SAES-422 Multistate Research Activity Accomplishments Report
Sections
Status: Approved
Basic Information
- Project No. and Title: W171 : Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock
- Period Covered: 01/01/2001 to 12/01/2001
- Date of Report: 05/20/2003
- Annual Meeting Dates: 01/11/2002 to 01/11/2002
Participants
Rorie, Rick - Arkansas; Rosenkrans, Charlie - Arkansas; Anderson, Gary - California; Seidel, George - Colorado; Wheeler, Matt - Illinois; Youngs, Curt - Iowa; Godke, Bob - Louisiana; Adams, Gregg - Saskatoon; Mapletoft, Reuben - Saskatoon.
The meeting was called to order at 7:00 p.m. by Chairman Charlie Rosenkrans. Members were welcomed, and Charlie expressed regrets to the group from Jerry Yang (Connecticut), Tom Bunch (Utah), and Fred Menino (Oregon) who were unable to attend. Charlie Rosenkrans appointed Curt Youngs as acting secretary due to Jerry Yang‘s absence at the meeting. Jerry will resume his duties as secretary next year. Bob Godke moved and Rick Rorie seconded to have Curt Youngs assume the duties of president next year. Motion passed unanimously.
Bob Godke gave a brief overview of the history and purposes of the W-171 USDA Regional Research Project for the benefit of our new members. Neither Kelvin Koong (administrative advisor) nor Mark Mirando (CSREES) was able to attend, so the group did not receive comments from the administrative advisor or CSREES representative. Charlie Rosenkrans volunteered to contact both Dr. Koong and Dr. Mirando to request that any pertinent information be shared with the W-171 members via either e-mail or snail mail.
Bob Godke moved and Rick Rorie seconded to remove from the table the motion to add the Nevada station to the W-171 group. Motion passed. After prolonged discussion, a consensus was reached that insufficient information was available on which to base a decision concerning the "fit" of the proposed Nevada members with the objectives of the W-171 project. It was decided that Charlie Rosenkrans should contact the Nevada station to request that they attend the next W-171 meeting in order to answer questions for the group.
Options for the 2003 W-171 meeting were discussed. If the next meeting is held near the time of the International Embryo Transfer Society (IETS) annual meeting, it was agreed that it should be held before IETS in order to minimize conflicts with spring semester teaching schedules. Three options were proposed: Los Angeles (Ed Fonda -host?), San Francisco (Gary Anderson - host?), or Denver (George Seidel - host?). Bob Godke will contact Ed Fonda to see if he would be willing to host the group if we meet in Los Angeles. If the meeting were to be held at IETS in Auckland, New Zealand, Robin Tervit of the local organizing committee is the contact person. Members not in attendance at this year‘s meeting should be contacted about their input, and a decision should be made by September 1, 2002. Curt Youngs shall be in charge of final arrangements.
Charlie Rosenkrans reminded everyone to send to him, in electronic form, their accomplishments under each objective and their publication list no later than February 15, 2002. Please be sure to include graduate student theses and dissertations in the publication list.
No new applications for membership were submitted to the W-171 group during the past year, so no action on membership was necessary. Discussion was held concerning persons that potentially could be recruited to become W-171 members. Two suggestions were put forth: Pete Hansen, University of Florida (to be contacted by Curt Youngs), and Allan King, University of Guelph (to be contacted by Rick Rorie).
Discussions were held concerning the joint research projects. The first project centered on the efficiency of the Heatwatch system for detection of estrus. Four stations (Louisiana, Arkansas, Utah, and Iowa) indicated they had used the system with cattle. One station (Saskatchewan) used the system in wapiti, one station in sheep (Arkansas), and one station with goats (Louisiana). The system doesn‘t work well in goats, as there are too many false mounts (ovulations or lack thereof verified via laparotomy). A discussion was held concerning the efficiency of the system in dairy cattle versus beef cattle, as well as the influence of body condition score. An idea was proposed to enhance multi-species use by mounting a transmitter on a male and a receiver on a female. Bob Godke will send Gregg Adams a draft protocol for a group project. John Riesen also expressed interest in the protocol.
The second project discussion centered on the IFAS grant proposal that had been submitted to examine the effect of oocytes age and semen quality on the efficiency of in vitro fertilization. It was suggested that costs should be updated: 70 cents/oocyte and $28/straw of semen. It was agreed that we should try to gather preliminary data in the upcoming year. Charlie Rosenkrans agreed to e-mail the experimental design to all stations.
No substantial progress had been made on markers of oocytes quality. The microchannel device is still viewed as highly desirable, but due to lack of availability this project will need to be deferred. Once available, though, the device could be used across stations to compare factors such as time of maturation. Reuben Mapletoft asked if Matt Wheeler would be willing to organize a pre-conference (IETS) or post-conference symposium on oocyte quality. The W-171 group could then refine the experimental design for projects in the coming year. It was agreed that a specific action plan should be developed. The plan should name the person responsible and a deadline should be indicated.
Individual station reports were then given for the stations in attendance. Interesting discussions ensued.
Reuben Mapletoft moved and Bob Godke seconded to adjourn the meeting. The motion carried, and the meeting was adjourned at 9:40 p.m.
Respectfully submitted,
Curt Youngs
Acting Secretary
Bob Godke gave a brief overview of the history and purposes of the W-171 USDA Regional Research Project for the benefit of our new members. Neither Kelvin Koong (administrative advisor) nor Mark Mirando (CSREES) was able to attend, so the group did not receive comments from the administrative advisor or CSREES representative. Charlie Rosenkrans volunteered to contact both Dr. Koong and Dr. Mirando to request that any pertinent information be shared with the W-171 members via either e-mail or snail mail.
Bob Godke moved and Rick Rorie seconded to remove from the table the motion to add the Nevada station to the W-171 group. Motion passed. After prolonged discussion, a consensus was reached that insufficient information was available on which to base a decision concerning the "fit" of the proposed Nevada members with the objectives of the W-171 project. It was decided that Charlie Rosenkrans should contact the Nevada station to request that they attend the next W-171 meeting in order to answer questions for the group.
Options for the 2003 W-171 meeting were discussed. If the next meeting is held near the time of the International Embryo Transfer Society (IETS) annual meeting, it was agreed that it should be held before IETS in order to minimize conflicts with spring semester teaching schedules. Three options were proposed: Los Angeles (Ed Fonda -host?), San Francisco (Gary Anderson - host?), or Denver (George Seidel - host?). Bob Godke will contact Ed Fonda to see if he would be willing to host the group if we meet in Los Angeles. If the meeting were to be held at IETS in Auckland, New Zealand, Robin Tervit of the local organizing committee is the contact person. Members not in attendance at this year‘s meeting should be contacted about their input, and a decision should be made by September 1, 2002. Curt Youngs shall be in charge of final arrangements.
Charlie Rosenkrans reminded everyone to send to him, in electronic form, their accomplishments under each objective and their publication list no later than February 15, 2002. Please be sure to include graduate student theses and dissertations in the publication list.
No new applications for membership were submitted to the W-171 group during the past year, so no action on membership was necessary. Discussion was held concerning persons that potentially could be recruited to become W-171 members. Two suggestions were put forth: Pete Hansen, University of Florida (to be contacted by Curt Youngs), and Allan King, University of Guelph (to be contacted by Rick Rorie).
Discussions were held concerning the joint research projects. The first project centered on the efficiency of the Heatwatch system for detection of estrus. Four stations (Louisiana, Arkansas, Utah, and Iowa) indicated they had used the system with cattle. One station (Saskatchewan) used the system in wapiti, one station in sheep (Arkansas), and one station with goats (Louisiana). The system doesn‘t work well in goats, as there are too many false mounts (ovulations or lack thereof verified via laparotomy). A discussion was held concerning the efficiency of the system in dairy cattle versus beef cattle, as well as the influence of body condition score. An idea was proposed to enhance multi-species use by mounting a transmitter on a male and a receiver on a female. Bob Godke will send Gregg Adams a draft protocol for a group project. John Riesen also expressed interest in the protocol.
The second project discussion centered on the IFAS grant proposal that had been submitted to examine the effect of oocytes age and semen quality on the efficiency of in vitro fertilization. It was suggested that costs should be updated: 70 cents/oocyte and $28/straw of semen. It was agreed that we should try to gather preliminary data in the upcoming year. Charlie Rosenkrans agreed to e-mail the experimental design to all stations.
No substantial progress had been made on markers of oocytes quality. The microchannel device is still viewed as highly desirable, but due to lack of availability this project will need to be deferred. Once available, though, the device could be used across stations to compare factors such as time of maturation. Reuben Mapletoft asked if Matt Wheeler would be willing to organize a pre-conference (IETS) or post-conference symposium on oocyte quality. The W-171 group could then refine the experimental design for projects in the coming year. It was agreed that a specific action plan should be developed. The plan should name the person responsible and a deadline should be indicated.
Individual station reports were then given for the stations in attendance. Interesting discussions ensued.
Reuben Mapletoft moved and Bob Godke seconded to adjourn the meeting. The motion carried, and the meeting was adjourned at 9:40 p.m.
Respectfully submitted,
Curt Youngs
Acting Secretary
Accomplishments
Objective 1. Understanding the developmental biology and underlying mechanisms of fertilization and embryonic development.
A. Managing the Female
LA studied benefits of having a vasectomized bull or androgenic cow to aid in getting females to cycle and detecting estrus. There was no advantage of these animals in AI pregnancy rates, possibly because cattle were well managed. AR studied 3 commercially available methods of electronic monitoring of estrous behavior in cattle; all three were effective, and there were differences in cost and convenience. Sask studied timing of AI, 23-33 h after E2 injection and CIDR removal. No effects were noted on pregnancy rates or sex ratios of calves over this time interval. IA developed software to assist producers in choosing from a plethora of estrus synchronization procedures for cattle with inputs of costs, expected success rates, etc.
B. Studies Focusing on Sperm
CA studied the DRKD protein on porcine sperm, which first appears in the epididymis. Several isoforms were found, and amounts varied seasonally. LA studied deterioration of stallion epididymal sperm when castrated testes-epididymides were stored at 50C post-castration for up to 4 days. Sperm remained reasonably viable for 3 days. WA studied cooling and storing stallion sperm at 50C; it was found that egg yolk-based extenders resulted in much less chromatin damage than milk-based extenders. CA studied evaluation of bull and stallion sperm via flow cytometry, concentrating on the JC-1 stain. Interpretation of data using this stain remains problematic. IA studied use of centrifugation of sperm in Bovipure to sex bovine sperm; no effect on sex ratio was found, but cleavage rates improved slightly. LA injected sperm directly into follicles of mares, but results were not promising.
C. The Oocyte, Embryo, Fetus, and Offspring
LA studied potential benefits of flushing post-partum beef cows with high energy feed and/or injection of bovine growth hormone for transvaginal recovery of oocytes; all cows also received an FSH injection. Oocyte recovery and blastocyst development rates after IVF were lower with the flushing treatment than no treatment; growth hormone was beneficial alone but not with energy supplementation. A similar experiment was done with mares and equine growth hormone, and there was no benefit from growth hormone. Recovered equine oocytes were fertilized via sperm injection, with some pregnancies and a foal resulting after embryo transfer. Sask showed that echo-texture of bovine follicles is correlated with oocyte quality and subsequent blastocyst production. CO showed that roscovitine could reversibly arrest nuclear maturation of equine oocytes. Embryo quality was improved by transient arrest followed by sperm injection. WI attempted to slow bovine maturation rates by culture at 350C. Blastocyst production declined at lower temperatures and longer maturation times. WI also showed that maturation of bovine oocytes with LH was superior to FSH in terms of embryo production. IL developed microfluidic devices to mature bovine oocytes and remove cumulus cells, and they showed that development of mouse embryos was enhanced by these procedures. OR characterized metalloproteinases and their inhibitors in pregnant and non-pregnant ovine uteri and day 9-15 embryos. All genes studied were transcribed in pregnant and non-pregnant uteri, but some were missing in embryos. OR also used day-7 bovine embryos to study endodermal cell migration on various extracellular matrix proteins in vitro. CO studied calves resulting from IVF with sexed and control sperm; embryos were cultured with and without insulin. There was no effect of sexing, but insulin may have led to more problems with neonatal calves.
Objective 2. Development of genetically modified animals to enhanced desirable traits.
A.. Cloning by Nuclear Transplantation
Utah studied potential benefits of activating oocytes with mitogens. This approach turned out not to be promising. IL studied various methods of activating porcine oocytes parthenogenetically. Some approaches were quite successful. CT studied additional permutations and combinations of approaches to activation of bovine oocytes. Chemical plus electrical combinations resulted in up to 40% parthenogenetic blastocysts. UT studied Ca++ release due to the arginine-glycine-aspartic acid tripeptide. This approach turned out to release IP3 sensitive Ca++ stores. CT studied a novel approach to removal of chromosomes from oocytes, hypertonic sucrose. This greatly facilitated the process, as chromosomes migrated to surface blebs. LA showed that bovine cumulus cells were superior nuclear donors to fibroblasts. They also studied using bovine cytoplasts to process nuclei from equine fibroblasts, with reasonable rates of early development of embryos; they surmised that this approach could be used for screening procedures. TX studied microarrays of gene expression of cloned vs IVF embryos. Most genes were expressed similarly, but a number of genes from the donor cells continued to be expressed inappropriately in the first few cleavage divisions. Utah studied telomerase expression, which was not different in IVF vs cloned bovine embryos. CO studied d-75 cloned pregnancies. There was more IGF-2 mRNA in livers of clones, but less IGF-1 and -2 in cotyledons of cloned placentae; umbilical cords were thicker in clones. No differences could be found in amounts of mRNA for angiogenic factors between clones and controls. LA found that there was less variation in blood hormone levels within bovine clonal sets than within other animals, but that variation in growth rates was not reduced.
B. Transgenesis and Stem Cells
CA studied porcine embryonic germ cell incorporation into chimeras by comparing blastocoele injection vs addition to the perivitelline space pre-compaction. Both procedures resulted in success at low rates. UT showed that the lytic peptide shiva-1 could be expressed in milk of transgenic mice at moderate rates using a bovine casein promoter. LA made 8 transgenic kids by cloning from a line of cells derived from a transgenic fetus sired by a transgenic buck. IL made 2 lines of transgenic pigs, one for bovine alpha lactalbumin and one for IGF-1. Transgenic mothers resulted in faster growth of suckling piglets when transgenic for alpha lactalbumin but not IGF-1. Piglets suckling sows transgenic for IGF-1 had higher jejunal villi than controls.
Future Plans
AR will investigate the effect of bovine somatotropin on embryo quality, embryo transfer pregnancy rates, and follicular wave dynamics. CA will characterize sperm and oocyte molecules involved with fertilization and utility of embryonic germ cells for transgenesis. CO will study bovine and equine in vitro oocyte maturation and culture, as well as gene expression in placentae of cloned versus control pregnancies. CT will investigate methods for oocyte activation and enucleation, as well as methods for oocyte cryopreservation. IL will continue research with transgenic swine and embryo culture in microfluidic devices. IA will compare the efficacy of dinoprost versus cloprostenol for induction of estrus in postpartum dairy cows. LA will investigate various in vitro embryo culture systems and will continue research with nuclear transfer and transgenesis in farm animals. OR will study transcription factor expression and cellular differentiation during bovine embryonic development. TX will continue studies with differential gene expresssion in cloned versus control embryos. UT will evaluate protein supplment and gorwth factors for use in in vitro embryo culture. WA will examine the relationship between sperm DNA quality and fertility rates. WI will investigate methods for bovine oocyte cryopreservation.
A. Managing the Female
LA studied benefits of having a vasectomized bull or androgenic cow to aid in getting females to cycle and detecting estrus. There was no advantage of these animals in AI pregnancy rates, possibly because cattle were well managed. AR studied 3 commercially available methods of electronic monitoring of estrous behavior in cattle; all three were effective, and there were differences in cost and convenience. Sask studied timing of AI, 23-33 h after E2 injection and CIDR removal. No effects were noted on pregnancy rates or sex ratios of calves over this time interval. IA developed software to assist producers in choosing from a plethora of estrus synchronization procedures for cattle with inputs of costs, expected success rates, etc.
B. Studies Focusing on Sperm
CA studied the DRKD protein on porcine sperm, which first appears in the epididymis. Several isoforms were found, and amounts varied seasonally. LA studied deterioration of stallion epididymal sperm when castrated testes-epididymides were stored at 50C post-castration for up to 4 days. Sperm remained reasonably viable for 3 days. WA studied cooling and storing stallion sperm at 50C; it was found that egg yolk-based extenders resulted in much less chromatin damage than milk-based extenders. CA studied evaluation of bull and stallion sperm via flow cytometry, concentrating on the JC-1 stain. Interpretation of data using this stain remains problematic. IA studied use of centrifugation of sperm in Bovipure to sex bovine sperm; no effect on sex ratio was found, but cleavage rates improved slightly. LA injected sperm directly into follicles of mares, but results were not promising.
C. The Oocyte, Embryo, Fetus, and Offspring
LA studied potential benefits of flushing post-partum beef cows with high energy feed and/or injection of bovine growth hormone for transvaginal recovery of oocytes; all cows also received an FSH injection. Oocyte recovery and blastocyst development rates after IVF were lower with the flushing treatment than no treatment; growth hormone was beneficial alone but not with energy supplementation. A similar experiment was done with mares and equine growth hormone, and there was no benefit from growth hormone. Recovered equine oocytes were fertilized via sperm injection, with some pregnancies and a foal resulting after embryo transfer. Sask showed that echo-texture of bovine follicles is correlated with oocyte quality and subsequent blastocyst production. CO showed that roscovitine could reversibly arrest nuclear maturation of equine oocytes. Embryo quality was improved by transient arrest followed by sperm injection. WI attempted to slow bovine maturation rates by culture at 350C. Blastocyst production declined at lower temperatures and longer maturation times. WI also showed that maturation of bovine oocytes with LH was superior to FSH in terms of embryo production. IL developed microfluidic devices to mature bovine oocytes and remove cumulus cells, and they showed that development of mouse embryos was enhanced by these procedures. OR characterized metalloproteinases and their inhibitors in pregnant and non-pregnant ovine uteri and day 9-15 embryos. All genes studied were transcribed in pregnant and non-pregnant uteri, but some were missing in embryos. OR also used day-7 bovine embryos to study endodermal cell migration on various extracellular matrix proteins in vitro. CO studied calves resulting from IVF with sexed and control sperm; embryos were cultured with and without insulin. There was no effect of sexing, but insulin may have led to more problems with neonatal calves.
Objective 2. Development of genetically modified animals to enhanced desirable traits.
A.. Cloning by Nuclear Transplantation
Utah studied potential benefits of activating oocytes with mitogens. This approach turned out not to be promising. IL studied various methods of activating porcine oocytes parthenogenetically. Some approaches were quite successful. CT studied additional permutations and combinations of approaches to activation of bovine oocytes. Chemical plus electrical combinations resulted in up to 40% parthenogenetic blastocysts. UT studied Ca++ release due to the arginine-glycine-aspartic acid tripeptide. This approach turned out to release IP3 sensitive Ca++ stores. CT studied a novel approach to removal of chromosomes from oocytes, hypertonic sucrose. This greatly facilitated the process, as chromosomes migrated to surface blebs. LA showed that bovine cumulus cells were superior nuclear donors to fibroblasts. They also studied using bovine cytoplasts to process nuclei from equine fibroblasts, with reasonable rates of early development of embryos; they surmised that this approach could be used for screening procedures. TX studied microarrays of gene expression of cloned vs IVF embryos. Most genes were expressed similarly, but a number of genes from the donor cells continued to be expressed inappropriately in the first few cleavage divisions. Utah studied telomerase expression, which was not different in IVF vs cloned bovine embryos. CO studied d-75 cloned pregnancies. There was more IGF-2 mRNA in livers of clones, but less IGF-1 and -2 in cotyledons of cloned placentae; umbilical cords were thicker in clones. No differences could be found in amounts of mRNA for angiogenic factors between clones and controls. LA found that there was less variation in blood hormone levels within bovine clonal sets than within other animals, but that variation in growth rates was not reduced.
B. Transgenesis and Stem Cells
CA studied porcine embryonic germ cell incorporation into chimeras by comparing blastocoele injection vs addition to the perivitelline space pre-compaction. Both procedures resulted in success at low rates. UT showed that the lytic peptide shiva-1 could be expressed in milk of transgenic mice at moderate rates using a bovine casein promoter. LA made 8 transgenic kids by cloning from a line of cells derived from a transgenic fetus sired by a transgenic buck. IL made 2 lines of transgenic pigs, one for bovine alpha lactalbumin and one for IGF-1. Transgenic mothers resulted in faster growth of suckling piglets when transgenic for alpha lactalbumin but not IGF-1. Piglets suckling sows transgenic for IGF-1 had higher jejunal villi than controls.
Future Plans
AR will investigate the effect of bovine somatotropin on embryo quality, embryo transfer pregnancy rates, and follicular wave dynamics. CA will characterize sperm and oocyte molecules involved with fertilization and utility of embryonic germ cells for transgenesis. CO will study bovine and equine in vitro oocyte maturation and culture, as well as gene expression in placentae of cloned versus control pregnancies. CT will investigate methods for oocyte activation and enucleation, as well as methods for oocyte cryopreservation. IL will continue research with transgenic swine and embryo culture in microfluidic devices. IA will compare the efficacy of dinoprost versus cloprostenol for induction of estrus in postpartum dairy cows. LA will investigate various in vitro embryo culture systems and will continue research with nuclear transfer and transgenesis in farm animals. OR will study transcription factor expression and cellular differentiation during bovine embryonic development. TX will continue studies with differential gene expresssion in cloned versus control embryos. UT will evaluate protein supplment and gorwth factors for use in in vitro embryo culture. WA will examine the relationship between sperm DNA quality and fertility rates. WI will investigate methods for bovine oocyte cryopreservation.
Impacts
- Methods have been developed that enable the harvest of oocytes from cattle, horses, goats, and pigs using transvaginal ultrasound-guided procedures. This development allows oocytes to be harvested from cyclic, postpartum, and early pregnant females for subsequent embryo production using standard in vitro fertilization protocols, increasing the efficiency of animal production per female.
- Methods for enhanced in vitro oocyte maturation, in vitro fertilization, and in vitro culture of embryos have been developed. This breakthrough enables embryos to be produced in vitro but transferred to recipient females using standard non-surgical embryo transfer techniques, leading to increased improvement in livestock production.
- Improved methods for oocyte activation and enucleation have been developed. These enhancements to existing procedures will significantly aid in the more efficient production of nuclear transfer offspring.
- Offspring produced via in vitro fertilization with sexed versus unsexed bovine sperm were compared and were found to be normal and not different from one another.
- An innovative microchannel culture device shows great promise for individual culture of oocytes and embryos. This development will enable researchers to more easily identify genes of importance to embryonic development that would be more difficult to perform with culture of groups of oocytes or embryos.
Publications
REFEREED JOURNAL ARTICLES:
ARKANSAS
Brown, A H, D W Kellogg, Z B Johnson, R W Rorie, W K Coblentz, B A Sandelin, K E Lesmeister and W R Jackson. 2001. Growth, luteal activity and pregnancy rates of three breed types of dairy heifers in a forage based development program. Prof. Anim. Sci. 17:20-26
Burke, J.M., R.W. Rorie and G.A. Perry. 2002. Changes in ovarian function in mature beef cows grazing endophyte-infected tall fescue. Theriogenology (in press).
Burke, J M, R W Rorie, E L Piper and W G Jackson. 2001. Reproductive responses to grazing endophyte-infected tall fescue in postpartum beef cows. Theriogenology 56:357-369.
Kreider, D L, R Rorie, D Brown, F Miller and S Wright. 2001. Enhancement of ovulation rate and litter size in gilts. J. Anim. Sci. 79:1691-1696.
Melnychuk, V.L., M.A. Cooper, R.W. Rorie, J.D. Kirby and N.B. Anthony. 2001. Characterization of ovary status using ultrasound techniques. J. Poultry Sci. (in press)
Rorie, R.W., T.R. Bilby, and T.D. Lester. 2002. Application of electronic estrus detection technology to reproductive management of cattle. Theriogenology (in press).
CALIFORNIA
Berger, T. and Dally, M. 2001. Do sire-dam interactions contribute significantly to fertility comparisons in heterospermic insemination trials? Theriogenology 56:535-543.
Bertolini, M., J.B. Mason, S.E. Beam, G.F. Carneiro, M.L. Sween, D.J. Kominek, A.L. Moyer, T.R. Famula, R.D. Sainz and G.B. Anderson. 2002. Morphology and morphometry of in vitro-and in vitro-produced concepti from early pregnancy to term and association with high birth weights. Theriogenology (in press).
Carneiro, G., P. Lorenzo, C. Pimental, L. Pagoraro, M. Bertolini, B. Ball, G. Anderson and I. Liu. 2001. The influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol and fetal calf serum and in vitro maturation and parthenogenic development in equine oocytes. Biology of Reproduction 65:899-905.
Gravance, C.G., Garner, D.L., Miller, M.G. and Berger, T. 2001. Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function. Reprod. Tox. 15:5-10.
Jelks, K., Berger, T., Horner, C., and Miller, M.G. 2001. Chlorohydrin induced changes in sperm fertilizing ability in the rat: association with diminished sperm ATP levels and motility. Reprod. Tox. 15:11-20.
COLORADO
Choi, Y.H., E.M. Carnevale, G.E. Seidel, Jr. and E.L. Squires. 2001. Effects of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology 56:661-670.
Choi, Y.H., Y.G. Chung, G.E. Seidel, Jr. and E.L. Squires. 2001. Developmental capacity of equine oocytes matured and cultured in equine trophoblast- conditioned media. Theriogenology 56:329-339.
Landim-Alvarenga, F.C., M.A. Alvarenga, G.E. Seidel, Jr., E.L. Squires and J.K. Graham. 2001. Penetration of zona-free hamster, bovine and equine oocytes by stallion and bull spermatozoa pretreated with equine follicular fluid, dilauroylphosphatidylcholine or calcium ionophore A23187. Theriogenology 56:937-953.
Lane, M., M.K. O‘Donovan, E.L. Squires, G.E. Seidel, Jr. and D.K. Gardner. 2001. Assessment of metabolism of equine morula and blastocysts. Molec. Reprod. Develop. 59:33-37.
Oberstein, N., M.K. O‘Donovan, J.E. Bruemmer, G.E. Seidel, Jr., E.M. Carnevale and E.L. Squires. 2001. Cryopreservation of equine embryos by open pulled straw, cryoloop, or conventional slow cooling methods. Theriogenology 55:607-613.
Seidel, G.E., Jr. 2001. Cloning, transgenesis, and genetic variance in animals. Cloning and Stem Cells 4:251-256.
Coutinho da Silva, M.A., E.M. Carnevale, L.J. Maclellan, G.E. Seidel, Jr. and E.L. Squires. 2002. Effect of time of oocyte collection and site of insemination on oocyte transfer in mares. J. Anim. Sci. (in press).
Li, G.-P., G.E. Seidel, Jr. and E.L. Squires. 2002. Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa. Theriogenology (in press).
Li, G.-P., G.E. Seidel, Jr. and E.L. Squires. 2002. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology (in press).
CONNECTICUT
Deng, M.Q. and X. Yang. 2001. Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection. Molec. Reprod. Devel. 58:1-6.
Dinnyes, A., L. Liu, Y. Dai, M. Barber, J. Xu, P. Zhou, and X. Yang. 2001. Development of cloned embryos from adult rabbit fibroblast cells. Biol. Reprod. 64:257-263.
Jeong, B.S. and X. Yang. 2001. Cysteine, glutathione, and percoll treatments improve porcine oocyte maturation and fertilization in vitro. Molec. Reprod. Devel. 59:330-335.
Tian, XC, C. Kubota, and X. Yang. 2001. Cloning of aged animals - a medical model for tissue and organ regeneration. Trends in Cardiovascular Medicine 11:313-317.
Xu, J. and X. Yang. 2001. Telomerase activity in early bovine embryos derived from pathenogenetic activation and nuclear transfer. Biol. Reprod. 64:770-774.
Yang, X. and J. Xu. 2001. Developmental changes of telomerase in oocytes and embryos. Advances in Reproduction 6:63-68.
Enright, B., M. Taneja, J. Riesen, X.C. Tian, J. Fortune, and X. Yang. 2002. Normal onset of puberty in cloned heifers of an aged Holstein cow. Biol. Reprod (in press).
Govoni, K.E., X.C. Tian, G.M. Kazmer, M. Taneja, B.P. Enright, A.L. Rivard, X. Yang, and S.A. Zinn. 2002. Age-related changes of the somatotropic axis in cloned Holstein calves. Biol. Reprod. (in press).
Liu, J.L., L.-Y. Sung, M. Barber, and X. Yang. 2002. Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes. Biol. Reprod. (in press).
Tian, X.C., P. Lonergan, A.E.O. Evans, and X. Yang. 2002. Dynamics of MPF and MAPK in oocytes matured in vitro for 20 vs. 40 hours. Molec. Reprod. Devel. (in press).
ILLINOIS
Boston, W.S., G.T. Bleck, M.B. Wheeler, D.J. Miller. 2001. The effects of increased expression of alpha-lactalbumin in transgenic mice on milk yield and pup growth. J. Dairy Sci. 84:620-622.
Donovan, S. M. , M. H. Monaco, G. T. Bleck, J. B. Cook, M. S. Noble, W.L. Hurley and M.B. Wheeler. 2001. Transgenic over-expression of bovine alpha-lactalbumin and human insulin-like growth factor-I in porcine mammary gland. J. Dairy Sci. 84 (E. Suppl.): E216-E222. (http://www.adsa.org/jds/papers/2001/jds_es216.pdf)
Glasgow, I.K., Zeringue, H.C., Beebe, D.J., Choi, S.-J., Lyman, J., Chan, N.G., Wheeler, M.B. 2001. Handling individual mammalian embryos using microfluidics. IEEE Transactions on Biomedical Engineer 48:570-577.
Wheeler, M.B. And Walters, E.M., 2001. Transgenic technology and applications in swine. Theriogenology 56:1345-1370.
Zeringue, H., M. B. Wheeler and D. J. Beebe, 2001. Removal of cumulus from mammalian oocytes using microfluidic techniques. Biomedical Microdevices 3:219-224.
IOWA
Youngs, C.R. 2001. Factors influencing the success of embryo transfer in the pig. Theriogenology 56:1311-1320.
LOUISIANA
Han, Y., M. Meintjes, K. Graff, R. Denniston, L. Zhang, C. Ziomek and R.A. Godke. 2001. Production of fresh and frozen-thawed transplant offspring from latter stage IVF-derived caprine embryos. Vet. Rec. 149:714-716.
Meintjes, M., B. Eilts, K. Graff, R. Denniston, D. Paccamonti and R. Godke. 2001. Transvaginal intrafollicular sperm cell injection in the cyclic mare. Internat‘l. Symp. Equine Embryo Transf. Monograph 3:39-40.
Murakami, M., A. Boediono, O. Perez, E. Ferguson, R. S. Denniston, E. Behboodi and R. A. Godke. 2001. The use of adult bovine skin fibroblasts cultured at different intervals prior to nuclear transfer. Arch. Anim. Breeding 44:175-177.
Poleo, G.A., R. S. Denniston, B. Reggio, R. A. Godke and T. R. Tiersch. 2001. Fertilization of eggs of Zebrafish (Danio rerio) by intracytoplasmic sperm injection. Biol. Reprod. 65:321-326.
Reggio, B.C., A.N. James, H. L. Green, W. G. Gavin, E. Behboodi, Y. Echelard and R. A. Godke. 2001. Cloned transgenic offsring in goats resulting from somatic cell nuclear transfer in goats: Oocytes derived from FSH-stimulated and nonstimulated ovaries. Biol Reprod. 65:1528-1533.
Reggio, B.C., M. Sansinena, R.A. Cochran, A. Guitreau, J.A. Carter, R.S. Denniston and R.A. Godke. 2001. Nuclear transfer embryos in the mare. Internat‘l. Symp. Equine Embryo Transf. Monograph 3:45-46.
Boediono, A., T. Suzuki and R.A. Godke. The development of in vitro-derived hybrid and straightbred beef cattle embryos during in vitro culture. Anim. Reprod. Sci. (in press).
Carter, J.A., S. Bellow, M. meintjes, O. Perez, E. Ferguson and R. A. Godke. Transvaginal ultrasound-guided oocyte aspiration for production of embryos in vitro. Arch. Anim. Breeding (in press).
Cochran, R., M. Meintjes, D. Hylan, M. Sansinena, R. S. Denniston D. Paccamonti and R.A. Godke. Follicle growth in mares for oocyte production. In: From Epididymis to Embryo. Havemeyer Foundation. (in press)
Han, Y., Y. Han, R.S. Denniston, A. Boediono, C. Ziomek and Godke. Development of IVF-derived caprine embryos with four different somatic cell co-cultures. Small Ruminant Res. (in press).
Murakami, M., S. Ideguchi, M. Fahrudin and R.A. Godke. Bovine nuclear transplant using fluorescent embryos after microinjection with the EGFP gene. Anim. Reprod. Develop. (in press).
Pinto, C.R.F., D.L. Paccamonti, B.E. Eilts, C.R. Short and R.A. Godke. Effect of nitric oxide synthetase inhibitors on ovulation in hCG-stimulated mares. Theriogenology (in press).
Sansinena, M., Paccamonti, D., C. Cochran, M. Meintjes, B. Reggio, R. S. Denniston, C. Pinto, B. Eilts and R.A.Godke. Developing the intracytoplasmic sperm injection (ICSI) procedure for the horse. In: From Epididymis to Embryo. Havemeyer Foundation. (in press).
Songasen, N., I. Yu, S. Murton, D. Paccamonti, B.E.Eilts, R. A. Godke and S.P. Leibo. Osmotic sensitivity of Canine Spermatozoa. Cryobiology (in press).
Yu, I.J., N. Songsasen, R.A. Godke and S.P. Leibo. Differences among male dogs in response of their spermatozoa cryopreservced at various coolling and warnimg rates. Cryobiology (In press).
OREGON
Al-Aghbari, A.M. and A.R. Menino, Jr. 2002. Survival of oocytes recovered from vitrified sheep ovarian tissues. Anim. Reprod. Sci.(in press)
Schilperoort-Haun, K.R. and A.R. Menino, Jr. 2002. Evaluation of extracellular matrix proteins and tissue inhibitor of matrix metalloproteinases-2 on bovine inner cell mass outgrowth in vitro. In Vitro Cell. Dev. Biol.-Animal (in press).
UTAH
Kitiyanant, Y., J. Saikhun, B. Chrisalee, K.L. White and K. Pavasuthipaisit. 2001. Somatic Cell Cloning in Buffalo (Bubalus bubalis): Effects of interspecies cytoplasmic recipients and activation procedures. Cloning and Stem Cells 3:97-104.
Thonabulsombat, C., W.A. Reed, J.D. Morrey, K. Bates, R. Smart and K.L. White. 2001. Transgenic mice carrying a gene for green fluorescent protein fused to a lytic peptide, Shiva 1, under control of the bovine 8-Casein regulatory region. Transgenics 3:183-197.
Viets, L.N., K.D. Campbell and K.L. White. 2001. Pathways involved in RGD-mediated calcium transients in mature bovine oocytes. Cloning and Stem Cells 3:105-113.
Wang, S., W.C. Foote, D.L. Sutton, A. Maciulis, J.M. Miller, R.C. Evans, G.R. Holyoak, J.W. Call, T.D. Bunch, W.D. Taylor and M.R. Marshall. 2001. Preventing experimental vertical transmission of scrapie by embryo transfer. Theriogenology. 56:315-327.
Wang, S., K.E. Panter, R.C. Evans, and T.D. Bunch. 2001. The effects of pokeweed mitogen (PWM) and phytohemagglutinin (PHA) on bovine oocyte maturation and embryo development in vitro. Anim. Reprod. Sci. 67:215-220.
WASHINGTON
Bosch, P., J.M. deAvila, J.E. Ellington, and R.W. Wright, Jr. 2001. Heparin and Ca2+-free medium can enhance release of bull sperm attached to oviductal epithelial cell monolayers. Theriogenology 56:247-260.
WISCONSIN
Rutledge, J.J. 2001. Greek temples, tropical kine and recombination load. Livestock Production Science 68:171-179.
Rutledge, J.J. 2001. Use of embryo transfer and IVF to bypass effects of heat stress. Theriogenology 55:105-111.
Al-Katanani, Y.M., M. Drost, R.L. Monson, J.J. Rutledge, C.E. Krininger III, C.J. Block, W.W. Thatcher, and P.J. Hansen. 2002. Pregnancy rates following timed embryo transfer with fresh or vitrified in vitro produced embryos in lactating dairy cows under heat stress conditions. Theriogenology (in press).
Men, H., R.L. Monson, and J.J. Rutledge. 2002. Effect of maturation process and maturation protocols on bovine oocyte freezing tolerance. Theriogenology (in press).
BOOKS, CHAPTERS IN BOOKS, PROCEEDINGS, AND THESES:
CALIFORNIA
Berger, T. and Dally, M. 2001. Is there a sire-dam interaction in sperm fertilizing potential? California Agriculture 55:25.
COLORADO
Choi, Y.H., G.E. Seidel, Jr., S. Boyazoglu and E.L. Squires. 2001. Polyvinylalcohol is superior to bovine serum albumin in equine IVF medium. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 28-29.
Garner, D., J. Schenk and G. Seidel, Jr. 2001. Chromatin stability in sex-sorted sperm. Proc. VII Int. Congr. Androl., pp. 3-7.
Landim-Alvarenga, F.C., S.E.A. Boyazoglu, G.E. Seidel, Jr. and E.L. Squires. 2001. Effect of fetuin on zona hardening and the distribution of cortical granules in equine oocytes matured in vitro. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 26-27.
Oberstein, N., M.K. O‘Donovan, J.E. Bruemmer, M. Lane, G.E. Seidel, Jr., E.M. Carnevale and E.L. Squires. 2001. Cryopreservation of equine embryos by open pulled straw, cryoloop or conventional slow cooling methods. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 54-56.
O‘Donovan, M.K., N. Oberstein, J.E. Bruemmer, M. Lane, G.E. Seidel, Jr., D.K. Gardner and E.L. Squires. 2001. Metabolic activity of equine blastocysts cryopreserved in ethylene glycol and galactose. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 57-59.
Seidel, G.E., Jr. 2001. Cloning (Genetics). McGraw-Hill Yearbook of Science and Technology, pp. 98-100.
Seidel, G.E., Jr. 2001. Sexing bovine sperm. Proc. 34th Ann. Mtg. Am. Assoc. Bovine Practitioners, pp. 106-108 and Reprinted in substantially similar form in Ann. Proc. Korean Societies of Animal Reproduction (2001), pp. 6-10.
Seidel, G.E., Jr. 2001. Procedures for sexing bovine sperm. In: Advances in Beef Cattle Science, K.A. Beauchemin and D.H. Crews, Jr. (Eds). Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta, pp. 235-239.
Seidel, G.E., Jr. and J.F. Hasler. 2001. El sexado de semen bovino. Proc. 4b Simposio Internacional de Reproduccion Animal, pp. 25-30.
CONNECTICUT
Dinnyes, A., X.C. Tian, and X. Yang. 2002. Cloning of Rabbits. In (J.B. Cibelli, R.P. Lanza, K.H.S. Campbell, and M.D. West, Eds.) Principles of Cloning, Academic Press, San Diego, CA (in press).
Liu, L., M. Deng, X.C. Tian, and X. Yang. 2002. Principles and practice of oocyte activation in mammals. In (Daulat Talsiani, Ed.) Introduction to Mammalian Reproduction (in press).
ILLINOIS
Hickman, Debra. 2001. Efficacy of In Vitro Culture of Murine Preimplanation Embryos in Static And Dynamic Media Environments. M.S. Thesis, University of Illinois at Urbana-Champaign, 76 pgs.
Malusky, Samantha. 2001. Cloning and Sequence Analysis of Porcine Sucrase-Isomaltase Promoter and Transmembrane Region. M.S. Thesis, University of Illinois at Urbana-Champaign, 64 pgs.
Miller, D.J. and Wheeler, M.B. 2001. Cloning dairy cattle: How is it done and what is the future? Illinois Dairy Report: Focus on the Future, Illinois Agricultural Experiment Station pgs 28-30.
Noble, M.S., Wheeler, M.B., Hurley, W.L. 2001. Association of total milk solids intake and litter growth: Data from a-lactalbumin transgenic sows. Illinois Swine Report: Illinois Agricultural Experiment Station, pp. 131-133.
Raty, Stephannie. 2001. Design and Testing of Microfluidic Devices for Static Culture of Preimplantation Mammalian Embryos. M.S. Thesis, University of Illinois at Urbana-Champaign, 70 pgs.
IOWA
Sieren, K.R. 2001. The Use of BoviPure for the Separation of X and Y Chromosome-Bearing Bovine Sperm Cells. M.S. Thesis, Iowa State University, 61 pgs.
Sieren, K.R. and C.R. Youngs. 2001. Assessment of BoviPure for the in vitro production of bovine embryos. Beef Research Report, Iowa State University, A.S. Leaflet R1768, pp. 142-144.
Youngs, C.R. 2001. Cattle breeding in the 21st century. Proc. of the 30th Annual Cornbelt Cow-Calf Conference, February 24, Ottumwa, IA, pp. 1-9.
Youngs, C.R. 2001. Sheep and goat reproduction. Proc. of the Student American Veterinary Medical Association Symposium, March 8-10, Ames, IA, pp. 122-128.
Youngs, C.R., Ed. 2001. Proc. of the International Conference Commemorating the 50th Anniversary of the First Successful Embryo Transfer in the Pig. Theriogenology 56(8):1285-1369.
LOUISIANA
Bellow, M.S., B.A. Didon, D.L. Davis, C. Murphy, C.E. Ferguson, R.A. Godke and B.N. Day. 2001. A procedure for nonsurgical transvaginal ultrasound-guided oocyte aspiration in the sow. Cook Veterinary Symposium on Transvaginal Oocyte Aspiration/In Vitro Fertilization in the Pig (Omaha, Nebraska). pp. 28-29.
Carter, J.A., M.S. Bellows, M. Meintjes, O. Perez, E. Ferguson and R.A. Godke. 2001. Trans-vaginal ultrasound-guided oocyte aspiration in farm animals for in vitro embryo production. Cook Veterinary Symposium on Transvaginal Oocyte Aspiration/In Vitro Fertilization in the Pig (Omaha, Nebraska). pp. 17-27.
Ferguson, C.E., O. Perez, C. Airhart and R. A. Godke. 2001. Estrous synchronization using MGA or altrenogest in crossbred beef heifers. LA. Beef Cattle Res. Rep. 31:31-33.
Fernandez, J.M., J.E. Miller, and R.A. Godke (2001) Conservation plan for the American Gulf Coast Native sheep. In: Proc. Vth Congreso Iberoamericano de Razas Autoctonas y Criollas, Havana, Cuba (November 28 - December 1, 2000). pp. 42-43.
Gentry, G.T., R.P. Del Vechio, and R.A. Godke. 2001. Estrual behavioral patterns in prostaglandin-induced and naturally cycling recipient females from a commercial embryo transfer facility. LA. Beef Cattle Res. Rep. 31:5-8.
Gentry, G.T., Jr., R.P. Del Vecchio D. Faber and R.A. Godke. 2001.The effect of prostaglandin F2 (Lutalyse.) on the behavioral estrus patterns in beef cattle. Louisiana Cattleman (June Issue). 2 pages.
Godke, R.A. New assisted reproductive technologies: Their potential for use in large animals. Proc. Zool. Biotechnol Prod. Conf. (Portugal) (In Press).
Perez, O., R. Richard III, H.L. Green and R.A. Godke. 2001. Ultrasound-guided transvaginal oocyte recovery from postpartum beef cows. LA. Beef Cattle Res. Rep. 31:9-11.
OREGON
Paul, K. B. 2001. Membrane-type Matrix Metalloproteinase and Inhibitor Expression in Sheep Embryos and Uterus. M.Sc. Thesis, Oregon State University, Corvallis, OR.
UTAH
Wang, S., K.E. Panter, R.C. Evans and T.D. Bunch. 2001. Bovine embryo development in vitro in medium containing pokeweed mitogen or phytohemagglutinin. Proceedings Western Section Amer. Soc. Anim. Sci. 52: 436-439.
Wang, S., K.E. Panter, L.F. James, D.R. Gardner, R.C. Evans and T.D. Bunch. 2001. In vitro development of bovine embryos treated with isocupressic acid (ICA), a pine needle abortifacient toxin. Proceedings Western Section Amer. Soc. Anim. Sci. 52: 440-443.
White, K. L., T.D. Bunch, S. Mitalipov and W.A. Reed. 2001. Application of ARTs and nuclear transfer in exotic and endangered species. In (D.P. Wolf and M. Zelinski-Wooten, Eds.) Comparative Endocrinology: Assisted Fertilization and Nuclear Transfer in Mammals. Humana Press Inc., Totowa, NJ, pp. 229-251.
WISCONSIN
Rutledge, J.J. 2001. Biotechnology enables matching global genetic resources with local environmental niches in the Tribe Bovini (true callte). Proc. Symp. Int‘l. Conf. on Agricultural Science and Technology, November 7-9, Beijing, China, pp.
ABSTRACTS:
ARKANSAS
Bilby, T.R. and R.W. Rorie. 2001. Effectiveness of maturational treatments to block porcine oocyte germinal vesicle breakdown without reducing subsequent nuclear maturation and cleavage. Theriogenology 55:463.
Bilby, T.R. and R.W. Rorie. 2001. Effects of sequence and timing of gonadotropin supplementation on porcine oocyte maturation and subsequent cleavage in vitro. J Anim Sci 79(Suppl 2):20.
Burke, J.M., R.W. Rorie, G.A. Perry, and D.H. Keisler. 2001. Changes in ovarian function in mature beef cows grazing endophyte-infected (E+) tall fescue. Biol. Reprod. 64(Suppl. 1):344.
CALIFORNIA
Ducummon, C. and T. Berger. 2001. Cytotoxic necrotizing factor, a bacterial activator of the monomeric G?protein Rho?A, enhances spontaneous acrosome reactions in porcine sperm. Biol. Reprod. 64(Suppl 1):300.
Sartini, B.L. and T. Berger. 2001. Localization of DRKD sperm protein on boar sperm. Biol. Reprod. 64(Suppl 1):277.
Williams, J., B.L. Sartini, and T. Berger. 2001. Flow cytometric analysis of boar sperm mitochondrial function with JC-1. Biol. Reprod. 64(Suppl 1):301-302.
COLORADO
DeLille, A.J.A.E., R.V. Anthony and G.E. Seidel, Jr. 2001. Characteristics of placental and fetal tissues from Day 75 nuclear cloned bovine pregnancies. Theriogenology 55:263.
Lu, K.H., T.K. Suh and G.E. Seidel, Jr. 2001. In vitro fertilization of bovine oocytes with flow-cytometrically sorted and unsorted sperm from different bulls. Theriogenology 55:431.
Seidel, G.E., Jr. and L.J. Maclellan. 2001. Effect of peptide Fert Plus on bovine in vitro fertilization and development. Theriogenology 55:437.
CONNECTICUT
Deng, M. and X. Yang. 2001. Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection. Theriogenology 54:502.
Enright, B.P., M. Taneja, D. Schreiber, J. Riesen, X.C. Tian, and X. Yang. 2001. Puberty and early follicular dynamics in adult somatic cell derived cloned heifers. Theriogenology 54:267.
Lee, J.-W., B.-S. Jeong, J. Riesen, T. Hoagland, and X. Yang. 2001. Fertilization and embryonic development following injection of frozen-thawed spermatozoon into in vitro matured porcine oocytes. Theriogenology 54:506.
Taneja, M., R. French, H. Levine, D. Tauro-Miller, and X. Yang. 2001. Clinical and pathological status of cloned calves born pre-term. Theriogenology 54:293.
ILLINOIS
Chan, N.G., S. Raty , H.C. Zeringue, D.J. Beebe, M.B. Wheeler. 2001. Development of microfabricated devices for embryo production: Embryo biocompatibility. Theriogenology 55:332.
Clark, S.G., J. Davis, D. J. Beebe, M.B. Wheeler. 2001. Biocompatibility of porcine sperm cells in polydimethylsiloxane (PDMS). Theriogenology 55:421.
Hassun, P., Silva, I.G., Wheeler, M.B., Baracat, E.C., Freitas, V., Verreschi, I.T.N. 2001. Analysis of polymorphisms in the QM gene in women with premature ovarian dysfunction. Brazilian Human Genome Meeting.
Monaco, M.H., Cook, J.B., Tappenden, K., Wheeler, M.B., Donovan, S. M. 2001. Intestinal lactase activity is increased in piglets suckling IGF-transgenic sows. The FASEB Journal: A644.
Raty , S., J. Davis , M.B. Wheeler, D.J. Beebe. 2001. Culture in microchannels enhances in vitro culture of preimplantation mouse embryos. Theriogenology 55:241.
Walters, E.M., D.J. Beebe, M.B. Wheeler. 2001. In vitro maturation of pig oocytes in PDMS and silicon microfludic devices. Theriogenology 55:497.
IOWA
Dikeman, M.A., D.R. Strohbehn, and C.R. Youngs. 2001. Development of a web-based scheduling program for synchronization of estrus in cattle. J. Anim. Sci. 79(Suppl.2):15.
Funk, D.J. and C.R. Youngs. 2001. Embryo transfer, in vitro fertilization, cloning and transgenic production in farm animals: A review. Proc. of the Student American Veterinary Medical Association Symposium, March 8-10, Ames, IA, p. 89
Pugh, M.L., M. Pence, J.N. Caamaqo, S. Robbe, L.L. Timms, J.U. Thomson, and C.R. Youngs. 2001. Use of a vaginal mucus electrical resistance probe to alter insemination time and calf gender distribution in beef heifers. J. Anim. Sci. 79(Suppl. 2):66-67.
Pugh, M.L., K.R. Sieren, L.L. Timms, and C.R. Youngs. 2001. Use of a vaginal mucus electrical resistance probe to alter insemination time and calf gender distribution in dairy heifers. J. Dairy Sci. 84(Suppl. 1):64.
Sieren, K.R. and C.R. Youngs. 2001. Evaluation of BoviPure for the in vitro production of bovine embryos. Theriogenology 55:438.
LOUISIANA
Bartels, P., Y. Friedmann, K. Lubbe, D. Mortimer, L.A. Rasmussen and R.A. Godke. 2001. The live birth of an eland (Taurotragus oryx) calf following estrous synchronization and artificial insemination using frozen-thawed epididymal sperm. Theriogenology 55:381.
Bellow, M.S., B.A. Didon, D.L. Davis, C. Murphy, C.E. Ferguson, R.A. Godke and B.N. Day. 2001. A procedure for nonsurgical transvaginal ultrasound-guided oocyte aspiration in the sow. Theriogenology 55:528.
Chen, L.H., E. Behboodi, B.C. Reggio, H. Liem, M.M. Destrempes, H.L. Green, C.A. Ziomek, R.S. Denniston, Y. Echelard, R.A. Godke and H.M. Meade. 2001. Production of transgenic goats from a transfected fibroblast cell line. Theriogenology 55:259.
Cochran, R., M. Meintjes, D. Hylan, M. Sansinena, R. S. Denniston D. Paccamonti and R.A. Godke. 2001. Follicle growth in mares for oocyte production. In: From Epididymis to Embryo. Havemeyer Foundation Workshop. (October 18-21, 2001) New Orleans. p. 21.(Abstract)
Gentry, G.T., R.P. Del Vecchio and R.A. Godke. 2001. Effect of the presence of the male on characteristics of estrus of synchronized and naturally cycling crossbred heifers. J. Anim. Sci., Southern Section (Abstr.).
Paccamonti, D., M. Sansinena, C. Cochran, M. Meintjes, B. Reggio, R. S. Denniston, C. Pinto, B. Eilts and R.A.Godke. 2001. Developing the intracytoplasmic sperm injection (ICSI) procedure for the horse. In: From Epididymis to Embryo. Havemeyer Foundation Workshop. (October 18-21, 2001) New Orleans. p. 26. (Abstract).
Perez, O., A. Boediono, E. Ferguson, C. Airhart, R. Richard III and R.A. Godke. 2001. Oocyte and embryo production from FSH-treated postpartum beef cows shortly after calving. Theriogenology 55:501.
Poleo, G. A., R. S. Denniston, B. Reggio, R. A. Godke and T.R. Tiersch. 2001. Intracytoplasmic sperm injection (ICSI) in Zebrafish eggs. Proc World Aquaclture Soc. (Orlando, FL).
Songsasen, N., I. Yu, D. Paccamonti, B.E. Eilts, S. Mourton, R.A. Godke and S.P. Leibo. 2001. Osmotic sensitivity of canine spermatozoa. Proc. 37th Annual Mtg. Society of Cryobiology (Boston). p. 350, No. 60.
Songsasen, N., I. Yu, D. Paccamonti, B.E. Eilts, S. Mourton, R.A. Godke and S.P. Leibo. 2001. Survival and binding ability of canine spermatozoa stored at 4*C. Proc. 37th Annual Mtg. Society of Cryobiology (Boston). P. 361,No. 61.
Taylor, P.J., S. Taylor, A.N. James, R.S. Denniston and R.A. Godke. 2001. Alpaca offspring born after cross species embryo transfer to llama recipients. Theriogenology 55:401.
UTAH
Panter, K.E., S.Wang, L.F. James, W. Garfield, R.C. Evans and T.D. Bunch. 2001. Effects of Cyclopamine and synthetic analog on bovine oocyte maturation in vitro. Theriogenology 55:491.
Wang, S., K.E. Panter, L.F. James, W. Garfield, R.C. Evans and T.D. Bunch. 2001. The effects of steroidal alkaloids from Veratrum californium on bovine embryo development in vitro. Theriogenology 55:345.
WISCONSIN
Al-Katanani, Y.M., M. Drost, R.L. Monson, J.J. Rutledge, C.E. Krininger III, C.J. Block, and P.J. Hansen. 2001. Pregnancy rates in lactating dairy cattle following timed embryo transfer under heat stress condition. J. Anim. Sci. (Suppl): (in press).
MISCELLANEOUS PUBLICATIONS:
COLORADO
Seidel, G.E., Jr. 2001. What about sexed semen? Hoard‘s Dairyman 146:322.
Seidel, G.E., Jr. 2001. Sexing bovine sperm. Handout for National Association of Animal Breeders Mexican Seminar Series.
CONNECTICUT
Tian, X.C. 2001. How safe is milk from cloned cows? University of Connecticut College of Agriculture Newsletter.
Yang, X. and X.C. Tian. 2001. Don‘t ban therapeutic cloning. Commentary in Hartford Currant, December 2, 2001.
IOWA
Youngs, C.R. 2001. A new millennium for the Jersey breed. Iowa Jersey Today 7(2):5.
Youngs, C.R. 2001. The Magnificent Seven... hormones controlling reproduction in the cow (Part I). Iowa Jersey Today 7(3):6.
Youngs, C.R. 2001. Use of a vaginal mucus electrical resistance probe as a breeding tool for dairy cattle. Swiss Valley Dairyman, November issue, pp. 6-7.
ARKANSAS
Brown, A H, D W Kellogg, Z B Johnson, R W Rorie, W K Coblentz, B A Sandelin, K E Lesmeister and W R Jackson. 2001. Growth, luteal activity and pregnancy rates of three breed types of dairy heifers in a forage based development program. Prof. Anim. Sci. 17:20-26
Burke, J.M., R.W. Rorie and G.A. Perry. 2002. Changes in ovarian function in mature beef cows grazing endophyte-infected tall fescue. Theriogenology (in press).
Burke, J M, R W Rorie, E L Piper and W G Jackson. 2001. Reproductive responses to grazing endophyte-infected tall fescue in postpartum beef cows. Theriogenology 56:357-369.
Kreider, D L, R Rorie, D Brown, F Miller and S Wright. 2001. Enhancement of ovulation rate and litter size in gilts. J. Anim. Sci. 79:1691-1696.
Melnychuk, V.L., M.A. Cooper, R.W. Rorie, J.D. Kirby and N.B. Anthony. 2001. Characterization of ovary status using ultrasound techniques. J. Poultry Sci. (in press)
Rorie, R.W., T.R. Bilby, and T.D. Lester. 2002. Application of electronic estrus detection technology to reproductive management of cattle. Theriogenology (in press).
CALIFORNIA
Berger, T. and Dally, M. 2001. Do sire-dam interactions contribute significantly to fertility comparisons in heterospermic insemination trials? Theriogenology 56:535-543.
Bertolini, M., J.B. Mason, S.E. Beam, G.F. Carneiro, M.L. Sween, D.J. Kominek, A.L. Moyer, T.R. Famula, R.D. Sainz and G.B. Anderson. 2002. Morphology and morphometry of in vitro-and in vitro-produced concepti from early pregnancy to term and association with high birth weights. Theriogenology (in press).
Carneiro, G., P. Lorenzo, C. Pimental, L. Pagoraro, M. Bertolini, B. Ball, G. Anderson and I. Liu. 2001. The influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol and fetal calf serum and in vitro maturation and parthenogenic development in equine oocytes. Biology of Reproduction 65:899-905.
Gravance, C.G., Garner, D.L., Miller, M.G. and Berger, T. 2001. Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function. Reprod. Tox. 15:5-10.
Jelks, K., Berger, T., Horner, C., and Miller, M.G. 2001. Chlorohydrin induced changes in sperm fertilizing ability in the rat: association with diminished sperm ATP levels and motility. Reprod. Tox. 15:11-20.
COLORADO
Choi, Y.H., E.M. Carnevale, G.E. Seidel, Jr. and E.L. Squires. 2001. Effects of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology 56:661-670.
Choi, Y.H., Y.G. Chung, G.E. Seidel, Jr. and E.L. Squires. 2001. Developmental capacity of equine oocytes matured and cultured in equine trophoblast- conditioned media. Theriogenology 56:329-339.
Landim-Alvarenga, F.C., M.A. Alvarenga, G.E. Seidel, Jr., E.L. Squires and J.K. Graham. 2001. Penetration of zona-free hamster, bovine and equine oocytes by stallion and bull spermatozoa pretreated with equine follicular fluid, dilauroylphosphatidylcholine or calcium ionophore A23187. Theriogenology 56:937-953.
Lane, M., M.K. O‘Donovan, E.L. Squires, G.E. Seidel, Jr. and D.K. Gardner. 2001. Assessment of metabolism of equine morula and blastocysts. Molec. Reprod. Develop. 59:33-37.
Oberstein, N., M.K. O‘Donovan, J.E. Bruemmer, G.E. Seidel, Jr., E.M. Carnevale and E.L. Squires. 2001. Cryopreservation of equine embryos by open pulled straw, cryoloop, or conventional slow cooling methods. Theriogenology 55:607-613.
Seidel, G.E., Jr. 2001. Cloning, transgenesis, and genetic variance in animals. Cloning and Stem Cells 4:251-256.
Coutinho da Silva, M.A., E.M. Carnevale, L.J. Maclellan, G.E. Seidel, Jr. and E.L. Squires. 2002. Effect of time of oocyte collection and site of insemination on oocyte transfer in mares. J. Anim. Sci. (in press).
Li, G.-P., G.E. Seidel, Jr. and E.L. Squires. 2002. Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa. Theriogenology (in press).
Li, G.-P., G.E. Seidel, Jr. and E.L. Squires. 2002. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology (in press).
CONNECTICUT
Deng, M.Q. and X. Yang. 2001. Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection. Molec. Reprod. Devel. 58:1-6.
Dinnyes, A., L. Liu, Y. Dai, M. Barber, J. Xu, P. Zhou, and X. Yang. 2001. Development of cloned embryos from adult rabbit fibroblast cells. Biol. Reprod. 64:257-263.
Jeong, B.S. and X. Yang. 2001. Cysteine, glutathione, and percoll treatments improve porcine oocyte maturation and fertilization in vitro. Molec. Reprod. Devel. 59:330-335.
Tian, XC, C. Kubota, and X. Yang. 2001. Cloning of aged animals - a medical model for tissue and organ regeneration. Trends in Cardiovascular Medicine 11:313-317.
Xu, J. and X. Yang. 2001. Telomerase activity in early bovine embryos derived from pathenogenetic activation and nuclear transfer. Biol. Reprod. 64:770-774.
Yang, X. and J. Xu. 2001. Developmental changes of telomerase in oocytes and embryos. Advances in Reproduction 6:63-68.
Enright, B., M. Taneja, J. Riesen, X.C. Tian, J. Fortune, and X. Yang. 2002. Normal onset of puberty in cloned heifers of an aged Holstein cow. Biol. Reprod (in press).
Govoni, K.E., X.C. Tian, G.M. Kazmer, M. Taneja, B.P. Enright, A.L. Rivard, X. Yang, and S.A. Zinn. 2002. Age-related changes of the somatotropic axis in cloned Holstein calves. Biol. Reprod. (in press).
Liu, J.L., L.-Y. Sung, M. Barber, and X. Yang. 2002. Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes. Biol. Reprod. (in press).
Tian, X.C., P. Lonergan, A.E.O. Evans, and X. Yang. 2002. Dynamics of MPF and MAPK in oocytes matured in vitro for 20 vs. 40 hours. Molec. Reprod. Devel. (in press).
ILLINOIS
Boston, W.S., G.T. Bleck, M.B. Wheeler, D.J. Miller. 2001. The effects of increased expression of alpha-lactalbumin in transgenic mice on milk yield and pup growth. J. Dairy Sci. 84:620-622.
Donovan, S. M. , M. H. Monaco, G. T. Bleck, J. B. Cook, M. S. Noble, W.L. Hurley and M.B. Wheeler. 2001. Transgenic over-expression of bovine alpha-lactalbumin and human insulin-like growth factor-I in porcine mammary gland. J. Dairy Sci. 84 (E. Suppl.): E216-E222. (http://www.adsa.org/jds/papers/2001/jds_es216.pdf)
Glasgow, I.K., Zeringue, H.C., Beebe, D.J., Choi, S.-J., Lyman, J., Chan, N.G., Wheeler, M.B. 2001. Handling individual mammalian embryos using microfluidics. IEEE Transactions on Biomedical Engineer 48:570-577.
Wheeler, M.B. And Walters, E.M., 2001. Transgenic technology and applications in swine. Theriogenology 56:1345-1370.
Zeringue, H., M. B. Wheeler and D. J. Beebe, 2001. Removal of cumulus from mammalian oocytes using microfluidic techniques. Biomedical Microdevices 3:219-224.
IOWA
Youngs, C.R. 2001. Factors influencing the success of embryo transfer in the pig. Theriogenology 56:1311-1320.
LOUISIANA
Han, Y., M. Meintjes, K. Graff, R. Denniston, L. Zhang, C. Ziomek and R.A. Godke. 2001. Production of fresh and frozen-thawed transplant offspring from latter stage IVF-derived caprine embryos. Vet. Rec. 149:714-716.
Meintjes, M., B. Eilts, K. Graff, R. Denniston, D. Paccamonti and R. Godke. 2001. Transvaginal intrafollicular sperm cell injection in the cyclic mare. Internat‘l. Symp. Equine Embryo Transf. Monograph 3:39-40.
Murakami, M., A. Boediono, O. Perez, E. Ferguson, R. S. Denniston, E. Behboodi and R. A. Godke. 2001. The use of adult bovine skin fibroblasts cultured at different intervals prior to nuclear transfer. Arch. Anim. Breeding 44:175-177.
Poleo, G.A., R. S. Denniston, B. Reggio, R. A. Godke and T. R. Tiersch. 2001. Fertilization of eggs of Zebrafish (Danio rerio) by intracytoplasmic sperm injection. Biol. Reprod. 65:321-326.
Reggio, B.C., A.N. James, H. L. Green, W. G. Gavin, E. Behboodi, Y. Echelard and R. A. Godke. 2001. Cloned transgenic offsring in goats resulting from somatic cell nuclear transfer in goats: Oocytes derived from FSH-stimulated and nonstimulated ovaries. Biol Reprod. 65:1528-1533.
Reggio, B.C., M. Sansinena, R.A. Cochran, A. Guitreau, J.A. Carter, R.S. Denniston and R.A. Godke. 2001. Nuclear transfer embryos in the mare. Internat‘l. Symp. Equine Embryo Transf. Monograph 3:45-46.
Boediono, A., T. Suzuki and R.A. Godke. The development of in vitro-derived hybrid and straightbred beef cattle embryos during in vitro culture. Anim. Reprod. Sci. (in press).
Carter, J.A., S. Bellow, M. meintjes, O. Perez, E. Ferguson and R. A. Godke. Transvaginal ultrasound-guided oocyte aspiration for production of embryos in vitro. Arch. Anim. Breeding (in press).
Cochran, R., M. Meintjes, D. Hylan, M. Sansinena, R. S. Denniston D. Paccamonti and R.A. Godke. Follicle growth in mares for oocyte production. In: From Epididymis to Embryo. Havemeyer Foundation. (in press)
Han, Y., Y. Han, R.S. Denniston, A. Boediono, C. Ziomek and Godke. Development of IVF-derived caprine embryos with four different somatic cell co-cultures. Small Ruminant Res. (in press).
Murakami, M., S. Ideguchi, M. Fahrudin and R.A. Godke. Bovine nuclear transplant using fluorescent embryos after microinjection with the EGFP gene. Anim. Reprod. Develop. (in press).
Pinto, C.R.F., D.L. Paccamonti, B.E. Eilts, C.R. Short and R.A. Godke. Effect of nitric oxide synthetase inhibitors on ovulation in hCG-stimulated mares. Theriogenology (in press).
Sansinena, M., Paccamonti, D., C. Cochran, M. Meintjes, B. Reggio, R. S. Denniston, C. Pinto, B. Eilts and R.A.Godke. Developing the intracytoplasmic sperm injection (ICSI) procedure for the horse. In: From Epididymis to Embryo. Havemeyer Foundation. (in press).
Songasen, N., I. Yu, S. Murton, D. Paccamonti, B.E.Eilts, R. A. Godke and S.P. Leibo. Osmotic sensitivity of Canine Spermatozoa. Cryobiology (in press).
Yu, I.J., N. Songsasen, R.A. Godke and S.P. Leibo. Differences among male dogs in response of their spermatozoa cryopreservced at various coolling and warnimg rates. Cryobiology (In press).
OREGON
Al-Aghbari, A.M. and A.R. Menino, Jr. 2002. Survival of oocytes recovered from vitrified sheep ovarian tissues. Anim. Reprod. Sci.(in press)
Schilperoort-Haun, K.R. and A.R. Menino, Jr. 2002. Evaluation of extracellular matrix proteins and tissue inhibitor of matrix metalloproteinases-2 on bovine inner cell mass outgrowth in vitro. In Vitro Cell. Dev. Biol.-Animal (in press).
UTAH
Kitiyanant, Y., J. Saikhun, B. Chrisalee, K.L. White and K. Pavasuthipaisit. 2001. Somatic Cell Cloning in Buffalo (Bubalus bubalis): Effects of interspecies cytoplasmic recipients and activation procedures. Cloning and Stem Cells 3:97-104.
Thonabulsombat, C., W.A. Reed, J.D. Morrey, K. Bates, R. Smart and K.L. White. 2001. Transgenic mice carrying a gene for green fluorescent protein fused to a lytic peptide, Shiva 1, under control of the bovine 8-Casein regulatory region. Transgenics 3:183-197.
Viets, L.N., K.D. Campbell and K.L. White. 2001. Pathways involved in RGD-mediated calcium transients in mature bovine oocytes. Cloning and Stem Cells 3:105-113.
Wang, S., W.C. Foote, D.L. Sutton, A. Maciulis, J.M. Miller, R.C. Evans, G.R. Holyoak, J.W. Call, T.D. Bunch, W.D. Taylor and M.R. Marshall. 2001. Preventing experimental vertical transmission of scrapie by embryo transfer. Theriogenology. 56:315-327.
Wang, S., K.E. Panter, R.C. Evans, and T.D. Bunch. 2001. The effects of pokeweed mitogen (PWM) and phytohemagglutinin (PHA) on bovine oocyte maturation and embryo development in vitro. Anim. Reprod. Sci. 67:215-220.
WASHINGTON
Bosch, P., J.M. deAvila, J.E. Ellington, and R.W. Wright, Jr. 2001. Heparin and Ca2+-free medium can enhance release of bull sperm attached to oviductal epithelial cell monolayers. Theriogenology 56:247-260.
WISCONSIN
Rutledge, J.J. 2001. Greek temples, tropical kine and recombination load. Livestock Production Science 68:171-179.
Rutledge, J.J. 2001. Use of embryo transfer and IVF to bypass effects of heat stress. Theriogenology 55:105-111.
Al-Katanani, Y.M., M. Drost, R.L. Monson, J.J. Rutledge, C.E. Krininger III, C.J. Block, W.W. Thatcher, and P.J. Hansen. 2002. Pregnancy rates following timed embryo transfer with fresh or vitrified in vitro produced embryos in lactating dairy cows under heat stress conditions. Theriogenology (in press).
Men, H., R.L. Monson, and J.J. Rutledge. 2002. Effect of maturation process and maturation protocols on bovine oocyte freezing tolerance. Theriogenology (in press).
BOOKS, CHAPTERS IN BOOKS, PROCEEDINGS, AND THESES:
CALIFORNIA
Berger, T. and Dally, M. 2001. Is there a sire-dam interaction in sperm fertilizing potential? California Agriculture 55:25.
COLORADO
Choi, Y.H., G.E. Seidel, Jr., S. Boyazoglu and E.L. Squires. 2001. Polyvinylalcohol is superior to bovine serum albumin in equine IVF medium. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 28-29.
Garner, D., J. Schenk and G. Seidel, Jr. 2001. Chromatin stability in sex-sorted sperm. Proc. VII Int. Congr. Androl., pp. 3-7.
Landim-Alvarenga, F.C., S.E.A. Boyazoglu, G.E. Seidel, Jr. and E.L. Squires. 2001. Effect of fetuin on zona hardening and the distribution of cortical granules in equine oocytes matured in vitro. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 26-27.
Oberstein, N., M.K. O‘Donovan, J.E. Bruemmer, M. Lane, G.E. Seidel, Jr., E.M. Carnevale and E.L. Squires. 2001. Cryopreservation of equine embryos by open pulled straw, cryoloop or conventional slow cooling methods. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 54-56.
O‘Donovan, M.K., N. Oberstein, J.E. Bruemmer, M. Lane, G.E. Seidel, Jr., D.K. Gardner and E.L. Squires. 2001. Metabolic activity of equine blastocysts cryopreserved in ethylene glycol and galactose. Proc. 5th Int. Symp Equine Embryo Transfer, T. Katila and J.F. Wade (Eds), Havemeyer Foundation Monograph Series No. 3, pp. 57-59.
Seidel, G.E., Jr. 2001. Cloning (Genetics). McGraw-Hill Yearbook of Science and Technology, pp. 98-100.
Seidel, G.E., Jr. 2001. Sexing bovine sperm. Proc. 34th Ann. Mtg. Am. Assoc. Bovine Practitioners, pp. 106-108 and Reprinted in substantially similar form in Ann. Proc. Korean Societies of Animal Reproduction (2001), pp. 6-10.
Seidel, G.E., Jr. 2001. Procedures for sexing bovine sperm. In: Advances in Beef Cattle Science, K.A. Beauchemin and D.H. Crews, Jr. (Eds). Agriculture and Agri-Food Canada Research Centre, Lethbridge, Alberta, pp. 235-239.
Seidel, G.E., Jr. and J.F. Hasler. 2001. El sexado de semen bovino. Proc. 4b Simposio Internacional de Reproduccion Animal, pp. 25-30.
CONNECTICUT
Dinnyes, A., X.C. Tian, and X. Yang. 2002. Cloning of Rabbits. In (J.B. Cibelli, R.P. Lanza, K.H.S. Campbell, and M.D. West, Eds.) Principles of Cloning, Academic Press, San Diego, CA (in press).
Liu, L., M. Deng, X.C. Tian, and X. Yang. 2002. Principles and practice of oocyte activation in mammals. In (Daulat Talsiani, Ed.) Introduction to Mammalian Reproduction (in press).
ILLINOIS
Hickman, Debra. 2001. Efficacy of In Vitro Culture of Murine Preimplanation Embryos in Static And Dynamic Media Environments. M.S. Thesis, University of Illinois at Urbana-Champaign, 76 pgs.
Malusky, Samantha. 2001. Cloning and Sequence Analysis of Porcine Sucrase-Isomaltase Promoter and Transmembrane Region. M.S. Thesis, University of Illinois at Urbana-Champaign, 64 pgs.
Miller, D.J. and Wheeler, M.B. 2001. Cloning dairy cattle: How is it done and what is the future? Illinois Dairy Report: Focus on the Future, Illinois Agricultural Experiment Station pgs 28-30.
Noble, M.S., Wheeler, M.B., Hurley, W.L. 2001. Association of total milk solids intake and litter growth: Data from a-lactalbumin transgenic sows. Illinois Swine Report: Illinois Agricultural Experiment Station, pp. 131-133.
Raty, Stephannie. 2001. Design and Testing of Microfluidic Devices for Static Culture of Preimplantation Mammalian Embryos. M.S. Thesis, University of Illinois at Urbana-Champaign, 70 pgs.
IOWA
Sieren, K.R. 2001. The Use of BoviPure for the Separation of X and Y Chromosome-Bearing Bovine Sperm Cells. M.S. Thesis, Iowa State University, 61 pgs.
Sieren, K.R. and C.R. Youngs. 2001. Assessment of BoviPure for the in vitro production of bovine embryos. Beef Research Report, Iowa State University, A.S. Leaflet R1768, pp. 142-144.
Youngs, C.R. 2001. Cattle breeding in the 21st century. Proc. of the 30th Annual Cornbelt Cow-Calf Conference, February 24, Ottumwa, IA, pp. 1-9.
Youngs, C.R. 2001. Sheep and goat reproduction. Proc. of the Student American Veterinary Medical Association Symposium, March 8-10, Ames, IA, pp. 122-128.
Youngs, C.R., Ed. 2001. Proc. of the International Conference Commemorating the 50th Anniversary of the First Successful Embryo Transfer in the Pig. Theriogenology 56(8):1285-1369.
LOUISIANA
Bellow, M.S., B.A. Didon, D.L. Davis, C. Murphy, C.E. Ferguson, R.A. Godke and B.N. Day. 2001. A procedure for nonsurgical transvaginal ultrasound-guided oocyte aspiration in the sow. Cook Veterinary Symposium on Transvaginal Oocyte Aspiration/In Vitro Fertilization in the Pig (Omaha, Nebraska). pp. 28-29.
Carter, J.A., M.S. Bellows, M. Meintjes, O. Perez, E. Ferguson and R.A. Godke. 2001. Trans-vaginal ultrasound-guided oocyte aspiration in farm animals for in vitro embryo production. Cook Veterinary Symposium on Transvaginal Oocyte Aspiration/In Vitro Fertilization in the Pig (Omaha, Nebraska). pp. 17-27.
Ferguson, C.E., O. Perez, C. Airhart and R. A. Godke. 2001. Estrous synchronization using MGA or altrenogest in crossbred beef heifers. LA. Beef Cattle Res. Rep. 31:31-33.
Fernandez, J.M., J.E. Miller, and R.A. Godke (2001) Conservation plan for the American Gulf Coast Native sheep. In: Proc. Vth Congreso Iberoamericano de Razas Autoctonas y Criollas, Havana, Cuba (November 28 - December 1, 2000). pp. 42-43.
Gentry, G.T., R.P. Del Vechio, and R.A. Godke. 2001. Estrual behavioral patterns in prostaglandin-induced and naturally cycling recipient females from a commercial embryo transfer facility. LA. Beef Cattle Res. Rep. 31:5-8.
Gentry, G.T., Jr., R.P. Del Vecchio D. Faber and R.A. Godke. 2001.The effect of prostaglandin F2 (Lutalyse.) on the behavioral estrus patterns in beef cattle. Louisiana Cattleman (June Issue). 2 pages.
Godke, R.A. New assisted reproductive technologies: Their potential for use in large animals. Proc. Zool. Biotechnol Prod. Conf. (Portugal) (In Press).
Perez, O., R. Richard III, H.L. Green and R.A. Godke. 2001. Ultrasound-guided transvaginal oocyte recovery from postpartum beef cows. LA. Beef Cattle Res. Rep. 31:9-11.
OREGON
Paul, K. B. 2001. Membrane-type Matrix Metalloproteinase and Inhibitor Expression in Sheep Embryos and Uterus. M.Sc. Thesis, Oregon State University, Corvallis, OR.
UTAH
Wang, S., K.E. Panter, R.C. Evans and T.D. Bunch. 2001. Bovine embryo development in vitro in medium containing pokeweed mitogen or phytohemagglutinin. Proceedings Western Section Amer. Soc. Anim. Sci. 52: 436-439.
Wang, S., K.E. Panter, L.F. James, D.R. Gardner, R.C. Evans and T.D. Bunch. 2001. In vitro development of bovine embryos treated with isocupressic acid (ICA), a pine needle abortifacient toxin. Proceedings Western Section Amer. Soc. Anim. Sci. 52: 440-443.
White, K. L., T.D. Bunch, S. Mitalipov and W.A. Reed. 2001. Application of ARTs and nuclear transfer in exotic and endangered species. In (D.P. Wolf and M. Zelinski-Wooten, Eds.) Comparative Endocrinology: Assisted Fertilization and Nuclear Transfer in Mammals. Humana Press Inc., Totowa, NJ, pp. 229-251.
WISCONSIN
Rutledge, J.J. 2001. Biotechnology enables matching global genetic resources with local environmental niches in the Tribe Bovini (true callte). Proc. Symp. Int‘l. Conf. on Agricultural Science and Technology, November 7-9, Beijing, China, pp.
ABSTRACTS:
ARKANSAS
Bilby, T.R. and R.W. Rorie. 2001. Effectiveness of maturational treatments to block porcine oocyte germinal vesicle breakdown without reducing subsequent nuclear maturation and cleavage. Theriogenology 55:463.
Bilby, T.R. and R.W. Rorie. 2001. Effects of sequence and timing of gonadotropin supplementation on porcine oocyte maturation and subsequent cleavage in vitro. J Anim Sci 79(Suppl 2):20.
Burke, J.M., R.W. Rorie, G.A. Perry, and D.H. Keisler. 2001. Changes in ovarian function in mature beef cows grazing endophyte-infected (E+) tall fescue. Biol. Reprod. 64(Suppl. 1):344.
CALIFORNIA
Ducummon, C. and T. Berger. 2001. Cytotoxic necrotizing factor, a bacterial activator of the monomeric G?protein Rho?A, enhances spontaneous acrosome reactions in porcine sperm. Biol. Reprod. 64(Suppl 1):300.
Sartini, B.L. and T. Berger. 2001. Localization of DRKD sperm protein on boar sperm. Biol. Reprod. 64(Suppl 1):277.
Williams, J., B.L. Sartini, and T. Berger. 2001. Flow cytometric analysis of boar sperm mitochondrial function with JC-1. Biol. Reprod. 64(Suppl 1):301-302.
COLORADO
DeLille, A.J.A.E., R.V. Anthony and G.E. Seidel, Jr. 2001. Characteristics of placental and fetal tissues from Day 75 nuclear cloned bovine pregnancies. Theriogenology 55:263.
Lu, K.H., T.K. Suh and G.E. Seidel, Jr. 2001. In vitro fertilization of bovine oocytes with flow-cytometrically sorted and unsorted sperm from different bulls. Theriogenology 55:431.
Seidel, G.E., Jr. and L.J. Maclellan. 2001. Effect of peptide Fert Plus on bovine in vitro fertilization and development. Theriogenology 55:437.
CONNECTICUT
Deng, M. and X. Yang. 2001. Full term development of rabbit oocytes fertilized by intracytoplasmic sperm injection. Theriogenology 54:502.
Enright, B.P., M. Taneja, D. Schreiber, J. Riesen, X.C. Tian, and X. Yang. 2001. Puberty and early follicular dynamics in adult somatic cell derived cloned heifers. Theriogenology 54:267.
Lee, J.-W., B.-S. Jeong, J. Riesen, T. Hoagland, and X. Yang. 2001. Fertilization and embryonic development following injection of frozen-thawed spermatozoon into in vitro matured porcine oocytes. Theriogenology 54:506.
Taneja, M., R. French, H. Levine, D. Tauro-Miller, and X. Yang. 2001. Clinical and pathological status of cloned calves born pre-term. Theriogenology 54:293.
ILLINOIS
Chan, N.G., S. Raty , H.C. Zeringue, D.J. Beebe, M.B. Wheeler. 2001. Development of microfabricated devices for embryo production: Embryo biocompatibility. Theriogenology 55:332.
Clark, S.G., J. Davis, D. J. Beebe, M.B. Wheeler. 2001. Biocompatibility of porcine sperm cells in polydimethylsiloxane (PDMS). Theriogenology 55:421.
Hassun, P., Silva, I.G., Wheeler, M.B., Baracat, E.C., Freitas, V., Verreschi, I.T.N. 2001. Analysis of polymorphisms in the QM gene in women with premature ovarian dysfunction. Brazilian Human Genome Meeting.
Monaco, M.H., Cook, J.B., Tappenden, K., Wheeler, M.B., Donovan, S. M. 2001. Intestinal lactase activity is increased in piglets suckling IGF-transgenic sows. The FASEB Journal: A644.
Raty , S., J. Davis , M.B. Wheeler, D.J. Beebe. 2001. Culture in microchannels enhances in vitro culture of preimplantation mouse embryos. Theriogenology 55:241.
Walters, E.M., D.J. Beebe, M.B. Wheeler. 2001. In vitro maturation of pig oocytes in PDMS and silicon microfludic devices. Theriogenology 55:497.
IOWA
Dikeman, M.A., D.R. Strohbehn, and C.R. Youngs. 2001. Development of a web-based scheduling program for synchronization of estrus in cattle. J. Anim. Sci. 79(Suppl.2):15.
Funk, D.J. and C.R. Youngs. 2001. Embryo transfer, in vitro fertilization, cloning and transgenic production in farm animals: A review. Proc. of the Student American Veterinary Medical Association Symposium, March 8-10, Ames, IA, p. 89
Pugh, M.L., M. Pence, J.N. Caamaqo, S. Robbe, L.L. Timms, J.U. Thomson, and C.R. Youngs. 2001. Use of a vaginal mucus electrical resistance probe to alter insemination time and calf gender distribution in beef heifers. J. Anim. Sci. 79(Suppl. 2):66-67.
Pugh, M.L., K.R. Sieren, L.L. Timms, and C.R. Youngs. 2001. Use of a vaginal mucus electrical resistance probe to alter insemination time and calf gender distribution in dairy heifers. J. Dairy Sci. 84(Suppl. 1):64.
Sieren, K.R. and C.R. Youngs. 2001. Evaluation of BoviPure for the in vitro production of bovine embryos. Theriogenology 55:438.
LOUISIANA
Bartels, P., Y. Friedmann, K. Lubbe, D. Mortimer, L.A. Rasmussen and R.A. Godke. 2001. The live birth of an eland (Taurotragus oryx) calf following estrous synchronization and artificial insemination using frozen-thawed epididymal sperm. Theriogenology 55:381.
Bellow, M.S., B.A. Didon, D.L. Davis, C. Murphy, C.E. Ferguson, R.A. Godke and B.N. Day. 2001. A procedure for nonsurgical transvaginal ultrasound-guided oocyte aspiration in the sow. Theriogenology 55:528.
Chen, L.H., E. Behboodi, B.C. Reggio, H. Liem, M.M. Destrempes, H.L. Green, C.A. Ziomek, R.S. Denniston, Y. Echelard, R.A. Godke and H.M. Meade. 2001. Production of transgenic goats from a transfected fibroblast cell line. Theriogenology 55:259.
Cochran, R., M. Meintjes, D. Hylan, M. Sansinena, R. S. Denniston D. Paccamonti and R.A. Godke. 2001. Follicle growth in mares for oocyte production. In: From Epididymis to Embryo. Havemeyer Foundation Workshop. (October 18-21, 2001) New Orleans. p. 21.(Abstract)
Gentry, G.T., R.P. Del Vecchio and R.A. Godke. 2001. Effect of the presence of the male on characteristics of estrus of synchronized and naturally cycling crossbred heifers. J. Anim. Sci., Southern Section (Abstr.).
Paccamonti, D., M. Sansinena, C. Cochran, M. Meintjes, B. Reggio, R. S. Denniston, C. Pinto, B. Eilts and R.A.Godke. 2001. Developing the intracytoplasmic sperm injection (ICSI) procedure for the horse. In: From Epididymis to Embryo. Havemeyer Foundation Workshop. (October 18-21, 2001) New Orleans. p. 26. (Abstract).
Perez, O., A. Boediono, E. Ferguson, C. Airhart, R. Richard III and R.A. Godke. 2001. Oocyte and embryo production from FSH-treated postpartum beef cows shortly after calving. Theriogenology 55:501.
Poleo, G. A., R. S. Denniston, B. Reggio, R. A. Godke and T.R. Tiersch. 2001. Intracytoplasmic sperm injection (ICSI) in Zebrafish eggs. Proc World Aquaclture Soc. (Orlando, FL).
Songsasen, N., I. Yu, D. Paccamonti, B.E. Eilts, S. Mourton, R.A. Godke and S.P. Leibo. 2001. Osmotic sensitivity of canine spermatozoa. Proc. 37th Annual Mtg. Society of Cryobiology (Boston). p. 350, No. 60.
Songsasen, N., I. Yu, D. Paccamonti, B.E. Eilts, S. Mourton, R.A. Godke and S.P. Leibo. 2001. Survival and binding ability of canine spermatozoa stored at 4*C. Proc. 37th Annual Mtg. Society of Cryobiology (Boston). P. 361,No. 61.
Taylor, P.J., S. Taylor, A.N. James, R.S. Denniston and R.A. Godke. 2001. Alpaca offspring born after cross species embryo transfer to llama recipients. Theriogenology 55:401.
UTAH
Panter, K.E., S.Wang, L.F. James, W. Garfield, R.C. Evans and T.D. Bunch. 2001. Effects of Cyclopamine and synthetic analog on bovine oocyte maturation in vitro. Theriogenology 55:491.
Wang, S., K.E. Panter, L.F. James, W. Garfield, R.C. Evans and T.D. Bunch. 2001. The effects of steroidal alkaloids from Veratrum californium on bovine embryo development in vitro. Theriogenology 55:345.
WISCONSIN
Al-Katanani, Y.M., M. Drost, R.L. Monson, J.J. Rutledge, C.E. Krininger III, C.J. Block, and P.J. Hansen. 2001. Pregnancy rates in lactating dairy cattle following timed embryo transfer under heat stress condition. J. Anim. Sci. (Suppl): (in press).
MISCELLANEOUS PUBLICATIONS:
COLORADO
Seidel, G.E., Jr. 2001. What about sexed semen? Hoard‘s Dairyman 146:322.
Seidel, G.E., Jr. 2001. Sexing bovine sperm. Handout for National Association of Animal Breeders Mexican Seminar Series.
CONNECTICUT
Tian, X.C. 2001. How safe is milk from cloned cows? University of Connecticut College of Agriculture Newsletter.
Yang, X. and X.C. Tian. 2001. Don‘t ban therapeutic cloning. Commentary in Hartford Currant, December 2, 2001.
IOWA
Youngs, C.R. 2001. A new millennium for the Jersey breed. Iowa Jersey Today 7(2):5.
Youngs, C.R. 2001. The Magnificent Seven... hormones controlling reproduction in the cow (Part I). Iowa Jersey Today 7(3):6.
Youngs, C.R. 2001. Use of a vaginal mucus electrical resistance probe as a breeding tool for dairy cattle. Swiss Valley Dairyman, November issue, pp. 6-7.