Annual/Termination Reports:

[08/04/2006] [08/10/2007] [08/05/2008] [07/08/2009] [09/10/2010]

Report Information

Participants

State Agricultural Experimental Stations Representative:
Southern Region - Vice-Chair (2006) J. C. Miller, Jr.
Western Region - J. Stark
North Central Region - D.S. Douches
Northeastern Region - Chairman (2006) W. De Jong

United States Department of Agriculture:
ARS - Technical Representative - Secretary (2006) C. R. Brown
ARS - National Program Staff - P. K. Bretting
ARS - Area Director, Midwest Area - S. Shafer
Cooperative States Research Education & Extension Service - A. M. Thro
Animal and Plant Health Inspection Service - L. E. Levy
Inter-Regional Potato Introduction Project - Project Leader J. B. Bamberg

Agriculture Canada - T. R. Tarn

Administrative Advisors:
Southern Region - R. L. Westerman
Western Region - C. Y. Hu
North Central Region - Lead S. A. Slack
Northeastern Region - S. D. Reiling

Brief Summary of Minutes

Attendees
C. Y. Hu (Western Rep), Walter De Jong (Northeast), Max Martin, Steve Slack, Dona Sklarczyk Gary Kinard, John Bamberg, Gary Kinard, Dave Spooner, Creighton Miller Southern), Jr., Steve Reilling , Murali Bandla,
John Bamberg made a presentation to Natonal Potato Council in the past year.

Jorge Coco Abad will replace Suzanne Hurtt shortly at Quarantine in Beltsville, MD..

Jeff Stark was selected to be the Western Rep, but declined in the end. Many members assumed that Isabel Vales was the rep. The West goes unrepresented this time.

Richard Guthrie will be representing the Southern Region as AA.

Agenda approved
Last years minutes approved
Resolution Committee established, Chuck Brown, Creighton Miller

Dave Spooner
Listed Projects and activities which are also in the report. Reciprocity issue for collecting is still not resolved. Nobody is collecting in Andean Countries.

Discussed single point of origin of potato from Northern Brevicaule complex. This means that the ancestry of all cultivated potato is from this source.

Subcontinent Indian landraces are of Chilean origin.

Domingo Ríos is in Tenerife and in charge of the Canaries potato germplasm.

Mexican landraces are recent based on lack of linguistic, archeaological, written records.

We discussed the ownership debates over germplasm with no new conclusions.

John Bamberg discussed his report.

The monthly reports were discussed and most members indicated that were informative.
Collecting: considering whether we should put more effort into collecting in hard to reach places.
Max Martin is in charge of disease abatement. PSTV. They use Agdia to test for it.
PSTV material might be better discarded.

Materials that come from off-station should be grown off-site first, particularly in regard to PSTV.

Jessy Schartner is engaged in germination research. Apomicitic phenomena are being studied as well .
Late fruit maturation is an occasional issue. Fruit remains small with corolla attached.

Evaluation: Goal is to make tools and opportunities for other researchers. Mutants. Nutritional issues. Anti-cancer factors, antioxidants, potassium. In other words, promote the good things about potato.

Frost resistance. Finishing that project. Sharing with CIP colleagues.

Developmental mutants. One example is development of sepals instead of the flower. Might be useful as a tool for studying floral developing.

Tuber pH in exotic species is a project. Effects on disease resistance, processing quality.

Solanum microdontum was chosen as a taxon for multiplexing study.

Use of neutral markers may not be the best markers to use. Sequence related to function will displace this in the future.

Is it hypothetically possible that a bioinformaticist should be functioning in collaboration with vast sequence resources.

For instance, determining the sequence of genes important in carbohydrate metabolism.

A discussion of the acceptance of transgenics ensued. No one seems to know how transgenic potato will become acceptable in North America, but it is on track for commercialization in India and China.

2005 was high point with a total of 20,000 units distributed.

Association of potato intergenebank collaborators (APIC) initiatives. John Bamberg traveled to CIP to reinvigorate this activity.

Walter De Jong reported on Northeast Region see Annual report.

Creighton Miller reported on Southern Region see Annual report.

We discussed the value of 4x-2x crosses that were originally seen as a means of inserting wild species genes into tetraploid germplasm.

4x -2x crosses have fallen by the wayside due to the difficulty of obtaining several combined traits and a high rate of 2n pollen production combined with fecundity.

Steve Slack made several comments on administrative matters.

Should we allow visitor participants at the meeting without them having official function.
One of the issues is who pays for it. The experiment station directors may not pay for official delegates or visitors.

There is an effort to remove formula funds and make them national competitive funds.
As projects end the money will be siphoned into a competitive grant system.
Multistate projects would go from 25% to 50% and become competitively funded.

House has indicated its support not to change the system. How many cycles will it have to go through before it is lost. Agricultural funding has not had an increase in twenty years.

NIH has a big hook to hang things on. Like Cancer.

Ag Research has many agendas with many groups pitching their topic of interest.

Don Sklarczyk reported on the history of the NPC and NPPB.
The National Potato Council has John Keeling and two staff members. Growers are all volunteer based made up of five vice presidents. The organization is heavily dependent on grower volunteers.
Levies to support these organization are 1.5 cents and 2.0 cents for NPC and NPPB, respectively. NPC seeks funds from other sources. Friends of the Industry. NPC can get grants. Du Pont supplies $10 k per year. Very active group. Make sure that committees are very active.

NPC tries to provide one voice for the potato industry. John has interacted on the new farm bill. Specialty crops group was formed. Cohesive voice for specialty crops in the new farm bill. Would like to see funding equity 50% comes from specialty crops and only 3% of farm payments comes back. Like to see more money to come back into development and research programs.

NPPB has to make sure that anything they do is not construed as lobbying because they are under federal oversight.

Budget Talk

The reduction of funding means that there will be a conversion of personnel to Federal employment. Also the manner in which funding will be offset by federal funds is unscheduled.

Removing employees from state positions means they are terminated from that position, and placement in a federal appointment means that they start a new job. There is no way to make all forms of remuneration equivalent. NRSP-6 funding of travel costs may need to be reduced. It is never in the budget.

Meeting in Sturgeon Bay in 2007. June 19 and 20. Tuesday and Wednesday.

Dave Douches was elected secretary for 2007.

Secretarys Review
1. Advocate that NRSP-6 study the possible change in activities (additional activities) in relation to the availability of extensive sequence once the potato genome is fully sequenced.
2. Description of single point of origin research lead to clarification of implications for germplasm workers.
3. Whether potato in higher latitudes comes from Andes or Southern Chile is still an active area of debate. There are implications on germplasm improvement.
4. Discussion of what to do with PSTV infected material.
5. Discussion of retirement of Suzanne Hurtt and accession to the position of Jorge Abad.
6. NPC and NPPB are important organizations that do a lot with small number of staff. We are grateful that they do that.
7. Emphasize that Quarantine servicing of potato germplasm needs is good, and in vitro introduction immediately and clean up service and facilitated a number of research goals. We would like Jorge Abad to come to our meetings
8. Budget discussions center on how to adjust to funding reductions that may or may not be made up for by ARS increases.

Resolution:
Whereas the NRSP-6 committee enjoyed wonderful hospitality and facilities; and
Whereas Candice Gardner was an exemplary host; and
Whereas all participants learned a great deal and was able to impart knowledge in a comfortable and beautiful setting; and
Whereas the tour of facilities was well-organized and technically rich; and
Whereas the staff that carries out the research and curatorial activities does an outstanding job; and
Whereas David Spooner is a pre-eminent world authority on potato taxonomy and John Bamberg is an outstanding researcher and curator of North Americas Potato Germplasm Collection; and
Whereas Bob Westerman served as Adminstrative Advisor from the Southern
Be it hereby resolved
That TAC expresses its gratitude and sincere appreciation to all these individuals.

A motion That the national potato council representative be an official voting member of the TAC. By Creighton Miller seconded by Walter De Jong. Passed.

Respectfully submitted,
Charles R. Brown
Secretary 2006

Accomplishments

A. Introduction of New Stocks<br /> Dr. Bamberg, Dr. Alfonso del Rio, and Charles Fernandez (US Potato Genebank) had a successful collecting expedition to the Huachuca Wilderness in southeast Arizona and the Santa Catalina Mountains near Tucson, Arizona in September of 2005 (supported with extramural funding from USDA). They collected 14 new accessions, 13 of S. stoloniferum and one S. jamesii from the Chiricahua Wilderness. For 50 years the only available germplasm from this area consisted of a few collections from spots easily accessible by road. New collections were from sites accessible only through camping and extensive hiking. This will be a test case of an important question about germplasm collecting: Do samples from sites easy to access adequately represent the genetics in a geographic area, or is there much more work that should be done to access more remote sites? They also collected two S. stolonifenum from the Santa Catalina Mountains, both near two previous herbarium collection sites.<br /> A total of 22 accessions were assigned PI numbers in 2005: two PVY resistant varieties from the UK, five late blight resistant clones from Mexico, one S. soestii from Bolivia, and 14 accessions collected from the SW United States. These accessions are now available from the NRSP-6 Solanum germplasm collection. <br /> Several steps were taken in the past year to promote and inform researchers of NRSP-6s services and stocks. The NRSP-6 web page (http://www.ars-grin.gov/nr6) has been updated and made more user friendly. Clientele that have ordered from NRSP-6 in the past four years are contacted three times per year informing them of new materials that are now available either as true seed, tubers, in vitro plantlets, or herbarium samples. A new service we are offering is dried ground leaf samples for DNA testing. For foreign requesters this is useful since there are no quarantine restrictions on dried material. <br /> <br /> B. Preservation and Increase of Stocks<br /> In 2005, a total of 175 accessions were increased as botanical seed populations.<br /> A total of 900 potato spindle tuber viroid (PSTVd) tests were performed on seed increase parents, seedlots and research materials. Germination tests were performed on 1199 accessions, ploidy determinations were made on nine accessions, and tetrazolium seed viability tests were done on 100 seedlots. <br /> Progress was made on several international collaborative projects. We are working with CIP on a project to assess the impact of agrichemicals on pollen and seed viability of wild species (oral presentation at the PAA06). We planned a study to look at the prevalence of hidden recessives and diversity of nematode resistance alleles in S. andigena with Russian (VIR) colleagues.<br /> Work continued with Dr. C. Millers lab screening for antioxidants in uncolored wild potato species tubers. Previous screening among species identified ones with promising levels of tuber antioxidant capacity. Some diploid Mexican species seem to have high antioxidants in common: S. cardiophyllum, S. jamesii, and S. pinnatisectum (paper submitted AJPR 3/2006). Continued fine screening is discovering high levels of antioxidants in species that are more crossable to tuberosum and known to have acceptably low levels of glycoalkaloids. <br /> We explored the potential of using a potato-based in vitro Agrobacterium tumefaciens system to screen for anti-tumor factors. The Potato Crop Germplasm Committee (CGC) also sponsored a funded grant proposal to screen for Potato Carboxypeptidase Inhibitor (PCI), a small heat-stable protein unique to potato that has been shown to efficiently inhibit proliferation of some of the most pernicious human cancers.<br /> Frost resistant breeding work continues with recurrent selection of S. tuberosum, S. acaule and S. commersonii hybrids. Materials are now maturing earlier with the introgression of very early varieties, and some selections have been confirmed to survive in vitro leaf freezing to 5oC. In a related study, substitution backcross families were used to show that cytoplasm is not a factor in the extreme frost hardiness of S. commersonii. <br /> We discovered a new floral development mutant in S. microdontum which we have named crazy sepal because it grows multiple (indeterminate) sets of sepals instead of anthers and pistils. This is a potential tool for studying floral development, and may have other applications as a completely sterile mutant. Progeny tests indicate monogenic recessive gene action. Attempts to artificially restore fertility are in progress as well as the generation of DNA markers to tag the recessive cs1 allele (short communication in AJPR).<br /> Testing was continued in an effort to confirm and characterize apomixis in S. jamesii. <br /> A field tuberization location in Hawaii was tested and appears to be effective. Until now, the only other way to produce tubers of wild species has been in winter greenhouse pots, and such tubers might not give a true indication of their potential value with respect to a variety of tuber traits.<br /> Plant parts are known to vary widely for pH, but little is known about the variation, basis, or utility of pH extremes in potato tubers. When pH was measured on species of the mini-core collection, replicates over years and populations gave rather consistent results, with S. polytrichon, S. pinnatisectum and S. papita being the most acidic. Cultivated species, S. verrucosum and S. okadae, were least acidic. The observed range of pH (5.5-6.1) represents a 4-fold difference in H ion concentration. We intend to fine-screen among genotypes within species to find the limits of tuber acidity, then characterize the physiology, genetics and impact on economic traits like disease resistances and processing quality (oral presentation at the PAA06).<br /> We want to see if we can do more efficient research by combining many initiatives. Solanum microdontum is remarkably extreme and variable for several characteristics (acid, potassium, calcium, late blight, and antioxidants). We will characterize all the S. mcd for these traits. We also want to collect and analyze DNA to see if gross genetic differences are a guide to variation for useful traits.<br /> <br /> C. Classification <br /> Dr. Spooner et al. have published and are working on five different areas of potato research: 1) a manual on the use of molecular markers for genebank studies, 2) on studies in cultivated potato origins, 3) on molecular investigations of relationships in wild tomatoes and potatoes, 4)on an exploration of the predictive power of taxonomy relative to disease resistance data, 5) on a molecular linkage map of late blight in wild potatoes. <br /> <br /> D. Distribution<br /> NRSP-6 distributed 3,929 units of seed (not including in-house use); 135 tuber families; 1,106 tuber clones; 732 in vitro stocks; 368 DNA samples; and 157 plants in plugs to clientele in 30 states of the USA and 12 other countries. Internally NRSP-6 used 13,566 units of seed for seed backup, chromosome counts, germination tests, identification and taxonomic check plantings, in vitro maintenance, seed increases, PSTV tests, and miscellaneous plantings. The volume and types of stocks sent to various consignee categories are summarized in the table which is included in the attachment with the minutes.<br />

Publications

Bamberg, J.B., J.P. Palta, and S.E. Vega. 2005. Solanum commersonii cytoplasm does not improve freezing tolerance in substitution backcross hybrids with frost-sensitive potato species. Am. J. Potato Res. 82:251-254.<br /> <br /> Busse, J., J.B. Bamberg and J.P. Palta. 2005. Genetic variations for calcium accumulation efficiency in tuber and aerial shoot tissue. Am. J. Potato Res. 82:60. (Abstract)<br /> <br /> Del Rio, Alfonso, J.B. Bamberg and C. Fernandez. 2005. Assessment of the genetic structure of in situ populations of wild potato Solanum fendleri eco-geographically dispersed in the Chiricahua Mountains, Arizona, USA. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> del Rio, A.H., J.B. Bamberg and Z. Huaman. 2005. Assessment of putative identical germplasm collections at CIP and US Potato genebanks determined by RAPD and SSR markers. Am. J. Potato Res. 82:66. (Abstract)<br /> <br /> Fernandez, C.J. and J.B. Bamberg. 2005. A new Solanum fendleri mutant lacking purple pigment. Am. J. Potato Res. 82:69. (Abstract)<br /> <br /> Kiru, S., S. Makovskaya, J. Bamberg, and A. del Rio. 2005. New sources of resistance to race Ro1 of the Golden nematode (Globodera rostochiensis Woll.) among reputed duplicate germplasm accessions of Solanum tuberosum L. subsp. andigena (Juz. et Buk.) Hawkes in the VIR (Russian) and US Potato Genebanks. Genet. Resources and Crop Evol. 52:145-149.<br /> <br /> Lara-Cabrera, S. and D.M. Spooner. 2005. Taxonomy of Mexican diploid wild potatoes: (Solanum sect. Petota) morphological and microsatellite data. Monogr. Syst. Bot., Missouri Bot. Gard. 104:199-205.<br /> <br /> Lozoya-Saldana, H., O. Barrios and J. Bamberg. 2005. Phytophthora infestans; races vs genotypes in the Toluca Valley, Mexico. Am. J. Potato Res. 83:122. (Abstract)<br /> <br /> Nzaramba, Ndambe M., John Bamberg, Douglas C. Scheuring, and J. Creighton Miller, Jr. 2005. Antioxidant activity in Solanum species as influenced by seed type and growing location. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Spooner, D.M. and W.L.A. Hetterscheid. 2005. Origins, evolution, and group classification of cultivated potatoes. In: Darwins Harvest: New Approaches to the Origins, Evolution, and Conservation of Crops. T.J. Motley, N. Zerega, and H. Cross (eds.). pp. 285-307. Colombia University Press, New York.<br /> <br /> Spooner, D.M., K. McLean, G. Ramsay, R. Waugh, and G.J. Bryan. 2005. A single domestication for potato based on multilocus AFLP genotyping. Proc. Natl. Acad. Sci. USA. 120:14694-14699.<br /> <br /> Spooner, D.M., J. Nunez, F. Rodriguez, P.S. Naik, and M. Ghislain. 2005. Nuclear and chloroplast DNA reassessment of the origin of Indian potato varieties and its implications for the origin of the early European potato. Theor. Appl. Genet. 110:1020-1026.<br /> <br /> Spooner, D.M., I. Peralta and S. Knapp. 2005. AFLP phylogeny of wild tomatoes (Solanum L. section Lycopersicon [Mill.] Wettst. subsection Lycopersicon. Taxon. 54:43-61.<br /> <br /> Vega, S.E., J.B. Bamberg and J.P. Palta. 2005. Characterization of gibberellin requirements for various diploid and tetraploid gibberellin deficient mutants. Am. J. Potato Res. 82:94. (Abstract)<br /> <br /> Vega, S.E., J.P. Palta and J.B. Bamberg. 2005. Evidence for the mitigation of gibberellin deficiency symptoms by root zone calcium in GA-deficient mutants of potato. Am. J. Potato Res. 82:94-95. (Abstract)<br /> <br /> Villamon, F.G., D.M. Spooner, M. Orillo, E. Mihovilovich, W. Perez, and M. Bonierbale. 2005. Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana). Theor. Appl. Genet. 111:1201-1214. <br /> <br /> A. Journal Articles and Abstracts Reporting Research with NRSP-6 Stocks<br /> <br /> Aversano, R., M.J. Sanchez, D. Carputo, L. Frusciante, and J.M. Bradeen. 2005. Molecular strategies for the efficient characterization of resistance gene marker and allelic diversity in Solanum species. Solanaceae Genome Workshop, Ischia, Italy, September 2005. (Abstract)<br /> <br /> Aversano, R., R.L. Syverson, E.A. Quirin, D. Carputo, and J.M. Bradeen. 2005. Development of integrated molecular marker resources for resistance gene mapping and exploration of resistance gene diversity in the genus Solanum. Presented at Annual Congress Societa Italiana di Genetica Agraria XLIX, Potenza, September 12-15, 2005. (Abstract)<br /> <br /> Aversano, R., R.L. Syverson, E.A. Quirin, D. Carputo, and J.M. Bradeen. 2005. R gene mapping and isolation in the genus Solanum: Towards an expandable, communal resource of integrated physical and genetic maps for candidate R genes. Presented at Triennial Conference of the European Assoc. for Potato Research XVI, Bilbao, Spain, July 2005. (Abstract)<br /> <br /> Aversano, R., E.A. Quirin, R.L. Syverson, D. Carputo, and J.M. Bradeen. 2005. Development of integrated Solanum bulbocastanum genetic and physical maps as a communal resource for mapping and isolation of R genes. Presented at Plant & Animal Genome XIII, San Diego, CA, January 2005. (Abstract)<br /> <br /> Belknap, W.R. 2005. Modification of potato alkaloids- a lesson in applied metabolomics. Presented at American Chemical Society, Agrochemicals Division Symposium, ACS National Meeting, San Diego, California, March 2005. (Abstract)<br /> <br /> Bisognin, D.A., D.S. Douches, L. Buszka, G. Bryan, and D. Wang. 2005. Mapping late blight resistance in Solanum microdontum Bitter. Crop Sci. 45:340-345.<br /> <br /> Boltowicz, D., A. Szczerbakowa and B. Wielgat. 2005. RAPD analysis of the interspecific somatic hybrids Solanum bulbocastanum (+) S. tuberosum. Cell. Mol. Bio. Letters 10(1):151-162.<br /> <br /> Braden, J.M., M.J. Sanchez, R.L. Syverson, R. Aversano, D.S. Mollov, and D. Carputo. 2005. Understanding molecular diversity: towards strategic sampling of genebank collections. Solanaceae Genome Workshop, Ischia, Italy, September 2005. (Abstract)<br /> <br /> Brown, C.R. 2005. Antioxidants in Potato. Am. J. Potato Res. 82:163-172.<br /> <br /> Brown, C.R., D. Culley, C.P. Yang, R. Durst, and R. Wrolstad. 2005. Variation of anthocyanins and Carotenoid contents and associated antioxidant values in potato breeding lines. J. Am. Soc. Hort. Sci. 130:174-180.<br /> <br /> Brown, C.R., W. De Jong and C.-P. Yang. 2005. Inheritance of total Carotenoid in high-content diploid germplasm. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Bu QingYun, Wu Liang, Yang ShiHu, and Wan JianMin. 2005. Cloning, characterization and expression vector construction of potato protease-inhibitor II gene (PIN II-2x) from diploid potato (Solanum phureja). Hereditas 27:417-422.<br /> <br /> Burkhart, C.R., B.J. Christ and K.G. Haynes. 2005. The relative virulence of various isolates of Fusarium species on potato. Presented at the American Phytopathology Meeting 2005.<br /> <br /> Burkhart, C.R., B.J. Christ and K.G. Haynes. 2005. Heritability of resistance to Fusarium dry rot in a diploid hybrid potato population. Presented at the American Phytopathology Meeting 2005.<br /> <br /> Carputo, D. and A. Barone. 2005. Ploidy level manipulations in potato through sexual hybridization. Annals of Appl. Bio. 146:71-79.<br /> <br /> Chen, Qin and H.Y. Li. 2005. An improved technique for high-resolution mitotic chromosome studies in Solanum. HortSci. 40:54-56.<br /> <br /> Chen, Q., H.B. Zhang and D. De Koeyer. 2005. Development of genomic tools for large-scale physical mapping and map-based cloning of disease and insect resistance genes in potato. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Costanzo, S., I. Simko, B.J. Christ, and K.G. Haynes. 2005. QTL analysis of late blight resistance in a diploid potato family of Solanum phureja x S. stenotomum. Theor. Appl. Genet. 111:609-617.<br /> <br /> Davenport, J.R., P.H. Milburn, C.J. Rosen, and R.E. Thornton. 2005. Environmental impacts of potato nutrient management. Am. J. Potato Res. 82:321-328.<br /> <br /> Davis, Jeffrey A., Edward B. Radcliffe, David W. Ragsdale, and Christian A. Thill. 2005. Identifying resistance to aphids in crosses with somatic fusions of Solanum tuberosum L. and Solanum bulbocastanum Dun. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> De Koeyer, D., Y. Pelletier, D. Ronis, C. Clark, and V. Burns. 2005. Solanum oplocense: A new source of resistance to Colorado potato beetle and processing quality attributes. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Dinu, I.I., R.J. Hayes, R.G. Kynast, R.L. Phillips, and C.A. Thill. 2005. Novel inter-series hybrids in Solanum, section Petota. Theor. Appl. Genet. 110:403-415.<br /> <br /> Estrada, Maria A. and David S. Douches. 2005. Potato tuberworm (Lepidoptera: Gelichiidae) resistance in potato lines with the Bacillus thuringiensis-cry1Ac gene and natural resistance factors. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Feingold, S., J. Lloyd, N. Norero, M. Bonierbale, and J. Lorenzen. 2005. Mapping and characterization of new EST-derived microsatellites for potato (Solanum tuberosum L.). Theor. Appl. Genet. 111:456-466.<br /> <br /> Flis, B., J. Hennig, D. Strzelczyk-Zyta, C. Gebhardt, and W. Marczewski. 2005. The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars. Molecular Breeding 15:95-101.<br /> <br /> Ganga, Z.N., G.A. Porter, D. Lambert, G. Sewell, and A. Bushway. 2005. Reeves Kingpin: A high yielding mid-season variety suitable for fry processing. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Germain, H., E. Chevalier, S. Caron, and D.P. Matton. 2005. Characterization of five RALF-like genes from Solanum chacoense provides support for a developmental role in plants. Planta 220:447-454.<br /> <br /> Gounaris, Y. 2005. Asymmetric potato cybrids derived from protoplast fusion between the selection ND860-2 and the cultivar Russet Burbank. J. Food, Ag. Environ. 3:157-160.<br /> <br /> Groza, H.I., B.D. Bowen, D. Kichefski, S.J. Peloquin, W.R. Stevenson, A.J. Bussan, and J. Jiang. 2005. Millennium Russet: A dual purpose russet potato variety. Am. J. Potato Res. 82:211-219.<br /> <br /> Hayes, R.J., I.I. Dinu and C.A. Thill. 2005. Unilateral and bilateral hybridization barriers in inter-series crosses of 4x 2EBN Solanum stoloniferum, S. pinnatisectum, S. cardiophyllum, and 2x 2EBN S. tuberosum haploids and haploid-species hybrids. Sexual Plant Reproduction 17:303-311.<br /> <br /> Haynes, K.G. and B.J. Christ. 2005. Improvements in foliar late blight resistance in a diploid hybrid Solanum phureja  S. stenotomum population. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Haynes, K.G., B.J. Christ, R.T. Zink, R.D. Davidson, and J.S. Miller. 2005. Powdery scab trials of potato varieties and advanced selections in 2003. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Ishizaki, T. and A. Kato. 2005. Introduction of the tobacco Retrotransposon Tto1 into diploid potato. Plant Cell Reports 24(1):52-58.<br /> <br /> Jung, C.S., H.M. Griffiths, D.M. De Jong, S. Cheng, M. Bodis, and W.S. De Jong. 2005. The potato P locus codes for flavonoid 3,5-hydroxylase. Theor. Appl. Genetics 110:269-275.<br /> <br /> Khu, D.M., S.L. Love and J.H. Lorenzen. 2005. Identification of AFLP and SSR markers associated with corky ringspot disease resistance in a tetraploid population (Solanum tuberosum ssp. tuberosum). Am. J. Potato Res. 83:117. (Abstract)<br /> <br /> Kim-Lee, Neiyoung, J.S. Moon, Y.J. Hong, M.S. Kim, and H.M. Cho. 2005. Bacterial wilt resistance in the progenies of the fusion hybrids between haploid of potato and Solanum commersonii. Am. J. Potato Res. 82:129-137.<br /> <br /> Kirk, W.W., F.M. Abu-El Samena, J.B. Muhinyuzaa, R. Hammerschmidt, D.S. Douches, C.A. Thill, H. Groza, and A.L. Thompson. 2005. Evaluation of potato late blight management utilizing host plant resistance and reduced rates and frequencies of fungicide applications. Crop Protection 24:961-970.<br /> <br /> Kuhl, Joseph C. and D.S. Douches. 2005. Characterization of RB transgenic potato lines. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Lambert, D.H., M.L. Powelson, and W.R. Stevenson. 2005. Nutritional interactions influencing diseases of potato. Am. J. Potato Res. 82:309-319.<br /> <br /> Lebecka, R., E. Nimnoch-Guzowska, and Z. Kaczmarek. 2005. Resistance to soft rot (Erwinia carotovora subsp. atroseptica) in tetraploid potato families obtained from 4x-2x crosses. Am. J. Potato Res. 82:203-210.<br /> <br /> Lehesranta, S.J., H.V. Davies, L.V.T. Shepherd, N. Nunan, J.W. McNicol, S. Auriola, K.M. Koistinen, S. Suomalainen, H.I. Kokko, and S.O. Karenlampi. 2005. Comparison of tuber proteomes of potato varieties, landraces, and genetically modified lines. Plant Physio. 138:1690-1699.<br /> <br /> Leroux, V., C. Vincent, E. Lucas, Y. Pelletier, D. Quiring, and P. Giordanengo. 2005. Aphid resistance in wild potatoes: A laboratory study. Presented at European Association for Potato Research Triennial Meeting, Bilbao, Spain, July 17-22, 2005. (Abstract)<br /> <br /> Li, H.Y., Q. Chen, D. Beasley, and M. Goettel. 2005. Karyotype and evaluation analysis of Mexican wild species and tetraploid potato Solanum tuberosum by fluorescence in situ hybridization. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Li X-Q, H. De Jong, D.M. De Jong, and W.S. De Jong. 2005. Inheritance and genetic mapping of tuber eye depth in cultivated diploid potatoes. Theor. Appl. Genet. 110:1068-1073.<br /> <br /> Love, S.L., R. Novy, J. Whitworth, D.L. Corsini, J.J. Pavek, A.R. Mosley, R.E. Thornton, N.R. Knowles, S.R. James, and D.C. Hane. 2005. Summit Russet: A new russet potato variety with good fresh market and frozen processing qualities. Am. J. Potato Res. 82:425-432.<br /> <br /> McCue, K.F., L.V.T. Shepherd, P.V. Allen, M.M. Maccree, D.R. Rockhold, D.L. Corsini, H.V. Davies, and W.R. Belknap. 2005. Metabolic compensation of steroidal glycoalkaloid biosynthesis in transgenic potato tubers: using reverse genetics to confirm the in vivo enzyme function of a steroidal alkaloid glycosyltransferase. Plant Science 168:267-273.<br /> <br /> McCue, K.F., P.V. Allen, L.V. Shepherd, A. Blake, J. Whitworth, M.M. Maccree, D.R. Rockhold, D. Stewart, H.V. Davies, and W.R. Belknap. 2005. The primary in vivo steroidal alkaloid glucosyltransferase from potato. Phytochemistry. In Press.<br /> <br /> McCue, K.F., P.V. Allen, L.V. Shepherd, A. Blake, J.L. Whitworth, D.R. Rockhold, D. Stewart, H.V. Davies, and W.R. Belknap. 2005. Identification and characterization of a novel UDP-glucose: solanidine glucosyltransferase at the committed step in steroidal glycoalkaloid biosynthesis in potatoes. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Medeiros, A.H., I. DeLalibera, Jr. and W.M. Tingey. 2005. Aspects of potato leafhopper (Homoptera: Cicadellidae) biology on Solanum berthaultii and other potato genotypes. J. Econ. Entomol. 98:1704-1709.<br /> <br /> Meyer, S., A. Nagel and C. Gebhardt. 2005. PoMaMo  a comprehensive database for potato genome data. Nucleic Acids Research 33 (Database issue) <br /> <br /> Miller Jr., J.C., D.C. Sheuring, J.W. Koym, and D.G. Holm. 2005. TX1523-1Ru/Y a.k.a. Sierra GoldTM: An early maturing, yellow-fleshed russet cultivar for the specialty/gourmet market. Am. J. Potato Res. 82:369-377.<br /> <br /> Millett, B.P. and J.M. Bradeen. 2005. Does foliar blight resistance R gene RB function for tuber blight control? Presented at Plant & Animal Genome XIII, San Diego, CA, January 2005. (Abstract)<br /> <br /> Mueller, L.A., T.H. Solow, N. Taylor, B. Skwarecki, R. Buels, J. Binns, C.W. Lin, M.H. Wright, R. Ahrens, Y. Wang, E.V. Herbst, E.R. Keyder, N. Menda, D. Zamir, and S.D. Tanksley. 2005. The SOL Genomics Network. A comparative resource for Solanaceae biology and beyond. Plant Phys. 138:1310-1317.<br /> <br /> Narayanan, Karthikeyan and Gefu Wang-Pruski. 2005. Gene expression studies on genetic control of after-cooking darkening in diploid potatoes. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Navarre, Roy, R. Shakya and J. Holden. 2005. Analysis of tuber phenolics compounds in diverse potato germplasm. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Novy, R.G., J.S. Miller, L.D. Porter, S. Yilma, and A.R. Mosley. 2005. Identification and use of Solanum tuberosum subsp. andigena clones having both foliar and tuber resistance to late blight. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract) <br /> <br /> Novy, Richard G., J.M. Alvarez, S.B. Sterret, T.P. Kuhar, and D. Horton. 2005. Progeny of a tri-species potato somatic hybrid express resistance to wireworm in Eastern and Western potato production regions of the U.S. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Abstract)<br /> <br /> Novy, Richard G., S.L. Love, D.L. Corsini, J.J. Pavek, J.L. Whitworth, A.R. Mosley, S.R. James, D.C. Hane, C.C. Shock, K.A. Rykbost, C.R. Brown, R.E. Thornton, N.R. Knowles, M.J. Pavek, N. Olsen, and D.A. Inglis. 2005. Defender: A high-yielding, processing potato cultivar with foliar and tuber resistance to late blight. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Osumi, T., W.R. Belknap, D.R. Rockhold, M. Maccree, and K.L. Deahl. 2005. Patent approval 11/02/05, Solanum bulbocastanum late blight resistance gene and use thereof #10/647,268.<br /> <br /> Oyarzun, P.J., J. Yanez and G.A. Forbes. 2005. Evidence for host mediation of preinfection stages of Phytophthora infestans on the leaf surface of Solanum phureja. J. Phytopath. 152:651-657.<br /> <br /> Ozgen, S. and J.P. Palta. 2005. Supplemental calcium application influences potato tuber number and size. HortSci. 40:102-105.<br /> <br /> Park, T.H., V.G.A.A. Vleeshouwers, D.J. Huigen, E.A.G. van der Vossen, H.J. van Eck, and R.G.F. Visser. 2005. Characterization and high-resolution mapping of a late blight resistance locus similar to R2 in potato. Theor. Appl. Genet. 111:591-597.<br /> <br /> Park, TaeHo, J. Gros, A. Sikkema, V.G.A.A. Vleeshouwers, M. Muskens, S. Allefs, E. Jacobsen, R.G.F. Visser, and E.A.G. van der Vossen. 2005. The late blight resistance locus Rpi-blb3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato. Mol. Pl.-Mic. Inter. 18:722-729.<br /> <br /> Pelletier, Y. 2005. Resistance of a wild potato to the Colorado potato beetle. Presented at the Annual Meeting of the Acadian Entomological Society, Fredericton, NB, Canada, June 19-21, 2005. (Abstract)<br /> <br /> Pelletier, Y. and C. Clark. 2005. Use of reciprocal grafts to elucidate mode of resistance to Colorado potato beetle (Leptinotarsa decemlineata (Say)) and potato aphid (Macrosiphum euphorbiae Thomas) in six wild Solanum species. Presented at European Association for Potato Research Triennial Meeting, Bilbao, Spain, July 17-22, 2005. (Poster)<br /> <br /> Pelletier, Y., C. Clark and D. de Koeyer. 2005. The use of Solanum oplocense as a source of resistance to the Colorado potato beetle, Leptinotarsa decemlineata. Presented at European Association for Potato Research Triennial Meeting, Bilbao, Spain, July 17-22, 2005. (Abstract)<br /> <br /> Pelletier, Y., P. Dexter, R. Coffin, M. Bejan, E. Lucas, C. Vincent, V. Leroux, and P. Giordanengo. 2005. Aphid resistance in wild potatoes: Preliminary results of field and laboratory trials. Presented at the Annual Meeting of the Entomological Society of America, Canmore, Alberta, Canada, November 2-5, 2005. (Poster)<br /> <br /> Pelletier, Y., P. Dexter, R. Coffin, M. Bejan, E. Lucas, C. Vincent, V. Leroux, and P. Giordanengo. 2005. Aphid resistance in wild potatoes: Preliminary results on colonization and population development. Presented at European Association for Potato Research Triennial Meeting, Bilbao, Spain, July 17-22, 2005. (Poster)<br /> <br /> Pelletier, Y., J. Dutheil and D. de Koeyer. 2005. Mode of resistance to the Colorado potato beetle (Leptinotarsa decemlineata) of Solanum tarijense. Presented at European Association for Potato Research Triennial Meeting, Bilbao, Spain, July 17-22, 2005. (Abstract)<br /> <br /> Pianzzola, M.J., L. Zarantonelli, G. Gonzalez, L.F. Fraguas, and A. Vazquez. 2005. Genetic, phytochemical and biochemical analyses as tools for biodiversity evaluation of wild accessions of Solanum commersonii. Biochem. Syst. Eco. 33:67-78.<br /> <br /> Qin Xike, J. Soulard, G. Laublin, D. Morese, and M. Cappadocia. 2005. Molecular analysis of the conserved C4 region of the S11-RNase of Solanum chacoense. Planta 221:531-537.<br /> <br /> Quirin, E.A. and J.M. Bradeen. 2005. Towards the optimization of resistance gene analog discovery in Solanum species. Presented at Plant & Animal Genome XIII, San Diego, CA, January 2005. (Abstract)<br /> <br /> Raimondi, J.P., I.E. Peralta, R.W. Masuelli, S. Feingold, and E.L. Camadro. 2005. Examination of the hybrid origin of the wild potato Solanum ruiz-lealii Brucher. Plant Syst. Evol. 253:33-51.<br /> <br /> Reyes, L.F., J.C. Miller, Jr., and L. Cisneros-Zevallos. 2005. Antioxidant capacity, anthocyanins and total phenolics in purple- and red-fleshed potato (Solanum tuberosum L.) genotypes. Am. J. Potato Res. 82:271-277.<br /> <br /> Sanchez, Maria J. and James M. Bradeen. 2005. Towards efficient isolation of R gene orthologs from multiple genotypes: optimization of Long Range-PCR. Molecular Breeding. (In press)<br /> <br /> Simko, I., S. Costanzo, V. Ramanjulu, B.J. Christ, and K.G. Haynes. 2005. Mapping polygenes for tuber resistance to late blight in a diploid Solanum phureja  S. stenotomum hybrid population. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Sowokinos, J.S. Gupta and C.A. Thill. 2005. Introduction of an anti-sweetening gene (UgpA) from the cultivar Snowden into chipping cultivar Dakota Pearl. Presented at XVI Triennial Conference of the European Assoc. for Potato Research, Bilbao, Spain.<br /> <br /> Stark, J.C. and G.A. Porter. 2005. Potato nutrient management in sustainable cropping systems. Am. J. Potato Res. 82:329-338.<br /> <br /> Sterrett, S.B., K.G. Haynes and Dennis J. Timlin. 2005. Temperature regime in growth chamber identified for expression of internal heat necrosis. Presented at 89th Annual Meeting of The Potato Association of America, Calgary, Canada, July 17-21, 2005. (Poster Abstract)<br /> <br /> Sukhotu, T., O. Kamijima and K. Hosaka. 2005. Genetic diversity of the Andean tetraploid cultivated potato (Solanum tuberosum L. subsp. andigena Hawkes) evaluated by chloroplast and nuclear DNA markers. Genome 48:55-64.<br /> <br /> Szczerbakowa, A., D. Boltowicz, R. Lebecka, P. Radomski, and B. Wielgat. 2005. Characteristics of the interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum H-8105. Acta Phys. Plant. 27(3(A)):265-273.<br /> <br /> Thill, C.A. and I.I. Dinu. 2005. Examining chromosomal interactions in inter-series hybrids of Solanum ssp, section Petota. Presented at XVI Triennial Conference of the European Assoc. for Potato Research, Bilbao, Spain.<br /> <br /> Thill, C.A. and J. Miller. 2005. Minnesota potato breeding program progress report-2005. Proc. of the 35th Annual Meeting, NCR-84 Potato Breeding and Genetics Technical Committee, Chicago, Illinois, January 2005.<br /> <br /> Thompson, A.L., R.G. Novy, B.L. Farnsworth, G.A. Secor, N.C. Gudmestad, J.R. Sowokinos, E.T. Holm, J.H. Lorenzen, and D. Preston. 2005. Dakota Pearl: An attractive, bright white-skinned, cold-chipping cultivar with tablestock potential. Am. J. Potato Res. 82:481-488.<br /> <br /> Trabelsi, S., R. Garbouri-Bouzid, F. Vedel, A. Nato, L. Lakhoua, and N. Drira. 2005. Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei exhibit a recombination in the pastome. Plant Cell, Tissue & Organ Culture 83:1-11.<br /> <br /> Vaananen, T., T Ikonen, V.M. Rokka, P. Kuronen, R. Serimaa, and V. Ollilainen. 2005. Influence of incorporated wild Solanum genomes on potato properties in terms of starch nanostructure and glycoalkaloid content. J. Ag. Food Chem. 53:5313-5325.<br /> <br /> Veilleux, R.E. 2005. Cell and tissue culture of potato (Solanaceae). In: Genetic Improvement of Solanaceous Crops, Vol. I: Potato, M.K. Razdan and A.K. Mattoo (eds.), Science Publishers, Inc., Enfield, NH, pp. 184-208.<br /> Westermann, D.T. 2005. Nutritional requirements of potatoes. Am. J. Potato Res. 82:301-307.<br /> <br /> Theses Reporting Research with NRSP-6 Stocks<br /> <br /> Dinu, I.I. 2005. Introgression of wild Solanum species germplasm into cultivated potato gene pool using EBN manipulation. Ph.D. Dissertation, University of Minnesota, 138 pp.<br /> <br /> Sanchez, Maria J. 2005. Allelic mining for late blight resistance in wild Solanum species belonging to series Bulbocastana. M.S. Thesis, University of Minnesota, 180 pp.<br /> <br /> Villamon, Francisco. 2005. Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana). M.S. Thesis, University of Wisconsin-Madison.<br /> <br />

Impact Statements

1. NRSP-6s purpose is to provide a ready source of raw materials, technology and information to support potato enhancement, breeding and research in the USA and around the world. Thus, one way the success of NRSP-6 can be measured is by the occurrence of NRSP-6 germplasm in the pedigrees of new, improved potato cultivars. Another is in the use of NRSP-6 stocks in more basic research programs, which is reflected in publications that provide information that ultimately contribute to better exploitation of the germplasm resource.
2. Four cultivar releases were published in the American Journal of Potato Research in 2005: Dakota Pearl, Millenium Russet, Sierra Gold, and Summit Russet. Four verticillium wilt resistant germplasm releases were published in AJPR. All of these are known to have wild species in their pedigrees.
3. Section 6 lists 100 papers, 45 abstracts, and 3 theses which report the use of NRSP-6 Solanum introductions this year.

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Report Information

Participants

Abad, Jorge A. (jorge.a.abad@aphis.usda.gov) - USDA APHIS PPQ PGQP;
Ashworth, Edward (edward.ashworth@umit.maine.edu)- University of Maine;
Bamberg, John (John.Bamberg@ars.usda.gov)- USDA, ARS;
Bandla, Murali D. (murali.bandla@aphis.usda.gov) - USDA, APHIS, PPQ, PHP;
Bretting, Peter K. (peter.bretting@ars.usda.gov) - USDA, ARS;
Brown, Chuck R. (cbrown@pars.ars.usda.gov) - USDA, ARS;
De Jong, Walter (douchesd@msu.edu) - Cornell University;
Guthrie, Richard (guthrrl@auburn.edu) - Auburn University;
Haynes, Kathy (haynesk@ba.ars.usda.gov) - USDA, ARS - Vegetable Laboratories;
Hu, Ching Yuan (HuCY@ctahr.hawaii.edu) - University of Hawaii - Manoa;
Jahn, Molly M. (mjahn@cals.wisc.edu) - University of Wisconsin;
Martin, Max W. (mwmarti1@wisc.edu) - US Potato Genebank;
Miller, Jr., Creighton (jcmillerjr@tamu.edu) - Texas A&M University;
Palta, Jiwan (jppalta@wisc.edu) - University of Wisconsin;
de los Reyes, Benildo G (benildo.de@maine.edu) - University of Maine
Shafer, Steven R. (sshafer@mwa.ars.usda.gov) - USDA, ARS, MWA;
Simon, Philipp W. (psimon@wisc.edu) - USDA, ARS/University of Wisconsin;
Sklarczyk, Don (donsklarczyk@verizon.net) - Johannesburg, MI;
Spooner, David (dspooner@wisc.edu) - USDA, ARS/University of Wisconsin;
Tarn, T. Richard (tarnr@agr.gc.ca) - Agriculture and Agri-Food Canada;
Thill, Christian (thill005@tc.umn.edu) - University of Minnesota;
Thompson, Asunta Lee (asunta.thompson@ndsu.nodak.edu) - North Dakota State University;
Thro, Ann Marie (athro@csrees.usda.gov) - CSREES, USDA Plant & Animal Systems;
Vales, Isabel (isabel.vales@oregonstate.edu) - Oregon State University;
Veilleux, Richard (potato@vt.edu) - Virginia Polytechnic Institute;
Wisler, Gail C. (gail.wisler@ars.usda.gov) - GWCC-BLTSVL
Yencho, Craig (craig_yencho@ncsu.edu) - North Carolina State University

Brief Summary of Minutes

NRSP-6 TAC 2007 MINUTES


Peninsular Agricultural Research Station (PARS)
U.S. Potato Genebank (USPG)
Sturgeon Bay, WI
June 21, 2007


Creighton Miller, Chair, NRSP-6 TAC 07.
Chuck Brown, Vice Chair, NRSP-6 TAC 07 (absent)
David Douches, Secretary, NRSP-6 TAC 07 (absent); Isabel Vales acted as Secretary.


The meeting was called to order at 8:20 a.m.


Attendance


Attendance was poor this year due to budget constrains, travel cancellations and/or conflicts with other responsibilities.


Present: Ed Ashworth, John Bamberg, C. Y. Hu, Molly Jahn, Max Martin, Creighton Miller, Jiwan Palta, Phil Simon, Richard Tarn, Christian Thill, Gail Wisler, Isabel Vales


Absent: Chuck Brown, David Douches, Shelley Jansky, David Spooner, CSREES and APHIS not represented.


Morning Session


Preliminaries:
1. Welcome, introductions, announcements, distribution of documents
2. The 2007 agenda was distributed. No additions were made and the agenda was approved.
3. The 2006 minutes were distributed. Only a small modification was made: Learned that Hu is both Lead Administrative Advisor to NRSP-6 and Western Region Administrative Advisor. The minutes were approved as distributed. Isabel Vales moved acceptance and Creighton Miller seconded.
4. A Resolutions Committee was appointed by Miller, with by C.Y. Hu as Chair.


Pressing Issues:
- Interaction with CIP
o Getting materials was resolved
o APHIS/Quarantine problems have been resolved
- Personnel issues  quick overview
- Budgets. Where are we? What are the future plans?


Tour:
A tour of the USPG and PARS facilities (laboratories, greenhouse and field) started at 8:35 a.m. A handout with the names of staff members was distributed:
- Bamberg, ARS Lead Scientist and NRSP-6 Project Leader
- Martin, UW Academic Staff and NRSP-6 Project Assistant
- Stoneman, ARS Technician
- Schartner, ARS Technician
- Fernandez, UW Classified
- Del Rio, UW Academic Staff
- Temporary Workers and Students
- Associated Professional Staff:
- David Spooner: ARS Taxonomist, Collector and Herbarium Manager
- Shelley Jansky: ARS, germplasm evaluator and enhancer
- Phil Simon: ARS Research Leader
- Jiwan Palta: Formal Cooperative Agreement, research & U.WI Breeder


Afternoon Session
(Reports and Comments)


Coordination of Genebanks/Intergene Bank - John Bamberg
" In 1970s, CIP suggested cooperation.
" In the 1990s, linkages between banks were established, and there was a coordination of holdings. There has been little activity during the past few years.
" In 2005 there was a resurrection of global diversity.
" Collaborations continue with the CIP and German/Dutch potato collections. Some projects involve in situ work  collections and surveys are important.
" A cooperative agreement with CIP to have student exchanges would be good, and this is in the works.
" Important aspects related to coordination of Genebanks are to avoid duplication and to foster interaction and information sharing.

5/6. Report from Lead Administrative Advisor to NRSP-6  C.Y. Hu
" Hu will host the Western Regional meeting in Hawaii in March.
" Four centers hold germplasm in addition to NRSP-5 and NRSP-6
- Geneva, NY
- Experiment, GA
- Ames, IA
- Pullman, WA
" Funding for the four above mentioned centers is regional.
" NRSP-6 funding comes from the federal level. NRSP-6 funding is evaluated by the National Germplasm Review Committee. The recommendations are to restore funds at the 05-06 level ($150,000). Funding for NRSP6 is on the agenda for the July regional meeting. Funding depends on voting which will take place in September.


Ching Yuan Hu, Ph.D. (7/20/07)  written report:
The NRSP Review Committee met on June 6, 2007 in Kansas City to discuss NRSP budget proposals. Committee members present were Lee Sommers (CO), Chair and W rep; Marshall Martin (IN), NC rep; Bill Vinson (WV), NE rep; Craig Nessler (VA), S rep; Al Parks (Prairie View A&M), ARD rep; Larry Miller, CSREES rep; Eric Young, S Executive Director; Mike Harrington, W Executive Director; and , Don Latham (IA), Stakeholder rep.
During the Western Directors Joint Summer Meeting (July 16-18), Lee Sommers made a presentation on NRSP budget recommendations which the committee will be submitting to the Experiment Station Section at its September annual meeting in Philadelphia. The following is an excerpt from Lees report on NRSP6:
NRSP-6. Inter-Regional Potato Introduction Project. The amount requested for FY08 was $110,000. This project is an essential component of the National Plant Germplasm System, and the funding request is consistent with maintaining ongoing support from the SAES for the project. Motion by Nessler to accept budget request. Second by Latham. Motion passed.
I anticipate the NRSP Review Committee recommendations will be approved by the directors attending the ESS meeting. I spoke to Lee about NRSP6 budget request this morning. He indicated it is too late to make a request to change our budget proposal; but suggested us to submit a budget next year at the level of 2006 funding ($150K). I will work with John to prepare FY09 NRSP-6 budget proposal in January.


Issues to discuss: recover some costs (for example shipping and handling). Probably not necessary. Recipients pay for shipping if a fast delivery is requested. Shipping and handling costs will be difficult to manage through the USDA-ARS system.


Molly Jahn, Dean of the WI College of Agriculture, should be the Lead Administrative Advisor for this group, but since she is new to the group, Hu agreed to remain as the Lead AA for two more years.


11. Budget Report and Outlook - Bamberg
" From the Directors office: USDA does not have a budget for 2007.
" The presidents budget does not include special grants. Special grants CSREES disappeared, which was a major setback for participating states. $160 mill. moved into formula funds (Hatch funds)
" Now working on a new appropriation bill, farm bill
" Congress wants to move formula funds into multi-state competitive research.
" New initiative: CREATE 21 (Creative Research and Teaching)
" Special grants will not go away, but the Chair of the House Committee can reduce them. For next year, theyll reduce them to half. This will be discussed in September.


9. Additional Genebank Staff and Resources Report John Bamberg and Jiwan Palta
" Funding looks good. It will probably go back to the $150,000 level.
" The Dept. of Horticulture will reduce FTE by 1/3. The general feeling was that the interactions and synergy between USDA-ARS researchers and the university has been excellent, and it will be sad to see it decline due to budgetary issues.
" Some money was received at the end of the year and will continue at the same level.
" Annual report can be downloaded from the Web.

Introductions  stocks from the SW
B classification  Spooner was appointed to head collection and classification
Preservation  Virus PSTV
Part B. Evaluation
International collaborations
Nutrition
Tools  Germplasm, mutants
Alfonso del Rio  diversity impact
E. Distribution of materials: normal year
F. Visitors from China, from media
Useful findings  There are lots of potato varieties with exotic germplasm
Section 5. Continue
Section 6. Publications. Note: publications should be requested from collaborations/germplasm recipients
Set up parameters, metrics will be useful to reflect damage if funding is disrupted.


Formal Cooperative Agreements - Jiwan Palta
" Cooperative studies resulted in 2 NRI grants funded and training of several students.
" Research Topics: Cold tolerance and acclimation from biochemical and genetic perspectives
" Cooperative agreement under way to study GA and tuberization, tuber acidity, P, genetics of tuber calcium and carbosypeptidase acidity.
" Cooperation with CIP  trips


10. Collections and Taxonomy  John Bamberg
" CIP trips  able to collect again
" There are prospects about collecting in Peru.
" Engaging and showing benefit sharing are important


12/13. USDA, ARS Potato NPL  Gail Wisler
" Grants from USDA, ARS, and NPC resolved.
" The idea of expanding membership to include more breeders and the private sector was positively received.
" Potato cyst nematode discussion


8. Agriculture/Agrifood Canada. - Richard Tarn
" Richard is the PI for the potato breeding program.
" Participated in Canada Plant Resources Project. 132 accessions: 54 Canadian bred varieties. Working closely with seeds of diversity. They identified heirloom varieties of importance.
" Standard clones used in pathology as disease resistance checks
" Breeding lines of importance
" An annual newsletter is prepared. Check the web page Potato Research Center


GRINCA: Canadian version of GRIN
45 requests (511 units)
Most materials are in vitro
Personal information should not be disclosed
Official minutes should contain a synthesis of the reports
Technical reports are for internal communication and follow mission aspects


7. Regional and ARS Tech. Rep. Reports (posted on Genebank web site)
Western Region: Isabel Vales
Southern Region: Creighton Miller
North Central Region: Christian Thill
National Program Staff Report: Gail Wisler
Canada: Richard Tarn


Statistics
Report status of orders/research
Needs/advise


16. Review and Approve Resolutions. Chris Thill moved to approve and Tarn seconded. Motion passed.


17. Elect New Officers and Set next Meeting date
Chuck Brown, Vice Chair to Chair in 2008
David Douches, Secretary to Vice Chair in 2008
Isabel Vales, Acting Secretary to Secretary in 2008
Christian Thill moved to approve and Palta seconded. Motion passed.


2008 location: Cornell. Thill moved acceptance of meeting location and Gail seconded. Motion passed.


The meeting was adjourned at 6:02 p.m.


Resolutions:


Resolution #1.


WHEREAS, Dr. Steven Slack served as North Central Region Administrative Advisor and the Lead Administrative Advisor to the NRSP-6 Potato Germplasm Project; and


WHEREAS, he conscientiously provided liaison between the project and the USDA/NC Regional Association, as well as Experiment Station Section; therefore, be it


RESOLVED, that NRSP-6 TAC expresses highest appreciation for Dr. Slacks leadership and many contributions to the NRSP-6 and the development and use of potato species, and be it further


RESOLVED, that an original of this resolution be provided to Dr. Steven Slack, and that a copy be filed as part of the official minutes of this meeting.


Resolution #2.


WHEREAS, Dr. Steve Reiling served as Northeast Region Administrative Advisor to the NRSP-6 Potato Germplasm Project; and


WHEREAS, he conscientiously provided liaison between the project and the USDA/NE Regional Directors Association, be it


RESOLVED, that NRSP-6 TAC expresses highest appreciation for Dr. Reilings leadership and many contributions to the NRSP-6 and the development and use of potato species, and be it further


RESOLVED, that an original of this resolution be provided to Dr. Steve Reiling, and that a copy be filed as part of the official minutes of this meeting.


Resolution #3.


WHEREAS, the NRSP-6 TAC met at the Peninsular Agricultural Research Station, Sturgeon By, WI, June 21-22, 2007; and


WHEREAS, those attending were educated and stimulated by meetings and the station tour; and


WHEREAS, the location for the meeting was outstanding and the accommodations were both compatible and conducive to effective interaction resulting in a successful meeting; therefore, be it


RESOLVED, that the NRSP-6 TAC expresses its appreciation to Dr. John Bamberg and Mr. Max Martin for arranging the facilities and coordinating the meeting and the station tour, and be it further


RESOLVED, that an original of this resolution be provided to Dr. John Bamberg and that a copy be filed as part of the official minutes of this meeting.

Accomplishments

A. Introduction of New Stocks<br /> A total of 41 accessions were assigned PI numbers in 2006: one clone from Korea, one late blight clone from Mexico, one mapping population parent from CIP, 25 tuber moth resistant clones from CIP, and 13 accessions collected from Arizona. These accessions are now available from the NRSP-6 Solanum germplasm collection. <br /> <br /> <br /> The NRSP-6 web page (http://www.ars-grin.gov/nr6) has been updated to include all new stocks and screening information. Clientele that have ordered from NRSP-6 in the past four years are contacted three times per year informing them of new materials that are now available either as true seed, tubers, in vitro plantlets, or herbarium samples. A new service we are offering is dried ground leaf samples for DNA. For foreign requesters this is useful since there are no quarantine restrictions on dried material. <br /> <br /> <br /> B. Classification <br /> Dr. Spooner et al. have published and are working on five different areas of potato research: 1) molecular markers for genebank studies, 2) cultivated potato origins, 3) relationships in wild tomatoes and potatoes, 4) the predictive power of taxonomy relative to disease resistance data, and 5) linkage map of late blight in wild potatoes. <br /> <br /> <br /> C. Preservation and Increase of Stocks<br /> In 2006, a total of 223 accessions were increased as botanical seed populations.<br /> <br /> <br /> A total of 600 potato spindle tuber viroid (PSTVd) tests were performed on seed increase parents, seedlots and research materials. Germination tests were performed on 1,307 accessions, ploidy determinations were made on 40 accessions, and tetrazolium seed viability tests were done on 31 seedlots. <br /> <br /> <br /> <br /> D. Evaluation <br /> International collaboration. 1) with CIP, study of the impact of agrichemicals on in situ wild potato reproduction continued, and we initiated a related project to assess the impact of mining pollution on wild potato reproduction. The frost resistant breeding family developed by NRSP-6 staff was evaluated in Peru, and results appear to be promising. The elite clonal selections from this population grown in Hancock, Wisconsin had good tuber type and cold hardiness to 5oC. 2) with CIP, calcium application trials in the highlands showed impressive yield improvement, and suggest our parallel ongoing program to enhance calcium uptake efficiency via S. microdontum introgression might have impressive application in some locations in the Andes. 3) with CIP, progress was made on a joint project to study in situ germplasm capture (see below) and molecular-based assessment of allele density. 4) with PICTICPAPA: The entire collection of S. microdontum was evaluated for late blight in Toluca, Mexico (results pending). <br /> <br /> <br /> Nutritional. 1) Potato potassium is in a unique position to mitigate hypertension, which has huge health and economic impact. Potassium levels in the tubers are also correlated to the incidence of black spot bruise. We screened and found significant species differences in K uptake potential (funding anticipated from CGC). 2) Work continued with Dr. C. Millers lab screening for antioxidants in uncolored wild potato species tubers. We found an important GxE effect, demonstrating that some germplasm is only high antiox when grown artificially in the greenhouse, not in the field (two papers submitted). 3) Plans were made to preselect and screen the primitive cultivated species collection for high phenolics. 4) We began researching and planning experiments to examine anticancer potential of high tuber tomatine. 5) We characterized exotics and adapted materials for Potato Carboxypeptidase Inhibitor, a tiny heat- and digestion-resistant protein reported to inhibit some of the most pernicious human cancers (funded by the Crop Germplasm Committee, CGC). We staged parents for making and testing segregating hybrids for all of these traits. <br /> <br /> <br /> Mutants and other new traits. 1) We initiated crossing for adapted tubers in the background of the crazy sepal sterile floral mutant. BSA of crazy and flowering plants are planned for a fair test of the potential yield improvement without the sink in flowers. 2) Continued tests to relate tuber pH to other traits like calcium and antiox. S. microndontum was newly identified as a species for which some populations tubers are extremely acidic (10x most cultivated types). 3) Produced calcium introgression hybrids at 2x and 4x level and evaluated in WI and Hawaii. 4) Confirm that other GA mutants published as pito and ga2 are both likely the same as our ga1. Staged for study of spontaneous reversion to the functional allele. 5) Purple-less fendleri: Eliminated genome suppression as explanation for distorted segregation observed in some mutant testcrosses. <br /> <br /> <br /> Genebank technology and diversity management. 1) Found that long-term germination is declining more rapidly outside of local -20 and NSSL storage, so moved our active inventory to -20. 2) Found germination is not biased by density of sowing. 3) Found seedlots generated in 1986 with and without extra fertilization do not show clear germination effect due to seed quality after 20-yr storage. 4) Found seedlings are taller under sodium vs metal halide lamps. 5) Characterized 100 microdontum pops for pH, calcium, antiox, cs1 mutant and late blight pursuant to testing effectiveness of DNA-based core collection within a species. 6) Tested seed increase phenology and found later summer plantings produce more seeds per pollination. 7) With CIP cooperators continued case study of capture of diversity at easy-vs-hard collecting sites. 8) Found proximity of other species does not explain distinction of northern S. verrucosum populations in Mexico. 9) Published results showing small DNA differences between reputed duplicates in NRSP-6 and CIP collections. 10) Published conclusion that no loss of diversity happens at selection of excess small seedlings at transplant for inbred species.<br /> <br /> <br /> <br /> E. Distribution<br /> NRSP-6 distributions are shown in the table below. Stocks were sent to clientele in 25 states of the USA and 11 other countries. <br /> <br /> <br /> VOLUME AND TYPES OF STOCKS DISTRIBUTED<br /> <br /> <br /> <br /> Units1<br /> <br /> <br /> Category<br /> S<br /> TF<br /> TS<br /> IVS<br /> DNA<br /> PL<br /> HERB<br /> TOT<br /> PIs<br /> <br /> Domestic<br /> 3,794<br /> 214<br /> 1,158<br /> 617<br /> 0<br /> 11<br /> 0 <br /> 5,794<br /> 3,088<br /> <br /> Foreign<br /> 481<br /> 0<br /> 16 <br /> 20<br /> 42<br /> 0<br /> 0<br /> 559<br /> 529<br /> <br /> NRSP-62<br /> 9,192<br /> 0<br /> 62<br /> 284<br /> 0<br /> 0<br /> 0<br /> 9,538<br /> 2,271<br /> <br /> Total<br /> 13,467<br /> 214<br /> 1,236<br /> 921<br /> 42<br /> 11<br /> 0 <br /> 15,891<br /> 5,888<br /> <br /> <br /> 1 Types of stocks sent/(number of seeds, tubers or plantlets per standard shipping unit): S= True Seeds/(50), TF= Tuber Families/(10), <br /> <br /> TS=Tuber Stocks/(3), IVS=In Vitro Stocks/(1), DNA=DNA samples(1), PL=Plants in plugs/(1), Her= Herbarium/(1).<br /> <br /> 2 Includes chromosome counts, germination tests, ID and Taxonomic check plantings, in vitro maintenance, seed increases,<br /> <br /> PSTV tests, miscellaneous plantings, and NSSL seed backup.<br /> <br /> <br /> F. Visitors From Other Countries<br /> Dr. Shaoguan Duan-China; Dr. Liping Jin-China; Dr. Shuzhi Jaing-China; Dr. Kaiyun Xie-China; Dr. Wenbo Pan-China; Dr. Conghua Xie-China; Dr. Jun Liu-China; Dr. Nune Sarukhanyan-Armenia; Dr. Klaus J. Dehmer-Germany; Dr. Glenn Bryan-Scotland; Dr. Gavin Ramsay-Scotland; Dr. Caroline Marques Castro-Brazil<br /> <br />

Publications

<br /> <br /> Bamberg, John. 2006. Crazy Sepal: A new floral sepallata-like mutant in the wild potato Solanum microdontum Bitter. Am J Potato Res 83:433-435.<br /> <br /> <br /> <br /> Bamberg, J.B. and A. del Rio. 2006. Seedling transplant selection does not cause genetic shifts in genebank populations of inbred potato species. Crop Sci 46:424-427.<br /> <br /> <br /> <br /> Bamberg, John, Charles Fernandez and Alfonso del Rio. 2006. A new wild potato mutant in Solanum stoloniferum Schltdl. lacking purple pigment. Am J Potato Res 83:437-445.<br /> <br /> <br /> <br /> Bamberg, J., J.P. Palta and M. Martin. 2006. Using a wild species, S. microdontum, to move high calcium trait to the cultivated potatoes. In Potato Association of America/Solanaceae 2006 Annual Meeting. p. 163 (Abstract)<br /> <br /> <br /> <br /> Busse, J.S., J. Bamberg and J.P. Palta. 2006. Correlation between aerial and tuber calcium accumulation in Solanum genotypes segregating for tuber calcium uptake efficiency. In Potato Association of America/Solanaceae 2006 Annual Meeting. p. 204 (Abstract)<br /> <br /> Del Rio, A.H., J.B. Bamberg and Z. Huaman. 2006. Genetic equivalence of putative duplicate germplasm collections held at CIP and US Potato Genebanks. Am J Potato Res 83:279-285.<br /> <br /> Del Rio, Alfonso, John B. Bamberg and Charles Fernandez. 2006. Assessment of the genetic structure of in situ populations of wild potato Solanum fendleri eco-geographically dispersed in the Chiricahua mountains, Arizona. Am J Potato Res 83:108. (Abstract)<br /> <br /> Ghislain, M., D. Andrade, F. Rodriguez, R.J. Hijmans, and D.M. Spooner. 2006. Genetic analysis of the cultivated potato Solanum tuberosum L. Phureja Group using RAPDs and nuclear SSRs. Theor Appl Genet 113:1515-1527.<br /> <br /> Jansky, S.H., R. Simon and D.M. Spooner. 2006. Testing taxonomic predictivity. Crop Sci 46:2561-2570.<br /> <br /> Kuang, H.H., F.S. Wei, M.R. Marano, U. Wirtz, X.X. Wang, J. Liu, W.P. Shum, J. Zaborsky, L.J. Tallon, W. Rensink, S. Lobst, P.F. Zhang, C.E. Tornqvist, A. Tek, J. Bamberg, J. Helgeson, W. Fry, F. You, M.C. Luo, J.M. Jiang, C.R. Buell, and B. Baker. 2005. The R1 resistance gene cluster contains three groups of independently evolving, type I R1 homologues and shows substantial structural variation among haplotypes of Solanum demissum. Plant J 44(1):37-51. <br /> <br /> Lozoya-Saldana, H., O. Barrios and John Bamberg. 2006. Phytophthora infestans: races vs genotypes in the Toluca Valley, Mexico. Am J Potato Res 83:122. (Abstract)<br /> <br /> Nzaramba, Ndambe M., John Bamberg, Douglas C. Scheuring, and J. Creighton Miller Jr. 2006. Antioxidant activity in Solanum species as influenced by seed type and growing location. Am J Potato Res 83:127. (Abstract)<br /> <br /> Peralta, I.E., S. Knapp and D.M. Spooner. 2006. Nomenclature for wild and cultivated tomatoes. Rep Tomato Genet Coop 56:6-12.<br /> <br /> Spooner, D.M. and A. Salas. 2006. Structure, biosystematics, and genetic resources. In: J. Gopal and S.M. Paul Khurana (eds.) Handbook of potato production, improvement, and post-harvest management. Haworths Press, Inc., Binghampton, New York. Pgs.1-39.<br /> <br /> Vega, S.E., M. Aziz, J. Bamberg, A. Verma, and J.P. Palta. 2006. Screening potato germplasm for carboxy-peptidase inhibitor and its potential anticancer property. In Potato Association of America/Solanaceae 2006 Annual Meeting. p. 160 (Abstract) <br /> <br /> Vega, S.E., J.P. Palta and J. Bamberg. 2006. Exploiting cultivated germplasm to breed for enhanced tuber quality. In A.J. Bussan and M. Drilias (eds.). Proceedings of the Wisconsins Annual Potato Meeting. pp. 143-144. (Abstract)<br /> <br /> Vega, Sandra E., John B. Bamberg and Jiwan P. Palta. 2006. Gibberellin-deficient dwarfs in potato vary in exogenous GA3 response when the ga1 allele is in different genetic backgrounds. Am J Potato Res 83:357-363.<br /> <br /> Vega, Sandra E., Jiwan P. Palta and John B. Bamberg. 2006. Root zone calcium can modulate GA induced tuberization signal. Am J Potato Res 83:135. (Abstract) <br /> <br /> Vega, Sandra E., Jiwan P. Palta and John B. Bamberg. 2006. Exploiting cultivated germplasm to breed for enhanced tuber calcium accumulation ability. Am J Potato Res 83:136. (Abstract)<br /> <br />

Impact Statements

1. NRSP-6s purpose is to provide a ready source of raw materials, technology and information to support potato enhancement, breeding and research in the USA and around the world. Thus, one way the success of NRSP-6 can be measured is by the occurrence of NRSP-6 germplasm in the pedigrees of new, improved potato cultivars. Another is in the use of NRSP-6 stocks in more basic research programs, which is reflected in publications that provide information that ultimately contribute to better use of the germplasm resource.
2. Nine cultivar releases were published in the American Journal of Potato Research in 2006: Dakota Jewel, Dakota Rose, Beacon Chipper, Reeves Kingpin, Western Russet, Gemstar Russet, Marcy, Defender, and White Pearl. All of these are known to have wild species in their pedigrees.
3. 100 papers, 45 abstracts, and 4 theses which report the use of NRSP-6 Solanum introductions this year.

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Report Information

Participants

Martin, Max. W. (mwmarti1@wisc.edu) - NRSP6;
Hoopes, Bob (robert.hoopes@fritolay.com) - Frito Lay;
De Jong, Walter (wsd2@cornell.edu) - Cornell;
Bamberg, John (john.bamberg@ars.usda.gov) - USDA-ARS;
Kunibe, Elizabeth (elizbowe@hotmail.com) - USDA-SARU Univ. of AK;
Douches, David (douchesd@msu.edu) - Michigan State University;
Spooner, David (david.spooner@ars.usda.gov) - USDA-ARS;
Wisler, Gail (gail.wisler@ars.usda.gov) - USDA-ARS;
Abad, Jorge (Jorge.A.Abad@aphis.usda.gov) - USDA-APHIS;
Vales, Isabel (isabel.vales@oregonstate.edu) - Oregon State University;
Brown, Chuck (chuck.brown@ars.usda.gov) - USDA-ARS

Brief Summary of Minutes

NRSP-6 Technical Committee Meeting Minutes Synopsis


Venue: Best Western Columbia River Inn, Cascade Locks, OR / June 9-10, 2008

Leadership: C. Brown, Chair & Host; D. Douches, Vice Chair; I. Vales, Secretary

People present:
Martin, Max. W. (mwmarti1@wis.edu) - NRSP6;
Hoopes, Bob (robert.hoopes@fritolay.com) - Frito Lay;
De Jong, Walter (wsd2@cornell.edu) - Cornell;
Bamberg, John (john.bamberg@ars.usda.gov) - (USDA-ARS);
Kunibe, Elizabeth (elizbowe@hotmail.com) - USDA-SARU Univ. of AK;
Douches, David (douchesd@msu.edu) - Michigan State University;
Spooner, David (david.spooner@ars.usda.gov) - USDA-ARS;
Wisler, Gail (gail.wisler@ars.usda.gov) - USDA-ARS;
Abad, Jorge (jorge.a.abad@aphids.usda.gov) - USDA-APHIS;
Vales, Isabel (isabel.vales@oregonstate.edu) - Oregon State University;
Brown, Chuck (chuck.brown@ars.usda.gov) - USDA-ARS

Business:

Welcome, introductions, misc. announcements, distribution of documents were followed by a discussion of several related topics: Zebra chip, Purple top, nematodes, ethnobotany of potato in AK/PNW, SolCAP proposal. Review of 2007 minutes. No Admin Advisors were present. Reports were given by the five regional and ARS technical reps. Germplasm from NRSP6 continues to be extensively used to address practical potato breeding problems. No Agriculture and Agrifood Canada rep was present. Project Leader Bamberg gave report on follow-through for decisions made at the committee's 2007 meeting. Bamberg reported on advances in each of the five mission areas of the genebank: Introduction of new stocks, Preservation, Evaluation, Distribution (Classification would be later presented later by the project's taxonomist, Spooner). Bamberg presented budget news: Successful initiative to restore NRSP6 to $150K level and take our message directly to SAESD. Funding outlook from Wisconsin and ARS partners is not encouraging. Spooner presented results of recent taxonomic research and collecting. USDA/ARS/NPS news was represented by Wisler who reported on various national issues, technical and financial. No CSREES representative was present. Abad of APHIS/Quarantine Lab gave report on quarantine operations with respect to potato. Guest presentation on ethnobotany of native Alaskan potatoes was given by Kunibe. A resolution thanking the host was adopted, new officers were elected, and location for TAC09 selected. The NRSP6 meeting ended, and those present conducted business as the Potato Crop Germplasm committee meeting, conducting a preliminary discussion of FY09 germplasm evaluation grant proposals.

Accomplishments

3. PROGRESS AND PRINCIPAL ACCOMPLISHMENTS<br /> <br /> A. Introduction of New Stocks<br /> <br /> John Bamberg and Alfonso del Rio (US Potato Genebank) had a successful collecting expedition to the Guadalupe Mountain National Park (GMNP) in west Texas in late September of 2007 (supported with extramural funding from USDA). They collected four new populations. These were the first S. stoloniferum germplasm from Culberson County, TX and first potato collections from Eddy County, NM.<br /> <br /> A total of 5 accessions were assigned PI numbers in 2007: one primitive cultivar from Peru and 4 accessions collected from the SW United States. These accessions are now available from the NRSP-6 Solanum germplasm collection. <br /> <br /> The NRSP-6 web page (http://www.ars-grin.gov/nr6) has been updated to include all new stocks and screening information. Clients who have ordered from NRSP-6 in the past four years are contacted three times per year informing them of new materials that are now available either as true seed, tubers, in vitro plantlets, or herbarium samples. A new service we are offering is dried ground leaf samples for DNA testing. For foreign requesters this is useful since there are no quarantine restrictions on dried tissue. <br /> <br /> <br /> B. Preservation and Increase of Stocks<br /> <br /> In 2007, a total of 155 accessions were increased as botanical seed populations.<br /> <br /> A total of 672 potato spindle tuber viroid (PSTVd) tests were performed on seed increase parents, seedlots and research materials. Germination tests were performed on 1120 accessions, ploidy determinations were made on 35 accessions, and tetrazolium seed viability tests were done on 55 seedlots.<br /> <br /> Progress was made on several international collaborative projects. <br /> We are working with CIP on a project to assess the impact of agrichemicals on pollen and seed viability of wild species (oral presentation at the PAA07). Agrichemicals depress reproduction and diversity of wild potato germplasm, suggesting remote populations should be targeted for future collecting by genebanks.<br /> <br /> John Bamberg visited Peru in May 2007 to harvest calcium and frost plots. A low cost source of calcium applied to research plots located in the highlands of Peru resulted in an increase yield of 60% for some clones. Work is now being planned to identify genetics of stocks that respond well to added calcium. <br /> <br /> Frost is a major problem for the highland farmers in Peru, with major devastating episodes in the past two years. We supplied selected frost resistant germplasm for testing, and continue to synthesize hybrids of native cultivars with highly frost resistant wild species for tests in Peru.<br /> <br /> We initiated quarantine seed increase of seed rescued from the VIR collection to incorporate into the US Potato Genebank. We are also planning an intergenebank cooperative project to evaluate primitive cultivated species andigena for hidden recessive traits and incorporate word holdings data into the global germplasm database (IPD).<br /> <br /> Hypertension in humans and black spot bruise in potatoes are both problems for which increased tuber potassium could have a positive influence. Potassium screening of the minicore collection funded by the Crop Germplasm Committee (CGC) grant has shown great species variation which could lead to a selection of high-K varieties that lower blood pressure and reduce black spot bruise.<br /> <br /> We continued screening for antioxidants in uncolored wild potato species tubers of S. okadae hybrids with USDA cooperator Dr. Navarre. We have extended this work to discovery of high antiox species more crossable to tuberosum, and synthesis of diploid segregating populations. <br /> <br /> We discovered a new floral development mutant in S. microdontum which we have named crazy sepal (cs1) because it grows multiple (indeterminate) sets of sepals instead of anthers and pistils. This is a potential tool for studying floral development and cost of flowering on tuber yield. It would prevent transgene escape from GMO potato. We have made crosses with diploid tuberosum to investigate these opportunities and made the mutant available to other workers (Short Communication in AJPR).<br /> <br /> Plant parts are known to vary widely for pH, but little is known about the variation, basis, or utility of pH extremes in potato tubers. When pH was measured on species of the mini-core collection, replicates over years and populations gave consistent results, with pH range of (5.0-6.2). Fine screening has now discovered very acidic species more amenable to crossing with tuberosum, and we have produced F2 segregating populations. This opens the door to studies linking acidity to a wide range of tuber parameters like processing quality, skin color retention, disease resistance, calcium content, glycemic index and acrylamide formation. <br /> <br /> Solanum microdontum is remarkably extreme and variable for several characteristics (acid, potassium, calcium, late blight and antioxidants). We continued to perform replicated characterization of S. microdontum for these traits. We also collected DNA to compare variation for useful phenotypic traits to gross genetic variation.<br /> <br /> C. Classification <br /> <br /> Dr. Spooner et al. have published and are working on five different areas of potato research: 1) molecular markers for genebank studies, 2) cultivated potato origins, 3) relationships in wild tomatoes and potatoes, 4) the predictive power of taxonomy relative to disease resistance data, and 5) a linkage map showing late blight in wild potatoes. <br /> <br /> <br /> D. Distribution<br /> <br /> The volume and types of stocks sent to various consignee categories are summarized in the table below. NRSP-6 distributed to clients in 24 states of the USA and 8 other countries. <br />

Publications

6. PUBLICATIONS ISSUED DURING THE YEAR<br /> <br /> A. Publications issued by NRSP-6 Personnel<br /> <br /> Alvarez, N.M., I.E. Peralta and D. Spooner. 2007. Morphological evaluation of the Solanum brevicaule complex: a replicated field trial from Argentina. Am J Potato Res 84:73-74. (Abstract)<br /> <br /> Ames, M., A. Salas-Lopez and D. Spooner. 2007. Taxonomic evaluation of putatively related wild potato species of Solanum series Cuneoalata, Ingifolia, Olmosiana, Piurana, and Simplicissima, by morphological data from an Andean field station. Am J Potato Res 84:74. (Abstract)<br /> <br /> Bamberg, J.B. 2007. Crazy Sepal: A new floral Sepallata-like mutant in the wild potato Solanum microdontum Bitter. Am J Potato Res 84:76. (Abstract)<br /> <br /> Bamberg, J.B., A. del Rio and C. Fernandez. 2007. Sampling remote in situ sites of USA wild potato captures more diversity. Am J Potato Res 84:76. (Abstract)<br /> <br /> Bamberg, John and Alecia Kiszonas. 2007. Variation for tuber acidity among potato species. Am J Potato Res 84:76-77. (Abstract)<br /> <br /> Bamberg, J.B., J.P. Palta and M.W. Martin. 2007. Using a wild species, Solanum microdontum, to move high tuber calcium trait to the cultivated potatoes. Am J Potato Res 84:77. (Abstract)<br /> <br /> Busse, J.S., J.B. Bamberg and J.P. Palta. 2007. Correlation between aerial shoot and tuber calcium accumulation in Solanum genotypes segregating for tuber calcium uptake efficiency. Am J Potato Res 84:79-80. (Abstract)<br /> <br /> Centeno-Diaz, Ruth, Alberto Salas-Lopez, Alfonso del Rio, John Bamberg, and William Roca. 2007. Impact of crop pesticides on the reproductive ability of wild potato species. Am J Potato Res 84:81-82. (Abstract)<br /> <br /> Fajardo, D., A. Salas-Lopez, R. Castillo, and D. Spooner. 2007. Species and series boundaries of Solanum series Conicibaccata and phonetically similar species in ser. Piurana (sect. Petota): Morphological data from a field study in Peru. Am J Potato Res 84:89-90. (Abstract)<br /> <br /> Hijmans, R.J., T. Gavrilenko, S. Stephenson, J.B. Bamberg, A. Salas, and D.M. Spooner. 2007. Geographical and environmental range expansion through polyploidy in wild potatoes (Solanum section Petota). Global Ecol Biogeogr 16:485-495 (Supplement 1, Supplement 3, Ploidy paper data)<br /> <br /> Knapp, S., D.M. Spooner and B. Leon. 2006. Solanaceae endemicas del Peru. Rev. Peru. Biol. Numero especial 13(2):612s-643s.<br /> <br /> Nzaramba, M. Ndambe, J.B. Bamberg and J.C. Miller, Jr. 2007. Effect of propagule type and growing environment on antioxidant activity and total phenolic content in potato germplasm. Am J Potato Res 84:323-330.<br /> <br /> Peralta, I.E. and D.M. Spooner. 2007. History, origin and early cultivation of tomato (Solanaceae). Pp 1-27. In: Genetic Improvement of Solanaceous Crops, Vol. 2: Tomato. M.K. Razdan and A.K. Mattoo (eds.), Science Publishers, Enfield, USA.<br /> <br /> Rios, D., M. Ghislain, F. Rodriguez, and D.M. Spooner. 2007. What is the origin of the European potato? Evidence from Canary Island landraces. Crop Sci 47:1271-1280.<br /> <br /> Rodriquez, F., F. Wu, S. Tanksley, and D. Spooner. 2007. A multiple single-copy gene phylogenetic analysis of wild tomatoes (Solanum L. section Lycopersicon (Mill.) Wettst.) and their outgroup relatives. Am J. Potato Res 84:113. (Abstract)<br /> <br /> Salas-Lopez, A., N.M. Alvarez, M. Ames, M. Blancas, D. Fajardo, H. Juarez, I.E. Peralta, W. Roca, E. Rojas, R. Simon, M. Vargas, K. Vivanco, and D. Spooner. Am J Potato Res 84:115. (Abstract)<br /> <br /> Simko, I., S.H. Jansky, S. Stephenson, and D.M. Spooner. 2007. Genetics of resistance to pests and disease. In: Potato Biology and Biotechnology: Advances and Perspectives. R. Vreugdenhil, J. Bradshaw, C. Gebhardt, F. Govers, D.K.L. MacKerron, M.A. Taylor, and H.A. Ross (eds.). pp. 117-155, Elsevier, Amsterdam, The Netherlands.<br /> <br /> Spooner, D.M. 2006. Simsia Persoon for Flora of North America North of Mexico. Vol. 21:140-141. Oxford University Press, New York.<br /> <br /> Spooner, D.M., L. Bohs, J. Giovannoni, R. Olmstead, and S. Daitsuke (Editors). 2007. Solanaceae VI  Genomics meets biodiversity. Acta Hort 1-564.<br /> <br /> Spooner, D.M., D. Fajardo and G.J. Bryan. 2007. Species limits of Solanum berthaultii Hawkes and S. tarijense Hawkes and the implications for species boundaries in Solanum sect. Petota. Taxon 56:987-999.<br /> <br /> Spooner, D.M., S.H. Jansky and A.J. Bussan. 2007. Experiences of a local arrangement committee for a large scientific conference. Acta Hort 745:513-532.<br /> <br /> Spooner, D.M., J. Nunez, G. Trujillo, M. del Rosario Herrera, F. Guzman, and M. Ghislain. 2007. Extensive simple sequence repeat genotyping of potato landraces supports a major reevaluation of their gene pool structure and classification. Proc Natl Acad Sci USA 104:19398-19403.<br /> <br /> Vega, S., M. Aziz, J. Bamberg, A. Verma, and J. Palta. 2007. Screening potato germplasm for carboxypeptidase inhibitor and its potential anticancer properties. Am J Potato Res 84:118-119. (Abstract)<br /> <br /> B. Journal Articles and Abstracts Reporting Research with NRSP-6 Stocks<br /> <br /> Alvarez, A.E. 2007. Resistance mechanisms of Solanum species to Myzus persicae. Wageningen University, Wageningen Netherlands. Iv + 182 pp.<br /> <br /> Alvarez, A.E., W.F. Tjallingii, E. Garzo, V. Vleeshouwers, M. Dicke, and B. Vosman. 2006. Location of resistance factors in the leaves of potato and wild tuber-bearing Solanum species to the aphid Myzus persicae. Ent Exp et Appl 121(2):145-157.<br /> <br /> Bae, Jinjoo, Shelley Jansky and Douglas Rouse. 2007. Early generation selection for resistance to Verticillium wilt. Am J Potato Res 84:75. (Abstract)<br /> <br /> Baldwin, S., R. Genet, R. Macknight, K. Dodds, and J.M.E. Jacobs. 2007. Genetics of resistance to powdery scab (Spongospora subterranean) of potato. Am J Potato Res 84:75-76. (Abstract)<br /> <br /> Beketova, M.P., P.E. Drobyazina and E.E. Khavkin. 2006. The R1 gene for late blight resistance in early and late maturing potato cultivars. Russian j Plant Physiol 53(3):384-389.<br /> <br /> Beketova, M.P., P.E. Drobyazina, E.A. Sokolova, E.A. Tsarkova, V.A. Vorobiev, I.M. Yashina, and E.E. Khavkin. 2007. DNA markers for potato introgression breeding, In: Potato production and innovative technologies (Haverkort, A.J. and Anisimov, B.V., eds.), Wageningen Acad. Publ., pp. 397-404.<br /> <br /> Belknap, W.R., S.S. Chang, D.R. Rockhold, N.T. Taylor, and K.F. McCue. 2007. Solanum bulbocastanum polyubiquitin Bu427 promoter and uses thereof. Patent Pending: United States.<br /> <br /> Belknap, W.R., S.S. Chang, D.R. Rockhold, N.T. Taylor, and K.F. McCue. 2007. Solanum bulbocastanum polyubiquitin Bul409 promoter and uses thereof. Patent Pending: United States.<br /> <br /> Benoit, Bizimungu and Qin Chen. 2007. Evaluation of interspecific hybrid derivatives possessing disease resistance traits for potential use in a potato breeding program. Am J Potato Res 84:78. (Abstract)<br /> <br /> Bernal, A.M., J.E. Arias, J.D. Moreno, I. Valbuena, and L.E. Rodriguez. 2006. Confrontation between an a priori clustering of potato germplasm Solanum tuberosum subspecies andigena vs a non-hierarchic clustering. Agronomia Colombiana 24(2):214-225.<br /> <br /> Bizimungu, B., D.R. Lynch, L.M. Kawchuk, Q. Chen, M. Konschuh, C. Schaupmeyer, J. Wahab, D. Waterer, D. Driedger, H. Wolfe, P. McAllister, R. Howard, and H. W. Platt. 2007. Northstar: A high-yielding, white cold-storage chipping potato cultivar with attractive oval tubers resistant to late blight. Am J Potato Res 84:437-445.<br /> <br /> Bradeen, J.M. and D.S. Mollov. 2007. Herbicide tolerance in primitive diploid potato species comprising superseries Stellata: Toward establishment of seedling cultivation conditions for field evaluations. Am J Potato Res 84:415-424.<br /> <br /> Bradeen, J.M. and D.S. Mollov. 2007. Herbicide tolerance in Diploid 1EBN and 2EBN potato species. Potato Assoc of America Annual Meeting, Idaho Falls, ID, Aug. 2007. (Abstract)<br /> <br /> Burkhart, C.R., B.J. Christ and K.G. Haynes. 2007. Non-additive genetic variance governs resistance to Fusarium dry rot in a diploid hybrid potato population. Am J Potato Res 84:199-204.<br /> <br /> Carputo, D., L. Castaldi, I. Caruso, R. Aversano, L. Monti, and L. Frusciante. 2007. Resistance to frost and tuber soft rot in near-pentaploid Solanum tuberosum-S. commersonii hybrids. Breed Sci 57(2):145-151.<br /> <br /> Carputo, Domenico, Amalia Barone, Immacolata Caruso, Massimo Iorizzo, Astolfo Zoina, and Luigi Frusciante. 2007. Fertile Solanum commersonii-S. tuberosum sexual hybrids as source of resistance to Ralstonia solanacearum. Am J Potato Res 84:80. (Abstract)<br /> <br /> Chantha, S.-C. and D.P. Matton. 2007. Underexpression of the plant NOTCHLESS gene, encoding a WD repeat protein, causes pleitropic phenotype during plant development. Planta 225:1107-1120.<br /> <br /> Chantha, S.-C., F. Tebbji and D.P. Matton. 2007. From the Notch signaling pathway to ribosome biogenesis. Plant Signaling & Behavior 2:168-170.<br /> <br /> Chevalier, E. and D.P. Matton. 2007. RALF, a secreted peptide involved in plant reproduction. Plant Biology & Botany Joint Congress, American Society of Plant Biologists (ASPB), Chicago, Illinois, USA, 7-11 July, 2007. Poster presentation. (Abstract)<br /> <br /> Clark, Catherine, Yvan Pelletier, Robert Coffin, and William Hardy. 2007. Field evaluation of resistance of wild Solanum species to aphids. Northeast Potato Technology Forum. Charlottetown, PEI, Canada. March 14-15, 2007.<br /> <br /> Colton, L., S. Wielgus, Y. Chen, B. Bowen, F. Nevaro, and J. Jiang. 2007. Continuation of marker-assisted selection for late blight resistant potatoes. Am J Potato Res 84:83-84. (Abstract)<br /> <br /> Cortes, C., E. Salazar, H. Prieto, and R. Pertuze. 2006. Determinacion del grado de hibridacion entre papa cultivada (Solanum tuberosum) y especies silvestres relacionadas a partir de polinizacion dirigida (Determination of the degree of hibridization among cultivated potato (Solanum tuberosum) and wild relative species by manual pollinization). 57° Congreso Agronomico de Chile, Santiago-Chile, October 17-20, 2006. Abstract book, p. 97. (Abstract)<br /> <br /> Cortes, C., E. Salazar, H. Prieto, and R. Pertuze. 2007. Antecedentes sobre la Posibilidad de Hibridacion entre Papa Cultivada y Diez Especies de Solanum Silvestres Nativos de Chile (Antecedents about hybridization possibility between cultivated potato and ten wild native Solanum species of Chile). VI Symposium of Genetic Resources for Latin America and the Caribbean. Mexico DF, Mexico. November 13-16, 2007. (Abstract)<br /> <br /> Costanzo, S., M. Ospina-Giraldo, K.L. Deahl, C.J. Baker, and R.W. Jones. 2007. Alternate intron processing of family 5 endoglucanase transcripts from the genus Phytophthora. Current Genetics 52:115-123.<br /> <br /> DHoop, B.B., M.J. Paulo, R.A. Mank, H.J. van Eck, and F.A. van Eeuwijk. 2007. Association mapping of quality traits in potato (Solanum tuberosum L.). Euphytica DOI 10.1007/s10681-007-9565-5.<br /> <br /> Domanski, L., E. Zimnoch-Guzowska, M. Domanska, and H. Jakuczun. 2006. The effect of heterosis of diploid potato clone DG.97-943 of cold shipping type and producing 2n gametes. Biuletyn Instyt Hod Aklim Roslin 240/241:307-313.Gray-Mitsumune, M., M. OBrien, C. Bertrand, F. Tebbji, A. Nantel, and D.P. Matton. 2006. Loss of ovule identity induced by over-expression of the fertilization-related kinase 2 (ScFRK2), a MAPKKK FROM Solanum chacoense. J Exp Botany 57(15):4171-4187.<br /> <br /> Dorion, S., F. Dumas and J. Rivoal. 2006. Autophosphorylation of Solanum chacoense cytosolic nucleoside diphosphate kinase on Ser117. J Exp Botany 57(15):4079-4088.<br /> <br /> Drobyazina, P.E. and E.E. Khavkin. 2007. Structural homologs of CONSTANS and LEAFY in potato and its wild relatives. Acta Hort 745:411-419.<br /> <br /> Drobyazina, P.E. and E.E. Khavkin. 2007. Two structural variants of the CONSTANS-LIKE gene in long- and short-day Solanum plants. ASPB Plant Biology & Botany Joint Congress, Chicago, USA, July 2007. (Abstract)<br /> <br /> Drobyazina, P.E., E.V. Rogozina, E.A. Sokolova, E.A. Tsarkova, V.A. Vorobiev, I.M. Yashina, G.A. Yakovleva, and E.E. Khavkin. 2007. SCAR-markers of Solanum wild species for introgression potato breeding. II International Vavilov Conference Crop Genetic Resources in the 21st Century: Current Status, Problems and Prospects, St. Petersburg, Russia, November 2007. (Abstract)<br /> <br /> Erazzu, L.E. and E.L. Camadro. 2007. Direct and indirect detection of 2n eggs in hybrid diploid families derived from haploid tbr x wild species crosses. Euphytica 155(1/2):57-62.<br /> <br /> Franco J. and G. Main. 2006. Screening for resistance to Nacobbus aberrans and Globodera spp. in wild potato species resistant to other pathogens. Nematol Mediterranea 34(2):165-169.<br /> <br /> Gabriel, J., A. Coca, G. Plata, and J.E. Parlevliet. 2007. Characterization of the resistance to Phytophthora infestans in local potato cultivars in Bolivia. Euphytica 153(3):321-328.<br /> <br /> Germain, H., J. Houde, M. Gray-Mitsumune, R.T. Sawasaki, Y. Endo, and D.P. Matton. 2007. Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense. FEBS Letters 581:5137-5142.<br /> <br /> Gillen, A.M. and R.G. Novy. 2007. Molecular characterization of the progeny of Solanum etuberosum identifies a genomic region associated with resistance to potato leafroll virus. Euphytica 155(3):403-415.<br /> <br /> Goyer, A. and D.A. Navarre. 2007. Determination of folate concentrations in diverse potato germplasm using a trienzyme extraction and a microbiological assay. J Ag Food Chem 55(9):3523-3528.<br /> <br /> Goyer, A., P. Hamm and R. Navarre. 2007. Germplasm mining and metabolic engineering as strategies to boost folates in potato tubers. Am J Potato Res 84:90-91. (Abstract)<br /> <br /> Groza, H.I., B.D. Bowen, A.J. Bussan, W.R. Stevenson, F. Navarro, D. Kichefski, S.J. Peloquin, J. Palta, and J. Jiang. 2007. MegaChip  A new potato variety for chipping. Am J Potato Res 84:343-350.<br /> <br /> Halseth, D.E. and E.R. Sandsted. 2007. Management profile for Marcy, a high yielding chipping variety. Am J Potato Res 84:91. (Abstract)<br /> <br /> Haynes, K.G. 2007. Chip color in a high specific gravity Solanum phureja-Solanum stenotomum population. Am J Potato Res 84:92. (Abstract)<br /> <br /> Haynes, K.G., R.W. Goth, D.H. Lambert, and B.J. Christ. 2007. Evaluation of a short-day adapted tetraploid potato population with horizontal resistance to Phytophthora infestans under long-day conditions in northern Maine. Am J Potato Res 84:459-466.<br /> <br /> Honermeier, B. and G. Knipp. 2006. Fructans potato  health improving components in new tubers. Kartoffelbau 7:336-338.<br /> <br /> Hudak, I., J. Dobranszki and M. Hevesi. 2006. In vitro methods for testing potato clones against soft rot Erwiniae. In: Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding. Debrecen, Hungary, September 12-17, 2004. (M.G. Fari, I. Holb and G.D. Bisztray, eds.) Acta Hort 725(1):445-449.<br /> <br /> Inglis, D.A., C.R. Brown, B.G. Gunderson, L.D. Porter, J.S. Miller, D.A. Johnson, H. Lozoya-Saldana, and K.G. Haynes. 2007. Assessment of Solanum hougasii in Washington and Mexico as a source of resistance to late blight. Am J Potato Res 84:217-228.<br /> <br /> Iorizzo, M., J.M. Bradeen, L. Frusciante, and D. Carputo. 2007. Development of a DarT Microarray for Comparative Structural Genomics and Mapping of Agriculturally Significant Genes in Wild Potato Species. ANNUAL CONGRESS SOCIETA ITALIANA DI GENETICA AGRARIA XLXI, Riva del Garda, Italy, Sept. 2007. (Abstract)<br /> <br /> Iovene, M., S. Savarese, T. Cardi, L. Frusciante, N. Scotti, P. Simon, and D. Carputo. 2007. Assessment of nuclear and cytoplasmic genome composition in Solanum bulbocastanum (+) S. tuberosum somatic hybrids. Am J Potato Res 84:95. (Abstract)<br /> <br /> Iovene, M., S. Savarese, T. Cardi, L. Frusciante, N. Scotti, P.W. Simon, and D. Carputo. 2007. Nuclear and cytoplasmic genome composition of Solanum bulbocastanum (+) S. tuberosum somatic hybrids. Genome 50(5):443-450.<br /> <br /> Jansky, Shelley. 2007. Characterization of genotypic and environmental contributions to baked potato flavor. Am J Potato Res 84:95-96. (Abstract)<br /> <br /> Kelly, K., A. Gillen, J. Whitworth, and R. Novy. 2007. Development of molecular markers for introgression of potato leafroll virus resistance from S. etuberosum into cultivated potato. Am J Potato Res 84:97. (Abstract)<br /> <br /> Khu DongMan, S.L. Love, Mo HwangSung, Li KuiHua, and Lim HakTae. 2007. In vitro screening of potato genotypes for resistance to common scab using thaxtomin A. Hort Environ Biotech 48(4):224-227.<br /> <br /> Kielbowicz-Matuk, Agnieszka and Tadeusz Rorat. 2007. Expression of gene encoding lipid transfer protein (SsLTP1) is associated with adaptation of S. sogarandinum to dehydration stresses (cold, drought and salinity). XV Ogolnopolskie Seminarium Sekcji Mrozoodpornosci, KNO PAN, Iinstytut Dendrologii PAN w Korniku, 24-25 maj 2007, -str. 5. (Abstract)<br /> <br /> Kielbowicz-Matuk, Agnieszka, Pascal Rey and Tadeusz Rorat. 2007. The abundance of a single domain cyclophilin in Solanaceae is regulated as a function of organ type and high temperature and not by other environmental constraints. Physiol Plant 131:387-398.<br /> <br /> Kozlov, V., N. Rusetsky, A. Chashinsky, H. Zhuromski, and A. Rajko. 2007. Wild species of Potato, as sources of resistance to Pathogens. Plant protection: Manual of Proceeding/Anniversary of the Institute of Plant Protection  Nesvizh, 2007. <br /> Pp 196-203.<br /> <br /> Kozlov, V.A., N.V. Rusetsky and A.V. Chashinsky. 2007. Studying and use of wild and cultural kinds at creation of an initial material of a potato//Genetic resources of cultural plants in XXI century: a condition, problems, prospects / Theses of reports II Vavilovs conferences. St. Petersburg, November 26-30, 2007, St. Petersburg, VIR, 2007. C. 483-485.<br /> <br /> Kozlov, V.A., N.V. Rusetsky and A.V. Chashinsky. 2007. Results of selecting initial breeding material for potato//Potato growing: Manual of Proceeding/Research and Practical Center of National Academy of Sciences of Belarus for Potato, Fruit and Vegetable Growing. Minsk, 2007, 12:153-165.<br /> <br /> Lafleur, E., C. Kapfer, M. Gray-Mitsumune, and D.P. Matton. 2007. Involvement of the Solanum chacoense MAPKKK SFRK1 in embryo sac development. Plant Biology & Botany Joint Congress, American Society of Plant Biologists (ASPB), Chicago, Illinois, USA, 7-11 July, 2007. Oral presentation. (Abstract)<br /> <br /> Lafleur, E., C. Kapfer, M. Gray-Mitsumune, and D.P. Matton. 2007. A family of MAPKKK from Solanum chacoense involved in ovule and embryo sac development. Plant Protein Phosphorylation-Dephosphorylation & Mini-Symposium on Plant Proteomics, Twenty-Fourth Annual Missouri Plant Biology Symposium, May 22-25, 2007, C.S. Bond Life Sciences Center, University of Missouri, Columbia, Missouri, USA. Poster presentation. (Abstract)<br /> <br /> Lawrence, S.D., N.G. Novak and M.B. Blackburn. 2007. Inhibition of proteinase inhibitor transcripts by Leptinotarsa decemlineata regurgitant in Solanum Lycopersicum. J Chem Ecol 33(5):1041-1048.<br /> <br /> Levy, D. and R.E. Veilleux. 2007. Adaptation of potato to high temperatures and salinity  a review. Am J Potato Res 84:487-506. (Invited Review)<br /> <br /> Li, Haiyan, D. Beasley and Q Chen. 2007. Cytological and RAPD analysis of interspecific somatic hybrids Solanum pinnatisectum (+) S. tuberosum. Am J Potato Res 84:100-101. (Abstract)<br /> <br /> Lightbourn, G.J., J.G. Jelesko and R.E. Veilleux. 2007. Retrotransposon-based markers from potato monoploids used in somatic hybridization. Genome 50:492-501.<br /> <br /> Lightbourn, G.J. and R.E. Veilleux. 2007. Production and evaluation of somatic hybrids derived from monoploid potato. Am J Potato Res 84:425-435.<br /> <br /> Liu, Z.Y. and D. Halterman. 2006. Identification and characterization of RB-orthologous genes from the late blight-resistant wild potato species Solanum verrucosum. Physiol Molec Pl Path 69(4/6):230-239.<br /> <br /> Liu, Zhenyu and Dennis Halterman. 2007. Proteomic analysis of potato late blight resistance mediated by the RB resistance gene. Am J Potato Res 84:101. (Abstract)<br /> <br /> Luczak, Wojciech and Tadeusz Rorat. 2007. Expression of gene encoding cysteine protease (CysP) is induced in Solanum sogarandinum in response to drought. XV Ogolnopolskie Seminarium Sekcji Mrozoodpornosci, KNO PAN, Iinstytut Dendrologii PAN w Korniku, 24-25 maj 2007, -str. 6. (Abstract)<br /> <br /> Marcellan, O.N., A. Acevedo and E.L. Camadro. 2006. S16, a novel S-RNase allele in the diploid species Solanum chacoense. Genome 49(8):1052-1054.<br /> <br /> Matton, D.P. 2007. Rôles dun famille de MAPKKK et de récepteurs kinases lors de la reproduction sexuée chez les plantes. Département de Biologie, Séminaires du Centre SÈVE, Université de Sherbrooke, Sherbrooke, Canada, 12 December 2007. Invited seminar speaker. Seminar given in French. (Abstract)<br /> <br /> Matton, D.P. 2007. Functional genomics of signal transduction pathways during reproduction in a Solanaceous species: involvement of MAPK cascades and of receptor-like kinases. Technische Universität München, Wissenschaftszentrum Weihenstephan, Freising, Germany, 15 November 2007. Invited seminar speaker. (Abstract)<br /> <br /> McCann, Leah and Philipp Simon. 2007. Reducing sugar, asparagine, and chip color in wild Solanum species. Am J Potato Res 84:103. (Abstract)<br /> <br /> McCue, K.F., P.V. Allen, L.V. Shepherd, A. Blake, M.M. Maccree, D.R. Rockhold, R.G. Novy, D. Stewart, H.V. Davies, and W.R. Belknap. 2007. Potato glycosterol rhamnosyltransferase, the terminal step in triose side-chain biosynthesis. Phytochem 68:327-334.<br /> <br /> McCue, K.F., P.V. Allen, L.V. Shepherd, A. Blake, D.R. Rockhold, R.G. Novy, D. Stewart, H.V. Davies, and W.R. Belknap. 2007. Manipulation and compensation of steroidal glycoalkaloid biosynthesis in potatoes. Acta Hort 745:343-350.<br /> <br /> McCue, K.F., P.V. Allen, L.V. Shepherd, D.R. Rockhold, M.M. Maccree, H.V. Davies, and W.R. Belknap. 2007. Solanum tuberosum sterol alkaloid glycosyltransferase a novel solanidine glucosyltransferase SGT2 and uses thereof. Patent Allowed.<br /> <br /> Millett, B.P. and J.M. Bradeen. 2007. Development of allele-specific PCR and RT-PCR assays for clustered resistance genes using a potato late blight resistance transgene as a model. Theor Appl Genet 114:501-513.<br /> <br /> Millett, B.P. and J.M. Bradeen. 2007. Effects of foliar blight resistance transgene RB on tuber blight resistance. Plant & Animal Genome XV, San Diego, CA, Jan. 2007. (Abstract)<br /> <br /> Mizubuti, E.S.G. and W.E. Fry. 2006. Potato late blight. In: The epidemiology of plant diseases (B.M. Cooke, D.G. Jones and B. Kaye, eds.). Dordrecht, Netherlands; Springer SBM (2006) Ed. 2:445-471.<br /> <br /> Mojtahedi, H., C.R. Brown, E. Riga, and L.H. Zhang. 2007. A new pathotype of Meloidogyne chitwoodi Race 1 from Washington State. Plant Disease 91(8):1051.<br /> <br /> Mollov, D., M. Iorizzo, S. Wielgus, J. Raasch, D. Carputo, L. Frusciante, S. Austin-Phillips, J. Jiang, and J.M. Bradeen. 2007. The transgene RB renders foliar late blight susceptible potato cultivars resistant. Potato Assoc of America Annual Meeting, Idaho Falls, ID, Aug. 2007. (Abstract)<br /> <br /> Nath, S.K. Abul Khair and M.R. Ali. 2006. Screening of potato germplasm against root knot (Meloidogyne incognita, M. javanica), and lesion nematodes (Pratylenchus spp.). Bangladesh J Pl Path 22(1/2):63-67.<br /> <br /> Navarre, Roy, Roshani Shakya, Aymeric Goyer, Joanne Holden, and Richard Novy. 2007. Germplasm mining in potato to enhance nutritional quality and disease resistance. Am J Potato Res 84:106. (Abstract)<br /> <br /> Nouri-Ellouz, O., R. Gargouri-Bouzid, D. Sihachakr, M.A. Triki, G. Ducreux, N. Drira, and L. Lakhoua. 2006. Production of potato intraspecific somatic hybrids with improved tolerance to PVY and Pythium aphanidermatum. J Plant Physiol 163(12):1321-1332.<br /> <br /> Novy, R.G., A.M. Gillen and J.L. Whitworth. 2007. Characterization of the expression and inheritance of potato leafroll virus (PLRV) and potato virus Y (PVY) resistance in three generations of germplasm derived from Solanum etuberosum. Theor Appl Genetics 114:1161-1172.<br /> <br /> Novy, R.G., J.S. Miller, L.D. Porter, J. Whitworth, and H. Lozoya. 2007. Segregation of progeny of Solanum tuberosum subsp. andigena for foliar and tuber resistance to late blight. Am J Potato Res 84:106-107. (Abstract)<br /> <br /> Nunziata, A., V. Ruggieri, L. Frusciante, and A. Barone. 2007. Allele mining at the locus Gro 1 in Solanum wild species. Am J Potato Res 84:107. (Abstract)<br /> <br /> OBrien, M., M. Gray-Mitsumune, C. Kapfer, C. Bertrand, and D.P. Matton. 2007. The ScFRK2 MAP kinase kinase kinase from Solanum chacoense affects pollen development and viability. Planta 225:1221-1231.<br /> <br /> Osumi, T., W.R. Belknap, D.R. Rockhold, M.M. Maccree, K.F. McCue, and K.L. Deahl. 2007. United States Patent Number 7,148,397 Solanum bulbocastanum late blight resistance gene and use thereof December 2007. (Patent)<br /> <br /> Palumbo, R.E. and R.E. Veilleux. 2007. Variation of GFP expression in diploid and tetraploid transgenic potato. Am J Potato Res 84:393-401.<br /> <br /> Pelletier Y. and J. Dutheil. 2006. Behavioural responses of the Colorado potato beetle to trichomes and leaf surface chemicals of Solanum tarijense. Ent Exp Appl 120(2):125-130.<br /> <br /> Pelletier, Y., C. Clark and D. De Koeyer. 2007. Level and genetic variability of resistance to the Colorado potato beetle (Leptinotarsa decemlineata (Say)) in wild Solanum species. Am J Potato Res 84:143-148.<br /> <br /> Pino, M.-T., J.S. Skinner, E.-J. Park, Z. Jeknic, P.M. Hayes, M.F. Thomashow, and T.H.H. Chen. 2007. Use of a stress inducible promoter to drive ectopic AtCBF expression improves potato freezing tolerance while minimizing negative effects on tuber yield. Plant Biotech J 5:1-14, doi:10.1111/j.1467-7652.2007.00269.x.<br /> <br /> Qiu CaiLing, Li Yong, Bai YaMei, Lu WenHe. 2006. Distribution of protein content in diploid potato plants and their relations. Chinese Potato j 20(2):65-67.<br /> <br /> Radcliffe, E.B., D.W. Ragsdale and R.A. Suranyi. 2007. IPM case studies: seed potato. In: Aphids as crop pests (H.F. van Emden and R. Harrington, eds.) Wallingford, UK. CABI.<br /> <br /> Rorat, Tadeusz. 2007. Genes the expression of which is associated with acclimate to cold in Solanum sogarandinum. XV Ogolnopolskie Seminarium Sekcji Mrozoodpornosci, KNO PAN, Iinstytut Dendrologii PAN w Korniku, 24-25 maj 2007, -p. 3. (Abstract)<br /> <br /> Rorat, Tadeusz, Bartosz Szabala and Zhimin Yin. 2007. Expression of SK3-type dehydrin in transporting organsis associated with cold acclimation in Solanum species. 3rd Conference of Polish Society of Experimental Biology, Warsaw, 26-30 August 2007, p. 30.<br /> <br /> Roux, V. le, E.D.M. Campan, F. Dubois, C. Vincent, and P. Giordanengo. 2007. Screening for resistance against Myzus persicae and Macrosiphum euphorbiae among wild Solanum. Annals Appl Bio 151:83-88.<br /> <br /> Sattarzadeh, A., U. Achenbach, J. Lubeck, J. Strahwald, E. Tacke, H.R. Hofferbert, T. Rothsteyn, and C. Gebhardt. 2006. Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype PA2/3. Mol Breed 18(4):301-312.<br /> <br /> Scotti, N., L. Marechal-Drouard and T. Cardi. 2004. The rpl5-rps14 mitochondrial region: a hot spot for dna rearrangements in Solanum spp. somatic hybrids. Current Genet 45(6):378-382.<br /> <br /> ShuLi, Huang, Jin LiFeng, Du JiZhou, Li Hua, Zhao Qian, Ou GuangShuo, Ao GuangMing, and Yuan Ming. 2007. SB401, a pollen-specific protein from Solanum berthaultii, binds to and bundles microtubules and F-actin. Plant J 51(3):406-418.<br /> <br /> Simko, I., J.H. van den Berg, D. Vreugdenhil, and E.E. Ewing. 2007. Mapping loci for chlorosis associated with chlorophyll b deficiency in potato. Euphytica DOI 10.1007/s10681-007-9595-z.<br /> <br /> Sliwka, J., H. Jakuczun, R. Lebecka, W. Marczewski, C. Gebhardt, and E. Zimnoch-Guzowska. 2007. Tagging QTLs for late blight resistance and plant maturity from diploid wild relatives in a cultivated potato (Solanum tuberosum) background. Theor Appl Genet 115(1):101-112.<br /> <br /> Solomon-Blackburn, R.M., H.E. Stewart and J.E. Bradshaw. 2007. Distinguishing major-gene from field resistance to late blight (Phytophthora infestans) of potato (Solanum tuberosum) and selecting for high levels of field resistance. Theor Appl Genet 115(1):141-149.<br /> <br /> Stark, J.C., R.G. Novy, S.L. Love, J.L. Whitworth, D.L. Corsini, J.J. Pavek, A.R. Mosley, M.J. Pavek, N.R. Knowles, R.E. Thornton, S.R. James, D.C. Hane, N. Olsen, M.I. Vales, and C.R. Brown. 2007. Blazer Russet: An early to mid-season potato cultivar with high U.S. no. 1 yields and good processing and culinary qualities. Am J Potato Res 84:447-457.<br /> <br /> Stupar, R.M., P.B. Bhaskar, B.S. Yandell, W.A. Rensink, A.L. Hart, S. Ouyang, R.E. Veilleux, J.S. Busse, R.J. Erhardt, C.R. Buell, and J.M. Jiang. 2007. Phenotypic and transcriptomic changes associated with potato autopolyploidization. Genetics 176:2055-2067.<br /> <br /> Stushnoff, C., O. Kulen, P.E. Wilson, and D.G. Holm. 2007. Some antioxidant properties of purple potato cultivars and selections. Am J Potato Res 84:116-117. (Abstract)<br /> <br /> Suarez, S., E. Chaves and A.M. Clausen. 2007. Solanum tuber-bearing species behaviour against the false root-knot nematode Nacobbus aberrans. Am J Potato Res 84:117-118. (Abstract)<br /> <br /> Syverson, R., B.P. Millett and J.M. Bradeen. 2007. Analysis of haplotype frequency of the late blight resistance gene, RB, in Solanum bulbocastanum using a novel MAMA PCR-based approach. Plant & Animal Genome XV, San Diego, CA, Jan. 2007. (Abstract)<br /> <br /> Szabala, Bartosz, Zhimin Yin and Tadeusz Rorat. 2007. Expression of gene encoding a DHN24 dehydrin is associated with acclimation of Solanum sogarandinum to low temperature. XV Ogolnopolskie Seminarium Sekcji Mrozoodpornosci, KNO PAN, Iinstytut Dendrologii PAN w Korniku, 24-25 maj 2007, -str. 7. (Abstract)<br /> <br /> Tebbji, F., A. Nantel and D.P. Matton. 2007. Transcriptomic profiling of Solanum chacoense fertilization and embryogenesis. Plant Biology & Botany Joint Congress, American Society of Plant Biologists (ASPB), Chicago, Illinois, USA, 7-11 July, 2007. Poster presentation. (Abstract)<br /> <br /> Thompson, A., B. Farnsworth, N.C. Gudmestad, G. Secor, J. Sowokinos, D. Preston, M. Glynn, and H. Hatterman-Valenti. 2007. Dakota Diamond: A new, bright white skinned, chipping cultivar with exceptional yield and long-term storage potential. Am J Potato Res 84:118. (Abstract)<br /> <br /> Thompson, A.L., B.L. Farnsworth, G.A. Secor, N.C. Gudmestad, D. Preston, J.R. Sowokinos, M. Glynn, and H. Hatterman-Valenti. 2007. Dakota Crisp: A new high-yielding, cold-chipping potato cultivar with tablestock potential. Am J Potato Res 84:477-486.<br /> <br /> Thompson, A.L., R.J. Taylor, J.S. Pasche, R.G. Novy, and N.C. Gudmestad. 2007. Resistance to Phytophthora erythroseptica and Pythium ultimum in a potato clone derived from S. berthaultii and S. etuberosum. Am J Potato Res 84:149-160.<br /> <br /> Veilleux, R.E. and H. De Jong. 2007. Chapter 2: Potato. In: Genetic Resources, Chromosome Engineering, and Crop Improvement: Vegetable Crops. (Ram J. Singh, ed.) CRC Press LLC, Boca Raton, pp.17-58.<br /> <br /> Velishaeva, N.S., E.E. Khavkin and I.A. Shilov. 2006. Genotyping of potato and its wild relatives by the use of microsatellite analysis. Russian Ag Sci 9:1-3.<br /> <br /> Vyetrogon, K., F. Tebbji, D. Olsen, A.R.S. Ross, and D.P. Matton. 2007. A comparative proteome and phosphoproteome analysis of differentially regulated proteins during fertilization in the self-incompatible species Solanum chacoense Bitt. Proteomics 7:232-247. Cover picture of the issue.<br /> <br /> Wang, B. and A.R. Langille. 2007. Response of a gibberellin-deficient potato mutant to induction and growth regulators as a working model for tuber initiation. HortSci 42(3):540-543.<br /> <br /> Wang MiQia. 2007. Diversity and evolution of resistance genes in tuber-bearing Solanum species. Wageningen University, Wageningen Netherlands. 104 pp.<br /> <br /> Watkinson, J.I., L. Hendricks, A.A. Sioson, C. Vasquez-Robinet, V. Stromberg, L.S. Heath, M. Schuler, H.J. Bohnert, M. Bonierbale, and R. Grene. 2006. Accessions of Solanum tuberosum ssp. andigena show differences in phytosynthetic recovery after drought stress as reflected in gene expression profiles. Plant Sci 171(6):745-758.<br /> <br /> Whitworth, J., R. Novy, H. Lozoya, S. Yilma, and A. Mosley. 2007. Identification of potato breeding clones that confer high levels of late blight resistance to their progeny. Am J Potato Res 84:120-121. (Abstract)<br /> <br /> Whitworth, J.L., R.G. Novy, H. Lozoya-Saldana, S. Yilma, A.R. Mosley, and M.I. Vales. 2007. Multiple-site identification of potato parent clones conferring high levels of late blight resistance with a corresponding genetic model for resistance. Am J Potato Res 84:313-321.<br /> <br /> Winton, L.M., R.H. Leiner, A.L. Krohn, and K.L. Deahl. 2007. Occurrence of Late Blight caused by Phytophthora infestans on potato and tomato in Alaska. Plant Disease 91:634.<br /> <br /> Young, Gillian K., Louise R. Cooke, William W. Kirk, Pavani Tumbalam, Frances M. Perez, and Kenneth L. Deahl. 2007. The influence of competition and host plant resistance on selection of Phytophthora infestans populations in Michigan State and Northern Ireland. PhD Thesis 12/07. Paper submitted to European Journal of Plant Disease. <br /> <br /> Zhang, L.-H., H. Mojtahedi, H. Kuang, B. Baker, and C.R. Brown. 2007. Marker-assisted selection of Columbia root-knot nematode resistance introgressed from Solanum bulbocastanum. Crop Sci 47:2021-2026.<br /> <br /> Zhang, L.-H., H. Mojtahedi, H. Kuang, B. Baker, and C.R. Brown. 2007. Fine mapping and isolation of RMc1(blb) locus to Columbia root-knot nematode resistance from Solanum bulbocastanum. Am J Potato Res 84:123. (Abstract) <br /> <br /> C. Theses Reporting Research with NRSP-6 Stocks<br /> <br /> Chantha, Sier-Ching. 2007. Caractérisation fonctionnelle des gènes NOTCHLES et MIDASIN lors du développement végétal. January 2007. PhD Thesis, University of Montreal, Canada.<br /> <br /> Cortes Albornoz, Carolina. 2007. Determinacion del grado de hibridacion entre papa (Solanum tuberosum) y especies silvestres relacionadas a partir de polinizacion dirigida (Determination of the hybridization level among potato (Solanum tuberosum) and related wild species by manual pollinization). Thesis to obtain the title of Agricultural Engineer. University of Chile, Faculty of Agronomy.<br /> <br /> Germain, Hugo. 2007. Caractérisation chez Solanum chacoense et Arabidopsis thaliana dun homologue du gène Notchless de la Drosophile impliqué dans la différentiation et le destin cellulaire. August 2007. PhD Thesis, University of Montreal, Canada.<br /> <br /> Syversoon, R.L. 2007. Towards development of molecular resources in disease resistant wild potato Solanum bulbocastanum, including a linkage map and SNP-based markers. MS Thesis, University of Minnesota.<br />

Impact Statements

1. Potato is the number one vegetable crop and ranks number four among world food crops. The US Potato Genebank provides a high quality, ready source of seed, technology and information to support potato enhancement, breeding and research in the USA and around the world.
2. The impact of the genebank has been strong in the past. Staff have been instrumental in developing technologies widely used in potato breeding, like cut-stem pollination, hormone pre-treatment of seeds for better germination, extraction of haploids and use with 2n gametes in breeding. The genebank imported valuable germplasm and generated, formatted and computerized a wide array of trait evaluation data and taxonomic characterization. One way the impact of these contributions can be measured is by the occurrence of NRSP-6 germplasm in the pedigrees of new, improved potato cultivars. About 70% of all potatoes grown in the United States have germplasm in their pedigrees from the genebank. Three cultivar releases were published in the American Journal of Potato Research in 2007: Blazer Russet, Dakota Crisp and MegaChip. Each is known to contain wild species.
3. The impact of genebank contributions is also evident in the numerous publications in 2007 that provide information that ultimately contribute to better exploitation of the germplasm resource. Publications report section lists 89 papers, 53 abstracts, 1 patent, 2 patents pending and 4 theses which report the use of NRSP-6 Solanum introductions this year.
4. The impact of the genebank is expected to increase in the future for several reasons. 1) Mutants discovered and characterized by staff will be increasingly valuable as research models. 2) Intragenic transformation of potato has now been demonstrated and identified as a kind of GMO much more accepted by the consumer, so useful exotic potato genes will be increasingly valuable as the technology to easily insert them into existing cultivars improves. 3) Potato is rapidly expanding in large new growing regions, so the need for genetic resources for breeding in new environments and for new tastes will surge. 4) The collection and organization of data at the genebank for an ever-increasing array of traits (e.g., nutritional) is expected to become more and more critical for breeding efficiency.
5. The genebank&lsquo;s stocks are a critical resource to address the pressure on the industry from extreme increases in costs of inputs: e.g., fertilizers, fungicides, irrigation. NRSP6 cooperators are using the genebank&lsquo;s diversity to make an impact through new cultivars that have, for example, resistance to later blight, early blight, pink rot, powdery scab; and can produce high yields of quality potatoes with about 2/3 of normal fertilizer inputs.

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Report Information

Participants

Ashworth, Edward;
Guthrie, Richard;
Hu, CY;
Jahn, Molly;
Miller, J. Creighton;
Brown, Charles;
Douches, David;
Bizimungu, Benoit;
Thro, Ann Marie;
Danforth, Harry;
Chandler, Larry;
Simon, Phil;
Abad, Jorge;
Hoopes, Robert;
Palta, Jiwan;
Navarre, Felix;
Thill, Christian;
Brancel, Ben;
Genebank staff: Bamberg / Martin / Spooner / del Rio

Brief Summary of Minutes

All informational files plus the minutes of this meeting are contained in the atttachment link at the end of this section with title "Copy of Minutes":

- ANNUAL REPORT CY08

- GENEBANK UPDATES SINCE 2008 ANNUAL REPORT

- TAC09 MEETING AGENDA

- TAC09 MINUTES

- TAC09 RESOLUTIONS

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NRSP-6 TAC09 ANNUAL MEETING

Peninsula Ag Research Station / Sturgeon Bay, Wisconsin

July 8

Chair David Douches called the meeting to order at 8:30am.

Walter De Jong (secretary) was not able to attend this meeting. Jiwan Palta volunteered to take the minutes of the meeting.

Ben Brancel is representing Dean Molly Jahn, who would come to meeting around noon. She would like to have few minutes to talk to the group.

Benoit Bizimungu is representing Richard Tarn who retired from his position recently.

Chair asked for the approval of the agenda for 2009 meeting. C. Miller moved, J. Palta second. Motion approved unanimously.

John Bamberg reported that genebank has accomplished the tasks they were directed to do at the 2008 TAC meeting. Impact statements were added to the Annual Report. Progress was made in seed increase techniques. Enhanced communications to clientele (farmers, citizens of the state) on the importance of the genebank. John and Max attended each of the four regional SAESD spring meetings and gave presentations on NRPS6 to the directors.

Chuck Brown indicated that a pie chart outlining the state and federal support would be useful.

Ed Ashworth asked the group about the possibility of generating support for the genebank from private foundations. J. Palta indicated that in the past Rockefeller Foundation supported potato research. There is a growing public interest in "green/sustainable" agriculture, with which NRSP6 is a good fit.

Chuck Brown indicated that there is a perception that we are releasing too many varieties as compared to what the growers can handle. Discussion followed on developing varieties in relation to sustainability e.g. better water use efficiency, better soil nutrient utilization efficiency, better human nutrition, etc. This could give industry such as McDonalds to advertize that potatoes they serve are produced with less chemical and water input etc (sustainability rating).

Robert Hoopes indicated that although several traits such as high yield, dry matter, storability, etc., are of interest to the industry, the concept of locally grown is important to the public. In addition, lowering acrylamide is one of the top priorities.

Chuck Brown informed that there will be a one day brainstorming session at the Potato Expo in January 2010. There will be attempt to produce a PAA-NPC document on research priorities at the national level. J. Palta suggested that genebank could have a booth at the Potato Expo. Everyone was positive about this idea. C. Brown will call John Keeling to see if the booth fee could be waived.

Chair appointed resolution committee: Chuck Brown, Creighton Miller

Last year's minutes approved: Brown moved and Thill seconded; approved unanimously.

Agenda Item 5. Lead AA

C.Y. Hu indicated that AA should come from NC region. Lead AA is decided by the four regional AAs. After some discussion it was suggested that Molly Jahn be approached for the lead AA. Ben Brancel indicated that Molly Jahn is interested in finding long term sustainability for the genebank. She is supportive of finding new sources of funding. C.Y. Hu noted that renewal document should be written this fall. This is to include review of the progress over past 5 years and proposed project for next 5 years. Current project will end Sept. 2010. All NRSPs are going through review process. NRSP-6 received support from the Western and NC directors and not much from the NE directors. Richard Guthrie can solidify support from the southern directors. The 1% OTT funding which is currently the source of the NRSP6 budget is not mandated by law, and could be rescinded by the SAES directors. Larry Curtis has been appointed as the new AA for the WR.

C. Miller thanked C. Y. for the outstanding job he has done as the lead AA.


Agenda Item 6: Other regional AAs

Ed Ashworth - NE: This project is sitting in a vulnerable position. There is some good news. Senate yesterday approved 4% increase in Hatch dollars. Rikki Sterret's position has been filled. Large amount of potatoes are shipped from West and Midwest to East (high consumption area). Greg Porter is looking into late blight resistant cultivars to reduce the weekly applications of fungicides currently used in the East.

Richard Guthrie - S: John Bamberg's visit to the spring directors' meeting made a good impression, particularly in showing the substantial support the genebank is already receiving from sources other than MRF. Richard would retire before the meeting next year. It was suggested that to generate support of NRSP-6, a one page agenda brief on NRSP-6 should be submitted to the regional meetings each year that an in-person report is not presented.

Molly Jahn - NC: After lunch Dean Molly Jahn made some comments regarding support of the Potato genebank. She indicated that with the consistent reduction in state funding we have to look for alternative ways (Business Model) to support the genebank. The UW-CALS investment in potato research and extension is very high and is second to dairy. Ben Brancel will be helping in this. Molly indicated that she would identify a senior person who could serve in the Lead AA role.

Agenda Item 7. Regional and ARS Tech Representatives

Chuck Brown - ARS: Nine scientists provided reports. Kathy Haynes is expanding germplasm base resistant to late blight. Rich Novy is looking at Potato Cyst Nematode resistance and Dennis Halterman looking for genes similar to RB. Chuck Brown evaluated 50 accessions from the Andigena core collection and seems to have found good resistance to black dot. Reported on an excellent example of a success story of taking the NRSP6 germplasm and transferring disease resistance to develop materials. Took 20 years to produce an advanced clone with multiple resistances to root rot nematode using S. bulbocastanum fusion hybrids of John Helgeson. Resistance against cold sweetening in Premier Russet came from Solanum phureja. Ranger Russet is being replaced by Alturas and Umatilla.

Walter DeJong - NE: Could not attend the meeting but provided this information: A total of 359 units of germplasm were distributed in the Northeast region, for purposes ranging from testing old cultivars for possible adoption by organic growers, to studying the genetics of tuber flesh pigmentation, to taking photographs for books and magazines.

Creighton Miller - S: Summarized activities. Major emphases is Zebra Chip. There have been multimillion-dollars losses to the chip industry. Two years ago Texas gave $800,000 for research effort in this area for a two-year period. Primary support was for pathologist. Some support came to the breeding program. Eight selections have been identified that are free of Zebra chip when planted in the presence of natural inoculums. Another project is looking for resistance at the species level. Eventually we may have to look at wild species for the sources of resistances. Robert Hoopes indicated that this is a serious problem for the chip industry. Craig Yencho is looking at S. phureja as a source for heat resistance. Richard Veilleux's doubled monoploids of S. phureja from the genebank are the basis of the potato genome sequencing project. The Miller lab has found strong anti-cancer properties in extracts of Solanum jamesii from the genebank.

Isabel Vales - W: Isabel Vales could not attend the meeting but sent a report including this information: During 2008, the Potato Introduction Station (NRSP-6, Sturgeon Bay, WI) supplied potato materials to the following Western states: Alaska, California, Idaho, Oregon, and Washington. Many potato clones used in Western breeding were derived from material provided by NRSP6: PVY resistance from andigena, S. stoloniferum; Potato Tuber Worm (PTW) resistance from S. berthaultii; late blight resistance from S. acaule, S. demissum, S. phureja, S. bulbocastanum, and S. tuberosum spp. andigena.

Dave Douches - NC: Dave Douches: Evaluating a diploid population for Colorado Potato Beetle resistance. Also evaluating S. microdontum selections for tuber and foliar late blight resistance in cooperation with genebank staff, and they have identified a potent R gene. Seven species are being used in the breeding program for variety development. Germplasm is being evaluated for ornamental potential. Mentioned use of germplasm for looking for natural genetic variation for PVY resistance and the great potential for intragenic transformation developed by Simplot for using potato genes mined from the NRSP6 genebank stocks. Reviewed the upcoming SOLCAP workshop scheduled for Sunday August 9th at the PAA meeting at Fredericton, New Brunswick. Douches lab found that there was a correlation between acrylamide and chip color and sugar over a broad range of tuber sugar contents. However, in the very low sugar range (0.001 to 0.002) there was no correlation. Moisture levels and fructose contents of the tuber can affect acrylamide.

Other reports

Christian Thill: Major effort is being directed to select for late blight, PVY and common scab. Have developed a promising technology using liquid nitrogen freezing of the meristem cells to eliminate virus contamination.

Jiwan Palta: In addition to yield, grade, disease resistance and pest resistance, the breeding program has a major effort on tuber internal quality, storage and fry quality, scab resistance and late blight resistance. Several species and progenies thereof are being screened for health related traits such as K+. Numbers of species are also being looked at for desirable traits such as tuber Ca, cold tolerance and soft rot resistance. The Wisconsin program is a closely integrated with the genebank's evaluation mission.

Agenda Item 8 Agriculture and Agrifood Canada.

Benoit Bizimungu : Richard Tarn retired. Benoit Bizimungu is moving and consolidating the potato breeding program in Fredericton. His program has utilized S. oplocense, S. chacoense and S. pinnatissectum. The breeding program emphases include potato beetle resistance, bacterial wilt resistance, drought resistance and new byproducts.

Agenda Item 9: Industry Perspective

Robert Hoopes: Frito Lay gets potatoes from every region. Frito Lay has had long-term relationship with the genebank. Have used varieties over the years that have 15-20 species in their pedigrees. All varieties have S. chacoense in their pedigrees. PVY resistance obtained from S. stoloniferum. S. berthaultii may have Zebra chip resistance. Dan Ronis' program is cooperating with genebank staff on nutritional and quality properties like starch ratio, acrylamide (low asparagine, low pH) and high tuber calcium accessions. Some studies have shown that blanching can reduce acrylamide by 90% but the texture of the chip was influenced.

Simplot representative Caius Rommens' travel plans were thwarted at the last minute, but has sent a report / letter of support that will be included in the project renewal document. Simplot is setting up a new research farm near Boise, ID.

Agenda Item 11: Collecting and Taxonomy

David Spooner distributed several copies of his publications. He pointed out that Jack Hawkes had documented 232 potato species. His research has shown that many of these should be combined, and now the number of species is less than half.

Agenda Item 10: NRSP-6 report and FY 10 outlook

See "ar2008.doc" at http://www.ars-grin.gov/nr6/admin.html for Annual Report, and interim update report "Genebank News and Activities since 2008 Annual Report.doc". ALL OF THESE FILES PLUS THE RECORD OF THIS MEETING ARE CONTAINED IN THE NIMSS ATTACHMENT TO THIS SECTION "Copy of Minutes" IN PROPER FORMAT FOR VIEWING AND PRINTING)

John Bamberg asked the group to provide feedback on the NRSP-6 report and impact statements which are posted on the website. Alfonso del Rio is continuing to perform technical research to help the genebank find the most efficient methods to preserve the maximum genetic diversity. Recent developments in evaluation include an emphasis on consumer-oriented traits, particularly nutritional ones like antioxidants, potassium, glycemic index, pH, satiety value, and anticancer agents. The genebank is also pursuing multiplex comprehensive evaluation of S. microdontum, which has numerous valuable traits and is quite easily introgressed into the tuberosum breeding pool.

Budget: University of Wisconsin salary support to the genebank discontinued at the end of FY08. Molly Jahn pointed out that state funded research support is threatened at this time. All state employees are subjected to furlough for 8 days. She indicated that we have to look for alternate sources of support possibly approaching foundations (including Mc Donald's etc.) and others. Ben Brancel suggested that we should create a list that captures must do, should do, and would do (with minimum support). We should look for opportunities for partnerships. John Bamberg pointed out that genebank staff are already vigorously pursuing such extramural funds and opportunities to achieve greater efficiency, and that the program has already significantly down-sized. He asked the group if a budget of $150,000 should be proposed. C. Y. Hu indicated that they will not vote for no more than $150,000. Ed Ashworth suggested that a request should be made for $150,000 and an additional amount proportional to the increase associated with Hatch increase.

Project-Renewal Writing: The following were specified to be on the renewal writing team: Ann Marie Thro, Chuck Brown, CY Hu, Molly Jahn, Larry Chandler, Peter Bretting and Christian Thill. John Bamberg will prepare a draft soon.

Agenda Item 13: CSREES (Ann Marie Thro)

Ann Marie Thro provided an update on the budget and CSREES in general. Potato special grant budget is 9% less this year. Increases in Hatch and AFRI budgets for 2010 are proposed by the President. Dr. Rajiv Shah is going to be the new Undersecretary of Agriculture. CSREES will have a name change to National Institute for Food and Agriculture. NRI is replaced and expanded by AFRI. It will include plant and animal breeding research (see synopsis at: www.csrees.usda.gov/funding/afri/afri_synopsis.html). Ann Marie pointed out that non-technical summary in CRIS reports is very important to communicate impact. Also very important to acknowledge grants and agency in all publications.

Agenda Item 14: APHIS/ Quarantine (Jorge Abad)

Jorge Abad explained the procedure for bringing potato germplasm into the country. They (Joe Foster, leader) screen for all virus and bacterial diseases. Zebra chip has appeared in tomato. Last year two new viruses were intercepted (JCM-23, JCM-79).

Meeting adjourned at 5:30pm followed by supper at "The Log Den".

July 9

Chairman Dave Douches called the meeting to order at 8:30am.

Dave Douches reported that there is an exhibit at the National Botanical Gardens on the Mall by the Capitol in Washington DC. John Bamberg has been asked to present there in the fall of 2010. With assistance from USPB (John Keeling) an exhibit featuring potato transgenic work from MSU is on display there, including a video of transgenic potato displaying resistance to Colorado potato beetle (shown to the group).

Agenda item 15: Review and approve resolutions

Four resolutions were unanimously approved (see http://www.ars-grin.gov/nr6/admin.html or "Copy of Minutes" link at the end of this section for full text):


1: Memorial for Robert Hougas, first genebank Project Leader.

2. Appreciation to CY Hu for outstanding leadership as Lead AA.

3. Appreciation to Project staff for hosting the current meeting.

4. Appreciation to Richard Guthrie for excellent service as SR AA

Agenda item 16: Elect new officers and set next meeting

Moved and unanimously approved that the current officers advance for the upcoming year: Isabel Vales = Chair, Walter DeJong = Vice Chair, and that Creighton Miller serve as secretary.

The 2010 meeting will be joint with the July 26-29 RTAC/PGOC/CGC Chairs' meeting at Geneva, NY.

Creighton Miller moved to adjourn the business meeting, second by J. Palta and unanimously accepted.

Tour of genebank followed until about noon.

Respectfully Submitted,
Jiwan Palta

Accomplishments

A complete report of Accomplishments, Impact Statements, and Publications, properly formatted for viewing and printing is provided at http://www.ars-grin.gov/nr6/admin.html "ar2008.doc" and attached under the Publications section following.

Publications

A complete report of Publications, Accomplishments, and Impact Statements, properly formatted for viewing and printing is provided at http://www.ars-grin.gov/nr6/admin.html and under the link "Copy of Minutes" in meeting minutes section above<br /> <br /> ==============================================<br /> <br /> Publications issued by NRSP-6 Personnel<br /> <br /> ---------------------------------------<br /> <br /> Alvarez, N.M.B., I.E. Peralta, A. Salas, and D.M. Spooner. 2008. A morphological study of species boundaries of the wild potato Solanum brevicaule complex: replicated field trials in Peru. Pl Syst Evol 274:37-45.<br /> <br /> Ames, M. and D.M. Spooner. 2008. DNA from herbarium specimens settles a controversy about origins of the European potato. Am J Bot 95(2):252-257. (Additional supplemental data)<br /> <br /> Ames, M., A. Salas and D.M. Spooner. 2008. A morphometric study of species boundaries of the wild potato Solanum series Piurana (Solanaceae) and putatively related species from seven other series in Solanum sect. Petota. Syst Bot 33:566-578.<br /> <br /> Bamberg, J.B. 2008. Genetic comparisons of gibberellin mutants in potato. Am J Potato Res 85:2. (Abstract)<br /> <br /> Bamberg, John and Alfonso H. del Rio. 2008. Proximity and introgression of other potato species does not explain genetic dissimilarity between Solanum verrucosum populations of Northern and Southern Mexico. Am J Potato Res 85:232-238.<br /> <br /> Bamberg, J.B., C.J. Fernandez, M.W. Martin, and J.J. Pavek. 2008. Tuber dormancy lasting eight years in the wild potato Solanum jamesii. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Bamberg, J.B., M.W. Martin and J.P. Palta. 2008. Variation in Solanum species tuber potassium accumulation and its implication for human nutrition. Am J Potato Res 85:2. (Abstract)<br /> <br /> Belmar-Diaz, C., H. Lozoya-Saldana, M. Salgado, and J. Bamberg. 2008. Phytophthora infestans: races and genotypes in Toluca, Mexico. A two-year update. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Del Rio, A.H. and J.B. Bamberg. 2008. Unbalanced bulk of parents seed is not detrimental in potato germplasm regeneration. Am J Potato Res 85:28. (Abstract) <br /> <br /> Del Rio, A.H., J.B. Bamberg, C. Fernandez, and C. Zorrilla. 2008. Update on the comparative assessment of genetic diversity between accessible and remote potato populations: AFLP analysis of wild potato Solanum stoloniferum (formerly S. fendleri) distributed in SW regions of the USA. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Fajardo, D., R. Castillo, A Salas, and D.M. Spooner. 2008. A morphometric study of species boundaries of the wild potato Solanum series Conicibaccata: a replicated field trial in Andean Peru. Syst Bot 33:183-192. <br /> <br /> Hale, Anna L., Lavanya Reddivari, M. Ndambe Nzaramba, John B. Bamberg, and J. Creighton Miller, Jr. 2008. Interspecific variability for antioxidant activity and phenolic content among Solanum species. Am J Potato Res 85:332-341.<br /> <br /> Jansky, S.H., R. Simon and D.M. Spooner. 2008. A test of taxonomic predictivity: resistance to early blight in wild relatives of cultivated potato. Phytopath 98:680-687.<br /> <br /> Jimenez, J.P., A. Brenes, A. Salas, D. Fajardo, and D.M. Spooner. 2008. The use and limits of AFLP data in the taxonomy of polyploid wild potato species in Solanum series Conicibaccata. Conserv Genet 9:381-387.<br /> <br /> Nzaramba, M.N., L. Reddivari, J.B. Bamberg, and J.C. Miller Jr. 2008. Phenolic and glycoalkaloid levels of S. jamesii accessions and their anti-proliferative effect on human prostate and colon cancer cells in vitro. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Palta, J.P., J.B. Bamberg and S.E. Vega. 2008. Moving frost hardy genes from wild to cultivated potatoes. Use of precise screening tools to make real progress. Am J Potato Res 85:23. (Abstract)<br /> <br /> Palta, J.P., J.B. Bamberg and S.E. Vega. 2008. Freezing tolerance of cultivated potatoes: Moving frost hardy genes from wild potatoes and making real progress using precise screening tools. ASHS 2008 Conference, Orlando, Florida. HortSci 43:1108. (Abstract)<br /> <br /> Palta, J.P., J.B. Bamberg, S.E. Vega, F.M. Navarro, and B. Bowen. 2008. Genetic improvement of potato for tuber calcium uptake. Proceedings of Annual Wisconsin Potato Meetings 21:15-20.<br /> <br /> Palta, J.P., R. Gomez, A.H. del Rio, W. Roca, J.B. Bamberg, A. Salas, and M. Bonierbale. 2008. Supplemental calcium nutrition may have the potential of improving tuber yield of native potatoes in the Peruvian highlands. Am J Potato Res 85:23. (Abstract)<br /> <br /> Palta, J.P., F.M. Navarro, J.B. Bamberg, S.E. Vega, and B. Bowen. 2008. The Calcium Solution: Developing potato cultivars with enhanced tuber storage and internal quality by genetic improvement of tuber calcium accumulation ability. The Badger Common Tater 60(11):14-16.<br /> <br /> Pendinen G., T. Gavrilenko, J. Jiang, and D.M. Spooner. 2008. Allopolyploid speciation of the Mexican tetraploid potato species Solanum stoloniferum and S. hjertingii revealed by genomic in situ hybridization. Genome 51:714-720.<br /> <br /> Peralta, I.E., D.M. Spooner and S. Knapp. 2008. The taxonomy of tomatoes: a revision of wild tomatoes (Solanum section Lycopersicon) and their outgroup relatives in sections Juglandifolium and Lycopersicoides. Syst Bot Monogr 84:1-186+3 plates.<br /> <br /> Spooner, D.M., D. Fajardo and A. Salas. 2008. Revision of the Solanum medians complex (Solanum sect. Petota). Syst Bot:33:579-588.<br /> <br /> Spooner, D.M., F. Rodriquez, Z. Polgar, H.E. Ballard Jr., and S.H. Jansky. 2008. Genomic origins of potato polyploids: GBSSI gene sequencing data. The Pl Genome, a Suppl. to Crop Sci. 48(S1):S27-S36. <br /> <br /> Journal Articles and Abstracts Reporting Research with NRSP-6 Stocks<br /> <br /> --------------------------------------------------------------------<br /> <br /> <br /> Almasia, N.I., A.A. Bazzini, H.E. Hopp, and C. Vazquez-Rovere. 2008. Over expression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. Mol Pl Path 9(3):329-338. (Abstract)<br /> <br /> Arvin, M.J. and D.J. Donnelly. 2008. Screening potato cultivars and wild species to abiotic stresses using an electrolyte leakage bioassay. J Ag Sci Tech 10(1):33-42. (Abstract)<br /> <br /> Aversano, R., M.R. Ercolano, L. Frusciante, L. Monti, J.M. Bradeen, G. Cristinzio, A. Zoina, N. Greco, S. Vitale, and D. Carputo. 2007. Resistance traits and AFLP characterization of diploid primitive tuber-bearing potatoes. Genet Res Cr Evol 54(8):1797-1806. (Abstract)<br /> <br /> Ballou, S.M., K.Y. Yun, C. Cheng, and B.G. de los Reyes. 2007. Cold sensitivity gradient in tuber-bearing Solanum based on physiological and transcript profiles. Crop Sci 47(5):2027-2035. (Abstract)<br /> <br /> Bhaskar, P.B., J.A. Raasch, L.C. Kramer, P. Neumann, S.M. Wielgus, S. Austin-Phillips, and J.M. Jiang. 2008. Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight. BMC Pl Bio 8(8). (Abstract)<br /> <br /> Bidani, A., O. Nouri-Ellouz, L. Lakhoua, D. Sihachakr, C. Cheniclet, A. Mahjoub, N. Drira, and R. Gargouri-Bouzid. 2007. Interspecific potato somatic hybrids between Solanum berthaultii and Solanum tuberosum L. showed recombinant plastome and improved tolerance to salinity. Pl Cell, Tissue & Organ Culture 91(3):179-189. (Abstract)<br /> <br /> Bradeen, J.M., M. Iorizzo, H. Mann, L. Gao, E.A. Quirin, R. Aversano, and D. Carputo. 2008. Comparative structural genomics of 1EBN potato: DArT markers for improved access to resistance genes. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Brown, C.R. 2008. Breeding for phytonutrient enhancement of potato. Am J Potato Res 85:298-307.<br /> <br /> Brown, C.R., D. Culley, M. Bonierbale, and W. Amoros. 2007. Anthocyanin, Carotenoid content, and antioxidant values in native South American potato cultivars. HortSci 42(7):1733-1736. (Abstract)<br /> <br /> Brown, C.R., M. Moore, M. Pavek, D. Hane, R. Novy, J.C. Miller Jr., S.L. Love, and S. James. 2008. Genetic variability in mineral content of potato tubers. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Burkhart, C.R., B.J. Christ, K.G. Haynes, and B.T. Vinyard. 2008. Little genetic and no additive genetic variance for resistance to common scab in a Solanum phureja-S. stenotomum population. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Poster)<br /> <br /> Camadro, E.L., S.K. Saffarano, J.C. Espinillo, M. Castro, and P.W. Simon. 2008. Cytological mechanisms of 2n pollen formation in the wild potato Solanum okadae and pollen-pistil relations with the cultivated potato, Solanum tuberosum. Genet Res Crop Evol 55(3):471-477. (Abstract)<br /> <br /> Campbell, B.A., J. Hallengren and D.J. Hannapel. 2008. Accumulation of BEL1-like transcripts in solanaceous species. Planta 228:897-906.<br /> <br /> Cernak, I., J. Taller, I. Wolf, E. Feher, G. Babinszky, Z. Alfoldi, G. Csanadi, and Z. Polgar. 2008. Analysis of the applicability of molecular markers linked to the PVY extreme resistance gene Rysto, and the identification of new markers. Acta Bio Hungarica 59(2):195-203. (Abstract)<br /> <br /> Chatterjee, M., A.K. Banerjee and D.J. Hannapel. 2007. A BELL1-like gene of potato is light activated and would inducible. Pl Physio 145(4):1435-1443. (Abstract)<br /> <br /> Cheng, ShanHan, Xie CongHua, Lin ShiSen et al. 2006. Germplasm enhancement and breeding to resist low temperature sweetening in potato. China Vegetables pp 84-88. (Abstract)<br /> <br /> Coleman, W.K. 2008. Evaluation of wild Solanum species for drought resistance: 1. Solanum gandarillasii Cardenas. Environ Exp Bot 62(3):221-230. (Abstract)<br /> <br /> Dhoop, B.B. 2008. Association mapping in tetraploid potato using SSRs and AFLPS. In: 17th Triennial Conference of the EAPR, Abstracts of papers and posters, Brasov, Romania, July 6-10, 2008, p. 376. (Abstract)<br /> <br /> Dhoop, B.B., M.J. Paulo, K. Kowitwanich, R.G.F. Visser, H.J. van Eck, and F.A. van Eeuwijk. 2008. Association mapping in tetraploid potato using SSRs and AFLPs. In: Molecular mapping and marker assisted selection in plants, Programme and abstracts, Vienna, Austria, February 03-06, 2008, p. 88. (Abstract)<br /> <br /> Douches, D., J. Coombs, J. Estelle, D. Berry, K. Zarka, W. Kirk, and R. Schafer. 2008. Update on late blight resistance breeding in Michigan. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Drobyazina, P. and E. Khavkin. 2008. Two CONSTANS-LIKE1 genes in long- and short-day Solanum plants. XVI FESPB Congress, Tampere, Finland, 2008. Physiol Pl 133:10-20. (Abstract)<br /> <br /> Drobyazina, P.E. and E.E. Khavkin. 2008. Two CONSTANS-LIKE genes in Solanum plants. Control of flowering time and application for plant breeding. Science meeting, Salzau, German, September 2008, p 35. (Abstract)<br /> <br /> Fry, W. 2008. Phytophthora infestans: the plant (and R gene) destroyer. Mol Pl Path 9(3):385-402. (Abstract)<br /> <br /> Ginzberg, I., J. Tokuhisa and R.E. Veilleux. 2008. Potato steroidal glycoalkaloids: Biosynthesis and genetic manipulation. Potato Res 52:0014-3065 (Print) 1871-4528 (Online).<br /> <br /> Gokce, A., M.E. Whalon, H. Cam, Y. Yanar, I. Demirtas, and N. Goren. 2007. Contact and residual toxicities of 30 plant extracts to Colorado potato beetle larvae. Archives Phyto Pl Prot 40(6):441-450. (Abstract)<br /> <br /> Gonzalez-Schain, N.D. and P. Suarez-Lopez. 2008. CONSTANS delays flowering and affects tuber yield in potato. Bio Plant 52(2):251-258. (Abstract)<br /> <br /> Govers, F. and H.J.G. Meijer. 2007. Phytophthora genomics: new opportunities and challenges. Gewasbescherming 38(5):265-271. (Abstract)<br /> <br /> Greco, N., A. Brandonisio, and P. de Cosmis. 2007. Pathotypes and heterogeneity of Italian populations of Globodera rostochiensis and G. pallida. Nematol Mediterranea 35(2):137-142. (Abstract) <br /> <br /> Halterman, D., Z. Liu, Y. Chen, and S. Stephenson. 2008. Molecular characterization of potato disease resistance genes. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Halterman, D.A., L.C. Kramer, S. Wielgus, and J.M. Jiang. 2008. Performance of transgenic potato containing the late blight resistance gene RB. Pl Disease 92(3):339-343. (Abstract)<br /> <br /> Hannapel, D.J., M. Chatterjee, Y.Y. Yu, H. Chen, and A.K. Banerjee. 2008. A light-activated signal for tuberization. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Haynes, Kathleen G. 2008. Heritability of chip color and specific gravity in a long-day adapted Solanum phureja-S. stenotomum population. Am J Potato Res 85:361-366.<br /> <br /> Haynes, K.G., B.J. Christ and B.T. Vinyard. 2008. Determining the importance of combining ability for late blight resistance in early generations of potato breeding when susceptible clones are discarded. Am J Potato Res 85:445-454.<br /> <br /> Heldak, J., M. Bezo, V. Stefunova, and A. Gallikova. 2007. Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F1 progenies. Czech J Genet Pl Breed 43(4):125-134. (Abstract)<br /> <br /> Hermanova, V., J. Barta and V. Curn. 2007. Wild potato species: Characterization and biological potential for potato breeding. Czech J Genet Pl Br 43(3):73-81. (Abstract)<br /> <br /> Horgan, F.G., D.T. Quiring, A. Lagnaoui, and Y. Pelletier. 2007. Variable responses of tuber moth to the leaf trichomes of wild potatoes. Ent Exp Appl 125(1):1-12. (Abstract)<br /> <br /> Horgan, F.G., D.T. Quiring, A. Lagnaoui, A.R. Salas, and Y. Pelletier. 2007. Periderm- and cortex-based resistance to tuber-feeding Phthorimaea operculella in two wild potato species. Ent Exp Appl 125(3):249-258. (Abstract)<br /> <br /> Houser, A.J. and R. Davidson. 2008. Development of a greenhouse assay to evaluate potato germplasm for susceptibility to powdery scab. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Jacobs, M.M.J., R.G. van den Berg, V.G.A.A. Vleeshouwers, M. Visser, R. Mank, M. Sengers, R. Hoekstra, and B. Vosman. 2008. AFLP analysis reveals a lack of phylogenetic structure with Solanum section Petota. BMC Evol Bio 88(145). (Abstract)<br /> <br /> Jansky, S.H., A.J. Hamernik and J. Bae. 2008. The use of Solanum verrucosum to access 2x, 1EBN germplasm. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Jolivet, K., E. Grenier, J.P. Bouchet, M. Esquibet, M.C. Kerlan, B. Caromel, D. Mugniery, and V. Lefebvre. 2007. Identification of plant genes regulated in resistant potato Solanum sparsipilum during the early stages of infection by Globodera pallida. Genome 50(4):422-427. (Abstract)<br /> <br /> Kielbowicz-Matuk, Agnieszka, Pascal Rey and Tadeusz Rorat. 2008. The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J Exp Bot, 4/25/2008, pp 1-13.<br /> <br /> Lang, ZhiHong, Zhou Peng, Yu JingJuan, Ao GuangMing, and Zhao Qian. 2008. Functional characterization of the pollen-specific SBgLR promoter from potato (Solanum tuberosum L.). Planta 227(2):387-396. (Abstract)<br /> <br /> Le Roux, V., E.Campan, F. Dubois, C. Vincent, and P Giordanengo. 2007. Screening for resistance against Myzus persicae and Macrosiphum euphorbiae among wild Solanum. Ann. Appl. Biol. 151:83-88.<br /> <br /> Le Roux, V., S. Dugravot, E.Campan, F. Dubois, C. Vincent, and P Giordanengo. 2008. Wild Solanum Resistance to Aphids: Antixenosis or Antibiosis? J. of Econ. Entomol. 101 (2): 584-591<br /> <br /> Liu, XiCai. 2006. Study and utilization of potato germplasm resources. China Vegetables pp 25-27. (Abstract) <br /> <br /> Love, S.L. and J.J. Pavek. 2008. Positioning the potato as a primary food source of vitamin C. Am J Potato Res 85:277-285.<br /> <br /> Luthra, S.K., J. Gopal, V. Kumar, B.P. Singh, and S.K. Pandey. 2008. Evaluation of potato germplasm for frost tolerance. Indian J Hort 65(3):344-346. (Abstract)<br /> <br /> McCue, K.F., D.R. Rockhold, P.V. Allen, and W.R. Belknap. 2008. Useful promoters and terminators from Solanum bulbocastanum for use in potatoes and other crops. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Morante, M.C., I. Tolin Tordoya and N. Montealegre Villanueva. 2007. Resistance to Phytophthora infestans Mont de Bary of phureja potato (Solanum phureja Juzepczuk et Bukasov) and populations of wild species of potato, La Paz, Bolivia. Pl Genet Resources Newsletter 151:43-48. (Abstract)<br /> <br /> Morris, W.L., H.A. Ross, L.J.M. Ducreux, J.E. Bradshaw, G.J. Bryan, and M.A. Taylor. 2007. Umami compounds are a determinant of the flavor of potato (Solanum tuberosum L.). J Ag Food Chem 55(23):9627-9633. (Abstract)<br /> <br /> Mosley, A.R., S. Yilma, D.C. Hane, S.R. James, K.A. Rykbost, C.C. Shock, S.L. Love, D.L. Corsini, J.J. Pavek, R.E. Thornton, B.A. Charlton, E.P. Eldredge, R.G. Novy, M.J. Pavek, N.R. Knowles, J.L. Whitworth, C.R. Brown, J.C. Stark, and M.I. Vales. 2008. Willamette: A chipping cultivar with high yield and specific gravity, low incidence of hollow heart and brown center, and suitability for fresh-market usage. Am J Potato Res 85:85-92.<br /> <br /> Mugniery, D., O. Plantard, S. Fournet, E. Grenier, B. Caromel, M.C. Kerlan, D. Picard, and D. Ellisseche. 2007. Evaluation of effectiveness and durability of resistance to Globodera pallida from Solanum vernei, S. spegazzinii and S. sparsipilum. Nematol Mediterranea 35(2):143-153. (Abstract)<br /> <br /> Navarre, Roy, Roshani Shakya, Aymeric Goyer, Joanne Holden, Lavanya Reddivari, and Creighton Miller. 2008. Potato phytonutrient analysis and engineering. Phytochemical Society of North America Annual Meeting, Pullman, WA, June 2008. (Abstract) <br /> <br /> Nichol, N.L., K. Zarka, J. Coombs, and D.S. Douches. 2008. CBF1 transgenic potatoes studied under drought conditions. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Novy, R.G., K.B. Kelley and J.L. Whitworth. 2008. Introgression of virus resistances from Solanum etuberosum into cultivated potato and the identification of molecular markers linked to it potato leafroll virus (PLRV) resistance. The 17th Triennial Conf. of the European Assoc. for Potato Research, Brasov, Romania. 7/8/08. Abstracts of Papers and Posters, pp. 70-71. (Abstract) <br /> <br /> Novy, R.G., J.L. Whitworth, J.C. Stark, S.L. Love, D.L. Corsini, J.J. Pavek, M.I. Vales, S.R. James, D.C. Hane, C.C. Shock, B.A. Charlton, C.R. Brown, N.R. Knowles, M.J. Pavek, T.L. Brandt, and N. Olsen. 2008. Premier Russet: A dual-purpose, potato cultivar with significant resistance to low temperature sweetening during long-term storage. Am J Potato Res 85:198-209.<br /> <br /> Pelletier, Y. and D. De Koeyer. 2008. The use of Solanum oplocense as a source of resistance to the Colorado potato beetle, Leptinotarsa decemlineata (Say.). Symposium Insects of Solanaceous crop at the International Congress of Entomology, July 6-12, 2008, Durban, South Africa. <br /> <br /> Pelletier, Y. and D. De Koeyer. 2008. Solanum oplocense as a source of resistance to the Colorado potato beetle and improved processing quality. XXIIIrd Association Latino Americano de la Papa Annual, Mar del Plata, Argentina, Nov. 30th to Dec. 5th. (Poster)<br /> <br /> Peloquin, S.J., L.S. Boiteux, P.W. Simon, and S.H. Jansky. 2008. A Chromosome-specific estimate of transmission of heterozygosity by 2n gametes in potato. J Heredity 99(@):177-181. (Abstract)<br /> <br /> Pennycooke, J.C., H.M. Cheng, S.M. Roberts, Q.F. Yang, S.Y. Rhee, and E.J. Stockinger. 2008. The low temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a genomic environment undergoing gene duplications, deletions, and rearrangements. Pl Mol Bio 67(5):483-497. (Abstract)<br /> <br /> Pino, M.T., J.S. Skinner, Z. Jeknic, P.M. Hayes, A.H. Soeldner, M.F. Thomashow, and T.H.H. Chen. 2008. Ectopic AtCBF1 over-expression enhances freezing tolerance and induces cold acclimation-associated physiological modifications in potato. Pl Cell Environ 31(4):393-406. (Abstract)<br /> <br /> Polkowska-Kowalczyk, L., B. Wielgat and U. Maciejewska. 2007. Changes in the antioxidant status in leaves of Solanum species in response to elicitor from Phytophthora infestans. J Pl Physio 164(10):1268-1277. (Abstract)<br /> <br /> Pompon, J. and Y. Pelletier. 2008. Xylem consumption in Macrosiphum euphorbiae aphid biology. Royal Entomological Society Annual Meeting, Plymouth, UK.<br /> <br /> Pompon, J., D. Quiring, P. Giordanengo, and Y. Pelletier. 2008. Search for new means to control aphid potato pests: resistance mechanism of Solanum chomatophilum to Macrosiphum euphorbiae and Myzus persicae. Annual Meeting of the Entomological Society of Canada, Oct. 19-23, Ottawa, Canada.<br /> <br /> Reddivari, L., J. Vanamala, S. Chintharlapalli, S.H. Safe, and J.C. Miller Jr. 2007. Anthocyanin fraction from potato extracts is cytotoxic to prostate cancer cells through activation of caspase-dependent and caspase-independent pathways. Carcinogenesis 38(10):2227-2235. (Abstract)<br /> <br /> Rockhold, David R., Sophie Chang, Nathan Taylor, Paul V. Allen, Kent F. McCue, and William R. Belknap. 2008. Structure of two Solanum bulbocastanum polyubiquitin genes and expression of their promoters in transgenic potatoes. Am J Potato Res 85:219-226.<br /> <br /> Rosenthal, S. and S.H. Jansky. 2008. Effect of production site and storage on antioxidant levels in specialty potato (Solanum tuberosum L.) tubers. J Sci Food Ag 88:2087-2092.<br /> <br /> Roshani, S. and D.A. Navarre. 2008. LC-MS analysis of solanidane glycoalkaloid diversity among four wild potato species and three cultivars. J Ag Food Chem 56:6949-6958. <br /> <br /> Santa Cruz, J.H., B.J. Christ and K.G. Haynes. 2008. Inheritance of resistance to early blight disease in a diploid hybrid Solanum phureja-S. stenotomum population after one cycle of recurrent selection. American Phytopathological Society Meeting, July 26-30, 2008, Minneapolis, MN. (Abstract)<br /> <br /> Scotti, N., S. Cozzolino and T. Cardi. 2007. Mitochondrial DNA variation in cultivated and wild potato species (Solanum spp.). Genome 50(8):706-713. (Abstract)<br /> <br /> Shakya, Roshani and Duroy A. Navarre. 2008. LC-MS analysis of glycoalkaloid diversity among seven potato genotypes. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Shaterian, J., D.R. Waterer, H. de Jong, and K.K. Tanino. 2008. Methodologies and traits for evaluating the salt tolerance in diploid potato clones. Am J Potato Res 85:93-100.<br /> <br /> Simko, I., J.H. van den Berg, D. Vreugdenhil, and E.E. Ewing. 2008. Mapping loci for chlorosis associated with chlorophyll b deficiency in potato. Euphytica 162(1):99-107. (Abstract)<br /> <br /> Sliwka, J., I. Wasilewicz-Flis, H. Jakuczun, and C. Gebhardt. 2008. Tagging quantitative trait loci for dormancy, tuber shape, regularity of tuber shape, eye depth and flesh color in diploid potato originated from six Solanum species. Pl Breed 127(1):49-55. (Abstract)<br /> <br /> Song, Ye-Su and Andrea Schwarzfischer. 2008. Development of STS markers for selection of extreme resistance (Rysto) to PVY and maternal pedigree analysis of extremely resistant cultivars. Am J Potato Res 85:159-170.<br /> <br /> Sorensen, K.K., H.G. Kirk, K. Olsson, R. Labouriau, and J. Christiansen. 2008. A major QTL and an SSR marker associated with glycoalkaloid content in potato tubers from Solanum tuberosum x S. sparsipilum located on chromosome I. Theor Appl Genet 117(1):1-9. (Abstract)<br /> <br /> Suttle, Jeff. 2008. Symposium Introduction: Enhancing the nutritional value of potato tubers. Am J Potato Res 85:266.<br /> <br /> Tabaldi, L.A., F.T. Nicoloso, G.Y. Castro, D. Cargnelutti, J.F. Goncalves, R. Rauber, E.C. Skrebsky, M.R.C. Schetinger, V.M. Morsch, and D.A. Bisognin. 2007. Physiological and oxidative stress responses of four potato clones to aluminum in nutrient solution. Brazilian J Pl Physio 19(3):211-222. (Abstract)<br /> <br /> Thieme, R., E. Rakosy-Tican, T. Gavrilenko, O. Antonova, J. Schubert, M. Nachtigall, U. Heimbach, and T. Thieme. 2008. Novel somatic hybrids (Solanum tuberosum L. + Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theor Appl Genet 116(5):691-700. (Abstract)<br /> <br /> Thompson, A.L., B.L. Farnsworth, N.C. Gudmestad, G.A. Secor, D.A. Preston, J.R. Sowokinos, M. Glynn, and H. Hatterman-Valenti. 2008. Dakota Diamond: An exceptionally high yielding, cold chipping potato cultivar with long-term storage potential. Am J Potato Res 85:171-182.<br /> <br /> Thompson, Asunta L., Stephen L. Love, Joseph R. Sowokinos, Michael K. Thornton, and Clinton C. Shock. 2008. Review of the sugar end disorder in potato (Solanum tuberosum, L.). Am J Potato Res 85:375-386.<br /> <br /> Tome, L.G.O., L.C. Davide, C.A.B.P. Pinto, A.A. Alves, and C.C. Salgado. 2007. Pollen viability and meiotic analysis of Solanum commersonii commersonii Dun., Solanum commersonii malmeanum Bitt. and Solanum tuberosum L. Crop Breed Appl Biotech 7(4):387-393. (Abstract)<br /> <br /> Trofimov, R.N., S.N. Petukhov, N.N., Morozova, and S.I. Liorek. 2007. Using diploid species and dihaploids to intensify potato breeding. Kartofel i Ovoshchi no. 6. (Abstract)<br /> <br /> Vales, M.I., R.J. Ottoman, J.A. Ortega, O. Riera-Lizarazu, and S. Yima. 2008. Breeding for resistance to PVY in tetraploid potatoes using marker assisted selection. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Valkonen, J.P.T., K. Wiegmann, J.H. Hamalainen, W. Marczewski, and K.N. Watanabe. 2008. Evidence for utility of the same PCR-based markers for selection of extreme resistance to Potato virus Y controlled by Rysto of Solanum stoloniferum derived from different sources. Ann Appl Bio 152(1):121-130. (Abstract)<br /> <br /> Valverde, R. 2007. Genetic mapping and detection of QTLs in Solanum species. Agro Costarricense 31(2):31-47. (Abstract)<br /> <br /> Voronkova, E.V., V.M. Lisovskaja, and A.P. Yermishin. 2007. Diploid hybrids between allotetraploid wild potato species Solanum acaule Bitt., S. stoloniferum Schltdl. and dihaploids of S. tuberosum L. Russian J Genet 43(8):882-889. (Abstract) <br /> <br /> Vossen, E. van der, R. Hutten, V. Vleeshouwers, E. Jacobsen, and R. Visser. 2007. Development of a sustainable Phytophthora resistance strategy in potato. Gewasbescherming 38(5):257-264. (Abstract)<br /> <br /> Vysniauskiene, R., Z. Jancys and R. Spalinskas. 2006. Impact of low temperature on superoxide dismutase (SOD) activity of frost resistant hybrids of potatoes. Sodininkyste ir Darzininkyste 25(4):331-338. (Abstract)<br /> <br /> Wang, MiQia, S. Allefs, R.G. van den Berg, V.G.A.A. Vleeshouwers, E.A.G. van der Vossen, and B. Vosman. 2008. Allele mining in Solanum: conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum. Theor Appl Genet 116(7):933-943. (Abstract)<br /> <br /> Watkinson, J.I., L. Hendricks, A.A. Sioson, L.S. Heath, H.J. Bohnert, and R. Grene. 2008. Tuber development phenotypes in adapted and acclimated, drought-stressed Solanum tuberosum ssp. andigena have distinct expression profiles of genes associated with carbon metabolism. Pl Physio Biochem 46(1):34-45. (Abstract)<br /> <br /> Whitworth, J.L., R.G. Novy, D.G. Hall, J.M. Crosslin, and C.R. Brown. 2008. Characterization of broad spectrum Potato virus Y resistance in a Solanum tuberosum ssp. andigena-derived population and select breeding clones using molecular markers, grafting, and field inoculations. Am J Potato Res (In press)<br /> <br /> Yencho, G. Craig, Per H. McCord, Kathleen G. Haynes, and S.B. Rikki Sterrett. 2008. Internal Heat Necrosis of Potato  A Review. Am J Potato Res 85:69-76.<br /> <br /> Zarka, D., D. Douches and W. Kirk. 2008. Progress in mapping and cloning of a late blight R-gene from S. microdontum. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Zebarth, B.J., T.R. Tarn, H. de Jong, and A. Murphy. 2008. Nitrogen use efficiency characteristics of andigena and diploid potato selections. Am J Potato Res 85:210-218.<br /> <br /> Zhang, Y., C.S. Jung and W.S. de Jong. 2008. Loci that influence anthocyanins pigmentation of potato tuber flesh. 92nd Annual Meeting of The PAA, Buffalo, NY, August 10-14, 2008. (Abstract)<br /> <br /> Theses Reporting Research with NRSP-6 Stocks<br /> <br /> --------------------------------------------<br /> <br /> Butterbach, P. 2007. Molecular evolution of the disease resistance gene Rx in Solanum. Thesis, Wageningen University, Wageningen, Netherlands. 141 pp.<br /> <br /> Cruz, Aida Juarez. 2008. Defense reactions of wild Solanum ssp. against the attack of Phytophthora infestans (Mont.) de Bary, in Toluca Valley, Mexico State. MS Thesis, Universidad Autonoma Chapingo, Mexico. <br /> <br /> Marcial, Noris Dalia Calderon. 2008. Some defensive reactions in different Solanum ssp. for races of Phytophthora infestans (Mont.) de Bary. MS Thesis, Universidad Autonoma Chapingo, Mexico.<br /> <br /> Santa Cruz Hidalgo, Jose H. 2008. Inheritance and marker-assisted selection for resistance to early blight disease in a diploid hybrid Solanum phureja-S. stenotomum population after one cycle of recurrent selection. MS Thesis, Dept. Plant Pathology, Penn State University.<br /> <br />

Impact Statements

1. Beyond providing stocks, NRSP-6 staff members are involved in discovering and developing associated germplasm tools and information. Among these are self compatibility, gibberellin, and 2n gamete mutants; cut-stem pollination, hormone pre-treatment of seeds for better germination, haploid- extracting pollinators, and 2n gamete breeding technique. Yukon Gold, one of the most popular and name-recognized tablestock cultivars, has S. phureja 195198, an exotic cultivated species from NRSP-6 as a grandparent, and was bred using the 2n gamete technique.
2. Evaluation for a wide variety of useful traits has also been designed, contracted and documented by staff. Such work is the foundation for deploying exotic genes in new cultivars. One recent example is the release of cultivar PA99N82-4 bred with the Mexican wild species S. bulbocastanum from NRSP-6. It has high resistance to nematodes that can only be controlled by fumigation at an estimated cost of $20M per year, not counting the cost in risks to human and environmental health posed by use of toxic chemicals.
3. The genebank goal is maximum diversity. But because funds for collecting, preserving, distributing and evaluating are limited, reaching that goal depends on maximizing efficiency through quality control and technology R&D. Thus, we collaborate with other world genebanks to study the partitioning and vulnerability of diversity in our collections. Examples of impact of this area are the intergenebank potato database, identification of more diversity-intense sites for future collecting, and confirming that the rare alleles within some populations within certain species are not explained by introgression of alleles common in another sympatric species.
4. One way the overall impact of these contributions can be measured is by the occurrence of NRSP-6 germplasm in the pedigrees of new, improved potato cultivars. About 70% of all potatoes grown in the United States have germplasm from the genebank in their pedigrees. Both cultivar releases published in the American Journal of Potato Research in 2008, Premier Russet and Dakota Diamond, have exotic species from NRSP-6 in their pedigrees. The great-grandmother of the latter is S. chacoense 472812, a wild potato species originally collected in Argentina.
5. Another gauge of impact is in the numerous publications in 2008 providing information that pushes potato science forward. Section 6 lists 37 papers, 85 abstracts, and 4 theses which report the results of studies associated with NRSP-6 Solanum stocks this year

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Report Information

Participants

List of participants in the 2010 TAC meeting is provided in "minutes", p. 2-8 of the attached comprehensive document.

A list of all current NRSP6 TAC members is provided on the project website: http://www.ars-grin.gov/nr6.

Brief Summary of Minutes

THE DOCUMENT ATTACHED INCLUDES TAC10 MEETING MINUTES AND ALL ASSOCIATED REPORTS
Additional information is available on the project website:
http://www.ars-grin.gov/nr6

Accomplishments

The Accomplishments of this project are contained in the Annual Report starting on p. 10 of the attached complete document recording the TAC10 meeting. Additional details are available at the project website: http://www.ars-grin.gov/nr6.<br /> <br />

Publications

Publications are listed on pages 15-22 of the attached complete document.

Impact Statements

1. An impact statement specific for the previous year is given on page 14 of the attached document. A more comprehensive impact statement is available at the project website: http://www.ars-grin.gov/nr6. Pages 23-55 of the attached document provide the impact of project activities as reported by representatives of the Northcentral, Southern, Northeastern, Western and USDA/ARS representatives at the meeting.

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