NC1035: Practical Management of SCN and Other Nematodes of Regional Importance: With Special Reference to Invasive Biology

(Multistate Research Project)

Status: Inactive/Terminating

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SAES-422 Reports

Annual/Termination Reports:

[11/06/2012] [05/01/2008] [04/23/2009] [09/25/2009] [08/30/2010] [08/11/2011]

Date of Annual Report: 11/06/2012

Report Information

Annual Meeting Dates: 12/18/2006 - 08/16/2012
Period the Report Covers: 10/01/2006 - 09/01/2011

Participants

Faghihi, Jamal (jfaghihi@purdue.edu) - Purdue University;
MacGuidwin, An (aem@plantpath.wisc.edu) - University of Wisconsin;
Pueppke, Steve (Pueppke@msu.edu) - Administrative Advisor;
Niblack, Terry (niblack.2@osu.edu) The Ohio State;
Robbins, Robert (rrobin@uark.edu) - University of Arkansas;
Todd, Tim (nema@ksu.edu) - Kansas State University;
Tylka, Greg (gltylka@iastate.edu) - Iowa State University;
Welacky, Tom (tom.welackyt@agr.gc.ca) - Agriculture and Agri-Food Canada;
Chen, Senyu (chenx099@umn.edu) - University of Minnesota;
Donald, Pat (pat.donald@ars.usda.gov) USDA;
Ferris, Virginia (vferris@purdue.edu) - Purdue University;
Melakeberhan, Hadish (melakebe@msu.edu) - Michigan State University;
Neher, Deborah (dneher@uvm.edu) - University of Vermont;
Powers, Tom (tpowers@unlnotes.unl.edu) - University of Nebraska

Participants Attachment:

Attachment

Brief Summary of Minutes

Accomplishments

Short-term outcomes: Improved use of soybean varieties resistant to the soybean cyst nematode (SCN) resulting in higher yields and decreased population densities of SCN. More unbiased information on SCN resistant varieties and soybean yield in relation to SCN population density is available. Data on H. glycines virulence phenotypes across the region were shared. Outputs: Publications as reported in the "Publications" section. Michigan presented a reference handbook for growers, Soybean Cyst Nematode (SCN) Managementby D. Brown-Rytlewski, G. Bird, F. Warner and K. Barnett, was presented to the committee for discussion. The Michigan publication was submitted as part of the NC1035 project objective to develop decision-support data bases for management of SCN for the grower community Activities: Objective One: Virulence profiles for SCN populations were conducted in Illinois, Indiana, Minnesota, Ontario, Tennessee, and Wisconsin. Host resistance for managing SCN was evaluated in Ontario, Kansas, Tennessee, Michigan, Minnesota, Illinois, and Iowa. Funding for this activity was obtained from the Tennessee Soybean Promotion Board and Ontario Soybean Board. Selection pressure imposed by continuous planting of SCN-resistant varieties was studied in Arkansas, Kansas, and Wisconsin. The impact of soil disturbance and tillage on SCN was determined in Indiana, Minnesota, Tennessee, and Vermont. Soil amendments for the management of SCN were evaluated in Ontario and Minnesota. The interaction of SCN with other pests and pathogens was studies in Illinois, Indiana, Iowa, Minnesota, Ontario, and Wisconsin. Alternative host studies were conducted in Indiana and Ontario. Community structure studies were conducted in Michigan, Vermont, and Tennessee. Rotations of soybean cultivars derived from different sources of resistance were compared. Objective Two: Surveys for SCN to identify candidate fields for Tier II activities were conducted in Illinois, Michigan, Minnesota, Nebraska, North Dakota, Ontario, and Wisconsin. Field results from candidate fields for Tier II activities were compared. Funding for this objective was secured for Minnesota and Vermont. A two year multi-state experiment on soil suppressiveness was conducted. Objective Three: An SOP for screening resistant lines to SCN was developed. Lists of SCN-resistant varieties available commercially were compiled by Iowa, Illinois, Ontario, and Tennessee and provided to soybean producers. Milestones: Data on corn nematodes was presented to corn pathologists to further cooperation between the two groups. An extensive dataset consisting of 3 years of observations on SCN resistance reactions among commercial soybean cultivars and the diversity of SCN virulence phenotypes has been compiled for the North Central Region. The prevalence of SCN populations that are virulent on PI 88788, the most common source of resistance, has been documented.

Publications

[2008] Avendano, F. M. E. O'Neal, and G. L. Tylka. 2007. Soybean cyst nematode and soybean aphid interactions on soybean. Journal of Nematology 39:85. Bernard, E. C., E. C., Donald, P. A., Handoo, Z. A. Heinz, R. D., and Powers, T. O. 2007. Characterization of a new species of cyst nematode parasitizing corn. Journal of Nematology 39:74. Bird, G. W. 2007. A Global Perspective of Integrated Nematode Management Innovation. Phytopathology 97:S148. Bird, G. W. 2007. Nematode community structure of natural, non-managed and managed ecosystems. Journal of Nematology 39:89. Chen, S. Y. 2007. Suppression of Heterodera glycines in soils from fields with long-term soybean monoculture. Biocontrol Science and Technology 17:125-134. Chen, S. Y. 2007. Tillage and crop sequence effects on Heterodera glycines and soybean yields. Agronomy Journal 98:897-907. Chen, S. Y., J. E. Kurle, S. R. Stetina, D. R. Miller, G. A. Nelson, L. D. Klossner, and N. C. Hansen. 2007. Interactions between iron-deficiency chlorosis and soybean cyst nematode in Minnesota soybean fields. Plant and Soil 299:131-139. Chen, S. and Liu, S. F. 2007. Effects of tillage and crop sequence on parasitism of Heterodera glycines juveniles by Hirsutella spp.and on juvenile population density. Nematropica 37:93-106. Chen, S., C. C. Sheaffer, D. L. Wyse, P. Nickel, H. Kandel, and C. M. Fernholz. 2007. Distribution and frequency of Heterodera glycines and other plant-parasitic nematodes in the organic-farming fields in Minnesota. Journal of Nematology 39:99. Creech, J. Earl, J. S. Webb, B. G. Young, J. P. Bond, S. K. Harrison, V. R. Ferris, J. Faghihi, A. Westphal, and W. G. Johnson. 2007. Development of soybean cyst nematode (Heterodera glycines) on henbit (Lamiium amplexicaule) and purple deadnettle (L. purpureum) 2007. Weed Technology 21:1064-1070. Creech, J. Earl, A. Westphal, V. R. Ferris, J. Faghihi, T. J. Vyn. J. B. Santini, and W. O. Johnson. 2007. Influence of winter annual weed management and crop rotation on soybean cyst nematode (Heterodera glycines) and winter annual weeds. Weed Science 56:103-111. Davenport, J., G. Bird and F. Warner. 2007. Dynamics of HG Types Associated with Commercial soybean cultivars in Michigan. Journal of Nematology 39:90. Donald, P. A., T. Kilen, and A. Mengitsu. 2007. Registration of soybean germplasm line D99-2018 resistant to Phytophthora rot (Rps6) and soybean cyst nematode races 3 (Hg Type 0) and 14 (Hg Type 1.3.6.7). Crop Science 47:451-452. Donald, P. A., Hayes, R., and Walker, E. 2007. Potential for soybean cyst nematode reproduction on winter weeds and cover crops in Tennessee. Online. Plant Health Progress do8i:1094/PHP-2007-0226-01-RS. Donald, P. A., and Tyler, D. D. 2007. Long- and short-term tillage effects on Heterodera glycines reproduction. Journal of Nematology 39:72. Andresen, D., J., G. Bird, J. Flore, L. Gut, P. McManus, J. Nugent, R. Isaacs, A. Schilder, M. Whalon, R. Sirrine, J. Sanchez. 2007. Tart Cherry Systems, pp. 74-101 (in) Ecologically Based Farming Systems, Deming, S., L. Johnson, D. Lehnert, D. Mutch, L. Probyn, K. Renner, J. Smeenk, S. Thalmann and L. Worthington (eds). Michigan State University Extension Bulletin E-2983. Lansing, 140 pp. Faghihi, J., S. Conley, and V. R. Ferris. 2007. Field reaction of selected SCN resistant soybean germplasm toward SDS. Journal of Nematology 39:76. Gao, X., A. L. Colgrove, K. N. Lambert, and T. L. Niblack. 2007. A molecular assay for reliable quantification of soybean cyst nematode in plants. Journal of Nematology 39:94. Gavassoni, W. L., G. L. Tylka, and G. P. Munkvold. 2007. Relationships among tillage practices, dissemination, and spatial patterns of Heterodera glycines and soybean yield. Plant Disease 91:973-978. Masler, E. P., P. A. Donald, S. Sardanelli. 2008. Stability of Heterodera glycines (Tylenchida:Heteroderidae) juvenile hatching from eggs obtained from different sources of soybean, Glycine max. Nematology. 10:271-278. Mebrahtu, T., Devine, T. E., Donald, P. A., and Abney, T. S. 2007. Registration of Owens vegetable soybean. Journal of Plant Registration 1:95-96. Melakeberhan, H., Mennan, S., Chen, S., Darby, B., and Dudek, T. 2007. Integrated approaches to understanding and managing Meloidogyne hapla populations parasitic variability. Crop Protection 26:894-902. Mengistu, A., Kilen, T. C., and Donald, P. A. 2007. Registration of D95-5048 soybean germplasm line resistant to Phytophthora rots and soybean cyst nematode races 3 and 14. Crop Science 47:452. Mennan, S., Chen, S. Y., and Melakeberhan, H. 2007. Effects of Hirsutella minnesotensis and N-Viro soil on populations of Meloidogyne hapla. Biocontrol Science and Technology 17:233-246. Mock, V. A., J. E. Creech, Bill Johnson, Jamal Faghihi, V. R. Ferris, A. Westphal, K. Bradley. 2007. Winter Annual Weeds and Soybean Cyst Nematode Management. Purdue Extension Publication. Niblack, T. L., Colgrove, K., and Bond, J. P. 2008. Shift in virulence of soybean cyst nematode is associated with use of resistance from PI 88788. Online. Plant Health Progress doi:10.1094/PHP-2008-0118-01-RS. Pedersen, P. and G. Tylka. 2007. Soybean cyst nematode and nematicide affect growth patterns of various soybean varieties in Iowa. Journal of Nematology 39:98. Schroeder, N. E., and A. E. MacGuidwin. 2007. Incorporation of a fluorescent compound by live Heterodera glycines. Journal of Nematology 39:3-49. Snapp, S, D. Smucker, R. Chase, W. Kirk, G. Bird and D. Douches. 2007. Michigan Potato Systems, pp. 60-73 (in) Ecologically Based Farming Systems, Deming, S., L. Johnson, D. Lehnert, D. Mutch, L. Probyn, K. Renner, J. Smeenk, S. Thalmann and L. Worthington (eds). Michigan State University Extension Bulletin E-2983. Lansing, 140 pp. Sun, M., S. Chen, J. E. Kurle, S. Naeve, D. L. Wyse, LO. A. Stahl, G. A. Nelson, and L. D. Klossner. 2007. Effect of rotation crops on vesicular-arbuscular mycorrhizal fungi and iron-deficiency chlorosis of soybean. Phytopathology 97:S113. Tabor, G. M., G. L. Tylka, and C. R. Bronson. 2007. Genotypes A and B of Cadophora gregata differ in ability to colonize susceptible soybean. Plant Disease 91:574-580. Tabor, G. M., G. L. Tylka, and C. R. Bronson. 2007. Genotype B of Cadophora gregata reduces quantity and quality of soybean yield. Phytopathology 97:S113. Xiang, M. C., Liu, X. Z., Xiao, Q. M., and Chen, S. Y. 2007. Nutritional requirements of the nematophagous fungus Hirsutella minnesotensis. Acta Phytopathologica Sinica 37:151-168. Xiang, M. C., Wang, Z. X., Liu, X. Z., Yang, X. H., Chen, S. Y., and Ziao, Q. M. 2007. Variability of morphology, parasitism, and nucleotide sequences among isolates and species of nematophagous Hirsutella. Biological Control 33:110-119. Xiao, J., J. Zhu, S. Chen, W. Ruan, and C. Miller. 2007. A novel use of anaerobically digested liquid swine manure to potentially control soybean cyst nematode. Journal of Environmental Science and Health Part C- - Pesticides Food Contaminants and Agricultural Wastes 42:749-757. Xiao, J., J. Zhu, S. Chen, W. Ruan, and C. Miller. 2007. Control of the soybean cyst nematode using anaerobically digested liquid swine manure. Journal of Nematology 39:73. Zasada, I., F. Avendano, S. Koenning, Y. Li, T. Logan, H. Melakeberhan, and G. Tylka. 2008. Potential of an alkaline-stabilized biosolid to manage nematodes: case studies on soybean cyst and root-knot nematodes. Plant Disease 92:4-13. [2009] Donald, P.A., Patananan, A., Cerna, C.S., Simmons, T., Castro, N., Wilgar, J.L., and Goheen, S.C. 2008. Protein SDS-PAGE characterization and comparison of soybean cyst nematodes. Nematropica 38: 137-144. Donald, P.A., Tyler, D.D., and Boykin, D. 2009. Short and long-term tillage effects on Heterodera glycines reproduction in soybean monoculture in west Tennessee. Soil and Tillage Research 104:126-133. Melakeberhan. H., S. Mennan, M. Ngouajio, and T. Dudek (2008). Effect of Meloidogyne hapla on multi-purpose use oilseed radish (Raphanus sativus). Nematology 10:375-379. Melakeberhan, H. (2008). Nutrient use efficiency and precision management of nematodes-Concepts and possibilities. Nematologia Brasileira 32:1-12. Niblack, T.L., Tylka, G.L., Arelli, P., Bond, J., Diers, B., Donald, P., Faghihi, J., Ferris, V.R., Gallo, K., Heinz, R.D., Lopez-Nicora, H., Von Qualen, R., Welacky, T., and Wilcox, J.A. 2009. A standard greenhouse method for assessing soybean cyst nematode resistance in soybean:SCE08 (Standardized Cyst Evaluation 2008). Online. Plant Health Progress doi:10.1094/PHP-2009-0513-01-RV. Poromarto, S. H, and Nelson, B. D. 2009. Reproduction of soybean cyst nematode on dry bean cultivars adapted to North Dakota and northern Minnesota. Plant Disease 93:507-511. Poromarto, S., and Nelson B. D. 2009. Crops hosts of soybean cyst nematode in the northern Great Plains. Phytopathology 99:S103-104. Poromarto, S. and Nelson, B. 2008. Effects of soybean cyst nematode on growth of dry bean. Phytopathology 98:S126. Melakeberhan, H., M. F. Avendano (2008). Spatio-temporal consideration of soil conditions and site-specific management of nematodes. Precision Agriculture 9:341-354. Zasada, I., M. F. Avendano, Li, Y. C., T. Logan, H. Melakeberhan., S. R. Koenning, G. L. Tylka (2008). Potential of alkaline-stabilized biosolid to manage nematodes: Case studies on soybean cyst and root-knot nematodes. Plant Disease 92:4-13. Technical: Brown-Rytlewski, D., G. Bird, F. Warner and K. Barnett, 2009. Soybean Cyst Nematode (SCN) Management A field guied for SCN management in Michigan. North Central Soybean Research Program and Michigan Soybean Checkoff. Michigan State University Extension, 27 p. Mock, V. A. J. E. Crech, W. Johnson, J. Faghihi, V. R. Ferris, A Westphal, K. Bradley. 2007. Winter Annual Weeds and Soybean Cyst Nematode Management. (With a guide for identifying known weed hosts). Purdue University Extension WS-36. Melakeberhan. H. (2008). Roles for nematology in understanding renewable energy needs and balancing food and socio economic interests. 5th International Congress of Nematology, Brisbane Australia. 117 Melakeberhan. H., A. Kravchenko, and M. Ngouajio (2008). Strategies for enhancing brassicas multipurpose attributes in managing nematode parasitism complexities. 3rd International Biofumigation Symposium, Canberra, Australia. 37. Welacky, T.W., T. Anderson, C. Fox, D. Hunt, and R. Michellutti. 2009. Effect of Riparian Hedgrows on beneficial organisms and economic pests in Ontario. Society of Nemotologists 48th Annual Meeting, Burlington Vermont. p 49. [2010] Journal Articles Delheimer, J. C., T. Niblack, Schmidt, M. Shannon, G., and B. W. Diers. 2010. Comparison of the effects in field tests of the SCN resistance genes from different resistance sources. Crop Science 50:2331-2239. Faghihi, Jamal, P. A. Donald, G. Noel, T. W. Welacky, and V. R. Ferris. Soybean resistance to field populations of Heterodera glycines in selected geographic areas. Plant Health Progress. doi:10.1094/PHP-2010-D426-01-RS. Mekete, T., M. E. Gray, and T. L. Niblack. 2009. Distribution, morphological description, and molecular characterization of Xiphenema and Longidorus spp. associated with plants (Miscanthus spp. and Panicum virgatum) used for biofuels. GCB Bioenergy 1:257-266. doi: 10.1111/j.1757-1707.2009.01020.x. Neher, D.A. 2010. Ecology of plant and free-living nematodes in natural and agricultural soil. Annual Review of Phytopathology 48:371-394. Poromarto, S. H., and Nelson, B. D. 2010. Evaluation of northern-grown crops as hosts of soybean cyst nematode. Online. Plant Health Progress doi:10.1094/PHP-2010-0315-02-RS. Rogovsa, N., F.W. Blackmer, and G.L. Tylka. 2009. Soybean yield and soybean cyst nematode densities related to soil pH, soil carbonate concentrations, and alkalinity stress index. Agronomy Journal 101:1019-1026 (also online doi:10.2134/agronj2008.0086x). Studham, M., G.C. MacIntosh, F. Avendaño, D. Soh, and G.L. Tylka. 2009. The soybean resistance gene Rag1 does not protect against soybean cyst and root-knot nematodes. Online. Plant Health Progress doi:10.1094/PHP-2009-0401-01-BR. Zheng, J., Zhang, Y., Li, X., Zhao, L., and Chen, S. 2009. First report of the soybean cyst nematode, Heterodera glycines, on soybean in Zhejiang, Eastern China. Plant Disease 93:319. Abstracts Chen, S., and Zheng, J. 2009. Fast loss of resistance in cultivars with current sources of resistance: A challenge in soybean cyst nematode management. World Soybean Research Conference VIII Proceedings (2009) Online http://www.wsrc2009.cn/en/index.asp. Donald, P.A. and Hewlett, T. 2009. Pasteuria nishizawae studies in Tennessee. Journal of Nematology 41:325. Faghihi, J., Donald, P., Welacky, T., Noel, G., and Ferris, V. 2009. Geographic differences in field populations of soybean cyst nematode (SCN). Journal of Nematology 41:327. Giesler, L.J., C. Bradley, A. Dorrance, T. Niblack, G. Tylka, D. Jardine, D. Malvick, L. Sweets, S. Markell, L. Osborne, P. Esker, G. Bird, J. Faghihi, and A. Tenuta. 2009. Improving management of soybean cyst nematode through extension demonstration and outreach. Proceedings of the 2009 North Central Division Meeting of the American Phytopathological Society. p. 21. Lopez Nicora, H.D., J. P. Craig, and T. L. Niblack. 2009. A method to assess infection of soybean roots by soybean cyst nematode with quantitative polymerase chain reaction. Phytopathology 99:S76. Lopez-Nicora, H.D., J. Morrison, D. Feltes, and T. L. Niblack. 2009. Distribution and densities of plant-parasitic nematodes on Zea mays in northern Illinois. Journal of Nematology 41;350. Mengistu, T. M., and T. Niblack. 2009. Distribution, morphological description and molecular characterization of Pratylenchus spp. associated with biofuel crops. Phytopathology 99:S84. Melakeberhan, H. (2009a). Managing nematode parasitic variability: case studies of soybean cyst nematode and root-knot nematodes. 19th Symposium of the Nematological Society of Southern Africa Proceedings. 25. Melakeberhan, H. (2009b). Application of fertilizer-use efficiency model changing and managing soil conditions in agro-biologically integrated ways. 19th Symposium of Nematological Society of Southern Africa Proceedings. 49. Melakeberhan, H. (2009c). Cross-disciplinary efficiency assessment of soil amendments. Proceeding of the Joint Meeting of the Society of Nematologists and Soil Ecology Society. 146. Melakeberhan, H. (2009d). Fertilizer use efficiency model for managing nematodes and plant, soil and environmental health. SSSA, S-4. CD-Rom. Melakeberhan, H. (2009e). Perspectives for nematology education in the 21st Century: Lessons from the past to make the future better. Proceeding of the Joint Meeting of the Brazilian Society of Nematologists and Society of Tropical American Nematologists. CD-Rom. Poromarto, S. H., and Nelson, B. D. 2010. Effects of soybean cyst nematode on growth of kidney and navy bean. Phytopathology 100:S102 (Abstract) Poromarto, S. H., and Nelson, B. D. 2010. Lack of adaptation toward greater reproduction of soybean cyst nematode on dry bean. Phytopathology 100:S102 (Abstract) Rudolph, K., Bolton, M. D., and Nelson, B. D. 2010. Soybean cyst nematode infects roots of sugar beet. Phytopathology 100:S111 (Abstract) Tylka, G.L., G.D. Gebhart, and C. C. Marett. 2009. Performance of SCN-resistant soybean varieties in fields infested with different soybean cyst nematode HG types. Proceedings of the 2009 North Central Division Meeting of the American Phytopathological Society. pp. 28-29. Zheng, J. W. and Chen, S. Y. 2009. Estimating virulence of soybean cyst nematode field populations in response to use of resistant cultivars. Society of Nematologists 48th Annual Meeting & Soil Ecology Society 12th Biennial Meeting Abstracts:212. (Abstr.). Research Reports: Cary, T. R. and Diers, B.SCN resistance ratings, in the Variety Information Program for Soybeans (VIPS) at www.vipsoybeans.org 2010 Northern Regional Soybean Cyst Nematode Test report, Group I-IV. Urbana, IL : University of Illinois, Dept. of Crop Sciences. Johnson, Allen, F.L., R., Williams, R.C., Thompson, A., Newman, M., Donald, P. 2009. Soybean variety performance tests in Tennessee. http://varietytrials.tennessee.edu/soybean.htm Newman, M. et al.. 2009 Soybean disease ratings and yields 2009 test summaries. University of Tennessee Extension. http://www.utextension.utk.edu/fieldCrops/soybean/diseases/disease_images/2009-Soybean-Report.pdf Welacky, T.W. 2010 Report - Ontario Soybean Variety Trials. Ontario Oil and Protein Seed Crop Committee. 12p. Producer and editor of internet site, http://www.gosoy.ca with graphs, % Protein and % Oil reports and tables of varietal responses to SCN and various diseases in Ontario (3,000 visits annually). Soy Data editor and production co-coordinator of interactive internet web site for seed sponsors and test cooperators to organize entry information for yearly Ontario Soybean Variety Trial plots across 20 locations in Ontario, Quebec and Manitoba. Hager, H. 2009. Breakdown of Soybean Resistance to Soybean Cyst Nematode. Top Crop Manager East, January, 2009, p. 6-7. Extension Publications: Tylka, G.L. 2009. Soybean cyst nematode-resistant soybean varieties for Iowa. Iowa State University Extension Publication PM 1649, 24 pp. Tylka, G.L., G.D. Gebhart, and C.C. Marett. 2009. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa 2008. Iowa State University Extension Publication IPM 52, 26 pp. Tylka, G. 2009. Quick facts about corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) - 28 April 2009. Tylka, G. 2009. Spring sampling not recommended for most corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) - 7 May 2009. Tylka, G. 2009. SCN and Corn Nematode Workshops set for July Iowa State University Integrated Crop Management News (online newsletter) - 26 May 2009. Tylka, G. 2009. SCN females are now apparent on soybean roots. Iowa State University Integrated Crop Management News (online newsletter) -10 July 2009. Tylka, G. 2009. It is time to sample for corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) -13 July 2009. Tylka, G. 2009. Check SCN resistant soybean roots for SCN females. Iowa State University Integrated Crop Management News (online newsletter) - 31 July 2009. Tylka, G. 2009. Common corn nematode characteristics. Iowa State University Integrated Crop Management News (online newsletter) - 3 August 2009. Tylka, G.L. 2009. Fall is great time to sample for SCN, but not corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) - 6 October 2009. Tylka, G. 2009. Corn nematodes and soybean cyst nematode: basic facts and prospects for 2010. Proceedings of the 2009 Iowa State University Integrated Crop Management Conference, pp. 91-95. [2011] Journal Articles Bao, Y., Neher, D.A., and Chen, S. 2010. Effect of soil disturbance and biocides on nematode communities and extracellular enzyme activity in soybean cyst nematode suppressive soil. Nematology. 13:687-699. Bao, Y., and Neher, D.A. 2011. Survey of lesion and northern root-knot nematodes associated with vegetables in Vermont. Nematropica 41: 98-106. Bernard, E.C., Handoo, Z.A., Powers, T.O., Donald, P.A., and Heinz, R.D. 2010. Vittatidera zeaphila (Nematoda:Heteroderidae), a new genus and species of cyst nematode parasitic on corn (Zea mays). Journal of Nematology 42:139-150. Faghihi, J, P. A. Donald, G. Noel, T. Welacky, and V. R. Ferris. 2010. Soybean resistance to field populations of Heterodera glycines in selected geographic areas. Plant Health Progress. doi:10.1094/PHP-2010-0426-01-RS. Kim, M., D. L. Hyten, T.L. Niblack, and B. W. Diers. 2011. Stacking resistance alleles from wild and domestic soybean sources improves soybean cyst nematode resistance. Crop Science 51:934-943. Mekete, T., K. Reynolds, H. D. Lopez-Nicora, M. E. Gray, and T. L. Niblack. 2010. Plant-parasitic nematodes, potential pathogens of Miscanthus x giganteus and Panicum virgatum used for biofuels. Plant Disease 95:413-418. Mekete, T., K. Reynolds, H. D. Lopez-Nicora, M. E. Gray, and T. L. Niblack. 2010. Distribution and diversity of root-lesion nematode (Pratylenchus spp.) associated with Miscanthus x giganteus and Panicum virgatum used for biofuels and species identification in a multiplex polymerase chain reaction. Nematology DOI:10.1163/ 138855410X538153. Melakeberhan, H., A. Kravchenko, J. Dahl, and D. Warncke (2010). Effects of soil types and Meloidogyne hapla on the multi-purpose uses of arugula (Eruca sativa). Nematology, 12: 115-120. Melakeberhan, H. (2010). Assessing cross-disciplinary efficiency of soil amendments for agro-biologically, economically, and ecologically integrated soil health management. Journal of Nematology, 42: 73-77. Melito, S., Heuberger, A. L., Cook, D., Diers, B. W., MacGuidwin, A. E., and Bent, A. F. 2010. A nematode demographics assay in transgenic roots reveals no significant impacts of the Rhg1 locus LRR-Kinase on soybean cyst nematode resistance. BMC Plant Biology 2010, 10:104 http://www.biomedcentral.com/1471-2229/10/104 Mennan, S. and H. Melakeberhan (2010). Effect of biosolid amendment on populations of Meloidogyne hapla and soils with different textures and pHs. Bioresource Technology, 101:7169-7175. Pedersen, P., G. L. Tylka, A, Mallarino, A. E. MacGuidwin, N. C. Koval, and C. R. Grau. 2010. Correlation between soil pH, Heterodera glycines population densities, and soybean yield. Crop Science 50:1458-1464. Rotundo, J.L., G.L. Tylka, and P. Pedersen. 2010. Source of resistance affects soybean yield, yield components, and biomass accumulation in Heterodera glycines-infested fields. Crop Science 50:2565-2574. Schapaugh Jr., W.T., T. Todd, J. Reese, J. Diaz-Montano, J. Meng, and C.M. Smith. 2010. Registration of K1639-2 soybean germplasm resistant to soybean cyst nematode and soybean aphid. Journal of Plant Registrations 4:67-69. Schroeder, N. E., and MacGuidwin, A. E. 2010. Behavioral quiescence reduces the penetration and toxicity of exogenous compounds in second-stage juveniles of Heterodera glycines. Nematology, 12:277-287. Schroeder, N. E., and MacGuidwin, A. E. 2010. Mortality and behavior in Heterodera glycines juveniles following exposure to isothiocyanate compounds. J. Nematology 42:194-200. Abstracts Arias, M.M.D., G. Tylka, L. Leandro, and G. Munkvold. 2010. Effects of soybean cyst nematode infestation and resistance on Fusarium root rot on soybeans. Phytopathology 100 S30. Donald, P.A., and Tyler, D.D. 2010. Effects of tillage on the interaction of soybean rhizosphere organisms. Journal of Nematology (in press). Donald, P.A., Allen, P.B., Sistani, K., Tyler, D.D., and Tewolde, H. 2010. Effect of poultry litter on Heterodera glycines reproduction. Phytopathology 100:S30. Faghihi, Jamal, R. A. Vierling and V. R. Ferris. 2010. Effect of fall annual rye grass seeding on soybean cyst nematode. Journal of Nematology (in press). Lopez-Nicora, H. D., J.P. Craig, and T.L. Niblack. 2010. Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction. Abstracts of the Annual Meeting of the Society of Nematologists: 76. Journal of Nematology (in press). Lopez-Nicora, H. D., T. Mekete, K. Reynolds, M. E. Gray, and T.L. Niblack. 2010. Distribution and diversity of Pratylenchus spp. associated with biofuel crops and species identification in a multiplex PCR assay. Phytopathology 100:S74. MacGuidwin, A. E. 2010. Population dynamics of Pratylenchus penetrans on corn and the relationship of nematode population densities and corn yield. Society of Nematologists, Boise, Idaho. Journal of Nematology (in press). Mekete, Tesfamariam, Kimberly Reynolds, Horacio D. Lopez-Nicora, Michael E. Gray, and Terry L. Niblack. 2010. Development of species specific primers for molecular diagnostics of plant-parasitic nematodes associated with Miscanthus x giganteus and Panicum virgatum used for biofuels. Journal of Nematology (in press). Melakeberhan, H. (2010). Are we overlooking adaptation and interaction effects when managing nematode parasitic variability? 30th International Symposium of the European Society of Nematologists. 25. Nishanthan, T.and Neher, D.A. Ecology of nematode suppressive soils in Midwest soybean-cropping systems, 2011. Soil Ecology Society, Kelowna, British Columbia, Canada, 24-27 May 2011. Tylka, G.L. 2010. Maximizing effectiveness of extension education efforts. Proceedings of the 49th annual meeting of the Society of Nematologists, p. 117-118. Welacky, T.W., J. Faghihi, P. Donald, G. Noel, and V. Ferris. 2010. Soybean resistance to field populations of Heterodera glycines in selected geographic areas. 30th International Symposium of the European Society of Nematologists Proceedings, p.139. Research Reports Allen, F.L., Johnson, R., Williams, R.C., Jr, McClure, A.T., Newman, M., Donald, P. Soybean Variety Performance Tests in Tennessee. 2010. http://varietytrials.tennessee.edu/pdffiles/2010trialdata/soybean_FINALrpt.pdf. Research Report 11-03. University of Tennessee Institute of Agriculture (Technical Bulletin). Andrews, B., SCN Management Update . 2010, Top Crop Manager, v36, no. 17. Cary, T. R. and Diers, B. 2010 Northern Regional Soybean Cyst Nematode Test report, Group I-IV. Urbana, IL : University of Illinois, Dept. of Crop Sciences. Esker, P., A. MacGuidwin, and R. Proost. 2010. Nematodes: The overlooked yield robbers of corn and soybean. UW Extension. Giesler, L. et al. 2010. Soybean Cyst Nematode Management : Understanding How Management Actions Influence Nematode Populations. http://www.planthealth.info/ LeBoeuf, Ontario Research & Development Program (ORDP), Project # ORD-008, Monitoring for early warning of new Sugarbeet Pests in Ontario. 2010 Interim Report. Niblack, T.L. 2010. SCN resistance ratings, in the Variety Information Program for Soybeans (VIPS) at www.vipsoybeans.org. Schmidt, M. Schmidt, C. et al. 2010. The Sudden Death Syndrome Regional Tests, Annual Report. North Central Soybean Research Program Report. Southern Illinois University of Carbondale, Carbondale, Illinois. Todd, T.C. and W.T. Schapaugh. Female indices and SDS ratings for the 2010 Kansas Soybean Performance Test entries. http://www.agronomy.ksu.edu/extension/~/doc3502.ashx Welacky, T.W. Annual Reports - Ontario Soybean Variety Trials. Ontario Oil and Protein Seed Crop Committee. 12p. Extension Publications Diaz Arias, M., G.L. Tylka, A. Robertson, L. Leandro, and G. Munkvold. 2010. Fusarium root rot of soybean - occurrence, impact, and relationship with soybean cyst nematode. Proceedings of the 2010 Iowa State University Integrated Crop Management Conference, pp. 115-123. Mueller, D., A. Robertson, A. Sisson, and G. Tylka. 2010. Soybean diseases. Iowa State University Extension Publication CSI 0004, 36 pp. Tylka, G.L. and M. P. Mullaney. 2010. Soybean cyst nematode-resistant soybeans for Iowa. Iowa State University Extension Publication PM 1649, 27 pp. Tylka, G.L., G.D. Gebhart, and C.C. Marett. 2010. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa - 2009. Iowa State University Extension, publication IPM 52. Tylka, G.L., G.D. Gebhart, C.C. Marett, M.P. Mullaney, and S.N. Wiggs. 2010. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa - 2010. Iowa State University Extension, publication IPM 52. Conference proceedings Tylka, G. 2010. Corn nematodes: what will 2010 hold for us? Proceedings of the Iowa State University Crop Advantage Series, page 13. Tylka, G. 2010. Nematodes that feed on corn - what to make of it all. Proceedings of the 2010 Iowa State University Integrated Crop Management Conference, pp. 97-101. Tylka, G. 2010. SCN: what will 2010 hold for us? Proceedings of the Iowa State University Crop Advantage Series, page 14. Tylka, G. 2010. The increase in SCN reproduction on resistant soybean varieties - what does it mean? Proceedings of the 2010 Iowa State University Integrated Crop Management Conference, pp. 103-105.

Publications Attachment:

Attachment

Impact Statements

Information from this project, disseminated in reports, presentations, and web sites, helped the soybean industry assess the impact of SCN on soybean production and the importance of planting SCN resistant varieties.
Characterization of virulence for field populations of SCN across the region provided insight to producers and researchers about variability among geographic isolates and the importance of integrating different management tactics for SCN control.
Data on novel approaches as well as established practices such as tillage increased the ability of producers to develop a management plan to maximize yield and minimize reproduction and build-up of SCN.
Adaptability of SCN to different sources of resistance highlighted the need for continued field scouting by producers.
Producers are using additional management tools for field populations of SCN across the NC region. We have provided these by demonstrating variability among geographic isolates and communicating to growers the importance of integrating different management tactics for SCN control. Working together, our group has provided a more complete picture of SCN variability and virulence in the region. This could not have happened if the group had been working independently.
Soybean breeders have objective and independent evaluation of the impact of SCN on commercially available soybean cultivars. This information serves as a guide for future breeding strategies and was obtained through interdisciplinary cooperation with agronomists and others.
The research community has a more refined understanding of conditions favorable for establishment of SCN. Favorable economic return for soybean promotes expansion of production into non-traditional soybean production areas which, in turn, favors expansion of the known distribution of SCN. Understanding of complex plant rhizosphere interactions of SCN with other nematodes, bacteria, fungi and soil microarthropods will lead to a better conceptualization of conditions which favor establishment and build up of SCN in production fields.

Date of Annual Report: 05/01/2008

Report Information

Annual Meeting Dates: 12/17/2006 - 03/05/2008
Period the Report Covers: 10/01/2006 - 09/01/2007

Participants

Avendano, Felicitas, Iowa State University, avendano@iastate.edu;
Bird, George, Michigan State University, birdg@msu.edu;
Chen, Senyu, University of Minnesota, chenx099@umn.edu;
Donald, Pat, USDA, pdonald@ars.usda.gov;
Faghihi, Jamal,Purdue University, jamal@purdue.edu;
Ferris, Virginia, Purdue University, vferris@purdue.edu;
Giesler, Loren, University of Nebraska, lgiesler1@unl.edu;
MacGuidwin, Ann, University of Wisconsin, aem@plantpath.wisc.edu;
Neher, Deb, University of Vermont, deborah.neher@uvm.edu;
Nelson, Berlin, North Dakota State University, Berlin.Nelson@ndsu.nodak.edu;
Niblack, Terry, University of Illinois, tniblack@uiuc.edu;
Powers, Tom, University of Nebraska, tpowers@unl.edu;
Robbins, Robert, University of Arkansas, rrobbin@uark.edu;
Tenuta, Albert, Agriculture Canida, albert.tenuta@ontario.ca;
Tylka, Greg, Iowa State University, gltylka@iastate.edu;
Sweets, Laura, University of Missouri;
Welacky, Tom, Agriculture Canida, welackyt@agr.gc.ca;

Brief Summary of Minutes

Greg Tylka called the meeting to order and welcomed participants at 8:00 a.m. on March 5th, 2008 in Tampa, Florida. The meeting was held one day before the 4th National Soybean Cyst Nematode Conference sponsored, in part, by NC 1035. The meeting, convened to report on activities during 2007, was delayed beyond the scheduled December date to coincide with the SCN conference. State reports were presented with the information organized by project objectives: 1.) Develop, evaluate, improve, and integrate management techniques for SCN in the NCR to increase grower profitability, 2.) Determine and apply the concepts of invasion biology as revealed by SCN epidemiology, 3.) Develop decision-support data bases for management of SCN and other regionally important nematodes. Relating to objective one was discussion about the availability and efficacy of soybean varieties to SCN, SCN resistance genes and the difference among cultivars that share the same source of resistance, the range of virulence phenotypes of SCN for development of resistant varieties, methodology appropriate for characterizing SCN populations, tillage and the impact of soil disturbance on suppression of SCN by fungal antagonists, the interaction of SCN with other pests and pathogens, and soil amendments including manure and cover crops for SCN management. Discussion for objective two focused on surveys for SCN conducted during the past year and the methodology for tier 2 evaluation of soils for conduvciveness to SCN. Progress of SOPs for screening resistant lines of soybean to SCN and an index of commercial varieties resistant to SCN were discussed as pertinant to objective three.

Accomplishments

Short-term outcomes: Improved use of soybean varieties resistant to the soybean cyst nematode (SCN) resulting in higher yields and decreased population densities of SCN. Outputs: Publications as reported in the "Publications" section Activities: Objective One: - Virulence profiles for SCN populations were conducted in Illinois, Minnesota, Ontario, and Wisconsin. - Host resistance for managing SCN was evaluated in Ontario, Kansas, Tennessee, Michigan, Minnesota, Illinois, and Iowa. - Selection pressure imposed by continuous planting of SCN-resistant varieties was studied in Arkansas, Kansas, and Wisconsin. - The impact of soil disturbance and tillage on SCN was determined in Indiana, Minnesota, and Vermont. - Soil amendments for the management of SCN were evaluated in Ontario and Minnesota. - The interaction of SCN with other pests and pathogens was studied in Iowa, Minnesota, and Wisconsin. Objective Two: - Surveys for SCN to identify candidate fields for Tier II activities were conducted in Illinois, Michigan, Minnesota, Nebraska, North Dakota, Ontario, and Wisconsin. Objective Three: - An SOP for screening resistant lines to SCN was developed. - Lists of SCN-resistant varieties available commercially were compiled by Iowa and Tennessee. Milestones: Developed standard format for reporting data relating to objective one.

Publications

Avendano, F. M. E. O'Neal, and G. L. Tylka. 2007. Soybean cyst nematode and soybean aphid interactions on soybean. Journal of Nematology 39:85. Bernard, E. C., E. C., Donald, P. A., Handoo, Z. A. Heinz, R. D., and Powers, T. O. 2007. Characterization of a new species of cyst nematode parasitizing corn. Journal of Nematology 39:74. Bird, G. W. 2007. A Global Perspective of Integrated Nematode Management Innovation. Phytopathology 97:S148. Bird, G. W. 2007. Nematode community structure of natural, non-managed and managed ecosystems. Journal of Nematology 39:89. Chen, S. Y. 2007. Suppression of Heterodera glycines in soils from fields with long-term soybean monoculture. Biocontrol Science and Technology 17:125-134. Chen, S. Y. 2007. Tillage and crop sequence effects on Heterodera glycines and soybean yields. Agronomy Journal 98:897-907. Chen, S. Y., J. E. Kurle, S. R. Stetina, D. R. Miller, G. A. Nelson, L. D. Klossner, and N. C. Hansen. 2007. Interactions between rion-deficiency shlorosis and soybean cyst nematode in Minnesota soybean fields. Plant and Soil 299:131-139. Chen, S. and Liu, S. F. 2007. Effects of tillage and crop sequence on parasitism of Heterodera glycines juveniles by Hirsutella spp. And on juvenile population density. Nematropica 37:93-106. Chen, S., C. C. Sheaffer, D. L. Wyse, P. Nickel, H. Kandel, and C. M. Fernholz. 2007. Distribution and frequency of Heterodera glycines and other plant-parasitic nematodes in the organic-farming fields in Minnesota. Journal of Nematology 39:99. Creech, J. Earl, J. S. Webb, B. G. Young, J. P. Bond, S. K. Harrison, V. R. Ferris, J. Faghihi, A. Westphal, and W. G. Johnson. 2007. Development of soybean cyst nematode (Heterodera glycines) on Henbit (Lamiium amplexicaule) and purple deadnettle (L. purpureum) 2007. Weed Technology 21:1064-1070. Creech, J. Earl, A. Westphal, V. R. Ferris, J. Faghihi, T. J. Vyn. J. B. Santini, and W. O. Johnson. 2007. Influence of winter annual weed management and crop rotation on soybean cyst nematode (Heterodera glycines) and winter annual weeds. Weed Science 56:103-111. Davenport, J., G. Bird and F. Warner. 2007. Dynamics of HG Types Associated with Commercial soybean Cultivars in Michigan. J. Nematol. 39:90. Donald, P. A., T. Kilen, and A. Mengitsu. 2007. Registration of soybean germplasm line D99-2018 resistant to Phytophthora rot (Rps6) and soybean cyst nematode races 3 (Hg Type 0) and 14 (Hg Type 1.3.6.7). Crop Science 47:451-452. Donald, P. A., Hayes, R., and Walker, E. 2007. Potential for soybean cyst nematode reproduction on winter weeds and cover crops in Tennessee. Online. Plant Health Progress do8i:1094/PHP-2007-0226-01-RS. Donald, P. A., and Tyler, D. D. 2007. Long- and short-term tillage effects on Heterodera glycines reproduction. Journal of Nematology 39:72. D., J. Andresen, G. Bird, J. Flore, L. Gut, P. McManus, J. Nugent, R. Isaacs, A. Schilder, M. Whalon, R. Sirrine, J. Sanchez. 2007. Tart Cherry Systems, pp. 74-101 (in) Ecologically Based Farming Systems, Deming, S., L. Johnson, D. Lehnert, D. Mutch, L. Probyn, K. Renner, J. Smeenk, S. Thalmann and L. Worthington (eds). Michigan State University Extension Bulletin E-2983. Lansing, 140 pp. Faghihi, J., S. Conley, and V. R. Ferris. 2007. Field reaction of selected SCN resistant soybean germ plasm toward SDS. Journal of Nematology 39 (1):76. Gao, X., A. L. Colgrove, K. N. Lambert, and T. L. Niblack. 2007. A molecular assay for reliable quantification of soybean cyst nematode in plants. Journal of Nematology 39:94. Gavassoni, W. L., G. L. Tylka, and G. P. Munkvold. 2007. Relationships among tillage practices, dissemination, and spatial patterns of Heterodera glycines and soybean yield. Plant Disease 91:973-978. Masler, E. P., P. A. Donald, S. Sardanelli. 2008. Stability of Heterodera glycines (Tylenchida:Heteroderidae) juvenile hatching from eggs obtained from different sources of soybean, Glycine max. Nematology. 10:271-278. Mebrahtu, T., Devine, T. E., Donald, P. A., and Abney, T. S. 2007. Registration of Owens vegetable soybean. Journal of Plant Registration 1:95-96. Melakeberhan, H., Mennan, S., Chen, S., Darby, B., and Dudek, T. 2007. Integrated approaches to understanding and managing Meloidogyne hapla populations parasitic variability. Crop Protection 26:894-902. Mengistu, A., Kilen, T. C., and Donald, P. A. 2007. Registration of D95-5048 soybean germplasm line resistant to Phytophthora rots and soybean cyst nematode races 3 and 14. Crop Science 47:452. Mennan, S., Chen, S. Y., and Melakeberhan, H. 2007. Effects of Hirsutella minnesotensis and N-Viro soil on populations of Meloidogyne hapla. Biocontrol Science and Technology 17:233-246. Mock, V. A., J. E. Creech, Bill Johnson, Jamal Faghihi, V. R. Ferris, A. Westphal, K. Bradley. 2007. Winter Annual Weeds and Soybean Cyst Nematode Management. Purdue Extension Publication. Niblack, T. L., Colgrove, K., and Bond, J. P. 2008. Shift in virulence of soybean cyst nematode is associated with use of resistance from PI 88788. Online. Plant Health Progress doi:10.1094/PHP-2008-0118-01-RS. Pedersen, P. and G. Tylka. 2007. Soybean cyst nematode and nematicide affect growth patterns of various soybean varieties in Iowa. Journal of Nematology 39:98. Schroeder, N. E., and A. E. MacGuidwin. 2007. Incorporation of a fluorescent compound by live Heterodera glycines. Journal of Nematology 39:3-49. Snapp, S, D. Smucker, R. Chase, W. Kirk, G. Bird and D. Douches. 2007. Michigan Potato Systems, pp. 60-73 (in) Ecologically Based Farming Systems, Deming, S., L. Johnson, D. Lehnert, D. Mutch, L. Probyn, K. Renner, J. Smeenk, S. Thalmann and L. Worthington (eds). Michigan State University Extension Bulletin E-2983. Lansing, 140 pp. Sun, M., S. Chen, J. E. Kurle, S. Naeve, D. L. Wyse, LO. A. Stahl, G. A. Nelson, and L. D. Klossner. 2007. Effect of rotation crops on vesicular-arbuscular mycorrhizal fungi and iron-deficiency chlorosis of soybean. Phytopathology 97:S113. Tabor, G. M., G. L. Tylka, and C. R. Bronson. 2007. Genotypes A and B of Cadophora gregata differ in ability to colonize susceptible soybean. Plant Disease 91:574-580. Tabor, G. M., G. L. Tylka, and C. R. Bronson. 2007. Genotype B of Cadophora gregata reduces quantity and quality of soybean yield. Phytopathology 97:S113. Xiang, M. C., Liu, X. Z., Xiao, Q. M., and Chen, S. Y. 2007. Nutritional requirements of the nematophagous fungus Hirsutella minnesotensis. Acta Phytopathologica Sinica 37:151-168. Xiang, M. C., Wang, Z. X., Liu, X. Z., Yang, X. H., Chen, S. Y., and Ziao, Q. M. 2007. Variability of morphology, parasitism, and nucleotide sequences among isolates and species of nematophagous Hirsutella. Biological Control 33:110-119. Xiao, J., J. Zhu, S. Chen, W. Ruan, and C. Miller. 2007. A novel use of anaerobically digested liquid swine manure to potentially control soybean cyst nematode. Journal of Environmental Science and Health Part C- - Pesticides Food Contaminants and Agricultural Wastes 42:749-757. Xiao, J., J. Zhu, S. Chen, W. Ruan, and C. Miller. 2007. Control of the soybean cyst nematode using anaerobically digested liquid swine manure. Journal of Nematology 39:73. Zasada, I., F. Avendano, S. Koenning, Y. Li, T. Logan, H. Melakeberhan, and G. Tylka. 2008. Potential of an alkaline-stabilized biosolid to manage nematodes: case studies on soybean cyst and root-knot nematodes. Plant Disease 92:4-13.

Impact Statements

Information from this project, disseminated in reports, presentations, and web sites, helped the soybean industry assess the impact of SCN on soybean production and the importance of planting SCN resistant varieties.
Characterization of virulence for field populations of SCN across the region provided insight to producers and researchers about variability among geographic isolates and the importance of integrating different management tactics for SCN control.
Data on novel approaches as well as established practices such as tillage increased the ability of producers to develop a management plan to maximize yield and minimize reproduction and build-up of SCN.

Date of Annual Report: 04/23/2009

Report Information

Annual Meeting Dates: 12/10/2008 - 12/11/2008
Period the Report Covers: 10/01/2007 - 09/01/2008

Participants

Bird, George, Michigan State University, birdg@msu.edu; Chen, Senyu, University of Minnesota, chenx099@umn.edu; Donald, Pat, USDA, pat.donald@ars.usda.gov; Faghihi, Jamal, Purdue University, jamal@purdue.edu; Ferris, Virginia, Purdue University, vferris@purdue.edu; MacGuidwin, An, University of Wisconsin, aem@plantpath.wisc.edu; Melakeberhan, Haddish, Michigan State University, melakebe@msu.edu; Nelson, Niblack, Terry, University of Illinois, tniblack@uiuc.edu; Tylka, Greg, Iowa State University, gltylka@iastate.edu; Welacky, Tom, Agriculture Canada, welackyt@agr.gc.ca

Absent: Tim Todd (Kansas), Steve Pueppke (Administrative advisor), Tom Powers (Nebraska), Deb Neher (Vermont)

Guest: Berlin Nelson

Brief Summary of Minutes

An MacGuidwin called the meeting to order and welcomed participants at 8:30 a.m. on December 10, 2008 in Chicago, IL. This meeting was held in conjunction with the American Seed Trade Association and the NCDC 214 (Corn Pathology Committee) as well as other committees. This meeting was held within nine months of the last meeting to facilitate interactions with the Corn Pathology Committee (NCDC214) which was interested in discussion of results and future plans with NC1035 members participating in the corn nematode surveys sponsored by Syngenta Corporation. State reports were presented with the information organized by project objectives:
1.) Develop, evaluation, improve, and integrate management techniques for SCN in the NCR to increase grower profitability. Most committee members participated in the regional variety tests managed by the National Soybean Research Laboratory, Urbana-Champagne, IL either through field trials or greenhouse testing of the 334 soybean lines. These data were published in the fall and spring prior to the next growing season. Development of a common web link for all variety performance reports was initiated. Additional research involved collaboration on evaluation of soybean lines for each state. A collaborative study funded by North Central Soybean Research Program on the assessment of SCN population HG Types specifically in relation to reproduction on PI 88788 ended in 2008 and results were compared to studies in other states conducting similar studies. Research on interactions of SCN with other pests was included.
2.) Determine and apply the concepts of invasion biology as revealed by SCN epidemiology. Discussion for objective two focused on clarification of procedures to complete this objective. Several states initiated the Tier 1 and 2 studies characterizing SCN invasive biology. In February 2008 a proposal was resubmitted to NRI- Nematodes and Arthropods, Organismal Biology entitled, Ecology of Nematode-suppressive Soils in Midwest Soybean-cropping Systems. The 4-year project was funded in full to address three objectives: 1) Quantify the effects of rotation with non-host crop and resistant soybean, and tillage on soil suppressiveness to Heterodera glycines; 2) Identify functional groups and key species of parasitic, antagonistic or predaceous fungi and bacteria involved in suppression of Heterodera glycines and their response to cropping systems that vary in crop rotation and tillage; 3) Compare community composition and food web structure of predaceous, fungivorous and bacterivorous nematodes associated with suppression of Heterodera glycines and their response to cropping systems that vary in crop rotation and tillage. 3.) Develop decision-support data bases for management of SCN and other regionally important nematodes. Standard operating procedures for screening SCN resistant lines (Standard Cyst Evaluation 2008 -SEC08) was developed in cooperation with industry and accepted at the 4th National SCN Conference by industry, public and private researchers. . A manuscript is in draft stage. A NSF funded study using complete nematode community structure analysis has been completed for five types of ecosystems. A study of plant parasitic nematodes found in Vermont vegetable production fields was conducted and Pratylenchus were found in highest levels.

Accomplishments

Short-term outcomes: More unbiased information on SCN resistant varieties and soybean yield in relation to SCN population density is available. Outputs: Publications are reported in the Publications section. Activities: Objective One: - Virulence profiles for SCN populations were conducted in Illinois, Indiana, Minnesota, Ontario, Tennessee, and Wisconsin. Host resistance for managing SCN was evaluated in Ontario, Michigan, Minnesota, Illinois and Tennessee. The impact of soil disturbance and tillage on SCN was measured in Indiana, Minnesota, and Tennessee. The interaction of SCN with other pests and pathogens was studies in Illinois, Iowa, Minnesota, Ontario, and Wisconsin. Alternative host studies were conducted in Indiana and Ontario. Community structure studies were conducted in Michigan, Vermont, and Tennessee. Objective Two: Field results from candidate fields for Tier II activities were compared. Funding for this objective was secured. Objective Three: An SOP for screening resistant lines to SCN was developed (SEC08). Lists of SCN-resistant varieties available commercially were compiled by Illinois, Iowa, Ontario, and Tennessee and provided to soybean producers. Milestones: Data on corn nematodes was presented to corn pathologists to further cooperation between the two groups.

Publications

Publications Attachment:

Attachment

Impact Statements

Characterization of virulence for field populations of SCN across the region provided insight to producers and researchers about variability among geographic isolates and the importance of integrating different management tactics for SCN control.
Information from this project, disseminated in reports, presentations, and web sites, helped the soybean industry assess the impact of SCN on soybean production and the importance of planting SCN resistant varieties. Adaptability of SCN to different sources of resistance highlighted the need for continued field scouting by producers.

Date of Annual Report: 09/25/2009

Report Information

Annual Meeting Dates: 07/16/2009 - 07/17/2009
Period the Report Covers: 10/01/2008 - 07/01/2009

Participants

" Chen, Senyu, University of Minnesota, chenx099@umn.edu

" Donald, Pat, USDA, pat.donald@ars.usda.gov

" Ferris, Virginia, Purdue University, vferris@purdue.edu

" MacGuidwin, An, University of Wisconsin, aem@plantpath.wisc.edu

" Melakeberhan, Haddish, Michigan State University, melakebe@msu.edu

" Neher, Deborah, University of Vermont, dneher@uvm.edu

" Powers, Tom, University of Nebraska, tpowers@unlnotes.unl.edu

" Pueppke, Steve, Administrative Adviser, Pueppke@msu.edu

" Robbins, Robert, University of Arkansas, rrobbin@uark.edu

" Welacky, Tom, Agriculture & Agri-Food Canada, tom.welackyt@agr.gc.ca

" Guests: Berlin Nelson, North Dakota State University, Berlin.Nelson@ndsu.edu George Bird, Michigan State University, birdg@msu.edu , Bao Yong, Tharshani Nishanthan, Scott Costa, University of Vermont

" Absent: Tim Todd (Kansas), Jamal Faghihi, (Indiana), Terry Niblack, (Illinois), Greg Tylka, (Iowa)

Brief Summary of Minutes

Summary of Minutes of Annual Meeting:
Pat Donald called the meeting to order and welcomed participants at 8:30 am at the University Heights Housing meeting room, University of Vermont . This meeting was held in conjunction with the joint meeting of the 48th Society of Nematologists annual meeting and the Soil Ecology Society, 12th biennial meeting.
The primary purpose of the meeting to review the current status and participation in the NC1035 Project Objectives and to report recent research results since the last meeting held in March 2009.

Planning session and review of project Objectives

Objective 1: To develop, evaluate, improve and integrate management techniques for soybean cyst nematode in the NC region to increase grower profitability. Discussion of the objective indicated that there was a general consensus that the majority of the committee are generating results for all of the sub objectives and that the members were on track in meeting the goals for this objective. These data were published in the fall and spring prior to the next growing season. Research on interactions of SCN with other pests were included. Exploratory work is being conducted by several members of the group on the nematicide seed treatments that are being explored by several private companies.

Objective 2: To apply the concepts of invasion biology to H. glycines epidemiology. The Tier 1, 2 and 3 were outlined and a review of committee member participation indicated that the majority had completed Tier 1 protocol (Initial assessment of habitats for occurrence of H. glycines). Tier 2 protocol (Habitat conduciveness evaluation) was clarified by project writers and it was noted that only a few participants had completed this level. The need to identify suppressive soils and levels of conducive field populations was emphasized. Members were encouraged to complete this section to provide results for progression to Tier 3 (Associated soil quality assessment) and Tier 4 Protocol (Invasive biology model validation). Considerable discussion on the feasibility of completing Tier 3 and 4 protocols in the remaining project time frame and the need for research funding was not resolved.

Objective 3: To develop a decision-support database for management of H. glycines and other regionally important nematodes. A limited discussion was held.

Accomplishments

Accomplishments: Short-term outcomes: Data on H. glycines virulence phenotypes across the region were shared. Additional unbiased information on H. glycines resistant varieties and soybean yield in relation to SCN population density is available. Outputs: Outputs: Publications are reported in the Publications section. Michigan presented a reference handbook for growers, Soybean Cyst Nematode (SCN) Management by D. Brown-Rytlewski, G. Bird, F. Warner and K. Barnett, to the committee for discussion. The Michigan publication was submitted as part of the NC1035 project objective to develop decision-support data bases for management of SCN for the grower community Activities: Objective One: - Virulence profiles for H. glycines populations were conducted in Indiana, Minnesota, Ontario, and Tennessee. Host resistance for managing H. glycines was evaluated in Ontario, Michigan, and Tennessee. Funding for this activity was obtained from the Tennessee Soybean Promotion Board and Ontario Soybean Board. The impact of soil disturbance and tillage on H. glycines was measured in Tennessee. The interaction of H. glycines with other pests and pathogens was studied in Indiana. Alternative host studies were conducted in Indiana. Community structure studies were conducted in Michigan, Vermont, and Tennessee. Objective Two: Field results from candidate fields for Tier II activities were compared. Funding for a related objective was secured for Minnesota and Vermont. Objective Three: A SOP for screening resistant lines to H. glycines was published (SEC08). Lists of H. glycines-resistant varieties available commercially were compiled by Ontario, and Tennessee and provided to soybean producers. Milestones: Documentation of virulence phenotype variability across the north central region is vital information for public and private soybean breeders.

Publications

Scientific: Donald, P.A., Patananan, A., Cerna, C.S., Simmons, T., Castro, N., Wilgar, J.L., and Goheen, S.C. 2008. Protein SDS-PAGE characterization and comparison of soybean cyst nematodes. Nematropica 38: 137-144. Donald, P.A., Tyler, D.D., and Boykin, D. 2009. Short and long-term tillage effects on Heterodera glycines reproduction in soybean monoculture in west Tennessee. Soil and Tillage Research 104:126-133. Melakeberhan. H., S. Mennan, M. Ngouajio, and T. Dudek (2008). Effect of Meloidogyne hapla on multi-purpose use oilseed radish (Raphanus sativus). Nematology 10: 375-379. Melakeberhan, H. (2008). Nutrient use efficiency and precision management of nematodes-Concepts and possibilities. Nematologia Brasileira 32:1-12. Niblack, T.L., Tylka, G.L., Arelli, P., Bond, J., Diers, B., Donald, P., Faghihi, J., Ferris, V.R., Gallo, K., Heinz, R.D., Lopez-Nicora, H., Von Qualen, R., Welacky, T., and Wilcox, J.A. 2009. A standard greenhouse method for assessing soybean cyst nematode resistance in soybean:SCE08 (Standardized Cyst Evaluation 2008). Online. Plant Health Progress doi:10.1094/PHP-2009-0513-01-RV. Poromarto, S. H, and Nelson, B. D. 2009. Reproduction of soybean cyst nematode on dry bean cultivars adapted to North Dakota and northern Minnesota. Plant Disease 93: 507-511. Poromarto, S., and Nelson B. D. 2009. Crops hosts of soybean cyst nematode in the northern Great Plains. Phytopathology 99: S103-104. Poromarto, S. and Nelson, B. 2008. Effects of soybean cyst nematode on growth of dry bean. Phytopathology 98:S126. Melakeberhan, H., M. F. Avendano (2008). Spatio-temporal consideration of soil conditions and site-specific management of nematodes. Precision Agriculture 9: 341-354. Zasada, I., M. F. Avendano, Li, Y. C., T. Logan, H. Melakeberhan., S. R. Koenning, G. L. Tylka (2008). Potential of alkaline-stabilized biosolid to manage nematodes: Case studies on soybean cyst and root-knot nematodes. Plant Disease 92:4-13. Technical: Brown-Rytlewski, D., G. Bird, F. Warner and K. Barnett, 2009. Soybean Cyst Nematode (SCN) Management A field guied for SCN management in Michigan. North Central Soybean Research Program and Michigan Soybean Checkoff. Michigan State University Extension, 27 p. Mock, V. A. J. E. Crech, W. Johnson, J. Faghihi, V. R. Ferris, A Westphal, K. Bradley. 2007. Winter Annual Weeds and Soybean Cyst Nematode Management. (With a guide for identifying known weed hosts). Purdue University Extension WS-36. Abstracts: Melakeberhan. H. (2008). Roles for nematology in understanding renewable energy needs and balancing food and socio economic interests. 5th International Congress of Nematology, Brisbane Australia. 117 Melakeberhan. H., A. Kravchenko, and M. Ngouajio (2008). Strategies for enhancing brassicas multipurpose attributes in managing nematode parasitism complexities. 3rd International Biofumigation Symposium, Canberra, Australia. 37. Welacky, T.W., T. Anderson, C. Fox, D. Hunt, and R. Michellutti. 2009. Effect of Riparian Hedgrows on beneficial organisms and economic pests in Ontario. Society of Nemotologists 48th Annual Meeting, Burlington Vermont. p 49.

Impact Statements

Impact Statements: 1. Characterization of virulence for field populations of H. glycines across the region provided insight to producers and researchers about variability among geographic isolates and the importance of integrating different management tactics for H. glycines management. 2. Information from this project, disseminated in reports, presentations, and web sites, helped the soybean industry assess the impact of H. glycines on soybean production and the importance of planting H. glycines resistant varieties. Adaptability of H. glycines to different sources of resistance highlighted the need for continued field scouting by producers.

Date of Annual Report: 08/30/2010

Report Information

Annual Meeting Dates: 06/29/2010 - 06/30/2010
Period the Report Covers: 07/01/2009 - 06/01/2010

Participants

Chen, Senyu, University of Minnesota, chenx099@umn.edu, (Chair);
Donald, Pat, USDA, pat.donald@ars.usda.gov;
Faghihi, Jamal, Purdue University, jamal@purdue.edu;
Li, Yuhong, Ohio State University, Li.1345@osu.edu, (on behalf of C. Taylor);
MacGuidwin, An, University of Wisconsin, aem@plantpath.wisc.edu;
Melakeberhan, Haddish, Michigan State University, melakebe@msu.edu;
Neher, Deborah, University of Vermont, dneher@uvm.edu;
Niblack, Terry, University of Illinois, tniblack@uiuc.edu;
Robbins, Robert, University of Arkansas, rrobbin@uark.edu;
Todd Tim, Kansas State University, nema@ksu.edu;
Tylka, Gregory, Iowa State University, gltylka@iastate.edu;
Welacky, Tom, Agriculture & Agri-Food Canada, tom.welackyt@agr.gc.ca(recorder);
Guests: Jennifer Riggs, Product Development and Sarah Mikesell, Promotions, Bayer Crop Science;
Absent: Virginia Ferris, Purdue University; Steve Pueppke, Administrative advisor; Berlin Nelson, North Dakota State University; George Bird, Michigan State University; Tom Powers, University of Nebraska.

Brief Summary of Minutes

**PLEASE SEE ATTACHED "COPY OF MINUTES" FILE FOR INDIVIDUAL STATE REPORTS

Summary of Minutes of Annual Meeting:

Senyu Chen called the meeting to order and welcomed participants at 8:30 am at the University of Minnesota Southern Research and Outreach Center meeting room. The following day, Dr. Forest Izuno, the head of the Center, welcomed and provided an outline of the work carried out at the research and outreach center.

The primary purpose of the meeting was to a) discuss development of a potential new North Central project; b) review the current status and participation in the NC1035 Project Objectives; and c) to report recent research results since the last meeting held in July 2009.
Bayer CropScience provided a presentation and discussion of the new corn and soybean seed treatment product VOTiVO following the meetings.

Planning session and review of project Objectives

A) Discuss development of a potential new North Central project

The committee members spent half a day in the morning of June 29 to discuss extensively soybean and corn nematode research requirements across each of the northcentral states and Ontario. The committee drafted a project outline including objectives, milestones, supporting outreach outputs and assignment of timelines and volunteers for preparing a written outline.
Proposed draft of new project main objectives:
Project: Practical Management of Nematodes on Corn, Soybeans and Other Crops of Regional Importance.
Objective 1: Develop, evaluate, improve and integrate management techniques for plant-parasitic nematodes in the North Central Region to increase grower profitability.
Objective 2: Determine the relationships among nematode population characteristics, crop injury and soil health.
Objective 3: Develop tools for technology transfer for management of regionally important nematodes with special reference to soybean cyst nematode and corn parasitic nematodes.

B) Review the current status and participation in the NC1035 Project Objectives

Objective 1: To develop, evaluate, improve and integrate management techniques for soybean cyst nematode in the NC region to increase grower profitability. Discussion of the objective indicated that there was a general consensus that the majority of the committee are generating results for all of the sub objectives and that the members were on track in meeting the goals for this objective. Some of these data were published in the fall and spring prior to the next growing season. Several variety publication reports and web sites were presented in the state reports (note Activities section) and reviewed as outreach sources of information to the soybean producer. Additional research on the assessment of SCN population HG Types and other aspects of virulence was presented by the majority of the committee. There was considerable discussion of future need for research and potential participation in a proposed all state HG type survey.
Biocontrol results from use of chicken waste and swine manure projects indicated some potential effects on SCN eggs (see State reports in Activites section).
Research on interactions of SCN with other pests such as root lesion nematodes were included. Exploratory work is being conducted by several members of the group on the soybean and corn nematicide seed treatments that are being explored by several private companies.

Objective 2: To apply the concepts of invasion biology to H. glycines epidemiology. Results Completion of Tier 1, 2 and 3 were outlined and a review of committee member participation indicated that the majority had completed Tier 1 protocol (Initial assessment of habitats for occurrence of H. glycines).
Tier 2 protocol (Habitat conduciveness evaluation) Report of results from the cooperative project USDA-2009-35302-05261, Ecology of nematode-suppressive soils in Midwest soybean-cropping systems (see report in Activities) was presented. Outcome was to expand participation in a future study of suppressive soils as part of the work on evaluation of SCN habitat and epidemiology. The need to identify suppressive soils and levels of conducive field populations was emphasized.
Committee consensus was that the feasibility of completing Tier 3 and 4 protocols was dependent on external funding. Funding was not obtainable by most members with the exception of the previously mentioned research group. The need for research funding was discussed and not resolved.

Objective 3: To develop a decision-support database for management of H. glycines and other regionally important nematodes.
A list of 716 SCN resistant soybean varieties available to Iowa growers was compiled containing information is available in hard copy or in PDF format on the Iowa State SCN web site.
A limited discussion was held on creating a pocket size information card on how to sample soybean fields on one side and corn fields on the other side for nematodes. A guide for sampling corn nematodes is being developed in Wisconsin.

Minutes Attachment:

Attachment

Accomplishments

Accomplishments: Short-term outcomes: Data on H. glycines virulence phenotypes across the region were shared. Additional unbiased information on H. glycines resistant varieties and soybean yield in relation to SCN population density is available. Activities are described on the attached document listing State Research Activity Reports. Outputs are reported in the State Reports found in the Activities Attachment.

Publications

Journal Articles Delheimer, J. C., T. Niblack, and B. W. Diers. 2009. Comparison of the effects in field tests of the SCN resistance genes from different resistance sources. Crop Science (accepted). Faghihi, Jamal, P. A. Donald, G. Noel, T. W. Welacky, and V. R. Ferris. Soybean Resistance to Field Populations of Heterodera glycines in Selected Geographic Areas. Plant Health Progress. doi:10.1094/PHP-2010-D426-01-RS. Mekete, T., M. E. Gray, and T. L. Niblack. 2009. Distribution, morphological description, and molecular characterization of Xiphenema and Longidorus spp. associated with plants (Miscanthus spp. and Panicum virgatum) used for biofuels. GCB Bioenergy 1:257-266. doi: 10.1111/j.1757-1707.2009.01020.x. Niblack, T.L., G.L. Tylka, P. Arelli, J. Bond, B. Diers, P. Donald, J. Faghihi, V.R. Ferris, K. Gallo, R.D. Heinz, H. Lopez-Nicora, R. Von Qualen, T. Welacky, and J. Wilcox. 2009. A standard greenhouse method for assessing soybean cyst nematode resistance in soybean: SCE08 (Standardized Cyst Evaluation 2008). Online. Plant Health Progress doi:10.1094/PHP-2009-0513-01-RV. Poromarto, S. H., and Nelson, B. D. 2010. Evaluation of northern-grown crops as hosts of soybean cyst nematode. Online. Plant Health Progress doi:10.1094/PHP-2010-0315-02-RS. Rogovsa, N., F.W. Blackmer, and G.L. Tylka. 2009. Soybean yield and soybean cyst nematode densities related to soil pH, soil carbonate concentrations, and alkalinity stress index. Agronomy Journal 101:1019-1026 (also online doi:10.2134/agronj2008.0086x). Studham, M., G.C. MacIntosh, F. Avendaño, D. Soh, and G.L. Tylka. 2009. The soybean resistance gene Rag1 does not protect against soybean cyst and root-knot nematodes. Online. Plant Health Progress doi:10.1094/PHP-2009-0401-01-BR. Zheng, J., Zhang, Y., Li, X., Zhao, L., and Chen, S. 2009. First report of the soybean cyst nematode, Heterodera glycines, on soybean in Zhejiang, Eastern China. Plant Disease 93:319. Abstracts Chen, S., and Zheng, J. 2009. Fast loss of resistance in cultivars with current sources of resistance: A challenge in soybean cyst nematode management. World Soybean Research Conference VIII Proceedings (2009) Online http://www.wsrc2009.cn/en/index.asp. Donald, P.A. and Hewlett, T. Pasteuria nishizawae studies in Tennessee. Journal of Nematology (in press). 2009. Faghihi, J., Donald, P., Welacky, T., Noel, G., and Ferris, V. 2009. Geographic differences in field populations of soybean cyst nematode (SCN). Journal of Nematology (in press). Giesler, L.J., C. Bradley, A. Dorrance, T. Niblack, G. Tylka, D. Jardine, D. Malvick, L. Sweets, S. Markell, L. Osborne, P. Esker, G. Bird, J. Faghihi, and A. Tenuta. 2009. Improving management of soybean cyst nematode through extension demonstration and outreach. Proceedings of the 2009 North Central Division Meeting of the American Phytopathological Society. p. 21. Lopez Nicora, H.D., J. P. Craig, and T. L. Niblack. 2009. A method to assess infection of soybean roots by soybean cyst nematode with quantitative polymerase chain reaction. Phytopathology 99:S76. Lopez-Nicora, H.D., J. Morrison, D. Feltes, and T. L. Niblack. 2009. Distribution and densities of plant-parasitic nematodes on Zea mays in northern Illinois. Journal of Nematology (in press). Mengistu, T. M., and T. Niblack. 2009. Distribution, morphological description and molecular characterization of Pratylenchus spp. associated with biofuel crops. Phytopathology 99:S84. Melakeberhan, H. (2009a). Managing nematode parasitic variability: case studies of soybean cyst nematode and root-knot nematodes. 19th Symposium of the Nematological Society of Southern Africa Proceedings. 25. Melakeberhan, H. (2009b). Application of fertilizer-use efficiency model changing and managing soil conditions in agro-biologically integrated ways. 19th Symposium of Nematological Society of Southern Africa Proceedings. 49. Melakeberhan, H. (2009c). Cross-disciplinary efficiency assessment of soil amendments. Proceeding of the Joint Meeting of the Society of Nematologists and Soil Ecology Society. 146. Melakeberhan, H. (2009d). Fertilizer use efficiency model for managing nematodes and plant, soil and environmental health. SSSA, S-4. CD-Rom. Melakeberhan, H. (2009e). Perspectives for nematology education in the 21st Century: Lessons from the past to make the future better. Proceeding of the Joint Meeting of the Brazilian Society of Nematologists and Society of Tropical American Nematologists. CD-Rom. Poromarto, S. H., and Nelson, B. D. 2010. Effects of soybean cyst nematode on growth of kidney and navy bean. Phytopathology 100:S102 (Abstract) Poromarto, S. H., and Nelson, B. D. 2010. Lack of adaptation toward greater reproduction of soybean cyst nematode on dry bean. Phytopathology 100:S102 (Abstract) Rudolph, K., Bolton, M. D., and Nelson, B. D. 2010. Soybean cyst nematode infects roots of sugar beet. Phytopathology 100:S111 (Abstract) Tylka, G.L., G.D. Gebhart, and C. C. Marett. 2009. Performance of SCN-resistant soybean varieties in fields infested with different soybean cyst nematode HG types. Proceedings of the 2009 North Central Division Meeting of the American Phytopathological Society. pp. 28-29. Zheng, J. W. and Chen, S. Y. 2009. Estimating virulence of soybean cyst nematode field populations in response to use of resistant cultivars. Society of Nematologists 48th Annual Meeting & Soil Ecology Society 12th Biennial Meeting Abstracts:212. (Abstr.). Research Reports: Cary, T. R. and Diers, B.SCN resistance ratings, in the Variety Information Program for Soybeans (VIPS) at www.vipsoybeans.org 2010 Northern Regional Soybean Cyst Nematode Test report, Group I-IV. Urbana, IL : University of Illinois, Dept. of Crop Sciences. Johnson, Allen, F.L., R., Williams, R.C., Thompson, A., Newman, M., Donald, P. 2009. Soybean variety performance tests in Tennessee. http://varietytrials.tennessee.edu/soybean.htm Newman, M. et al.. 2009 Soybean disease ratings and yields 2009 test summaries. University of Tennessee Extension. http://www.utextension.utk.edu/fieldCrops/soybean/diseases/disease_images/2009-Soybean-Report.pdf Welacky, T.W. 2010 Report - Ontario Soybean Variety Trials. Ontario Oil and Protein Seed Crop Committee. 12p. Producer and editor of internet site, http://www.gosoy.ca with graphs, % Protein and % Oil reports and tables of varietal responses to SCN and various diseases in Ontario (3,000 visits annually). Soy Data editor and production co-coordinator of interactive internet web site for seed sponsors and test cooperators to organize entry information for yearly Ontario Soybean Variety Trial plots across 20 locations in Ontario, Quebec and Manitoba. Hager, H. 2009. Breakdown of Soybean Resistance to Soybean Cyst Nematode. Top Crop Manager East, January, 2009, p. 6-7. Extension Publications: Tylka, G.L. 2009. Soybean cyst nematode-resistant soybean varieties for Iowa. Iowa State University Extension Publication PM 1649, 24 pp. Tylka, G.L., G.D. Gebhart, and C.C. Marett. 2009. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa 2008. Iowa State University Extension Publication IPM 52, 26 pp. Tylka, G. 2009. Quick facts about corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) - 28 April 2009. Tylka, G. 2009. Spring sampling not recommended for most corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) - 7 May 2009. Tylka, G. 2009. SCN and Corn Nematode Workshops set for July Iowa State University Integrated Crop Management News (online newsletter) - 26 May 2009. Tylka, G. 2009. SCN females are now apparent on soybean roots. Iowa State University Integrated Crop Management News (online newsletter) -10 July 2009. Tylka, G. 2009. It is time to sample for corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) -13 July 2009. Tylka, G. 2009. Check SCN resistant soybean roots for SCN females. Iowa State University Integrated Crop Management News (online newsletter) - 31 July 2009. Tylka, G. 2009. Common corn nematode characteristics. Iowa State University Integrated Crop Management News (online newsletter) - 3 August 2009. Tylka, G.L. 2009. Fall is great time to sample for SCN, but not corn nematodes. Iowa State University Integrated Crop Management News (online newsletter) - 6 October 2009. Tylka, G. 2009. Corn nematodes and soybean cyst nematode: basic facts and prospects for 2010. Proceedings of the 2009 Iowa State University Integrated Crop Management Conference, pp. 91-95.

Impact Statements

Date of Annual Report: 08/11/2011

Report Information

Annual Meeting Dates: 06/23/2011 - 06/24/2011
Period the Report Covers: 10/01/2009 - 09/01/2010

Participants

Faghihi, Jamal (jfaghihi@purdue.edu) - Purdue University; MacGuidwin, An (aem@plantpath.wisc.edu) - University of Wisconsin; Pueppke, Steve (Pueppke@msu.edu) - Administrative Advisor; Niblack, Terry (tniblack@uiuc.edu) - University of Illinois; Robbins, Robert (rrobin@uark.edu) - University of Arkansas; Todd, Tim (nema@ksu.edu) - Kansas State University; Tylka, Greg (gltylka@iastate.edu) - Iowa State University; Welacky, Tom (tom.welackyt@agr.gc.ca) - Agriculture and Agri-Food Canada;

Absent: ; Chen, Senyu (chenx099@umn.edu) - University of Minnesota; Donald, Pat (pat.donald@ars.usda.gov) - USDA; Ferris, Virginia (vferris@purdue.edu) - Purdue University; Melakeberhan, Hadish (melakebe@msu.edu) - Michigan State University; Neher, Deborah (dneher@uvm.edu) - University of Vermont; Powers, Tom (tpowers@unlnotes.unl.edu) - University of Nebraska

Brief Summary of Minutes

The meeting was convened at the University of Wisconsin Microbial Sciences Building. Tim Todd called the meeting to order at 8:30 on June 23. Following introductions and local arrangements information, participants proceeded with individual state reports. During the afternoon session, Steve Pueppke announced approval of the new project NC 1197. State reports were concluded and the annual Business Meeting was conducted on June 24. Tennessee was chosen as the preliminary site for the 2012 annual meeting.

The primary purpose of the meeting was to report and discuss recent research results obtained since the last meeting which was held in June 2010.

Objective 1. To develop, evaluate, improve and integrate management techniques for soybean cyst nematode in the NCR to increase grower profitability.

Hundreds of soybean cultivars were evaluated for resistance to SCN in field and greenhouse trials in Illinois, Indiana, Iowa, Kansas, Ontario, and Tennessee during 2010. Results were published online (see publications) and distributed at numerous field days and extension meetings. HG Type Tests were conducted in Illinois, Kansas, Ontario, and Wisconsin. Results confirmed that the ability to reproduce on PI 88788, the most common source of resistance, is widespread among SCN populations in the North Central Region, occurring in 30% to 80% of sampled populations. Soybean cultivars with different sources of resistance were compared in several states. In Indiana, a 15 bu/a yield increase was observed for a cultivar with the Peking source of resistance, while in Arkansas, the PI437654 source of resistance provided greater nematode population reductions and greater yields than PI 88788 or Peking.

Objective 2. To better understand and apply the concepts of invasion biology as revealed by SCN epidemiology.

The Universities of Minnesota and Vermont are collaborating on a 4-year field experiment conducted in two locations in Waseca County in Southern Minnesota. The experiment is a split-plot design with no-till and conventional tillage as main plots, and five crop sequence-biocide treatments as subplots. Season 2 samples were collected at planting, midseason and harvest and analyzed. Treatment effects on the nematode suppressiveness were more evident in 2010 than 2009. Formaldehyde treatment of soil reduced fungal parasitism of SCN second-stage juveniles (J2) at planting and increased SCN population density at midseason. Conventional tillage resulted in greater SCN population density at midseason compared with no-till. Phosphatase and glycosidase were positively associated with soil suppressiveness to SCN.

In Michigan, the hypothesis that site-specific management is more appropriate than one-size-fits-all approach to managing parasitic variability was tested using two SCN populations in compost and commercial fertilizer amended sandy and sandy loam soils. In four greenhouse experiments, interaction effects of SCN population × amendment, SCN × soil type, amendment × soil type and/or SCN × amendment × soil type were statistically significant. The results suggest that best nematode management outcomes may not be achieved without considering the nematode population, the soil conditions and the management option together.

Objective 3. To develop a decision-support database for management of SCN and other regionally important nematodes.

A list of SCN-resistant soybean varieties available to Iowa growers was compiled in 2010. Soybean cultivar resistance ratings were published online in Illinois, Kansas, and Tennessee (see publications). Future databases will be posted on the NCSRP website www.planthealth.info.

Accomplishments

Short-term Outcomes: Data on the diversity of SCN virulence phenotypes across the region were shared. Additional unbiased information on SCN resistant varieties and soybean yield in relation to SCN population density was made available in 2010. Outputs: Publications are reported in the Publications section. Activities: Objective 1: Soybean cultivars were evaluated for SCN resistance and SCN virulence phenotypes were surveyed across the region. Rotations of soybean cultivars derived from different sources of resistance were compared. Objective 2: The second year of a multi-state experiment on soil suppressiveness was conducted. Objective 3: SCN resistance ratings for commercial soybean cultivars were compiled and published online. Milestones: An extensive dataset consisting of 3 years of observations on SCN resistance reactions among commercial soybean cultivars and the diversity of SCN virulence phenotypes has been compiled for the North Central Region. The prevalence of SCN populations that are virulent on PI 88788, the most common source of resistance, has been documented.

Publications

Journal Articles Bao, Y., Neher, D.A., and Chen, S. 2010. Effect of soil disturbance and biocides on nematode communities and extracellular enzyme activity in soybean cyst nematode suppressive soil. Nematology. DOI:10.1163/138855410X541230. Bernard, E.C., Handoo, Z.A., Powers, T.O., Donald, P.A., and Heinz, R.D. 2010. Vittatidera zeaphila (Nematoda:Heteroderidae), a new genus and species of cyst nematode parasitic on corn (Zea mays). Journal of Nematology 42:139-150. Delheimer, J. C., T. Niblack, M. Schmidt, G. Shannon, and B. W. Diers. 2010. Comparison of the effects in field tests of soybean cyst nematode resistance genes from different resistance sources. Crop Sci. 50: 2231-2239. Faghihi, J, P. A. Donald, G. Noel, T. Welacky, and V. R. Ferris. 2010. Soybean resistance to field populations of Heterodera glycines in selected geographic areas. Plant Health Progress. doi:10.1094/PHP-2010-0426-01-RS. Kim, M., D. L. Hyten, T.L. Niblack, and B. W. Diers. 2011. Stacking resistance alleles from wild and domestic soybean sources improves soybean cyst nematode resistance. Crop Science 51:934-943. Mekete, T., K. Reynolds, H. D. Lopez-Nicora, M. E. Gray, and T. L. Niblack. 2010. Plant-parasitic nematodes, potential pathogens of Miscanthus x giganteus and Panicum virgatum used for biofuels. Plant Disease 95:413-418. Mekete, T., K. Reynolds, H. D. Lopez-Nicora, M. E. Gray, and T. L. Niblack. 2010. Distribution and diversity of root-lesion nematode (Pratylenchus spp.) associated with Miscanthus x giganteus and Panicum virgatum used for biofuels and species identification in a multiplex polymerase chain reaction. Nematology DOI:10.1163/ 138855410X538153. Melakeberhan, H., A. Kravchenko, J. Dahl, and D. Warncke (2010). Effects of soil types and Meloidogyne hapla on the multi-purpose uses of arugula (Eruca sativa). Nematology, 12: 115-120. Melakeberhan, H. (2010). Assessing cross-disciplinary efficiency of soil amendments for agro-biologically, economically, and ecologically integrated soil health management. Journal of Nematology, 42: 73-77. Melito, S., Heuberger, A. L., Cook, D., Diers, B. W., MacGuidwin, A. E., and Bent, A. F. 2010. A nematode demographics assay in transgenic roots reveals no significant impacts of the Rhg1 locus LRR-Kinase on soybean cyst nematode resistance. BMC Plant Biology 2010, 10:104 http://www.biomedcentral.com/1471-2229/10/104 Mennan, S. and H. Melakeberhan (2010). Effect of biosolid amendment on populations of Meloidogyne hapla and soils with different textures and pHs. Bioresource Technology, 101:7169-7175. Pedersen, P., G. L. Tylka, A, Mallarino, A. E. MacGuidwin, N. C. Koval, and C. R. Grau. 2010. Correlation between soil pH, Heterodera glycines population densities, and soybean yield. Crop Science 50:1458-1464. Rotundo, J.L., G.L. Tylka, and P. Pedersen. 2010. Source of resistance affects soybean yield, yield components, and biomass accumulation in Heterodera glycines-infested fields. Crop Science 50:2565-2574. Schapaugh Jr., W.T., T. Todd, J. Reese, J. Diaz-Montano, J. Meng, and C.M. Smith. 2010. Registration of K1639-2 soybean germplasm resistant to soybean cyst nematode and soybean aphid. Journal of Plant Registrations 4:67-69. Schroeder, N. E., and MacGuidwin, A. E. 2010. Behavioral quiescence reduces the penetration and toxicity of exogenous compounds in second-stage juveniles of Heterodera glycines. Nematology, 12:277-287. Schroeder, N. E., and MacGuidwin, A. E. 2010. Mortality and behavior in Heterodera glycines juveniles following exposure to isothiocyanate compounds. J. Nematology 42:194-200. Abstracts Arias, M.M.D., G. Tylka, L. Leandro, and G. Munkvold. 2010. Effects of soybean cyst nematode infestation and resistance on Fusarium root rot on soybeans. Phytopathology volume 100, no. 6, suppl. 1, p. S30. Donald, P.A., and Tyler, D.D. 2010. Effects of tillage on the interaction of soybean rhizosphere organisms. Journal of Nematology (in press). Donald, P.A., Allen, P.B., Sistani, K., Tyler, D.D., and Tewolde, H. 2010. Effect of poultry litter on Heterodera glycines reproduction. Phytopathology 100:S30. Faghihi, Jamal, R. A. Vierling and V. R. Ferris. 2010. Effect of fall annual rye grass seeding on soybean cyst nematode. Journal of Nematology (in press). Lopez-Nicora, H. D., J.P. Craig, and T.L. Niblack. 2010. Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction. Abstracts of the Annual Meeting of the Society of Nematologists: 76. Journal of Nematology (in press). Lopez-Nicora, H. D., T. Mekete, K. Reynolds, M. E. Gray, and T.L. Niblack. 2010. Distribution and diversity of Pratylenchus spp. associated with biofuel crops and species identification in a multiplex PCR assay. Phytopathology 100:S74. MacGuidwin, A. E. 2010. Population dynamics of Pratylenchus penetrans on corn and the relationship of nematode population densities and corn yield. Society of Nematologists, Boise, Idaho. Journal of Nematology (in press). Mekete, Tesfamariam, Kimberly Reynolds, Horacio D. Lopez-Nicora, Michael E. Gray, and Terry L. Niblack. 2010. Development of species specific primers for molecular diagnostics of plant-parasitic nematodes associated with Miscanthus x giganteus and Panicum virgatum used for biofuels. Journal of Nematology (in press). Melakeberhan, H. (2010). Are we overlooking adaptation and interaction effects when managing nematode parasitic variability? 30th International Symposium of the European Society of Nematologists. 25. Nishanthan, T.and Neher, D.A. Ecology of nematode suppressive soils in Midwest soybean-cropping systems, 2011. Soil Ecology Society, Kelowna, British Columbia, Canada, 24-27 May 2011. Tylka, G.L. 2010. Maximizing effectiveness of extension education efforts. Proceedings of the 49th annual meeting of the Society of Nematologists, p. 117-118. Welacky, T.W., J. Faghihi, P. Donald, G. Noel, and V. Ferris. 2010. Soybean Resistance to field populations of Heterodera glycines in selected geographic areas. 30th International Symposium of the European Society of Nematologists Proceedings, p.139. Research Reports Allen, F.L., Johnson, R., Williams, R.C., Jr, McClure, A.T., Newman, M., Donald, P. Soybean Variety Performance Tests in Tennessee. 2010. http://varietytrials.tennessee.edu/pdffiles/2010trialdata/soybean_FINALrpt.pdf. Research Report 11-03. University of Tennessee Institute of Agriculture (Technical Bulletin). Andrews, B., SCN Management Update . 2010, Top Crop Manager, v36, no. 17. Cary, T. R. and Diers, B. 2010 Northern Regional Soybean Cyst Nematode Test report, Group I-IV. Urbana, IL : University of Illinois, Dept. of Crop Sciences. Esker, P., A. MacGuidwin, and R. Proost. 2010. Nematodes: The overlooked yield robbers of corn and soybean. UW Extension. Giesler, L. et al. 2010. Soybean Cyst Nematode Management : Understanding How Management Actions Influence Nematode Populations. http://www.planthealth.info/ LeBoeuf, Ontario Research & Development Program (ORDP), Project # ORD-008, Monitoring for early warning of new Sugarbeet Pests in Ontario. 2010 Interim Report. Niblack, T.L. 2010. SCN resistance ratings, in the Variety Information Program for Soybeans (VIPS) at www.vipsoybeans.org. Schmidt, M. Schmidt, C. et al. 2010. The Sudden Death Syndrome Regional Tests, Annual Report. North Central Soybean Research Program Report. Southern Illinois University of Carbondale, Carbondale, Illinois. Todd, T.C. and W.T. Schapaugh. Female indices and SDS ratings for the 2010 Kansas Soybean Performance Test entries. http://www.agronomy.ksu.edu/extension/~/doc3502.ashx Welacky, T.W. Annual Reports - Ontario Soybean Variety Trials. Ontario Oil and Protein Seed Crop Committee. 12p. Extension Publications Diaz Arias, M., G.L. Tylka, A. Robertson, L. Leandro, and G. Munkvold. 2010. Fusarium root rot of soybean - occurrence, impact, and relationship with soybean cyst nematode. Proceedings of the 2010 Iowa State University Integrated Crop Management Conference, pp. 115-123. Mueller, D., A. Robertson, A. Sisson, and G. Tylka. 2010. Soybean diseases. Iowa State University Extension Publication CSI 0004, 36 pp. Tylka, G.L. and M. P. Mullaney. 2010. Soybean cyst nematode-resistant soybeans for Iowa. Iowa State University Extension Publication PM 1649, 27 pp. Tylka, G.L., G.D. Gebhart, and C.C. Marett. 2010. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa - 2009. Iowa State University Extension, publication IPM 52. Tylka, G.L., G.D. Gebhart, C.C. Marett, M.P. Mullaney, and S.N. Wiggs. 2010. Evaluation of soybean varieties resistant to soybean cyst nematode in Iowa - 2010. Iowa State University Extension, publication IPM 52. Conference proceedings Tylka, G. 2010. Corn nematodes: what will 2010 hold for us? Proceedings of the Iowa State University Crop Advantage Series, page 13. Tylka, G. 2010. Nematodes that feed on corn - what to make of it all. Proceedings of the 2010 Iowa State University Integrated Crop Management Conference, pp. 97-101. Tylka, G. 2010. SCN: what will 2010 hold for us? Proceedings of the Iowa State University Crop Advantage Series, page 14. Tylka, G. 2010. The increase in SCN reproduction on resistant soybean varieties - what does it mean? Proceedings of the 2010 Iowa State University Integrated Crop Management Conference, pp. 103-105. Grant Proposals Funded Bissonette, S. CSREES Extension IPM Coordination and Support Program. 2009-2010. Corn Nematode Survey, Niblack, $37,596. Donald, P.A. Tennessee Soybean Promotion Board. Managing Soybean Cyst Nematode in High Risk Fields. Donald, P.A. United Soybean Board. Investigations into the occurrence, distribution and impact of nematodes in soybean fields in the southern United States. Faghihi, J. and V. R. Ferris. NCSRP: Improving Management or Soybean Cyst Nematode through Extension Demonstration and Outreach. $21,000 (to Purdue). Giesler, L. and C. Bradley. 2009-2010. Improving management of soybean cyst nematode through extension demonstration and outreach. North Central Soybean Research Program. Niblack, $57,000. Lambert, K.N., and Niblack, T. L. 2009-2010and 2010-2011. Application of biotechnology to control of the soybean cyst nematode. United Soybean Board. Niblack, $156,000. Lambert, K.N., T. Niblack, and B. Diers. 2010-2011. Molecular tracking of Glycine tomentella chromosomes to facilitate the introgression of disease resistance genes into soybean. Dow AgroSciences. $72,910. Lambert, K.N., T. Niblack, and B. Diers. 2010-2011. High-Resolution Evaluation of SCN Resistant Soybean Using Inbred Nematode Lines. $80,201. Dow AgroSciences. Niblack, T. L. 2010. Illinois Soybean Association, Soybean Cyst Nematode Managed Research Area. $250,000. Robbins, R.T. Control of New Soybean Cyst Nematode Races using Rotation of Different Roundup Ready and Conventional Soybean Varieties: Approved for 2011-13. Arkansas Soybean Promotion Board. Robbins, R.T. Commercialization and Discovery of Biocontrol Agents for Soybean Nematodes: Approved for 2010-12. Arkansas Soybean Promotion Board. Schapaugh, W.T., T.C. Todd, H.N. Trick, and J. Long. 2010-2011. Trait and Production Efficiency Enhancement in Soybean. Kansas Soybean Commission. $281,000 (Todd, $86,000). Welacky, T.W. Grain Farmers of Ontario - Practical management of Soybean Cyst Nematode - Part 2.

Impact Statements

Producers are using additional management tools for field populations of SCN across the NC region. We have provided these by demonstrating variability among geographic isolates and communicating to growers the importance of integrating different management tactics for SCN control. Working together, our group has provided a more complete picture of SCN variability and virulence in the region. This could not have happened if the group had been working independently.
Soybean breeders have objective and independent evaluation of the impact of SCN on commercially available soybean cultivars. This information serves as a guide for future breeding strategies and was obtained through interdisciplinary cooperation with agronomists and others.
The research community has a more refined understanding of conditions favorable for establishment of SCN. Favorable economic return for soybean promotes expansion of production into non-traditional soybean production areas which, in turn, favors expansion of the known distribution of SCN. Understanding of complex plant rhizosphere interactions of SCN with other nematodes, bacteria, fungi and soil microarthropods will lead to a better conceptualization of conditions which favor establishment and build up of SCN in production fields.