NC_OLD229: Porcine Reproductive and Respiratory Disease: Methods for the integrated control, prevention and elimination of PRRS in United States Swine

(Multistate Research Project)

Status: Inactive/Terminating

SAES-422 Reports

12/01/2004

Refereed publications
1. Bassaganya-Riera J, Thacker B, Yu S, Strait E, Wannemuehler M, Thacker E. 2004. Impact of immunizations with porcine reproductive and respiratory syndrome virus on lymphocyte recall responses of CD8+ T cells. Viral Immunol 17:25-37.

2. Bastos RG, Dellagostini OA, Barletta RG, Doster AR, Nelson,E Zuckermann F, Osorio FA. 2004. Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus. Vaccine 22:467-474;

3. Batista L, Dee SA, Rossow KD, Polson DD, Xiao Z, Olin M, Molitor TW, Murtaugh MP, Pijoan C. 2004. Detection of porcine reproductive and respiratory syndrome virus in pigs with low positive or negative ELISA s/p ratios. Vet Rec 154:25-26.;

4. Batista L, Dee SA, Rossow KD, Xiao Z, Olin M, Molitor TW, Murtaugh MP, Pijoan C. Virological and immunological responses to porcine reproductive and respiratory syndrome virus (PRRSV) in a large population of breeding age female swine. Can J Vet Res (In press).;

5. Batista L, Pijoan C, Dee S, Olin M, Molitor T, Xiao Z, Murtaugh M. Virological and immunological features of homologous and heterologous protection to porcine reproductive and respiratory syndrome virus (PRRSV) in gilts. Can J Vet Res (In press).;

6. Batista L, Pijoan C, Ruiz A, Utrera V, Dee SA. 2004. Assessment of the transmission of Mycoplasma hyopnuemoniae by personnel. J Swine Health Prod 12:75-77.;

7. Batista L, Pijoan C, Lwamba H, Johnson CR, Murtaugh MP. 2004. Genetic diversity and possible avenues of dissemination of PRRSV in two geographic regions of Mexico. J Swine Health Prod 12:170-175.;

8. Cancel-Tirado S, Evans R, Yoon K-J. 2004. Identification of PRRSV epitopes associated with antibody-dependent enhancement and neutralization of virus infection using monoclonal antibodies. Vet Immunol Immunopathol (in press).;

9. Cha S-H, Chang C-C, Yoon K-J. 2004. Instability of ORF5 RFLP pattern of PRRSV during sequential pig-to-pig passages. J Clin Microbiol (in press).;

10. Dawson HD, Royaee AR, Nishi S, Kuhar D, Schnitzlein WM, Zuckermann F, Urban JF, Lunney JK. 2004. Identification of key immune mediators regulating T helper 1 responses in swine. Vet Immunol Immunopathol 100:105-111.;

11. Dee SA, Boorman J, Moon RD, Fano E, Trincado C, Pijoan C. 2004. Transmission of porcine reproductive and respiratory syndrome virus under field conditions during a putative increase in the fly population. J Swine Health Prod 12:242-245.;

12. Dee SA, Deen J, Burns D, Douthit G, Pijoan C. 2004. An assessment of sanitation protocols for commercial transport vehicles contaminated with porcine reproductive and respiratory syndrome virus. Can J Vet Res 68:208-214;

13. Dee SA, Deen J, Otake S, Pijoan C. 2004. An assessment of transport vehicles as a source of porcine reproductive and respiratory syndrome virus transmission to susceptible pigs. Can J Vet Res 68:124-133.;

14. Dee SA, Deen J, Pijoan C. 2004. An evaluation of four intervention strategies to prevent mechanical transmission of porcine reproductive and respiratory syndrome virus. Can J Vet Res 68:19-26.;

15. Dee SA, Deen J, Pijoan C. Evaluation of disinfectant efficacy for sanitizing porcine reporductive and respiratory syndrome virus-contaminated transport vehicles. Can J Vet Res (in press).;

16. Dee SA, Jacobson L, Rossow K, Pijoan C. A laboratory model to evaluate the role of aerosols in the transport of porcine reproductive and respiratory syndrome virus Vet Rec (in press).;

17. Dee SA, Martinez BC, Clanton CJ. Survival and infectivity of porcine reproductive and respiratory syndrome virus in swine lagoon effluent. Vet Rec (in press).;

18. Dee SA, Torremorell M, Deen J, Thompson B, Pijoan C. An evaluation of the Thermo-Assisted Drying and Decontamination (TADD) system for the elimination of porcine reproductive and respiratory syndrome virus from contaminated livestock transport vehicles. Can J Vet Res (in press).;

19. Dee SA. 2004. Elimination of porcine reproductive and respiratory syndrome virus by test and removal on 30 farms. J Swine Health Prod 12:129-133.;

20. Dongwan Y, Wootton SK, Li G, Song C, Rowland RRR. 2003. Colocalization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. J Virol 77: 12173-12193.;

21. Fang Y, Kim DY, Ropp S, Steen P, Christopher-Hennings J, Nelson EA, Rowland RRR. 2004 Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United States. Virus Res 100:229-235.;

22. Fano E, Pijoan C, Dee SA. Evaluation of aerosol transmission of a mixed infection of Mycoplasma hyopneumoniae and porcine reproductive and respiratory syndrome virus. Vet Rec (in press).;

23. Ferrin NH, Fang Y, Johnson CR, Murtaugh MP, Polson DD, Torremorell M, Gramer ML, Nelson EA. 2004. Validation of a blocking enzyme-linked immunosorbent assay for the detection of antibodies against porcine reproductive and respiratory syndrome virus. Clin Diagn Lab Immunol 11:503-514.;

24. Gerrits RJ, Lunney JK, Johnson LA, Pursel VG, Rohrer GA, Dobrinsky JR. 2004. A vision for artificial insemination and genomics to improve the global swine population. Theriogenology. (in press);

25. Goldberg TL, Lowe JF, Milburn SM, Firkins LD. 2003. Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection. Virology 317:197-207.;

26. Hermann JR, Honeyman MS, Zimmerman JJ, Thacker BJ, Holden PJ, Chang CC. 2003. Effect of dietary Echinacea purpurea on viremia and performance in porcine reproductive and respiratory syndrome virus-infected nursery pigs. J An Sci 81:2139-2144.;

27. Horter DC, Yoon K-J, Zimmerman JJ. 2004. A review of porcine tonsils in immunity and disease. Animal Health Research Reviews 4:143-155.;
28. Hyland K, Foss DL, Johnson CR, Murtaugh MP. 2004. Oral immunization to induce local and distant mucosal immunity in swine. Vet Immunol Immunopathol 102:331-340.;

29. Jiang Z, Zhou E-M, Ameri-Hahabadi M, Zimmerman JJ, Platt KB. 2003. Identification and characterization of auto-anti-idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein (GP5). Vet Immunol Immunopathol 92:125-135.;

30. Johnson W, Roof M, Vaughn E, Christopher-Hennings J, Johnson CR, Murtaugh MP. 2004. Pathogenic and immunological responses to porcine reproductive and respiratory syndrome virus (PRRSV) are related to viral load in acute infection. Vet Immunol Immunopathol 102:235-250.;

31. Kleiboeker SB, Schommer SK, Lee S-M, Watkins S, Chittick W, Polson D. Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative RT-PCR. J Vet Diagn Invest (in press).;

32. Lee C, Bachand A, Murtaugh MP, Yoo D. 2004. Differential cellular gene expression regulated by the porcine reproductive and respiratory syndrome virus GP4 and GP5 glycoproteins. Vet Immunol Immunopath 102:189-198 (Published on-line Oct 18, 2004).;

33. Lee C, Bachand A, Murtaugh MP, Yoo D. Yoo. 2004. Differential host cell gene expression regulated by the porcine reproductive and respiratory syndrome virus GP4 and GP5 glycoproteins. Vet Immunol Immunopathol 102:179-188.;

34. Lee C, Calvert JG, Welch SK, Yoo D. A DNA-launched reverse genetic system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Virology (in press);

35. Lee C, Rogan D, Erickson L, Zhang J, Yoo D. 2004. Characterization of the porcine reproductive and respiratory syndrome virus glycoprotein 5 (GP5) in stably expressing cells. Virus Res 104: 33-38.;

36. Lee S-M, Schommer SK, Kleiboeker SB. Porcine Reproductive and Respiratory Syndrome Virus Field Isolates Differ in in vitro Interferon Phenotypes. Vet Immunol Immunopathol (in press).;

37. Lemke CD, Haynes JS, Spaete R, Adolphson D, Vorwald A, Lager K, Butler JE. 2004. Lymphoid hyperplasia resulting in immune dysregulation is caused by PRRSV infection in neonatal pigs. J Immunol 172:1916-1925.;

38. Lopez OJ, Osorio FA. The role of antibodies in PRRSV protective immunity, a short survey. Vet Immunol Immunopathol (in press);

39. Lowe JF, Husmann R, Firkins LD, Zuckermann FA, Goldberg TL. Correlation of cellular immunity to porcine reproductive and respiratory syndrome virus and clinical disease during outbreaks of PRRS in commercial swine herds. J Am Vet Med Assoc (in press).;

40. Martens GW, Lunney JK, Baker JE, Smith DM. 2003. Rapid assignment of swine leukocyte antigen (SLA) haplotypes in pedigreed herds using a polymerase chain reaction based assay. Immunogenetics 55:395-401.;

41. Meier WA, Husmann RJ, Schnitzlein WM, Osorio FA, Lunney JK, Zuckermann FA. 2004. Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 102:299-314. ;

42. Miller LC, Fox JM. 2004. Apoptosis and porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol (Published on-line October 18, 2004);

43. Miller LC, Laegreid WW, Bono JL, Chitko-McKown CG, Fox JM. 2004. Interferon type-I response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Arch Virol (Published on-line May 26, 2004);

44. Nielsen HS, Liu G-P, Nielsen J, Oleksiewicz MB, Bøtner A, Storgaard T, Faaberg KS. 2003. Generation of an infectious clone of VR-2332, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus. J Virol 77:3702-3711.;

45. Nilubol D, Harris DL, Thacker BJ, Thacker EL. Duration and protection of maternally derived antibodies in pigs farrowed from PRRSV-exposed sows following killed PRRSV vaccine administration. Viral Immunol (submitted).;

46. Nilubol D, Harris DL, Thacker BJ, Thacker EL. Immune responses and protection against high and low virulence PRRSV in pigs vaccinated with modified live and killed PRRSV vaccine, either alone or in combination. Vaccine (submitted).;

47. Nilubol D, Harris DL, Thacker BJ, Thacker EL. Responses of pigs following vaccination with a commercial killed porcine reproductive and respiratory syndrome virus (PRRSV) vaccine containing interleukin-12 and a combination vaccination with modified-live and killed PRRSV vaccines. Vaccine (submitted).;

48. Nilubol D, Platt KB, Halbur PG, Torremorell M, Harris DL. 2004. The effect of a killed porcine reproductive and respiratory syndrome virus (PRRSV) vaccine treatment on virus shedding in previously PRRSV-infected pigs. Vet Microbiol 102:11-18.;

49. Olin MR, Batista L, Lee J, Xioa Z, Dee SA, Murtaugh MP, Pijoan C, Molitor TW. Gamma-delta lymphocyte response to porcine reproductive and respiratory syndrome virus. Virus Immunity (in press).;

50. Opriessnig T, Pallarés FJ, Nilubol D, Vincent AL, Thacker EL, Vaughn EM, Roof M, Halbur PG. Genomic homology of ORF 5 gene sequence between modified live vaccines and porcine reproductive and respiratory syndrome virus challenge isolates is not predictive of vaccine efficacy. J Swine Health Prod (in press).;

51. Otake S, Dee SA, Moon RD, Rossow KD, Trincado C, Pijoan C. 2004. Studies on the carriage and transmission of porcine reproductive and respiratory syndrome virus in an individual housefly (Musca domestica, Linnaeus). Vet Rec 154:80-85.;

52. Ropp SL, Fang Y, Mahlum Wees CE, Nelson EA, Rossow KD, Bien M., Arndt B, Preszler S, Steen P, Christopher-Hennings J, Collins JE, Benfield DA, Faaberg KS. 2004. Characterization of emerging European-like PRRSV isolates in the United States. J Virol 78:3684-3703.;

53. Ropp SL, Mahlum Wees CE, Fang Y, Nelson EA, Rossow KD, Bien M, Arndt B, Preszler S, Steen P, Christopher-Hennings J, Collins JE, Benfield DA, Faaberg KS. 2004. Characterization of emerging European-like PRRSV isolates in the United States. J Virol 78:3684-3703.;

54. Rowland RRR, Lawson S, Rossow K, Benfield DA. 2003. Lymphotropism of porcine reproductive and respiratory syndrome virus replication during persistent infection of pigs originally exposed to virus in utero. Vet Micro 96:219-235.;

55. Rowland RRR, Scheider P, Fang Y, Wootton S, Yoo D, Benfield DA. 2003. Peptide domains involved in the localization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus. Virology 316:135-145.;

56. Rowland RRR, Schneider P, Fang Y, Wootton S, Yoo D, Benfield DA. 2003. Peptide domains involved in the trafficking of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. Virology 316:135-145;

57. Rowland RRR, Yoo D. 2003. Nucleolar-cytoplasmic shuttling of PRRSV nucleocapsid protein: a simple case of molecular mimicry or the complex regulation by nuclear import, nucleolar localization and nuclear export signal sequences. Virus Res 95:23-33.;

58. Rowland RRR, Yoo D. 2003. Nucleolar-cytoplasmic shuttling of the PRRSV nucleocapsid protein: a simple case of molecular mimicry or the complex regulation by nuclear import, nucleolar localization and nuclear export signal sequences. Virus Res 95:23-33.;

59. Royaee AR, Husmann RJ, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann FA, Lunney JK. 2004. Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination. Vet Immunol Immunopathol 102:199-216. ;

60. Schurrer JA, Dee SA, Moon RD, Rossow KD, Mahlum C, Mondaca E, Otake S, Fano E, Collins JE, Pijoan C. 2004. Spatial dispersal of porcine reproductive and respiratory syndrome virus-contaminated flies following contact with experimentally infected pigs Am J Vet Res 65:1284-1292.;

61. Smith DM, Martens GW, Lunney JK, Ando A, Lee J-H, Ho C-S, Schook L, Renard C, Chardon P. Nomenclature for factors of the SLA Class I System. Tissue Antigens (in press).;

62. Trincado C, Dee S, Jacobson L, Pijoan C. An evaluation of an all glass impinger (AGI-30) for the detection of porcine reproductive and respiratory syndrome virus in natural and artificial aerosols. Vet Rec (in press).;

63. Trincado C, Dee SA, Jacobson L, Otake S, Rossow K, Pijoan C. 2004. Attempts to transmit porcine reproductive and respiratory syndrome virus by aerosols under controlled field conditions. Vet Rec 154:294-297.;

64. Trincado C, Dee SA, Rossow KD, Halvorson D, Pijoan C. 2004. Transmission of porcine reproductive and respiratory syndrome virus by non-porcine vectors: A re-evaluation of Mallard ducks. Vet Rec 154:233-237.;

65. Truong HM, Lu Z, Kutish G, Galeota J, Osorio FA, Pattnaik AK. 2004. A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo markers of virulence and transmissibility characteristics of the parental strain. Virology 325:308-319.;

66. Vincent A, Nilubol D, Thacker EL. Comparison of a quantitative competitive reverse transcription polymerase chain reaction (QC-RT-PCR) and realtime RT-PCR for quantitation of North American isolates of the porcine reproductive and respiratory syndrome virus. J Vet Diagn Invest (submitted).;

67. Vincent AL, Thacker BJ, Halbur PG, Rothschild MF, Thacker EL. An investigation of susceptibility to porcine reproductive and respiratory syndrome virus between two genetically diverse commercial lines of pigs. J An Sci (submitted).;

68. Vincent AL, Thacker BJ, Halbur PG, Rothschild MF, Thacker EL. The in vitro susceptibility of macrophages to porcine reproductive and respiratory syndrome virus varies between genetically diverse lines of pigs. Viral Immunol (submitted).;

69. Wasilk A, Callahan J, Christopher-Hennings J, Gay BT, Fang Y, Dammen M, Torremorell M, Polson D, Mellencamp M, Nelson EA, Nelson W. 2004. Detection of U.S. and Lelystad/European-like porcine reproductive and respiratory syndrome virus and relative quantitation in boar semen and serum by real-time PCR. J Clin Micro 42:4453-4461.;

70. Welch SK, Jolie J, Pearce DS, Koertje WD, Fuog E, Shields SL, Yoo D, Calvert JG. 2004. Construction and evaluation of genetically-engineered replication-incompetent porcine reproductive and respiratory syndrome virus vaccine candidates. Vet Immunol Immunopath 102: (Published on-line Oct 18, 2004).;

71. Wootton S, Yoo D. 2003. Homo-oligomerization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein and the role of disulfide linkages. J Virol 77:4546-4557.;

72. Xiao Z, Batista L, Dee SA, Halbur P, Murtaugh MP. 2004. The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of the virus load. J Virol 78:5923-5933.;

73. Xiao Z, Murtaugh MP. 2004. beta-Glucan enhancement of T cell IFNg response in swine. Vet Immunol Immunopathol 102:317-322.;

74. Yoo D, Lee C, Welch SK, Calvert J. 2004. Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and their potential as vaccine vectors. Vet Immunol Immunopath 102:143-154 (Published on-line Oct 19, 2004).;

75. Yoo D, Wootton S, Li G, Song C, Rowland RR. 2003. Co-localization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. J Virol 77:12173-12183.;

76. Yuan S, Murtaugh MP, Schumann FA, Mickelson D, Faaberg KS. 2004. Characterization of heteroclite subgenomic RNAs associated with PRRSV infection. Virus Res 105:75-87.;

77. Zhou E-M, Clavijo A, Jiang Z, Ameri-Mahabadi M, Zimmerman JJ. 2004. Induction of auto-anti-idiotypic antibodies specific for antibodies to matrix and envelope glycoprotein from pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 101:49-59.;

Abstracts and Proceedings
1. Bachand A, Lee C, Yoo D. 2003. The lipid-mediated membrane attachment of the PRRSV GP4 glycoprotein. Conf Res Worker in Anim Disease.;

2. Boorman J, Dee SA, Moon R, Rossow K, Otake S, Pijoan C. 2004. Spatial dispersal of PRRSV contaminated flies following contact with experimentally infected pigs. Proc Congr Int Pig Vet Soc, p. 28.;

3. Callahan JD, Christopher-Hennings J, Gay TA, Reos ME, Fang Y, Dammen M, Wasilk A, Galeota J, Osorio FA, Torremorell M, Nelson WM, Nelson EA. 2003. Development, validation and commercialization of a real-time RT-PCR assay for the detection of Lelystad, European-like and US Porcine reproductive and respiratory syndrome viruses. Proc Annu Meet Am Assoc Vet Lab Diagn, p. 142.;

4. Calzada-Nova G, Husmann R, Zuckermann FA. 2003. Porcine CD4+/CD3- peripheral blood leukocytes have plasmacytoid morphology and express SWC3  a member of the signal regulatory protein (SIRP) family. Autumn Immunology Conference, Chicago, Illinois.;

5. Calzada-Nova G, Husmann R, Zuckermann FA. 2004. Porcine CD4+/CD3- peripheral blood leukocytes have plasmacytoid morphology and express SWC3  a member of the signal regulatory protein (SIRP) family. 7th Internat Vet Immunol Symp. Quebec City, Canada.;

6. Cano JP, Dee SA, Trincado C, Polson D, Pijoan C. 2004. Impact of repeated immunization with a cloesly related vaccine (IngelVac MLV) on PRRSV VR-2332 persistence and transmission in a population of pigs. Proc AD Leman Swine Conf, p. 9.;

7. Cha S-H, Chang C-C, Yoon K-J. 2004. Characterization of genetic variation of envelope-associated protein genes of PRRSV during sequential pig-to-pig passages. Proc Annual Meet Am Soc Virol, Abstract # P24-7, p. 252.
8. Cha S-H, Yoon K-J. 2004. Instability of RFLP pattern of PRRSV during pig-to-pig passages. Proc Congr Int Pig Vet Soc 1:26.;

9. Chang C-C, Yoon K-J, Zimmerman JJ. 2003. Evolution of PRRSV during prolonged in vivo replication. Combining Proceedings of Taiwan Association of Veterinary Medicine and Animal Husbandary and Chinese Society of Veterinary Science, p 80.;

10. Chang C-C, Zimmerman JJ, Yoon K-J. 2003. Mutation of PRRSV in persistently infected pigs. Combining Proceedings of Taiwan Association of Veterinary Medicine and Animal Husbandary and Chinese Society of Veterinary Science, p. 79.;

11. Chang CC, Zimmerman JJ, Yoon K-J. 2004. Genetic and antigenic variation of PRRSV in persistently infected pigs. Proc Congr Int Pig Vet Soc 1:401.;

12. Cho J, Dee S, Guedes A, Trincado C, Rossow K, Pijoan C. 2004. Preliminary data on the concentration of PRRSV in individual pig aerosols and the patterns of shedding. Proc Congr Int Pig Vet Soc, p.30.;

13. Cho J, Dee SA, Trincado C, Guedes A, Rossow K, Pijoan C. 2004. An assessment of PRRSV concnetration and shedding patterns in 25 kg and 120 kg pigs. Proc AD Leman Swine Conf, p.10.;

14. Christopher-Hennings J, Callahan J, Gay T, Wasilk A, Fang Y, Dammen M, Torremorell M, Polson D, Mellencamp M, Nelson EA, Nelson WM. 2003. Validation of a real-time, quantitative PCR assay to detect U.S. and Lelystad/European-like PRRSV in boar semen and serum. Conf Res Worker in Anim Disease.;

15. Christopher-Hennings J, Callahan J, Wasilk A, Gay T, Fang Y, Dammen M, Torremorell M, Polson D, Mellencamp M, Nelson E, Nelson W. 2004. Development, validation, and commercialization of a real-time PCR for the detection of Lelystad, European-like and US PRRSV. Proc Annu Meet Am Assoc Swine Vet.;

16. Christopher-Hennings J, Callahan JD, Fang Y, Wasilk A, Dammen M, Gay TA, Reos ME, Nelson EA, Nelson WM. 2004. Quantification of porcine reproductive and respiratory syndrome virus in boar serum and semen. Proc Annu Meet Am Assoc Vet Lab Diagn.;

17. Dee SA, Deen J, Burns D, Douthit G, Pijoan C. 2004. An assessment of sanitation protocols for commercial transport vehicles contaminated with PRRSV. Proc Congr Int Pig Vet Soc, p. 29.;

18. Dee SA, Deen J, Otake S, Pijoan C. 2004. An experimental model to evaluate the role of transport vehicles as a source of PRRSV transmission to susceptible pigs. Proc Congr Int Pig Vet Soc, p. 27.;

19. Dee SA, Deen J, Pijoan C. 2004. Assessing the risk of transport vehicles in the transmission of PRRSV. Proc Annu Meet Am Assoc Swine Vet.;

20. Dee SA, Torremorell M, Deen J, Pijoan C. 2004. An evaluation of the Thermo-Assisted Drying and Decontamination (TADD) system for the elimination of PRRSV from contaminated livestock transport vehicles. Proc AD Leman Swine Conf, p.14.;

21. Dee SA. 2004. A critical review of the risk of local spread of PRRSV by livestock transport vehicles and insects. Proc Congr Int Pig Vet Soc, p. 814.;

22. Dee SA. 2004. An overview of PRRS in the USA. Suinitalia, Reggio Emilia, Italy.;

23. Dee SA. 2004. New information of PRRSV transmission. Memorias del XXXV Congreso Nacional, Asociación Mexicana de Veterinarios Especialistas en Cerdos. Mazatlan, Mexico.;

24. Dee SA. 2004. The future of PRRS in North America: What will the next decade bring? Proc AD Leman Swine Conf.;

25. Dee SA. 2004. The Science of PRRS: What we know (and dont know) about diagnosis, transmission and control. Proc Annu Meet Am Assoc Swine Vet.;

26. Dee SA. 2004. Validation of the Thermo-Assisted Drying and Decontamination (TADD) system. Memorias del XXXIX Congreso Nacional, Asociación Mexicana de Veterinarios Especialistas en Cerdos. Mazatlan, Mexico.;

27. Dee SA. 2004. Validation of Thermo-Assisted Drying and Decontamination. PIC Swine Health Symposium, Nashville, TN.;

28. Fang Y, Kim D, Rowland RRR, Ropp S, Steen P, Christopher-Hennings J, Nelson EA. 2003. Heterogeneity in the NSP2 gene of European-like PRRSV isolated in the United States. Conf Res Worker in Anim Disease.;

29. Fang Y, Zhang WP, Rowland RRR, Christopher-Hennings J, Nelson EA. 2003. Phylogeny of European-like PRRSV isolates in North America. Conf Res Worker in Anim Disease.;

30. Fano E, Pijoan C, Dee S. 2004. Aerosol transmission of a mixed infection of Mycoplasma hyopneumoniae and PRRSV under field conditions. Proc Congr Int Pig Vet Soc, p. 185.;

31. Fano E, Pijoan C, Dee S. 2004. PRRSV persistence in both directly challenged and contact-control pigs. Proc Congr Int Pig Vet Soc, p. 54.;

32. Galina-Pantoja L, Solano-Aguilar GI, Mellencamp MA, Bastiaansen J, Lunney JK. 2004. Relationship between immune cells and pig growth on a commercial farm. Proc Congr Int Pig Vet Soc 1:381.;

33. Han J, Mattila TL, Schumann F, Liu G, Faaberg KS. 2004. Effect of heteroclite subgenomic mRNA on porcine reproductive and respiratory syndrome virus infectious clone progeny growth. Proc Annual Meet Am Soc Virol, Abstract #W17-11.;

34. Hermann JR, Evans R, Roof M, Burkhart K, Zimmerman J. 2004. Estimate of infectious dose 50 in young pigs orally exposed to PRRSV. Proc Am Soc An Sci Midwest Branch Meetings, Des Moines, Iowa. Abstract 85.;

35. Hermann JR, Evans RB, Hoff SJ, Yoon KJ, Zimmerman J. 2003. Effect of exposure to commercial antifoams on the viability of three continuous cell lines. Conf Res Worker in Anim Disease, Abstract 20.;

36. Hermann JR, Evans RB, Hoff SJ, Zimmerman J. 2003. Effect of exposure to commercial antifoams on the viability of three continuous cell lines. Proc Annu Meet Am Assoc Vet Lab Diagn, pp. 154.;

37. Hermann JR, Evans RB, Roof M, Burkhart K, Zimmerman J. 2003. Transmission of PRRSV to young swine by oral exposure. Conf Res Worker in Anim Disease, Abstract 142.;

38. Hermann JR, Evans RB, Roof M, Burkhart K, Zimmerman JJ. 2004. Transmission of PRRSV to young swine by oral exposure. Proc Annu Meet Am Assoc Swine Vet, p. 413.;

39. Hermann JR, Kaiser M, Roof M, Burkhart K, Zimmerman J. 2004. Infectious dose of young pigs experimentally inoculated with PRRSV by oral and intranasal routes. Proc Am Soc An Sci Joint Annual Meetings Vol 82 (Suppl 1), Abstr #719.;

40. Hodgins DC, Yoo D, Shewen PE, Babiuk LA. 2003. Systemic and mucosal antibody responses of neonatal calves vaccinated with a recombinant bovine adenovirus expressing the HE protein of bovine coronavirus. Conf Res Worker in Anim Disease.;

41. Karriker L. 2004. Nuevas tendencies en el diagnóstico de PRRS. Symposium Internacional de Porcinocultura, XXXVII Semana Nacional de Ganado Porcino (SEPOR 2004). Lorca (Murcia) Spain, pp. 67-71.;

42. Karriker L. 2004. PRRSV Update. Proc Annual Meet Iowa Vet Med Assoc, pp. 55-60.;

43. Karriker L. 2004. PRRSV: Applying epidemiology. Proc Annu Meet Am Assoc Swine Vet, pp. 7-10.;

44. Lee C, Calvert JG, Welch SK, Yoo D. 2004. A DNA-launched reverse genetic system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Proc Annual Meet Am Soc Virol, PQ.;

45. Liu G, Nielsen HS, Zhiming O,Faaberg KS. 2003. Infectivity of porcine reproductive and respiratory syndrome virus genomic clones. Proc Annual Meet Am Soc Virol, 14-5.;

46. Lunney JK, Royaee A, Husmann R, Calzada-Nova G, Schnitzlein W, Zuckermann FA. 2004. Immunity to vaccination with PRRSV: Porcine lymphoid cell cytokine and immune gene expression profiles. Proc Am Assoc Immunol.;

47. McCaw MB, Murtaugh M, Laster SA, Roberts J, Erickson GA. 2004. PRRSv rORF specific ELISA antibody responses following repeated homologous wild-type virus challenges. Proc Congr Int Pig Vet Soc 1:36.;

48. Miller LC, Chitko-McKown CG, Bono JL, Laegreid WW, Fox JM. 2003. Identification of responsive genes in PRRSv-infected pulmonary macrophages using semi-quantitative real-time reverse transcript PCR (RT-PCR). Conf Res Worker in Anim Disease, Abstract 155.;

49. Miller LC, Fox JM, Bono JL, Chitko-McKown CG, Laegreid WW. 2004. Early innate immune response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Proc 7th Internat Symp Positive-Strand RNA Viruses, Paper #P2-G1.;

50. Miller LC. 2004. Identification of responsive genes in PRRSv-infected cells using semi-quantitative real-time PCR. Intercampus Virology Symposium, Nebraska City, NE.;

51. Mondaca-Fernandez E, Morrison R, Dee S, Deen J, Davies P. 2004. Area-based prevalence of PRRSV and the initiation of a regional control program. Proc Congr Int Pig Vet Soc, p.104.;

52. Mondaca-Fernandez F, Morrison R, Murtaugh M. 2004. Using spatial analysis and sequencing to assess regional epidemiology of PRRSV. Proc Congr Int Pig Vet Soc 1:105.;

53. Murtaugh MP, Johnson CR, Xiao Z, Fuentes M, Yu W, Johnson W, Roof M. 2004. Comparative antibody responses to virulent and attenuated strains of PRRSV. Proc Congr Int Pig Vet Soc 1:22.;

54. Murtaugh MP, Xiao Z, Dee SA, Kleiboeker S, Roof M. 2004. Review: immunity to PRRSV is highly atypical. Proc Congr Int Pig Vet Soc 1:21.

55. Murtaugh MP. 2004. PRRS immunology: what are we missing? Proc Annu Meet Am Assoc Swine Vet, pp. 359-367.;

56. Murtaugh MP. 2004. PRRSV immunology research and the swine veterinarian. Proc Congr Int Pig Vet Soc 2:812.;

57. Nilubol D, Harris H, Thacker B, Thacker E. 2004. Duration and protection of maternally derived antibodies in pigs farrowed from PRRSV-exposed sows following killed PRRSV vaccine administration (Part 2). Proc Congr Int Pig Vet Soc 1:112.;

58. Nilubol D, Harris H, Thacker B, Thacker E. 2004. Evaluation of antibody responses in PRRSV-exposed sows, their colostrum and 3 to 4 day-old piglets following the use of killed PRRSV vaccine (Part 1). Proc Congr Int Pig Vet Soc 1:112.;

59. Nilubol D, Harris H, Thacker B, Thacker E. 2004. Survey of PRRSV RNA and neutralizing antibodies in colostrum and 3 day-old piglets farrowed from PRRSV-exposed sows. Proc Congr Int Pig Vet Soc 1:218;

60. Nilubol D, Hipple K, Vincent A, Strait E, Kitikoon P, Yu S, Thacker B, Thacker E. 2003. Comparison of anti-porcine, anti-human and anti-bovine interleukin-4 in detection of porcine IL-4 by ELISPOT and flow cytometry. Conf Res Worker in Anim Disease.;

61. Nilubol D, Thacker BJ, Harris DL, Thacker EL. 2003. PRRSV vaccination study: interleukin-12 to enhance killed vaccine immunity and combined vaccination with modified live and killed vaccines. Proc 4th Internat Symp Emerging and Re-emerging Pig Diseases, pp. 113.;

62. Pattnaik AK, Truong HM, Galeota J , Osorio FA. 2004. A highly pathogenic PRRSV generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus. Proc Annual Meet Am Soc Virol, Abstract #W17-18, p. 109;

63. Rowland RRR, Nietfeld, Dritz S. 2004. Unique aspects of in utero PRRSV infection: implications for diagnosis of the virus-infected fetus. Kansas State Swine Day;

64. Schurrer JA, Dee SA, Moon RD, Pijoan C. 2004. The influence of environmental temperature on PRRSV retention in houseflies. Proc AD Leman Swine Conf, p.16.;

65. Schurrer JA, Dee SA, Moon RD. 2004. An evaluation of intervention strategies for the control of insects on commercial swine farms. Proc AD Leman Swine Conf, p.18.;

66. Thacker E, Kittikoon K, Vincent A, Hipple K, Nilubol D, Yu S, Janke B, Thacker B. 2003. Influence of PRRSV infection on swine influenza vaccine efficacy. Proc 4th Internat Symp Emerging and Re-emerging Pig Diseases, pp. 281.;

67. Trincado C, Dee S, Otake S, Pijoan C. 2004. An assessment to determine the effectiveness of all-glass impinger (AGI) to detect varying concentrations of PRRSV in artificial aerosols. Proc Congr Int Pig Vet Soc, p.93.;

68. Trincado C, Dee S, Otake S, Pijoan C. 2004. An attempt to detect PRRSV contaminated aerosols in confined air space. Proc Congr Int Pig Vet Soc, p.31.;

69. Truong HM, Lu Z, Kutish, G, Osorio FA Pattnaik AK 2004. Infectious PRRSV from transcripts of a full-length cDNA clone(Abstract W17-10), Proc Annual Meet Am Soc Virol #W17-10, p. 109;

70. Vincent A, Thacker B, Halbur P, Rothschild M, Thacker E. 2003. An investigation of susceptibility to porcine reproductive and respiratory syndrome virus between genetically diverse lines of pigs. Conf Res Worker in Anim Disease.;

71. Waldner D, Zeman D, Kasuske A, Ropp S, Arndt B, Nelson E, Benfield D. 2003. Lymphoid tissues may represent not only sites of persistence but early sites of replication of porcine reproductive and respiratory syndrome (PRRS) virus. Conf Res Worker in Anim Disease.;

72. Waldner D, Zeman D, Kasuske A, Ropp S, Arndt B, Nelson E, Fang Y, Benfield D. 2003. Temporal studies on the replication of PRRSV in conventional pigs from six hours to 126 days post-inoculation. Proc Annu Meet Am Assoc Vet Lab Diagn, p. 141.;

73. Wesley RD, Lager KM, Kehrli Jr, ME. 2004. PRRSV infection stimulates an immediate serum interferon-gamma response. Proc Congr Int Pig Vet Soc, p. 90.;

74. Yoo D, Lee D, Welch SK, Cavert JG. 2004. Reverse genetic for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Proc 7th Internat Symp Positive-Strand RNA Viruses.;

75. Yoo D, Li G, Lee C. 2003. Interactions of the host cell protein fibrillarin with the nucleocapsid protein of nidoviruses, PRRSV and SARS coronavirus. Conf Res Worker in Anim Disease.;
76. Yoo D, Wootton S, Song C, Rowland RR. 2003. Colocalization and interaction of the PRRSV nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. IX International Symposium on Nidoviruses. Egmond aan Zee, The Netherlands.;

77. Yoo D, Wootton S, Song C. 2003. Interactions of the PRRSV nucleocapsid protein with nucleolar antigens, fibrillarin and nucleolin. Proc Annual Meet Am Soc Virol.;

78. Yoo D, Wootton S. 2003. Homo-oligomerization and the role of disulfide linkage of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. IX International Symposium on Nidoviruses. Egmond aan Zee, The Netherlands. May 24-29,;

79. Zhang X, Schreiber N, Murtaugh MP, Rutherford MS. 2004. Suppression of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection by RNA-Mediated Gene Silencing. Proc Annual Meet Am Soc Virol.;

80. Zhou EM, Mahabadi A, Jiang ZJ, Zimmerman J. 2004. Anti-idiotype induced reduction of PRRSV infection. Proc Congr Int Pig Vet Soc 1:387.

81. Zimmerman J, Benfield D, Christopher-Hennings J, Dee S, Stevenson G. 2004. Porcine reproductive and respiratory syndrome. Proc PORK Academy, Des Moines, Iowa (unnumbered pages).;

82. Zimmerman J. 2004. Aspectos epidemiológicos para el control de las enfermedades del cerdo. AMVEC Peninsular I Congresso. Telchac, Yucatán, México (unnumbered pages).;

83. Zimmerman J. 2004. Avances sobre epidemiología de PRRS. Symposium Internacional de Porcinocultura, XXXVII Semana Nacional de Ganado Porcino (SEPOR 2004). Lorca (Murcia) Spain, pp. 47-50.;

84. Zimmerman J. 2004. Nuevas tendencias en erradicación de PRRS. Symposium Internacional de Porcinocultura, XXXVII Semana Nacional de Ganado Porcino (SEPOR 2004). Lorca (Murcia) Spain, pp. 51-54.;

85. Zuckermann FA, Schnitzlein W, Husmann R, Calzada-Nova G. 2003. Association of porcine interleukin-12 receptor beta2 (IL-12Rbeta2) gene alleles with the development of high or low interferon-g response to PRRSV. Conf Res Worker in Anim Disease.;

86. Zuckermann FA. 2003. The PRRS Conundrum. University of Illinois, College of Veterinary Medicine 84th Annual Fall Conference..;

87. Zuckermann FA. 2004. Regulation of the IL-12 receptor expression and the interferon gamma response of swine to allogeneic and viral antigens. 7th Internat Vet Immunol Symp. Quebec City, Canada.;

88. Zuckermann FA. 2004. Ultimos avances sobre inmunologia frente a PRRS. Symposium Internacional de Porcinocultura, XXXVII Semana Nacional de Ganado Porcino (SEPOR 2004). Lorca (Murcia) Spain, pp. 95-103.;

Book chapters or monographs
1. Bannerman D, Paape M.J, Lunney JK. 2004. Immunity: Innate. In: Encyclopedia of Animal Science. Pond WG, Bell AW (eds). Marcel Dekker, Inc., New York (in press).;

2. Lunney JK, Paape MJ, Bannerman D. 2004.Immunity: Acquired. In: Encyclopedia of Animal Science. Pond WG, Bell AW (eds). Marcel Dekker, Inc., New York (in press).;

3. Lunney JK. 2003. Are there immune gene alleles that determine whether a pig will be healthy? In: Genetics of Pig Health Symposium. Boggess M (ed). National Pork Board Press, Des Moines, Iowa, p. 63-72.;

4. Zimmerman J, Benfield D, Murtaugh M, Osorio F, Stevenson G,
Torremorell M. Porcine reproductive and respiratory syndrome virus. In: Diseases of Swine (9th edition). Straw BE, D'Allaire S, Zimmerman J, Taylor DJ (eds). Blackwell Publishing Company, Ames Iowa (in press).;

12/15/2006

12/15/2006

Please see attached publications report

11/11/1111

07/15/2008

Please refer to the attached file for Publications.

11/11/1111

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