NC_OLD229: Porcine Reproductive And Respiratory Syndrome (PRRS): Mechanisms Of Disease And Methods For The Detection, Protection And Elimination of the

(Multistate Research Project)

Status: Inactive/Terminating

SAES-422 Reports

12/06/2002

Does not include abstracts, proceedings, or lay publications.



Bastos RG, Dellagostin O, Barletta R, Doster A, Nelson E, Lopez O, Osorio FA. 2002. Construction and immunological evaluation of M. bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus, Vaccine (In Press)



Batista L, Dee SA, Rossow KD, Deen J, Pijoan C. 2002. An assessment of PRRV persistence and shedding in a large population of breeding age female swine. Can J Vet Res 66:196-200.



Bautista EM, Faaberg, KS, MacGruder ED. 2002. Cloning and expression of PRRSV ORF1b and characterization of NTPase activity. Virology 298:258-270.



Benson JE, Yaeger MJ, Christopher-Hennings J, Lager K, Yoon K-J. 2002. A comparison of virus isolation, immunohistochemistry, fetal serology, and reverse transcriptase-polymerase chain reaction for the diagnosis of porcine reproductive and respiratory syndrome in the fetus. J Vet Diagn Invest 14:8-14.



Bierk MD, Dee SA, Rossow KD, Collins JE, Otake S, Molitor TW. 2001. Transmission of PRRS virus from persistently infected sows to contact controls. Can J Vet Res 65: 261-266.



Boettcher TB, Thacker BJ, Halbur PG, Waters WR, Nutsch R, Thacker EL. 2002. Vaccine efficacy and immune response to Mycoplasma hyopneumoniae challenge in pigs vaccinated against porcine reproductive and respiratory syndrome virus and M. hyopneumoniae. J Swine Hlth Prod 10:259-264.



Cancel-Tirado SM, Yoon K-J. 2002. Antibody dependent enhancement of virus infection and disease. Viral Immunol. (in press)



Chang CC, Yoon KJ, Zimmerman JJ, Harmon KM, Dixon PM, Dvorak CMT, Murtaugh MP. 2002. Evolution of porcine reproductive and respiratory syndrome (PRRS) virus during sequential passages in pigs. J Virol. 76:4750-4763.



Christopher-Hennings J, Faaberg KS, Mengeling WL, Murtaugh MP, Nelson EA, Roof MB, Vaughn EM, Yoon K-J, Zimmerman JJ. 2002. Porcine reproductive and respiratory syndrome (PRRS) diagnostics: Interpretation and limitations. J Swine Hlth Prod. 10:213-218.



Christopher-Hennings J, Holler LD, Benfield DA, Nelson EA. 2001. Detection and duration of porcine reproductive and respiratory syndrome virus in semen, serum, peripheral blood mononuclear cells and tissues from Yorkshire, Hampshire and Landrace boars. J Vet Diagn Invest. 13:133-142.



Cuatero L, Dee SA, Deen J, Ruiz A, and Pijoan C. 2002. Association between clinical signs and PRRSV viremia in nursery pigs under field conditions. Journal of Swine Hlthd Prod. 10:119-122.



Daginakatte GC, Kapil S. 2001. Fine mapping of the RNA binding domains of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. Adv Expt Med Biol. 494:547-552.



Dee SA, Deen J. 2001. Establishment of a PRRS ELISA-negative boar population using previously exposed boars. Vet Rec 149: 678-680.



Dee SA, Deen J, Rossow KD, Mahlum C, Otake S, Joo HS, C Pijoan. 2002. Mechanical transmission of porcine reproductive and respiratory syndrome virus throughout a coordinated sequence of events during cold weather. Can J Vet Res (In press).



Dee SA, Torremorell M, Rossow K, Mahlum C, Otake S, and Faaberg K. 2001. Identification of genetically diverse sequences (ORF 5) of PRRSV in a swineherd. Can J Vet Res. 65:254-260.



Fairbanks K, Chase C, Benfield DA. 2002. Tonsil biopsies and polymerase chain reaction assay for detection of breeding age gilts persistently infected with porcine reproductive and respiratory syndrome virus. J Swine Hlth Prod. 10:1-2.



Feng W, Laster SM, Tompkins M, Brown TT, Xu J-S, Gomez W, Benfield D, McCaw MB. 2002. Thymocyte and peripheral blood T lymphocyte subpopulation changes in piglets following in utero infection with porcine reproductive and respiratory syndrome virus. Virology 302:363-372.



Foss DL, Zilliox MJ, Meier W, Zuckermann F, Murtaugh MP. 2002. Adjuvant danger signals increase the immune response to porcine reproductive and respiratory syndrome virus. Viral Immunol. 15:557-566.



Halbur PG, Pallares FJ, Rathje JA, Evans R, Hagemoser WA, Paul PS, Meng XJ. 2002. Effects of different U.S. isolates of porcine reproductive and respiratory syndrome virus (PRRSV) on blood and bone marrow parameters of experimentally infected pigs. Vet Rec 151:344-348.



Hermann JR, Honeyman MS, Zimmerman JJ, Thacker BJ, Holden PJ, Chang CC. 2002. Effect of dietary Echinacea purpurea on viremia and performance in porcine reproductive and respiratory syndrome virus-infected nursery pigs. J An Sci (submitted).



Horter D, Chang CC, Pogranichnyy R, Zimmerman J, Yoon KJ. 2001. Persistence of porcine reproductive and respiratory syndrome in pigs. Adv Exp Med Biol. 494:91-94.



Horter DC, Pogranichniy RM, Chang C-C, Evans RB, Yoon K-J, Zimmerman JJ. 2002. Characterization of the carrier state in porcine reproductive and respiratory syndrome virus infection. Vet Microbiol. 86:213-228.



Hurd HS, Bush EJ, Losinger W, Corso B, Zimmerman JJ, Wills R, Swenson S, Pyburn D, Yeske P, Burkgren T. 2001. Outbreaks of porcine reproductive failure: report on a collaborative field investigation. J Swine Hlth Prod. 9:103-108.



Key KF, Haqshenas G, Guenette D, Swenson SL, Toth TE, Meng XJ. 2001. Genetic characterization of the major envelope gene of acute porcine reproductive and respiratory syndrome virus isolates. Vet Micro. 83:249-263.



Key KF, Guenette DK, Yoon KJ, Halbur PG, Vaughn EM, Roof M, Toth TE, Meng XJ. 2002. Identification and differentiation of vaccine-like isolates of porcine reproductive and respiratory syndrome virus from field isolates using a heteroduplex mobility assay. J Clin Micro. In press.



Kim TS, Benfield DA, Rowland RRR. 2002. Porcine reproductive and respiratory syndrome virus-induced cell death exhibits features consistent with a non-typical form of apoptosis. Virus Res. 85:133-140.



Kleiboeker SB, Lehman JR, Fangman TJ. 2002. Concurrent use of reverse transcription-polymerase chain reaction testing of oropharyngeal scrapings and paired serological testing for detection of porcine reproductive and respiratory syndrome virus in sows. J Swine Hlth Prod. 10:251-258.



Majhdi F, Kapil S. 2001. Interactions of cellular proteins with the positive strand of 3‘ untranslated RNA and the nucleoprotein gene of porcine reproductive and respiratory syndrome virus. Adv Expt Med Biol. 494:633-639.



Meier WA, Galeota J, OsorioFA, Husmann RJ, Schnitzlein W, Zuckermann FA. 2003. Gradual development of the interferon-gamma and antibody responses of swine to porcine reproductive and respiratory syndrome virus. Virology In press.



Murtaugh MP, Yuan S, Nelson EA, Faaberg KS. 2002. Genetic interaction between porcine reproductive and respiratory syndrome virus (PRRSV) strains in cell culture and in animals. J Swine Hlth Prod. 10:15-21.



Murtaugh MP, Foss DL. 2002. Inflammatory cytokines and antigen presenting cell activation. Vet Immunol Immunopathol. 87:109-122.



Murtaugh MP, Xiao Z, Zuckermann F. 2002. Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol. 15:533-547.



Murtaugh MP, Yaun S, Nelson EA, Faaberg KS. 2002. Genetic interaction between PRRSV strains in cell culture and in animals. J Swine Hlth Prod. 10:15-21.



Murtaugh MP, Xiao Z, Rutherford MS, Zuckermann F. 2002. Immunology. The Porcine Reproductive and Respiratory Syndrome (Porcine Arterivirus) Compendium (2nd Edition). Section 5.3. 28 pp. National Pork Board, Clive, Iowa. In press.



Nielsen HS, Liu GP, Nielsen J, Oleksiewicz MB, Bxtner A, Storgaard T, Faaberg KS. 2003. Generation of an infectious clone of VR-2332, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus. J Virol. In press.



Opriessnig T, Halbur PG, Yoon K-J, Pogranichniy RM, Harmon KM, Evans R, Key KF, Pallares FJ, Thomas P, Meng X-J. 2002. Comparative pathogenicity of a modified live PRRSV vaccine (Ingelvac PRRS MLV), the parent strain of the vaccine (ATCC VR2332), ATCC VR2385, and two recent field isolates of PRRSV. J Virol. 76:11837-11844.



Osorio FA, Galeota JA, Nelson E, Brodersen B, Doster A, Wills R, Zuckermann F, Laegreid WW. 2002. Passive transfer of virus -specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity. Virology 302:9-20.



Ostrowski M, Galeota JA, Jar AM, Platt KB, Osorio FA, Lopez OJ. 2002 .Identification of neutralizing and non-neutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol. 76:4241-50.



Otake S, Dee SA, Jacobson L, Torremorell M, and Pijoan C. 2002. Evaluation of aerosol transmission of porcine reproductive and respiratory syndrome virus under field conditions. Vet Rec. 150:804-808.



Otake S, Dee SA, Rossow KD, Deen J, Joo HS, Molitor TW, Pijoan C. 2002. Transmission of porcine reproductive and respiratory syndrome virus by fomites (boots and coveralls). J Swine Hlth Prod. 10: 59-65.



Otake S, Dee SA, Rossow KD, Deen J, Joo HS, Molitor TW, Pijoan C. 2002. Transmission of PRRSV by needles. Vet Rec. 150:114-115.



Otake S, Dee SA, Rossow KD, Moon RD, Pijoan C. 2002. Mechanical transmission of porcine reproductive and respiratory syndrome virus by mosquitoes, Aedes vexans (Meigen). Can J Vet Res. 66:191-195.



Otake S, Dee SA, Rossow KD, Moon RD, Trincado C, Pijoan C. 2003. Transmission of porcine reproductive and respiratory syndrome virus by houseflies (Linneaus). Vet Rec. In press.



Pallares FJ, Halbur PG, Opriessnig T, Sorden SD, Villar D, Janke BH, Yaeger MJ, Larson DJ, Schwartz KJ, Yoon KJ, Hoffman LJ. 2002. Porcine circovirus type 2 (PCV-2) co-infections in U.S. field cases of postweaning multisystemic wasting syndrome (PMWS). J Vet Diagn Invest. 14:515-519.



Pallares FJ, Halbur PG, Opriessnig T, Vaughn EM, Paul PS. 2002. Effects of dual infection of SPF pigs with low virulent isolates of porcine respiratory coronavirus (PRCV) and porcine reproductive and respiratory syndrome virus (PRRSV). Anaporc Cientifico 2:8-13.



Plagemann PGW, Rowland RRR, FaabergKS. 2002. The primary neutralization epitope of porcine reproductive and respiratory syndrome virus strain VR-2332 is located in the middle of the GP5 ectodomain. Arch Virol. 147:2327-2347.



Pogranichnyy RM, Yoon K-J, Harm PA, Sorden S, Daniels M. 2002. Case-control study on the association of porcine circovirus type 2 and other swine viruses in postweaning multisystemic wasting syndrome. J Vet Diagn Invest. 14:449-456.



Rowland R, Schneider P, Fang Y, Wootton S, Yoo D, Benfield DA. 2003. Peptide domains involved in the trafficking of the porcine reproductive and respiratory syndrome virus nucleocapsid protein. Virology. In press.



Wagstrom EA, Chang CC, Yoon KJ, Zimmerman JJ. 2001. Shedding of porcine reproductive and respiratory syndrome virus in mammary gland secretions of sows. Am J Vet Res. 62:1876-1880.



Wills RW, Doster AR, Galeota JA, Sur JH, Osorio FA. 2003. Duration of infection and proportion of pigs persistently infected with porcine reproductive and respiratory syndrome virus (PRRSV). J Clin Micro. In press.



Wills RW, Doster AR, Osorio FA. 2002. Transmission of porcine reproductive and respiratory syndrome virus (PRRSV) to age-matched sentinel pigs. J Swine Hlth Prod. 10:161-165.



Wootton SK, Rowland RR, Yoo D. 2002. Phosphorylation of the porcine reproductive and respiratory syndrome virus (PRRSV) nucleocapsid protein. J Virol. 76:10569-10576.



Yoon K-J, Christopher-Hennings J, Nelson EA. 2002. Diagnosis. In: Porcine Reproductive and Respiratory Syndrome (Porcine Arterivirus) Compendium (2nd Edition). National Pork Board, Clive, Iowa. In press.



Yoon K-J, Stevenson G. 2002. Porcine reproductive and respiratory syndrome: Diagnosis. In: Morilla A, Yoon KJ, Zimmerman JJ (eds). Trends in Emerging Viral Infections of Swine, pp 347-354. Iowa State University Press, Ames, Iowa



Yoon K-J. 2002. Porcine reproductive and respiratory syndrome: Virology. In: Morilla A, Yoon KJ, Zimmerman JJ (eds). Trends in Emerging Viral Infections of Swine, pp 339-346. Iowa State University Press, Ames, Iowa



Yoon K-J. 2002. Virology. In: The Porcine Reproductive and Respiratory Syndrome (Porcine Arterivirus) Compendium (2nd edition). Zimmerman JJ (ed). National Pork Board, Des Moines Iowa (in press).



Zimmerman J. 2002. Epidemiology. In: The Porcine Reproductive and Respiratory Syndrome (Porcine Arterivirus) Compendium (2nd edition). Zimmerman JJ (ed). National Pork Board, Des Moines Iowa (in press).



Zimmerman J. 2002. A short history of porcine reproductive and respiratory syndrome. In: The Porcine Reproductive and Respiratory Syndrome (Porcine Arterivirus) Compendium (2nd edition). Zimmerman JJ (ed). National Pork Board, Des Moines Iowa (in press).



Zimmerman JJ. 2002. Porcine reproductive and respiratory syndrome virus epidemiology. In: Morilla A, Yoon K-J, Zimmerman J (eds). Trends in Emerging Viral Infections of Swine. Iowa State Press, Ames, Iowa, pp. 331-337.

02/03/2004

Bastos, R.G., O.A. Dellagostin, R.G. Barletta, A.R. Doster, E.A. Nelson and F.A. Osorio. 2002. Construction and immumogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive and respiratory syndrome virus. Vaccine 21:21-29.



Batista L, Dee S, Molitor T, Olin M, Joo HS, Pijoan C, Murtaugh MP, Xiao Z, Rossow K, Polson D. 2004. Detection of porcine reproductive and respiratory syndrome virus in pigs with low positive or negative ELISA sample-to-positive ratios. Vet Rec. 154:25-26.



Batista L, Lwamba H, Pijoan C, Johnson CR, Murtaugh MP. 2004. Genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in two regions of Mexico. In press.



Cafruny, WA, QA Jones, TR Haven, NL Zitterkopf, PGW Plagemann and RRR Rowland. 2003. Glucocorticoid regulation of lactate dehydrogenase-elevating virus replication in macrophages, Virus Res, 92:83-87.



Cancel-Tirado SM, Yoon K-J. 2003. Antibody dependent enhancement of virus infection and disease. Viral Immunol 16:69-86



Dee SA, Deen J, Pijoan C. 2004. An evaluation of four intervention strategies to prevent mechanical transmission of porcine reproductive and respiratory syndrome virus. Can J Vet Res In press.



Dee SA, Deen J, Rossow KD, Eliason R, Mahlum C, Otake S, Joo HS, and Pijoan C. 2003. Mechanical transmission of porcine reproductive and respiratory syndrome virus throughout a coordinated sequence of events during warm weather. Can J Vet Res 67:12-16.



Dee SA. 2003. Approaches to prevention, control and eradication of PRRS. National Pork Board PRRS Compendium, 2nd edition, 119-130.



Feng, W-H, Laster, SM, Tompkins, M, Brown, TT, Xu, J-S, Gomez, W, Benfield, D, and McCaw, MB. 2002. Thymocyte and peripheral blood T lymphocyte subpopulation changes in piglets following in utero infection with porcine reproductive and respiratory syndrome virus. Virology 302:363-372.



Feng, W-H, Tompkins, M. Xu, J., Zhang, H-X., McCaw, MB. 2003. Analysis of constitutive cytokine expression by pigs infected in-utero with porcine reproductive and respiratory syndrome virus. Vet Immuno Immunopath. 94:35-45.



Ferrin, N.H., Y. Fang, C.R. Johnson, M.P. Murtaugh, D.D. Polson and E.A. Nelson. 2004. Validation of a blocking ELISA for the detection of antibodies against porcine reproductive and respiratory syndrome virus. Clinical and Diagnostic Laboratory Immunology. In press.



Foss, D.L., M.J. Zilliox, W. Meier, F. Zuckermann, and M.P. Murtaugh. 2002. Adjuvant danger signals increase the immune response to porcine reproductive and respiratory syndrome virus. Viral Immunol. 15:557-566.



Goldberg, T. L., J. F. Lowe, S. M. Milburn, L. D. Firkins. 2004. Quasispecies diversity of porcine reproductive and respiratory syndrome virus during natural infection. Virology. 317:197-207.



Hermann JR, Honeyman MS, Zimmerman JJ, Thacker BJ, Holden PJ, Chang CC. 2003. Effect of dietary Echinacea purpurea on viremia and performance in porcine reproductive and respiratory syndrome virus-infected nursery pigs. J An Sci 81:2139-2144.



Jiang Z, Zhou E-M, Ameri-Hahabadi M, Zimmerman JJ, Platt KB. 2003. Identification and characterization of auto-anti-idiotypic antibodies specific for antibodies against porcine reproductive and respiratory syndrome virus envelope glycoprotein (GP5). Vet Immunol Immunopathol 92:125-135.



Key K, Guenette DK, Yoon K-J, Halbur PG, Toth TE, Meng X-J. 2003. Identification and differentiation of vaccine-like isolates of porcine reproductive and respiratory syndrome virus from field isolates using a heteroduplex mobility assay. J Clin Microbiol 41:2433-2439



Kleiboeker, S.B., Lehman, J.R., and Fangman, T.J. 2002. Concurrent use of reverse transcription-polymerase chain reaction testing of oropharyngeal scrapings and paired serological testing for detection of porcine reproductive and respiratory syndrome virus in sows. J Swine Health Production 10:251-258.



Meier WA, Judy Galeota J, Osorio FA, Husmann R, Schnitzlein W, and Zuckermann FA. 2003. Gradual Development of the Interferon__ Response of Swine to Porcine Reproductive and Respiratory Syndrome Virus. Virol. 309:18-31



Murtaugh, M.P. Z. Xiao, and F. Zuckermann. 2002. Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol. 15:533-547.



Murtaugh, M.P., Z. Xiao, M.S. Rutherford and F. Zuckermann. 2003. Immunology. The PRRS (Porcine Arterivirus) Compendium (2nd Edition). Section 5.3. 28 pp. National Pork Board, Clive, Iowa.



Nielsen, H.S., Liu, G.-P., Nielsen, J., Oleksiewicz, M. B., Bxtner, A., Storgaard, T. and K.S. Faaberg. 2003. Generation of an infectious clone of VR-2332, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus. J. Virol., 77:3702-3711.



Opriessnig T, Halbur PG, Yoon K-J, Pogranichniy RM, Harmon KM, Evans R, Key KF, Pallares FJ, Thomas P, Meng X-J. 2002. Comparison of molecular and biological characteristics of a modified live PRRSV vaccine (Ingelvac PRRS MLV), the parent strain of the vaccine (ATCC VR2332), ATCC VR2385, and two recent field isolates of PRRSV. J Virol 76:11837-11844.



Osorio, F.A., J.A. Galeota, E.A. Nelson, B. Broderson, A. Doster, R. Wills, F. Zuckermann and W.W. Laegreid. 2002. Passive transfer of virus-specific neutralizing antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity. Virology, 302:9-20.



Otake S, Dee SA, Moon RD, Rossow KD, Trincado C and Pijoan C. 2003. Evaluation of mosquitoes, Aedes vexans, as biological vectors of porcine reproductive and respiratory syndrome virus. Can J Vet Res 67:265-270.



Otake S, Dee SA, Moon RD, Rossow KD, Trincado C, and Pijoan C. 2003. Transmission of porcine reproductive and respiratory syndrome virus by houseflies (Musca domestica). Vet Rec 152:73-76.



Otake S, Dee SA, Moon RD, Rossow KD, Trincado C, Farnham M, and Pijoan C. 2003. Survival of porcine reproductive and respiratory syndrome virus in houseflies . Can J Vet Res 67:198-203.



Reilly, C., C. Wang, and M.S. Rutherford, 2003. A method for the normalizing microarrays using the genes that are not differentially expressed. J. Amer. Statistical Assoc., in press.



Ropp, S. L., Mahlum Wees, C. E., Fang, Y., Nelson, E. A., Rossow, K. D., Bien, M., Arndt, B., Preszler, S., Steen, P., Christopher-Hennings, J., Collins, J. E., Benfield, D. A., and K. S. Faaberg 2003. Characterization of emerging European-like PRRSV isolates in the United States. J. Virol., accepted for publication.



Rowland, R.R.R., S. Lawson, K. Rossow and D. Benfield. 2003. Lymphoid tissue tropism of porcine reproductive and respiratory syndrome virus replication during persistent infection of pigs originally exposed to virus in utero. Vet Microbiol. 96:219-235.



Rowland, RRR and D Yoo. 2003. Nucleolar-cytoplasmic shuttling of the PRRSV nucleocapsid protein: a simple case of molecular mimicry or the complex regulation by nuclear import, nucleolar localization and nuclear export signal sequences. Virus Res, 95:23-33.



Rowland, RRR, P Schneider, Y Fang, S Wootton, D Yoo, and DA Benfield. 2003. Peptide domains involved in the localization of the porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus. Virology 316:135-145.



Thanawongnuwech, R., Young, T.F., Thacker, B.J., Halbur, P.G., Thacker, E.L. 2003. Interleukin (IL) 10, IL 12, and interferon gamma levels in the respiratory tract following Mycoplasma hyopneumoniae and PRRS infection in pigs. Viral Immunol., 16:357-367,



Wills R.W., Doster AR, Galeota JA, Sur JH, Osorio FA. 2003. Duration of infection and proportion of pigspersistently infected with porcine reproductive and respiratory syndrome virus (PRRSV). J Clin Micro 41:58-62.



Xiao Z, Batista L, Dee S, Halbur P, Murtaugh MP. 2004. The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of the virus load. J. Virol. In press.



Yoo D, SK Wootton, G Li, C Song and RRR Rowland. 2003. Colocalization and interaction of the porcine arterivirus nucleocapsid protein with the small nucleolar RNA-associated protein fibrillarin. J. Virol. 77:12173-12183.



Yoon K-J, Christopher-Hennings J, Nelson E. 2003. Diagnosis of PRRS. In: J.J. Zimmerman, K.-J. Yoon and E. Neumann (eds). 2003 PRRS Compendium: A reference for pork producers, pp. 55-67. National Pork Board, Des Moines, Iowa.



Yoon K-J, Christopher-Hennings J, Nelson E. 2003. Diagnosis. In: J.J. Zimmerman JJ and K.-J. Yoon (eds). 2003 PRRS Compendium: A comprehensive reference on PRRS for pork producers, veterinary practitioners, and researchers (2nd edition), pp 59-74. National Pork Board, Des Moines, Iowa.



Yoon K-J, Stevenson G. 2002. Porcine reproductive and respiratory syndrome: Diagnosis. In: A. Morilla, K.-J. Yoon and J.J. Zimmerman (eds). Trends in emerging viral infections of swine, pp 347-354. Iowa State University Press, Ames, Iowa



Yoon K-J. 2002. Porcine reproductive and respiratory syndrome: Virology. In: A. Morilla, K.-J. Yoon and J.J. Zimmerman (eds). Trends in emerging viral infections of swine, pp 339-346. Iowa State University Press, Ames, Iowa



Yoon K-J. 2003. Virology. In: J.J. Zimmerman JJ and K.-J. Yoon (eds). 2003 PRRS Compendium: A comprehensive reference on PRRS for pork producers, veterinary practitioners, and researchers (2nd edition), pp 163-184. National Pork Board, Des Moines, Iowa.



Yuan S, Murtaugh MP, Schumann FA, Mickelson D, and Faaberg KS. 2003. Characterization of heteroclite subgenomic RNAs associated with PRRSV infection. Virology. In press.



Zhou E-M, Jiang Z, Ameri-Mahabadi M, Zimmerman JJ. 2003. Detection and characterization of auto-anti-idiotypic antibodies specific for Idiotypic antibodies against envelope glycoprotein and matrix protein of porcine reproductive and respiratory syndrome virus. J Virol Methods (in press).



Zimmerman J. Epidemiology and ecology. 2003. In: The Porcine Reproductive and Respiratory Syndrome Compendium: A comprehensive reference on PRRS for pork producers, veterinary practitioners, and researchers (2nd edition). Zimmerman JJ, Yoon K-J (eds). National Pork Board, Des Moines Iowa, pp.27-50.



Zimmerman J. Historical overview. 2003. In: The Porcine Reproductive and Respiratory Syndrome Compendium: A comprehensive reference on PRRS for pork producers, veterinary practitioners, and researchers (2nd edition). Zimmerman JJ, Yoon K-J (eds). National Pork Board, Des Moines Iowa, pp.1-6.



Zimmerman JJ, Yoon K-J (editors). 2003. PRRS Compendium: A comprehensive reference on PRRS for pork producers, veterinary practitioners, and researchers (2nd edition). National Pork Board, Des Moines, Iowa. 294 pages



PROCEEDINGS AND ABSTRACTS:



Callahan, J.D., J. Christopher-Hennings, T.A. Gay, M.E. Reos, Y.Fang, M. Dammen, A. Wasilk, J. Galeota, F.A. Osorio, M. Torremorell, W.M. Nelson and E.A. Nelson. 2003. Development, validation and commercialization of a real-time RT-PCR assay for the detection of Lelystad, European-like and US Porcine reproductive and respiratory syndrome viruses. 107th Annual Meeting of the American Assoc. of Vet. Lab. Diagnostitions, p. 142.



Carlson, D.L., A.N. Wasilk, Y. Fang, S.A.E. Marras, E.A. Nelson and J. Christopher-Hennings. 2002. Detection, differentiation and quantitation of PRRSV strains by real-time RT-PCR with molecular beacons. 83rd Conf. of Research Workers in Animal Disease.



Christopher-Hennings, J., J. Callahan, T. Gay, A. Wasilk, Y. Fang, M. Dammen, M. Torremorell, D. Polson, M. Mellencamp, E.A. Nelson and W.M. Nelson. 2003. Validation of a real-time, quantitative PCR assay to detect U.S. and Lelystad/European-like PRRSV in boar semen and serum. 84th Conf. of Research Workers in Animal Disease.



Faaberg KS, Murtaugh MP, Mahlum-Wees CE, Johnson JE, Dwan C. 2003. Evolution of ORF5 in North America over 15 years. Proc. IXth International Symposium of Nidoviruses. 4.4, Egmond aan Zee, The Netherlands.



Faaberg, K. S., Murtaugh, M. P., Mahlum-Wees, C. E., Johnson, J. E., and C. Dwan. 2003. PRRSV: Evolution of ORF5 in North America over 15 years. IXth International Symposium on Nidoviruses,



Fang, Y., D. Kim, R.R.R. Rowland, S. Ropp, P. Steen, J. Christopher-Hennings and E.A. Nelson. 2003. Heterogeneity in the NSP2 gene of European-like PRRSV isolated in the United States. 84th Conf. of Research Workers in Animal Disease.



Fang, Y., W.P. Zhang, R.R.R. Rowland, J. Christopher-Hennings and E.A. Nelson. 2003. Phylogeny of European-like PRRSV isolates in North America. 84th Conf. of Research Workers in Animal Disease.



Ferrin, N.H., Y. Fang, D.D. Polson, M. Torremorell, M. Gramer, M.P. Murtaugh, C.R. Johnson, and E.A. Nelson. 2002. Validation of a blocking ELISA for antibodies against PRRSV. 83rd Conf. of Research Workers in Animal Disease.



Goldberg, T. L., J. F. Lowe, S. M. Milburn, L. D. Firkins. 2003. Quasispecies diversity of porcine reproductive and respiratory syndrome virus during natural infection. CRWAD 2003 Proceedings. ISU Press. Abst 153.



Hadley, L., Xu, J-S., McCaw, M.B., and Laster, S. 2002. Purified proteins from an acute strain of PRRSv provide protection via TH1-type immune response. In: Proceedings of 17th Congress of the International Pig Veterinary Society, Ames, Iowa, USA.



Liu, G., Nielsen, H. S., Burkhart, K. M., Roof, M. B., Vaughn, E. M., and K. S. Faaberg. 2003. Porcine Respiratory and Reproductive Syndrome Virus (PRRSV) Infectious Clones Reveal Nucleotides Important for Genome Replication. IXth International Symposium on Nidoviruses, P.6.6, Egmond aan Zee, The Netherlands.



Liu, G., Nielsen, H. S., Zhiming, O., and K. S. Faaberg. 2003. Infectivity of porcine respiratory syndrome virus genomic clones. 21th Annual Meeting of the American Society for Virology, W14-5, Davis, CA.



Lowe J.F., L.D. Firkins, F. A. Zuckerman, T.L. Goldberg. 2003. Immune response and reproductive performance in swine following known infection with PRRS virus in commercial herds. CRWAD 2003 Proceedings. ISU Press. Abst 144.



McCaw, M.B. Roberts, J, Laster, S.M., Hadley, L., and Erickson, G.A. 2003. Characterization of PRRSv antibody and rtPCR responses following repeated exposures and then challenge with homologous wild-type virus. In: Proceedings of 4th International Symposium on Emerging and Re-emerging Pig Diseases, Rome, Italy. pp 81-82.



McCaw, M.B., J. Roberts, J.J. Zimmerman, Laster, S., Hadley, L.,G.A. Erickson. 2002. PRRSv ELISA response and PCR viremia following repeated homologous exposures to wild-type virus in adult swine. Conference in Research Workers in Animal Disease. St. Louis, MO.



McCaw, M.B., Roberts, J., Laster, S., Hadley, L., and Erickson, G.A. 2002. PRRSv serologic assay limitations following repeated homologous exposures to wild-type virus in adult swine. In: Proceedings of 17th Congress of the International Pig Veterinary Society, 2002, Ames, Iowa, USA. p208.



Mondaca-Fernandez E, Morrison RB, Murtaugh MP. 2003. Using GIS and sequencing to assess regional epidemiology of PRRSV. Proc. Allen D. Leman Swine Conf. 30:71-74.



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