm from deceased bulls can be saved, epididymal sperm stored in the testes for 24 h at 4ºC was used in a standard in vitro fertilization procedure, producing good quality embryos and subsequent pregnancies in recipient beef females.
A very sensitive test for fertility in cattle was developed, using competitive fertilization procedures.
Bovine somatotropin was shown to adversely affect fertility, when given the second week of gestation.
A simple, inexpensive procedure was developed for cryopreservation of bovine embryos.
Calves resulting from sexed sperm were shown to be normal except being about 90% of the selected sex, rather than 50:50.
Investigated the potential usefulness of the intralabial route of administration of prostaglandin F2alpha for synchronization of postpartum dairy cows for use as recipients.
Impacts
- Information from this multistate project will improve techniques employed for cloning and genetic engineering in livestock, impacting on animal agriculture, animal and human health.
- Development of efficient oocyte and embryo culture systems allow for improvement of livestock via associated technologies.
- Procedures developed for vitrifying bovine embryos will decrease costs and increase flexibility of cryopreserving embryos.
- Demonstration that calves from sexed semen are normal allow farmers can use these procedures with minimal concerns.
- Beta-lactalbumin and IGF transgenic swine may allow for improvement of piglet growth and health in swine production systems.
- Impact 6; Understanding of the role of bST in reproduction may lead to development of procedures to avoid adverse effects on fertility; Impact 7; The competitive fertilization model developed enables accurate comparisons of treatments for fertility at lower cost;Impact 8; Cloning studies identified cell types best-suited for use in cloning and oocyte treatment to increase the number of NT morulae and blastocysts for transfer.
Publications
Refereed Journal Articles/Refereed Book Chapters
ARKANSAS
Baublits, R.T., A.H. Brown, Jr., Z.B. Johnson, C.F. Rosenkerans, Jr., R.W. Rorie and B.A. Sandelin. 2003. Variation in the percentage of female births over time in five breeds of registered cattle. Prof. Anim. Sci. 19:204-210.
Steelman, C.D., R.W. McNew, R.B. Simpson, R.W. Rorie, J.M. Phillips and C.F. Rosenkrans, Jr. 2003. Evaluation fo alternative tactics for management of insecticide-resistant horn flies (diptera muscidae). J. Econ. Entomol. 96:892-901.
CALIFORNIA
Bertolini, M., J.B. Mason, S.E. Beam, G.F. Carneiro, M.L. Sween, D.J. Kominek, A.L. Moyer, T.R. Famula, R.D. Sainz and G.B. Anderson. 2002. Morphology and morphometry of in vitro-and in vitro-produced concepti from early pregnancy to term and association with high birth weights. Theriogenology 58:973-994.
Bertolini, M., S.E. Beam, H. Shim, L.R. Bertolini, A.L. Moyer, T.R. Famula and G.B. Anderson. 2002. Growth, development and gene expression by in vitro-and in vitro-produced day-7 and day-16 bovine embryos. Molecular Reproduction and Development 63:318-328.
Carmona, E., W. Weerachatyanukul, T. Soboloff, A. L. Fluharty, D. White, L. Promdee, M. Ekker, T. Berger, M. Buhr, and N. Tanphaichitr. 2002. Arylsulfatase A is present on the pig sperm surface and is involved in sperm-zona pellucida binding. Dev. Biol. 247:182.
Sartini, B.L. and T. Berger. 2003. Changes in oocyte plasma membrane binding sites on boar spermatozoa with capacitation and acrosome reactions. Reproduction 125:865.
Rui, R., H. Shim, A.L. Moyer, D. Anderson, M.C. Penedo, J.D. Rowe, R.H. BonDurant and G.B. Anderson. Attempts to enhance production of chimeras from porcine embryonic germ cells and preimplantation porcine embryos. Theriogenology (In press).
Maga, E.A., G. Sargent, H. Zeng, S. Pati, D. Zarling, S. M. Oppenheim, N.M.B. Collette, A.L. Moyer, J.S. Conrad-Brink, J. D. Rowe, R.H. BonDurant, G.B. Anderson, and J.D. Murray. Increased efficiency of transgenic livestock production. Transgenic Reseach (In press).
CANADA
Bo, G.A. and Mapletoft, R.J., 2003. Superovulacion en Bovinos. En: Reproduccion en los Animales Domisticos. Tomo II. R. Ungerfeld (Editor). Melibea Ediciones, pages 515-538.
Bo, G.A., Palasz, A.T. and Mapletoft, R.J., 2003. Criopreservacion de Embriones. En: Reproduccion en los Animales Domisticos. Tomo II. R. Ungerfeld (Editor). Melibea Ediciones, pages 551-566.
Vassena, Rita, Mapletoft, Reuben J., Allodi, Stefano, Singh, Jaswant, Adams, Gregg P., 2003. Morphology and Developmental Competence of Bovine Oocytes relative to Follicular Status. Theriogenology, 60, 923-932.
Colazo, Marcos German, Kastelic, John Patrick, Mapletoft, Reuben John., 2003. Effects of Estradiol Cypionate (ECP) on Ovarian Follicular Dynamics, Synchrony of Ovulation and Fertility in CIDR-based, Fixed-time AI Programs in Beef Heifers. Theriogenology, 60, 855-865.
Martinez, M.F., Mapletoft, R.J., Kastelic, J.P. and Carruthers, T.D., 2003. The Effects of Three Gonadorelin Products on Luteinizing Hormone Release, Ovulation, and Follicular Wave Emergence in Cattle. Canadian Veterinary Journal, 44:125-131.
Vassena, R., Adams, G.P., Mapletoft, R.J., Pierson, R.A. and Singh, J, 2003. Ultrasound Image Characteristics of Ovarian Follicles in Relation to Oocyte Competence and Follicular Status in Cattle. Animal Reproduction Science, 76, 25-41.
COLORADO
Bass, L. D., D. J. Denniston, L. J. Maclellan, P. M. McCue, G. E. Seidel, Jr., and E. L. Squires. 2003. Methanol as a cryoprotectant for equine embryos. Theriogenology. Submitted.
Choi, Y. H., F. C. Landim-Alvarenga, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of capacitation of stallion sperm with polyvinylalcohol or bovine serum albumin on penetration of bovine zona-free or partially zona-removed equine oocytes. J. Anim. Sci. 81:2080-2087.
Coutinho da Silva, M. A., E. M. Carnevale, L. J. Maclellan, K. A. Preis, G. E. Seidel, Jr., and E. L. Squires. 2003. Oocyte transfer in mares with intrauterine or intraoviductal insemination using fresh, cooled and frozen stallion semen. Theriogenology. In Press.
Flint, A. F., P. L. Chapman, and G. E. Seidel, Jr. 2003. Heterospermic insemination of cattle using flow-sorted sperm. J. Anim. Sci. 81:1814-1822.
Franz, L. C., Y.-H. Choi, E. L. Squires, G. E. Seidel, Jr., and K. Hinrichs. 2003. Effects of roscovitine on maintenance of the germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection. Reproduction 125:693-700.
Garner, D. L., and G. E. Seidel, Jr. 2003. Past, present and future perspectives on sexing sperm. Can. J. Anim. Sci. 83:375-384.
Glover, M. D., and G. E. Seidel, Jr. 2003. Increased messenger RNA for allograft inflammatory factor-1, LERK-5, and a novel gene in 17.5-day relative to 15.5-day bovine embryos. Biol. Reprod. 69:1002-1012.
Li, G.-P., G. E. Seidel, Jr., and E. L. Squires. 2003. Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa. Theriogenology 59:1143-1155.
Li, G.-P., G. E. Seidel, Jr., and E. L. Squires. 2003. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology. In Press.
Lindsey, A. C., D. D. Varner, G. E. Seidel, Jr., J. E. Bruemmer, and E. L. Squires. 2003. Hysteroscopic or rectally guided, deep-uterine insemination of mares with spermatozoa stored at 18 h at either 5ºC or 15ºC prior to flow-cytometric sorting. Anim. Reprod. Sci. Submitted.
Mendes, J. O. B., Jr., P. D. Burns, J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Effect of heparin cleavage rates and embryo production with four bovine sperm preparation protocols. Theriogenology 60:331-340.
Seidel, G. E., Jr., 2003. Economics of selecting for sex: The most important genetic trait. Theriogenology 59:585-598.
Seidel, G. E., Jr. 2003. Sexing mammalian sperm ? Intertwining of commerce, technology, and biology. Anim Reprod Sci. In Press.
Zhang, M., K. H. Lu, and G. E. Seidel, Jr. 2003. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically-sorted, stained, and unsorted sperm from different bulls. Theriogenology 60:1657-1663.
CONNECTICUT
Enright, B. P., B. S. Jeong, X. Yang, and X. C. Tian. 2003a. Epigenetic characteristics of bovine donor cells for nuclear transfer: Levels of histone acetylation. Biol Reprod 69: 1525-1530.
Enright, B. P., C. Kubota, X. Yang, and X. C. Tian. 2003b. Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin a or 5-aza-2‘‘‘‘-deoxycytidine. Biol Reprod 69: 896-901.
Lee, J. W., X. C. Tian, and X. Yang. 2003a. Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection. Biol Reprod 68: 1341-1347.
Lee, J. W. et al. 2003b. Production of cloned pigs by whole-cell intracytoplasmic microinjection. Biol Reprod 69: 995-1001.
Savage, A. F. et al. 2003. Behavioral observations of adolescent holstein heifers cloned from adult somatic cells. Theriogenology 60: 1097-1110.
Suzuki, H., Y. Saito, N. Kagawa, and X. Yang. 2003. In vitro fertilization and polyspermy in the pig: Factors affecting fertilization rates and cytoskeletal reorganization of the oocyte. Microsc Res Tech 61: 327-334.
Tian, X. C., C. Kubota, B. Enright, and X. Yang. 2003. Cloning animals by somatic cell nuclear transfer - biological factors. Reprod Biol Endocrinol 1: 98.
Xu, J., and X. Yang. 2003. Will cloned animals suffer premature aging - the story at the end of clones‘‘‘‘ chromosomes. Reprod Biol Endocrinol 1: 105.
Yang, X. 2003. Toward therapeutic cloning and regenerative biology research forum: To clone or not to clone. Reprod Biol Endocrinol 1: 97.
ILLINOIS
Wheeler, M.B., Walters, E.M., Clark, S.G. (2003). Transgenic animals in biomedicine and agriculture:outlook for the future. Animal Reproduction Science 79:265-289.
Wheeler, M.B., Malusky, S.A. (2003). Transgenesis in mammals: status and perspectives. Acta Scientiae Veterinariae 31:119-136.
IOWA
Godke, R.A. and C.R. Youngs. 2003. Biotechnology: Embryo Transfer Technology. In (W. Pond, Ed.) Encyclopedia of Animal Science. Marcel Dekker, New York, NY (in press).
LOUISIANNA
Boediono, A., T. Suzuki and R.A. Godke. 2003. Comparison of hybrid and prurebred in vitro-derived cattle embryos during in vitro culture. Anim. Reprod. Sci. 78:1-11.
Cochran, R., M. Meintjes, D. Hylan, M. Sansinena, R. S. Denniston D. Paccamonti and R.A. Godke. 2003. Follicle growth in mares for oocyte production. In: From Epididymus to Embryo. Havemeyer Fdn. Monograph 6:51-54.
Devireddy,R.V., B. Fahrig, R.A. Godke and S.P. Leibo. The effect of extracellular ice and glycerol on boar sperm water transport parameters: Convergence of theoretical and empirical rates of optimal freezing. Molec. Reprod. Develop. (In press).
Godke, R.A., K. Hebert, A. James, R.S. Denniston and D. Paccamonti. 2003. The potential use of epididymal sperm from bulls and stallions for assisted fertilization. Havemeyer Fdn. Monograph (In press).
Godke, R.A. and C. R. Youngs. Biotechnology: Embryo technology in cattle. In: Encyclopedia of Animal Science. W.G. Pond & A.W. Bell (eds.). Marcel Dekker:New York (In press). (Book Chapter)
Gomez, M., C.E. Pope, A. Giraldo, L. Lyons, R. Harris, A. King, A. Cole, R. Godke and B. Dresser. 2003. Cloned African Wildcats born from a domestic cat. Cloning & Stem Cells. (Submitted).
Han, Y., Y. Han, R.S. Denniston, A. Boediono, C. Ziomek and Godke. Development of IVF-derived caprine embryos with four different somatic cell co-cultures. Small Ruminant Res. (Accepted).
Klump, A, A. Aljarrah, M., Sansinena M., D. Hylan, C. Pinto, B. Eilts, R. Godke and D. Paccamonti. 2003. Follicular aspiration during transition to advance the onset of cyclicity. Pferdeheilkunde Equine Vet. J. 19(6):609-610.
Landry, A.M., D.J. Landry, L.R. Gentry, H.L. Green, K.L. Koonce, Y. Echlard and R.A. Godke. Endocrine profiles and growth patterns of goats produced by nuclear transfer. Cloning & Stem Cells (In Final Prepartion).
Leibo, S.P. and N. Lane. The early history of gamete cryobiology. In: Life in the Frozen State. E. Benson, B. Fuller & N. Lane (eds.). Taylor & Francis:London. (In press). (Book Chapter)
Luster, S.M., M. Murakami, S.P. Leibo, R.S. Denniston Y. Echelard, and R.A. Godke. Pregnancies resulting from nuclear transfer embryos derived from vitrified bovine oocytes. Cryobiology (In press).
Massip A, Leibo SP, Blesbois E. Cryobiology of gametes and the breeding of domestic animals. In: Life in the Frozen State. E. Benson, B. Fuller & N. Lane (eds.) Taylor & Francis:London (In press). (Book Chapter)
Murakami, M., T.R. Davidson, C.E. Ferguson, Y. Echelard and R. A. Godke. Use of the amniotic cavity of the developing chick embryo for culturing bovine 8-cell embryos derived from somatic cell nuclear transfer. Molec. Reprod. Develop. (In press).
Murakami, M., S. Ideguchi, M. Fahrudin, T. Otoi, R.A. Godke and T. Suzuki. 2003. Influence of the DNA amount per injection on the development and EGFP expression in bovine embryos. Arch. Anim. Breeding 1:25-30.
Murakami, M., O. Perez, C.E. Ferguson, E. Behboodi, R.S. Denniston and R.A. Godke. 2003. Using in vivo-recovered oocytes and adult somatic cells from the same donor for nuclear transfer in cattle. Vet Rec. 153: (In press).
Pinto, C.R.F., D.L. Paccamonti, B.E. Eilts, C.S. Venugopalan, C.R. Short, L.R. Gentry, D.L. Thompson Jr. and R.A. Godke. 2003. Concentrations of nitric oxide in equine preovulatory follicles before and after administration of human chorionic gonadotropin. Theriogenology 60:819-827.
Pinto, C.R.F., D.L. Paccamonti, B.E. Eilts, C.R. Short , L. Gentry, D.L. Thompson and R.A.
Godke. Evidence of nitric oxide-mediated modulation of equine granulosa cell steroidogenesis. Theriogenology (Submitted).
Sansinena, M., D. Hylan, K. Hebert, R.S. Denniston and R.A. Godke. 2003. Benteng (Bos javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenology (Under review).
Sansinena, M., Paccamonti, D., C. Cochran, M. Meintjes, B. Reggio, R. S. Denniston, C. Pinto, B. Eilts and R.A. Godke. 2003. Developing the intracytoplasmic sperm injection (ICSI) procedure for the horse. In: From Epididymus to Embryo. Havemeyer Fdn. Monograph 6:69-74.
Sansinena, M., S.A. Taylor, P.J. Taylor, R.S. Denniston and R.A. Godke. 2003. Production of nuclear transfer llama (Lama glama ) embryos from in vitro fertilized llama oocytes. Cloning & Stem Cells 5(3):191-198.
Sansinena, M.J., B. Reggio, D. Hylan, A. Klumpp, S.K. Lyle, R.S. Denniston, D. Paccamonti and R.A. Godke. 2003. Effect of different pre-maturation treatments on in vitro maturation of equine oocytes and subsequent competence for nuclear transfer. Havemeyer Fdn. Monograph (In press).
Songsasen, N., I. Yu, M. Gomez and S.P. Leibo. 2003. Effects of meiosis-inhibiting-agents and equine chorionic gonadotropin on nuclear maturation of canine oocytes. Molec. Reprod. Develop. 65:435-445.
VandeVoort, C.A., S.P. Leibo and A.F.Tarantal. 2003. Improved collection and developmental competence of immature macaque oocytes. Theriogenology 59:699-707.
Yu, I., N. Songsasen, Y-J. Kim, B.L. Dresser and S.P. Leibo. 2003. Development in culture of canine ovarian and testicular fibroblasts after cryopreservation. Cryo-Letters (In press).
Wirtu, G., C.E. Pope, J. Vaccaro, E. Saart, A. Cole, R.A. Godke and B.L. Dresser. Dominance hierachy in a small herd of female eland (Taurotragus oryx) in captivity. Zoo Biol. (In press).
Wirtu, G., C.E. Pope, P. Damiani, F. Miller, B.L. Dresser, R.A. Godke and B.D. Bavister. Development of in vitro-derived bovine embryos in protein-free media: Effects of amino acids, glucose, pyruvate, lactate, phosphate and osmotic pressure. Reprod. Fertil. Develop. (Submitted).
TEXAS
Westhusin M., K. Hinrichs, Y.H. Choi, T. Shin, L. Liu and D. Kraemer. 2003. Cloning Companion Animals (Horse, Cat, Dog). Cloning and Stem cells. (in press).
Golding, M.C. and M.E. Westhusin. 2003. Analysis of DNA (cytosine-5) methyltransferase mRNA sequence and expression in preimplantation embryos, fetal and adult tissues. Gene Expression Patterns 3: 551-558.
Willingham-Rocky L.A., K. Hinrichs, M.E. Westhusin and D.C. Kraemer. 2003. Effects of stage of cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction (in press).
Choi Y.H., L.B. Love, M.E. Westhusin and K. Hinrichs. 2003. Remodling of equine somatic cell nuclei after transfer to equine or bovine cytoplasts, and effect of timing of activation treatment on in vitro development of horse nuclear transfer embryos. Biol. Reprod. (in press).
Long CR, S.C. Walker, R.T. Tang and M.E. Westhusin. 2003. New commercial opportunities for advanced reproductive technologies in horses, wildlife, and companion animals. Therio. 59:139-149.
Choi Y.H., Y.G. Chung, S. Walker, M.E. Westhusin and K Hinrichs. 2003. In vitro development of equine nuclear transfer embryos: Effects of oocyte maturation and amino acid composition during embryo culture. Zygote 11:77-86.
UTAH
Woods, G.L., K.L. White, D.K. Vanderwall, Guang-Peng Li, K.I. Aston, T.D. Bunch, L.N. Meerdo, and B.J. Pate. 2003. A mule clone from fetal cells by nuclear transfer. Science. 301: 1063.
WASHINGTON
Jiang, Zhihua, M. Zhang, V. Wasem, J. Michal, H. Zhang, R.W. Wright. 2003. Census of Genes Expressed in Porcine Embryos and Reproductive Tissues by Mining an Expressed Sequence Tag Data Base Based on Human Genes. Biol. of Reprod. 69:1177-1182.
WISCONSIN
Block, J., M. Drost, R.L. Monson, J.J. Rutledge, R. M. Rivera, F.F. Paula-Lopes, O.M. Ocon, C.E. Krininger III, J. Liu and P.J. Hansen. 2003. Use of insulin-like growth factor-1 during embryo culture and treatment of redipients with GnRH to increase pregnancy rates following the transfer of in vitro produced embryos to heat-stress, lactating cows. J. Anim. Sci. 81: 1590-1602.
Fischer-Brown, Amy and Jack Rutledge. 2003. Practical and research applications of elongation stage bovine embryos. Embryo Transfer Newsletter 21: 11-15.
Men H, Monson RL, Parrish JJ, Rutledge JJ. 2003. Degeneration of cryopreserved bovine metaphase II oocytes via apoptosis during subsequent culture. Cryobiology 47:73-81.
Men H, Monson RL, Parrish JJ. Rutledge JJ. 2003. Detection of direct DNA damage in bovine metaphase II oocytes caused by cryopreservation. Mol Reprod Dev 64:245-250.
Books, chapters (non-refereed) in books, proceedings, and theses
CANADA
Mapletoft, R.J., Colazo, M.G., Martinez, M.F. and Kastelic, K.P., 2003. Esteres de Estrogeno para la Sincronizacion de la Emergencia de la Onda Follicular y la Ovulacion en Animales Tratados con Dispositivos con Progesterona. V Simposio Internacional de Reproduccion Animal. Heuta Grande, Cordoba, Argentina, pages 55-70.
Kastelic, K.P. and Mapletoft, R.J., 2003. Causas no Infecciosas de Muerte Embrionaria en Ganado Bovino. V Simposio Internacional de Reproduccion Animal. Heuta Grande, Cordoba, Argentina, pages 149-160.
Barth, A.D., Thundathil, J.C. and Mapletoft, R.J., 2003. Importancia de la Calidad Seminal y el Uso de FIV para el Estudio de Defectos Espermaticos. V Simposio Internacional de Reproduccion Animal. Heuta Grande, Cordoba, Argentina, pages 205-222S.
Mapletoft, R.J., Martinez, M.F., Colazo, M.G. and Kastelic, J.P., 2003. The Use of Controlled Internal Drug Release Devices for the Regulation of Bovine Reproduction. Journal of Animal Science, 81(E. Suppl. 2):E28?E36.
COLORADO
Checura, C. 2003. Cryopreservation of Bovine Oocytes: Effects of Macromolecules in Vitrification Solutions. M.S. Thesis, Colorado State University, Fort Collins, CO.
DeLille, A. 2003. Physical and Molecular Characteristics of Day 75 Nuclear Transfer Cloned Bovine Conceptuses. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.
Garner, D. L., and G. E. Seidel, Jr. 2003. Applications of flow cytometry in animal reproduction. In Press in Flow Cytometry in Biotechnology, L.A. Sklar (ed). Oxford University Press (USA).
Seidel, G. E., Jr. 2003. Cloning I. Scientific background. In Press in Encyclopedia of Bioethics. S.G. Post (ed). MacMillan, New York.
Seidel, G. E., Jr. 2003. Metabolism of bovine embryos. Pages 3-8 in Proc. Ann. Conv. Am. Embryo Transfer Assoc.
Seidel, G. E., Jr. 2003. Vitrification of bovine embryos. Pages 9-12 in Proc. Ann. Conv. Am. Embryo Transfer Assoc.
Seidel, G. E., Jr. 2003. Transgenic vertebrates ? The long road to practical application. Pages 1-2 in Proc. Transgenic Animal Research Conference IV, Tahoe City, CA.
Seidel, G. E., Jr., R. P. Elsden, and J. F. Hasler. 2003. Embryo Transfer in Dairy Cattle. 3rd Edition. Hoard?s Dairyman Books, Fort Atkinson, WI. 96 pages.
Tubman, L. 2003. Characteristics of Calves Produced with Sperm Sexed by Flow Cytometry/Cell Sorting. M.S. Thesis, Colorado State University, Fort Collins, CO.
CONNECTICUT
Lee, J.W. 2003. Production of pig embryos following microinjection of somatic cells and sperm. Ph.D. thesis. University of Connecticut.
Enright, B. 2003. Development and subsequent reproductive performance of cloned cattle derived from adult somatic cells. Ph. D. thesis. University of Connecticut.
Kubota, C. 2003. Studies on the development of bovine somatic cell nuclear transfer and its application for the production of super sire bull. Ph. D. thesis. University of Kagoshima.
He, J. 2003. Quantification of extracellular matrix gene expression in single bovine oocytes and preimplantation embryos derived from IVF using real time RT-PCR. MS thesis. University of Connecticut.
ILLINOIS
Clark, Sherrie G., Ph.D., December 2003, dissertation title, "In vitro maturation, fertilization and embryo culture of porcine gametes in a microfluidic environment", University of Illinois at Urbana-Champaign, 106 pgs.
Etherton, T.D., Bauman, D.E., Beattie, C.M., Bremel ,R.D., Cromwell, G.L, Kapur, V., Varner, G., Wheeler, M.B., Wiedmann, M. (2003). Biotechnology in Animal Agriculture: An Overview. Council for Agricultural Science and Technology Issue Paper, 12 pgs.
Jackson, Kevin S., Ph.D. May 2003, dissertation title, "Trophinin: Expression and regulation in the porcine uterus and embryo during the time of embryo implantation", University of Illinois at Urbana-Champaign, 124 pgs.
Roseman, Hayley M., M.S., October 2003, thesis title. "Porcine oocyte maturation and embryo culture within polydimethylsiloxane-borosilicate microchannels" , University of Illinois at Urbana-Champaign, 92 pgs.
LOUISIANA
Fahrig, B. 2003. Cryopreservation by Pellet Freezing of Epididymal and Ejaculated Spermatozoa from Male Dogs. M.S. Thesis. Louisiana State University. Baton Rouge.
James, A.N. 2003. Preservation of Sperm Harvested from the Rat, Caprine, Equine and Bovine Epididymis. Ph.D. Dissertation. Louisiana State University. Baton Rouge.
Leibo, S.P. 2003. Basic cryobiology of embryos. In: Andrology and Embryology Review Course Manual. American Association of Bioanalysts. St. Louis, MO. 14:1-15.
Nel-Themaat, L., T. Harding, J.E. Chandler, J.F. Chenevert, P. Damiani, M.J. Fernadez and R.A. Godke. 2003. Freezability of sperm ejaculates collected multiple times weekly from endangered Louisiana Native rams. Proc Amer. Zoo Assoc. (In press).
Nel-Themaat, L., T. Harding, J.E. Chandler, J.F. Chenevert, P. Damiani, M.J. Fernadez and R.A. Godke. 2003. Freezability of endangered Louisiana Native ram sperm. Proc. Environ-mental State of State Conf. (ESOS) VIII. (In press).
Perez. O. 2003. Oocyte Production in the Postpartum Beef Cattle. Ph.D. Dissertation. Louisiana State University. Baton Rouge.
Sansinena, M. 2003. Somatic Cell Interspecies Nuclear Transfer. Ph.D. Dissertation. Department of Animal Sciences. Louisiana State University. Baton Rouge.
OREGON
Peoples, M. 2003. The effects of the phosphatase inhibitor, okadaic acid, on plasminogen activator activity in bovine oocyte-cumulus cell complexes matured in vitro. Senior Thesis, Oregon State University, Corvallis, OR.
WISCONSIN
Jensen, Nancy L. Bovine In Vitro Embryo Production using Avian White Yolk. M.S. Thesis, Department of Animal Sciences, University of Wisconsin.
Abstracts
ARKANSAS
Rorie, R.W., C.F. Rosenkrans and A.J. Aishman. 2004. Effects of bovine somatotropin treatment on the AI pregnancy rate in dairy heifers. Reprod. Fert. Dev.
CALIFORNIA
Batchelder, C.A., K.A. Hoffert, M. Bertolini, A.L. Moyer and G.B. Anderson. 2004. Development of bovine nuclear transfer embryos cloned from follicular donor cells in sequential stages of differentiation. Annual Meeting, International Embryo Transfer Society, January 12-14, 2004, Portland, OR.
Hoffert, K.A., C.A. Batchelder, M. Bertolini, A.L. Moyer and G.B. Anderson. 2004. Angiogenesis in cloned and IVF-derived bovine pregnancies at day 30 of gestation. Annual Meeting, International Embryo Transfer Society, January 12-14, 2004, Portland, OR.
McCarthy, M., E. E. At-Taras, B. J. Nitta-Oda, C. Ducummon, C. Pearl, C., J. F. Roser, and T. Berger. 2003. Reduction of endogenous estrogens reduces epididymal boar sperm fertility. Biol. Reprod. 68 (Suppl 1):301.
Khalil, M.B., K. Chakrabandhu, E. Carmona, H. Xu, N. Vuong, P. Kumarathasan, S. da Silva, D. Carrier, P. Wong, M. Buhr, T. Berger, and N. Tanphaichitr. 2003. Pig sperm rafts exhibit zona-pellucida binding ability and contain mammalian male germ cell specific sulfogalactosylglycerolipid and arylsulfatase A. Biol. Reprod. 68 (Suppl 1): 167.
Khalil, M.B., K. Chakrabandhu, P. Wong, M. Buhr, N. Vuong, J. Mayne, E. Carmona, T. Berger, P. Kumarathasan, H. Xu, S. Wong, M. Na, D. Carrier, M. Mbikay, and N. Tanphaichitr. 2003. Sperm rafts bind to the egg zona pellucida and contain sulfogalactosylglycerolipid. Mol. Biol. Cell 14 (suppl).
Mason, J.B., J. Najarian, E.A. Maga, J. Conrad-Brink, J.D. Murray and G.B. Anderson. 2003. The effect of coating single- and double-stranded DNA with the recombinase A protein of Escherichia coli on transgene integration in mice. Third Biennial Conference on Transgenic Animal in Agriculture, August 12-15, 2003, Lake Tahoe, CA.
CANADA
Colazo, M.G., Whittaker, P.R., Kastelic, J.P. and Mapletoft, R.J. 2003. CIDR-based protocols for synchronizing bovine embryo transfer recipients without estrus detection. Journal of Animal Science, 81 (Suppl. 1)/Journal of Dairy Science, 86 (Suppl. 1), 183 (Abstract).
Colazo, M.G., Gavaga, Q.A., Kastelic, J.P. and Mapletoft, R.J., 2003. The Effects of Estradiol Cypionate (ECP) at CIDR Removal and Interval to Fixed-time AI on Pregnancy Rates in Beef Cattle. Theriogenology, 59, 215. (Abstract).
Kastelic, J.P., Colazo, M.G., Martinez, M.F. and Mapletoft, R.J., 2003. Effects of a CIDR or MGA, Reproductive Status and Estradiol, GnRH or pLH Treatment on Pregnancy Rate to Fixed-time AI in Beef Heifers. Theriogenology, 59, 223. (Abstract).
Martínez, M.F., Colazo, M.G., Kastelic, J.P. and Mapletoft, R.J., 2003. Effects of Estradiol and Progesterone on Plasma Steroid and Gonadotrohin Concentrations in CIDR-treated Ovariectomized Cows. Theriogenology, 59, 224. (Abstract).
Nasser, L.F., Bo, G.A., Reis, E.L., Menegati, J.A., Marques, M.O., Mapletoft, R.J. and Baruselli, P.S., 2003. Superovulatory Response during the First Follicular Wave In Nelore (Bos Indicus) Donors. Theriogenology, 59, 530. (Abstract).
COLORADO
Bass, L .D., D. J. Denniston, L. J. Maclellan, P. M. McCue, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of methanol as a cryoprotectant for equine embryos. Proc. 19th Equine Nutr. Physiol. Soc. Symp.
Campos-Chillon, L. F., J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Two-step vitrification and in-straw dilution of in vitro produced bovine embryos. J. Anim. Sci. 81 (Suppl. 1):143 (abstr. #565).
Checura, C. M., and G. E. Seidel, Jr. 2003. Effect of macromolecules for bovine oocyte vitrification. Theriogenology 59:207.
Coutinho da Silva, M. A., E. M. Carnevale, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of semen extender and centrifugation through Percoll on binding of stallion sperm to bovine zonae pellucidae. Proc. Soc. Theriogenology. In Press.
De La Torre, J. F., and G. E. Seidel, Jr. 2003. Timing of dinitrophenol treatment during in vitro culture of bovine embryos. J. Anim. Sci. 81 (Suppl. 1):142 (abstr. #564).
De La Torre-Sanchez, J. F., and G. E. Seidel, Jr. 2003. Effects of glucose concentration and presence of EGF and hormones on bovine oocyte maturation. J. Anim. Sci. 81 (Suppl. 1):186 (abstr. #M32).
Flint, A., Z. Brink, and G. E. Seidel, Jr. 2003. Use of heterospermic insemination and genotyping embryos to compare fertility of flow-sorted sperm from individual bulls. Theriogenology 59:507.
Schenk, J. L., W. B. Henderson, and G. E. Seidel, Jr. 2003. Timed insemination of superovulated heifers with sexed sperm. J. Anim. Sci. 81 (Suppl. 1):118 (abstr. #470).
Seidel, G. E., Jr., Z. Brink, and J. L. Schenk. 2003. Use of heterospermic insemination with fetal sex as the genetic marker to study fertility of sexed sperm. Theriogenology 59:515.
Tubman, L. M., Z. Brink, T. K. Suh, and G. E. Seidel, Jr. 2003. Normality of calves resulting from sexed sperm. Theriogenology 59:517.
Walker, D. J., J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Effects of glucose concentration and presence of EGF and hormones on bovine oocyte maturation. J. Anim. Sci. 81 (Suppl. 1):33 (abstr. #132).
CONNECTICUT
Enright, B.P., X. C. Tian. 2003. Epigenetic Characteristics of Donor Cells for Nuclear Transfer: Levels of Histone Acetylation. Theriogenology 58:251.
Bormann, C. L., B. P. Enright, X. Yang. 2003. Increased parthenogenetic development of bovine oocytes activated with a combinations of phosphorylation and protein synthesis inhibitors. Theriogenology 58:471.
Jeong, B.-S., F. Xue, X. C. Tian. 2003. 5?-aza-2?-deoxycytidine induces expression of PLP1, an X-linked gene normally silent in bovine adult fibroblasts Theriogenology 58:422.
Lee, J.-W., A. Dinnyes, X. Yang. 2003. Development of transgenic embryos following injection of freeze-dried sperm in pigs. Theriogenology 58:305.
Liu, J.-L., L.-Y. Sung, F. Du, M. Julian, S. Jiang, M. Barber, J. Xu, X. C. Tian, X. Yang. 2003. Differential development of rabbit embryos derived from parthenogenesis and nuclear transfer. Theriogenology 58:271.
Nedambale, T. L., S. A. Chaubal, F. Du, W. Groen, D. C. Faber, A. Dinnyes, X. Yang. 2003. Vitrification methods for cryopreservation of in vitro produced cattle embryos. Theriogenology 58: 307.
Wang L., B.-S. Jeong, E. Duan, X. C. Tian. 2003. Effect of donor cell age on number of population doubling of feline fibroblasts cultured in vitro Theriogenology 58:535.
ILLINOIS
Bonde, S., Varzavand, A., Zou, G.-M., Field, E., Walters, E.M., Wheeler, M.B., Zavazava, N. (2003) Porcine-Derived Embryonic Stem Cells are Immune-Privileged and Resistant to Natural Killer Cell Lysis. American Journal of Transplantation (Suppl. 5), S546.
Clark, S.G. Walters, E.M. Beebe, D.J., Wheeler, M.B. (2003). A Novel Integrated In Vitro Maturation and In Vitro Fertilization System In Swine. Theriogenology 59:441.
Clark, S.G., Walters, E.M., Beebe, D. J., M.B. Wheeler. (2003). Determination of the BSA content needed for culture of porcine embryos fertilized in micro
channels. Biol. Reprod. 68 (Suppl. 1) 336.
Donovan ,S.M, Hartke, J.L., Monaco, M.H., Wheeler, M.B. (2003) Over-expression of IGF-I effects on piglet intestinal growth. J. Dairy Sci 86 (Suppl. 1): 134.
Gronlund D.E., Hurley, W.L, Monaco, M.H., Wheeler, M.B., Donovan, S.M. (2003) Over-expression of insulin-like growth factor-I in lactating porcine mammary tissue has a differential effect on amino acid transport systems. J. Dairy Sci 86 (Suppl. 1): 190.
Hartke J.L., Monaco, M.H., Wheeler, M.B., Donovan, S.M. (2003) Effect of a short-term fast on intestinal disaccharidase activity and villus morphology in piglets suckling insulin-like growth factor-I (IGF-I) transgenic sows. J. Dairy Sci 86 (Suppl. 1): 134.
Hartke J.L., Monaco, M.H., Wheeler, M.B., Donovan, S.M. (2003) Intestinal growth and development in piglets suckling insulin like growth factor-I (IGF-I) transgenic sows. J. Dairy Sci.86 (Suppl. 1): 311.
Marshall, K.M., Shanks, R.D., Wheeler, M.B. (2003) Increased nursing intensity stimulated greater lactational response in sows expressing a bovine alpha-lactalbumin transgene. Biol. Reprod. 68 (Suppl. 1) 202.
Monaco, M.H., Hurley, W.L, Wheeler, M.B., Donovan, S.M. (2003) Insulin-like growth factor-I (IGF-I) modulates the process of mammary apoptosis after weaning in IGF-I transgenic pigs. J. Dairy Sci 86 (Suppl. 1): 301.
Walters, E.M., Clark, S.G, Roseman, H., Beebe, D.J., Wheeler, M.B. 2003. Production of Live Piglets Following In Vitro Embryo Culture in Microfluidic Environment. Theriogenology 59:353.
LOUISIANA
Barry, D.M., G. Herholdt, A. Visser, B.B. Hzimhanovich and R.A. Godke. 2003. Development of mammalian embryos and stem cells at micro-gravity conditions at the international space station. Theriogenology 59:337.
Damiani, P., G. Wirtu, F. Miller, C.E. Pope, A. Cole, R.A. Godke and B.L. Dresser. 2003. Development of Giant eland antelope (Taurotragus derbianus) embryos following nuclear transfer with Common eland (Taurotragus oryx) and bovine (Bos taurus) oocytes. Theriogenology 59:390.
Ferguson, C.E., D.J. Kesler, T.R. Davidson, B. Lenard and R.A. Godke. Saving pregnancies in beef cattle after a luteolytic dose of prostaglandin. Theriogenology (In press).
Godke, R.A., Sansinena,M.J., B. Reggio, D. Hylan, A. Klumpp, S.K. Lyle, R.S. Denniston, and D. Paccamonti. 2003. Effect of different pre-maturation treatments on in vitro maturation of equine oocytes and subsequent competence for nuclear transfer. Proc. Havemeyer Workshop. p.13.
Godke,R.A., K. Hebert, A. James, R.S. Denniston, D. Paccamonti. 2003. The potential use of epididymal sperm from bulls and stallions for assisted fertilization. Proc. Havemeyer Workshop. p. 21.
Gómez, M.C., C.E. Pope, A.M. Giraldo, L. Lyons, R.F. Harris, A. King, A. Cole, R.A. Godke and B.L. Dresser. Birth of African Wildcat cloned kittens. Theriogenology (In press).
Klump, A, Aljarrah A, Hylan D, Pinto C, Sansinena M, Eilts B, Godke RA and Paccamonti D. 2003. Follicular aspiration during transition to advance the onset of cyclicity. Proc. Leipzig Expert Workshop on Equine Reproductive Medicine (Abstract).
Klumpp, A. M., R.S. Denniston D. Paccamonti, S.P. Leibo, and R.A. Godke. Embryonic development after holding bovine oocytes in undiluted follicular fluid for a 6-hour prematuration period. Theriogenology (In press).
Landry, A.M., Y. Echelard, M. Murakami, M.G. Collins, R.S. Denniston and R.A. Godke. 2003
Effect of electrofusion method and time of cleavage on bovine embryo development after blastomere fusion at the 2-cell stage. Theriogenology 59:355.
Moisan, A.E., J.W. Lynn, S.P. Leibo, R.S. Denniston and R.A. Godke. Volumetric changes of bovine oocytes resulting from dehydration in disaccharides solution. Theriogenology (In press).
Nel, L., R.A. Godke, B.L. Dresser and P. Damiani. Isolation, culture and cryopreservation of somatic cells obtained from fresh and cooled semen, and fresh milk of gulf coast native sheep. Theriogenology (In press).
Sansinena, M.J. Hylan, D., Klump, A., Reggio, B, Denniston, R.S. and Godke, R.A. 2003. Effect of roscovitine prematuration on in vitro maturation of equine oocytes from small ovarian follicles and subsequent nuclear transfer efficiency. Proc World Equine Vet. Med. Assoc. (Abstract).
Sansinena ,M., P. Taylor, S. Taylor, R.S. Denniston and R.A. Godke. 2003. Production of interspecies nuclear transfer embryos using male and female llama (Lama glama) cell lines. Theriogenology 59:287.
Wirtu, G., P. Damiani, B. Bavister, F. Miller, B. Dresser, R. Godke and E. Pope. 2003. Effects of eland and bovine serum on the development of in vitro-derived bovine embryos. Theriogenology 59:354.
Wirtu, W., C. E. Pope, P. Damiani, B.L. Dresser, R.A. Godke and B.D. Bavister. Insulin improves frequency of cleavage and subsequent development of bovine embryos in vitro. Theriogenology (In press).
WISCONSIN
Fischer-Brown A., D. Northey, J. Parrish, and J. Rutledge 2003. Post-transfer growth of in vitro-produced bovine embryos. Biol Reprod 68 (Supp 1):252.
Fischer-Brown A., R. Monson, D. Northey, J. Rutledge, K. Albrecht, and D. Schaefer 2003. Reduced dietary protein improves pregnancy rates following transfer of in vitro produced bovine embryos. Theriogenology 59(1):364.
d. Miscellaneous publications
CANADA
Martinez, M.F., Adams, G.P., Kastelic, J.P. and Mapletoft, R.J., 2001. Artificial Insemination without Heat Detection in Beef Heifers. Large Animal Veterinary Rounds, 1, 2.
Mapletoft, R.J., Schmutz, S.M. and Searcy, G.P., 2000. Observations on the Inheritance of a Bleeding Disorder in Simmental Cattle. Simmental Country, 21, 18.
Mapletoft, R.J., Barth, A.D., Kastelic, J.P. and Coulter, G.H., 1999. Fertility and Puberty in Young Beef Bulls. Simmental Country, February 1999, 80-81.
LOUISIANA
Ferguson, C.F., O. Perez, K. Hebert , J. Cartmill and R.A. Godke. 2003. Using altrenogest to synchronize estrus in beef heifers. LSU AgCenter Beef Cattle Research Report 32:55-56.
Hebert, K., D. Hylan, R.S. Denniston and R.A Godke. 2003. Administering prostaglandin F2a twice daily for 5 days to nursing beef cows starting 5 days after calving. LSU AgCenter Beef Cattle Research Report 32:58-59.
Godke, R.A. and R.S. Denniston. 2003. Biotechnology and Cattle Production in the Future. OvaGenix Information Day Report 1:47-53.
Godke, R.A., R.S. Denniston and B. Reggio. 2003. Animal biotechnology and the future. Louisiana Agric. pp. 10-14.
IOWA
Youngs, C.R. 2003. Use of gonadotropin releasing hormone for controlled breeding of dairy cattle. Iowa Jersey Today 9(1):9-10.
ARKANSAS
Baublits, R.T., A.H. Brown, Jr., Z.B. Johnson, C.F. Rosenkerans, Jr., R.W. Rorie and B.A. Sandelin. 2003. Variation in the percentage of female births over time in five breeds of registered cattle. Prof. Anim. Sci. 19:204-210.
Steelman, C.D., R.W. McNew, R.B. Simpson, R.W. Rorie, J.M. Phillips and C.F. Rosenkrans, Jr. 2003. Evaluation fo alternative tactics for management of insecticide-resistant horn flies (diptera muscidae). J. Econ. Entomol. 96:892-901.
CALIFORNIA
Bertolini, M., J.B. Mason, S.E. Beam, G.F. Carneiro, M.L. Sween, D.J. Kominek, A.L. Moyer, T.R. Famula, R.D. Sainz and G.B. Anderson. 2002. Morphology and morphometry of in vitro-and in vitro-produced concepti from early pregnancy to term and association with high birth weights. Theriogenology 58:973-994.
Bertolini, M., S.E. Beam, H. Shim, L.R. Bertolini, A.L. Moyer, T.R. Famula and G.B. Anderson. 2002. Growth, development and gene expression by in vitro-and in vitro-produced day-7 and day-16 bovine embryos. Molecular Reproduction and Development 63:318-328.
Carmona, E., W. Weerachatyanukul, T. Soboloff, A. L. Fluharty, D. White, L. Promdee, M. Ekker, T. Berger, M. Buhr, and N. Tanphaichitr. 2002. Arylsulfatase A is present on the pig sperm surface and is involved in sperm-zona pellucida binding. Dev. Biol. 247:182.
Sartini, B.L. and T. Berger. 2003. Changes in oocyte plasma membrane binding sites on boar spermatozoa with capacitation and acrosome reactions. Reproduction 125:865.
Rui, R., H. Shim, A.L. Moyer, D. Anderson, M.C. Penedo, J.D. Rowe, R.H. BonDurant and G.B. Anderson. Attempts to enhance production of chimeras from porcine embryonic germ cells and preimplantation porcine embryos. Theriogenology (In press).
Maga, E.A., G. Sargent, H. Zeng, S. Pati, D. Zarling, S. M. Oppenheim, N.M.B. Collette, A.L. Moyer, J.S. Conrad-Brink, J. D. Rowe, R.H. BonDurant, G.B. Anderson, and J.D. Murray. Increased efficiency of transgenic livestock production. Transgenic Reseach (In press).
CANADA
Bo, G.A. and Mapletoft, R.J., 2003. Superovulacion en Bovinos. En: Reproduccion en los Animales Domisticos. Tomo II. R. Ungerfeld (Editor). Melibea Ediciones, pages 515-538.
Bo, G.A., Palasz, A.T. and Mapletoft, R.J., 2003. Criopreservacion de Embriones. En: Reproduccion en los Animales Domisticos. Tomo II. R. Ungerfeld (Editor). Melibea Ediciones, pages 551-566.
Vassena, Rita, Mapletoft, Reuben J., Allodi, Stefano, Singh, Jaswant, Adams, Gregg P., 2003. Morphology and Developmental Competence of Bovine Oocytes relative to Follicular Status. Theriogenology, 60, 923-932.
Colazo, Marcos German, Kastelic, John Patrick, Mapletoft, Reuben John., 2003. Effects of Estradiol Cypionate (ECP) on Ovarian Follicular Dynamics, Synchrony of Ovulation and Fertility in CIDR-based, Fixed-time AI Programs in Beef Heifers. Theriogenology, 60, 855-865.
Martinez, M.F., Mapletoft, R.J., Kastelic, J.P. and Carruthers, T.D., 2003. The Effects of Three Gonadorelin Products on Luteinizing Hormone Release, Ovulation, and Follicular Wave Emergence in Cattle. Canadian Veterinary Journal, 44:125-131.
Vassena, R., Adams, G.P., Mapletoft, R.J., Pierson, R.A. and Singh, J, 2003. Ultrasound Image Characteristics of Ovarian Follicles in Relation to Oocyte Competence and Follicular Status in Cattle. Animal Reproduction Science, 76, 25-41.
COLORADO
Bass, L. D., D. J. Denniston, L. J. Maclellan, P. M. McCue, G. E. Seidel, Jr., and E. L. Squires. 2003. Methanol as a cryoprotectant for equine embryos. Theriogenology. Submitted.
Choi, Y. H., F. C. Landim-Alvarenga, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of capacitation of stallion sperm with polyvinylalcohol or bovine serum albumin on penetration of bovine zona-free or partially zona-removed equine oocytes. J. Anim. Sci. 81:2080-2087.
Coutinho da Silva, M. A., E. M. Carnevale, L. J. Maclellan, K. A. Preis, G. E. Seidel, Jr., and E. L. Squires. 2003. Oocyte transfer in mares with intrauterine or intraoviductal insemination using fresh, cooled and frozen stallion semen. Theriogenology. In Press.
Flint, A. F., P. L. Chapman, and G. E. Seidel, Jr. 2003. Heterospermic insemination of cattle using flow-sorted sperm. J. Anim. Sci. 81:1814-1822.
Franz, L. C., Y.-H. Choi, E. L. Squires, G. E. Seidel, Jr., and K. Hinrichs. 2003. Effects of roscovitine on maintenance of the germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection. Reproduction 125:693-700.
Garner, D. L., and G. E. Seidel, Jr. 2003. Past, present and future perspectives on sexing sperm. Can. J. Anim. Sci. 83:375-384.
Glover, M. D., and G. E. Seidel, Jr. 2003. Increased messenger RNA for allograft inflammatory factor-1, LERK-5, and a novel gene in 17.5-day relative to 15.5-day bovine embryos. Biol. Reprod. 69:1002-1012.
Li, G.-P., G. E. Seidel, Jr., and E. L. Squires. 2003. Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa. Theriogenology 59:1143-1155.
Li, G.-P., G. E. Seidel, Jr., and E. L. Squires. 2003. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology. In Press.
Lindsey, A. C., D. D. Varner, G. E. Seidel, Jr., J. E. Bruemmer, and E. L. Squires. 2003. Hysteroscopic or rectally guided, deep-uterine insemination of mares with spermatozoa stored at 18 h at either 5ºC or 15ºC prior to flow-cytometric sorting. Anim. Reprod. Sci. Submitted.
Mendes, J. O. B., Jr., P. D. Burns, J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Effect of heparin cleavage rates and embryo production with four bovine sperm preparation protocols. Theriogenology 60:331-340.
Seidel, G. E., Jr., 2003. Economics of selecting for sex: The most important genetic trait. Theriogenology 59:585-598.
Seidel, G. E., Jr. 2003. Sexing mammalian sperm ? Intertwining of commerce, technology, and biology. Anim Reprod Sci. In Press.
Zhang, M., K. H. Lu, and G. E. Seidel, Jr. 2003. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically-sorted, stained, and unsorted sperm from different bulls. Theriogenology 60:1657-1663.
CONNECTICUT
Enright, B. P., B. S. Jeong, X. Yang, and X. C. Tian. 2003a. Epigenetic characteristics of bovine donor cells for nuclear transfer: Levels of histone acetylation. Biol Reprod 69: 1525-1530.
Enright, B. P., C. Kubota, X. Yang, and X. C. Tian. 2003b. Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin a or 5-aza-2‘‘‘‘-deoxycytidine. Biol Reprod 69: 896-901.
Lee, J. W., X. C. Tian, and X. Yang. 2003a. Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection. Biol Reprod 68: 1341-1347.
Lee, J. W. et al. 2003b. Production of cloned pigs by whole-cell intracytoplasmic microinjection. Biol Reprod 69: 995-1001.
Savage, A. F. et al. 2003. Behavioral observations of adolescent holstein heifers cloned from adult somatic cells. Theriogenology 60: 1097-1110.
Suzuki, H., Y. Saito, N. Kagawa, and X. Yang. 2003. In vitro fertilization and polyspermy in the pig: Factors affecting fertilization rates and cytoskeletal reorganization of the oocyte. Microsc Res Tech 61: 327-334.
Tian, X. C., C. Kubota, B. Enright, and X. Yang. 2003. Cloning animals by somatic cell nuclear transfer - biological factors. Reprod Biol Endocrinol 1: 98.
Xu, J., and X. Yang. 2003. Will cloned animals suffer premature aging - the story at the end of clones‘‘‘‘ chromosomes. Reprod Biol Endocrinol 1: 105.
Yang, X. 2003. Toward therapeutic cloning and regenerative biology research forum: To clone or not to clone. Reprod Biol Endocrinol 1: 97.
ILLINOIS
Wheeler, M.B., Walters, E.M., Clark, S.G. (2003). Transgenic animals in biomedicine and agriculture:outlook for the future. Animal Reproduction Science 79:265-289.
Wheeler, M.B., Malusky, S.A. (2003). Transgenesis in mammals: status and perspectives. Acta Scientiae Veterinariae 31:119-136.
IOWA
Godke, R.A. and C.R. Youngs. 2003. Biotechnology: Embryo Transfer Technology. In (W. Pond, Ed.) Encyclopedia of Animal Science. Marcel Dekker, New York, NY (in press).
LOUISIANNA
Boediono, A., T. Suzuki and R.A. Godke. 2003. Comparison of hybrid and prurebred in vitro-derived cattle embryos during in vitro culture. Anim. Reprod. Sci. 78:1-11.
Cochran, R., M. Meintjes, D. Hylan, M. Sansinena, R. S. Denniston D. Paccamonti and R.A. Godke. 2003. Follicle growth in mares for oocyte production. In: From Epididymus to Embryo. Havemeyer Fdn. Monograph 6:51-54.
Devireddy,R.V., B. Fahrig, R.A. Godke and S.P. Leibo. The effect of extracellular ice and glycerol on boar sperm water transport parameters: Convergence of theoretical and empirical rates of optimal freezing. Molec. Reprod. Develop. (In press).
Godke, R.A., K. Hebert, A. James, R.S. Denniston and D. Paccamonti. 2003. The potential use of epididymal sperm from bulls and stallions for assisted fertilization. Havemeyer Fdn. Monograph (In press).
Godke, R.A. and C. R. Youngs. Biotechnology: Embryo technology in cattle. In: Encyclopedia of Animal Science. W.G. Pond & A.W. Bell (eds.). Marcel Dekker:New York (In press). (Book Chapter)
Gomez, M., C.E. Pope, A. Giraldo, L. Lyons, R. Harris, A. King, A. Cole, R. Godke and B. Dresser. 2003. Cloned African Wildcats born from a domestic cat. Cloning & Stem Cells. (Submitted).
Han, Y., Y. Han, R.S. Denniston, A. Boediono, C. Ziomek and Godke. Development of IVF-derived caprine embryos with four different somatic cell co-cultures. Small Ruminant Res. (Accepted).
Klump, A, A. Aljarrah, M., Sansinena M., D. Hylan, C. Pinto, B. Eilts, R. Godke and D. Paccamonti. 2003. Follicular aspiration during transition to advance the onset of cyclicity. Pferdeheilkunde Equine Vet. J. 19(6):609-610.
Landry, A.M., D.J. Landry, L.R. Gentry, H.L. Green, K.L. Koonce, Y. Echlard and R.A. Godke. Endocrine profiles and growth patterns of goats produced by nuclear transfer. Cloning & Stem Cells (In Final Prepartion).
Leibo, S.P. and N. Lane. The early history of gamete cryobiology. In: Life in the Frozen State. E. Benson, B. Fuller & N. Lane (eds.). Taylor & Francis:London. (In press). (Book Chapter)
Luster, S.M., M. Murakami, S.P. Leibo, R.S. Denniston Y. Echelard, and R.A. Godke. Pregnancies resulting from nuclear transfer embryos derived from vitrified bovine oocytes. Cryobiology (In press).
Massip A, Leibo SP, Blesbois E. Cryobiology of gametes and the breeding of domestic animals. In: Life in the Frozen State. E. Benson, B. Fuller & N. Lane (eds.) Taylor & Francis:London (In press). (Book Chapter)
Murakami, M., T.R. Davidson, C.E. Ferguson, Y. Echelard and R. A. Godke. Use of the amniotic cavity of the developing chick embryo for culturing bovine 8-cell embryos derived from somatic cell nuclear transfer. Molec. Reprod. Develop. (In press).
Murakami, M., S. Ideguchi, M. Fahrudin, T. Otoi, R.A. Godke and T. Suzuki. 2003. Influence of the DNA amount per injection on the development and EGFP expression in bovine embryos. Arch. Anim. Breeding 1:25-30.
Murakami, M., O. Perez, C.E. Ferguson, E. Behboodi, R.S. Denniston and R.A. Godke. 2003. Using in vivo-recovered oocytes and adult somatic cells from the same donor for nuclear transfer in cattle. Vet Rec. 153: (In press).
Pinto, C.R.F., D.L. Paccamonti, B.E. Eilts, C.S. Venugopalan, C.R. Short, L.R. Gentry, D.L. Thompson Jr. and R.A. Godke. 2003. Concentrations of nitric oxide in equine preovulatory follicles before and after administration of human chorionic gonadotropin. Theriogenology 60:819-827.
Pinto, C.R.F., D.L. Paccamonti, B.E. Eilts, C.R. Short , L. Gentry, D.L. Thompson and R.A.
Godke. Evidence of nitric oxide-mediated modulation of equine granulosa cell steroidogenesis. Theriogenology (Submitted).
Sansinena, M., D. Hylan, K. Hebert, R.S. Denniston and R.A. Godke. 2003. Benteng (Bos javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenology (Under review).
Sansinena, M., Paccamonti, D., C. Cochran, M. Meintjes, B. Reggio, R. S. Denniston, C. Pinto, B. Eilts and R.A. Godke. 2003. Developing the intracytoplasmic sperm injection (ICSI) procedure for the horse. In: From Epididymus to Embryo. Havemeyer Fdn. Monograph 6:69-74.
Sansinena, M., S.A. Taylor, P.J. Taylor, R.S. Denniston and R.A. Godke. 2003. Production of nuclear transfer llama (Lama glama ) embryos from in vitro fertilized llama oocytes. Cloning & Stem Cells 5(3):191-198.
Sansinena, M.J., B. Reggio, D. Hylan, A. Klumpp, S.K. Lyle, R.S. Denniston, D. Paccamonti and R.A. Godke. 2003. Effect of different pre-maturation treatments on in vitro maturation of equine oocytes and subsequent competence for nuclear transfer. Havemeyer Fdn. Monograph (In press).
Songsasen, N., I. Yu, M. Gomez and S.P. Leibo. 2003. Effects of meiosis-inhibiting-agents and equine chorionic gonadotropin on nuclear maturation of canine oocytes. Molec. Reprod. Develop. 65:435-445.
VandeVoort, C.A., S.P. Leibo and A.F.Tarantal. 2003. Improved collection and developmental competence of immature macaque oocytes. Theriogenology 59:699-707.
Yu, I., N. Songsasen, Y-J. Kim, B.L. Dresser and S.P. Leibo. 2003. Development in culture of canine ovarian and testicular fibroblasts after cryopreservation. Cryo-Letters (In press).
Wirtu, G., C.E. Pope, J. Vaccaro, E. Saart, A. Cole, R.A. Godke and B.L. Dresser. Dominance hierachy in a small herd of female eland (Taurotragus oryx) in captivity. Zoo Biol. (In press).
Wirtu, G., C.E. Pope, P. Damiani, F. Miller, B.L. Dresser, R.A. Godke and B.D. Bavister. Development of in vitro-derived bovine embryos in protein-free media: Effects of amino acids, glucose, pyruvate, lactate, phosphate and osmotic pressure. Reprod. Fertil. Develop. (Submitted).
TEXAS
Westhusin M., K. Hinrichs, Y.H. Choi, T. Shin, L. Liu and D. Kraemer. 2003. Cloning Companion Animals (Horse, Cat, Dog). Cloning and Stem cells. (in press).
Golding, M.C. and M.E. Westhusin. 2003. Analysis of DNA (cytosine-5) methyltransferase mRNA sequence and expression in preimplantation embryos, fetal and adult tissues. Gene Expression Patterns 3: 551-558.
Willingham-Rocky L.A., K. Hinrichs, M.E. Westhusin and D.C. Kraemer. 2003. Effects of stage of cycle and progesterone supplementation during culture on maturation of canine oocytes in vitro. Reproduction (in press).
Choi Y.H., L.B. Love, M.E. Westhusin and K. Hinrichs. 2003. Remodling of equine somatic cell nuclei after transfer to equine or bovine cytoplasts, and effect of timing of activation treatment on in vitro development of horse nuclear transfer embryos. Biol. Reprod. (in press).
Long CR, S.C. Walker, R.T. Tang and M.E. Westhusin. 2003. New commercial opportunities for advanced reproductive technologies in horses, wildlife, and companion animals. Therio. 59:139-149.
Choi Y.H., Y.G. Chung, S. Walker, M.E. Westhusin and K Hinrichs. 2003. In vitro development of equine nuclear transfer embryos: Effects of oocyte maturation and amino acid composition during embryo culture. Zygote 11:77-86.
UTAH
Woods, G.L., K.L. White, D.K. Vanderwall, Guang-Peng Li, K.I. Aston, T.D. Bunch, L.N. Meerdo, and B.J. Pate. 2003. A mule clone from fetal cells by nuclear transfer. Science. 301: 1063.
WASHINGTON
Jiang, Zhihua, M. Zhang, V. Wasem, J. Michal, H. Zhang, R.W. Wright. 2003. Census of Genes Expressed in Porcine Embryos and Reproductive Tissues by Mining an Expressed Sequence Tag Data Base Based on Human Genes. Biol. of Reprod. 69:1177-1182.
WISCONSIN
Block, J., M. Drost, R.L. Monson, J.J. Rutledge, R. M. Rivera, F.F. Paula-Lopes, O.M. Ocon, C.E. Krininger III, J. Liu and P.J. Hansen. 2003. Use of insulin-like growth factor-1 during embryo culture and treatment of redipients with GnRH to increase pregnancy rates following the transfer of in vitro produced embryos to heat-stress, lactating cows. J. Anim. Sci. 81: 1590-1602.
Fischer-Brown, Amy and Jack Rutledge. 2003. Practical and research applications of elongation stage bovine embryos. Embryo Transfer Newsletter 21: 11-15.
Men H, Monson RL, Parrish JJ, Rutledge JJ. 2003. Degeneration of cryopreserved bovine metaphase II oocytes via apoptosis during subsequent culture. Cryobiology 47:73-81.
Men H, Monson RL, Parrish JJ. Rutledge JJ. 2003. Detection of direct DNA damage in bovine metaphase II oocytes caused by cryopreservation. Mol Reprod Dev 64:245-250.
Books, chapters (non-refereed) in books, proceedings, and theses
CANADA
Mapletoft, R.J., Colazo, M.G., Martinez, M.F. and Kastelic, K.P., 2003. Esteres de Estrogeno para la Sincronizacion de la Emergencia de la Onda Follicular y la Ovulacion en Animales Tratados con Dispositivos con Progesterona. V Simposio Internacional de Reproduccion Animal. Heuta Grande, Cordoba, Argentina, pages 55-70.
Kastelic, K.P. and Mapletoft, R.J., 2003. Causas no Infecciosas de Muerte Embrionaria en Ganado Bovino. V Simposio Internacional de Reproduccion Animal. Heuta Grande, Cordoba, Argentina, pages 149-160.
Barth, A.D., Thundathil, J.C. and Mapletoft, R.J., 2003. Importancia de la Calidad Seminal y el Uso de FIV para el Estudio de Defectos Espermaticos. V Simposio Internacional de Reproduccion Animal. Heuta Grande, Cordoba, Argentina, pages 205-222S.
Mapletoft, R.J., Martinez, M.F., Colazo, M.G. and Kastelic, J.P., 2003. The Use of Controlled Internal Drug Release Devices for the Regulation of Bovine Reproduction. Journal of Animal Science, 81(E. Suppl. 2):E28?E36.
COLORADO
Checura, C. 2003. Cryopreservation of Bovine Oocytes: Effects of Macromolecules in Vitrification Solutions. M.S. Thesis, Colorado State University, Fort Collins, CO.
DeLille, A. 2003. Physical and Molecular Characteristics of Day 75 Nuclear Transfer Cloned Bovine Conceptuses. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.
Garner, D. L., and G. E. Seidel, Jr. 2003. Applications of flow cytometry in animal reproduction. In Press in Flow Cytometry in Biotechnology, L.A. Sklar (ed). Oxford University Press (USA).
Seidel, G. E., Jr. 2003. Cloning I. Scientific background. In Press in Encyclopedia of Bioethics. S.G. Post (ed). MacMillan, New York.
Seidel, G. E., Jr. 2003. Metabolism of bovine embryos. Pages 3-8 in Proc. Ann. Conv. Am. Embryo Transfer Assoc.
Seidel, G. E., Jr. 2003. Vitrification of bovine embryos. Pages 9-12 in Proc. Ann. Conv. Am. Embryo Transfer Assoc.
Seidel, G. E., Jr. 2003. Transgenic vertebrates ? The long road to practical application. Pages 1-2 in Proc. Transgenic Animal Research Conference IV, Tahoe City, CA.
Seidel, G. E., Jr., R. P. Elsden, and J. F. Hasler. 2003. Embryo Transfer in Dairy Cattle. 3rd Edition. Hoard?s Dairyman Books, Fort Atkinson, WI. 96 pages.
Tubman, L. 2003. Characteristics of Calves Produced with Sperm Sexed by Flow Cytometry/Cell Sorting. M.S. Thesis, Colorado State University, Fort Collins, CO.
CONNECTICUT
Lee, J.W. 2003. Production of pig embryos following microinjection of somatic cells and sperm. Ph.D. thesis. University of Connecticut.
Enright, B. 2003. Development and subsequent reproductive performance of cloned cattle derived from adult somatic cells. Ph. D. thesis. University of Connecticut.
Kubota, C. 2003. Studies on the development of bovine somatic cell nuclear transfer and its application for the production of super sire bull. Ph. D. thesis. University of Kagoshima.
He, J. 2003. Quantification of extracellular matrix gene expression in single bovine oocytes and preimplantation embryos derived from IVF using real time RT-PCR. MS thesis. University of Connecticut.
ILLINOIS
Clark, Sherrie G., Ph.D., December 2003, dissertation title, "In vitro maturation, fertilization and embryo culture of porcine gametes in a microfluidic environment", University of Illinois at Urbana-Champaign, 106 pgs.
Etherton, T.D., Bauman, D.E., Beattie, C.M., Bremel ,R.D., Cromwell, G.L, Kapur, V., Varner, G., Wheeler, M.B., Wiedmann, M. (2003). Biotechnology in Animal Agriculture: An Overview. Council for Agricultural Science and Technology Issue Paper, 12 pgs.
Jackson, Kevin S., Ph.D. May 2003, dissertation title, "Trophinin: Expression and regulation in the porcine uterus and embryo during the time of embryo implantation", University of Illinois at Urbana-Champaign, 124 pgs.
Roseman, Hayley M., M.S., October 2003, thesis title. "Porcine oocyte maturation and embryo culture within polydimethylsiloxane-borosilicate microchannels" , University of Illinois at Urbana-Champaign, 92 pgs.
LOUISIANA
Fahrig, B. 2003. Cryopreservation by Pellet Freezing of Epididymal and Ejaculated Spermatozoa from Male Dogs. M.S. Thesis. Louisiana State University. Baton Rouge.
James, A.N. 2003. Preservation of Sperm Harvested from the Rat, Caprine, Equine and Bovine Epididymis. Ph.D. Dissertation. Louisiana State University. Baton Rouge.
Leibo, S.P. 2003. Basic cryobiology of embryos. In: Andrology and Embryology Review Course Manual. American Association of Bioanalysts. St. Louis, MO. 14:1-15.
Nel-Themaat, L., T. Harding, J.E. Chandler, J.F. Chenevert, P. Damiani, M.J. Fernadez and R.A. Godke. 2003. Freezability of sperm ejaculates collected multiple times weekly from endangered Louisiana Native rams. Proc Amer. Zoo Assoc. (In press).
Nel-Themaat, L., T. Harding, J.E. Chandler, J.F. Chenevert, P. Damiani, M.J. Fernadez and R.A. Godke. 2003. Freezability of endangered Louisiana Native ram sperm. Proc. Environ-mental State of State Conf. (ESOS) VIII. (In press).
Perez. O. 2003. Oocyte Production in the Postpartum Beef Cattle. Ph.D. Dissertation. Louisiana State University. Baton Rouge.
Sansinena, M. 2003. Somatic Cell Interspecies Nuclear Transfer. Ph.D. Dissertation. Department of Animal Sciences. Louisiana State University. Baton Rouge.
OREGON
Peoples, M. 2003. The effects of the phosphatase inhibitor, okadaic acid, on plasminogen activator activity in bovine oocyte-cumulus cell complexes matured in vitro. Senior Thesis, Oregon State University, Corvallis, OR.
WISCONSIN
Jensen, Nancy L. Bovine In Vitro Embryo Production using Avian White Yolk. M.S. Thesis, Department of Animal Sciences, University of Wisconsin.
Abstracts
ARKANSAS
Rorie, R.W., C.F. Rosenkrans and A.J. Aishman. 2004. Effects of bovine somatotropin treatment on the AI pregnancy rate in dairy heifers. Reprod. Fert. Dev.
CALIFORNIA
Batchelder, C.A., K.A. Hoffert, M. Bertolini, A.L. Moyer and G.B. Anderson. 2004. Development of bovine nuclear transfer embryos cloned from follicular donor cells in sequential stages of differentiation. Annual Meeting, International Embryo Transfer Society, January 12-14, 2004, Portland, OR.
Hoffert, K.A., C.A. Batchelder, M. Bertolini, A.L. Moyer and G.B. Anderson. 2004. Angiogenesis in cloned and IVF-derived bovine pregnancies at day 30 of gestation. Annual Meeting, International Embryo Transfer Society, January 12-14, 2004, Portland, OR.
McCarthy, M., E. E. At-Taras, B. J. Nitta-Oda, C. Ducummon, C. Pearl, C., J. F. Roser, and T. Berger. 2003. Reduction of endogenous estrogens reduces epididymal boar sperm fertility. Biol. Reprod. 68 (Suppl 1):301.
Khalil, M.B., K. Chakrabandhu, E. Carmona, H. Xu, N. Vuong, P. Kumarathasan, S. da Silva, D. Carrier, P. Wong, M. Buhr, T. Berger, and N. Tanphaichitr. 2003. Pig sperm rafts exhibit zona-pellucida binding ability and contain mammalian male germ cell specific sulfogalactosylglycerolipid and arylsulfatase A. Biol. Reprod. 68 (Suppl 1): 167.
Khalil, M.B., K. Chakrabandhu, P. Wong, M. Buhr, N. Vuong, J. Mayne, E. Carmona, T. Berger, P. Kumarathasan, H. Xu, S. Wong, M. Na, D. Carrier, M. Mbikay, and N. Tanphaichitr. 2003. Sperm rafts bind to the egg zona pellucida and contain sulfogalactosylglycerolipid. Mol. Biol. Cell 14 (suppl).
Mason, J.B., J. Najarian, E.A. Maga, J. Conrad-Brink, J.D. Murray and G.B. Anderson. 2003. The effect of coating single- and double-stranded DNA with the recombinase A protein of Escherichia coli on transgene integration in mice. Third Biennial Conference on Transgenic Animal in Agriculture, August 12-15, 2003, Lake Tahoe, CA.
CANADA
Colazo, M.G., Whittaker, P.R., Kastelic, J.P. and Mapletoft, R.J. 2003. CIDR-based protocols for synchronizing bovine embryo transfer recipients without estrus detection. Journal of Animal Science, 81 (Suppl. 1)/Journal of Dairy Science, 86 (Suppl. 1), 183 (Abstract).
Colazo, M.G., Gavaga, Q.A., Kastelic, J.P. and Mapletoft, R.J., 2003. The Effects of Estradiol Cypionate (ECP) at CIDR Removal and Interval to Fixed-time AI on Pregnancy Rates in Beef Cattle. Theriogenology, 59, 215. (Abstract).
Kastelic, J.P., Colazo, M.G., Martinez, M.F. and Mapletoft, R.J., 2003. Effects of a CIDR or MGA, Reproductive Status and Estradiol, GnRH or pLH Treatment on Pregnancy Rate to Fixed-time AI in Beef Heifers. Theriogenology, 59, 223. (Abstract).
Martínez, M.F., Colazo, M.G., Kastelic, J.P. and Mapletoft, R.J., 2003. Effects of Estradiol and Progesterone on Plasma Steroid and Gonadotrohin Concentrations in CIDR-treated Ovariectomized Cows. Theriogenology, 59, 224. (Abstract).
Nasser, L.F., Bo, G.A., Reis, E.L., Menegati, J.A., Marques, M.O., Mapletoft, R.J. and Baruselli, P.S., 2003. Superovulatory Response during the First Follicular Wave In Nelore (Bos Indicus) Donors. Theriogenology, 59, 530. (Abstract).
COLORADO
Bass, L .D., D. J. Denniston, L. J. Maclellan, P. M. McCue, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of methanol as a cryoprotectant for equine embryos. Proc. 19th Equine Nutr. Physiol. Soc. Symp.
Campos-Chillon, L. F., J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Two-step vitrification and in-straw dilution of in vitro produced bovine embryos. J. Anim. Sci. 81 (Suppl. 1):143 (abstr. #565).
Checura, C. M., and G. E. Seidel, Jr. 2003. Effect of macromolecules for bovine oocyte vitrification. Theriogenology 59:207.
Coutinho da Silva, M. A., E. M. Carnevale, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of semen extender and centrifugation through Percoll on binding of stallion sperm to bovine zonae pellucidae. Proc. Soc. Theriogenology. In Press.
De La Torre, J. F., and G. E. Seidel, Jr. 2003. Timing of dinitrophenol treatment during in vitro culture of bovine embryos. J. Anim. Sci. 81 (Suppl. 1):142 (abstr. #564).
De La Torre-Sanchez, J. F., and G. E. Seidel, Jr. 2003. Effects of glucose concentration and presence of EGF and hormones on bovine oocyte maturation. J. Anim. Sci. 81 (Suppl. 1):186 (abstr. #M32).
Flint, A., Z. Brink, and G. E. Seidel, Jr. 2003. Use of heterospermic insemination and genotyping embryos to compare fertility of flow-sorted sperm from individual bulls. Theriogenology 59:507.
Schenk, J. L., W. B. Henderson, and G. E. Seidel, Jr. 2003. Timed insemination of superovulated heifers with sexed sperm. J. Anim. Sci. 81 (Suppl. 1):118 (abstr. #470).
Seidel, G. E., Jr., Z. Brink, and J. L. Schenk. 2003. Use of heterospermic insemination with fetal sex as the genetic marker to study fertility of sexed sperm. Theriogenology 59:515.
Tubman, L. M., Z. Brink, T. K. Suh, and G. E. Seidel, Jr. 2003. Normality of calves resulting from sexed sperm. Theriogenology 59:517.
Walker, D. J., J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Effects of glucose concentration and presence of EGF and hormones on bovine oocyte maturation. J. Anim. Sci. 81 (Suppl. 1):33 (abstr. #132).
CONNECTICUT
Enright, B.P., X. C. Tian. 2003. Epigenetic Characteristics of Donor Cells for Nuclear Transfer: Levels of Histone Acetylation. Theriogenology 58:251.
Bormann, C. L., B. P. Enright, X. Yang. 2003. Increased parthenogenetic development of bovine oocytes activated with a combinations of phosphorylation and protein synthesis inhibitors. Theriogenology 58:471.
Jeong, B.-S., F. Xue, X. C. Tian. 2003. 5?-aza-2?-deoxycytidine induces expression of PLP1, an X-linked gene normally silent in bovine adult fibroblasts Theriogenology 58:422.
Lee, J.-W., A. Dinnyes, X. Yang. 2003. Development of transgenic embryos following injection of freeze-dried sperm in pigs. Theriogenology 58:305.
Liu, J.-L., L.-Y. Sung, F. Du, M. Julian, S. Jiang, M. Barber, J. Xu, X. C. Tian, X. Yang. 2003. Differential development of rabbit embryos derived from parthenogenesis and nuclear transfer. Theriogenology 58:271.
Nedambale, T. L., S. A. Chaubal, F. Du, W. Groen, D. C. Faber, A. Dinnyes, X. Yang. 2003. Vitrification methods for cryopreservation of in vitro produced cattle embryos. Theriogenology 58: 307.
Wang L., B.-S. Jeong, E. Duan, X. C. Tian. 2003. Effect of donor cell age on number of population doubling of feline fibroblasts cultured in vitro Theriogenology 58:535.
ILLINOIS
Bonde, S., Varzavand, A., Zou, G.-M., Field, E., Walters, E.M., Wheeler, M.B., Zavazava, N. (2003) Porcine-Derived Embryonic Stem Cells are Immune-Privileged and Resistant to Natural Killer Cell Lysis. American Journal of Transplantation (Suppl. 5), S546.
Clark, S.G. Walters, E.M. Beebe, D.J., Wheeler, M.B. (2003). A Novel Integrated In Vitro Maturation and In Vitro Fertilization System In Swine. Theriogenology 59:441.
Clark, S.G., Walters, E.M., Beebe, D. J., M.B. Wheeler. (2003). Determination of the BSA content needed for culture of porcine embryos fertilized in micro
channels. Biol. Reprod. 68 (Suppl. 1) 336.
Donovan ,S.M, Hartke, J.L., Monaco, M.H., Wheeler, M.B. (2003) Over-expression of IGF-I effects on piglet intestinal growth. J. Dairy Sci 86 (Suppl. 1): 134.
Gronlund D.E., Hurley, W.L, Monaco, M.H., Wheeler, M.B., Donovan, S.M. (2003) Over-expression of insulin-like growth factor-I in lactating porcine mammary tissue has a differential effect on amino acid transport systems. J. Dairy Sci 86 (Suppl. 1): 190.
Hartke J.L., Monaco, M.H., Wheeler, M.B., Donovan, S.M. (2003) Effect of a short-term fast on intestinal disaccharidase activity and villus morphology in piglets suckling insulin-like growth factor-I (IGF-I) transgenic sows. J. Dairy Sci 86 (Suppl. 1): 134.
Hartke J.L., Monaco, M.H., Wheeler, M.B., Donovan, S.M. (2003) Intestinal growth and development in piglets suckling insulin like growth factor-I (IGF-I) transgenic sows. J. Dairy Sci.86 (Suppl. 1): 311.
Marshall, K.M., Shanks, R.D., Wheeler, M.B. (2003) Increased nursing intensity stimulated greater lactational response in sows expressing a bovine alpha-lactalbumin transgene. Biol. Reprod. 68 (Suppl. 1) 202.
Monaco, M.H., Hurley, W.L, Wheeler, M.B., Donovan, S.M. (2003) Insulin-like growth factor-I (IGF-I) modulates the process of mammary apoptosis after weaning in IGF-I transgenic pigs. J. Dairy Sci 86 (Suppl. 1): 301.
Walters, E.M., Clark, S.G, Roseman, H., Beebe, D.J., Wheeler, M.B. 2003. Production of Live Piglets Following In Vitro Embryo Culture in Microfluidic Environment. Theriogenology 59:353.
LOUISIANA
Barry, D.M., G. Herholdt, A. Visser, B.B. Hzimhanovich and R.A. Godke. 2003. Development of mammalian embryos and stem cells at micro-gravity conditions at the international space station. Theriogenology 59:337.
Damiani, P., G. Wirtu, F. Miller, C.E. Pope, A. Cole, R.A. Godke and B.L. Dresser. 2003. Development of Giant eland antelope (Taurotragus derbianus) embryos following nuclear transfer with Common eland (Taurotragus oryx) and bovine (Bos taurus) oocytes. Theriogenology 59:390.
Ferguson, C.E., D.J. Kesler, T.R. Davidson, B. Lenard and R.A. Godke. Saving pregnancies in beef cattle after a luteolytic dose of prostaglandin. Theriogenology (In press).
Godke, R.A., Sansinena,M.J., B. Reggio, D. Hylan, A. Klumpp, S.K. Lyle, R.S. Denniston, and D. Paccamonti. 2003. Effect of different pre-maturation treatments on in vitro maturation of equine oocytes and subsequent competence for nuclear transfer. Proc. Havemeyer Workshop. p.13.
Godke,R.A., K. Hebert, A. James, R.S. Denniston, D. Paccamonti. 2003. The potential use of epididymal sperm from bulls and stallions for assisted fertilization. Proc. Havemeyer Workshop. p. 21.
Gómez, M.C., C.E. Pope, A.M. Giraldo, L. Lyons, R.F. Harris, A. King, A. Cole, R.A. Godke and B.L. Dresser. Birth of African Wildcat cloned kittens. Theriogenology (In press).
Klump, A, Aljarrah A, Hylan D, Pinto C, Sansinena M, Eilts B, Godke RA and Paccamonti D. 2003. Follicular aspiration during transition to advance the onset of cyclicity. Proc. Leipzig Expert Workshop on Equine Reproductive Medicine (Abstract).
Klumpp, A. M., R.S. Denniston D. Paccamonti, S.P. Leibo, and R.A. Godke. Embryonic development after holding bovine oocytes in undiluted follicular fluid for a 6-hour prematuration period. Theriogenology (In press).
Landry, A.M., Y. Echelard, M. Murakami, M.G. Collins, R.S. Denniston and R.A. Godke. 2003
Effect of electrofusion method and time of cleavage on bovine embryo development after blastomere fusion at the 2-cell stage. Theriogenology 59:355.
Moisan, A.E., J.W. Lynn, S.P. Leibo, R.S. Denniston and R.A. Godke. Volumetric changes of bovine oocytes resulting from dehydration in disaccharides solution. Theriogenology (In press).
Nel, L., R.A. Godke, B.L. Dresser and P. Damiani. Isolation, culture and cryopreservation of somatic cells obtained from fresh and cooled semen, and fresh milk of gulf coast native sheep. Theriogenology (In press).
Sansinena, M.J. Hylan, D., Klump, A., Reggio, B, Denniston, R.S. and Godke, R.A. 2003. Effect of roscovitine prematuration on in vitro maturation of equine oocytes from small ovarian follicles and subsequent nuclear transfer efficiency. Proc World Equine Vet. Med. Assoc. (Abstract).
Sansinena ,M., P. Taylor, S. Taylor, R.S. Denniston and R.A. Godke. 2003. Production of interspecies nuclear transfer embryos using male and female llama (Lama glama) cell lines. Theriogenology 59:287.
Wirtu, G., P. Damiani, B. Bavister, F. Miller, B. Dresser, R. Godke and E. Pope. 2003. Effects of eland and bovine serum on the development of in vitro-derived bovine embryos. Theriogenology 59:354.
Wirtu, W., C. E. Pope, P. Damiani, B.L. Dresser, R.A. Godke and B.D. Bavister. Insulin improves frequency of cleavage and subsequent development of bovine embryos in vitro. Theriogenology (In press).
WISCONSIN
Fischer-Brown A., D. Northey, J. Parrish, and J. Rutledge 2003. Post-transfer growth of in vitro-produced bovine embryos. Biol Reprod 68 (Supp 1):252.
Fischer-Brown A., R. Monson, D. Northey, J. Rutledge, K. Albrecht, and D. Schaefer 2003. Reduced dietary protein improves pregnancy rates following transfer of in vitro produced bovine embryos. Theriogenology 59(1):364.
d. Miscellaneous publications
CANADA
Martinez, M.F., Adams, G.P., Kastelic, J.P. and Mapletoft, R.J., 2001. Artificial Insemination without Heat Detection in Beef Heifers. Large Animal Veterinary Rounds, 1, 2.
Mapletoft, R.J., Schmutz, S.M. and Searcy, G.P., 2000. Observations on the Inheritance of a Bleeding Disorder in Simmental Cattle. Simmental Country, 21, 18.
Mapletoft, R.J., Barth, A.D., Kastelic, J.P. and Coulter, G.H., 1999. Fertility and Puberty in Young Beef Bulls. Simmental Country, February 1999, 80-81.
LOUISIANA
Ferguson, C.F., O. Perez, K. Hebert , J. Cartmill and R.A. Godke. 2003. Using altrenogest to synchronize estrus in beef heifers. LSU AgCenter Beef Cattle Research Report 32:55-56.
Hebert, K., D. Hylan, R.S. Denniston and R.A Godke. 2003. Administering prostaglandin F2a twice daily for 5 days to nursing beef cows starting 5 days after calving. LSU AgCenter Beef Cattle Research Report 32:58-59.
Godke, R.A. and R.S. Denniston. 2003. Biotechnology and Cattle Production in the Future. OvaGenix Information Day Report 1:47-53.
Godke, R.A., R.S. Denniston and B. Reggio. 2003. Animal biotechnology and the future. Louisiana Agric. pp. 10-14.
IOWA
Youngs, C.R. 2003. Use of gonadotropin releasing hormone for controlled breeding of dairy cattle. Iowa Jersey Today 9(1):9-10.