06/01/2015

A. Improve boar performance… (IL; MO; MS)

1. Daigneault B, McNamara K, Purdy P, Krisher RL, Knox RV, Miller DJ. 2014. Novel and traditional traits of frozen-thawed porcine sperm related to in vitro fertilization success. Theriogenology 82:266-273.

2. Daigneault B, McNamara K, Purdy P, Krisher RL, Knox RV, Rodriguez-Zas SL, Miller DJ. 2015. Enhanced fertility prediction of cryopreserved boar sperm using novel sperm function assessment. Andrology. DOI:10.1111/andr.12035.

3. Feugang JM, Liao SF, Crenshaw MA, Clemente H, Willard ST, and Ryan PL. 2015. Lectin-Functionalized Magnetic Iron Oxide Nanoparticles for Reproductive Improvement. JFIV Reprod Med Genet 3:2.

4. Feugang JM, Rodriguez-Munoz JC, Dillard DS, Crenshaw MA, Willard ST, and Ryan PL. 2015. Beneficial effects of relaxin on motility characteristics of stored boar spermatozoa. Reprod Biol Endocrinol 13:24

5. Gonzalez-Pena Fundora, D., R. Knox, J. Pettigrew, and S. L. Rodriguez-Zas. 2014. Impact of pig insemination technique and semen preparation on profitability. J. Anim. Sci. 92:72-84.

6. Knox, R., J. Salak-Johnson, M. Hopgood, L. Greiner, and J. Connor. 2014. Effect of day of mixing gestating sows on measures of reproductive performance and animal welfare. J. Anim. Sci. 92:1698-707.

7. Knox, R.V. 2014. Impact of swine reproductive technologies on pig and global food production. In: Advances in Experimental Medicine and Biology 752:131-60.

8. Knox, R.V. and B.M. Yantis. 2014. The effect of numbers of frozen-thawed boar sperm and addition of prostaglandin F2? at insemination on fertility in pigs. Anim. Reprod. Sci. 151:194-200.

9. Knox, R.V., J.M. Ringwelski, K.A. McNamara, M. Aardsma, and M. Bojko. The effect of extender, method of thawing and duration of storage on in-vitro fertility measures of frozen-thawed boar sperm. Theriogenology (epub ahead of press).

10. Petruska P, Capcarova M, Sutovsky P (2014) Antioxidant supplementation and purification of semen for improved artificial insemination in livestock species. Turkish J Vet Anim Sci, 38:643-652. Special Issue on Recent Advances in Animal Reproduction, Keith Inskeep & Robert Dailey Editors,

11. Sutovsky P (2015) New approaches to boar semen evaluation, processing and improvement. Reprod. Dom. Anim. In press.

B. Improved sow and gilt performance… (IA; IL; MO; NC; SD)

1. Clapper, J. and Paulson, C. 2015. Effects of short term administration of genistein on hypothalamic and anterior pituitary hormones in ovariectomized gilts. Open Journal of Animal Sciences. 5:163-173.

2. Bohrer, B., Flowers, W.L., Kyle, J.M., Johnson, S.S., King, V.L., Spruill, J.L., Thompson, D.P., Schroeder, A.L., and Boler, D.D. 2014. Effect of gonadotropin releasing factor (GnRF) suppression with an immunological on growth performance, estrus activity, carcass characteristics, and meat quality of market gilts. J. Anim. Sci. 92, 4719-4724.

3. Cruzen, S.M., Boddicker, R.L., Graves, K., Johnson, T.P., Arkfeld, E.K., Baumgard, L.H., Ross, J.W., Safranski, T.J., Lucy, M.C., Lonergan, S.M. 2015 Carcass composition of market weight pigs subjected to heat stress in utero and during finishing. (Accepted: Journal of Animal Science).

4. Johnson, J.S., Sanz-Fernandez, M.V., Patience, J.F., Ross, J.W., Gabler, N.K., Lucy M.C., Safranski, T.J., Rhoads, R.P., Baumgard, L.H. 2015 Effects of in utero heat stress on postnatal body composition in pigs: I. Growing Phase. Journal of Animal Science; 93:71-81.

5. Johnson, J.S., Sanz-Fernandez, M.V., Gutierrez, N.A., Patience, J.F., Ross, J.W., Gabler, N.K., Lucy M.C., Safranski, T.J., Rhoads, R.P., Baumgard, L.H. 2015 Effects of in utero heat stress on postnatal body composition in pigs: II. Finishing Phase. Journal of Animal Science; 93:82-92.

6. Boddicker, R.L., Siebert, J.T., Johnson, J.S., Pearce, S.C., Selsby, J.T., Gabler, N.K., Lucy, M.C., Safranksi, T.J., Rhoads, R.P., Baumgard, L.H., Ross, J.W. 2015 Gestational heat stress alters postnatal offspring body composition indices and metabolic parameters in pigs. PLOS One 9:e110859.

7. Knox, R.V., C.F. Shipley, G.E. Bressner, and V.L. Jarrell. 2014. Mortality, morbidity, and fertility after accidental electrical shock in a swine breeding and gestation barn. J. Swine Health Prod. 22:300-305.

8. Knox, R.V., J.N. Taibl, S.M. Breen, M.E. Swanson, and S.K. Webel. 2014. Effects of altering the dose and timing of triptorelin when given as an intravaginal gel for advancing and synchronizing ovulation in weaned sows. Theriogenology 82:379-86.

9. Johnson, J.S., M.V. Sanz Fernandez, N.A. Gutierrez, J.F. Patience, J.W. Ross, N.K. Gabler, M.C. Lucy, T.J. Safranski, R.P. Rhoads, L.H. Baumgard. Effects of in utero heat stress on postnatal body composition in pigs: I. Growing phase. 2015. J. Anim.Sci. 93: 71-81.

10. Johnson J.S., M.V. Sanz Fernandez, J.F. Patience, J.W. Ross, N.K. Gabler, M.C. Lucy, T.J. Safranski, R.P. Rhoads, L.H. Baumgard. 2015. Effects of in utero heat stress on postnatal body composition in pigs:II. Finishing phase. J. Anim. Sci. 93:82-92.

11. Boddicker R.L., J.T. Seibert, J.S. Johnson, S.C. Pearce,J.T. Selsby, N.K. Gabler, M.C. Lucy, T.J. Safranski, R.P. Rhoads, L.H. Baumgard, J.W. Ross. 2014. Gestational heat stress alters postnatal offspring body composition indices and metabolic parameters in pigs. PLoS ONE Article number e110859

12. S. M. Cruzen, R. L. Boddicker, K. Graves, T. P. Johnson, E. K. Arkfeld, L. H. Baumgard, J. W. Ross, T. J. Safranski, M. C. Lucy, S. M. Lonergan. Carcass composition of market weight pigs subjected to heat stress in utero and during finishing. J. Anim. Sci. (In press).

13. Jay S. Johnson, M. Victoria Sanz Fernandez, Jacob T. Seibert, Jason W. Ross, Matthew C. Lucy, Tim J. Safranski, Theodore H. Elsasser, Stanislaw Kahl, Robert P. Rhoads, Lance H. Baumgard. In utero heat stress increases postnatal core body temperature in pigs. J. Anim. Sci. (In press).

C. Increase the basic knowledge… (IA; IL; IN; MO; MS; NE; USDA/ARS-MUSMARC)

1. Desaulniers, A.T., R.A. Cederberg, G.A. Mills, J.J. Ford, C.A. Lents, and B.R. White. 2015. LH-independent testosterone secretion is mediated by the interaction between GnRH2 and its receptor within porcine testes. Biol. Reprod. July 1, 2015, doi:10.1095/biolreprod.115.128082.

2. Feugang JM, Greene JM, Sanchez-Rodriguez HL, Stokes JV, Crenshaw MA, Willard ST, and Ryan PL. 2015. Profiling of relaxin and its receptor proteins in boar reproductive tissues and spermatozoa. Reprod Biol Endocrinol; 13(1):1-15

3. Feugang JM, Youngblood RC, Greene JM, Willard ST, and Ryan PL. 2015. Self-illuminating quantum dots for non-invasive bioluminescence imaging of mammalian gametes. J Nanobiotechnology; 13(1):38

4. Geisert R.D., Lucy, M.C., Whyte, J.J. Ross, J.W., Mathew, D.J. 2014 Cytokines from the pig conceptus: Roles in conceptus development in pigs. Journal of Animal Science and Biotechnology; 5:51.

5. Geisert RD, Johnson GA, Burghardt RC. 2015. Implantation and Establishment of Pregnancy in the Pig. In: Regulation of Implantation and Establishment of Pregnancy in Mammals: Tribute to 45 Year Anniversary of Roger V. Short’s “Maternal Recognition of Pregnancy”. Eds. R.D. Geisert and F.W. Bazer. Advances in Anatomy, Embryology and Cell Biology, Springer, (In press).

6. Geisert RD, Lucy MC, Whyte, Ross JW, Mathew DJ. 2014. Cytokines from the pig conceptus: Roles in conceptus development in pigs. Journal of Animal Science and Biotechnology 5:51

7. Geisert RD. 2015 Introduction. In: Regulation of Implantation and Establishment of Pregnancy in Mammals: Tribute to 45 Year Anniversary of Roger V. Short’s “Maternal Recognition of Pregnancy”. Eds. R.D. Geisert and F.W. Bazer. Advances in Anatomy, Embryology and Cell Biology, Springer, (In press).

8. Hamm, J., K. Tessanne, C.N. Murphy, R.S. Prather. 2014. Transcriptional regulators TRIM28, SETDB1 and TP53 are aberrantly expressed in porcine embryos produced by in vitro fertilization in comparison to in vivo derived and nuclear transfer derived embryos. Molecular Reproduction and Development 81:552-566. doi: 10.1002/mrd.22324. Epub 2014 Apr 16. PMID: 24659575.

9. Lee, K., C. Wang, L.D. Spate, C.N. Murphy, R.S. Prather, Z. Machaty. 2014. Gynogenetic activation of pig oocytes. Cellular Reprogramming 16:121-129. doi: 10.1089/cell.2013.0074. PMID: 24661186

10. Lee, K., J. Teson, K. Whitworth, L.D. Spate, K.-W. Park, C.N. Murphy, R.S. Prather. 2014. Dynamics of the TET family in porcine preimplantation embryos is related to the zygotic genome. Developmental Biology 386: 86-95. Dx.doi.org/10.1016/j.ydbio.2013.11.024. PMID 24315853.

11. Lee, K., Wang, C., Spate, L., Murphy, C.N., Prather, R.S., Machaty, Z. 2014. Gynogenetic activation of porcine oocytes. Cell Reprogram 16:121-129.

12. Lents CA, Cushman RA, Freking BA. 2014. Measures of the ovaries and uterus during development of gilts selected for differences in uterine capacity. Journal of Animal Science 92(6):2433-2439.

13. Machado S, Kadirvel G, Daigneault B, Korneli C, Miller P, Bovin N, Miller DJ. 2014. LewisX-containg glycans on the porcine oviductal epithelium contribute to formation of the sperm reservoir. Biol Reprod 91:140, 1-9 DOI 10.1095/biolreprod.114.119503.

14. Mao, J., M.-T. Zhao, K.M. Whitworth, L.D. Spate, E.M. Walters, C. O’Gorman, K. Lee, M.S. Samuel, C.N. Murphy, R.S. Prather. 2015. Oxamflatin treatment enhances cloned porcine embryo development and nuclear reprogramming. Cellular Reprogramming 17:28-40. doi: 10.1089/cell.2014.0075. Epub 2014 Dec 30. PMID: 2548976

15. Mathew DJ, Newsom EM, Guyton JM, Tuggle CK, Geisert RD, Lucy MC. 2015. Activation of the transcription factor nuclear factor-kappa B in uterine luminal epithelial cells by interleukin 1 Beta 2: a novel interleukin 1 expressed by the elongating pig conceptus. Biology Reproduction 92 (4) 107, 1-13.

16. Miller DJ. 2015. Regulation of sperm function by oviduct fluid and epithelium. Reprod Domestic Anim (Suppl). Accepted.

17. Mtango NR, Latham KE, Sutovsky, P. (2014) Deubiquitinating enzymes in oocyte maturation, fertilization and preimplantation embryo development. In: Sutovsky, P., Editor, Posttranslational Modifications in the Reproductive System, Springer Science +Business Media LLC, Adv Exp Med Biol. 2014;759:89-110

18. Nteeba, J., Sanz-Fernandez, M.V., Rhoads, R.P., Baumgard, L.H., Ross J.W., Keating, A.F. 2015 Heat stress alters ovarian insulin mediated phosphatidylinositol-3 kinase and steroidogenic signaling in gilt ovaries. Biology of Reproduction [Epub ahead of print].

19. Regulation of Implantation and Establishment of Pregnancy in Mammals: Tribute to 45 Year Anniversary of Roger V. Short’s “Maternal Recognition of Pregnancy”. 2015. Eds. RD Geisert and FW Bazer. Advances in Anatomy, Embryology and Cell Biology, Springer, (In press).

20. Sargus-Patino CN, Wright EC, Plautz SA, Miles JR, Vallet JL, Pannier AK. 2014. In vitro development of preimplantation porcine embryos using alginate hydrogels as a three-dimensional extracellular matrix. Reproduction, Fertility and Development 26(7):943-953.

21. Schatten, H., Q.-Y. Sun, R.S. Prather. 2014. The impact of mitochondrial function/dysfunction on IVF and new treatment possibilities for infertility. Reproductive Biology and Endocrinology. 12:111.

22. Silva E, Kadirvel G, Jiang R, Bovin N, Miller DJ. 2014 Multiple proteins from ejaculated and epididymal porcine spermatozoa bind glycan motifs found in the oviduct. Andrology 2:763-771. (DOI: 10.1111/j.2047-2927.2014.00249.x).

23. Spate, L.D., A. Brown, B.K. Redel, K.M. Whitworth, C.N. Murphy, R.S. Prather. 2014. Dickkopf-related protein 1 inhibits the WNT signaling pathway and improves pig oocyte maturation. PLoS One. doi: 10.1271/journal.pone.0095114. PMID: 24739947

24. Vallet JL, Rempel LA, Miles JR, Webel SK. 2014. Effect of essential fatty acid and zinc supplementation during pregnancy on birth intervals, neonatal piglet brain myelination, stillbirth, and preweaning mortality. Journal of Animal Science 92(6):2422-2432.

25. Yi Y-J, Sutovsky M, Song W-H, Sutovsky P (2014) Protein deubiquitination during oocyte maturation influences sperm function during fertilization, anti-polyspermy defense and embryo development. Reprod. Fert. Dev., 759:89-110.

26. Zimmerman S, Yi Y-J, Sutovsky M, van Leeuwen F, Conant G, Sutovsky P (2014) Identification and characterization of RING-finger ubiquitin ligase UBR7 in mammalian spermatozoa. Cell Tissue Res. 356:261-278.

D. Increase the utilization of the rapidly advancing technology… (IN; MO; USDA/ARS-USMARC).

1. Andersson L, Archibald AL, Bottema CD, Brauning R, Burgess SC, Burt DW, Casas E, Cheng HH, Clarke L, Couldrey C, Dalrymple BP, Elsik CG, Foissac S, Giuffra E, Groenen MA, Hayes BJ, Huang LS, Khatib H, Kijas JW, Kim H, Lunney JK, McCarthy FM, McEwan JC, Moore S, Nanduri B, Notredame C, Palti Y, Plastow GS, Reecy JM, Rohrer GA, Sarropoulou E, Schmidt CJ, Silverstein J, Tellam RL, Tixier-Boichard M, Tosser-Klopp G, Tuggle CK, Vilkki J, White SN, Zhao S, Zhou H. 2015. Coordinated international action to accelerate genome-to-phenome with FAANG, the Functional Annotation of Animal Genomes project. Genome Biology 16(1):57.

2. Du Z.Q., Eisley, C.J., Onteru, S.K., Madsen, O., Groenen, M.A.M., Ross J.W., Rothschild M.F. 2014 Identification of species-specific novel transcripts in pig reproductive tissues using RNA-seq. Animal Genetics 45:198-204.

3. Gu T., Zhu, M.J.,Schroyen, M., Qu, L., Nettleton D., Kuhar, D., Lunney, J.K., Ross J.W., Zhao, S.H., Tuggle, C.K. 2014 Endometrial gene expression profiling in pregnant Meishan and Yorkshire pigs during peri-implantation. BMC Genomics 15:156.

4. Hale B.J., Yang C.X., Ross J.W. 2014 Small RNA Regulation of Reproductive Function. Molecular Reproduction and Development; 81:148-5.

5. Hale, B.J., Keating A.F., Yang, C.X., Ross J.W. 2015 Small RNAs: Their possible roles in reproductive failure. In: The Male Role in Pregnancy Loss and Embryo Implantation Failure. Ed. Richard Bronson. Springer Publishing.

6. Li, G., Q. Jia, J. Zhao, X. Li, M. Yu, M.S. Samuel, S. Zhao, R.S. Prather, C. Li. 2014. Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets. BMC Genomics 15:811. Sep 24;15:811. doi: 10.1186/1471-2164-15-811. PMID: 25253444.

7. Pirro, V., Oliveri, P., Ferreira, C.R., González-Serrano, A.F., Machaty, Z., Cooks, R.G. 2014. Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion. Anal Chim Acta. 848:51-60.

8. Prather, R.S., B.K. Redel, K.M. Whitworth, M.-T. Zhao. 2014. Genomic profiling to improve embryogenesis in the pig. Animal Reproduction Science DOI information: 10.1016/j.anireprosci.2014.04.017. PMID 24878355

9. Schneider JF, Nonneman DJ, Wiedmann RT, Vallet JL, Rohrer GA. 2014. Genomewide association and identification of candidate genes for ovulation rate in swine. Journal of Animal Science 92(9):3792-3803.

10. Whitworth, K.M., K. Lee, J.A. Benne, B.P. Beaton, L.D. Spate, S.L. Murphy, M.S. Samuel, J. Mao, C. O’Gorman, E.M. Walters, C.N. Murphy, J.P. Driver, A. Mileham, D. McLaren, K.D. Wells, R.S. Prather. 2014. Use of the CRISPR/Cas9 system to produce genetically engineered pigs from in vitro derived oocytes and embryos. Biology of Reproduction. doi: 10.1095/biolreprod.114.121723. Epub 2014 Aug 6. 91:78, 1-13. PMID: 25100712.

11. Zhao, M., J.J. Whyte, G.M. Hopkins, M.D. Kirk, R.S. Prather. 2014. Methylated DNA immunoprecipitation and high-throughput sequencing (MeDIP-seq) by using low amounts of genomic DNA. Cellular Reprogramming 16:175-184. doi: 10.1089/cell.2014.0002. Epub 2014 Apr 28. PMID: 24773292

E. Provide unique mechanisms for open scientific exchange and dialogue… (IA; MO; NC).

1. Levis, D., Baker, B., Estienne, M., Flowers, W., Knauer, M., Knox, R., Safranski, T., Singleton, and Stalder, K. 2014. National Swine Reproduction Guide. U.S. Pork Center of Excellence. (http://www.usporkcenter.org/Projects/507/NationalSwineReproductionGuide.aspx)

F. Development of innovative teaching methods for undergraduate education… (WI, MO, KS, NC).

1. JJ Parrish, MF Smith, RD Geisert, DL Davis, ME Wilson, WL Flowers. 2015. How to communicate with undergraduate students that lack an Animal Science or agricultural background. Animal Frontiers 5(3):54-59.

06/13/2016

Boar Performance (IL, MS, NC, IN, MO, NE, USMARC)

Daigneault B, McNamara K, Purdy P, Krisher RL, Knox RV, Rodriguez-Zas SL, Miller DJ. 2015. Enhanced fertility prediction of cryopreserved boar sperm using novel sperm function assessment. Andrology. DOI:10.1111/andr.12035.

Gonzalez-Peña, D., R. V. Knox, M. D. MacNeil, and S. L. Rodriguez-Zas. Genetic gain and economic values of selection strategies including semen traits in three- and four-way crossbreeding systems for swine production. 2015. J. Anim. Sci. 93:879-891.

Gonzalez-Pena, D, Knox, R.V., Rodriguez-Zas, S.L. 2016. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study. Theriogenology. 85:335-44.

Knox, R.V., J. M. Ringwelski, K. A. McNamara, M. Aardsma, and M. Bojko. 2015. The effect of extender, method of thawing and duration of storage on in-vitro fertility measures of frozen-thawed boar sperm. Theriogenology 84:407-412.

Knox, R.V. 2015. The fertility of frozen boar sperm when used for artificial insemination. Reprod. Dom. Anim. 50 (Suppl 2):90-7.

Machado S, Kadirvel G, Bovin N, Miller DJ. 2015. Oviduct glycans regulate Ca2+ influx, protein tyrosine phosphorylation and viability of sperm. Submitted.

Miller DJ. 2015. Regulation of sperm function by oviduct fluid and epithelium: Insight into the role of glycans. Reprod Domestic Anim 50(Suppl 2): 31-39. doi: 10.1111/rda.12570.

Pedroso-Silva E, Frost D, Li L, Bovin N, Miller DJ. 2016. Identification of lactadherin as a Lewis X trisaccharide binding protein in porcine sperm. (Submitted)

Feugang JM, Greene JM, Sanchez-Rodríguez HL, Stokes JV, Crenshaw MA, Willard ST, Ryan PL. Profiling of relaxin and its receptor proteins in boar reproductive tissues and spermatozoa. Reprod Biol Endocrinol. 2015 May 20;13:46. doi: 10.1186/s12958-015-0043-y.

Flowers, W.L. 2015. Factors affecting the efficient production of boar sperm. Reprod. Dom. Anim. 50(suppl. 2), 25-30.

Flowers, W.L. 2016. Current strategies in boar management. Proceedings, Reproduction Workshop, 2016 Annual Meeting of the American Association of Swine Veterinarians, 17- 23.

Cabezón FA, Stewart KR, Schinckel AP, Barnes W, Boyd RD, Wilcock P, Woodliff J.  Effect of natural betaine on estimates of semen quality in mature AI boars during summer heat stress. Anim Reprod Sci. 2016 Mar 22. pii: S0378-4320(16)30095-1. doi: 10.1016/j.anireprosci.2016.03.009.

Desaulniers, A.T., W.R. Lamberson, T.J. Safranski. 2016. Prenatal heat stress reduces male anogenital distance at birth and adult testis size, which are rescued by concurrent maternal Artemisia absinthium consumption. J. Thermal Biology, 57: 84-91.

Brauer, V.M., J.R. Wiarda Bell, A.T. Desaulniers, R.A. Cederberg and B.R. White. 2016. Functional activity of the Gnrhr2 gene promoter in testis-derived cells is partially conferred by nuclear factor-B, specificity protein 1 and 3 (SP1/3) and overlapping early growth response 1/SP1/3 binding sites. Gene (In Press).

Desaulniers, A.T., R.A. Cederberg, G.A. Mills, J.J. Ford, C.A. Lents and B.R. White. 2015. LH-independent testosterone secretion is mediated by the interaction between GnRH2 and its receptor within porcine testes. Biol. Reprod. 93:45.

Thorson, J.F., A.T. Desaulniers, C. Lee, B.R. White, J.J. Ford and C.A. Lents. 2015. The role of RFamide-related peptide 3 (RFRP3) in regulation of the neuroendocrine reproductive and growth axes of the boar. Anim. Reprod. Sci. 159:60-65.

Sow and Gilt Performance (IL, IA, NC, IN, MO, USMARC)

Knox, R.V. Artificial insemination in pigs today. Theriogenology. 2016.85: 83-93.

Knox, R.V. 2015. Recent advancements in the hormonal stimulation of ovulation in swine. Vet. Med.: Research and Reports. 6:309-320.

Nteeba, J., Sanz-Fernandez, M.V., Rhoads, R.P., Baumgard, L.H., Ross J.W., Keating, A.F. 2015 Heat stress alters ovarian insulin mediated phosphatidylinositol-3 kinase and steroidogenic signaling in gilt ovaries. Biology of Reproduction; 92:148.

Flowers, W.L. 2016. An update of factors affecting sow longevity. Proceedings, Reproduction Workshop, 2016 Annual Meeting of the American Association of Swine Veterinarians, 32-37.

An R, Wang C, Turek J, Machaty Z, Nolte DD. Biodynamic imaging of live porcine oocytes, zygotes and blastocysts for viability assessment in assisted reproductive technologies. Biomed Opt Express. 2015; 6:963-976.

Lee K, Davis A, Zhang L, Ryu J, Spate LD, Park KW, Samuel MS, Walters EM, Murphy CN, Machaty Z, Prather RS. Pig oocyte activation using a Zn²⁺ chelator, TPEN. Theriogenology. 2015; 84:1024-1032.

Wang C, Zhang L, Jaeger LA, Machaty Z. Store-operated Ca2+ entry sustains the fertilization Ca2+ Signal in pig eggs. Biol Reprod. 2015; 93:25.

Calderón Díaz JA, Vallet JL, Lents CA, Nonneman DJ, Miles JR, Wright EC, Rempel LA, Cushman RA, Freking BA, Rohrer GA, Phillips C, DeDecker A, Foxcroft G, Stalder K. 2015. Age at puberty, ovulation rate, and uterine length of developing gilts fed two lysine and three metabolizable energy concentrations from 100 to 260 d of age. Journal of Animal Science 93(7):3521-3527.

Freking BA, Lents CA, Vallet JL. 2016. Selection for uterine capacity improves lifetime productivity of sows. Animal Reproduction Science 167:16-21.

Miles JR, Vallet JL, Ford JJ, Freking BA, Oliver WT, Rempel LA. 2015. Contributions of the maternal uterine environment and piglet genotype on weaning survivability potential: II. Piglet growth, lactation performance, milk composition, and piglet blood profiles during lactation following reciprocal embryo transfers between Meishan and White crossbred gilts. Journal of Animal Science 93(4):1555-1564.

Rempel LA, Vallet JL, Lents CA, Nonneman DJ. 2015. Measurements of body composition during late gestation and lactation in first and second parity sows and its relationship to piglet production and post-weaning reproductive performance. Livestock Science 178:289-295.

Vallet JL, Calderón-Díaz JA, Stalder KJ, Phillips C, Cushman RA, Miles JR, Rempel LA, Rohrer GA, Lents CA, Freking BA, Nonneman DJ. 2016. Litter-of-origin trait effects on gilt development. Journal of Animal Science 94(1):96-105.

Vallet JL, McNeel AK, Miles JR, Freking BA. 2014. Placental accommodations for transport and metabolism during intra-uterine crowding in pigs. Journal of Animal Science and Biotechnology 5(1):55.

Vallet JL, Miles JR, Rempel LA, Nonneman DJ, Lents CA. 2015. Relationships between day one piglet serum immunoglobulin immunocrit and subsequent growth, puberty attainment, litter size, and lactation performance. Journal of Animal Science 93(6):2722-2729.

Wright EC, Miles JR, Lents CA, Rempel LA. 2016. Uterine and placenta characteristics during early vascular development in the pig from day 22 to 42 of gestation. Animal Reproduction Science 164:14-22.

Growth, Development, Physiology (BARC, IA, NC, MO, USMARC)

Ramsay TG, Stoll MJ, Blomberg le A, Caperna TJ. 2016. Regulation of cytokine gene expression by orosomucoid in neonatal swine adipose tissue. Journal of Animal Science and Biotechnology 7:25. doi: 10.1186/s40104-016-0081-0.

Ramsay TG, Blomberg L, Caperna TJ. 2016. α 1-acid glycoprotein inhibits lipogenesis in neonatal swine adipose tissue. Animal (5):812-820. doi: 10.1017/S1751731115002414.

Johnson, J.S., Sanz-Fernandez, M.V., Patience, J.F., Ross, J.W., Gabler, N.K., Lucy M.C., Safranski, T.J., Rhoads, R.P., Baumgard, L.H. 2015 Effects of in utero heat stress on postnatal body composition in pigs: I. Growing Phase. Journal of Animal Science; 93:71-81.

Johnson, J.S., Sanz-Fernandez, M.V., Gutierrez, N.A., Patience, J.F., Ross, J.W., Gabler, N.K., Lucy M.C., Safranski, T.J., Rhoads, R.P., Baumgard, L.H. 2015 Effects of in utero heat stress on postnatal body composition in pigs: II. Finishing Phase. Journal of Animal Science; 93:82-92.

Sanz Fernandez, M.V., Johnson, J.S., Abuajamieh, M., Stoakes, S.K., Seibert, J.T., Cox, L., Kahl, S., Elsasser, T.H., Ross, J.W., Isom, S.C., Rhoads, R.P., Baumgard, L.H.. 2015. Effects of heat stress on carbohydrate and lipid metabolism in pigs. Physiological Reports; 3: e12315.

Cruzen, S.M., Boddicker, R.L., Graves, K., Johnson, T.P., Arkfeld, E.K., Baumgard, L.H., Ross, J.W., Safranski, T.J., Lucy, M.C., Lonergan, S.M. 2015 Carcass composition of market weight pigs subjected to heat stress in utero and during finishing. Journal of Animal Science; 93:2587-2596.

Waide, E.H., Dekkers, J.C.M., Ross, J.W., Rowland, R.R.R., Wyatt, C.R., Ewen, C.L., Thekkoot, D.M. Boddicker, N.J., Tuggle, C.K. 2015. Not all SCID pigs are created equally: Two independent mutations in the Artemis gene cause Severe Combined Immune Deficiency (SCID) in pigs. Journal of Immunology 195:3171-3179.

Johnson, J.S., M. Abuajamieh, M.V. Sanz Fernandez, J.T. Seibert, S.K. Stoakes, A.F. Keating, J.W. Ross, J.T. Selsby, R.P. Rhoads, and L.H. Baumgard. 2015. The impact of in utero heat stress and nutrient restriction on progeny body composition. Journal of Thermal Biology; 53; 143-150.

Ross J.W., Hale B.J., Gabler N.K., Rhoads R.P., Keating A.F., Baumgard L.H. 2015 Physiological consequences of heat stress in pigs. Animal Production Science 55:1381-1390.

Lima, H., Jacobi, S., Man, C., Walker, K., Sommer, J., Flowers, W., Blikslager, A., Xi, L., and Odle, J. Effects of methylating vitamins and docosahexaenoic acid supplementation on intrauterine growth retardation in a feed-restricted swine model. FASEB Journal 28, 112-121.

Huang, Y.L., Ashwell, M.S., Fry, R.S., Lloyd, K.E., Flowers, W.L. and Spears, J.W. 2015. Effect of dietary copper amount and source on copper metabolism and oxidative stress of weanling pigs. J. Anim. Sci. 93, 2948-2958.

Mao, J., M.-T. Zhao, K.M. Whitworth, L.D. Spate, E.M. Walters, C. O’Gorman, K. Lee, M.S. Samuel, C.N. Murphy, R.S. Prather. 2015. Oxamflatin treatment enhances cloned porcine embryo development and nuclear reprogramming. Cellular Reprogramming 17:28-40. doi: 10.1089/cell.2014.0075. Epub 2014 Dec 30. PMID: 2548976.

Huang, J., H. Zhang, X. Wang, K. Dobbs, J. Yao, G. Qin, K.M. Whitworth, E.M. Walters, R.S. Prather, J. Zhao. 2015. Preimplantation porcine embryo development impairment by histone demethylase KDM5B knockdown and disturbance of bi-valent H3K4me3-H3K27me3 modifications. Biol. Reprod. 92: 72. doi: 10.1095/biolreprod.114.122762. Epub 2015 Jan 21. PMID: 25609834.

Redel, B.R., K. Tessanne, L.D. Spate, C.N. Murphy, R.S. Prather. 2015. Arginine increases development of in vitro produced embryos and affects the PRMT-DDAH-NO axis. Reproduction, Fertility & Development 27:655-666. Mar 13. doi: 10.1071/RD14293. PMID: 25765074.

Spate, L.D., A.N. Brown, B.K. Redel, K.M. Whitworth, R.S. Prather. 2015. PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT. Mol Reprod Dev 82:315-320. doi: 10.1002/mrd.22474. Epub 2015 Mar 16. PMID: 25776657.

Park, J., L. Lai, M. Samuel, D. Wax, R.S. Prather, X. Tian. 2015. Disruption of mitochondrionto-nucleus interaction in deceased cloned piglets. PLoS ONE 10(6);E0129378. doi: 10.1371/journal.pone.0129378. PMID 26067091.

Whitworth, K.M., J. Mao, K. Lee, W.G. Spollen, M.S. Samuel, E.M. Walters, L.D. Spate, R.S. Prather. 2015. Transcriptome analysis of pig in vivo, in vitro fertilized and nuclear transfer blastocyst stage embryos treated with histone deacetylase inhibitors post-fusion and activation reveals changes in the lysosomal pathway. Cellular Reprogramming 17:243-258. Doi 10.1089/cell.2015.0022. PMCID: PMC4529055.

Redel, B.K., L.D. Spate, K. Lee, J. Mao, K.M. Whitworth, R.S. Prather. 2016. Glycine supplementation in vitro enhances porcine preimplantation embryo development. Molecular Reproduction & Development Doi:10.1002/mrd.22618. Epub 2016 Feb 8. 83:246-258. PMID 26824641.

Krisher, R.L., A. Heuberger, M. Paczkowski, J. Stevens, C. Popsil, L.D. Spate, R.S. Prather, R.G. Sturmey, J.R. Herrick, W.B. Schoolcraft. 2015. Applying metabolomics analyses to the practice of embryology: Physiology, development and ART. Reprod. Fertil. Devel. 27:602-620. Mar 13. doi: 10.1071/RD14359. PMID: 25763765.

Calderón Díaz JA, Vallet JL, Prince TJ, Phillips CE, DeDecker AE, Stalder KJ. 2015. Optimal dietary energy and amino acids for gilt development: Growth, body composition, feed intake, and carcass composition traits. Journal of Animal Science 93(3):1187-1199.

Lents CA, Brown-Brandl TM, Rohrer GA, Oliver WT, Freking BA. 2016. Plasma concentrations of acyl-ghrelin are associated with average daily gain and feeding behavior in grow-finish pigs. Domestic Animal Endocrinology 55:107-113.

Wells JE, Berry ED, Kalchayanand N, Rempel LA, Kim M, Oliver WT. 2015. Effect of lysozyme or antibiotics on fecal zoonotic pathogens in nursery pigs. Journal of Applied Microbiology 118(6):1489-1497.

Basic Science and Technology (BARC, IA, USMARC, MS, MO, NE)

Caperna TJ, Shannon AE, Stoll M, Blomberg LA, Ramsay TG. 2015. Regulation of alpha-1 acid glycoprotein synthesis by porcine hepatocytes in monolayer culture. Domestic Animal Endocrinology 52:51-59. doi: 10.1016/j.domaniend.2015.02.002.

Zhao,J., W. Xu, W., Ross, J. W., Walters, E.M., Butler, S.P., Whyte, J.J., Kelso, L., Fatemi, M., Vanderslice, N.C., Giroux, K., Spate, L.D., Samuel, M.S., Murphy, C.N., Wells, K., Prather, R.S., Masiello, N., Velander, W.H. 2015 Tissue engineering of the mammary gland to produce abundant hemophilia B therapy in milk. Scientific Reports 5:14176.

Selsby J.T., Ross J.W., Nonneman, D., Hollinger, K. 2015 Porcine models of muscular dystrophy. ILAR Journal; 56:116-126.

Vasquez ES, Feugang JM, Willard ST, Ryan PL, Walters KB. Bioluminescent magnetic nanoparticles as potential imaging agents for mammalian spermatozoa. J Nanobiotechnology. 2016 Mar 17;14:20. doi: 10.1186/s12951-016-0168-y.

Feugang JM, Youngblood RC, Greene JM, Willard ST, Ryan PL. Self-illuminating quantum dots for non-invasive bioluminescence imaging of mammalian gametes. J Nanobiotechnology. 2015 Jun 4;13:38. doi: 10.1186/s12951-015-0097-1.

Schook, L.B., T.V. Collares, W. Hu, Y. Lian, F.M. Rodrigues, L.A. Rund, K.M. Schachtschneider, F. K. Seixas, K. Singh, K.D. Wells, E.M. Walters, R.S. Prather, C.M. Counter. 2015. A genetic porcine model of cancer. PLoS ONE doi:10.1371/journal.pone.0128864. PMID: 26132737.

Men, H., L.D. Spate, C.N. Murphy, R.S. Prather 2015. Cryopreservation of in vitro-produced early stage porcine embryos in sealed straws. BioResearch Open Access 4:258-265. Doi: 10.1089/biores.2015.0012. PMID: 26309801.

Beaton, B.P., Y.-J. Choi, D.-N. Kim, J.-H. Kim, M.S. Samuel, J.A. Benne, K.D. Wells, K. Lee, J.-H. Kim, R.S. Prather. 2015. Inclusion of homologous DNA in nuclease-mediated gene targeting facilitates a higher incidence of bi-allelically modified cells. Xenotransplantation (18 SEP 2015 | DOI: 10.1111/xen.12194). 22:379-390.

Redel, B.K., R.S. Prather. 2015. Meganucleases revolutionize the production of genetically engineered pigs for the study of human diseases. Special Swine issue (T. Monticello & W. Haschek-Hock, eds) of Toxicologic Pathology (DOI | 10.1177/0192623315613160) 1-6.

Cederberg, R.A., J.E. Smith, E.A. McDonald, C. Lee, A.R. Perkins and B.R. White. 2015. Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region, two proximal GSEs and a retinoid X receptor binding site. Reprod. Biol. Endocrinol. 13:45.

Genetics and Genomics (MO, USMARC)

Whitworth, K.M, R.R.R. Rowland, C.L. Ewen, B.R. Trible, M.A. Kerrigan, A.G. Cino-Ozuna, M.S. Samuel, J.E. Lightner, D.G. McLaren, A. Mileham, K.D. Wells, R.S. Prather. 2016. Gene edited pigs are protected from porcine reproductive and respiratory syndrome virus. Nature Biotechnology (7 DEC 2015 | DOI: 10.1038/nbt.3434). 34:20-22. PMID 26641533.

Bernal Rubio YL, Gualdrón Duarte JL, Bates RO, Ernst CW, Nonneman D, Rohrer GA, King A, Shackelford SD, Wheeler TL, Cantet RJC, Steibel JP. 2016. Meta-analysis of genome-wide association from genomic prediction models. Animal Genetics 47(1):36-48.

Cagnone G, Tsai T-S, Srirattana K, Rossello F, Powell DR, Rohrer G, Cree L, Trounce IA, St.John JC. 2016. Segregation of naturally occurring mitochondrial DNA variants in a mini-pig model. Genetics 202(3):931-944.

Nonneman DJ, Schneider JF, Lents CA, Wiedmann RT, Vallet JL, Rohrer GA. 2016. Genome-wide association and identification of candidate genes for age at puberty in swine. BMC Genetics 17(1):50.

Rohrer GA, Brown-Brandl TM, Nonneman DJ. 2014. Characterization of a novel porcine stress syndrome. National Swine Improvement Federation. NSIF Volume 36.

Rohrer GA, Nonneman DJ, Wiedmann RT, Schneider JF. 2015. A study of vertebra number in pigs confirms the association of vertnin and reveals additional QTL. BMC Genetics 16(1):129.

Schneider JF, Miles JR, Brown-Brandl TM, Nienaber JA, Rohrer GA, Vallet JL. 2015. Genomewide association analysis for average birth interval and stillbirth in swine. Journal of Animal Science 93(2):529-540.

Wiedmann RT, Nonneman DJ, Rohrer GA. 2015. Genome-wide copy number variations using SNP genotyping in a mixed breed swine population. PLoS One 10(7):e0133529.

Teaching and Extension (WI, MO, KS, WV, NC)

Parrish, J.J., Smith, M.F., Geisert, R.D., Davis, D.L., Wilson, M.E. and Flowers, W.L. 2015. How to communicate with undergraduate students that lack an animal science or agricultural background. Animal Frontiers 5, 54-59.

08/16/2017

Boar Performance (IN, NE, IL)

A. Proctor, D. W. Lugar, M. C. Lucy, T. J. Safranski and K. R. Stewart. 2017. Effects of in Utero Heat Stress on Boar Growth and Reproduction Prior to, during, and after Puberty. Midwest ASAS, Omaha, NE. Abstract.

W. Lugar, W. A. Krom, J. A. Proctor, P. D. Mings, and K. R.Stewart. 2017. Effects of Supplemental Betaine to Semen Extenders on Semen Quality in Boars. Midwest ASAS, Omaha, NE. Abstract.

Lents, C.L., J.F. Thorson, A.T. Desaulniers and B.R. White.  2017.  RFamide-related peptide 3 and gonadotropin-releasing hormone-II are autocrine-paracrine regulators of testicular function in the boar.  Mol. Reprod. Dev.  May 5.  doi: 10.1002/mrd.22830.  [Epub ahead of print] Review.

Gonzalez-Pena D, Knox RV, Rodriguez-Zas SL. 2016. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study. Theriogenology. 85:335-44.

Silva E, Frost D, Li L, Bovin N, Miller DJ. 2017. Lactadherin is a candidate oviduct Lewis X trisaccharide receptor on porcine sperm. Andrology doi: 10.1111/andr.12340

Winters RA, Nettenstrom LM, Lopez DG, Willenburg KL, Vishwanath R, Miller DJ. 2017. Effect of sorting porcine spermatozoa by sex chromosomes on oviduct cell binding. Submitted.

Sow and Gilt Performance (IN, NE, IL, KS, USMARC)

 Cabezon, F. K.R. Stewart, A.P. Schinckel, B.T. Richert, M. Gandarillas, M. Pasache, and W.A. Peralta.  2016.  Effect of betaine supplementation during summer on sow lactation and subsequent farrowing performance. Professional Animal Scientist, 32(5), pp 698-706.

Cabezón, F.A., Schinckel, A.P., Richert, B.T., Stewart, K.R., Gandarillas, M. and Peralta, W.A., 2016. Analysis of lactation feed intakes for sows including data on environmental temperatures and humidity. The Professional Animal Scientist, 32(3), pp.333-345.

N.M. Chapel, C.J. Byrd, D. W. Lugar, K. R. Stewart, T.J. Safranski, L.H. Baumgard, and J.S. Johnson.  2017.  The effects of in utero heat stress on fasting heat production in growing pigs.  Midwest ASAS, Omaha, NE. Abstract.

Thorson, J.F., N.L. Heidorn, V. Ryu, K. Czaja, D.J. Nonneman, C.R. Barb, G.J. Hausman, G.A. Rohrer, L.D. Prezotto, R.B. McCosh, E.C. Wright, B.R. White, B.A. Freking, W.T. Oliver, S.M. Hileman and C.A. Lents.  2017.  Neuropeptide FF receptor function affects gonadotropin secretion and age at puberty in gilts.  Biol. Reprod.  96:617-634.

Knox RV, Esparza-Harris KC, Johnston ME, Webel SK. 2017. Effect of numbers of sperm and timing of a single, post-cervical insemination on the fertility of weaned sows treated with OvuGel®. Theriogenology 92:197-203.

Knox RV, Shen J, Greiner LL, Connor JF. 2016. Effect of timing of relocation of replacement gilts from group pens to individual stalls before breeding on fertility and well-being. J. Anim. Sci. 94:5114-5121.

Knox RV. Artificial insemination in pigs today. Theriogenology. 2016.85: 83-93.

Arend L, Knox RV, Greiner L, Graham A, Connor J.  2016.  Effects of feeding melatonin during proestrus and early gestation in gilts and P1 sows to minimize the effects of seasonal infertility. Midwest ASAS/ADSA Annual Meeting.

Arend LS, Duangkamol P, Knox RV. 2016. Administration of melatonin during the follicular and early luteal phase to mimic short days and minimize seasonal infertility for prepubertal gilts housed under differing hours of light and heat. Allen D. Leman Swine Conf. St. Paul, MN.

Vaughn, M., C. Serrano, D. Burnett, D. Davis, J. Woodworth, and J. Gonzalez.  2016. Influence of Porcine Plasma Supplementation on Gestating Sow Serum IGF-1 Concentration and Litter Weights. Professional Agricultural Workers Journal. 4: No. 1,

L. Frobose, M. D. Tokach, J. M. DeRouchey, S. S. Dritz, R. D. Goodband, J. L. Nelssen, and D. L. Davis. 2017. Follicle development, incidence of lactational estrus, and ovulation in sows exposed to different suckling reduction strategies. International Conference on Pig Reproduction. June 11-14, Columbia, MO.

J.M. Morton, A.J. Langemeier, T. Rathbun, D.L. 2017. Split suckling and birth weight affects colostrum intake and pre-weaning weight gain. International Conference on Pig Reproduction. June 11-14, Columbia, MO.

Vallet JL, Meyer S. 2016. Effect of glucosamine supplementation on litter size in a commercial setting - NPB #14-238. National Pork Board.

Vallet, J.L., Miles, J.R.  2017. The effect of farrowing induction on colostrum and piglet serum immunocrits is dependent on parity.  Journal of Animal Science 96(2): 688-696.  doi:10.2527/jas.2016.0993.

Growth, Development, Physiology (NE, IL, USMARC)

Riccio G, Ellerbrock R, Canisso I, Knox R, Kline K. 2016. Motility of stallion spermatozoa after centrifugation and cooling in INRA96® or Walworth Extender. Journal of Agricultural Science and Technology. A 6 143-147.

Silva E, Miller DJ. 2016. Involvement of specific oviduct cell glycans in sperm binding to form a reservoir. J Vet Andrology 1:24-28.

Phoophitphong D., Tummaruk P, Knox RV. 2016. Serum estradiol-17B concentration after estrus induction using PG600 in gilts. 24^th International Pig Veterinary Society Congress. P0-PW1-247.

Tummaruk T, Phoophitphong D, Knox RV. Follicle development, ovulation, and evidence of silent heat in gilts after estrus induction using gonadotrophin under tropical climate. 24^th International Pig Veterinary Society. Dublin. 2016. PO-PW1-239.

Lents CA, Brown-Brandl TM, Rohrer GA, Oliver WT, Freking BA. 2016. Plasma concentrations of acyl-ghrelin are associated with average daily gain and feeding behavior in grow-finish pigs. Domestic Animal Endocrinology. 55:107-113.

Rempel LA, Miles JR, Parrish J. 2016. Evaluation of contributions to seasonal reproductive inefficiency; NPB #14-052. National Pork Board. Available: http://research.pork.org/FileLibrary/ResearchDocuments/14-052-REMPEL-final%20rpt.pdf.

Thorson, J., Heidorn, N.L., Ryu, V., Czaja, K., Nonneman, D.J., Barb, C.R., Hausman, G.J., Rohrer, G.A., Prezotto, L.D., McCosh, R.B., Wright, E.C., White, B.R., Freking, B.A., Oliver, W.T., Hileman, S.M., Lents, C.A. 2017.  Relationship of neuropeptide FF receptors with pubertal maturation of gilts. Biological Reproduction 96(3):617-634.  doi:10.1095/biolreprod.116.144998.

Basic Science and Technology (IN, KS, NE, IL)

Herrick JR, Wang C, Machaty Z. 2016. The effects of permeating cryoprotectants on intracellular free-calcium concentrations and developmental potential of in vitro-matured feline oocytes. Reprod Fertil Dev 28:599-607.

Machaty Z. 2016. Signal transduction in mammalian oocytes during fertilization. Cell Tissue Res 363:169-183.

Silva PV, Zhang L, Jaeger LA, Machaty Z. 2016. Effects of ORAI1 down-regulation on oocyte maturation in pigs. Proc 18^th International Congress on Animal Reproduction. Tours, France, p.280.

Zhang L, Chao C-H, Jaeger LA, Machaty Z. 2016. Repetitive interactions between STIM1 and ORAI1 at fertilization in pig oocytes. Proc 18^th International Congress on Animal Reproduction. Tours, France, p. 50.

Machaty Z, Miller AR, Zhang L. 2017. Egg activation at fertilization. In: “Vertebrate development”. (Eds.: F. Pelegri, M. Danilchik, A. Sutherland). Adv. Exp. Med. Biol., Vol. 953. pp. 1-47. Springer, Cham, Switzerland.

Zhang L, Machaty Z. 2017. Targeted gene knockdown in early embryos using siRNA. In: “Zygotic genome activation”. (Ed.: K. Lee). Methods Mol Biol., pp. 207-217. Humana Press, New York, NY, USA.

Morton, T. Rathbun and D.L. Davis. 2016. Relationships between pig weights at birth and weaning and expression of EGR1, Pref1, Cox1 and Cox2 in Wharton’s jelly and epididymal fat pad. Proc. Perinatal Biology Symposium: Interconnecting Animal and Human Systems to Understand Life-Disease.

Brauer, V.M., J.R. Wiarda Bell, A.T. Desaulniers, R.A. Cederberg and B.R. White.  2016.  Functional activity of the Gnrhr2 gene promoter in testis-derived cells is partially conferred by nuclear factor-kB, specificity protein 1 and 3 (SP1/3) and overlapping early growth response 1/SP1/3 binding sites.  Gene 587:137-146.

Xie, F., R.L. Krisher and J.R. Wood.  2016.  Oxidative stress during oocyte in vitro maturation increases the abundance of Dppa3 and Pou5f1 maternal effect gene transcripts in matured oocytes and two-cell embryos, indicative of altered post-transcriptional regulation of maternal mRNAs.  Society for the Study of Reproduction 48th Annual Meeting, San Diego, CA. p. 344.

Daigneault B, Miller DJ.  2017. Transient receptor potential cation channel subfamily P member 2 (TRPP2) regulates motility and intracellular calcium of porcine sperm. Submitted.

Sharif M, Silva E, Shah ST, Miller DJ. 2017. Redistribution of soluble N-ethylmaleimide-sensitive-factor attachment protein receptors in mouse sperm membranes prior to the acrosome reaction. Biol Reprod 96:352-365. doi: 10.1095/biolreprod.116.143735, PMID: 28203732

Daigneault BW and DJ Miller. 2016. TRPP2 regulates motility and intracellular calcium of porcine sperm. 42^nd IETS Annual Conference.

Dutta S and DJ Miller. 2016. Oviduct glycans prolong the lifespan of bovine sperm. 49^th Annual Meeting of the Society for the Study of Reproduction.

Elsokary MM and DJ Miller.  2017.  Mature oocytes trigger the release of bovine sperm from an immobilized oviduct glycan.  43^rd IETS Annual Conference. Austin, TX  Reprod Fertil Dev

Elsokary MM, Miller DJ.  2017.  Mature cumulus-oocyte complexes trigger the release of bovine sperm from an immobilized oviduct glycan.  50^th Annual Meeting of the Society for the Study of Reproduction.

Lotti S, Rubessa M, Knox RV, Wheeler MB. 2017. Effects of liposomes on sperm motility and DNA binding efficiency. International Embryo Transfer Society.

Machado SA, Miller DJ. 2017. The release of porcine sperm from oviduct cells is induced by progesterone and requires CatSper. 50^th Annual Meeting of the Society for the Study of Reproduction.

Machado SA, Sharif M, Kadirvel G, Bovin N, Miller DJ. 2017. Oviduct glycans regulate sperm Ca²⁺ influx and sperm viability. 50^th Annual Meeting of the Society for the Study of Reproduction.

Sharif M, Kerns K, Sutovsky P, Miller DJ.  2017.  Proteasomal activity is necessary for porcine sperm release from immobilized oviduct glycans. 50^th Annual Meeting of the Society for the Study of Reproduction.

Winters R and Miller DJ. 2016. Effect of sorting boar spermatozoa by sex chromosomes on oviduct cell binding.  49^th Annual Meeting of the Society for the Study of Reproduction.

Winters RA, Hamilton DN, Bhatnagar AS, Fitzgerald R, Bovin N, Miller DJ. 2017. Porcine sperm binding to oviduct cells and their glycans as supplements to laboratory semen analysis. 50^th Annual Meeting of the Society for the Study of Reproduction.

Genetics and Genomics (NE, USMARC)

Desaulniers, A.T., R.A. Cederberg, G.A. Mills, C.A. Lents and B.R. White.  2017.  Production of a gonadotropin-releasing hormone 2 receptor knockdown (GnRHR2 KD) swine line.  Transgenic Res.  May 22.  doi: 10.1007/s11248-017-0023-4.  [Epub ahead of print].

McDonald, E.A., J.E. Smith, R. A. Cederberg and B.R. White.  2016.  Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites.  Reprod. Biol. Endocrinol. 14:36.

Cagnone G, Tsai T-S, Srirattana K, Rossello F, Powell DR, Rohrer G, Cree L, Trounce IA, St John JC. 2016. Segregation of naturally occurring mitochondrial DNA variants in a mini-pig model. Genetics. 202(3):931-944.

Desaulniers AT, Cederberg RA, Lents CA, White BR. 2016. Use of genetically engineered swine to elucidate testis function in the boar. Large Animal Genetic Engineering Summit. September 18-20 2016, Bethesda, Maryland, p. 15-16, Symposium Proceedings. (2pp).

Freking BA, Lents CA, Vallet JL. 2016. Selection for uterine capacity improves lifetime productivity of sows. Animal Reproduction Science. 167:16-21.

Nonneman, D.J., Lents, C.A., Keel, B.N., Rohrer, G.A.  2017.  USMARC update on swine reproduction research.  In proceedings: Plant and Animal Genome Conference XXIV. Jan. 14-18, 2017. San Diego, CA. W914.

Rohrer, G.A., Cross, A.J., Lents, C.A., Miles, J.R., Nonneman, D.J., Rempel, L.A. 2017.  Genetic improvement of sow lifetime productivity. [abstract]  Journal of Animal Science 95(Suppl 2), p.11-12.

Rohrer, G.A., Nonneman, D.J.  2017.  Genetic analysis of teat number in pigs reveals some developmental pathways independent of vertebra number and several loci which only affect a specific side.  Genetics Selection Evolution 49:4.  doi:10.1186/s12711-016-0282-1.

White, B.R., Desaulniers, A.T., Cederberg, R.A., Mills, G.A., Lents, C.A. 2017.  A transgenic boar model to elucidate the role of gonadotropin-releasing hormone 2 (GnRH2) and its receptor in regulating testes and sperm function.  Journal of Animal Science 95(Suppl 2) p. 150.  doi:10.2527/asasmw.2017.308.

Wijesena, H.R., Lents, C.A., Reithoven, J., Trenhaile-Grannemann, M.D., Thorson, J.F., Keel, B.N., Miller, P.S., Spangler, M.L., Kachman, S.D., Ciobanu, D.C.  2017.  Integration of genomic approaches to uncover sources of variation in age at puberty and reproductive longevity in sows.  Journal of Animal Science 95 (Suppl 2) p.12.  doi:10.252

Teaching and Extension (NE, IL)

Desaulniers, A.T., R.M. McFee and B.R. White.  2016.  Smoothing the transition from classroom to hog farm:  Use of case studies to enhance swine industry knowledge and interest in college students.  Nebraska Pork Talk, Nebraska Pork Producers Association. 48:14-15.

Desaulniers, A.T., R.M. McFee and B.R. White.  2016.  Case-based reasoning enhances learning in an undergraduate reproductive biology course.  Society for the Study of Reproduction 48th Annual Meeting, San Diego, CA. p. 199.

Gonzalez-Pena D, Knox RV, Rodriguez-Zas SL. 2016. Contribution of semen trait selection, artificial insemination technique, and semen dose to the profitability of pig production systems: A simulation study. Theriogenology. 85:335-44.

 

06/15/2018

08/29/2019

Publications (citations in bold are from more than one station):

Boar Performance (IL, IN, NE, USMARC, WI)

Gruhot, T.R., L.A. Rempel, M.L. Spangler, S.D. Kachman, and B.E. Mote. 2019. The heritability of pampiniform plexus vessel size and varicocele in boars. Reprod. Dom. Anim. 54:270-274.

Hufana-Duran D., P.G. Duran, R. Monson, J.J. Parrish. 2017. Motility and membrane integrity of ejaculated bovine spermatozoa extended and cryopreserved in L-Carnitine Tris-Egg yolk extender. J. International Society for Southeast Asian Agriculture Sciences 23:56-67.

Lugar, D.W., J.A. Proctor, T.J. Safranski, M.C. Lucy, and K.R. Stewart. 2018. In utero heat stress causes reduced testicular area at puberty, reduced total sperm production, and increased sperm abnormalities in boars. Anim. Repro. Sci. 192:126-135.

Rempel L.A., M.M. Krautkramer, T.M. Loether, J.J. Parrish, and J.R. Miles. 2018. Season of collection and sperm head shape impacts expression of CARHSP and FTL from motile-rich boar sperm. Agri Gene 7:1-6.

Rempel, L.A., M.M. Krautkramer, J.J. Parrish, and J.R. Miles. 2019. Impact of seasonality, storage of semen, and sperm head-shape on whole tissue methylation and expression of methylation responsive candidate genes in swine placenta and fetal livers from summer and winter breedings. Mol. Reprod. Dev. 86:465-475.

Winters R.A., D. Hamilton, A. Bhatnagar, R. Fitzgerald, N. Bovin, and D.J. Miller. 2018. Porcine sperm binding to oviduct cells and glycans as supplements to traditional laboratory semen analysis. J. Anim. Sci. 96:5265-5275.

Winters R.A., L.M. Nettenstrom, D.G. Lopez, K.L. Willenburg, R. Vishwanath, and D.J. Miller. 2018. Effect of sorting porcine spermatozoa by sex chromosomes on oviduct cell binding. Theriogenology 108:22-28.

Sow and Gilt Performance (IA, IL, IN, USMARC)

Chapel, N.M., J.S. Radcliffe, K.R. Stewart, J.R. Lucas, and D.C. Lay Jr. 2018. The impact of farrowing room noise on sows’ reactivity to piglets.  Translational Anim. Sci. 3:175-184.

Dickson, M., C.L. Hager, A. Al-Shaibi, P. Thomas, L.H. Baumgard, J.W. Ross, and A.F. Keating, 2018 Impact of heat stress during the follicular phase on porcine ovarian steroidogenic and phosphatidylinositol-3 signaling. J. Anim. Sci. 96:2162-2174.

George, A.F., T. Ho, N. Prasad, B.N. Keel, J.R. Miles, J.L. Vallet, F.F. Bartol, and C.A. Bagnell. 2019. Neonatal lactocrine deficiency affects the adult porcine endometrial transcriptome at pregnancy day 13. Biol. Reprod. 100:71-85.

Graves, K.L., J.T. Seibert, A.F. Keating, L.H. Baumgard, and J.W. Ross. 2018. Characterizing the acute heat stress response in gilts: II. Assessing repeatability and association with fertility. J. Anim. Sci. 96:2419-2426.

Knox, R.V. Physiology and Endocrinology Symposium: Factors influencing follicle development in gilts and sows and management strategies used to regulate growth for control of estrus and ovulation. J. Anim. Sci. 97:1433-45.

Knox, R.V., K.R., Stewart, W.L. Flowers, M.E. Swanson, S.K. Webel, and R.R. Kraeling. 2018. Design and biological effects of a vaginally administered gel containing the GnRH agonist, triptorelin, for synchronizing ovulation in swine. Theriogenology 112:44-52.

Rempel L.A., M.M. Krautkramer, J.J. Parrish, and J.R. Miles. 2019. Impact of seasonality, storage of semen, and sperm head‐shape on whole tissue methylation and expression of methylation responsive candidate genes in swine placenta and fetal livers from summer and winter breedings. Mol. Reprod. Dev. 86:465-475.

Seibert, J.T., K.L. Graves, B.J. Hale, A.F. Keating, L.H. Baumgard, and J.W. Ross, 2018.  Characterizing the acute heat stress response in gilts: I. Thermoregulatory and production variables.  J. Anim. Sci. 96:941-949.

Ulguim R.R., F.P. Bortolozzo, I. Wentz, M. Johnston, S.K. Webel, L. Arend, and R.V. Knox. 2018. Ovulation and fertility responses for sows receiving once daily boar exposure after weaning and OvuGel® followed by a single fixed time post cervical artificial insemination. Theriogenology. 105: 27-33.

Growth, Development, Physiology (IA, IL, MO, USMARC, WI)

Bidne, K.L., M.J. Dickson, J.W. Ross, L.H. Baumgard, and A.F. Keating, 2018. Disruption of reproductive function by gut permeability to endotoxins.   Reproduction 155:R169-R181.

Chen, P.R., B.K. Redel, L.D. Spate, T. Ji, S.R. Salazar, and R.S. Prather. 2018. Glutamine supplementation enhances development of in vitro-produced porcine embryos and increases leucine consumption from the culture medium. Biol. Reprod. 99:938-948.

Kerns, K., M. Zigo, E.Z. Drobnis, M. Sutovsky, and P. Sutovsky. 2018. Zinc ion flux during mammalian sperm capacitation. Nat. Commun. 9:2061.

Kerns, K., M. Zigo, and P. Sutovsky. 2018. Zinc: A necessary ion for mammalian sperm fertilization competency. Int. J. Mol Sci. 19(12).

Knox, R.V., D.J. Miller. 2018. Male ART in Animal Species. In:  M. K. Skinner (Ed.), Encyclopedia of Reproduction (2^nd Ed.). Vol. 1, pp. 547–552. Academic Press: Elsevier.

Mayorga E.J., S.K. Kvidera, E.A. Horst, M. Al-Qaisi, M.J. Dickson, J.T. Seibert, S. Lei, A.F. Keating, J.W. Ross, R.P. Rhoads, Z.J. Rambo, M.E. Wilson, and L.H. Baumgard. Effects of zinc amino acid complex on biomarkers of gut integrity and metabolism during and following heat stress or feed restriction in pigs.  J. Anim. Sci. 96:4173-4185.

Meyer, A.E., C.A Pfeiffer, K.E. Brooks, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, C.N. Murphy, S. Behura, M.K. McLean, L.A. Ciernia, M.F. Smith, T.E. Spencer, R.S. Prather, and R.D. Geisert. 2019. New perspective on the role of conceptus estrogens in conceptus development, maternal recognition and establishment of pregnancy in the pig. Biol. Reprod. 101:148-161.

Miller D.J.  2018.  The epic journey of sperm through the female reproductive tract.  Animal 12(Suppl. s1):110-120.

Mordhorst, B.R., J.A. Benne, R.F. Cecil, K.M. Whitworth, M.S. Samuel, L.D. Spate, C.N. Murphy, K.D. Wells, J.A. Green, and R.S. Prather. 2019. Improvement of in vitro and early in utero porcine clone development after somatic donor cells are cultured under hypoxia. Mol. Reprod. Dev. 86:558-565.

Mordhorst, B.R., S.L. Murphy, M. Schaufliner, S. Rojas Salazar, T. Ji, S.K. Behura, K.D. Wells, J.A. Green, and R.S. Prather. 2018. Porcine fetal-derived fibroblast cells alter gene expression and mitochondria to compensate for hypoxic stress during culture. Cell. Reprogram. 20:225-235.

Mordhorst, B.R., S.L. Murphy, R.M. Ross, J. A. Benne, M.S. Samuel, R.A. Cecil, B.K. Redel, L.D. Spate, C.N. Murphy, K.D. Wells, J. A. Green, and R. S. Prather. 2018. Pharmacologic treatment of donor cells induced to have a Warburg effect-like metabolism does not alter embryonic development in vitro or survival during early gestation when used in somatic cell nuclear transfer in pigs. Mol. Reprod. Dev. 85:290-302.

Mordhorst, B.R., S.L. Murphy, R.M. Ross, M.S. Samuel, T. Ji, S. Rojas Salazar, S.K. Behura, K.D. Wells, J.A. Green, and R.S. Prather. 2018. Pharmacologic reprogramming designed to induce a Warburg Effect in porcine fetal fibroblasts alters gene expression and quantities of spent media metabolites without increased cell proliferation. Cell. Reprogram. 20:38-48.

Parrish, J.J. 2019. Spermatogenesis, heat stress and male infertility, In: Molecular Mechanisms In Spermatogenesis And Male Infertility. Ed: Rajender Singh. CRC Press, Boca Raton, FL, USA. (in press)

Seelenbinder K.M., L.D. Zhao, M.D. Hanigan, M.W. Hulver, R.P. McMillan, L.H. Baumgard, J.T. Selsby, J.W. Ross, N.K. Gabler, and R.P. Rhoads, 2018.  Effects of heat stress during porcine reproductive and respiratory syndrome virus infection on metabolic responses in growing pigs. J. Anim. Sci. 96:1375-1387.

Seibert J.T., M. Abuajamieh, M.V. Sanz Fernandez, J.S.Johnson, S.K.Kvidera, E.A. Horst, E.J. Mayorga, S. Lei, J.F. Patience, J.W. Ross, R.P. Rhoads, R.C. Johnson, S.M. Lonergan, J.W. Perfield, 2nd, and L.H Baumgard.  2018.  Effects of heat stress and insulin sensitizers on pig adipose tissue. J. Anim. Sci. 96:510-520.

Smith M.F., R.D. Geisert, and J.J. Parrish. 2018. Reproduction in domestic ruminants during the past 50 years: discovery to application. J. Anim. Sci. 96:2952-2970

Sutovsky, P. 2018. Pig overview (male reproduction) In:  M. K. Skinner (Ed.), Encyclopedia of Reproduction (2^nd Ed.). Vol. 1, pp. 501–507. Academic Press: Elsevier.

Whyte, J.J., A.E. Meyer, L.D. Spate, J.A. Benne, R. Cecil, M.S. Samuel, C.N. Murphy, R.S. Prather, and R.D. Geisert. 2018. Inactivation of interleukin-1B (IL1B2) results in failure of rapid conceptus elongation. Proc. Natl. Acad. Sci. U.S.A. 115:307-312.

Zhao, L., R. McMillan, G. Xie, S. Giridhar, L.H. Baumgard, S. El-Kadi, J.T. Selsby, J.W. Ross, N.K. Gabler, M. Hulver, and R. Rhoads. 2018.  Heat stress decreases metabolic flexibility in skeletal muscle of growing pigs. Am J Physiol Regul Integr Comp Physiol. 315:R1096-R1106.

Basic Science and Technology (IA, IL, MO, NE, USMARC)

Abuajamieh, M., S.K. Kvidera, E.J. Mayorga, A. Kaiser, S.M. Lei, J.T. Seibert, E.A. Horst, M.V. Sanz Fernandez, J.W. Ross, J.T. Selsby, A.F. Keating, R.P. Rhoads, and L.H.  Baumgard. 2018 The effect of recovery from heat stress on circulating bioenergetics and inflammatory biomarkers.  J. Anim. Sci. 96:4599-4610.

Bidne K.L., S.S. Kvidera, J.W. Ross, L.H. Baumgard, and A.F. Keating. 2018. Impact of repeated lipopolysaccharide administration on ovarian signaling during the follicular phase of the estrous cycle in post-pubertal pigs.  J. Anim. Sci. 96:3622-3634.

Li, Y., M.K. Adur, B.J. Hale, and J.W. Ross. 2018.  Regulation of oogenesis by noncoding RNAs.  In:  M. K. Skinner (Ed.), Encyclopedia of Reproduction (2^nd Ed.). Vol. 3, pp. 190–196. Academic Press: Elsevier.

Manca, S., B. Upadhyaya, E. Mutai, A.T. Desaulniers, R.A. Cederberg, B.R. White and J. Zempleni. 2018. Milk exosomes are bioavailable and distinct microRNA cargos have unique tissue distribution patterns. Sci. Rep. 8:11321.

Mao, J., C. O’Gorman, M. Sutovsky, M. Zigo, K.D. Wells, and P. Sutovsky. 2018. Ubiquitin A-52 residue ribosomal protein fusion product 1 (Uba52) is essential for preimplantation embryo development. Biol. Open 7(10).

Powell, E.J., S. Charley, A. Boettcher, L. Varley, J. Brown, M. Schroyen, M.K. Adur, S. Dekkers, D. Isaacson, M. Sauer, J. Cunnick, N.M. Ellinwood, J.W. Ross, J.C.M. Dekkers, and C.K.  Tuggle. 2018.  Creating effective biocontainment facilities and maintenance protocols for raising specific pathogen free, severe combined immunodeficient (SCID) swine.  Lab Anim. 52:402-412.

Redel, B.K., B.P. Beaton, J.A. Benne, S.L. Murphy, C.W. O’Gorman, A.M. Spate, R.S. Prather, and K.D. Wells. 2018. Single step production of Cas9 mRNA for zygote injection. BioTechniques, 64:118-124.

Ryu J., R.S. Prather, and K. Lee. 2018. Use of gene-editing technology to introduce targeted modifications in pigs. J. Anim. Sci. Biotechnol. 9:5.

Sutovsky, P., and W. Song. 2018. Post-fertilization sperm mitophagy: The tale of mitochondrial Eve and Steve. Reprod. Fert. Dev. 30:56-63.

Thorson, J.F., L.D. Prezotto, H. Adams, S.L. Petersen, J.A. Clapper, E.C. Wright, W.T. Oliver, B.A. Freking, A.P. Foote, E.D. Berry, D.J. Nonneman, and C.A. Lents. 2018. Energy balance affects pulsatile secretion of luteinizing hormone from the adenohypophesis and expression of neurokinin B in the hypothalamus of ovariectomized gilts. Biol. Reprod. 99:433-445.

Whitworth, K.M., R. Cecil, J.A. Benne, B.K. Redel, L.D. Spate, M.S. Samuel, K.D. Wells, and R.S. Prather. 2018. Zygote injection of RNA encoding Cre recombinase results in efficient removal of LoxP flanked neomycin cassettes in pigs. Transgenic Res. 27:167-178.

Whitworth, K.M., R.R.R. Rowland, V. Petrovan, M. Sheahan, A.G. Cino-Ozuna, J. Nietfeld, Y, Fang, R. Hesse, A. Mileham, M.S. Samuel, K.D. Wells, and R.S. Prather. 2019. Resistance to coronavirus infection in amino peptidase N-deficient pigs. Transgenic Res. 28:21-32.

Zigo, M., K. Kerns, M. Sutovsky, and P. Sutovsky. 2018. Modifications of the 26S proteasome during boar sperm capacitation. Cell Tissue Res. 372:591-601.

Genetics and Genomics (IA, IL, USMARC)

Beiki, H., H. Liu, N. Manchanda, D.J. Nonneman, T.P.L. Smith, J. Reecy, and C. Tuggle. 2019. Improved annotation of the domestic pig genome through integration of Iso-Seq and RNA-seq data. BMC Genomics. 20:344-362.

Fu YR, R. Knox, L. Li, and S. Ren. 2018. Differential gene expression of Eph-ephrin A1 and LEPR-LEP with high or low number of embryos in pigs during implantation. Reprod. Dom. Anim.

Keel, B.N., D.J. Nonneman, A.K. Lindholm-Perry, W.T. Oliver, and G.A. Rohrer. 2018. Porcine single nucleotide polymorphisms and their functional effect: an update. BMC Research Notes 11:860.

Kim, K.S., J.T. Seibert, Z. Edea, K.L. Graves, E.-S. Kim, A.F. Keating, L.H. Baumgard, J.W. Ross, and M.F. Rothschild. 2018. Characterizing the acute heat stress response in gilts: III. Identification of underlying genomic control. J. Anim. Sci. 96:2074-2085.

Teaching and Extension (NE)

McFee, R.M., A.S. Cupp and J.R. Wood. 2018. Use of case-based or hands-on laboratory exercises with physiology lectures improves knowledge retention, but veterinary medicine students prefer case-based activities. Adv Physiol Educ. 42:182-191.