W1171: Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock

(Multistate Research Project)

Status: Inactive/Terminating

SAES-422 Reports

02/18/2005

ARKANSAS

Nihsen M.E., E.L. Piper, C.P. West, R.J. Crawford, Jr, T.M. Denard , Z.B. Johnson, C.A. Roberts, D.A. Spiers, C.F. Rosenkrans, Jr. 2004. Growth rate and physiology of steers grazing tall fescue inoculated with novel endophytes. J Anim Sci. 82:878-83.

Post, N.M., D.L. Kreider and R.W. Rorie. 2004. Timed insemination in beef heifers after synchronization of estrus with controlled intravaginal drug releasing (CIDR) device and mesengesterol acetate (MGA). Prof. Anim. Sci (accepted).

Post, N.M., D.L. Kreider, R.W. Rorie and T.D. Lester. 2004. Timed AI in postpartum beef cows using melengestrol acetate, PGF2alpha and GnRH. Prof. Anim. Sci (in press).

CALIFORNIA

Bertolini M., A.L.Moyer, J.B. Mason, C.A. Batchelder, K.A. Hoffert, L.R. Bertolini, G.F. Carneiro, S.L. Cargill, T.F. Famula, C.C. Calvert, R.D. Sainz and G.B. Anderson. 2004. Evidence of increased substrate availability in invitro-derived bovine foetuses and association with accelerated conceptus growth. Reproduction 128:341-354.

DuTeaux, S.B., T. Berger, R. A. Hess, B.L. Sartini, and M.G. Miller. 2004. Male reproductive toxicity of trichloroethylene: sperm protein oxidation and decreased fertilizing ability. Biol. Reprod. 70:1518-1526.

Maga, E.A., G. Sargent, H. Zeng, S. Pati, D. Zarling, S. M. Oppenheim, N.M.B. Collette, A.L. Moyer, J.S. Conrad-Brink, J. D. Rowe, R.H. BonDurant, G.B. Anderson, and J.D. Murray. Increased efficiency of transgenic livestock production. Transgenic Research (in press).

Reh, W. A., Maga, E. A., Collette, N. M. B., Moyer, A., Conrad-Brink, J. S., Taylor, S. J., DePeters, E. J., Oppenheim, S., Rowe, J. D., BonDurant, R. H., Anderson, G. B. and Murray, J. D. 2004. Using a stearoyl-CoA desaturase transgene to alter milk fatty acid composition. J. Dairy Sci. 87:3510-3514.

COLORADO

Bass, L.D., D.J. Denniston, L.J. Maclellan, P.M. McCue, G.E. Seidel, Jr. and E.L. Squires. 2004. Methanol as a cryoprotectant for equine embryos. Theriogenology 62:1153-1159.

Carnevale, E.M., M.A. Coutinho da Silva, L.J. Maclellan, G.E. Seidel, Jr. and E.L. Squires. 2004. Use of parentage testing to determine optimum insemination time and culture media for oocyte transfer in mares. Reproduction 128:623-628.

Coutinho da Silva, M.A., E.M. Carnevale, L.J. Maclellan, K.A. Preis, G.E. Seidel, Jr. and E.L. Squires. 2004. Oocyte transfer in mares with intrauterine or intraoviductal insemination using fresh, cooled and frozen stallion semen. Theriogenology 61:705-713.

Eldridge-Panuska, W.D., V. Caracciolo di Brienza, G.E. Seidel, Jr., E.L. Squires and E.M. Carnevale. 2005. Development of equine embryos in vivo post vitrification and recovery rates of embryos 6.5 days after ovulation. Theriogenology. In Press.

Garner, D.L. and G.E. Seidel, Jr. 2004. Applications of flow cytometry in animal reproduction. In: Flow Cytometry in Biotechnology, L. Sklar (ed). Oxford University Press (USA). In Press.

Kimura, K., L.D. Spete, M.P. Green, C.N. Murphy, G.E. Seidel, Jr. and R.M. Roberts. 2004. Sexual dimorphism and interferon-tau production by in vivo-derived bovine embryos. Molec. Reprod. Develop. 67:193-199.

Li, G.-P., G.E. Seidel, Jr. and E.L. Squires. 2004. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology 61:1077-1084.

Lindsey, A.C., D.D. Varner, G.E. Seidel, Jr., J.E. Bruemmer and E.L. Squires. 2005. Hysteroscopic or rectally guided, deep-uterine insemination of mares with spermatozoa stored at 18 h at either 5°C or 15°C prior to flow-cytometric sorting. Anim. Reprod. Sci. 85:125-130.

Lu, K.H. and G.E. Seidel, Jr. 2004. Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm. Theriogenology 62:819-830.

Seidel, G.E., Jr. 2004. Sexing of spermatozoa. In: Bovine Theriogenology, G. Bo and M. De La Soto (eds). Inter-Media, Buenos Aires. In Press.

Suh, T.K., J.L. Schenk and G.E. Seidel, Jr. 2005. High pressure damages sperm during flow sorting. Theriogenology. In Press.

Tubman, L.M., Z. Brink, T.K. Suh and G.E. Seidel, Jr. 2004. Characteristics of calves produced with sperm sexed by flow cytometry/cell sorting. J. Anim. Sci. 82:1029-1036.

CONNECTICUT

Liu JL, Sung LY, Du FL, Julian M, Jiang S, Barber M, Xu J, Tian X and Yang X. Differential Development of Rabbit Embryos Derived from Parthenogenesis and Nuclear Transfer. Mol Reprod Dev 68:58-64, 2004

Nedambale TL, Dinnyés A, Groen W, Dobrinsky JR, Tian X and Yang X . Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification. Theriogenology 62:437-449, 2004.

Lee J, Tian X and Yang X. Optimization of Parthenogenetic Activation Protocol in Porcine. Mol Reprod Dev 68:51-57. 2004

Ji-Long Liu, Hirokazu Kusakabe, Ching-Chien Chang, Hiroyuki Suzuki, David W Schmidt, Marina Julian, Robert Pfeffer, Charles L Bormann, X. Cindy Tian, R. Yanagimachi, and X. Yang. Freeze-dried sperm fertilization led to term development in rabbits. Biol Reprod 70:1776-1781. 2004.

Jiang L, Carter DB, Xu J, Yang X, Prather RS, Tian XC. Telomere Lengths in Cloned Transgenic Pigs. Biol Reprod 2004 70:1589-1593.

Chang CC, Nagy P, Abdelmassih R, Yang X, Tian XC. Morphology of the G2/M nuclei during somatic cell haploidization by GV stage mouse oocytes. Biol Reprod 70:752-758 2004.

X Yang. Therapeutic Cloning and Embryo-based Research on Biotechnology and Medicine: Opportunities and Challenges. Nature 2004; 428:210-212.

Kubota C, Tian XC, Yang X. Differential fertility in second and third generation clones of a prize breeding bull. Nature Biotechnology 2004; 22: 693-694.

Tian XC. Reprogramming of epigenetic inheritance by somatic cell nuclear transfer. RBMonline 2004; 8:501-508.

Zhang, S, Kubota C, Yang L, Zhang Y, Page R, ONeill M, Yang X, Tian XC. Genomic imprinting of H19 in naturally reproduced and cloned cattle. Biol Reprod 2004 (accepted).

Tian, XC, Kubota C, Sakashita K, Izaike Y, Okano R, Tabara N, Curchoe C, Jacob L, Zhang Y, Smith S, Bormann C, Andrew S, Yang X. Meat and Milk Compositions of Bovine Clones Compared with Matched Controls. Science, 2004 (full length research article, accepted).

Yang, X, XC Tian, W Fodor. 2004. Cattle call for gene targeting. Nature Genetic (invited News and Views, accepted).

Enright BP, Sung L-Y, Chang C-C, Yang X, Tian XC. Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2-deoxycytidine. Biol Reprod 2004 (accepted).

Sung L-Y, Du F, Xu J, Chang W, Jiang S, Tian XC, Yang X. The effect of albumin and sodium citrate on the development of in vitro produced bovine embryos under different oxygen tension. Reprod Nutr Dev 2004 (accepted).

Nedambale TL, Dinnyés A, Yang X, Tian XC. Bovine blastocyst development in vitro: Timing, sex and viability following vitrification. Biol Reprod (accepted), 2004.

ILLINOIS

Wheeler, M.B., Clark, S.G., Beebe, D.J. 2004. Developments in in vitro technologies for swine embryo production. Reprod. Fertil. Devel. 16:15-25.

Raty, S., Walters, E.M., Davis, J., Zeringue, H. C., Beebe, D. J., Rodriguez-Zas, S.L., M. B.Wheeler. 2004. Embryonic Development in the Mouse Enhanced by via Microchannel Culture. Lab on a Chip 4:186-190.

Fischer-Brown, A.E., Lindsey, B.R., Ireland, F.A., Northey, D.L., Monson, R.L., Clark, S.G., Wheeler, M.B., Kesler, D.J., Lane, S.J., Weigel, K.A., Rutledge, J.J. 2004. Day 14 bovine embryo morphology: evaluation tool and potential marker of viability following in vitro production and in vivo culture. Reprod. Fert. Devel. 16:787-793.

Zeringue, H.C., Wheeler, M.B., Beebe, D.J. 2004. A Microfluidic Method for Removal of the Zona Pellucida from Mammalian Embryos. Lab on a Chip, 5(advance web article October 14th, 2004) 108-110.

Reeder, A. L., Ruiz, M.O., Pessier, A., Brown, L.E., Levengood, J.M., Phillips, C.A., Wheeler, M.B., Warner, R.E., Beasley, V.R. 2004. Intersexuality and the cricket frog decline: Historic and geographic trends. Environmental Health Perspectives (in press).

Hartke, J.L., Monaco, M.H., Wheeler, M.B, and Donovan, S.M. 2004. Effect of a short-term fast on intestinal disaccharidase activity and villus morphology in piglets suckling insulin-like growth factor-I (IGF-I) transgenic sows. J.Animal Sci. (in press).

Donovan, S.M., J.L. Hartke, M.H. Monaco and M.B. Wheeler. 2004. Over-expression of IGF-I: Effects on piglet intestinal growth. J. Dairy. Sci 87:(E. Suppl.): E47-E54.

INDIANA

Herrick, J.R., P. Bartels, and R.L. Krisher. 2004. Cryopreservation and post-thaw evaluation of in vitro function in epididymal spermatozoa from four species of free-ranging African bovids. Biol. Reprod. 71:948-958.

Herrick, J.R., E. Behboodi, E. Memili, S. Blash, Y. Echelard, and R.L. Krisher. 2004. Effect of macromolecule supplementation during in vitro maturation of goat oocytes on developmental potential. Mol. Reprod. Dev. 69:338-346.

Krisher, R.L.* (2004) The Impact of Oocyte Quality on Development. J. Anim. Sci. 82(E. Suppl.):E14-E23.

Durkin, R.E., Herrick, J.R., Bartels, P., Krisher, R.L. Preliminary observations on the development of in vitro embryo production systems for South African ungulates. Zoo Biol. Submitted.

Swain, J.E., Bormann, C.L., Krisher, R.L. Metabolism of glucose, pyruvate and glutamine by porcine oocytes matured in vitro and in vivo. Mol. Reprod. Dev. Submitted.

LOUISIANA

Behboodi, E., S.L. Ayres, E. Memili, B.C. Reggio, M. O'Coin, L. H. Chen, A. Landry, H. M. Meade, R.A. Godke and Y. Echelard. Health and reproductive profiles of transfected
somatic cell nuclear transfer derived goats producing malaria antigen. Cloning & Stem Cells (Submitted).

Collins II, M.G., J.W. Lynn, K. Bondioli and R.A. Godke. Co-culture of mammalian embryos with cells having increased HPRT activity. Molec. Reprod. Develop. (Under Review).

Devireddy,R.V., B. Fahrig, R.A. Godke and S.P. Leibo. 2004. The effect of extracellular ice and glycerol on boar sperm water transport parameters: convergence of theoretical and empirical rates of optimal freezing. Molec. Reprod. Develop. 67:446-457.

Godke, R.A., A. James, K. Hebert, R.S. Denniston, D. Paccamonti. 2004. The potential use of epididymal sperm from bulls and stallions for assisted fertilization. Havemeyer Fdn. Mono. 12:55-60.

Godke, R.A. and C. R. Youngs. 2004. Biotechnology: Embryo Technology for Cattle. W. Pond (ed). Marcel Dekker, Inc. New York, NY. (In press).

Gómez, M.C., C. E. Pope, A. G. Leslie, A. Lyons, R.F. Harris, A. L. King, A. Cole, R. A. Godke and B. L. Dresser. 2004. Birth of African Wildcat cloned kittens born from domestic cats. Cloning & Stem Cells 6:247-258.

Luster, S.M., M. Murakami, S.P. Leibo, R.S. Denniston Y. Echelard, and R.A. Godke. Pregnancies resulting from nuclear transfer embryos derived from vitrified bovine oocytes. Cryobiology (In press).

Murakami, M., T.R. Davidson, C.E. Ferguson, Y. Echelard and R. A. Godke. Use of the amniotic cavity of the developing chick embryo for culturing bovine 8-cell embryos derived from somatic cell nuclear transfer. Molec. Reprod. Develop. (In press).

Poleo, G.A., R. A. Godke and T.R. Tiersch. Intracytoplasmic sperm injection using cryopreserv-
ed, fixed and freeze-dried sperm in eggs of Nile tilapia. Marine Biotechnol. (In press).

Sansinena, M.J., B. Reggio, D. Hylan, A. Klumpp, D. Paccamonti, S.K. Lyle, R.S. Denniston, R.A. Godke. 2004. Effect of different pre-maturation treatments on in vitro maturation of equine oocytes and subsequent competence for nuclear transfer. Havemeyer Fdn. Mono. 12:23-25.

Sansinena, M., D. Hylan, K. Hebert, R.S. Denniston and R.A. Godke. Benteng (Bos Javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenology (In press).

Wirtu G, A. Cole, C.E. Pope, C.R. Short, R. A. Godke and B.L. Dresser. Handling eland antelope (Taurotragus oryx) for reproductive technology procedures without inducing general anesthesia: Roles of behavioral training and hydraulic chute. J. Zoo Wildlife Med. (In press).

Wirtu G, C.E. Pope, P. Damiani, B.L. Dresser, R.A. Godke and B.D. Bavister. 2004. Insulin improves frequency of cleavage and in vitro development of bovine embryos in defined medium. Reprod. Fertil. Develop. 16:1-11.

Wirtu G, C.E. Pope, J. Vaccaro, E. Sarrat, A. Cole, R.A. Godke and B.L. Dresser. 2004. Dominance hierarchy in a herd of female eland antelope (Taurotragus oryx) in captivity. Zoo Biol. 23:323-333.

MARYLAND

Lazaris, A., Keyston, R, and Keefer, C.L. (in press). Transgenesis using Nuclear Transfer in Goats. In: Methods In Molecular Biology, P. Verma and A. Trounson (eds),

Baldassarre, H., Wang, B., Pierson, J., Neveu, N., Sneek, L., Lapointe, J., Cote, F., Kafidi, N., Keefer, C.L., Lazaris, A., Karatzas, C.N. 2004. Prepubertal propagation of transgenic cloned goats by laparoscopic ovum pick-up and in vitro embryo production. Cloning & Stem Cells 6(1):25-29.

Keefer, C.L. 2004. Production of bioproducts through the use of transgenic animal models. Animal Reproduction Science 82-82:5-12.

USDA Beltsville

Blomberg, L.A. and K.A. Zuelke. 2004. Serial analysis of gene expression (SAGE) during porcine embryo development. Reprod Fert Dev 16:87-92.

Blomberg, L.A., E.L. Long, T.S. Sonstegard, C.P. Van Tassell, J.R. Dobrinsky, and K.A. Zuelke. 2004. Serial analysis of gene expression (SAGE) during elongation of peri-implantation porcine trophectoderm (conceptus). Physiological Genomics (published online November 9, 2004). DOI: 10.1152/physiogenomics.00157.2004.

Blomberg, L.A. and K.A. Zuelke. 2004. Expression analysis of the steroidogenic acute regulatory protein (STAR) gene in developing porcine conceptuses. Biol of Reprod (Submitted).

de A. Camargo, L.S., A.M. Powell, V.R. Do Vale Filho, and R.J. Wall. 2004. Comparison of gene expression in individual preimplantation bovine embryos produced by in vitro fertilization or somatic cell nuclear transfer. Reprod Fertil and Dev (Submitted).

Guthrie, H.D., R.J. Wall, V.G. Pursel, J.A. Foster-Frey, D.M. Donovan, G.R. Welch, and W.M. Garrett. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (BCL-2) transgene does not decrease atresia or increase ovulation rate in swine. Reprod Fertil and Dev (Submitted).

Guthrie, H.D. and G R. Welch. 2005. Impact of extender and cryopreservation on membrane and morphological integrity in boar sperm. Theriogenology 63: 396-410 (In Press).

Michael, D.D., O.M. Ocon, N.C. Talbot, and A.D. Ealy. 2004. Fibroblast growth factor-2 is expressed by the bovine uterus and stimulates interferon-tau production in bovine trophectoderm. Endocrinology (submitted).

Powell, A.M., N.C. Talbot, K.D. Wells, D.E. Kerr, V.G. Pursel, and R.J. Wall. 2004.
Cell Donor Influences Success of Producing Cattle by Somatic Cell Nuclear Transfer
Biol of Reprod 71:210-216.

Talbot, N.C., A.M. Powell, and T.J. Caperna. 2004. Colony-formation efficiency of bovine fetal fibroblast cell lines cultured with L-carnosine, hydrocortisone, low oxygen, or bFGF. Cloning and Stem Cells 6(1):35-45.
Talbot, N.C., M. Paape, W.M. Garrett, and T.J. Caperna. 2004. Macrophage population dynamics within fetal mouse fibroblast cultures derived from C57BL/6, CD-1, CF-1 mice and IL-6 and GM-CSF knock-out mice. In Vitro Cellular and Developmental Biology (In Press).
Talbot, N.C., T.J. Caperna, A.M. Powell, W.M. Garrett, and A.D. Ealy. 2004. Isolation and characterization of a bovine trophectoderm cell line derived from a parthenogenetic blastocyst. Molec Reprod and Dev 69(2):164-173.

Talbot, N.C., T.J. Caperna, A.M. Powell, A.D. Ealy, L.A. Blomberg, and W.M. Garrett. 2004. Isolation and characterization of a bovine visceral endoderm cell line derived from a parthenogenetic blastocyst. In Vitro Cell and Dev Biol  Animals (submitted).

Wall, R.J., A.M. Powell, M.J. Paape, D.E. Kerr, D.D. Bannerman, V.G. Pursel, K.D. Wells, N.C. Talbot, and H.W. Hawk. 2004. Genetically enhanced cows resist intramammary staphylococcus aureus infection. Nature Biotechnology (submitted).

UTAH

Li, G-P., K.L. White, K.I. Aston, L.N. Meerdo and T.D. Bunch. 2004. Condtioned medium increases the polyploidy cell composition of bovine somatic cell nuclear-transferred blastocysts. Reproduction. 127: 221-228.

Li, G-P. and T.D. Bunch. 2004. Modified air-dry methods reveals that diploid cells are predominant in cloned bovine embryos. Reprod. Fertil. Dev. 16:148.

Li, G-P., T.D. Bunch, K.L. White, K.I. Aston, L.N. Meerdo, B.J. Pate and B.R. Sessions. 2004. Development, chromosomal composition, and cell allocation of bovine cloned blastocyst derived from chemically assisted Enucleation and cultured in conditioned media. Mol. Reprod. Dev. 68:189-197.

Li, G-P., K.L. White and T.D. Bunch. 2004 Review of enucleation methods and procedures used in animal cloning: State of the art. Cloning and Stem Cells. 6:5-14.

Vanderwall, D.K., G.L. Woods, K.I. Aston, T.D. Bunch, G-P. Li, K.N. Meerdo and K.L. White. 2004. Cloned horse pregnancies produced using adult cumulus cells. Reprod. Fertil. Dev. 16:160.

WASHINGTON

Jiang, Z., X.L. Wu, M.D. Garcia, K.B. Griffin, J.J. Michal, M. Zhang, V.D. Wasem, T.L. Ott, C.T. Gaskins, and R.W. Wright, Jr. 2004. Comparative gene-based in silico transcriptome analysis of different tissues/organs in cattle. Genome 47:1164-1176.

WISCONSIN

Men, H. Y. Agca, J.J. Rutledge and J.K. Critser. 2004. Apoptosis during the development of oocytes and preimpoantation embryos. A.R.T. and Sci 4(2) 5-7.

Fischer-Brown, A, B. R. Lindsey, F. A. Ireland, D. L. Northey, R. L. Monson, S. G. Clark, M. B. Wheeler, D. J. Kesler, S. J. Lane, K. A. Weigel and J. J. Rutledge. 2004 Embryonic disc development and subsequent viability of cattle embryos following culture in two media under two oxygen concentrations. Reproduction, Fertility, and Development 16 (8); 787-793.


b. Books, Instructional Media, Non-Refereed Book Chapters, Proceedings from Scientific Meetings and Theses/Dissertations:

CALIFORNIA

McCarthy, M. 2004. The Effects of Reducing Endogenouse Estrogen in the Boar on Reproductive Tract Development, Sperm Production and Sperm Fertility

COLORADO

Coutinho da Silva, M.A., G.E. Seidel, Jr., E.L. Squires and E.M. Carnevale. 2004. Casein, native phosphocaseinate, and caseinoglycopeptide enhance binding of equine sperm to bovine zonae pellucidae. 6th Int. Equine Embryo Transfer Symposium, Brazil. In Press.

De La Torre-Sanchez, J.F. 2004. Regulation of Glucose Metabolism in Post-Compaction, In Vitro-Produced Bovine Embryos. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.

Walker, D.J. 2004. A Simple, Efficacious Method for Vitrification of Bovine Embryos. M.S. Thesis, Colorado State University, Fort Collins, CO.

ILLINOIS

Wheeler, M.B., Davidson, T.R., Malusky, S.A., Ferguson, C.E., Lima, A.S., Mello, M. B.
2004. PRODUCTION OF TRANSGENIC LIVESTOCK: Technology and Applications. In: Biotécnicas Aplicadas à Reprodução Animal, eds. P.B. Goncalves & J. Figuriedo, (new edition in press).

Wheeler, M.B., Malusky, S.A. 2004. Biotechnology: Stem Cell and Germ Cell Technology. Encyclopedia of Animal Science. Eds. W. G. Pond and A. W. Bell, pgs. 146-148.

Walters, E.M, Clark, S.G., Beebe, D. J., Wheeler, M.B. 2004. Mammalian Embryo Culture in a Microfluidic Device. In; Methods in Molecular Biology, vol. 254: Germ Cell Protocols, Volume 2 Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning, ed. H. Schatten, Humana Press Inc., Totowa, NJ, pgs. 375-382.

IOWA

Godke, R.A. and C.R. Youngs. 2004. Biotechnology: Embryo Technology for Cattle. In (W. Pond, Ed.) Encyclopedia of Animal Science (on-line). Marcel Dekker, New York, NY, DOI:10.1081/E-EAS 120019483, pp. 136-138.

LOUISIANA

Chapman, T.W. and Godke, R.A. 2004. Embryo collection and transfer in cattle. Proc. AVMA Central Veterinary Conference. pp. 812-815.

Davidson, T.R. 2004. The chick embryo amnion as an in vitro culture system for IVF and NT embryos. Ph.D. Dissertation. Louisiana State University. Baton Rouge.

Fahrig, B., C.E. Pope, R.A. Godke and S.P. Leibo. 2004. Effect of pellet volume on post-thaw survival of canine epididymal spermatozoa from individual dogs. Proc. Internatl. Congr. Anim. Reprod. 15:515.

Ferguson, C.E. 2004. The use of altrenogest to control reproductive function in beef cattle. Ph.D. Dissertation. Louisiana State University. Baton Rouge.

Gao, C., B. E. Eilts, Y. Han, J.A. Carter, R.S. Denniston and R.A. Godke. 2004. Using a nitrogen-pulse laser for assisted hatching of in vitro-cultured frozen-thawed bovine embryos. Proc. Internatl. Congr. Anim. Reprod. 15:420.

Godke, R.A. 2004. In vitro fertilization in farm animals. Proc. AVMA Central Veterinary Conference. pp. 816-818.

Godke, R.A. 2004. Transvaginal oocyte aspiration in farm animals. Proc. AVMA Central Veterinary Conference. pp. 819-822.

Godke, R.A. 2004. In vitro culture of farm animal embryos. Proc. AVMA Central Veterinary Conference. pp. 823-828.

Godke, R.A. 2004. Embryo microsurgery. Proc. AVMA Central Veterinary Conference. pp. 829-832.

Godke, R.A. 2004. Cloning and transgenic farm animals. Proc. AVMA Central Veterinary Conference pp. 833-836.

Godke, R.A. 2004. Transvaginal ultrasound-guided oocyte aspiration in farm animals. Proc. American/Canadian Embryo Transfer Association. (October 14-16th) Tampa, FL. pp. 81-90.

Godke , R.A. and K.R. Bondioli. Transvaginal oocyte aspiration and in vitro fertilization in beef cattle. Proc 4th World Italian Beef Cattle Congress. (In press).

James, A.N. 2004. Preservation of sperm harvested from the rat, caprine, equine and bovine epididymis. Ph.D. Dissertation. Louisiana State University. Baton Rouge.

Klumpp, A. 2004. The effect of holding bovine oocytes in follicular fluid on subsequent fertilization and embryonic development. M.S. Thesis. Louisiana State University. Baton Rouge.

Luster, S. 2004. Cryopreservation of bovine and caprine oocytes by vitrification. M.S. Thesis. Louisiana State University. Baton Rouge.

Wirtu, G. 2004. Ph.D. Application of bovine embryo technologies to the eland antelope. (Taurotragus oryx). Dissertation. Louisiana State University. Baton Rouge.

USDA

Guthrie, H.D. 2004. The follicular phase in Pigs: Follicle populations, circulating hormones, follicle factors and oocytes. Invited paper at the Second Billy N. Day Symposium in Reproductive Biology The Follicular Phase in Pigs: Physiology and Potential for Regulation to Benefit Pork Production at the Annual Midwestern section Meeting of the American Society of Animal Science, Des Moines, IA, March 16.

Guthrie, H.D. 2004. What's current and needed for freezing and sexing technology? Invited presentation at NCR-57 Boar Physiology Workshop Ames, IA, May 26.

Zuelke K.A. and L.A. Blomberg. 2004. Serial analysis of gene expression (SAGE) during porcine embryo elongation. IETS Newsletter 22(3):10-14.

WISCONSIN

Fischer-Brown, Amy. 2004. Culture System-specific Effects on Development of Invitro-produced Bovine Embryos. Ph.D. Dissertation, University of Wisconsin Library, Madison, WI 53705

Rutledge, J.J. 2004. Technology Innovations to enhance livestock agribusiness. Proc Conf: Seminar Nasional Teknologi Perternakan dan Veteriner, Bogor, Indonesia

Jensen, N.L. 2004. Bovine In Vitro Embryo Production Using Avian White
Yolk. M.S. Thesis, University of Wisconsin Library, Madison, WI 53705


c. Abstracts:

ARKANSAS

Kreider, D., N. Post, R. Rorie, T. Lester and E. French. 2004. MGA and CIDR based timed AI protocols in postpartum beef cows. J. Anim. Sci. 82(Suppl. 1):255.

Post, N.M., D.L. Kreider, R.W. Rorie and T.D. Lester. 2004. Effects of supplemental progesterone in a timed insemination protocol in beef heifers. Proc. So. Sec., ASAS (in press).

Rorie, R.W., C.F. Rosenkrans and A.J. Aishman. 2004. Effects of bovine somatotropin treatment on the AI pregnancy rate in dairy heifers. Reprod. Fertil. Develop. 16:132.

CALIFORNIA

At-Taras, E.E., I.C. Kim, T. Berger, and J.F. Roser. 2004 Effects of in vivo aromatase inhibition on Leydig cell function. Biol. Reprod. (Special Issue): 259.

McCarthy, M., C. Pearl, T. Berger, and J.F. Roser. 2004 Androgen and estrogen regulation of the boar epididymis: concentrations and receptor localization. Biol. Reprod. (Special Issue): 133.

Ducummon, C.C. and T. Berger. 2004 The monomeric G-protein RhoA is widely expressed in mammalian sperm, but does not play a role in actin cytoskeletal reorganization in porcine sperm. Biol. Reprod. (Special Issue): 185

COLORADO

Barcelo-Fimbres, M. and G.E. Seidel, Jr. 2004. Optimizing sperm concentration to maximize monospermy and minimize polyspermy with bovine in vitro fertilization. J. Anim. Sci. 82 (Suppl. 1):371.

Carnevale, E.M., W.D. Eldridge-Panuska, V. Caracciolo di Brienza, G.E. Seidel, Jr. and E.L. Squires. 2004. Embryo development rates after vitrification and transfer of equine embryos. Proc. 6th Int. Symp. Equine Embryo Transfer, Rio de Janeiro, p. 20.

Coutinho da Silva, M.A., G.E. Seidel, Jr., E.L. Squires and E.M. Carnevale. 2004. Casein, native phosphocaseinate and caseinoglycopeptide enhance binding of equine sperm to bovine zonae pellucidae. Proc. 6th Int. Symp. Equine Embryo Transfer, Rio de Janeiro, p. 24.

Coutinho da Silva, M.A., G.E. Seidel, Jr., E.L. Squires, Y.H. Choi and E.M. Carnevale. 2004. Binding of stallion sperm to equine and bovine zonae pellucidae: Effect of milk proteins and glucose. Reprod. Fert. Dev. 16:252 (abstr. #263).

Coutinho da Silva, M.A., J.E. Stokes, G.E. Seidel, Jr., E.L. Squires and E.M. Carnevale. 2004. Fertilization of bovine oocytes using sperm selected by adhesion to oviduct epithelial cells in vitro. Proc. 15th Int. Congress Animal Reproduction, Brazil, Abstracts Vol. 2, p. 414.

De La Torre Sanchez, J.F., M. Lane, J. Gibbons, D.K. Gardner and G.E. Seidel, Jr. 2004. Glucose metabolism of in vitro and in vivo produced bovine embryos. Reprod. Fert. Dev. 16:196 (abstr. #149).

Horvath, G. and G.E. Seidel, Jr. 2004. Macromolecules for vitrifying bovine oocytes. Reprod. Fert. Dev. 16:171 (abstr. #99).

Souza, A.H., R. Sartori, J.N. Guenther, D.Z. Caraviello, G.E. Seidel, Jr., J.L. Schenk and M.C. Wiltbank. 2004. Fertilization rate and embryo quality in superovulated Holstein heifers artificially inseminated with X-sorted or unsorted sperm. Proc. 15th Int. Congress Animal Reproduction, Brazil, Abstracts Vol. 2, p. 358.

Stokes, J.E., E.M. Carnevale, E.L. Squires and G.E. Seidel, Jr. 2004. Preparation of bovine and equine sperm by Percoll or swim up for injection into bovine oocytes. Proc. 15th Int. Congress Animal Reproduction, Brazil, Abstracts Vol. 2, p. 471.

Walker, D.F., L.F. Campos-Chillon and G.E. Seidel, Jr. 2004. Vitrification of in vitro produced bovine blastocysts by addition of cryoprotectant in one step. Reprod. Fert. Dev. 16:184 (abstr. #123).

ILLINOIS

Lopes, P.H.C., Malusky, S.A., Lima, A.S., Beebe, D.J., Wheeler, M.B. (2004) Mouse embryonic development from one-cell stage to blastocyst utilizing KSOM supplemented with amino acids in a microchannel device. Reproduction, Fertility and Development 16:205, abstr. 166.

Marshall, K.M., Shanks, R.D., Hurley, W.L., Donovan, S.M., Wheeler, M.B. (2004) Effect of increased suckling stimulation on piglet weight gain and daily milk yield of sows transgenic for bovine a-lactalbumin. Reproduction, Fertility and Development 16:291, abstr. 342.

Clark, S.G., Walters, E.M., Beebe, D. J., Wheeler, M.B. (2004) In vitro fertilization in microfluidic channels enhances monospermic penetration of swine oocytes. Reproduction, Fertility and Development 16:251, abstr. 262.

Malusky, S.A. Wheeler, M.B. Isolation of Adult Adipose Derived Stem Cells from Transgenic Swine. International Society for Stem Cell Research (ISSCR), 2nd Annual Meeting, Abstract 248 page 124.

INDIANA

Brad, A.M., Spies, T., Olivier, F., Lane, M., Gardner, D.K., Bartels, P., Krisher, R.L. 2004. Timing of In Vitro Oocyte Maturation in Springbok (Antidorcas marsupialis), Black Wildebeest (Connechaetes gnu), Blesbok (Damaliscus dorcus phillipsi), and Reedbuck (Redunca arundinum). Reprod. Fertil. Dev. 16:223 (abstr. 203).

Conover-Sparman, M.L., Krisher, R.L. 2004. Ion composition of culture medium influences mitochondrial distribution and blastocyst development of preimplantation porcine embryos. Reprod. Fertil. Dev. 16:195 (abstr. 147).

Herrick, J.R., Behboodi, E., Memili, E., Blash, S., Echelard, Y., Krisher, R.L. 2004. Effect of macromolecule supplementation during in vitro maturation on the developmental competence of goat oocytes. Reprod. Fertil. Dev. 16:276 (abstr. 314).

Herrick, J.R., Bond, J.B., Bateman, H.L., Manharth, A.L., Krisher, R.L., Swanson, W.F. 2004. Comparative Energy Metabolism and Development of In Vivo- and IVF-Derived Domestic Cat Embryos. Biol. Reprod. Special issue:214 (abstr. 532).

Krisher, R.L. 2005. Inhibition of the pentose phosphate pathway results in meiotic arrest in porcine oocytes that can be overcome by the addition of pathway cofactors and end products. Reprod. Fertil. Dev. 17:293 (abstr. 287).

Lee, K., Fodor, W.L., Machaty, Z. 2005. The presence of lamin A/C antigens in porcine embryos. Reprod. Fertil. Dev. 17:206 - 207 (abstr. 112).

Swanson, W.F., Manharth, A.L., Bond, J.B., Bateman, H.L., Krisher, R.L., Herrick, J.R. 2004. Effects of extracellular ion concentrations on in vitro development of domestic cat IVF embryos. Reprod. Fertil. Dev. 16:202 (abstr. 160).

Tubman, L., Peter, A., Krisher, R.L. 2005. Effect of energy substrates on metabolism and meiosis of porcine oocytes during in vitro maturation. Reprod. Fertil. Dev. 17:301 (abstr. 301).

IOWA

Pugh, M.L., M.B. Moreira, G.R. Gilbert and C.R. Youngs. 2004. Influence of prostaglandin F2a synthesis inhibitors on pregnancy rate of embryo transfer recipient heifers. Proc. 15th Intl. Cong. Anim. Reprod. 2:399.

Youngs, C.R., A.M. Powers-Meyer, M.G. Wonderlich and M.B. Moreira. 2004. The effect of cloprostrenol or dinoprost, administered at two different sites, on the expression of estrus in postpartum dairy cattle. Proc. 15th Intl. Cong. Anim. Reprod. 1:60.

LOUISIANA

Aljarrah A. H., D.L. Paccamonti, B.E. Eilts, S.K. Lyle, D. Hylan, M.S. Ferrer and R.A. Godke. 2004. Follicular aspiration or deslorelin treatment to initiate cyclicity in transitional mares. Soc. Theriogenol. (Abstr).

Davidson, T.R., C.E. Ferguson, J. Lynn, T. Chapman, K. Hebert, M.B. Wheeler, K. Bondioli and R.A. Godke. Evaluation of apoptosis in bovine embryos by fluorescent labeling of caspases-3 and -7. Reprod. Fertil. Develop. 17: (In press).

Ferguson, C.E., D.J. Kesler, T.R. Davidson, B. Lenard and R.A. Godke. 2004. Saving pregnancies in beef cattle after a luteolytic dose of prostaglandin. Reprod. Fertil. Develop. 16:209.

Ferguson , C.E., T.R. Davidson, M.R.B. Mello, A.S. Lima, D.J. Kesler, M.B. Wheeler and
R.A. Godke. Evidence of a direct effect of P4 on IVF-derived Bovine 8-cell embryos. Reprod.
Fertil. Develop. 17: (In press).

Giraldo, A.M., J. W. Lynn, E.C. Pope, R. A. Godke and K. R. Bondioli. Lifespan and chromosomal stability of bovine and porcine fetal fibroblast cells cultured in vitro. Reprod. Fertil. Develop. 17: (In press).

Gómez, M.C., C.E. Pope, A.M. Giraldo, L. Lyons, R.F. Harris, A. King, A. Cole, R.A. Godke and B.L. Dresser. 2004. Birth of African Wildcat cloned kittens. Reprod. Fertil. Develop.16:141.

Klumpp, A. M., R.S. Denniston D. Paccamonti, S.P. Leibo, and R.A. Godke. 2004. Embryonic development after holding bovine oocytes in undiluted follicular fluid for a 6-hour prematuration period. Reprod. Fertil. Develop.16:278.

Landry, A.M., H. DiMaggio, W. Burnside, L. Sarradet, J. Saenz, D.J. Landry, L.R. Gentry,
K. Bondioli and R.A. Godke. The effect of hCG on circulating progesterone in goats at the
end of the breeding season. Southern Section, Amer. Soc. Anim. Sci. (In press).

Landry,A.M., M. Murakami, R.S. Denniston, J.L. Williams, Y. Echelard, and R.A. Godke
Development of bovine aggregate embryos constructed from nuclear transfer embryos and electrofused IVF-derived embryos. Reprod. Fertil. Develop.17: (In press).

Moisan, A.E., E.L.Chamberlain, S.P. Leibo, B.L. Dresser, K. Bondioli and R.A. Godke. Blastocyst formation from vitrified bovine oocytes, zygotes and two-cell embryos. Reprod. Fertil. Develop. 17: (In press).

Moisan, A.E., J.W. Lynn, S.P. Leibo, R.S. Denniston and R.A. Godke. 2004. Volumetric changes of bovine oocytes resulting from dehydration in disaccharides solution. Reprod. Fertil. Develop.16:177.

Nel, L., R.A. Godke, B.L. Dresser and P. Damiani. 2004. Isolation, culture and cryopreserva- tion of somatic cells obtained from fresh and cooled semen, and fresh milk of gulf coast native sheep. Reprod. Fertil. Develop.16:152.

Nel, L., P. Damiani, B.L. Dresser and R.A. Godke. 2004. Freezing animal skin biopsies
under field conditions. Proc. AZA Conference. (Abstr).

Nel-Themaat, L., M.C. Gomez, A. Cole, G. Wirtu, K.R. Bondioli, B.L. Dresser R.A. Godke and C.E. Pope. Somatic cell isolation from semen by percoll gradients. Reprod. Fertil. Develop. 17: (In press).

Roberts, J.L., R.P. DelVecchio, G.T. Gentry, Jr., D. Sanders, P.E. Humes and R.A. Godke. Effect of melengestrol acetate (MGA) and monensin supplementation on puberty and pregnancy rates in crossbred beef heifers. Southern Section, Amer Soc Anim Sci. (In press).

Sansinena, M., J. Lynn, R. Denniston and R. Godke. Ooplasmic transfer after interspecies
nuclear transfer: Presence of foreign mitochondria, pattern of migration, and effect on
embryo development. Reprod. Fertil. Develop. 17: (In press)

Wirtu, W., C. E. Pope, P. Damiani, B.L. Dresser, R.A. Godke and B.D. Bavister. 2004. Insulin improves frequency of cleavage and subsequent development of bovine embryos in vitro. Reprod. Fertil. Develop.16:203.

Wirtu, G. C. E. Pope, A. Cole, R. A. Godke and B. L. Dresser. 2004. Behavioral training
and handling of adult female eland antelope (Taurotragus oryx) in a hydraulic chute. Proc AZA Conference. (Abstr.)

MARYLAND

He, S., Pant, D., Bischoof, S., Melican, D., Gavin, W., Keefer, C.L. (2004). Expression of embryonic stem cell (ESC) markers in preimplantation goat embryos. Biol. Reprod. (S1):212, abst. 522.

He, S., Pant, D., Bischoff, S., Gavin, W., Melican, D., Keefer, C.L., (2005). Expression of pluripotency-determining factors Oct-4 and Nanog in preimplantation goat embryos. Reprod. Fert. Dev.: in press.

USDA Beltsville

Blomberg, L.A., J.R. Dobrinsky, and K.A. Zuelke. 2004. Steroidogenic acute regulatory protein (STAR) gene expression coincides with increased estrogen synthesis in porcine embryos. Society for the Study of Reproduction, Vancouver, British Columbia, Canada, August 3.

Donovan, D.M., R.J. Wall, D. Pritchard, and N.C. Talbot. 2004. Expression of a group B streptococcus bacteriophage lysine in mammalian cells. American Society for Microbiology annual meeting.

Guthrie, H.D., R.J. Wall, V.G. Pursel, J.A. Foster-Frey, D.M. Donovan, G.R.Welch, and W.M. Garrett. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (BCL-2) transgene does not decrease atresia or increase ovulation rate in swine. Society for the Study of Reproduction annual meeting. Vancouver, British Columbia, Canada, August 2.

Mitchell, A.D. and R.J. Wall. 2004. In vivo evaluation of changes in body composition of transgenic mice expressing the myostatin pro domain using dual energy X-ray absorptiometry. Society of Experimental Biology annual meeting.

Powell, A.M., N.C. Talbot, K.D. Wells, D.E. Kerr, V.G. Pursel, and R.J. Wall. 2004. Effect of roscovitine on fibroblasts ability to form blastocysts and establish pregnancies after bovine nuclear transfer. Annual Meeting of the International Embryo Transfer Society, January 10-14; Reproduction, Fertility, and Development 16: 155.

Pursel, V.G., A.D. Mitchell, and R.J. Wall. 2004. Progress on using transgenes to produce lean pork. In: Present and Future of Transgenic cloned pigs. 2nd International Symposium of Research for Transgenic Cloned Pigs. Daejeon, Korea.

Rodriguez, K.F., L.A. Blomberg, K.A. Zuelke, J. Miles, J. Alexander, and C. Farin. 2004. Use of serial analysis of gene expression (SAGE) for identification of candidate genes associated with gonadotropin-induced oocyte maturation in the mouse. Society for the Study of Reproduction, Vancouver, British Columbia, Canada, August 2.

Talbot, N.C., O.M. Ocon, A.M. Powell, and A.D. Ealy. 2004. Interferon-tau expression from the trophectoderm outgrowths of primary bovine blastocyst culture: comparison between IVP, NT, and parthenogenote embryos. Annual Meeting of the International Embryo Transfer Society, January 10-14; Reproduction, Fertility, and Development 16: 153.

Talbot, N.C. and A.M. Powell. 2004. Effect of bovine pituitary extract on hatching and post-hatching development of IVP bovine embryos. Annual meeting of the International Embryo Transfer Society, January 10-14; Reproduction, Fertility, and Development 16: 202.

Zhao, B., R.J. Wall, and J. Yang. 2004. Prevention of high-fat diet induced insulin resistance by transgenic interruption of myostatin function in mice. North American Association for the Study of Obesity annual meeting.

UTAH

Aston, K.I, G-P. Li, B. Hicks, B.R. Sessions, B..J. Pate, L.N. Meerdo, D.S. Hammon, T.D. Bunch and K.L. White. 2004. The developmental competence of bovine nuclear transfer (NT) embryos derived from cow verses heifer ooplasts. SSR.

Bunch, T.D. and G-P. Li. 2004. Conditioned media effects chromosome composition of bovine cloned emblryos. Proc. Pac Div. AAAS.


Pate, B.J., G-P. Li, K.I. Aston, T.D. Bunch, K.D. Campbell, D.S. Hammon, L.F. Rickords and K.L. White. 2004. Specific integrins subunits present on the vitelline membrance of bovine oocytes. SSR.

WISCONSIN

Fischer-Brown, A., R. Monson,D. Northey, T. Kuhlka and J. Rutledge. 2004. Pregnancy and parturition following transfer of bovine embryos cultured in two media under two oxygen concentrations. Reprod, Fert and Devel:16(1,2):197.

Nguyen, B.X., N.T. Uoc, L.V. Ty, R.L. Monson, M.L. Leibfried-Rutledge, N.H.duc, L.C. Bui, Q.X. Huu, N.V. Hanh, N.T. Tanh, N.V. Linh and J.J. Rutledge. 2004. Production of tropical dairy calves by embryo transfer using local Laisind (Bos incicus) recipients and intercontinental fresh IVF shipped embryos. Reprod, Fert and Devel:16(1,2):249-250.

Wilson, R.D., K.A. Weigel, P.M. Fricke, M.L. Leibfried-Rutledge, D.L. Matthews, V.R. Schutzkus, and J.J. Rutledge. 2004. System for in vitro production with sexed sperm in commercial dairy herds. J. Dairy Sci: Vol. 87 (suppl.1): 258.

Wilson, R.D., K.A. Weigel, P.M. Fricke, M.L. Leibfried-Rutledge, D.L. Matthews, and J.J. Rutledge. 2004. In vitro production of Holstein embryos using Beltsville method sex-sorted sperm. J. Dairy Sci: Vol 87 (suppl. 1): 366.

Wilson, R.D., K.A. Weigel, P.M. Fricke, M.L. Leibfried-Rutledge, d.L. Matthews, J.J. Rutledge, and V.R. Schutzkus. 2004. In vitro production of Holstein embryos using sex sorted semen. Reprod, Fert and Devel:16(1,2):263.


d. Miscellaneous Publications (Semi-Technical/Lay Publications):

CONNECTICUT
We transferred two technologies to a start-up company spun off from the University of Connecticut: 1) NT with long-term cultured cells for gene knockout; and 2) embryo/oocyte droplet vitrification technology. We also filed disclosure on a novel cloning technology in the pig.

LOUISIANA

Roberts, J.L., R.P. DelVecchio, G.T. Gentry, Jr., D. Sanders, P.E. Humes and R.A. Godke. Effect of melengestrol acetate and monensin supplementation on pregnancy rates in crossbred beef heifers. Louisiana Beef Cattle Rep. (In press).

USDA Beltsville

Smith 3rd, J.E., W.D. Rees, M.P. Richards, and R.J. Wall. 2004. Codon-optimization of threonine biosynthetic genes for mammalian cells. Beltsville Area Research Center Poster Day. March 24. 2004.

WISCONSIN

Fischer-Brown A. and J. Rutledge. 2004. Practical and research applications of elongation stage bovine embryos. IETS newsletter 21(3):11-15.

Rutledge, J.J.2004. Technology Innovations to Enhance Livestock Agribusiness. Babcock Dairy Update, Babcock Institute of International Dairy Research and Development, 204 Agriculture Hall, College of Agricultural and Life Sciences, University of Wisconsin, Madison, WI 53706

05/29/2006

REFEREED ARTICLES/BOOK CHAPTERS:

ARKANSAS:

Burke, J.M., C.F. Rosenkrans, R.W. Rorie, C. Golden and J.K. Apple. 2006. Reproductive responses of ram lambs under short-term exposure to endophyte-infected tall fescue seed. Small Rum Res (in press).

Post, N.M., D.L. Kreider and R.W. Rorie. 2005. Timed insemination in beef heifers after synchronization of estrus with controlled intravaginal drug releasing (CIDR) device and mesengesterol acetate (MGA). Prof. Anim. Sci 21:107-113.

Post, N.M., D.L. Kreider, R.W. Rorie and T.D. Lester. 2005. Timed AI in postpartum beef cows using melengestrol acetate, PGF2alpha and GnRH. Prof. Anim. Sci 21:17-21.


CALIFORNIA:

At-Taras, E.E., A.J. Conley, T. Berger, and J.F. Roser. 2006. Reducing estrogen synthesis does not affect gonadotropin secretion in the developing boar. Biology Reproduction(in press).

Batchelder, C.A., K. Hoffert, M. Bertolini, A.L. Moyer, J.B. Mason, S.G. Petkov, T.R. Famula and G.B. Anderson. 2006. Effect of the nuclear-donor cell lineage, type, and cell donor on development of somatic cell nuclear transfer embryos in cattle. Cloning and Stem Cells (in press).

Hoffert, K.A, C.A. Batchelder, M. Bertolini, A.L. Moyer, T.R. Famula, D.L. Anderson, and G.B. Anderson. 2006. Measures of maternal-fetal interaction in day-30 bovine pregnancies derived from nuclear transfer. Cloning and Stem Cells (in press).

Khalil,M.B.; K. Chakrabandhu, H. Xu, W. Weerachatyanukul, M. Buhr, T. Berger, E. Carmona, N. Vuong, P. Kumarathasan, , P. T.T. Wong, D. Carrier, and N. Tanphaichitr. 2006. Sperm capacitation induces an increase in lipid rafts having zona pellucida binding ability and containing sulfogalactosylglycerolipid. Developmental Biology (in press).

COLORADO:

Eldridge-Panuska, W.D., V. Caracciolo di Brienza, G.E. Seidel, Jr., E.L. Squires and E.M. Carnevale. 2005. Establishment of pregnancies after serial dilution or direct transfer by vitrified equine embryos. Theriogenology 63:1308-1319.

Lindsey, A.C., D.D. Varner, G.E. Seidel, Jr., J.E. Bruemmer and E.L. Squires. 2005. Hysteroscopic or rectally guided, deep-uterine insemination of mares with spermatozoa stored at 18 h at either 5°C or 15°C prior to flow-cytometric sorting. Anim. Reprod. Sci. 85:125-130.

Preis, K.A., G.E. Seidel, Jr. and D.K. Gardner. 2005. Metabolic markers of developmental competence for in vitro-matured mouse oocytes. Reproduction 130:475-483.

CONNECTICUT:

Chang C.C., Y.H. Ma, S. Jacobs, X.C. Tian, X. Yang, and T.Rasmussen. 2005. Histone macroH2A1 expression dynamics during material to embryonic transition. Dev Biol 278:367-380.

Chaubal S.A., J.A. Molina, C.A. Ohlrichs, L.B. Ferre, D.C. Faber, P.E.J. Bols, J. Riesen, X.C. Tian, and X.Yang. 2006. Different transvaginal ovum pick-up techniques in cows to optimize oocyte retrieval and embryo production over a fixed period of time. Theriogenology (in press).

Curchoe, C., S.Q. Zhang, L. Yang, Y.F. Bin, X.Q. Zhang, D.Y. Feng, and X.C. Tian. 2005. Promoter-specific expression of the imprinted IGF2 gene in cattle. Biol Reprod 73:1275-1281.

Du, F., P. Shen, J. Xu, L. Sung, B.-S. Jeong, J. Riesen, W.T.K. Cheng, S.-N. Lee, X. Yang, and X.C. Tian. 2006. Phytohemagglutinin-cell agglutination agent and embryo vitrification improves the efficiency of somatic cloning in cattle (Bos Taurus). Theriogenology (in press).

Enright. B.P., L.Y. Sung, C.C. Chang, X. Yang, and X.C. Tian. 2005. Methylation and acetylation characteristics of cloned bovine embryos from donor cells treated with 5-aza-2'-deoxycytidine. Biol Reprod 72:944-948.

Nedambale T.L., X. Yang, and X.C. Tian. 2006. Effects of culture media on the development of in vitro-derived bovine embryos, and of 2-mercaptoethanol on post-vitrification survival. Anim Reprod Sci (in press).

Smith, S.L., R.E. Everts, X.C. Tian, F. Du, L.-Y. Sung, S. Rodrigues-Zas, B.-S. Jeong, J.P. Renard, H.A. Lewin, and X. Yang. 2005. Global gene expression profiles of cloned blastocysts reveal significant nuclear reprogramming. Proc. Natl. Acad. Sci, USA 102:17582-17587.

Sung, L.-Y., F. Du, J. Xu, W. Chang, T.L Nedambale, J. Zhang. S. Jiang, X.C. Tian, and X. Yang. 2005. The effect of albumin and sodium citrate on the development of in vitro produced bovine embryos under different oxygen tension. Reprod Nutr Dev 44:551-64.

Suteevun, T., R. Parnpai, S.L. Smith, C.-C. Chang, S. Muenthaisong, and X.C. Tian. 2006. Epigenetic characteristics of cloned and in vitro fertilized swamp buffalo (Bubalus bubalis) embryos. J Anim Sci (in press)

Suteevan, T., S. Smith, S. Muentaisong, X. Yang, R. Parnpai, and X.C. Tian. 2006. Temporal expression of chromatin remodeling genes in single swamp buffalo (Bubalus bubalis) cloned and in vitro fertilized embryos. Theriogenology (in press).

Tian, X.C., C. Kubota, K. Sakashita, Y. Izaike, R. Okano, N. Tabara, C. Curchoe, L. Jacob, Y. Zhang, S. Smith, C. Bormann, S. Andrew, and X. Yang. 2005. Meat and milk compositions of bovine clones compared with matched controls. Proc Natl Acad Sci USA 102: 6261-6266.

Vasques, L.R., R. Stabellini, F. Xue, X.C. Tian, M. Soukoyan, and L.V. Pereira. 2006. Xist repression in the absence of Dnmt1 and Dnmt3B. J DNA Research (in press).

Wang, L., E. Duan, L.-Y. Sung, X. Yang, and X.C. Tian. 2005. Generation and characterization of pluripotent putative stem cells from cloned bovine embryos. Biol Reprod 73:149-155.

Yang, L., P. Chavatte-Palmer, C. Kubota, M. O'Neill, M. Taneja, T. Hoagland, J.-P. Renard, X. Yang X, and X.C. Tian. 2005. Expression of imprinted genes is aberrant in deceased newborn cloned calves and relatively normal in surviving adult clones. Mol Reprod Dev 71:431-438.

ILLINOIS:

Clark, S.G., D.J. Beebe, and M.B. Wheeler. 2005. Reduction of polyspermic penetration using novel microfluidic technology during in vitro fertilization. Lab on a Chip 5:1229-1232.

Hartke, J.L., M.H. Monaco, M.B. Wheeler, and S.M. Donovan. 2005. Effect of a short-term fast on intestinal disaccharidase activity and villus morphology in piglets suckling insulin-like growth factor-I (IGF-I) transgenic sows. J. Animal Sci. 83:2404-2413.

Monaco, M.H., D.E. Gronlund, G.T. Bleck, W.L. Hurley, M.B. Wheeler, and S.M. Donovan. 2005. Mammary specific transgenic over-expression of insulin-like growth factor-I (IGF-I) increases pig milk IGF-I and IGF binding proteins, with no effect on milk composition or yield. Transgenic Research 14:761-777.

Reeder, A.L., M.O. Ruiz, A. Pessier, L.E. Brown, J.M. Levengood, C.A. Phillips, M.B. Wheeler, R.E. Warner, and V.R. Beasley. 2005. Intersexuality and the cricket frog decline: Historic and geographic trends. Environ Health Perspect 113:261-265.

Zeringue, H.C., M.B. Wheeler, and D.J. Beebe. 2005. A microfluidic method for removal of the zona pellucida from mammalian embryos. Lab on a Chip, 5:108-110.


INDIANA:

Herrick, J.R., E. Behboodi, E. Memili, S. Blash, Y. Echelard, and R.L. Krisher. 2006. Metabolism, protein content and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate. Molecular Reproduction and Development 73:256-266. (Published Online: 25 Oct 2005.)

Herrick, J.R., A.M. Brad, and R.L. Krisher. 2006. Chemical manipulation of glucose metabolism in porcine oocytes: Effects on nuclear and cytoplasmic maturation in vitro. Reproduction (in press).

IOWA:

Godke, R.A. and C. R. Youngs. 2005. Biotechnology: Embryo Technology for Cattle. W. Pond (ed). Marcel Dekker, Inc. New York, NY. pp.136-138. (reviewed book chapter)

Youngs, C.R. and R.A. Godke. 2006. Biotechnology: Cattle Embryo Transfer Technology. In (D.R. Heldman, A. Bridges, D. Hoover, and M.B. Wheeler, Eds.) Encyclopedia of Biotechnology in Agriculture and Food. Marcel Dekker, New York, NY (in press).

LOUISIANA:

Behboodi, E., S. L. Ayres, E. Memili, B.C. Reggio, M. O'Coin, L. H. Chen, A. Landry, H. M. Meade, R. A. Godke and Y. Echelard. 2005. Health and reproductive profiles of transfected somatic cell nuclear transfer derived goats producing malaria antigen. Cloning & Stem Cells 7(2):107-108.

Godke, R.A. and C. R. Youngs. 2005. Biotechnology: Embryo Technology for Cattle. W. Pond (ed). Marcel Dekker, Inc. New York, NY. pp.136-138. (reviewed book chapter)

Landry, A.M., D.J. Landry, L.R. Gentry, H.L. Green, B. Reggio, K.L. Koonce, Y. Echlard and R.A. Godke. 2005. Endocrine profiles and growth patterns of goats produced by nuclear transfer. Cloning & Stem Cells 7(4):214-225.

Luster, S.M., M. Murakami, S.P. Leibo, R.S. Denniston Y. Echelard, and R.A. Godke. 2006. Pregnancies resulting from nuclear transfer embryos derived from vitrified bovine oocytes. Cryobiology (in press).

Murakami, M., T.R. Davidson, C.E. Ferguson, Y. Echelard and R. A. Godke. 2006. Use of the amniotic cavity of the developing chick embryo for culturing bovine 8-cell embryos derived from somatic cell nuclear transfer. Molec. Reprod. Develop. (in press).

Murakami, M., C.E. Ferguson, O. Perez, A. Boediono, D. Paccamonti, K. Bondioli and R. A. Godke. 2006. Transfer of inner cell mass cells derived from bovine nuclear transfer embryos into the trophoblast of bovine in vitro-produced embryos. Cloning & Stem Cells (in press).

Nel-Themaat, L., G.D. Harding, J.E. Chandler, J.F. Chenevert, P. Damiani, J.M. Fernandez, P.E. Humes, C.E. Pope and R.A. Godke. 2006. Quality and freezability of first and second ejaculates collected from endangered Gulf Coast native rams. Anim. Reprod. Sci. (in press).

Poleo, G.A., R. A. Godke and T.R. Tiersch. 2005. Intracytoplasmic sperm injection using cryopreserved, fixed and freeze-dried sperm in eggs of Nile tilapia. Marine Biotechnol. 7(2): 104-111.

Sansinena, M., D. Hylan, K. Hebert, R.S. Denniston and R.A. Godke. 2005. Benteng (Bos Javanicus) embryos and pregnancies produced by interspecies nuclear transfer. Theriogenology 63(4):1081-1091.

Wirtu G, A. Cole, C.E. Pope, C.R. Short, R. A. Godke and B.L Dresser. 2005. Handling eland antelope (Taurotragus oryx) for reproductive technology procedures without inducing general anesthesia: Roles of behavioral training and hydraulic chute. J. Zoo Wildlife Med. 36(1):1-11.


MARYLAND:

Betts, D.H., Perault, S.D., Petrik, J., Lin, L., Favetta, L.A., Keefer, C.L., King, W.A. (2005). Telomere length analysis in goat clones and their offspring. Molecular Reproduction and Development 72(4):461-470.

Lazaris, A., R. Keyston, and C.L. Keefer. 2006. Transgenesis using nuclear transfer in goats. In: (P. Verma and A. Trounson , Eds.) Methods In Molecular Biology (in press).


NORTH CAROLINA:

Farin, P.W., J.A. Piedrahita, and C.E. Farin. 2006. Errors in development of fetuses and placentas from in vitro-produced bovine embryos. Theriogenology (in press).

Miles J.R., C.E. Farin, K.F. Rodriguez, J.E. Alexander, and P.W. Farin. 2005. Effects of embryo culture on angiogenesis and morphometry of bovine placentas during early gestation. Biol Reprod 73:663-671.

Mir, B., G. Zaunbrecher, G.S. Archer, T. Friend, and J.A. Piedrahita. 2005. Progeny of somatic cell nuclear transfer (SCNT) clones are phenotypically similar to non-cloned pigs. Cloning and Stem Cells 7:119-125.


OREGON:

Singleton, C. and A.R. Menino, Jr. 2005. Effects of inhibitors of integrin binding on cellular outgrowth from the bovine inner cell mass. In Vitro Cell. Dev. Biol.-Animal 40:29-37.


UTAH:

Li, G-P., Y. Liu, T.D. Bunch, K.L. White, and K.I. Aston. 2005. Asymmetric division of spindle microtubules and microfilaments during bovine meiosis from metaphase I to metaphase III. Mol. Reprod. Dev. 71:220-226.

Li, G-P., Y. Liu, K.L. White, and T.D. Bunch. 2005. Cytogentic analysis of diploidy in cloned bovine embryos using an improved air-dry karyotyping method. Theriogenology. 63:2434-2444.

Meerdo, L.N., Reed, W.A., and K.L. White. 2005. Telomere to centromere ratio of bovine clones, embryos, gametes, fetal cells, and adult cells. Cloning and Stem Cells. 7:61-72.

Wang, S., K.E. Panter, W. Gaffield, R.C. Evans and T.D. Bunch. 2005. Effects of steroidal glycoalkaloids from potatoes (Solanum tuberosum) on in vitro bovine embryo development. Anim. Reprod. Sci. 85:243-250.


USDA-ARS (Beltsville):

Blomberg, L.A., and K.A. Zuelke. 2005. Expression analysis of the steroidogenic acute regulatory protein (STAR) gene in developing porcine conceptuses. Mol Reprod Dev 72(4):419-29.

de A. Camargo, L.S., A.M. Powell, V.R. Do Vale Filho, and R.J. Wall. 2005. Comparison of gene expression in individual preimplantation bovine embryos produced by in vitro fertilization or somatic cell nuclear transfer. Journal of Reproduction, Fertility and Development 17:487-469.

Guthrie, H.D., R.J. Wall, V.G. Pursel, J.A. Foster-Frey, D.M. Donovan, H.D. Dawson, G.R. Welch, and W.G. Garrett. 2005. Follicular expression of a human 2-cell leukaemia/lymphoma-2 (Bcl-2) transgene does not decrease atresia or increase ovulation rate in swine. Reprod. Fert. Dev. 17: 457-466.

Guthrie, H.D. and G.R. Welch. 2005. Impact of extender and cryopreservation on membrane and morphological integrity in boar sperm. Theriogenology 63:396-410.

Guthrie, H.D. and G.R. Welch. 2005. Effects of hypothermic liquid storage and cryopreservation on basal and induced membrane phospholipid disorder and acrosome exocytosis in boar spermatozoa. Reprod. Fert. Dev. 17:467-477.

Talbot, N.C., T.J. Caperna, A.M. Powell, A.D. Ealy, L.A. Blomberg, and W.M. Garrett. 2005. Isolation and characterization of a bovine visceral endoderm cell line derived from a parthenogenetic blastocyst. In Vitro Cellular and Developmental Biology - Animal 41:130-141.

Wall, R.J., A.M. Powell, M.J. Paape, D.E. Kerr, D.D.Bannerman, V.G. Pursel, K.D. Wells, N.C. Talbot, and H.W. Hawk. 2005. Genetically enhanced cows resist intramammary staphylococcus aureus infection. Nature Biotechnology 23(4):445-451.


VIRGINIA:

Walters, A.H., W.E. Eyestone, R.G. Saacke, R.E. Pearson, and F.C. Gwazdauskas. 2005. Bovine embryo development after IVF with spermatozoa having abnormal morphology. Theriogenology 63: 1925-1937.

Walters, A.H., R.G. Saacke, R.E. Pearson, and F.C. Gwazdauskas. 2005. The incidence of apoptosis after IVF with morphologically abnormal spermatozoa. Theriogenology 64: 1404-1421.


WISCONSIN:

Block, J. R.M. Rivera, M. Drost, F.D. Jousan, C.R. Looney, F.T. Silvestre, F.F. Paula-Lopes, O.M. Ocon, H. Rosson, T.R. Bilby, R.L. Monson, J.J. Rutledge and P.J. Hansen. 2005. Effects of bovine somatotropin and timed embryo transfer on pregnancy rates in non-lactating cattle. Vet. Record 156:175-176.

Fischer-Brown, A., A. Crooks, S. Leonard, R. Monson, D. Northey, and J.J. Rutledge. 2005. Parturition following transfer of embryos produced in two media under two oxygen concentrations. Animal Reproduction Science 87(3-4):215-228.

Franco, M., J. Block, F.D. Jousan, L.A. de Castro e Paula, A.M. Brad, J.M. Franco, R.L. Grisel, R.L. Monson, J.J. Rutledge and P.J. Hansen. 2006. Effect of transfer of one or two in vitro-produced embryos and post-transfer administration of gonadotropin releasing hormone on pregnancy rates of heat-stressed dairy cattle. Theriogenology (in press).

Wheeler, M.B., J.J. Rutledge, A. Fischer-Brown, T. VanEtten, S. Malusky and D.J. Beebe. 2005. Application of sexed semen technology to in vitro embryo production in cattle. Theriogenology 65: 219-227.

Zeringue, H.C., J.J. Rutledge and D.J. Beebe. 2005. Early mammalian embryo development depends on cumulus removal technique. Lab Chip 586-590.

B. BOOKS, INSTRUCTIONAL MEDIA, NON-REFEREED BOOK CHAPTERS, PROCEEDINGS FROM SCIENTIFIC MEETINGS, AND THESES/DISSERTATIONS

COLORADO:
Coutinho da Silva, M.A. 2005. Effects of Milk Proteins on Intracellular Ca2+ Concentration and Binding of Stallion Sperm to Zonae Pellucidae. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.

Lindbloom, S. 2005. Roles of EGF-Like Growth Factors and Phosphodiesterases in Initiation of Oocyte Maturation in the Equine Follicle. M.S. Thesis, Colorado State University, Fort Collins, CO.

Seidel, G.E., Jr. 2005. Mapping and sequencing the bovine genome  implications for the beef industry. Proc. 2005 Colorado Nutrition Round Table, pp. 1-9.

Seidel, G.E., Jr., E.M. Carnevale, E.L. Squires, J.F. Hasler and R.J. Mapletoft. 2005. New approaches to cryopreservation: Vitrification of bovine and equine embryos. Proc. VI Simposio Internacional Reproduccion Animal, Cordoba, Argentina, pp. 327-342.

Walker, D.J. 2005. A Simple Efficacious Method for Vitrifying Bovine Embryos. M.S. Thesis, Colorado State University, Fort Collins, CO.


CONNECTICUT:
Bormann, C. 2005. Optimization of Parthenogenetic Development of Bovine and Porcine Oocytes Activated With a Triple Activation Regimen. PhD Dissertation, University of Connecticut.

Chaubal, C. 2005. Optimization of Ovum Pick-Up in Cattle. PhD Dissertation, University of Connecticut.

Chang, C.-C. 2005. The Generation of a Haploid Genome from Somatic Cell and Growing Oocytes. PhD Dissertation, University of Connecticut.

Tian XC. 2005. The role of epigenetic modification in somatic cell cloning. Proceedings of the 2nd Asian Reproductive Technology Symposium, Bankok, Thailand, Nov 3-7, 2005, pp.


ILLINOIS:
Malusky, S.A., and M.B. Wheeler. 2005. Impact of biotechnology on animal production. VI Simposio Internacional de Reproduction Animal 6:23-51.

Wheeler, M.B. J.J. Rutledge, A. Fischer-Brown, S.A. Malusky, and D.J. Beebe. 2005. Application of novel in-vitro culture techniques and sexed semen technology in cattle VI Simposiom Internacional de Reproduction Animal 6:297-314.

IOWA:
Jahnke, Marianna Moreira. 2005. Strategies to Increase the Number of FSH-Receptive Follicles at the Initiation of Superovulation in Cattle. M.S. Thesis, Iowa State University, 60 pages.

Youngs, C.R. and R.A. Godke. 2005. Early Stage Mammalian Embryos. IETS Autotutorial Training CD. International Embryo Transfer Society. Savoy, IL. (In press).


LOUISIANA:
Godke , R.A. and K.R. Bondioli. 2005. Transvaginal oocyte aspiration and in vitro fertilization in beef cattle. Taurus 15:60-61.

Godke , R.A. and K.R. Bondioli. 2005. Transvaginal oocyte aspiration and in vitro fertilization in beef cattle. Proc 4th World Italian Beef Cattle Congress. pp. 41-51.

Landry, A.M. 2005. Recontruction of Nuclear Transfer Embryos in Goats and Cattle. PhD Dissertation. Louisiana State University. .

Shin, J. 2005. The Effect of Age and Sex on the Growth Pattern of Bovine Cell Lines. MS Thesis, Louisiana State University.

Youngs, C.R. and R.A. Godke. 2005. Early Stage Mammalian Embryos. IETS Autotutorial Training CD. International Embryo Transfer Society. Savoy, IL. (In press).


USDA- ARS (Beltsville):

Bannerman, D.D. and R.J. Wall. 2005. A novel strategy for the prevention of staphylococcus aureus-induced mastitis in dairy cows. Information Systems for Biotechnology (IBS) News Report.

Donovan, D.M., D.E. Kerr, and R.J. Wall. 2005. Engineering disease resistant cattle. Transgenic Research (in press).

Kerr, D.E., D.D. Bannerman, M.J. Paape,and R.J. Wall. 2005. Can we engineer genetic resistance to mastitis? Mastitis Council Meeting Proceedings. NMC 2005 Regional Meeting Proceedings, Burlington, VT, pp. 31-36.

New & Views commentary by Pascal Rainard, Tackling mastitis in dairy cows. Nature Biotechnology 23:430432, April 4, 2005.

Zuelke, K.A., J. McDougal, L.A. Blomberg, and E.L. Long. 2005. Molecular aspects of sperm oviduct interactions in poultry. British Andrology Society Annual Meeting, Sheffield, UK. November 15-19, 2006.


UTAH:
White, K.L., G.L. Woods, D.K. Vanderwall, G. Li, and T.D. Bunch. 2005. Cloning the Equine. In Epigenetic Risks of Cloning., CRC Press, Taylor & Francis Group, LLC. pp 59 - 70.



C. ABSTRACTS

ARKANSAS:
Elkins, D.A. and R.W. Rorie. 2006. Effect of social status of dairy heifers on expression of estrus and subsequent fertility. ASAS, Southern Section. (in press).

Post, N.M., D.L. Kreider, R.W. Rorie and T.D. Lester. 2005. Effects of supplemental progesterone in a timed insemination protocol in beef heifers. J. Anim. Sci. 83(Suppl 2):12.


CALIFORNIA:
Ducummon, C.C. and T. Berger. 2005 The GTPases Rho, Rac, and CDC42 are present in porcine sperm and at least one plays a role in mediating the zona pellucida induced acrosome reaction. Biol. Reprod. (Special Issue)

Pearl, C., T. Berger, and J.F. Roser. 2005 Immunolocalization of estrogen receptor alpha, estrogen receptor beta, And androgen receptor in the boar epididymis throughout development and after aromatase inhibition. Biol. Reprod. (Special Issue): 215.

Ramesh, R., T.Berger, E. At-Taras, C. Pearl, A.Conley, and J.F. Roser. 2005 Ontogeny of estrogen and androgen receptor expression in porcine Sertoli cells: effects of reducing local testicular estrogen synthesis using an aromatase inhibitor. Biol. Reprod. (Special Issue): 221.


COLORDAO:
Barcelo-Fimbres, M. and G.E. Seidel, Jr. 2005. Effects of glucose on fructose and metabolic regulators (DNP, PES, Cerulenin) on bovine embryo development and lipid accumulation. Biol. Reprod. (Special Issue), p. 149.

Campos-Chillon, L., T. Suh, E. Carnevale and G. Seidel, Jr. 2005. Effect of meiotic arrest by roscovitine and subsequent IVM time on developmental competence of immature bovine oocytes. Reprod. Fertil. Dev. 17:271 (abstr. #241).

Coutinho da Silva, M.A., G.E. Seidel, Jr., J.K. Graham, E.L. Squires and E.M. Carnevale. 2005. Effects of milk proteins and extracellular calcium concentration on the intracellular calcium concentration of stallion sperm. Theriogenology 64:802

De La Torre-Sanchez, J., D. Gardner, K. Preis and G. Seidel, Jr. 2005. Regulation of glucose metabolism to decrease lipid content of in vitro-produced bovine embryos. Reprod. Fertil. Dev. 17:218 (abstr. #135).

Horvath, G., L. Solti and G. Seidel. 2005. Vitrification of bovine oocytes treated with cholesterol-loaded methyl-$-cyclodextrin. Reprod. Fertil. Dev. 17:193-194 (abstr. #87).

Kutvolgyi, G., T. Suh, E. Carnevale and G. Seidel, Jr. 2005. Use of pentoxifylline and hyaluronic acid for stallion sperm separation. Reprod. Fertil. Dev. 17:310 (abstr. #319).

Kutvolgyi, G., T. Suh, E. Carnevale and G.E. Seidel, Jr. 2005. Morphologic evaluations after using pentoxifylline and hyaluronic acid for stallion sperm separation. Reprod. Dom. Anim. 40:407.

Preis, K., G. Seidel, Jr. and D. Gardner. 2005. Markers of oocyte competence during in vitro maturation. Reproduction Abstr. Series 32:48.

Seidel, G.E., Jr. 2005. Maintaining the mammalian embryo in vitro. Proc. Reproductive Science and Medicine Symposium, University of Saskatchewan, p. 5.

Seidel, G.E., Jr. 2005. Transgenic vertebrates -- the long road to practical application. Transgenic Res. 14:341.

Suh, T.K. and G.E. Seidel, Jr. 2005. Activation of protein kinase C and subsequent inhibition of protein phosphorylation improve embryonic development of bovine oocytes after ICSI. Biol. Reprod. (Special Issue), pp. 156-157.

Suh, T.K., G.E. Seidel, Jr. and S. Purcell. 2005. Effect of protein kinase C activation followed by kinase inhibition on embryonic development of in vivo-derived equine oocytes after ICSI. Proc. Havemeyer Symposium, p. 19.

Walker, D. and G. Seidel. 2005. Vitrification of bovine embryos without animal-derived products. Reprod. Fertil. Dev. 17:153 (abstr. #6).

Zhang, M., K.H. Lu and G.E. Seidel, Jr. 2005. Blastocyst development of male and female bovine embryos produced by IVF with flow cytometrically-sorted sperm. Reprod. Fertil. Dev. 17:306-307 (abstr. #312).

CONNECTICUT:
Chang, W.C., J. Xu, S. Jiang X.C. Tian, X. Yang, and F.L. Du. 2005. Effect of pre-equilibration procedure on the development potential of vitrified oocytes after IVF in cattle. Reprod Fert Dev 17: 190-191.

Chaubal, S., S. Bartolotta, M. Belski, G. Cimmino, H. Claus, C. Megyola, M. Orefice, B. Henderson, X. Yang, and X.C. Tian. 2005. Genetic influence on follicular development in cattle Reprod Fert Dev 17:251-252.

Curchoe, C., S. Zhang, Y. Bin, L. Yang, and X.C. Tian. 2005. Promoter-specific expression of the imprinted Igf2 gene in cattle Reprod Fert Dev 17: 259-260.

Everts, R.E., P. Chavatte-Palmer, A.A. Razzak, I. Hue, S .Rodriguez-Zas, X.C. Tian, X. Yang, J.-P. Renard, and H.A. Lewin. 2005. Global gene-expression profiling of cattle placentomes collected from term pregnancies derived by artificial insemination, in vitro fertilization and nuclear transfer. Proc. Plant and Animal Genome annual meeting.

Everts, R.E., A. Razzak, P. Chavatte-Palmer, I. Hue, C.A. Green, R. Oliveira, S. Rodriguez-Zas, X.C. Tian, X. Yang, J.-P. Renard, and H.A. Lewin. 2006. Global gene expression profiling distinguishes placentomes derived by artificial insemination from those pregnancies derived by either in vitro fertilization or nuclear transfer. Proc. Plant and Animal Genome annual meeting.

Jiang, L., R.S. Prather, P. Jobst, D. Ayares, X. Yang, and X.C. Tian. 2006. Expression of growth-related imprinted genes in decreased newborn and surviving cloned piglets. Reprod. Fertil. Dev. (in press).

Kim, C., Y. Ma, C.-C. Chang, X. Yang, T. Rasmussen, and X.C. Tian. 2006. The typical pattern of histone macroH2A1 in preimplantation bovine embryos following activation and in vitro fertilization. Reprod. Fertil. Dev. (in press).

Lonergan, P., A.C.O. Evans. E. Boland, D. Rizos, L.-Y. Sung, F. Du, S. Chaubal, S. Fair, E. Scraggs, P. Duffy, X. Yang, and X.C. Tian. 2005. Pregnancy and fetal characteristics after transfer of vitrified in vivo and cloned bovine embryos Reprod Fert Dev 17:173-174.

Smith, S.L., L.-Y. Sung, R. Page, B. Henderson, F. Du, R. Everts, T. Nedambale, S. Rodriguez-Zas, J.-P. Renard , H. Lewin, X. Yang, and X.C. Tian. 2006. Global gene expression profiling of single bovine embryos reveals significant effects of in vitro maturation, fertilization and culture on gene expression. Reprod. Fertil. Dev. (in press).

Sung, L.-Y., F. Du, B.S. Jeong, C.C. Chang, J. Xu, X.C. Tian, and X. Yang. 2005. Premature chromosome condensation (PCC) is not essential for bovine somatic nuclear reprogramming Reprod Fert Dev 17:183-184.


ILLINOIS:
Davidson, T.R., C.E. Ferguson, J. Lynn, T. Chapman, K. Hebert, M.B. Wheeler, K. Bondioli, and R\.A. Godke. 2005. Evaluation Of apoptosis in bovine embryos by fluorescent labeling of caspases-3 And -7. Reproduction, Fertility and Development 17:217-218.

Donovan, S.M., J.L. Hartke, M.H. Monaco, and M.B. Wheeler. 2005. Insulin-like growth factor-I and piglet intestinal development in biomedical models and transgenics. Swine in Biomedical Research Conference, Chicago, IL January 27-29, 2005.

Ferguson, C.E., T.R. Davidson, M.R.B. Mello, A.S. Lima, D.J. Kesler, M.B. Wheeler, and R.A. Godke. 2005. Evidence of a direct effect of P4 on IVF-derived bovine 8-cell embryos. Reproduction, Fertility and Development 17:219.

Fischer-Brown, A., G. Barquero, S. Clark, C. Ferguson, F. Ireland, N. Jensen, S. Lane, B. Lindsey, P. Lopes, R. Monson, D. Northey, A. Reeder, J. Rutledge, M. Wheeler, and D. Kesler. 2005. Twin vs. single transfer of IVP Holstein heifer embryos to beef recipients. Reproduction, Fertility and Development 17:230.

Hartke, J., M. Monaco, R. McCusker, M. Wheeler, and S. Donovan. 2005. Small intestinal IGF-I binding protein (IGFBP)-2 and -5 and IGF receptors in piglets suckling IGF-I transgenic sows. Journal of Dairy Science 88 (Suppl. 1): W102, 282.

Lima, A.S., C.E. Ferguson, and M.B. Wheeler. 2005. Embryo development in microdrops and microchannnels: Comparison of NCSU23 sequential and non-sequential culture medium. Reproduction, Fertility and Development 17:274-275.

Mello, M.R.B., C.E. Ferguson, A.S. Lima, and M.B. Wheeler. 2005. In Vitro development of bovine embryos cultured in KSOM, CR1AA or KSOM/CR1aa. Reproduction, Fertility and Development 17:221-222.

Monaco, M., M. Wheeler, and S. Donovan. 2005. Transgenic over-expression of IGF-I modulates the synthesis and secretion of pig milk IGFBP-2 and -5 in the early and post-lactation periods. Journal of Dairy Science 88 (Suppl. 1): W101, 282.

Sendemir-Urkmez, A., C. Kearney, S. Malusky, M.B. Wheeler, and R. Jamison. 2005. Osteoblastic differentiation of porcine derived mesenchymal stem cells on 3D scaffolds for bone tissue engineering. Proc. Swine in Biomedical Research Conference, January 27 - 29, 2005, Chicago, IL., p 12, Abtract BE2.


INDIANA:
Krisher, R.L.. 2005. Inhibition of the pentose phosphate pathway results in meiotic arrest in porcine oocytes that can be overcome by the addition of pathway cofactors and end products. Reprod. Fertil. Dev. 17:293 (abstr. 287).

Krisher, R., M. Wulster-Radcliffe, M. Spurlock, and M. Sturek. 2006. The Ossabaw pig as a model of polycystic ovary syndrome. Experimental Biology (in press).

Paczkowski, M., D. Terry, and R. Krisher. 2005. Differential protein expression of in vitro matured porcine oocytes derived from gilts and sows. Biol. Reprod. Special Issue 2005 abstr. 526, p. 197.

Paczkowski, M., D. Terry, C. Bidwell, and R. Krisher. 2006. Differential gene and protein expression in gilt and sow derived oocytes. J. Anim. Sci (Suppl. ):(in press)

Tubman, L., A. Peter, and R.L. Krisher. 2005. Effect of energy substrates on metabolism and meiosis of porcine oocytes during in vitro maturation. Reprod. Fertil. Dev. 17:301 (abstr. 301).

Tubman, L., A. Peter, and R.L. Krisher. 2006. Pentose phosphate pathway activity controls nuclear maturation of porcine oocytes. Reproduction, Fertility and Development (in press).

LOUISIANA:
Davidson, T.R., C.E. Ferguson, J. Lynn, T. Chapman, K. Hebert, M.B. Wheeler, K. Bondioli and R.A. Godke. 2005. Evaluation of apoptosis in bovine embryos by fluorescent labeling of caspases-3 and -7. Reprod. Fertil. Develop. 17:217-218.

Ferguson, C.E., T.R. Davidson, M.R.B. Mello, A.S. Lima, D.J. Kesler, M.B. Wheeler and R.A. Godke. 2005. Evidence of a direct effect of P4 on IVF-derived bovine 8-cell embryos. Reprod. Fertil. Develop. 17:219.

Ferrer, M.S., S.K. Lyle, B.E. Eilts, R. Godke and D.L. Paccamonti. 2005. Post-mating endometritis after hysteroscopic insemination in the mare. Proc. Soc for Theriogenology (In press).

Giraldo, A.M., J. W. Lynn, E.C. Pope, R. A. Godke and K. R. Bondioli. 2005. Lifespan and chromosomal stability of bovine and porcine fetal fibroblast cells cultured in vitro. Reprod. Fertil. Develop. 17:167.

Giraldo, A.M., J.A. Jenkins, J.W. Lynn, R.A. Godke and K.R. Bondioli. 2006. Histone phosphorylation patterns and chromosomal stability of cultured bovine fibroblasts. Reprod. Fertil. Develop. (in press)

Guerrero, C.A., S.P. Leibo, K.R. Bondioli and R.A. Godke. 2006. Effect of cryoprotective agent addition and removal at 4°C on motility and plasma membrane integrity of bovine caudal epididymal spermatozoa. Reprod. Fertil. Develop. (in press).

Landry, A.M., H. DiMaggio, W. Burnside, L. Sarradet, J. Saenz, D.J. Landry, L.R. Gentry, K. Bondioli and R.A. Godke. 2005. The effect of hCG on circulating progesterone in goats at the end of the breeding season. Southern Section, Amer. Soc. Anim. Sci. 45:13.

Landry, A.M., M. Murakami, R.S. Denniston, J.L. Williams, Y. Echelard, and R.A. Godke. 2005. Development of bovine aggregate embryos constructed from nuclear transfer embryos and electrofused IVF-derived embryos. Reprod. Fertil. Develop. 17:228.

McAllister, J., S. Bushnell, M. Purpera, M. Sansinena, A. Landry, B. Olcott, E. Loetz, R.S. Denniston, K. Bondioli and R. A. Godke. 2006. Freezing embryos form Angora goats for the USDA Animal Germplasm Cryobank. J. Anim. Sci. (in press).

Moisan, A.E., E.L.Chamberlain, S.P. Leibo, B.L. Dresser, K. Bondioli and R.A. Godke. 2005. Blastocyst formation from vitrified bovine oocytes, zygotes and 2-cell embryos. Reprod. Fertil. Develop. 17:196.

Moisan, A.E., S.P. Leibo, J.W. Lynn, M.C. Gómez, C. E. Pope, K Bondioli, L. Dresser, R. A. Godke. 2006. Blastocyst development from felid oocytes injected with dehydrated spermatozoa. Reprod. Fertil. Develop. (in press).

Nel-Themaat, L., M.C. Gomez, A. Cole, G. Wirtu, K.R. Bondioli, B.L. Dresser and R.A. Godke and C.E. Pope. 2005. Somatic cell isolation from semen by percoll gradients. Reprod. Fertil. Develop. 17: 314-315.

Roberts, J.L., R.P. DelVecchio, G.T. Gentry, Jr., D. Sanders, P.E. Humes and R.A. Godke. 2005. Effect of melengestrol acetate (MGA) and monensin supplementation on puberty and pregnancy rates in crossbred beef heifers. Southern Section, Amer. Soc. Anim. Sci. 45:12.

Sansinena, M., J. Lynn, R. Denniston and R. Godke. 2005. Ooplasmic transfer after interspecies nuclear transfer: Presence of foreign mitochondria, pattern of migration, and effect on embryo development. Reprod. Fertil. Develop. 17:182.

Taylor, P.J., S.A. Taylor, M. Sansinena and R.A. Godke. 2006. Using a novel coaxial microinjection system and embryo vitrification to produce llama (Lama glama) pregnancies: A preliminary study. Reprod. Fertil. Develop. (in press).

MARYLAND:
He, S., D. Pant, S. Bischoff, W. Gavin, D. Melican, and C.L. Keefer. 2005. Expression of pluripotency-determining factors Oct-4 and Nanog in preimplantation goat embryos. Reprod. Fert. Dev 17:203 (abst. 105).

Pant, D., and C.L. Keefer. 2006. Gene expression in cultures of inner cell masses isolated from in vitro produced and in vivo derived bovine blastocysts. Reprod. Fert. Dev. (in press).

NORTH CAROLINA:
Farin, C.E., J.R. Sommer, K.F. Rodriguez, R.M. Petters, and J.E. Alexander. 2006. Functional analysis using short-interfering (si)RNA of TRAM-6, a novel transcript associated with oocyte maturation in cattle. Reprod Fert Dev (in press).

Piedrahita, J.A. , S. Bischoff, J.L. Estrada, B.A. Freking, D. Nonneman, A.C. Martin, B. Mir, G. Rohrer, and S. Tsai. 2006. Use of porcine parthenotes and gene expression profiling using microarrays for identification of imprinted genes. Reprod Fert Dev (abstract in press).

USDA-ARS (Beltsville):
Blomberg, L.A., T.S. Sonstegard, C.P. Van Tassell, and K.A. Zuelke. 2005. Transitions in the gene expression profiles (transcriptome) during elongation of the porcine conceptus trophectoderm. Reprod. Fertil. Devel.

Blomberg, L.A., J.R. Miles, N.C. Talbot, W. Garrett, and K.A. Zuelke. 2006. Unique expression patterns of differentiation, growth and cell structure factors in the elongating porcine conceptus. Reproduction, Fertility and Development 18(2):259-260.

Guthrie, H.D. and G.R. Welch. 2005. New assays for reactive oxygen species (ROS) formation and mitochondrial membrane potential in fresh and cryopreserved boar spermatozoa. 7th International Conference on Pig Reproduction June 12-16, 2005, Kerkade, The Netherlands.

Guthrie, H. D., J.A. Long, G.R. Welch, and N.E. Grimm. 2006. Treatment of boar sperm with respiration inhibitor menadione: effects on motility, reactive oxygen species (ROS), mitochondrial transmembrane potential (MMP), and ATP content. Reproduction, Fertility and Development 18(2):259-260.

Miles, J. R., L.A. Blomberg, B.A. Freking, and K.A. Zuelke. 2006. Lines of pigs selected for component traits of litter size exhibit differential gene regulation at the onset of embryo (trophoectoderm) elongation. Reproduction, Fertility and Development 18(2):259-260.


UTAH:
Aston, K., H. Johnson,, K.L. White, and Q.Winger. 2005. AP-2g Transcription factor during bovine embryo development and placenta formation. 38rd Annual Meeting of the Society for the Study of Reproduction, Quebec City, Quebec. Biol. Reprod.

Aston, K., G-P. Li, B. Hicks, B. Sessions, B. Patel. D. Hammon. T. Bunch, and K. White. 2005. The developmental competence of bovine nuclear transfer embryos derived from cow versus heifer cytoplasts. USU Graduate Student Symposium, Utah State University, Logan UT. April 13, 2005, 8.

Li, G.P., T.D. Bunch, K.L. White, B.R. Sessions, K.I. Aston, and B.J. Pate. 2005. Parthenogenetic and nuclear transfer embryo development of bovine oocytes denuded and centrifuged at different times during maturation. Mole. Biol. Cell 16:527.

Pate, B.J., K.L. White, D.B. DeWald, L.F. Rickords, G. Li, K.I. Aston, B.R. Sessions, and T.D. Bunch. 2005. Involvement of tyrosine kinases, specifically Src family kinases, focal adhesion kinase (Fak) and agonist-induced PLC in the activation and development of bovine oocytes. American Society for Cell Biology., San Francisco. December 10 - 14.

Pate, B.J., K.L. White, D. Chen, P. Desai, K.I. Aston, B.R. Sessions, and B. C. Weimer. 2005. A novel approach to identify bovine sperm membrane proteins that interact with receptors on the vitelline membrane of bovine oocytes. Mole. Biol. Cell 16:519.

Sessions, B.R., Aston, K.I., Davis, A.P., Pate, B.J. and K. L. White. 2005. Effects of amino acid substitutions in and around the Arginine-Glycine-Aspartic acid (RGD) sequence on fertilization and parthenogenetic development in mature bovine oocytes. Mole. Biol. Cell 16:2549.


VIRGINIA:
Walters, A.H., R.G. Saacke, R.E. Pearson, and F.C. Gwazdauskas. 2005. The incidence of programmed cell death after in vitro fertilization (IVF) with morphologically abnormal bovine spermatozoa. J. Dairy Sci. 88: (Suppl. 1) 137.

Walters, A.H., R.G. Saacke, R.E. Pearson, and F.C. Gwazdauskas, F. C. Assessment of pronuclear formation following IVF with bovine spermatozoa having abnormal morphology after thermal


D. MISCELLANEOUS PUBLICATIONS (SEMI-TECHNICAL/LAY PUBLICATIONS):

ARKANSAS:
Influence of social hierarchy on the expression of estrus and subsequent fertility of dairy heifers. Ark. Anim. Aci., Res. Series 535: 67-68.


LOUISIANA:
Roberts, J.L., R.P. Del Vecchio, G.T. Gentry Jr., D. Sanders, P.E. Humes and R.A. Godke. 2005. Priming effect of melengestrol acetate (MGA) and monensin on puberty and subsequent reproductive performance in crossbred beef heifers. LSU AgCenter Beef Cattle Rep. 33:54-58.


07/05/2007

1. REFEREED ARTICLES/BOOK CHAPTERS

Aston, K., G-P. Li, B. Hicks, B. Sessions, B. Pate, D. Hammon, T. Bunch, and K. White. 2006. Effect of the time interval between fusion and activation on nuclear state and development in vitro and in vivo of bovine somatic cell nuclear transfer embryos. Reproduction 131:1-8.

Aston, K.I., G-P. Li, B.A. Hicks, B.R. Sessions, B.J. Pate, D.S. Hammon, T.D. Bunch, and K.L. White. 2006. The developmental competence of bovine nuclear transfer embryos derived from cow versus heifer cytoplasts. Anim. Reprod. Sci. 95:234-243.

At-Taras, E. E., T. Berger, M. J. McCarthy, A. J. Conley, B. J. Nitta-Oda, and J. F. Roser. 2006. Reducing estrogen synthesis in developing boars increases testis size and total sperm production. J Androl 27: 552-559.

At-Taras, E. E., A. J. Conley, T. Berger, and J. F. Roser. 2006. Reducing estrogen synthesis does not affect gonadotropin secretion in the developing boar. Biol Reprod 74: 58-66.

At-Taras, E. E., I. Kim, T. Berger, A. J. Conley, and J. F. Roser. 2006. Reducing endogenous estrogen during development alters hormone production by porcine Leydig cells and seminiferous tubules domestic animal endocrinology Domest Anim Endocrinol doi:10.1016/j.domaniend.2006.11.003 (in press).

Bakst, M., Akuffo, V., Trefil, P., and Brillard, J.P. 2006. Morphological and histochemical characterization of the seminiferous epithelial and Leydig cells of the turkey. Ani. Reprod. Sci. 97:303-313.

Bakst, M.R., Long, J.A., and Kramer, M.H. 2006. Impact of fenbendazole on turkey semen quality. J. of Appl. Poultry Res. 15:307-311.

Blomberg, L.A., Garrett, W.M., Guillomot, M., Miles J.R., Sonstegard, T.S., Van Tassell, C.P., and Zuelke, K.A. 2006. Transcriptome profiling of the tubular porcine conceptus identifies the differential regulation of growth and developmentally associated genes. Mol. Reprod. Dev. 73:1491-502.

Bou Khalil, M., K. Chakrabandhu, H. Xu, W. Weerachatyanukul, M. Buhr, T. Berger, E. Carmona, N. Vuong, P. Kumarathasan, P. T. Wong, D. Carrier, and N. Tanphaichitr. 2006. Sperm capacitation induces an increase in lipid rafts having zona pellucida binding ability and containing sulfogalactosylglycerolipid. Dev Biol 290: 220-235.

Campos-Chillon, L. F., D.J. Walker, J.F. De La Torre-Sanchez, and G.E. Seidel, Jr. 2006. In vitro assessment of a direct transfer vitrification procedure for bovine embryos. Theriogenology 65:1200-1214.

Chang C-C, Nagy ZP, Abdelmassih R, Liu J-L, Yang X, Tian XC. 2006. Meiotic spindle formation and function following insertion of mitotic chromosomes into GV stage mouse oocytes. RMBonline 12:213-221.

Chaubal SA, Molina JA, Ohlrichs CA, Ferre LB, Faber DC, Bols PEJ, Riesen J, Tian XC. Yang X. 2006. Different transvaginal ovum pick-up techniques in cows to optimize oocyte retrieval and embryo production over a fixed period of time. Theriogenology 66:1631-1648.

De La Torre-Sanchez, J. F., K. Preis, and G.E. Seidel, Jr. 2006. Metabolic regulators of in vitro-produced bovine embryos I. Effects of metabolic regulators at different glucose concentrations with embryos produced by semen from different bulls. Reprod. Fertil. Develop. 18:585-596.

De La Torre-Sanchez, J.F., D.K. Gardner, K. Preis, J. Gibbons, and G.E. Seidel, Jr. 2006. Metabolic regulation of in vitro-produced bovine embryos II. Effects of phenazine ethosulfate, sodium azide, and 2,4-dinitrophenol during post-compaction development on glucose metabolism and lipid accumulation. Reprod. Fertil. Develop. 18:597-607.

Du F, Shen P, Xu J, Sung L, Jeong B-S, Nedambale TL, Riesen J, Tian XC, Cheng WTK, Lee S-N, Yang X. 2006. Phytohemagglutinin-cell agglutination agent and embryo vitrification improves the efficiency of somatic cloning in cattle (Bos Taurus). Theriogenology 65: 642-657.

Ducummon, C.C. and T. Berger. 2006. Localization of the rho GTPases and some rho effector proteins in the sperm of several mammalian species. Zygote 14: 249-257.

Farin CE, Rodriguez KF, Alexander JE, Hockney JE, Herrick JR, and Kennedy-Stoskopf S. 2006. The role of transcription in EGF- and FSH-mediated oocyte maturation in vitro. Anim Reprod Sci, 2006 (October 13, Epub ahead of print).

Farin PW, Moore K, and Drost M. Assisted reproductive technologies in cattle. 2006. In: (RS Youngquist and WR Threlfall, Eds.) Current Therapy in Large Animal Theriogenology, 2nd Ed., Elsevier, Inc., (in press).

Farin PW, Piedrahita JA, and Farin CE. 2006. Errors in development of fetuses and placentas from in vitro-produced bovine embryos. Theriogenology 65:178-191

Ford, J.A., S.G. Clark, E.M. Walters, M.B. Wheeler, W.L. Hurley 2006. Estrogenic effects of genistein on reproductive tissues of ovariectomized gilts. J. Anim. Sci. 84:834842.

Giraldo, A., J. Lynn, R.A. Godke and K. Bondioli.2006. Proliferation characteristics and chromosomal stability of bovine donor cells for nuclear transfer. Mol. Reprod. Dev. 73:1230-1238.

He, S., D. Pant, A. Schiffmacher, S. Bischoff, D. Melican, W. Gavin, and C. Keefer. 2006. Developmental expression of pluripotency determining factors in caprine embryos: Novel pattern of nanog protein localization in the nucleolus. Mol Reprod Dev 73: 1512-1522.

Herrick, J.R., Behboodi, E., Memili, E., Blash, S., Echelard, Y., Krisher, R.L. 2006. Metabolism, protein content and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate. Mol. Reprod. Dev. 73:256-266.

Herrick, J.R., Brad, A.M., Krisher, R.L. 2006. Chemical manipulation of glucose metabolism in porcine oocytes: Effects on nuclear and cytoplasmic maturation in vitro. Reproduction. 131: 289-298.

Herrick JR, Lane M, Gardner DK, Behboodi E, Memili E, Blash S, Echelard Y, Krisher RL. 2006. Metabolism, protein content, and in vitro embryonic development of goat cumulus-oocyte complexes matured with physiological concentrations of glucose and L-lactate. Mol Reprod Dev 73:256-266.

Horvath, G., and Seidel, G. E. Jr. 2006. Vitrification of bovine oocytes after treatment with cholesterol-loaded methyl-²-cyclodextrin. Theriogenology 66:1026-1033.

Jiang L, Jobst P, Lai L, Samuel M, Ayares D, Prather RS, Tian XC. 2006. Expression of Growth-Regulating Imprinted Genes in Cloned Piglets. Cloning Stem Cell (in press).

Jiang L, Lai L, Samuel M, Prather RS, Yang X, Tian XC. 2006. Expression of X-linked Genes in Deceased Neonates and Surviving Cloned Female Piglets. Mol Reprod Dev (in press).

Keefer, C.L., Pant, D., Blomberg, L.A., and Talbot, N.C. 2006. Challenges and prospects for the establishment of embryonic stem cell lines of domesticated ungulates. Anim. Reprod. Sci. Oct 14; [Epub ahead of print].

Lazaris, A., R. Keyston, C. N. Karatzas, and C. L. Keefer. 2006. Transgenesis using nuclear transfer in goats. Methods Mol Biol 348: 213-226.

Li, G-P., T.D. Bunch, K.L. White, L. Rickords, Y. Liu, and B. R. Sessions. 2006. Denuding and centrifugation of maturing oocytes alters oocyte spindle integrity and the ability of cytoplasm to support parthenogenetic and nuclear transfer embryo development. Mol. Reprod. Dev. 73:446-451.

Li, G., J. Saenz, R.A. Godke and R.V. Devireddy. 2006. Effect of glycerol and cholesterolloadedcyclodextrin on freezing induced water loss in bovine spermatozoa. Reproduction 131:875-886

Lindbloom, S.L., T.A. Farmerie, C.M. Clay, G.E. Seidel, Jr., and E.M. Carnevale. 2006. Roles of EGF-like growth factors and phosphodiesterases in initiation of oocyte maturation in the equine follicle. Anim. Reprod. Sci. (in press).

Liu, Y., G-P. Li, L.F. Rickords, K.L. White, B.R. Sessions, K.I. Aston, T.D. Bunch. 2006. Effect of nicotine on in vitro maturation of bovine oocytes. Reprod. Biol. Endocrinol. Online citation: http://dx.doi.org/10.1016/j.anireprosci.2006.11.013.

Long, J.A. 2006. Avian semen cryopreservation: What are the biological challenges? J. Poultry Sci. 85:232-236.

Long, J.A., and H.D. Guthrie. 2006. Validation of a rapid, large-scale assay to quantify ATP levels in spermatozoa. Theriogenology 65:1620-1630.

Marshall, K.M., W.L. Hurley, R.D. Shanks, M.B Wheeler. 2006. Effects of suckling intensity on milk yield and piglet growth from lactation-enhanced gilts. J. Anim. Sci. 84:23462351.

McCarthy, M. J., E. E. At-Taras, C. A. Pearl, B. S. Nitta-Oda, J. F. Roser, A. J. Conley, and T. Berger. 2006. Suppression of endogenous estrogen during development affects porcine epididymal sperm maturation. Mol Reprod Dev 73: 1122-1128.

Memili E, Peddinti D, Shack LA, Nanduri B, McCarthy F, Sagirkaya H and Burgess SC. 2006. Global proteome of bovine germinal vesicle oocytes and cumulus cells. Reproduction. (in press).

Michael, D.D., Alvarez, I.M., Ocon, O.M., Powell, A.M., Talbot, N.C., Johnson, S.E., and Ealy A.D. 2006. Fibroblast growth factor-2 is expressed by the bovine uterus and stimulates interferon-tau production in bovine trophectoderm. Endocrinology 147:3571-3579.

Miles JR, Farin CE, Rodriguez KF, Alexander JE, and Farin PW. 2005. Effects of embryo culture on angiogenesis and morphometry of bovine placentas during early gestation. Biol Reprod 73:663-671.

Mir B, Zaunbrecher G, Archer GS, Friend T, and Piedrahita JA. 2005. Progeny of somatic cell nuclear transfer (SCNT) clones are phenotypically similar to non-cloned pigs. Cloning and Stem Cells 7:119-125.

Misirlioglu M, Page G, Sagirkaya H, Kaya A, Parrish JJ, First NL and Memili E. 2006. Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos. Proc. Natl. Acad. Sci, USA. 103(50): 18905-10.

Mocklin, C.M., D.L. Paccamonti, B.E. Eilts, S.K. Lyle, E.G.H. Wouters, L.R. Gentry and R.A. Godke. 2006. Effect of post-ovulatory PGF2± or cloprostenol on plasma progesterone concentration in mares. Anim. Reprod. Sci. 94:220-222.

Murakami, M., C.E. Ferguson, O. Perez, A. Boediono, D. Paccamonti, K. Bondioli and R. A. Godke. 2006. Transfer of inner cell mass cells derived from bovine nuclear transfer embryos into the trophoblast of bovine in vitro-produced embryos. Cloning & Stem Cells 8:52-60.

Nedambale T.L., Du F, Xu J, Chaubal SA, Dinnyes A,Groen W, Faber D, Dobrinsky JR, Yang X, Tian XC. 2006. Prolonging bovine spermoocyte incubation in modified medium 199 improves embryo development rate and the viability of vitrified blastocysts. Theriogenology 66:1951-1960.

Nedambale T.L., Du FL, Yang X, Tian XC. 2006. Effects of culture media on the development of in vitro-derived bovine embryos, and of ²-mercaptoethanol on post-vitrification survival. Anim Reprod Sci 93:61-75.

Nel-Themaat, L., G.D. Harding, J.E. Chandler, J.F. Chenevert, P. Damiani, J.M. Fernandez, P.E. Humes, C.E. Pope and R.A. Godke. 2006. Quality and freezability of first and second ejaculates collected from endangered gulf coast native rams. Anim. Reprod. Sci. 95:251-261.

Pate, B.J., K.L. White, Q.A. Winger, L.F. Rickords, K.I. Aston, B.R. Sessions, G-P Li, K.D Cambell, B. Weimer and T.D. Bunch. 2006. Specific integrin subunits in bovine oocytes, including novel sequences for Alpha 6 and Beta 3 subunits.. Mole. Reprod. Devel. (Published Online  Oct12, 2006), (in press).

Pearl, C. A., T. Berger, and J. F. Roser. 2006. Estrogen and androgen receptor expression in relation to steroid concentrations in the adult boar epididymis. Domest Anim Endocrinol. doi:10.1016/j.domaniend.2006.09.003 (in press).

Powell, S. A., B. B. Smith, K. I. Timm, and A. R. Menino, Jr. 2006. Estradiol production by preimplantation blastocysts and increased serum progesterone following estradiol treatment in llamas. Anim. Reprod. Sci. (in press).

Powell, S.A., B.B. Smith, K.I. Timm, and A.R. Menino, Jr. 2006.Expression of estrogen receptors a and b in the corpus luteum and uterus from non-pregnant and pregnant llamas. Mol. Repod. Dev. (in press)

Preis, K.A., G.E. Seidel, and D.K. Gardner. 2006. Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation. Molec. Reprod. Develop. (DOI 10.1002.mrd) (in press)

Purcell, S. H., Seidel, G.E. Jr., McCue, P.M., and Squires, E.L. 2006. Aspiration of oocytes from transitional, cycling, and pregnant mares. Anim. Reprod. Sci. (in press).

Ramesh, R., C. A. Pearl, E. At-Taras, J. F. Roser, and T. Berger. 2006. Ontogeny of androgen and estrogen receptor expression in porcine testis: Effect of reducing testicular estrogen synthesis. Anim Reprod Sci. doi:10.1016/j.anireprosci.2006.10.025 (in press).

Rodriguez KF, Blomberg LA, Zuelke KA, Miles JR, Alexander JE, and Farin CE. 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiol Genomics 27: 318-327.

Sagirkaya H, Misirlioglu M, Kaya H, Parrish JJ, First NL and Memili E. 2006. Developmental potential of bovine oocytes cultured in defined maturation medium. Animal Reproduction Science. Oct 17; [Epub ahead of print] PMID: 17052869 [PubMed - as supplied by publisher]

Sagirkaya H, Misirlioglu M, Kaya H, Parrish JJ, First NL and Memili E. 2006. Developmental and molecular correlates of bovine preimplantation embryos. Reproduction 131: 895-904

Sansinena, M. J., S.A. Taylor, P.J. Taylor, E.E. Schmidt, R.S. Denniston and R.A. Godke. 2006. In vitro production of llama (Lama glama) embryos by intracytoplasmic sperm injection: Effect of chemical activation treatments and culture conditions. Anim. Reprod. Sci. (In press).

Schenk, J. L., Suh, T. K., and Seidel, G. E. Jr. 2006. Embryo production from superovulated cattle following insemination of sexed sperm. Theriogenology 65:299-307.

Seidel, G. E. Jr. 2006. Modifying oocytes and embryos to improve their cryopreservation. Theriogenology 65:228-235.

Seidel, G. E., Jr., and Walker, D. J. 2006. Pregnancy rates with embryos vitrified in 0.25-ml straws. J. Reprod. Develop. 52 Suppl): S71-S76.

Sessions, BR, Aston, KI, Davis, AP, Pate, BJ and KL White. 2006. Effects of Amino Acid Substitutions in and Around the Arginine-Glycine-Aspartic Acid (RGD) Sequence on Fertilization and Parthenogenetic Development in Mature Bovine Oocytes. Mol. Reprod. Dev. 73:651-657.

Sung L-Y, Gao S, Shen H, Yu H, Song Y, Smith SL, Chang C-C, Inoue K, Kuo K, Lian J, Li A, Tian XC, Tuck DP, Weissman SM, Cheng T, Yang X. 2006. Cloned mouse pups can be derived from adult stem cells as well as terminally differentiated somatic cells. Nature Genetics 38:1323-8.

Sung LY, Shen PC, Jeong BS, Xu J, Chang CC, Cheng WT, Wu JS, Lee SN, Broek D, Faber D, Tian XC, Yang X, Du F. 2006. Premature Chromosome Condensation Is Not Essential for Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer. Biol Reprod 2006 Nov 15; [Epub ahead of print]

Suteevun T, Parnpai R, Smith SL, Chang C-C, Muenthaisong S, and XC Tian. 2006. Epigenetic characteristics of cloned and in vitro fertilized swamp buffalo (Bubalus bubalis) embryos. J Anim Sci 84:2065-2071.

Suteevan T, Smith S, Muentaisong S, Yang X, Parnpai R, Tian XC. 2006. Anomalous mRNA levels of chromatin remodeling genes in swamp buffalo (Bubalus bubalis) cloned embryos. Theriogenology 65:1704-1715.

Tanphaichitr, N., E. Carmona, M. Bou Khalil, H. Xu, T. Berger, and G. L. Gerton. 2007. New insights into sperm-zona pellucida interaction: Involvement of sperm lipid rafts. Front Biosci 12: 1748-1766.

Trefil, P., Micakova, A., Mucksova, J., Hejnar, J., Brillard, J.P., Bakst, M. Kalina, J., and Poplstein, M. 2006. estoration of spermatogenesis and male fertility by transplantation of dispersed testicular cells in the chicken. Biol. Reprod. 75: 575  581.

Tsai S, Cassady J, Freking D, Nonneman G. Rohrer G, and Piedrahita JA. 2006. Annotation of Affymetrix porcine genome microarray chip. Animal Genetics 37:423-424.

Tsai S, Mir B, Martin AC, Estrada JL, Bischoff SR, Hsieh WP, Cassady JP, Freking BA, Nonneman DJ, Rohrer GA, and Piedrahita JA. 2006. Detection of transcriptional difference of porcine imprinted genes using different microarray platforms. BMC Genomics. 7:328-334.

Vanderwall, DK, Woods, GL, Roser, JF, Schlafer, DH, Sellon, DC, Tester, DF, and White, KL. 2006. Equine Cloning: Applications and Outcomes. Reprod. Fertil. Devel. 18:91-98.

Walker, D. J., Campos-Chillon, L. F., and Seidel, G. E. 2006. Vitrification of in vitro-produced bovine embryos by addition of ethylene glycol in one step. Reprod. Domest. Anim. 41:467-471

Wheeler, M.B., A.S. Lima, T. VanEtten, M.R.B. Mello. 2006. Transgenic Animals for Biomedicine and Agriculture. In (Rodolfo L. de la Sota and Gabriel A. Bo, Eds.) Bovine Theriogenology 23 pgs.

Wheeler, M.B., M.R.B. Mello, A.S. Lima, T. VanEtten. 2006. Modification of the Domestic Animal Genome. In (Rodolfo L. de la Sota and Gabriel A. Bo, Eds.) Bovine Theriogenology 33 pgs.

Wheeler, M.B., J.J Rutledge, A. Fischer-Brown, T. VanEtten, S. Malusky, D.J. Beebe. 2006. Application of sexed semen technology to in vitro embryo production in cattle. Theriogenology 65:219227.

White, K.L., M. Passipieri, T.D. Bunch, K.D. Cambell and B.J. Pate. 2006. Effects of arginine-glycine-aspartic acid (RGD containing snake venom peptides on parthenogenetic development and in vitro fertilization of bovine oocytes. Mol. Reprod. Dev. 74:88-96.

Woodard, J., A. Hilldore, , M. B. Wheeler, S. G. Clark, S. Lan, R. Jamison, A.J. Wagoner Johnson. 2006. The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28:4554.

Xu J, Guo ZQ, Nedambale TL, Zhang JX, Schenk J, Tian XC, Yang X, Du F. 2006. High developmental potential of vitrified Holstein cattle embryos fertilized in Vitro with sex-sorted sperm. J Dairy Sci 89:2510-8.

Youngs, C.R. and R.A. Godke. 2006. Biotechnology: Cattle Embryo Transfer Technology. In (D.R. Heldman, A. Bridges, D. Hoover, and M.B. Wheeler, Eds.) Encyclopedia of Biotechnology in Agriculture and Food. Marcel Dekker, New York, NY (in press)


2. BOOKS, NON-REFEREED BOOK CHAPTERS, PROCEEDINGS, INSTRUCTIONAL MEDIA, THESES/DISSERTATIONS

Ahola, J. K., Aznarez, V. A., Seidel, G. E. Jr., and Whittier, J. C. 2006. Superimposing 14d MGA pre-feeding and(or) 7d CIDR on the SelectSynch (GnRH-PG) estrus synchronization protocol in beef cows. Proc. Western Sect. Am. Soc. Anim. Sci. 57:272-275.

Curchoe C. 2006. Epigenetics of bovine clones. PhD thesis. University of Connecticut

De La Torre-Sanchez, J. F., and Seidel, G. E. Jr. 2006. Evolucion de los medios para culturo de embriones bovinos. In: Memorias del XI Curso Internacional de Reproduccion Bovina, FMVZ-UNAM, Mexico, D.F., pp. 104-121.

Farin PW. 2006. Estrus synchronization programs for cows and heifers. Proc 142nd Annual Convention, AVMA, July 15-19, 2006, Honolulu

Farin PW. 2006. Management of embryo donor cows and recipients. Proc 142nd Annual Convention, AVMA, July 15-19, 2006, Honolulu.

Farin PW. 2006. Reducing fetal loss in cattle. Proc 142nd Annual Convention, AVMA, July 15-19, 2006, Honolulu.

Farin PW. 2006. Update on sexed semen, ivf and cloning in cattle. Proc 142nd Annual Convention, AVMA, July 15-19, 2006, Honolulu.

Guerrerro, C. 2006. Cryopreservation and Intracytoplasmic with Bovine Epididymal Spermatozoa. PhD Dissertation, Louisiana State University. August, 2006.

Jiang L. 2006. Reprogramming of epigenetically-regulated genes in cloned pigs. PhD thesis. University of Connecticut.

Leed, A. 2006. Characterization of potential porcine oocyte plasma membrane receptors. M.S. thesis, University of California-Davis.

Moisan, A. 2006. Vitrification and Dehydration for the Preservation of Gametes.
PhD Dissertation Louisiana State University. December, 2006.

Nel-Themaat, L. 2006. Gamete and Cell Technolgies for Use in Biological Resource Banking. PhD Dissertation, Louisiana State University. May, 2006.

Pinisetty, D. J. Saenz, R.A. Godke and R.V. Devereddy. 2006. Theoretical predictions of optimal cooling rates for cryopreservation of caprine sperm. Proc. ASME Bioengineering Meetings. CD Publication 2006.

Preis, K. 2006. Metabolic Characteristics and Gene Expression of In Vitro and In Vivo Matured Mammalian Oocytes. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.

Seidel, G. E., Jr. 2006. On the usefulness of an update on assisted reproductive technologies in cattle. Theriogenology 65:1-3.

Seidel, G. E., Jr., and Schenk, J. L. 2006. Sex-selected semen. Proc. Applied Reproductive Strategies in Beef Cattle. Univ. Missouri, Columbia, MO, pp. 315-321.

Seidel, G. E., Jr., and Wheeler, M. B.. 2006. Cloning Pets. In: Proc. Symposium on Cloning Pets, M.B. Wheeler and G.E. Seidel, Jr. (eds), pp. 1-8.

Sendemir-Urkmez, A., S.G. Clark, M.S. Goldwasser, M.B. Wheeler, R.D. Jamison. 2006. Tissue Engineered Bone for Mandibular Reconstruction. American Association of Oral-Maxiofacial Surgeons Annual Meeting, October 2006, San Diego, CA pgs. 42-43.

Suh, T. K., Stokes, J., Squires, E. L., and Seidel, G. E. Jr. 2006. Effect of removing acrosomes from stallion sperm before ICSI of bovine oocytes. Anim. Reprod. Sci. 303-306.

Wagoner Johnson, A. J., Woodard, J., Hilldore, A., Jamison, R.D., Wheeler, M.B., Clark, S.G. 2006. Bone-Like Behavior of Brittle, porous Hydroxyapatite Implants With Microporosity. American Association of Oral-Maxiofacial Surgeons Annual Meeting, October 2006, San Diego, CA pgs. 40-41.

Wheeler, M.B. 2006. Cloning and Transgenic Animals for Agriculture. 2nd International Symposium of Applied Animal Reproduction, Londrina, Parana, Brazil, October 5-7, 2006. Pgs. 1-18.

White, K.L., G.L. Woods, D.K. Vanderwall, G. Li, and T.D. Bunch. 2006. Cloning the Equine. Epigenetic Risks of Cloning. CRC Press, Taylor & Francis Group, LLC. pp 59 - 69.

Wilson, M. 2006. Bovine and Equine Recombinant Follicle Stimulating Hormone Produced in the Heterologous Host Trypansoma brucei. M.S. Thesis, Clemson University, Clemson, SC.

Youngs, C.R. and A.E. Freeman. 2006. Breeding (Animal). In McGraw-Hill Encyclopedia of Science and Technology, 10th Ed. (in press).



3. ABSTRACTS

Barcelo-Fimbres, M., Brink, Z., and Seidel, G. E. Jr. 2006. Effects of PES during in vitro culture of bovine embryos on pregnancy rates. J. Anim. Sci. 84 (Suppl. 1):432.

Barcelo-Fimbres, M., and Seidel, G. E. Jr. 2006. Effects of fetal calf serum and fructose or glucose on embryonic development and lipid accumulation of bovine embryos. Reprod. Fertil. Develop. 18:186.

Berger, T. and A. Leed. 2006. Putative molecules involved in porcine sperm-oocyte plasma membrane interaction 10th International Symposium on Spermatology Madrid.

Burns, M., D. Lapin, A. Brown, J. Gibbons. 2006. Effects of progesterone and presence of a corpus luteum on superovulation in beef cattle. 32nd Annual Conference of the International Embryo Transfer Society, Orlando, FL. Abstract 360.

Burns, M., D. Lapin, A. Brown, J. Gibbons. 2006. Effects of progesterone and presence of a corpus luteum on superovulation in beef cattle. 32nd Annual Conference of the International Embryo Transfer Society, Orlando, FL. Abstract 360.

Campos-Chillon, L. F., Cox, J., Seidel, G. E. Jr., and Carnevale, E. M. 2006. Vitrification of large equine embryos. Reprod. Fertil. Develop. 18:151.

Campos-Chillon, L. F., Suh, T. K., Seidel, G. E. Jr., and Carnevale, E. M. 2006. Use of bovine embryos to establish methods for vitrification of early equine embryos. Theriogenology 66:684-685.

Chang CC, Nagy ZP, Elliott TA, Wright G, Kort HI, Yang X, Tian XC. The DNA methylation and histone acetylation of oocytes in juvenile and adult mice. Hum Reprod 2006; 21(S1):i14. (presented in the European Society of Human Reproduction and Embryology annual meeting, Prague).

Chang CC, Nagy ZP, Kort HI, Rasmussen TP, Yang X, Tian XC. Dynamic distribution of heterochromatin protein 1b in the preimplantation embryos. Fertil Steril 2006; 86(2S):S79-80. (New Orleans, American Society for Reproductive Medicine in 2006 annual meeting).

Chang CC, Nagy ZP, Shapiro DB, Mitchell-Leef D, Yang X, Tian XC. Dynamic distribution of g-tubulin during mouse oocyte maturation. Fertil Steril 2006; 86(2S):S96-97. (New Orleans, American Society for Reproductive Medicine in 2006 annual meeting).

Curry, E., S. Ellis, D. Lapin, and J. Gibbons. 2006. Efficacy determination of HEIFERPlusÔ semen sexing kit in superovulated cows. Society for the Study of Reproduction Meetings, Omaha, NE. Abstract 499.

Davidson, T.R., S.A. Malusky, C.E. Ferguson, S.J. Lane, M.B.Wheeler, W.L. Hurley. 2006. Isolation of multilineage stem cells from the porcine mammary fat pad. Reproduction, Fertility and Development 18:171.

Du,F., J Xu, S Gao, L-Y Sung, D Stone, M Joyner, J Zhang, S Chaubal, X Tian, YE Chen, X Yang. Full-term and live rabbit clones produced by somatic cell nuclear transfer. Reprod Fert Dev 2006; 18:124 (presented at the annual meeting of the International Embryo Transfer Society, Florida, 2006).

Estrada J, Lee E, and Piedrahita J. Cryopreservation of donor cells for nuclear transfer: effect of cell freezing method on the efficiency of somatic cell nuclear transfer in pigs. Reprod Fert Dev 18:125 (abst 32), 2006.

Everts RE, Razzak A, Chavatte-Palmer P, Hue I, Green CA, Oliveira R, Rodriguez-Zas S, Tian XC, Yang X, Renard JP, Lewin HA. 2006. Gene expression profiling of placentomes from cloned cattle. A model for mammalian systems biology. (to be presented in "Pathways, Networks, and Systems" to be held in Mykonos, Greece, October 8-13).

Everts, RE, A Razzak, P Chavatte-Palmer, I Hue, CA Green, R Oliveira, S Rodriguez-Zas, XC Tian, X Yang, J-P Renard, HA Lewin. 2006. Global gene expression profiling distinguishes placentomes derived by artificial insemination from those pregnancies derived by either in vitro fertilization or nuclear transfer. PAG abstract guide: p282. Presented at the Plant & Animal Genome XIV Conference, January 14 - 18, 2006, San Diego, California

Everts RE, Sommers A, Green CA, Oliveira R, Rodriguez-Zas S, Sung LY, Du F, Evans ACO, Boland M, Fair T, Lonergan P, Renard JP, Yang X, Lewin HA, XC Tian. Major differences in Gene expression profiles revealed in Day-25 placental tissues collected from Cows carrying cloned fetuses (to be presented in the Plant & Animal Genome XV Conference, January 13-17, 2007, San Diego, USA).

Farin CE, Sommer JR, Rodriguez KF, Petters RM, and Alexander JE. Functional analysis using short-interfering (si)RNA of TRAM-6, a novel transcript associated with oocyte maturation in cattle Reprod Fert Dev 18: 269-270 (abst 324), 2006.

Giraldo, A.M., J.A. Jenkins, J.W. Lynn, R.A. Godke and K.R. Bondioli. 2006. Histone phosphorylation patterns and chromosomal stability of cultured bovine fibroblasts. Reprod. Fertil. Develop. 18:292.

Guerrero, C.A., S.P. Leibo, K.R. Bondioli and R.A. Godke. 2006. Effect of cryoprotective agent addition and removal at 4°C on motility and plasma membrane integrity of bovine caudal epididymal spermatozoa. Reprod. Fertil. Develop. 18:156

Hartke J.L., J.M. Drnevich, M.B. Wheeler, S.M. Donovan. 2006. Transgenic Overexpression of Insulin-Like Growth Factor-I (IGF-I) in Milk Influences Intestinal Development and Gene Expression in Piglets. 13th Int.Conference Int. Soc. Research Human Milk and Lactation, Niagara-on-the-Lake, Ontario, Canada, September 22-26, 2006.

Jiang L, Prather RS, Jobst P, Ayares D, Yang X, Tian XC. Expression of growth-related imprinted genes in decreased newborn and surviving cloned piglets. Reprod Fert Dev 2006; 18:232 (presented at the annual meeting of the International Embryo Transfer Society, Florida, 2006).

Kaya A, Schindler J, Memili E and Parrish JJ. (2006) Melatonin improves bovine embryo production and protects embryos from heat stress in vitro. Abstract # 398. Biol Reprod Special Issue.

Kim C, Ma Y, Chang C-C, Yang X, Rasmussen T, Tian XC. The Typical Pattern of Histone MacroH2A1 in Preimplantation Bovine Embryos Following Activation and In Vitro Fertilization. Reprod Fert Dev 2006; 18:232-233 (presented at the annual meeting of the International Embryo Transfer Society, Florida, 2006).

Krisher, R.L. (2005) Inhibition of the pentose phosphate pathway results in meiotic arrest in porcine oocytes that can be overcome by the addition of pathway cofactors and end products. Reprod. Fertil. Dev. 17:293 (abstr. 287).

Krisher, R., Auer, A., Clark, K., Emsweller, K., Rogers, S., Thomas, K., Chatiza, F., Bartels, P. (2007) In vitro production of springbok (Antidorcas marsupialis) embryos. Reprod. Fertil. Dev.(in press).

Krisher, R., Wulster-Radcliffe, M., Spurlock, M., Sturek, M. (2006) The Ossabaw Pig as a Model of Polycystic Ovary Syndrome. Experimental Biology; abst. 490.3.

Kubota C, Kojima T, Nagai T, Tian X, Yang X. 2007. BOVINE EMBRYO DEVELOPMENT AFTER IVM/IVF/IVC OF OOCYTES STORED FOR 22H IN VARIOUS MEDIA (to be presented in the annual meeting of IETS in Kyoto Japan).

Lima, A.S., S.A. Malusky, M.R.B. Mello, S.J. Lane,, J.R. Rivera, M.B.Wheeler. 2006.Transplantation and in vivo differentiation of adipose-derived stem cells in swine. Reproduction, Fertility and Development 18:208.

McAllister, S. Bushnell, M. Purpera, A. Landry, B. Olcott, H. Blackburn, E. Loetz, K. Bondioli and R.A. Godke. 2006. Collection and freezing of Angora goat embryos for the USDA animal germplasm repository. Southern Section, J. Anim. Sci. (abstract).

Memili E, Peddinti D, Sagirkaya H and Burgess SC. (2006) Descriptive proteomics of bovine oocytes and cumulus cells. Abstract # 358. Biol Reprod Special Issue.

Misirlioglu M, Page GP, Sagirkaya H, Kaya A, Parrish JJ, First NL and Memili E. (2006) Embryonic genome activation in bovine. Presented at the Gordon Research Conference on Mammalian Gametogenesis and Embryogenesis, New London, CT, June 17-23, 2006.

Moisan, A.E., S.P. Leibo, J.W. Lynn, M.C. Gómez, C. E. Pope, K Bondioli, L. Dresser, R. A. Godke. 2006. Blastocyst development from felid oocytes injected with dehydrated spermatozoa. Reprod. Fertil. Develop. 18:285.

Moisan, A., S.P. Leibo, J.W. Lynn, M.C. Gomez, C.E. Pope, K. Bondioli, B.L. Dresser and R.A. Godke. 2006. Development of felid oocytes injected with freeze-dried or air-dried spermatozoa. Cryobiology (abstract).

Nel-Themaat, L., M.C. Gomez, G. Wirtu, A. Cole, K.R. Bondioli, B.L. Dresser, C.E. Pope and R.A. Godke. 2006. Imprvement in semen derived epithelial cells proliferated by fibroblast co-culture. Reprod. Fertil. Develop. 18:140.

Paczkowski, M., Terry, D., Bidwell, C., Krisher, R. (2006) Differential Gene and Protein Expression in Gilt and Sow Derived Oocytes. Proceedings, Midwestern ASAS.

Paczkowski, M., Terry, D., Krisher, R. (2005) Differential Protein Expression of In Vitro Matured Porcine Oocytes Derived from Gilts and Sows. Biology of Reproduction Suppl. Biol. Reprod. Special Issue abstr. 526:197.

Pant, D., Keefer, C.L. 2006. Gene expression of inner cell masses isolated from in vitro produced and in vivo derived bovine blastocysts. Reprod Fert Dev 18(2):110, abst. 4.

Peláez, J. and Long J. 2006. Storage of turkey semen at 4°C for 24h alters the sperm glycocalyx. Poultry Science Meeting, Edmonton, Canada, July 2006.

Pelaez, J. and Long, J. 2006. Flow cytometry analysis of the turkey sperm glycocalyx: effects of neuraminidase treatment. International Embryo Transfer Society Meeting, Orlando, FL, January 2006.

Piedrahita J, Bischoff S, Estrada J, Freking B, Nonneman D, Martin A, Mir B, Rohrer G, and Tsai S. Use of porcine parthenotes and gene expression profiling using microarrays for identification of imprinted genes. Reprod Fert Dev 18:239 (abst 253), 2006

Preis, K. A., Seidel, G. E. Jr., and Gardner, D. K. 2006. Oxygen tension and EGF affect metabolism of in vitro matured cumulus-enclosed mouse oocytes. Reprod. Fertil. Develop. 18:278.

Preis, K. A., Seidel G., and Gardner, D. K. 2006. Maturation conditions affect metabolism and developmental competence of in vitro matured cumulus-enclosed oocytes. Proc. Ann. Mtg .Am. Soc. Reprod. Health. In Press.

Purcell, S. H., and Seidel, G. E. Jr. 2006. Timing of addition of cryoprotectant for vitrification of morula stage bovine embryos. Biol. Reprod. (Special Issue):90.

Purcell, S. H., Suh, T. K., and Seidel, G. E. Jr. 2006. Oocyte aspiration from transitional and cycling mares treated with eFSH. Reprod. Fertil. Develop. 18:291.

Reeder, A., V. Schutzkus, J. Wiebelhaus-Finger, H. Khatib, R. Monson, M.B. Wheeler, D.J. Beebe, J.J. Rutledge. 2006. Assesment of hsp70.1 transcription levels in bovine embryos after cumulus removal by different techniques. Reproduction, Fertility and Development 18:111.

Roberts, M. and T. Berger. 2006. Role for estrogen in development of porcine accessory sex glands. 39th Annual meeting of the Society for the Study of Reproduction. Omaha, NE.

Rodriguez N, Kim IJ, Jung S, Kaya A and Memili E. (2006) Effect of Melatonin on Development of Porcine Preimplantation Embryos in vitro. Molecular Biology of the Cell. December 2006 special issue.

Rodriguez N, Wang Z, Kasinathan S, Page G and Memili E. (2006). Transcriptional Reprogramming of Gene Expression in Bovine Somatic Cell Nuclear Transfer Embryos. Molecular Biology of the Cell. December 2006 special issue.

Smith SL, Sung L-Y, Page R, Henderson B, Du F, Everts R, Nedambale T, Rodriguez-Zas S, Renard J-P, Lewin H, Yang X, Tian XC. Global Gene Expression Profiling of Single Bovine Embryos Reveals Significant Effects of In Vitro Maturation, Fertilization and Culture on Gene Expression. Reprod Fert Dev 2006; 18:111 (presented at the annual meeting of the International Embryo Transfer Society, Florida, 2006).

Suh, T. K., Purcell, S. H., and Seidel, G. E. Jr. 2006. ICSI and activation of oocytes recovered from transitional and cyclic mares. Reprod. Fertil. Develop. 18:286-287.

Taylor, P.J., S.A. Taylor, M. Sansinena and R.A. Godke. 2006. Using a novel coaxial microinjection system and embryo vitrification to produce llama (Lama glama) pregnancies: A preliminary study. Reprod. Fertil. Develop.18:164.

Tian XC, Kubota C, Yang X. 2006. Meat and Milk composition of bovine clones. Transgenic Research 15:121. Presented in the 2005 UC Davis Transgenic Animal Research Conference V. Aug 12-16, 2006. Tahoe City, CA.

Tubman, L., Peter, A., Krisher, R.L. (2005) Effect of energy substrates on metabolism and meiosis of porcine oocytes during in vitro maturation. Reprod. Fertil. Dev. 17:301 (abstr. 301).

Tubman, L., Peter, A., Krisher, R.L. (2006) Pentose phosphate pathway activity controls nuclear maturation of porcine oocytes. Reprod. Fertil. Dev. 18:279 (abst 344).

VanEtten, T., F.D. Ireland, D. Vandever, D.J. Kesler, M.B. Wheeler. 2006. Evaluation of a novel electronic estrus detection device in recipients synchronized for embryo transfer. Reproduction, Fertility and Development 18:204-205.

Wagoner Johnson, A. J., J. Woodard, A. Hilldore, C. Park, S. Lan, R.D. Jamison, M. B. Wheeler, S. Clark. 2006. A Transition from Ceramic-like to Cancellous Bone-like Behavior in Porous Hydroxyapatite Scaffolds," Society for Physical Regulation in Biology and Medicine, Cancun, Mexico, January, 2006.

Walker, D. J., and Seidel, G. E. Jr. 2006. Vitrification of bovine embryos in medium with polyvinyl alcohol replacing BSA. Reprod. Fertil. Develop. 18:165.

Wilson, M., J. Morris, and J. Gibbons. 2006. Bovine and equine recombinant Follicle Stimulating Hormone produced in two heterologous hosts: Escherichia coli and Trypanosoma brucei. 32nd Annual Conference of the International Embryo Transfer Society, Orlando, FL. Abstract 268.

Wirtu G, C.E. Pope, A. Cole, R.A. Godke, D.L. Paccamonti and B. L. Dresser. 2006. Sperm cryopreservation in tragelaphine antelopes. Reprod. Fertil. Develop.18:166.

Youngs, C.R. 2006. Development of a course in embryo transfer and related technologies for undergraduate students in agriculture. J. Anim. Sci. 84(Suppl. 1):381.



4. MISCELLANEOUS PUBLICATIONS(SEMI-TECHNICAL/LAY PUBLICATIONS)

Amann, R. P., and Seidel, G. E. Jr. 2006. Give sexed semen some time. Hoards Dairyman 15:636.

Ballard, C., J. Smith, J. Glaser, K.R. Bondioli and R.A. Godke. 2006. Pre-breeding characteristics and pregnancy rates in crossbred beef heifers. LA Beef Cattle/ Forage Res. Rep. (In press)

Farin CE, and Farin PW. Reproductive technologies&are they a fit for your operation? Carolina Cattlemans Connection (2006, in press).

Gentry, Jr. G.T., B.M. Olcott , K.R Bondioli and R. A. Godke. 2006. Estrus synchronization and artificial insemination of Brahman x Hereford crossbred cattle. LA Beef Cattle/ Forage Res. Rep. (In press)

VanEtten, T.L. and Wheeler, M.B. 2006. Evaluation of a novel electronic estrus detection device. Illinois Dairy Report 74-76.

Wheeler, M.B. 2006.The use of gender-selected semen in dairy production schemes. Illinois Dairy Report 72-73.

05/01/2008

1. REFEREED ARTICLES/BOOK CHAPTERS

At-Taras, E. E., I.C. Kim, T. Berger, A. Conley, and J.F. Roser. 2008. Reducing endogenous estrogen during development alters hormone production by porcine leydig cells and seminiferous tubules. Domest Anim Endocrinol 34:100-108.

Barcelo-Fimbres, M. and G.E. Seidel, Jr. 2007. Effects of fetal calf serum, phenazine ethosulfate and either glucose or fructose during in vitro culture of bovine embryos on embryonic development after cryopreservation. Mol Reprod Dev 74:1395-1405.

Barcelo-Fimbres, M. and G.E. Seidel, Jr. 2007. Effects of either glucose or fructose and metabolic regulators on bovine embryonic development and lipid accumulation in vitro. Mol Reprod Dev 74:1406-1418.

Berger, T., M. McCarthy, C. A. Pearl, E. At-Taras, J. F. Roser, and A. Conley. 2007. Reducing endogenous estrogens during the neonatal and juvenile periods affects reproductive tract development and sperm production in postpuberal boars. Anim Reprod Sci.

Berger, T., and B. M. Roberts. Reduced immunolabeling of a porcine oocyte membrane protein reflects reduced fertilizability of porcine oocytes following elevated ambient temperature. Reprod. Domestic Animals doi: 10.1111/j.1439-0531.2007.01052.

Bleck, G.T., M.B. Wheeler, L.B. Hansen, H. Chester-Jones, and D.J. Miller. (2007) Lactose synthase components in milk: Concentrations of ±-lactalbumin and ²1,4-galactosyltransferase in milk of cows from several breeds at various stages of lactation. Reprod. Dom. Anim. doi: 10.1111/j.1439-0531.2007.01047.xISSN 0936-6768 (In Press).

Checura, C.M. and G.E. Seidel, Jr. 2007. Effect of macromolecules in solutions for vitrification of mature bovine oocytes. Theriogenology 67:919-930.

Corbin, C. J., A. L. Hughes, J. R. Heffelfinger, T. Berger, T. B. Waltzek, J. F. Roser, T. C. Santos, M. A. Miglino, M. F. Oliveira, F. C. Braga, F. V. Meirelles, and A. J. Conley. 2007. Evolution of suiform aromatases: Ancestral duplication with conservation of tissue-specific expression in the collared peccary (pecari tayassu). J Mol Evol 65: 403-412.

Estrada, J., J. Sommer, B. Collins, B. Mir, A. Martin, A. York, R.M. Petters, and J.A. Piedrahita. 2007. Swine generated by somatic cell nuclear transfer have increased incidence of intra uterine growth restriction (IUGR). Cloning and Stem Cells 9:229-236.

Everts, R.E., P. Chavatte-Palmer, A. Razzak, I. Hue, C.A. Green, R. Oliveira, X. Vignon, S.L. Rodriguez-Zas, X.C. Tian, X. Yang, J.P. Renard, and H.A. Lewin. 2008. Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer. Physiol Genomics (In Press).

Farin, C.E., K.F. Rodriguez,, J.E. Alexander, J.E. Hockney, J.R. Herrick, and S. Kennedy-Stoskopf. 2007. The role of transcription in EGF- and FSH-mediated oocyte maturation in vitro. Anim Reprod Sci 98:97-112.

Farin, P.W., K. Moore, and M. Drost. Assisted reproductive technologies in cattle. In: Current Therapy in Large Animal Theriogenology, 2nd Ed., RS Youngquist and WR Threlfall (Editors) , Elsevier, Inc., 2007; 496-508.

Feugang, J.M., O.D. Camargo-Rodriguez, and E. Memili. 2008. Culture Systems for Bovine Embryos. Livestock Science (In Press).

Flores, R., M. L. Looper, R. W. Rorie, D. M. Hallford, and C. F. Rosenkrans Jr. 2007. Endocrine and follicular dynamics are influenced by body condition and somatotropin in postpartum beef cows. J. Anim. Sci. (Accepted).

Flores, R., M. L. Looper, R. W. Rorie, M. A. Lamb, S. T. Reiter, D. M. Hallford, D. L. Kreider, and C. F. Rosenkrans. 2007. Influence of body condition and bovine somatotropin on estrous behavior, reproductive performance, and concentrations of serum somatotropin and plasma fatty acids in postpartum Brahman-influenced beef cows. J. Anim. Sci. 85:13181329.

Herrick, J.R., J.B. Bond, G.M. Magarey, H.L. Bateman, R.L. Krisher, S.A. Dunford, and W.F. Swanson. 2007. Toward a Feline-Optimized Culture Medium: Impact of Ions, Carbohydrates, Essential Amino Acids, Vitamins, and Serum on Development and Metabolism of In Vitro Fertilization-Derived Feline Embryos Relative to Embryos Grown In Vivo. Biol. Reprod. 76:858-870.

Jiang, L., P. Jobst, L. Lai, M. Samuel, D. Ayares, R.S. Prather, and X.C. Tian. 2007. Expression of Growth-Regulating Imprinted Genes in Cloned Piglets. Cloning Stem Cell 9:97-106.

Jiang, L, L. Lai, M. Samuel, R.S. Prather, X. Yang, and X.C. Tian. 2007. Expression of X-linked Genes in Deceased Neonates and Surviving Cloned Female Piglets. Mol Reprod Dev DOI: 10.1002/mrd.20758.

Keefer, C.L., D. Pant., L. Blomberg, and N.C. Talbot. 2007. Challenges and prospects for the establishment of embryonic stem cell lines of domesticated ungulates. Anim Reprod Sci 98:147-168.

Krisher, R.L., A.M. Brad, J.R. Herrick, M.L. Sparman, and J.E. Swain. 2007. A comparative analysis of metabolism and viability in porcine oocytes during in vitro maturation. Anim Reprod Sci 98:72-96.

Lee. E., J. Estrada and J.A. Piedrahita, 2007. A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methods. Animal Biotechnology (In Press).

Li C., Y.F. Bin, X.C. Tian,C. Curchoe, L. Yang, D.Y. Feng, Q.Y. Jiang, M. ONeill, and S.Q. Zhang. 2008. Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa). Animal Biotechnology (In Press).

Liu, Y., G.P. Li, L.F. Rickords, K.L. White, B.R. Sessions, K.I. Aston, and T.D. Bunch. (2007) Effect of nicotine on in vitro maturation of bovine oocytes.. Anim Reprod Sci (In Press).

Liu, Y., G.P. Li, K.L. White, L.F. Rickords, B.R. Sessions, K.I. Aston, and T.D. Bunch. (2007) Nicotine Alters Bovine Oocyte Meiosis and Affects Subsequent Embryonic Development. Mol Reprod Dev 74:1473-1482.

Memili, E., D. Peddinti, L.A. Shack, B. Nanduri, F. McCarthy, H. Sagirkaya, and S.C. Burgess. 2007. Global proteome of bovine germinal vesicle oocytes and cumulus cells. Reproduction 133:1107-20.

Miles, J.R., L.A. Blomberg, R.L. Krisher, R.E. Everts, T.S. Sonstegard, R.L. Van Tassell, and K.A. Zuelke. 2007. Small amplified RNA-serial analysis of gene expression (SAR-SAGE) from porcine blastocysts produced in vivo or in vitro. Mol.Reprod Dev. (In Press).

Niemann, H., X.C. Tian, W.A. King, and, R. Lee. 2007. Epigenetic reprogramming in bovine embryonic and foetal development upon somatic cell nuclear transfer cloning. Reproduction (In Press).

Paris, M.C.J., G.F. Mastromonaco, D.B.B.P. Paris, and R.L. Krisher. 2007. A perspective on the role of emerging technologies for the propogation of companion animals, non-domestic and endangered species. Reprod Ferti. Dev 19:iii-vii.

Pate, B.J., K.L. White, D.B. DeWald, L.F. Rickords, G. Li, K.I. Aston, B. Sessions, and T.D. Bunch. (2007) Involvement of tyrosine kinases, specifically Src family kinases, focal adhesion kinase (FAK) and agonist-induced PLC in the activation and development of bovine oocytes. Reproduction. (In Press).

Pate, B.J., K.L. White, D. Chen, K.I. Aston, B.R. Sessions, T.D. Bunch, and B.C. Weimer. (2007) A novel approach to identify bovine sperm membrane proteins that interact with receptors on the vitelline membrane of bovine oocytes. Mol Reprod Dev (Published online September 20, 2007).

Pate, B.J., K.L. White, Q.A.Winger, L.F., Rickords, K.I. Aston, B.R. Sessions, G.P.Li, K.D. Campbell, B. Weimer, and T. Bunch. (2007) Specific Integrin Subunits In Bovine Oocytes, Including Novel Sequences For Alpha 6 And Beta 3 Subunits.. Mole Reprod Dev 74:600-607.

Pearl, C. A., E. At-Taras, T. Berger, and J. F. Roser. 2007a. Reduced endogenous estrogen delays epididymal development but has no effect on efferent duct morphology in boars. Reproduction 134: 593-604.

Pearl, C. A., T. Berger and J. F. Roser. 2007b. Estrogen and androgen receptor expression in relation to steroid concentrations in the adult boar epididymis. Domest Anim Endocrinol 33:451-459.

Peddinti, D., B. Nanduri, A. Kaya, J.M. Feugang, S.C. Burgess, and E. Memili. 2008. Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. In press. BMC Systems Biology.

Powell, S. A., B. B. Smith, K. I. Timm, and A. R. Menino, Jr. 2007. Estradiol production by preimplantation blastocysts and increased serum progesterone following estradiol treatment in llamas. Anim Reprod Sci 102:66-75.

Powell, S. A., B. B. Smith, K. I. Timm, and A. R. Menino, Jr. 2007. Expression of estrogen receptors ± and ² in the corpus luteum and uterus from non-pregnant and pregnant llamas. Mol Reprod Dev.74:1043-1052.

Preis, K.A., G.E. Seidel, Jr., and D.K. Gardner. 2007. Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation. Mol Reprod Dev 74:893-903.

Ramesh, R., C. A. Pearl, E. At-Taras, J. F. Roser, and T. Berger. 2007. Ontogeny of androgen and estrogen receptor expression in porcine testis: Effect of reducing testicular estrogen synthesis. Anim Reprod Sci 102 286-299.

Rodriguez-Osorio, N, Z. Wang, P. Kasinathan, G.P. Page, J.M. Robl, and E. Memili. 2008. Transcriptional Reprogramming of Gene Expression in Bovine Somatic Cell Chromatin Transfer Embryos. (Submitted).

Rodriguez-Osorio, N., I.J. Kim, H. Wang, A. Kaya, and E. Memili.. 2007. Melatonin increases cleavage rate of porcine preimplantation embryos in vitro. J Pineal Res 43:283-288.

Sagirkaya, H., M. Misirlioglu, H. Kaya, J.J. Parrish, N.L. First, and E. Memili. 2007. Developmental Potential of Bovine Oocytes Cultured in Defined Maturation Medium. Anim Reprod Sci 101:225-240.

Seidel, G.E., Jr. 2007. Overview of sexing sperm. Theriogenology 68:443-446.

Stevenson, J.S., D.E. Tenhouse, R.L. Krisher, G.C. Lamb, J.E. Larson, C.R. Dahlen, J.R. Pursley, N.M. Bello, P.M. Fricke, M.C. Wiltbank, D.J. Brusveen, M. Burkhart, R.S. Youngquist, and H.A. Garverick. 2008. Detection of Anovulation by Heatmount Detectors and Transrectal Ultrasonography before Treatment with Progesterone in a Timed Insemination Protocol. J Dairy Sci (Accepted).

Sung, L.Y.,P.C. Shen, B.S. Jeong, J. Xu, C.C. Chang, W.T.Cheng, J.S.Wu, S.N. Lee, D. Broek, D. Faber, X.C. Tian,, X. Yang, and F. Du. 2007. Premature Chromosome Condensation Is Not Essential for Nuclear Reprogramming in Bovine Somatic Cell Nuclear Transfer. Biol Reprod 76:232-240.

Swain, J.E., C.L. Borman, A.M. Brad, R.E. Durkin, and R.L. Krisher. 2008. Metabolism of glucose, pyruvate and glutamine by porcine oocytes matured in vitro and in vivo. Mol Reprod Dev (Submitted).

Tanphaichitr, N., E. Carmona, M. Bou Khalil, H. Xu, T. Berger, and G. L. Gerton. 2007. New insights into sperm-zona pellucida interaction: Involvement of sperm lipid rafts. Front Biosci 12:1748-1766.

Tsai, S., J. Cassady, D. Freking, G. Nonneman, G. Rohrer,and J.A. Piedrahita. 2006. Annotation of Affymetrix Porcine genome Microarray Chip. Animal Genetics 37:423-424.

Tsai, S., B. Mir, A.C. Martin, J.L. Estrada, S.R. Bischoff, W.P. Hsieh, J.P. Cassady, B.A. Freking, D.J. Nonneman, G.A. Rohrer, and J.A. Piedrahita. 2006. Detection of transcriptional difference of porcine imprinted genes using different microarray platforms. BMC Genomics. 7:328-336.

Whang, H.S., M.A. Hunt, N. Wrench, J.E. Hockney, C.E. .Farin, and A. Tonelli. 2007. Nonoxynol-9-alpha-cyclodextrin inclusion compound and its application for the controlled release of nonoxynol-9 spermicide. J Applied Polymer Sci 106:4104-4109.

Wheeler, M.B., E.M. Walters, and D. J. Beebe. 2007. Toward Culture of Single Gametes: The Development of Microfluidic Platforms for Assisted Reproduction. Theriogenology 68S:S178-S189.

Wheeler, M.B. 2007. Agricultural applications for transgenic livestock. Trends in Biotechnology 25:204- 210.

Woodard, J., A. Hilldore, M. B. Wheeler, S. Clark, S. Lan, R. Jamison, and A. J. Wagoner Johnson. 2007. The mechanical properties and osteoconductivity of hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28:4554.

Wrench, N., C.R.F. Pinto, G. Klinefelter, and C.E. Farin. Seasonal expression and pattern of S22 immunolocalization in stallion semen. Anim Reprod Sci 94:32-35, 2006.

Yang, X., X.C. Tian, C. Kubato, R. Page, J. Xu, J. Cibelli, and G.E. Seidel, Jr. 2007. Risk assessment of meat from cloned animals. Nature Biotechnol 25:77-83.

Yang X, X.C. Tian, C. Kubota, R. Page, J. Xu , J. Cibelli , and G. Seidel, Jr. 2007. Risk assessment of meat and milk from cloned animals. Nature Biotechnol 25:79-83.

Yang X, S.L. Smith, X.C. Tian, H.A. Lewin, J.P. Renard, and T. Wakayama. 2007. Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning. Nature Gen 39:295-302.

Yang X, and S. Smith. 2007. ES cells derived from cloned embryos in monkey  a jump toward human therapeutic cloning. Cell Res 17:969-970.

Yang X, and M. Carter. 2007. Transgenic animal bioreactors: A new line of defense against chemical weapons. Proc Natl Acad Sci USA 104:13859-13860.

Zaunbrecher, G., B. Mir, P.W. Dunne, M. Breen, and J.A. Piedrahita. 2007. Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ). Animal Biotechnology. (In Press).

2. BOOKS, NON-REFEREED BOOK CHAPTERS, PROCEEDINGS, INSTRUCTIONAL MEDIA, THESES/DISSERTATIONS

Barcelo-Fimbres. 2007. Energy Substrates, Metabolic Regulators and Lipid Accumulation During the Culture of In Vitro-Produced Bovine Embryos. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.

Hockney, J.E. Candidate mRNAs regulating meiotic resumption in bovine cumulus-oocyte-complexes. MS Thesis, North Carolina State University. 2007.

Memili, E. 2007. Advances in animal cloning in the era of functional genomics and beyond. The 7th International Symposium on Developmental Biotechnology. Jeju University, Jeju, Korea, October 26-27, 2007.

Memili, E. 2007. Advances and applications of genomics and proteomics in gamete and embryo science. XXI. Brazilian Embryo Technology Conference in Costa do Sauipe, BA, Brazil, August 23-27, 2007.

Seidel, G.E., Jr. 2007. Sexing sperm. Proc. XII Congreso Latinoamericano de Buiatria, pp. 3-7.

Seidel, G.E., Jr. 2007. New technologies for reproduction in cattle. Proc. Symposium Applied Reproductive Technologies in Beef Cattle, pp. 285-291 and 7th Pre-conference, Strategies Internacional de Actualizacion Veterinaria, 7 pp.

Seidel, G.E., Jr. 2007. New methods of cryopreserving bovine embryos. Proc. 26th Annual Conference, American Embryo Transfer Association, 5 pp.

Smith, S. 2007. Expression profiling of cloned embryos and donor cells. PhD Dissertation.

Wheeler, M.B., D. Kim, E. Monaco, and W.L. Hurley. 2007. Application of Robotic and Microfluidic Technologies to Stem Cell Culture and Analysis. Tsukuba Meeting for Animal Biotechnology (TMAB)  Progress in Somatic Cell Nuclear Transfer Technology, January 12-12, 2007 Tsukuba City, Japan, Pgs. 31-32.

Wheeler, M.B., T.R. Davidson, S.A. Malusky, C.E. Ferguson, A.S. Lima, and M.B. Mello. 2007. PRODUCTION OF TRANSGENIC LIVESTOCK: Technology and Applications. In: Biotécnicas Aplicadas à Reprodução Animal, eds. P.B. Goncalves & J. Figuriedo, (New Edition In Press).

Wheeler, M.B., M.R.B. Mello, A.S. Lima, and T. VanEtten. 2007. Modification of the Domestic Animal Genome. In Bovine Theriogenology, de la Sota, R.L. and Bo, G.A., Eds., 45 pgs. (In Press).

Wheeler, M.B. A.S. Lima, T. VanEtten, ad M.R.B. Mello. 2007. Transgenic Animals for Biomedicine and Agriculture. In Bovine Theriogenology, de la Sota, R.L. and Bo, G.A., Eds. 36 pgs. (In Press).

Wrench, N. Effect of season on expression of SP22 and mRNAs in equine spermatozoa. MS Thesis, North Carolina State University. 2007.

3. ABSTRACTS

Banks, A., M. Looper, S. Reiter, L. Starkey, R. Flores, D. Hallford, and C. Rosenkrans, Jr. 2007. Identification of single nucleotide polymorphisms within the promoter region of the bovine heat shock protein 70 gene and associations with pregnancy. J. Anim. Sci. 85 (Suppl. 2):12.

Barcelo-Fimbres, M., and G.E. Seidel, Jr. 2007. Validation of quantitative fluorescence for determining lipid content of bovine blastocysts stained with Nile Red dye. Biol. Reprod. (Special Issue) pp. 87-88.

Campos-Chillón, L.F., M. Barcelo-Fimbres, E.M. Carnevale, and G.E. Seidel, Jr. 2007. Use of bovine oocytes to evaluate in vitro fertilizing capacity of equine sperm. Theriogenology 68:515-516.

Chang, C.C., X.C. Tian, X. Yang, R.J. Straub, D.L. Keenan, and Z.P. Nagy. The effect of cryoprotectants on preserving oocyte spindle following sub-physiological temperature treatment. Fertil Steril 2007; 88(1S):S89. Oral (Washington DC, American Society for Reproductive Medicine in 2007 annual meeting).

Chang, C.C., X.C. Tian, X. Yang, D.B. Shapiro, S.M. Slayden, and Z.P.Nagy. Oocyte spindle preservation during vitrification of mouse oocytes. Fertil Steril 2007; 88(1S):S94. Oral (Washington DC, American Society for Reproductive Medicine in 2007 annual meeting).

Chang, C.C., L.Y. Sung, X.C. Tian, X. Yang, H.I. Kort, and Z.P. Nagy. Parallel comparison of parthenogenetic development following oocyte cryopreservation: vitrification vs. slow freezing. Fertil Steril 2007;88(1S):S350. Poster (Washington DC, American Society for Reproductive Medicine in 2007 annual meeting).

Chaubal, S.A., T.L. Nedambale, J. Xu, C. Shaffer, T. Kilmer, A. Shefle, X. Yang, X.C. Tian, and F. Du. 2008. Effect of Heparin Concentration on Bovine Preimplantational Development in vitro using Sex-sorted Sperm (to be presented in the 2008 IETS annual meeting, Denver, CO, Jan 5-9, 2008).

Curry E., S. L. Pratt, D. L. Lapin, and J. R. Gibbons. 2007. High-throughput genetic characterization of bovine in vivo produced embryos. Biol Reprod 2008 Special Issue:440.

Dewey, S.T., C.W. Wall, M.L. Looper, C.F. Rosenkrans, Jr., A.H. Brown, Jr., and Z.B. Johnson. 2007. The relationship of dam efficiency and calf performance to weaning in a commercial cowherd. J. Anim. Sci. 85 (Suppl. 2):5.

Everts, R.E., A. Sommers, C.A. Green, R.Oliveira, S. Rodriguez-Zas, L.Y. Sung, F. Du, A.C.O. Evans, M. Boland, T. Fair, P. Lonergan, J.P. Renard, X. Yang, H.A. Lewin, and X.C. Tian. Major differences in Gene expression profiles revealed in Day-25 placental tissues collected from Cows carrying cloned fetuses (to be presented in the Plant & Animal Genome XV Conference, January 13-17, 2007, San Diego, USA).

Farmer, W.T., P.W. Farin, S.R. Bischoff, J.E. Alexander, J.A. Peidrahita, and C.E. Farin. Detection of antisense to Igf2r (AIR) RNA in cattle. Reprod Fertil Dev 20:Abstract #2 (In Press).

Farin, P.W., D.E. Malarkey, J.E. Alexander, and C.E. Farin. Liver and kidney function in bovine fetuses from embryos produced in vivo or in vitro. Reprod Fertil Dev 20: Abstract #201 (In Press).

Feltrin, C., A.S. Lima, E/ Monaco, S.M. Wilson,, D. Kim, T. VanEtten, S.J. Lane, J.L. Rodrigues,, and M.B. Wheeler. (2007). Efficiency of Electrical And Chemical Activation in Swine Embryonic Structures Produced by Hand-Made Cloning. Acta Scientiae Veterinariae 35 (Supl. 3):1277.

Feugang, J.M., A. Kaya, E. Topper, C. Ostermeier, and E. Memili. 2007. Transcripts of bull spermatozoa ). Abstract presented at the annual meeting of the Society for the Study of Reproduction (SSR) Biol Reprod. Special issue.

Flores, R., M.L. Looper, S.T. Reiter, and C.F. Rosenkrans, Jr. 2007. Effects of body condition, somatotropin, and prolactin promoter gene polymorphisms on concentrations of prolactin, follicle size, and calving rates of postpartum Brahman-influenced cows. J. Anim. Sci. 85 (Suppl. 2):12.

Floyd, C. F., S. Gray, L. M. Riggs, and J. R. Gibbons. 2007. A comparison of two sustained FSH release implants. Biol Reprod 2008 Special Issue:223.

Gong, G.C., M. Roach, S. Jiang, X. Yang, and X.C. Tian. 2007. Effects of leukemia inhibitory factor (LIF) and feeder on derivation of embryonic stem cells from in vitro fertilized bovine blastocysts (presented in StemConn07, Hartford, CT, Mar 2007).

Katz-Jaffe, M.G., B.R. McCallie, K. Preis, G. Seidel, and D.K. Gardner. 2007. In vitro maturation (VM) alters the expression of specific putatively imprinted genes in bovine MII oocytes. Reprod. Fertil. Develop. 19:251.

Kim, D., A. Lima, E. Monaco, I. Meyvantsson, D.J. Beebe, M.B. Wheeler. (2007) Mesenchymal Stem Cell Culture and Differentiation in High-Throughput Microdevices Using a Fluid Handling Robot. Adult Mesenchymal Stem Cells in Regenerative Medicine (MSC2007), Cleveland, OH, USA, Abstr. 210.

Krause, M, S. Gray and J. R. Gibbons. 2007. Endocrine dynamics surrounding dominant follicle selection in cattle. Biol Reprod 2008 Special Issue:402.

Krisher, R.L. 2007. Elucidating the mechanisms involved in oocyte quality by examining gene expression. J. Anim. Sci. 85 (Suppl. 2):116.

Krisher, R., A. Auer, K. Clark, K. Emsweller, S. Rogers, K. Thomas, F. Chatiza, and P. Bartels. 2007. In vitro production of springbok (Antidorcas marsupialis) embryos. Reprod Ferti. De. 19:239-240.

Krisher, R., J. Lowe, A. Bailey, and M. Sturek. 2008. Endocrine parameters and ovarian dynamics in Ossabaw swine suggest a model for polycystic ovary syndrome. Exp. Biol. (Accepted).

Kubota, C., T. Kojima, T. Nagai, X. Tian, and X. Yang. 2007. Bovine embryo development after IVM/IVF/IVC of oocytes stored for 22 h in various media (presented in the annual meeting of IETS in Kyoto Japan, Jan 2007).

Lamb, M., R. Okimoto, M. Brown, H. Brown, Jr., Z. Johnson, and C. Rosenkrans, Jr. 2007. Relationship between single nucleotide polymorphisms in the bovine heat shock protein 70 gene and milk characteristics of beef cows. J. Anim. Sci. 85 (Suppl. 2):4.

Looper, M.L., T.P. Neidecker, C.W. Wall, S.T. Reiter, R. Flores, A.H. Brown, Jr., Z.B. Johnson, and C.F. Rosenkrans, Jr. 2007. Relationship of lactate dehydrogenase activity to body measurements of Angus x Charolais cows and calves. J. Anim. Sci. 85 (Suppl. 2):31.

Lowe, J., R.L. Krisher, and M. Sturek. 2007. Characterizing the Ossabaw mini-pig as an animal model for polycystic ovary syndrome. Biol. Reprod. 2008 Special Issue:211.

Lowe, J.L. and R.L. Krisher. 2007. In vitro maturation of porcine oocytes in two defined media with and without gonadotropins. J. Anim. Sci. 85 (Suppl. 2):69.

Lucius, H. and J. R. Gibbons. 2007. The use of bovine follicular fluid and fetal calf serum as supplements for bovine oocyte in vitro maturation. Sixth Annual Summer Program for Research Interns, Clemson University, Clemson SC.

Marshall, K.M., W.L. Hurley, R.D. Shanks, and M.B. Wheeler. (2007). Effects of suckling intensity on milk yield and piglet growth from lactation-enhanced gilts. Reproduction, Fertility and Development 19:318-319. Abstr. 406.

Meece, A., S. He, and C.L. Keefer. 2007. Expression of LEF1 transcription factor and its novel isoforms in preimplantation embryos. Reprod. Fert. Dev. 19(1):252.

Paczkowski, M.J., C. Bidwell, B. Craig, A. Lipka, J. Waddell, and R. Krisher. 2007. Effect of puberty and follicle size on gene expression in porcine oocytes. Biol. Reprod. 2008 Special Issue:229.

Paczkowski, M.J., C. Bidwell, J. Waddell, D. Spurlock, and R.L. Krisher. 2008. In vitro maturation alters gene expression in mouse oocytes. Reprod Fertil Dev (In Press).

Park, J.H., S.L. Smith, L. Lai, M. Samuel, D. Wax, R.S. Prather, X. Yang, and X.C. Tian. 2007. Expression profiles of brain, kidney and lung of cloned pigs (to be presented in annual meeting of SSR, San Antonio, TX Jul 2007).

Person, C.N., T. D. Lester, M. D. Person, and R. W. Rorie. 2007. Computer-assisted analysis of sperm parameters after selection of motile sperm by either percoll gradient, filtration or swim-up procedures. Southern Section, ASAS (accepted)

Person, C.N., T. D. Lester, M. D. Person, and R. W. Rorie. 2007. Computer-assisted analysis of sperm parameters after selection of motile sperm by either percoll gradient, ltration or swim-up procedures. J Anim Sci 85 (Suppl 1):538.

Pope, C., S. Reiter, M. Brown, Y. Farnell, D. Baker, H. Brown, Jr., Z. Johnson, and C. Rosenkrans, Jr. 2007. Relationship between polymorphisms in lactate dehydrogenase B gene and milk characteristics in beef cows. J. Anim. Sci. 85 (Suppl. 2):44.

Preis, K.A., G.E. Seidel, Jr., and D.K. Gardner. 2007. Effect of in vitro maturation medium on subsequent quantity and quality of developing embryos. Proc. American Society of Reproduction Medicine (In Press).

Pryor, J.H., C.R. Looney, D. Walker, G.E. Seidel, Jr., J.F. Hasler, D.C. Kraemer, and S. Romo. 2007. Comparison between conventional direct transfer freezing and vitrification for the cryopreservation of in vivo embryos from Brahman cattle. Reprod Fertil Develop 19:224-225.

Purcell, S., J. Cantlon, L. Henkes, G.E. Seidel, Jr. and R. Anthony. 2007. Expression of periattachment factor mRNA during ovine conceptus development. Biol Reprod (Special Issue), pp. 176-177.

Rasheed E. and J. R. Gibbons. 2007. Endocrine dynamics of the per-pregnancy establishment period in dairy cattle. Sixth Annual Summer Program for Research Interns, Clemson University, Clemson SC.

Reiter, S., M. Larson, R. Okimoto, M. Brown, H. Brown, Jr., Z. Johnson, and C. Rosenkrans, Jr. 2007. Genomic comparisons of bovine CYP3A28 of Angus, Brahman, and reciprocal crosses and milk characteristics. J. Anim. Sci. 85 (Suppl. 2):3.

Seidel, G.E., Jr., M.L. Turk, P.W. Gordy, and R.A. Bowen. 2007. Recombinant bovine trypsin made in maize inactivates bovine herpes virus-1 adsorbed to the bovine zona pellucida. Reprod Fertil Dev. 19:236.

Smith, S.L., X. Vignon, Y. Heyman, H.A. Lewin, X.C. Tian, J.P. Renard, and X. Yang. 2007. Different global gene expression profiles of Single, Cloned bovine embryos with different developmental competencies, The Good, the Bad and the Ugly. (to be presented in annual meeting of SSR, San Antonio, TX Jul 2007).

Starkey, L., M. Looper, A. Banks, S. Reiter, and C. Rosenkrans, Jr. 2007. Identification of polymorphisms in the promoter region of the bovine heat shock protein gene and associations with bull calf weaning weight. J. Anim. Sci. 85 (Suppl. 2):44.

Suh, T.K., and G.E. Seidel, Jr. 2007. Effect of supplementary inositol 1,4,5-tri-phosphate injection on fertilization and embryo development after bovine ICSI. Reprod Fertil Dev 19:305.

Sung, L.Y., S. Gao, H. Shen, H. Yu, Y. Song, S.L. Smith, K. Inoue, D.P. Tuck, S.M. Weissman, X.C. Tian, T. Cheng, and X. Yang. Are differentiated cells more efficient than adult stem cells for somatic cell cloning? (presented in StemConn07, Hartford, CT, Mar 2007).

Tejomurtula, J. and J. Yao. 2007. Identification of a novel importin alpha predominantly expressed in bovine oocytes and early embryos. Biol. Reprod. 2007 Special Issue:218.

Tian, X.C., S.L. Smith, R.E. Everts, P. Lonergan, F. Du, L.Y. Sung, S. Zhang, L. Yang, C. Curchoe, F. Xue A. Sommers, C.A. Green, R. Oliveira, S. Rodriguez-Zas, A.C.O. Evans, M. Boland, T. Fair, H.A. Lewin, X. Yang, X. Tian. Analyses of somatic cell nuclear reprogramming by the candidate and global gene expression approaches (to be presented in the 2nd International Meeting on Mammalian Embryogenomics, Paris, France, Oct 17-20, 2007).

Tian, X.C. .Safety Concerns for Foods from Cloned Animals (to be presented in the BioEco2007, Tianjin, PR China, June 2-28, 2007).

Tian, X.C. The use of DNA microarray technology to study reproduction in domestic animals (to be presented in 14th International Conference on Biotechnology in Animal Reproduction, ICBAR, Cairo, Eygpt, Aug 6-7. 2007).

Walker, D.J., C.J. Wilusz, and G.E. Seidel, Jr. 2007. Timing of deadenylation of GDF-9 and cyclin 3 UTR constructs in bovine oocytes. Reprod Fertil Dev 19:121.

Wang, H., J.M, Feugang, N. Rodriguez-Osorio, S. Jung, K.F. Garrison, C. Wolgemuth, L. Greer, and E. Memili. 2007. Developmental biology of porcine preimplantation embryos in vitro. Abstract presented at the annual meeting of the Society for the Study of Reproduction (SSR) Biol Reprod. Special issue.

Wheeler, M.B., E. Monaco, A.S. Lima, S. Wilson, D. Kim, and M. Bionaz. (2007). Identification of Reference Genes for qPCR and PPARG Gene Expression During Adipogenic Differentiation of Porcine Adult Mesenchymal Stem Cells, Convergence of Genomics and the Land-Grant Mission: Emerging Trends in the Application of Genomics in Agricultural Research , West Lafayette, Indiana, September 10-12, 2007, Abstr. 136.

Yang, X., S.L. Smith, X.C. Tian, H.A. Lewin, J.P Renard, and T Wakayama. 2007. Nuclear reprogramming of cloned embryos - implications for therapeutic cloning (presented in StemConn 07, Hartford, CT, Mar 2007).

Yuan, Y. and R. Krisher. 2007. Replacing glutamine with alanyl-glutamine in chemically defined maturation medium can increase porcine oocyte nuclear maturation in vitro. Biol Reprod 2008 Special Issue:224.

Yuan, Y. and R.L. Krisher. 2008. The presence of ammonium in chemically defined maturation medium inhibits porcine oocyte nuclear maturation and subsequent embryonic development in vitro. Reprod Fertil Dev (In Press).

4. MISCELLANEOUS PUBLICATIONS (SEMI-TECHNICAL/LAY PUBLICATIONS)

Amann, R.P. and G.E. Seidel, Jr. 2007. The sex ratio riddle. Beef 43:57-58.

Bogucki, J . Extraction and quantification of mitochondria from mouse livers. Undergraduate research report. Aug, 2007.

Chen, J. (University Scholar). Derivation and characterization of mouse Oct4-GFP embryonic stem cells, Honors thesis. May, 2007.

Coffey, K.P., W.K. Coblentz, J.D. Caldwell, C.P. West, R.K. Ogden, T. Hess, D.S. Hubbell, III, M.S. Akins, and C. F. Rosenkrans, Jr. 2007. Cow and calf performance while grazing tall fescue pastures with either the wild-type toxic endophyte or a non-toxic novel endophyte. Arkansas Agricultural Experiment Station (AAES) Research Series 553:67-69.

Ettema, J.F., D.L. Hoag, and G.E. Seidel, Jr. 2007. Economic opportunities for use of sexed semen on commercial dairy farms. Colorado Dairy News, 4 pp.

Farin, P.W. 2007. Current techniques in bovine embryo transfer. Proc SAVMA Symposium 2007, Raleigh, NC, March 15-17, 2007.

Farin, P.W.. 2007. Designing estrus synchronization programs for cattle. Proc SAVMA Symposium 2007, Raleigh, NC, March 15-17, 2007.

Farin, P.W. and C.E. Farin. 2007. Reproductive technologies&Are they a fit for your operation? The Carolina Cattlemen's Connection, Jan. Issue.

Keefer, C.L., Pommer, J, and J.M. Robl. 2007. The Role of Transgenic Livestock in the Treatment of Human Disease: Animal Agricultures Future through Biotechnology, Part 6. Council for Agricultural Science and Technology (CAST) Issue Paper 35.

Kreider, D.L., K.P. Coffey, J.D. Caldwell, W.A. Whitworth, T.G. Montgomery, R. Rorie, R.W. McNew, W. Coblentz, and R.K. Ogden. 2007. Reproductive performance, blood urea nitrogen, and blood glucose concentration in beef heifers grazing annual ryegrass in the spring and supplemented at different intervals prior to timed AI. AAES Research Series 55:41-44.

Looper, M. L., T. P. Neidecker, C. W. Wall, S. T. Reiter, R. Flores, A. H. Brown, Jr., Z. B. Johnson, and C. F. Rosenkrans, Jr. 2007. Relationship of lactate dehydrogenase activity with body measurements of Angus x Charolais cows and calves. Arkansas Agricultural Experiment Station (AAES) Research Series 553:13-15.

Park, J.H., S. Smith, C. Curchoe, L. Lai, M. Samuel, D. Wax, R. Prather, X. Yang, and X.C. Tian. 2007. Nuclear Reprogramming Is Not Associated With Maternal Lineage Of Recipient Oocytes. College of Agriculture and Natural Resources Graduate Forum. University of Connecticut.

Person, C.N., T.D. Lester, M.D. Person, and R.W. Rorie. 2007. Effects of penicillamine, hypotaurine and epinephrine (PHE) on post-thaw bovine sperm parameters, as measured by computer-assisted sperm analysis. Arkansas Agricultural Experiment Station (AAES) Research Series 55:49-52.

Person, C.N., T.D. Lester, M.D. Person, and R.W. Rorie. 2007. Computer-assisted analysis of sperm parameters after selection of motile sperm by either percoll gradient, filtration or swim-up procedures. Arkansas Agricultural Experiment Station (AAES) Research Series 55:53-55.

Powell, J.G., A.H. Brown, T.A. Yazwinski, R.A. Rorie, Z.B. Johnson, and J.L. Reynolds. 2007. Influence of reproductive tract score on pregnancy in angus heifers. Arkansas Agricultural Experiment Station (AAES) Research Series 55:39-40.

Rorie, R.W. and T.D. Lester. 2007. Effect of bovine somatotropin treatment on AI pregnancy rate in dairy heifers. Arkansas Agricultural Experiment Station (AAES) Research Series 55: 47-48.

Seidel, G.E., Jr. 2007. Superovulation in mammals. In: The Encyclopedia of Biotechnology in Agriculture and Food, D.R. Holdman, A. Bridges, D. Hoover and M.B. Wheeler, eds. Marcel Dekker, NY.

Seidel, G.E., Jr. 2007. Use of ultrasound for pregnancy diagnosis in cattle. National Cattleman, Special Producer Edition, p. 15.

VanEtten, T.L. and M.B. Wheeler. 2007. Evaluation of a novel electronic estrus detection device. Illinois Dairy Report: Getting it Right, pgs 74-76.

Wang, H., T. Spires, and E. Memili. 2007. Test Tube Pigs are Now a Reality. National Hog Farmer December 2007 issue.

Wheeler, M.B. 2007. The use of gender-selected semen in dairy production schemes. Illinois Dairy Report: Getting it Right, pgs 72-73.

04/05/2009

1. Refereed articles/book chapters

Anupriwan, A. et al. 2008. Presence of arylsulfatase a and sulfogalactosylglycerolipid in mouse ovaries: Localization to the corpus luteum. Endocrinology 149: 3942-3951.

At-Taras, E. E., I. C. Kim, T. Berger, A. Conley, and J. F. Roser. 2008. Reducing endogenous estrogen during development alters hormone production by porcine leydig cells and seminiferous tubules. Domest Anim Endocrinol 34: 100-108.

Bass, B.E., R.A. Cederberg, G.A. Mills, and B.R. White. 2008. Responsiveness of GnRH receptor and gonadotropin subunit genes to GnRH in lines of swine divergent for ovulation rate. Biol. Reprod. (Submitted).

Berger, T. et al. 2008. Reducing endogenous estrogens during the neonatal and juvenile periods affects reproductive tract development and sperm production in postpuberal boars. Anim Reprod Sci 109: 218-235.

Berger, T., and B. Roberts. 2008. Reduced immunolabelling of a porcine oocyte membrane protein reflects reduced fertilizability of porcine oocytes following elevated ambient temperature. Reprod Domest Anim.

Bischoff SR, Tsai, S, Hardison NE, York AM, Freking BA, Nonneman D, Rohrer G, Piedrahita JA. 2008. Identification of SNPs and INDELS in swine using short oligonucleotide microarrays. BMC Genomics 9:252- 263. PMC2442091.

Bleck, G.T., Wheeler, M.B., Hansen, L.B., Chester-Jones, H., Miller, D.J. 2008. Lactose Synthase Components in Milk: Concentrations of Alpha-Lactalbumin and ²1,4-Galactosyltransferase in Milk of Cows from Several Breeds at Various Stages of Lactation. Reprod. Dom. Anim. doi: 10.1111/j.1439-0531.2007.01047.xISSN 0936-6768.

Campos-Chillon, L.F., T.K. Suh, M. Barcelo-Fimbres, G.E. Seidel, Jr. and E.M. Carnevale. 2008. Vitrification of early-stage bovine and equine embryos. Theriogenology 70:In Press.

Cederberg, R.A., E.A. McDonald, C. Lee, R.A. Friedrich, A.R. Cropp, and B.R. White. 2008. Activity of the porcine GnRH receptor gene in aT3-1 cells is partially conferred by a distal swine upstream promoter enhancing region (SUPER) and two proximal steroidogenic factor-1 binding sites. Endocrinology (Submitted).

Chang CC, Sung LY, Kort HI, X Yang, XC Tian, ZP Nagy. The oocyte spindle is preserved by 1, 2-propanediol during slow-freezing. Fert Sertil. 2009 (in press).

Chapman, P.L. and G.E. Seidel, Jr. 2008. Experimental design, power, and sample size for animal reproduction experiments. Reprod. Fertil. Dev. 20:33-44.

Cherrington, B.D., T.A. Farmerie, B.E. Bass, R.A. Cederberg, C.M. Clay, and B.R. White. 2008. Emergence of a new regulatory element in the proximal promoter of the gonadotropin releasing hormone receptor in Chinese Meishan swine. Biol. Reprod. (Accepted).

Curry, E., S. Pratt, D. Kelley, D. Lapin, J. Gibbons. 2008. Use of combined duplex PCR/dot blot assay for more sensitive genetic characterization. Biochem. Insights 2008:I:43-47.

Dinnyes A, XC Tian, X Yang. 2008. Epigenetic regulation of fetal development in nuclear transfer animal models (presented in the 16th International Congress on Animal Reproduction, and a special issue of the Reproduction in Domestic Animals) Reprod Dom Anim 43: Suppl 2: 302-309.

Du F, Xu J, Zhang JF, Gao SR, Carter MG, He CL, Sung, LY, Chaubal S, Fissore R, Tian XC, Yang, X, Chen YE. 2009. Beneficial effects of young oocytes in rabbit somatic cell nuclear transfer. Cloning and Stem Cells (in press).

Estrada JL, Collins B, York A, Bischoff SR, Sommer J, Tsai S, Petters RM, Piedrahita JA. 2008. Successful cloning of the Yucatan minipig using commercial/occidental breeds as oocyte donors and embryo recipients. Cloning and Stem Cells 10:287-296.

Everts RE, Chavatte-Palmer P, Razzak A, Hue I, Green CA, Oliveira R, Vignon X, Rodriguez-Zas SL, Tian XC, Yang X, Renard JP, Lewin HA. 2008. Aberrant gene expression patterns in placentomes are associated with phenotypically normal and abnormal cattle cloned by somatic cell nuclear transfer. Physiol Genomics 33:65-77.

Feugang JM, Camargo-Rodriguez OD and Memili E. Culture Systems for Bovine Embryos. (2008) In press. Livestock Science.

Feugang JM, Kaya A, Page GP, Chen L, Mehta T, Hirani K, Nazareth L, Gibbs RA and Memili E. Two stage genome wide association study identifies Integrin beta five as having a potential role in bull fertility. (2008) In press. BMC Genomics.

Flores, R., M.L. Looper, R.W. Rorie, D.M. Hallford and C.F. Rosenkrans, Jr. 2008. Endocrine factors and ovarian follicles are influenced by body condition and somatotropin in postpartum beef cows. . J. Anim. Sci. 86:1335-1344.

Garner, D.L. and G.E. Seidel, Jr. 2008. History of commercializing sexed semen for cattle. Theriogenology 69:886-895.

He, S., Pant, D., Schiffmacher, A., Meece, A.,Keefer, C.L. (2008). Lef1 mediated Wnt signaling promotes the initiation of trophoblast lineage differentiation in mouse embryonic stem cells Stem Cells 26:842-849.

Horvath, G. and G.E. Seidel, Jr. 2008. Use of fetuin before and during vitrification of bovine oocytes. Reprod. Dom. Anim. 43:333-338.

Hyland, A., J.C. Whittier, G.E. Seidel, Jr., R.M. Enns and R.K. Peel. 2008. Variations in interval between GnRH and prostaglandin F2alpha injections and CIDR insertion for 5 or 7 days on pregnancy rates and follicular response. Proc. West. Sect. Am. Soc. Anim. 59:1-4.

Jiang L, Lai L, Samuel M, Prather RS, Yang X, Tian XC. 2008. Expression of X-linked Genes in Deceased Neonates and Surviving Cloned Female Piglets. Mol Reprod Dev 75:265-73.

Keefer, C.L. (2008) Lessons learned from nuclear transfer (cloning). Theriogenology 69:48-54.

Kim C, Amano T, Park J, Carter MG, Tian X, Yang X. Improvement of Embryonic Stem Cell Line Derivation Efficiency with Novel Medium, Glucose Concentration, and Epigenetic Modifications 2009. Cloning and Stem Cells (in press).

Lee E, Estrada JL, Piedrahita JA. 2008. A comparative study on the efficiency of two enucleation methods in pig somatic cell nuclear transfer: effects of the squeezing and the aspiration methods. Animal Biotechnology 19:71-79.

Li C, Bin YF, Tian XC, Curchoe C, Yang L, Feng DY, Jiang QY, ONeill M, Zhang SQ. 2008. Genetic imprinting of H19 and IGF2 in domestic pigs (Sus scrofa). Animal Biotechnology;19:22-7.

Li GP, Yang S, Liu Y, Sessions BR, White KL, Bunch TD. 2008. Nicotine combined with okadaic acid or taxol adversely affects bovine oocyte maturation and subsequent embryo development. Fertil Steril. Sep 13. [Epub ahead of print]

Liu Y, Li GP, Rickords LF, White KL, Sessions BR, Aston KI, Bunch TD. 2008. Effect of nicotine on in vitro maturation of bovine oocytes. Anim Reprod Sci. 103:13-24.

Liu Y, Li GP, Sessions BR, Rickords LF, White KL, Bunch TD. 2008. Nicotine induces multinuclear formation and causes aberrant embryonic development in bovine. Mol Reprod Dev. 75:801-9.

Liu Y, Li GP, White KL, Rickords LF, Sessions BR, Aston KI, Bunch TD. 2007. Nicotine alters bovine oocyte meiosis and affects subsequent embryonic development. Mol Reprod Dev. 74:1473-82.

Looper, M.L., R.W. Rorie, C.N. Person, T.D. Lester, D.M. Hallford, G.E. Aiken and C.F. Rosenkrans, Jr. 2008. Influence of toxic endophyte-infected fescue on sperm characteristics and endocrine factors of yearling Brahman-influenced bulls. J. Anim. Sci. (in press).

Lopez-Garcia, M. d. C.; Monson, R. L.; Haubert, K.; Wheeler M. B.; Beebe, D. J. (2008). Sperm Motion in a Microfluidic Fertilization Device. Biomedical Microdevices 10.1007/s10544-008-9182-7

Lopez-Garcia, M. d. C.; Monson, R. L.; Haubert, K.; Wheeler M. B.; Beebe, D. J. (2008). Sperm Motion in a Microfluidic Fertilization Device. Biomed Microdevices 10:709718.

Mahmoud, K.G.M., T.H. Scholkamy, Y.F. Ahmed, G.E. Seidel, Jr. and M.F. Nawito. 2008. Effect of different combinations of cryoprotectants on in vitro maturation of immature buffalo (Bubalus bubalis) oocytes vitrified by straw and open-pulled straw methods. Reprod. Dom. Anim. In Press.

Mazur, P., S.P. Leibo and G.E. Seidel, Jr. 2008. Cryopreservation of the germplasm of animals used in biological and medical research: Importance, impact, status, and future directions. Biol. Reprod. 72:2-12.

McDonald, E.A., J.E. Smith, R.A. Cederberg, and B.R. White. 2008. Divergent activity of the porcine GnRH receptor gene promoter in aT3-1 cells is partially conferred by nuclear factor (NF)-kB, specificity protein (Sp)1-like, and GATA4 elements. Endocrinology (Submitted).

Miles, J.R., Blomberg, L.A., Krisher, R.L., Everts, R.E., Sonstegard, T.S., Van Tassell, C.P., Zuelke, K.A. (2008) Small amplified RNA-serial analysis of gene expression (SAR-SAGE) from porcine blastocysts produced in vivo or in vitro. Molecular Reproduction and Development. 75:976-988. DOI: 10.1002/mrd.20844.

Montagner, M.M, A.R. Cropp, J.J. Swanson, R.A. Cederberg, P.B.D. Goncalves, and B.R. White. 2008. Role of gonadotropin-releasing hormone on mouse preimplantation embryonic development. Mol. Reprod. Dev. (Submitted).

Montagner, M.M, P.B.D. Goncalves, G.A. Mills, R.K. Christenson, and B.R. White. 2007. Divergent survival of Meishan and white crossbred porcine embryos following vitrification using a microdroplet method. Reprod. Fert. Dev. (Submitted).

Niemann H, Tian XC, King WA, Lee R. 2008. Epigenetic reprogramming in bovine embryonic and foetal development upon somatic cell nuclear transfer cloning. Reproduction (in press).

Pate BJ, White KL, Chen D, Aston KI, Sessions BR, Bunch TD, Weimer BC. 2008. A novel approach to identify bovine sperm membrane proteins that interact with receptors on the vitelline membrane of bovine oocytes. Mol Reprod Dev. 75:641-9.

Peddinti D, Nanduri B, Kaya A, Feugang JM, Burgess SC and Memili E. Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. (2008) BMC Systems Biology 2:19. Doi:10.1186/1752-0509-2-19.

Pond, W.G., Mersmann, H.J., Su, D., McGlone, J.J., Wheeler, M.B., OBrian Smith, E. (2008). Neonatal Dietary Cholesterol and Alleles of Cholesterol 7-a Hydroxylase, Affect Piglet Cerebrum Weight, Cholesterol Concentration, and Behavior. J. Nutr. 138:282-286.

Rodriguez-Osorio N, Wang Z, Kasinathan P, Page GP, Robl JM and Memili E. Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos. (2008). In press. BMC Genomics.

Rodriguez-Osorio N, Wang Z, Kasinathan P, Page GP, Robl JM, and Memili E. Transcriptional Reprogramming of Gene Expression in Bovine Somatic Cell Chromatin Transfer Embryos. (2008) In press. BMC Genomics.

Schenk, J.L., D.G. Cran and G.E. Seidel, Jr. 2008. Pregnancy rates in heifers and cows with cryopreserved sexed sperm: effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting. Theriogenology. In Press.

Seidel, G.E., Jr. 2008. ASAS Centennial Paper: Future research in physiology and endocrinology. J. Anim. Sci. In Press.

Seidel, G.E., Jr. and J.L. Schenk. 2008. Pregnancy rates in cattle with cryopreserved sexed sperm: Effects of sperm numbers per inseminate and site of sperm deposition. Anim. Reprod. Sci. 105:129-138.

Silva, C.,* J.R. Wood*, L. Salvador, I. Kostetskii, C.J. Williams, and J.F. Strauss III 2009. Expression profile of male germ cell-associated genes in mouse embryonic stem cell cultures treated with all-trans retinoic acid and testosterone. Mol. Reprod. Dev. 76:11-21. *authors contributed equally to the manuscript

Smith SL, Everts RE, Sung LY, Du F, Page RL, Henderson B, Rodriguez-Zas SL, Nedambale TL, Renard JP, Lewin HA, Yang X, Tian XC. 2008. Gene expression profiling of single bovine embryos uncovers significant effects of in vitro maturation, fertilization and culture. Mol Reprod Dev. 2008 Apr 30 [Epub ahead of print]

Stevenson, J.S., Tenhouse, D.E., Krisher, R.L., Lamb, G.C., Larson, J.E., Dahlen, C.R., Pursley, J.R., Bello, N.M., Fricke, P.M., Wiltbank, M.C., Brusveen, D.J., Burkhart, M., Youngquist, R.S., Garverick, H.A. (2008) Detection of Anovulation by Heatmount Detectors and Transrectal Ultrasonography before Treatment with Progesterone in a Timed Insemination Protocol. Journal of Dairy Science. 91:2901-2915.

Uzun A, Rodriguez-Osorio N, Wang H, Kaya A, Parrish JJ, Ilyin V and Memili E. Comparative Functional Genomics of Chromatin Remodeling Proteins HMGN3a and SMARCAL1 During Early Mammalian Embryogenesis. (2008) In press. BMC Genomics.

Wang H, Feugang JM, Rodriguez-Osorio N, Jung S, Garrison K, Wolgemuth C, Greer L, Memili E. Biology of Preimplantation Porcine Embryos in vitro. (2008) In press. Livestock Science.

West, F. D., D. W. Machacek, N. L. Boyd, K. Pandiyan, K. R. Robbins, S. L. Stice. 2008. Enrichment and Differentiation of Human Germ-Like Cells Mediated by Feeder Cells and Basic Fibroblast Growth Factor Signaling. Stem Cells. 11: 2768-76.

West, F. D., M. I. Roche-Rios, S. Abraham, R. R. Rao, M. S. Natrajan, and S. L. Stice. Bone Morphogenetic Protein and KIT Ligand Signaling Enhances Human Embryonic Stem Cell to Germ-Like Cell Differentiation. Submitted (11/21/08).

Wheeler, M.B. Lima, A.S., VanEtten, T., Mello, M.R.B., (2008). Transgenic Animals for Biomedicine and Agriculture. In, Bovine Theriogenology, de la Sota, R.L. and Bo, G.A., Eds. 36 pgs. (in press).

Wheeler, M.B., Mello, M.R.B., Lima, A.S., VanEtten, T. 2008. Modification of the Domestic Animal Genome. In, Bovine Theriogenology, de la Sota, R.L. and Bo, G.A., Eds., 45 pgs. (in press).

Yang X, Guo XM, Wang CY, and Tian XC. 2009. Protocols for Large-Scale Derivation of Cardiomyocytes from Embryonic Stem Cells Chapter 22. In: Essentials of Stem cells. Elsevier, Lanza R (ed). (in press).

Zaunbrecher G, Mir B, Dunne PW, Breen M, Piedrahita JA. 2008. Enhancement of extra chromosomal recombination in somatic cells by affecting the ratio of homologous recombination (HR) to non-homologous end joining (NHEJ). Animal Biotechnology 19:6-21.

Zhang, Z., R. Jaimez, X. Shen, D. Gude, H. Tang, J.R. Wood, E. Goldberg, and J.F. Strauss III. 2008. Autoregulation of SPAG16 expression: A single gene encoding an axoneme structural protein and a transcription factor that activates the axoneme protein promoter. Nucleic Acids Res. (Submitted).

2. Books, non-refereed book chapters, proceedings, instructional media, theses/dissertations

Barfield, J.P., P.M. McCue, G.E. Seidel, Jr. and E.L. Squires. 2008. The effect of dehydrating large equine embryos with galactose prior to cryopreservation. Proc. 77th Int. Symp. Equine Embryo Transfer, pp. 65-66.

Bormann, C. L., M. B. Wheeler, D. J. Beebe, S. Takayama, and G. D. Smith. 2008. Microfluidics for Assisted Reproductive Technologies. In, Micro- and Nanoengineering of the Cell Microenvironment: Technologies and Applications", eds. A. Khademhosseini, J. Borenstein, S. Takayama, M. Toner. Chapter 11, pages 209-225, Artech House Publishing Inc.

Delgado Callisaya, P.A. 2008. Factors influencing survival of bovine spermatozoa during storage and related to the ratio of X- to Y-chromosome bearing spermatozoa. M.S. Thesis, University of Arkansas, Fayetteville.

Denniston, R. S., S. Michelet, K.R. Bondioli and R. A. Godke. Principles of Embryo Cryopreservation. 2008. In: Cryopreservation in Aquatic Species. Tiersch, T. R. and P. M. Mazik, Editors. World Aquaculture Society, Baton Rouge, Louisiana. (In press).

Farmer, WTQ. Detection of antisense to IGF2R (AIR) RNA in cattle. MS Thesis, North Carolina State University. 2008.

Godke, R.A., G. Gentry Jr. and K. R. Bondioli. 2008. Advances in cattle reproductive technologies. Proc. WBC (May 18-23) Ft Worth, Texas. ABBA. CD Series 16 pages.

Hongfeng Wang. Porcine embryogenesis and effects of mycotoxins on early pig development. Ph.D. Thesis.

Howland, EM. 2008. Evaluation of a polymorphism in the prolactin gene as a potential genetic marker for mastitis susceptibility. Undergraduate Honors Thesis, University of Arkansas, Fayetteville.

Hudson, M.R. In vitro capacitation of stallion spermatozoa as assayed by fertilization of bovine oocytes. M.S. Thesis, Colorado State University, Fort Collins, CO.

Kuchenbrod, LM. Effect of serum and hormone supplementation on competence of bovine oocytes selected by brilliant cresyl blue staining. MS Thesis, North Carolina State University. 2008.

Looney, C.R., J. H. Pryor, C.B. Ballard, C.B. Ponchirolli-Schneider, D. W. Forrest and R.A. Godke. 2008. Intracellular lipids within bovine embryos have an effect on cryotolerance and overall embryo viability post-thaw. Proc. SBTE Meetings (Brazil) (August 21-24th). 36 (Suppl. 2):319-348.

Nelida Rodriguez-Osorio. Molecular reprogramming in bovine embryos after serial somatic cell chromatin transfer. Ph.D. Thesis

Purcell, S.C. 2008. Characterization of periattachment factor. Ph.D. Dissertation, Colorado State University, Fort Collins, CO.

Seidel, G.E., Jr. 2008. Artificial insemination of cattle with sexed semen  Dealing with low numbers of slightly compromised sperm. Proc. Brazilian Embryo Transfer Society. In Press.

Seidel, G.E., Jr. 2008. Current status of sexing sperm. Proc. Italian Embryo Transfer Society, pp. 1-8.

Seidel, G.E., Jr. 2008. History of commercializing sexed semen for cattle. Proc. Italian Embryo Transfer Society, pp. 1-17.

Seidel, G.E., Jr. 2008. Pregnancy rates with embryos vitrified in 0.25-mL straws. Proc. Italian Embryo Transfer Society, pp. 1-9.

Seidel, G.E., Jr., ed. 2008. Animal Models of Reproduction. Theriogenology 69:1-67.

Smith, J.E. 2008. Anaysis of GnRH receptor gene expression in lines of swine with divergent ovulation rates. MS Thesis. University of Nebraska, Lincoln, NE.

Wall, R.J., G. Labile, E. Maga, G.E. Seidel, Jr. and B. Whitelaw. 2008. Animal productivity and genetic diversity of transgenic and cloned animals. Issue paper #XX, Council for Agricultural Service and Technology, Ames, IA, 41 pp.

Williams, K., K. Thibodeaux and R. A. Godke. 2008. Developing gamete and embryo freezing technologies using animal models. ESHRE Fertility World 8:21-23.

Youngs, C.R. and R.A. Godke. Biotechnology: Cattle Embryo Transfer Technology. 2008. In: D.R. Heldman, A. Bridges, D. Hoover, and M.B. Wheeler, Eds. Encyclopedia of Biotechnology in Agriculture and Food. Marcel Dekker, New York, NY (In press).

3. Abstracts

Alapati, R., M. Stout, R. Godke and R. Devireddy. 2008. Comparative freezing response of ejaculated and epididymal bovine spermatozoa. Proc. Bioengineering Conf. (June 25-29, 2008) Marco Island, Florida. ASME. pp.1-2.

Amorim, L.S., D.J. Walker and G.E. Seidel, Jr. 2008. Development of in vitro-produced bovine embryos cultured in sequential or continuous media. Reprod. Fertil. Develop. 20:141.

Anthony, R.V. and G.E. Seidel, Jr. 2008. Characteristics of peri-attachment factor. Proc. NRI Animal Reproduction Annual Investigator Meeting, pp. 89-91.

Ballard, C., C.R. Looney, B.R. Lindsey, J.H. Pryor, J.W. Lynn, K.R. Bondioli and R.A. Godke. 2008. Comparing oocyte lipid content with circulating cholesterol and triglyceride levels of Bos taurus and Bos indicus donor cows. Reprod. Fertil. Develop. 20:177.

Barcelo-Fimbres, M. and G.E. Seidel, Jr. 2008. Effects of embryo sex and glucose or fructose in culture media on bovine embryo development. Reprod. Fertil. Develop. 20:141-142.

Bradecamp, BE., A Snyder, A Rocha, SK Lyle, J Len, BE Eilts, RA Godke, and DL Paccamonti. 2008. Can time of day of ovulation be influenced by hCG? Pferdeheilkunde 24:112. (Abstract)

Chang CC, LY Sung, T Amano, M Amano, CJ Lin, ZP Nagy, J Xu, XC Tian, X Yang. 2008. Derivation of embryonic stem cells by nuclear transfer using cryopreserved eggs (accepted for presentation in the 41st annual meeting of the Society for the Study of Reproduction, Kailua-Kona, HI, May 27-30, 2008).

Chaubal SA, Nedambale TL, Xu J, Shaffer C, Kilmer T, Shefle A, YangX, Tian XC, and Du F. 2008. Effect of Heparin Concentration on Bovine Preimplantational Development in vitro using Sex-sorted Sperm (presented in the 2008 IETS annual meeting, Denver, CO, Jan 5-9, 2008).

Chiasson, M.K., K. R. Bondioli, R.A. Godke and G.T. Gentry. 2008. Laser-assisted zona pellucida hatching in frozen-thawed bovine embryos. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:24.

Curry, E., S. Pratt, D. Lapin, J. Gibbons. 2008. Efficacy of a commercially available post-thaw bovine semen sexing kit in both single-ovulating and hyperstimulated cows. Reprod Fertil Dev 20:210 (abst #260).

Denniston, R. S., S. Michelet, K.R. Bondioli and R. A. Godke. Principles of Embryo Cryopreservation. 2008. In: Cryopreservation in Aquatic Species. Tiersch, T. R. and P. M. Mazik, Editors. World Aquaculture Society, Baton Rouge, Louisiana. (In press).

Farin PW, Malarkey DE, Alexander JE, Farin CE. (2008). Liver and kidney function in bovine fetuses from embryos produced in vivo or in vitro. Reprod Fertil Dev 20:180 (abst #201).

Farmer WT, Farin PW, Bischoff SR, Alexander JE, Piedrahita JA, Farin CE. (2008). Detection of antisense to Igr2r (AIR) in cattle. Reprod Fertil Dev 20:81 (abst #2).

Feltrin, C., Lima, A.S., Monaco, E.; Wilson, S.M., Kim, D., Rodrigues, J.L., Wheeler, M.B. (2008). Evaluation of Different Fusion Parameters in the Reconstruction of Swine Hand-Made Cloning Embryos. Reproduction, Fertility and Development 20:29.

Flores, R., M.L. Looper, R.W. Rorie, C.R. Bailey, D.M. Hallford, S.T. Reiter and C.F. Rosenkrans, Jr. 2008. Effects of bovine somatotropin and suckling on ovarian follicles, reproductive performance and concentrations of ghrelin, GH, and IGF-I in postpartum Brahman-influenced cows. J Anim Sci 86(E-Suppl. 3):19 (Abstr. 73).

Gentry Jr., G.T., J. Saenz, W. Forbes, K. Whitehead, D. Sanders, B. Olcott J. Chenevert, K.R. Bondioli and R.A. Godke. 2008. Use of the CIDR-G device and fixed-timed artificial insemin-ation for White-tail deer. Reprod. Fertil. Develop. 20:160.

Gentry Jr., G.T., J.A. Pitchford, M. Chiasson, L.R. Gentry, K.R. Bondioli and R.A. Godke. Effect of circulating leptin levels at synchronization on subsequent fixed-timed AI pregnancy rates in crossbred beef heifers. Reprod. Fertil. Develop. (In press).

Giraldo, A.M., D.A. Hylan, C.B. Ballard, M.N. Purpera, T.G. Vaught, J.W. Lynn, R.A. Godke and K.R. Bondioli. 2008. Effect of epigenetic modifications of donor cells on subsequent chromatin remolding of cloned embryos. Biol. Reprod. 78:832-840.

Giraldo, A.M., J.W. Lynn, M.N. Purpera, R.A. Godke and K.R. Bondioli. 2008. Inhibition of DNA Methyltransferase 1 expression in bovine fibroblast cells for nuclear transfer. Reprod. Fertil. Develop. 20:95.

Giraldo. A., J.W. Lynn, M.N. Purpera, R.A. Godke and K.R. Bondioli. DNA methylation and histone acetylation patterns in cultured bovine fibroblasts for nuclear transfer. Molec. Roprod. Develop. (In press).

Godke, R.A., G. Gentry Jr. and K. R. Bondioli. 2008. Advances in cattle reproductive technologies. Proc. WBC (May 18-23) Ft Worth, Texas. ABBA. CD Series 16 pages.

Guerrero, C.A., G. Gentry, J. Saenz, K.R. Bondioli, and R.A. Godke. Birth of calves after artificial insemination with cryopreserved bovine caudal epididymal spermatozoa harvested from a postmortem bull. Reprod. Fertil. Develop. (In press).

Guerrero, C.A., J. Smith, J.W. Lynn, K.R. Bondioli, and R.A. Godke. 2008. Birth of a calf after intracytoplasmic sperm injection (ICSI) with frozen-thawed epididymal sperm from a postmortem bull. Reprod. Fertil. Develop. 20:216.

Hudson, M.R., G.E. Seidel, Jr., E.L. Squires, B.E. Spizziri, D.J. Walker and J.K. Graham. 2008. Capacitation of stallion spermatozoa evaluated by fertilization of bovine oocytes. Reprod. Fertil. Develop. 20:181-182.

Johnson-Gauff, T., B. Olcott, J. Dzakuma, K.R. Bondioli and R.A. Godke. 2008. Selecting domestic goats by morphological parameters for nonsurgical embryo procedures. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:12.

Kim C, Park J, Amano T, Carter MG, Tian XC, Yang X. 2009. Improvement of Embyonic Stem Cell Derivation Efficiency with Novel Medium, Glucose Concentration and Epigenetic Modifications Using Alternative Starting Material, Preblastocysts (to be presented in the annual meeting of 2009 IETS, San Diego, CA).

Kim C, T Amano, J Park, MG Carter, XC Tian, X Yang. 2008. Improvement of Mouse Embryonic Stem Cell Derivation Efficiency with Novel Medium, Glucose Concentration, and Epigenetic Modifications (accepted for presentation in the 41st annual meeting of the Society for the Study of Reproduction, Kailua-Kona, HI, May 27-30, 2008).

Kim, D., Monaco, E., Lima, A., Hurley, W.L., Wheeler, M.B. (2008). High-Throughput Microfluidic Technologies for Stem Cell Research. Reproduction, Fertility and Development 20:284.

Kish, S.L., J.A. Wilson, A.A. Picou, J.W. Lynn, R.A. Godke and K.R. Bondioli. Streptolysin O Permeabilized and Mitotic Extract Treated Goat Fetal Fibroblasts for Use in Nuclear Transfer. Reprod. Fertil. Dev. (In press).

Krisher, R., Lowe, J., Bailey, A., Sturek, M. (2008) Endocrine parameters and ovarian dynamics in Ossabaw swine suggest a model for polycystic ovary syndrome. Experimental Biology. Abstract 757.24.

Krisher, R.L. (2008) Components of Oocyte Quality in the Pig. Biology of Reproduction Special Issue (abst. 755).

Lima, A.S., Monaco, E., Wilson, S.M., Kim, D., Feltrin, C., Lane, S., Bionaz, M., Hurley, W.L., Wheeler, M.B. (2008). Osteogenic differentiation in vitro of porcine adult mesenchymal stem cells. Reproduction, Fertility and Development 20: 286.

Lin CJ, LY Sung, X Yang, SA Krawetz, XC Tian. 2008. Dynamic changes of nuclear halo structure of in vivo fertilized and cloned preimplantation mouse embryos (accepted for presentation in the 41st annual meeting of the Society for the Study of Reproduction, Kailua-Kona, HI, May 27-30, 2008).

Looney, C.R., J. H. Pryor, C.B. Ballard, C.B. Ponchirolli-Schneider, D. W. Forrest and R.A. Godke. 2008. Intracellular lipids within bovine embryos have an effect on cryotolerance and overall embryo viability post-thaw. Proc. SBTE Meetings (Brazil) (August 21-24th). 36 (Suppl. 2):319-348.

Looper, M.L., R.W. Rorie C.N. Person, M.D. Person T.D. Lester and C.F. Rosenkrans, Jr. 2008. Influence of endophyte-infected fescue on semen characteristics of Brahman-influenced bulls. J Anim Sci 86(E-Suppl. 3):17 (Abstr. 63).

Mocklin, C.M., SK Lyle, BE Eilts, EGH Wouters, LR Gentry, RA Godke, and DL Paccamonti. 2008. Is the individual variation in response to post-ovulatory cloprostenol in mares due in part to timing of initiation of treatment in relation to ovulation? Pferdeheilkunde 24:115. (Abstract)

Monaco, E., Lima, A.S., Wilson, S.M, Kim, D., Bionaz, M., Hurley, W.L., Wheeler, M.B. (2008) Osteopontin gene expression in immature and mature swine cumulus cells and oocytes Reproduction, Fertility and Development 20:171.

Monaco, E., Lima, A.S., Wilson, S.M, Kim, D., Bionaz, M., Hurley, W.L., Wheeler, M.B. 2008. Temporal Transcript Profiling of Porcine Adult Mesenchymal Stem Cells during Osteogenic and Adipogenic Differentiation using a 13K Porcine Microarray. International Society for Stem Cell Research. Annual Meeting. June 11-14, 2008.

Morales-Pliego, F., M. Barcelo-Fimbres, L.S. Amorim and G.E. Seidel, Jr. 2008. Optimal in vitro maturation time depends on morphological characteristics of bovine cumulus-oocyte complexes. Reprod. Fertil. Develop. 20:203-204.

Nel-Themaat, L., M. Gomez, C.E. Pope, M. Lopez, G. Wirtu, A. Cole, B.L. Dresser, L.A. Lyons, K. R. Bondioli and R.A. Godke. 2008. Cloning from semen. Part 1: In vitro proliferation of epithelial cells on embryonic fibroblasts after isolation from semen by gradient centrifugation. Cloning & Stem Cells 10:143-160.

Nel-Themaat, L., M. Gomez, C.E. Pope, M. Lopez, G. Wirtu, J.L. Jenkins, A. Cole, B.L. Dresser, K. R. Bondioli and R.A. Godke. 2008. Cloning from semen. Part 2: Intergeneric nuclear transfer of semen-derived eland (Taurotragus oryx) epithelial cells into bovine oocytes. Cloning & Stem Cells 10:161-172

Paczkowski, M.J., Bidwell, C., Waddell, J., Spurlock, D., Krisher, R.L. (2008) In vitro maturation alters gene expression in mouse oocytes. Reprod. Fertil. Dev. 20(1):82 (abst. 3).

Pant, D., Keefer, C.L. (2008) Noggin Stimulates Expression of the Pluripotency Marker Nanog in Bovine Inner Cell Mass (ICM) Derived Explants. ISSCR Annual Conference Proceedings.

Park JP, SR Davis, T Suteevun, RS Bruno, RS Prather, X Yang, XC Tian. 2008. DNA Methylation analyses in lungs of cloned pigs (accepted for presentation in the 41st annual meeting of the Society for the Study of Reproduction, Kailua-Kona, HI, May 27-30, 2008).

Pennington, P. M., L.R. Gentry, C.E. Pope, R.A. Maclean, D. L. Paccamonti, B.L.Dresser, K.R. Bondioli, R. A. Godke and G. Wirtu. 2008. Characterization of the common eland (Taurotragus orryx) estrous cycle. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:11.

Person, C.N., T.D. Lester, M.D. Person and R.W. Rorie. 2008. Effects of penicillamine, hypotaurine and epinephrine (PHE) on post-thaw bovine sperm parameters, as measured by computer-assisted sperm analysis. J Anim Sci 86(E-Suppl. 3):16 (Abstr. 61).

Picou, A.A. R. MacLean, B. Dresser, R. Godke and K. R. Bondioli. 2008. Isolation and characterization of bovine adipose derived somatic stem cells. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:4.

Picou, A.A., MacLean,R.A., Dresser,B., Godke,R.A. and Bondioli,K.R. Isolation and Characterization of Bovine Adipose Derived Somatic Stem Cells. Reprod. Fertil. Dev. (In press).

Powell, J.G., A.H. Brown, Z.B. Johnson, R.W. Rorie, C.A. Tucker, J.L. Reynolds and J.A. Hornsby,. 2008. Factors associated with pregnancy rate in first exposed, crossbred heifers. J Anim Sci 86(E-Suppl. 3):27 (Abstr. 104).

Purpera, M.N., C.B. Ballard, A.M. Giraldo, D. Hylan, R.A. Godke and K.R. Bondioli. 2008. Effects of culture medium and protein supplementation on mRNA expression of in vitro produced preimplantation bovine embryos. Reprod. Fertil. Develop. 20:173.

Rasmussen, S., Z. Brink, K. McSweeney and G. Seidel. 2008. Use of commercially available oocytes and sexed sperm for IVF/ET and AI in dairy cattle. J. Anim. Sci. 85(E-Suppl. 2):460.

Rodriguez-Melendez, R., B. White and J. Zempleni. 2008. Biotin-dependent cell signaling networks in mouse embryos. J. Nutrition (Accepted) (Abstract).

Farmer WT, Farin PW, Bischoff SR, Alexander JE, Piedrahita JA, Farin CE. (2008). Detection of antisense to Igr2r (AIR) in cattle. Reprod Fertil Dev 20:81 (abst #2).

Saenz, J.R., C. Dumas, B.L. Dresser, Martha Gomez, R, Godke and C.E. Pope. 2008. Survival and in vitro functionality of cat epididymal spermatozoa following cryopreservation in extenders with and without egg yolk. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:7.

Saenz, J.R., C. Guerrero, D. Paccamonti, B. Eilts, K. Bondioli, R.A. Godke. 2008. Processing of postmortem bovine epididymal sperm after cooling the testes for 24 hours. Reprod. Fertil. Develop 20:126.

Saenz. J.R., C. Dumas, B.L. Dresser, M.C. Gómez, R.A. Godke and C.E. Pope. Survival and in vitro functionality of cat epididymal spermatozoa following cryopreservation in extenders with or without egg yolk. Reprod. Fertil. Develop. (In press).

Schiffmacher, A., Keefer, C.L. (2008) Characterization of the bovine embryo-derived CT-1 Cell line for studying regulation of transcription factors expressed with the bovine trophectoderm. Reprod. Fert. Dev. 20(1)136-137, abst. 113.

Schiffmacher,A., Keefer, C.L. (2008) Expression of an CT4(POU5F1) Pseudogene in the Bovine Embryo Derived CT-1 Cell Line. Biol. Reprod. S1. abstr 204.

Seidel, G.E., Jr. 2008. Integrating in vitro embryo technologies into the dairy industry. Proc. NRI Animal Reproduction Annual Investigator Meeting, pp. 79-80.

Storer, W.A., D.L. Thompson Jr., K. Bondioli, R.A. Godke, A.S. Khan, P.A. Brown, R. Draghia-Akli. 2008. Evaluation of plasmid delivery by electroporation for GnRH production in stallions. Equine Vet. Sci. 28(3):149-155.

Stout, M.A., J.R. Saenz, G.T. Gentry, S.P. Leibo, K.R. Bondioli and R.A. Godke. Comparison of post-thaw values of epididymal sperm from White-tail deer that was frozen using glycerol or DMSO as the cryoprotectant. Reprod. Fertil. Develop. (In press).

Stout, M.A., J.R. Saenz, J. Chenevert, C.J. Schexnayder, K.R. Bondioli and R.A. Godke. 2008. Comparison of ejaculated and epididymal sperm parameters from the same bulls. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:22.

West, F. D., K. Pandiyan, K. R. Robbins and S. L. Stice. 2008. Enrichment and Differentiation of Germ-Like Cells from Human Embryonic Stem Cells. Georgia Stem Cell Initiative; Athens, (GA).

West, F. D., K. Pandiyan, K. R. Robbins and S. L. Stice. 2007. Enrichment of Germ-Like Cells in Human Embryonic Stem Cell Cultures. Society for the Study of Reproduction 40th Annual Meeting; San Antonio, (TX).

West, F. D., K. Pandiyan, K. R. Robbins and S. L. Stice. 2007. Human Embryonic Stem Cells Differentiate into Sperm and Eggs Precursor Cells. Georgia Life Sciences Summit; Atlanta, (GA).

West, F. D., K.i Pandiyan, K. R. Robbins and S. L. Stice. 2007. Enrichment of Germ-Like Cells in Human Embryonic Stem Cells Cultures. GTECH Industry Partners Symposium; Atlanta, (GA).

West, F. D., M.S. Natrajan, M.I. Roche-Rios, M.A. Bedell and S. L. Stice. 2008. Bone Morphogenetic Protein and KIT Ligand Signaling Enhances Human Embryonic Stem Cell Differentiation into Early Sperm Cells. Georgia Life Sciences Summit; Atlanta, (GA).

Wheeler, M.B., Hurley, W.L., Lane, S.J., Bressner, G.E., VanEtten, T., Kim, D., Lima, A.S., Monaco, E., Wilson, S.M. (2008). Risk assessment of alpha-lactalbumin transgenic pigs. Reproduction, Fertility and Development 20:311.

Williams K. A.A. Picou, S.L. Kish, A.M. Giraldo, R.A. Godke and K.R. Bondioli. 2008. Isolation and characterization of porcine adipose-derived adult stem cells. Cells, Tissues & Organs 186(3): 251-258.

Wirtu, G., C.E. Pope, R.A. MacLean, R. A. Godke, D. L. Paccamonti and B. L. Dresser. 2008. Reversal of motility loss in Bongo antelope (Tragelaphus eurycerus isaaci) spermatozoa contaminated with hyposmotic urine during electroejaculation. Anim. Reprod. Sci. 103:392-397

Williams, K., K. Thibodeaux and R. A. Godke. 2008. Developing gamete and embryo freezing technologies using animal models. ESHRE Fertility World 8:21-23.

Williams, K.J., J.M. Gimble, R.A. Godke and K.R. Bondioli. Characterization of Porcine Adipose Tissue-Derived Adult Stem Cell Surface Proteins. Reprod. Fertil. Dev. (In press).

Williams, K.J., R.A. Godke and K.R. Bondioli. 2008. Characterization of porcine adipose tissue-derived adult stem cell surface proteins. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:20.

Wilson. J.A., R.A. Godke and K.R. Bondioli. 2008. Gene copy number of transfected caprine fibroblast cells. Proc. Gulf Coast Conservation Biology Symposium (New Orleans, LA, October 2, 2008). 5:19.

Wirtu, G., C.E. Pope, D.L Paccamonti, R. A Godke, B. L Dresser. 2008. Ultrasound-guided retrieval and in vitro maturation of eland (Taurotragus oryx) and bongo (Tragelaphus eurycerus isaaci) antelope oocytes. Anim. Reprod. Sci. (Online doi:10.1016/j.anireprosci.2008.02.017).

Wirtu, G., C.E. Pope, R.A. MacLean, R. A. Godke, D. L. Paccamonti and B. L. Dresser. 2008. Reversal of motility loss in Bongo antelope (Tragelaphus eurycerus isaaci) spermatozoa contaminated with hyposmotic urine during electroejaculation. Anim. Reprod. Sci. 103:392-397

Wirtu, G., P.M. Pennington, C.E. Pope, R.A. MacLean, J. Mercado, J. Galiguis, D.L. Paccamonti, R.A. Godke and B.L. Dresser. Behavioral aspects of estrus in the common eland antelope (Taurotragus oryx). Reprod. Fertil. Develop. (In press).

Youngs, C.R. and R.A. Godke. Biotechnology: Cattle Embryo Transfer Technology. 2008. In: D.R. Heldman, A. Bridges, D. Hoover, and M.B. Wheeler, Eds. Encyclopedia of Biotechnology in Agriculture and Food. Marcel Dekker, New York, NY (In press).

Yuan, Y., Krisher, R.L. (2008) The presence of ammonium in chemically defined maturation medium inhibits porcine oocyte nuclear maturation and subsequent embryonic development in vitro. Reprod. Fertil. Dev. 20(1):208-209 (abst. 257).

4. Miscellaneous publications (semi-technical/lay publications)

Flores, R., M.L. Looper, R.W. Rorie, C.R. Bailey, D.M. Hallford, S.T. Reiter and C.F. Rosenkrans, Jr. 2008. Effects of bovine somatotropin and suckling on reproductive performance, ovarian follicles, and concentrations of ghrelin, GH, and IGF-I in postpartum Brahman-influenced cows. Ark Agri. Exp. Sta., Res. Series 563:53-55.

Rorie, R.W. and M.D. Person. 2008. Effects of a single nucleotide polymorphism in the interleukin-8 receptor on susceptibility of dairy cattle to mastitis. Ark Agri. Exp. Sta., Res. Series 563:126-127.

Seidel, G.E., Jr. 2008. Cloning. World Book Encyclopedia, pp. 682-684.

Log Out ?

Are you sure you want to log out?

Press No if you want to continue work. Press Yes to logout current user.

Report a Bug
Report a Bug

Describe your bug clearly, including the steps you used to create it.