Annual/Termination Reports:

[01/18/2005] [07/28/2005]

Report Information

Participants

Mueller,Tom (tmueller@utk.edu); Cobb, Jean (jmcc@vt.edu); Watson, Elizabeth (ewatson@vt.edu); Mersie, Wondi (wmersie@vsu.edu); Dumas, José (jose_dumas@cca.uprm.edu); Massey, Joe (jmassey@pss.mmstate.edu); Mattice, John (jmattice@uark.edu); Senseman, Scott (s-senseman@tamu.edu); Riley, Melissa (mbriley@clemson.edu); Watson, Clarence (cwatson@mafes.msstate.edu);

Brief Summary of Minutes

Minutes for S1011 Meeting
November 18-19, 2004


Attendees: Tom Mueller, Jean Cobb, Elizabeth Watson, Wondi Mersie, José Dumas, Joe Massey, John Mattice, Clarence Watson, Melissa Riley

Tom Mueller is serving as the chair of the group and leader of discussions. Melissa Riley served as the recorder of minutes.

Initial discussions were on editing the paper to be submitted to Journal of Agriculture and Food Chemistry. The draft of the paper was projected onto screen so all members could make their suggestions. Once agreement was obtained the changes were made. Some final changes were made after the meeting such as changes in the numbering of references, changes in the format of tables, and checking that all trademark symbols were incorporated. (Paper was submitted on January 10, 2005)

Clarence Watson, administrative advisor for the group discussed some of the changes in the reporting of the work associated with the Multi-State projects. Information in now incorporated on the NIMMS site which will serve as the repository of information, including the minutes. Last years minutes need to be sent to Clarence so that he can put them into the NIMMS system. Current minutes also need to be sent.

Discussion was made on the use of the old S271 server and it was decided that this server has outlived its usefulness and will be discontinued.

A discussion was conducted into the possibility of looking for outside additional funding for the current project. Currently only one member of the project is receiving any money for the supplies needed to conduct this project. Due to the lack of money for the actual conduct of the research for this project, the group decided that we needed to look for possible small grants to fund the research of the group. Potential sources of additional funding for the research to be pursued by the group should be sent to Tom Mueller.

There was a discussion of the possible site for the meeting of the group next year (2005). Virginia Tech offered to host the meeting next year and this site was accepted unanimously by the group. The meeting will be moved to sometime in June or the first of July. Scott Senseman will be going on sabbatical probably mid-July. Jean Cobb will work with on the final date  everyone should send dates that will not work to Jean as soon as possible and she will establish a date for the meeting.

The work to be conducted for the next year with the project was discussed. One of the major problems that we need to address before we can look at the field extraction is the effect of higher temperature on the pesticides that are adsorbed to the disk materials (Empore and Speedisk) that may occur during the shipment of disk during summer conditions. Previous research has been conducted on room temperature and less than room temperature but there is no research reported on higher temperatures. John Mattice agreed to send out the first draft of the protocol for the next year protocol. Various other details of this part of the project will be decided later.

State Reports were the next part of the group meeting:

Mississippi  Joe Massey  looking at runoff on turf, attempting to get better runoff data  EPA wanting more large plots to better represent what happening
Also looking at the effect of mowing heights of grass and the effect on pesticide runoff
Research uses a rainfall simulator to produce 1 inch/hour  testing 2,4,D, chlorpyrifos, flutalonil and getting 20-25% runoff  for analysis injecting 500 ul of water on HPLC column

Virginia Tech  Jean Cobb  the laboratory is primarily a service center with excellent relationships with the vet school, looking at box turtle liver and crab hemolymph for pesticides, running 100 salamander tails for OCs, DDE and DDTs
John Hess will be retiring in July  likely replacement as department head will be more molecular in focus
William & Mary, Univ. of Virginia and Virginia Tech are discussing the possibility of becoming charter universities  possibly the whole experimental service will be dissolved.

Puerto Rico  José Dumas  monitoring interstitial water in Jobos Bay in Salinas. Also working with carbonatic soils where it is important to determine sorption and desorption constants of some commonly used pesticides, also looking at the saline absorption ratios, surveying wells and interstitial water close to solid waste facilities for nutrients and heavy metals.

Texas A&M  Scott Senseman  working with metolachlor and atrazine, also looking at clomazone use in rice and why it causes injury in some cases but not in others, using centrifugation  plant available water and therefore herbicides  use minimal amount of water
Also looking at other compounds  imazapyr, 2,4 D, and paraquat
Looking at the KD values and % total chlorophyll in plant, after 60% chlorophyll loss then plant cannot recover
May be teaching a mode of action/environmental fate course in Brazil next year during a sabbatical leave there

Virginia State University  Wondi Mersie  working with switchgrass  has small seeds and doesnt germinate very well  trying to look at soil types, moisture, planting depth
USAID project  control methods for parthenium in Ethiopia, serious weeds with lots of crops  also taints milk and causes respiratory problems

Arkansas  John Mattice  monitoring water of rivers in Arkansas and Missouri  all of the sampling is in rice production areas  funded by the rice board  trying to prevent future problems, looking for 10 compounds every 2 weeks from May through August, dont report any detections of < 2 ppb

South Carolina  Melissa Riley  also primarily a service laboratory  measuring ergosterol as a bioindicator of fungi in plant samples, also looking at levels of carotene and lycopene in tomato and their response to different light conditions, running some samples from golf courses for herbicides and looking at fludioxinil levels on peach fruit at the end of the processing lines (used for the control of brown rot during shipment/storage after harvest), looking a stink bugs in cotton and the possible compounds detected by an electronic nose  to develop a cheaper more selective sensor for the detection of stink bug damage prior to visible symptoms, measuring glucosinolates in Brassica species and their possible use in weed suppression and disease suppression.

Tennessee  Tom Mueller  using 2 mm HPLC column  less volume infected
PhD students investigating a Roundup resistant plant  looking at shikimate pathway
Hybrid Bermuda grass  looking at carbohydrate
Poison ivy  research into control, best material to investigate  working with research dermatologist - looking at how long it lasts in mulch
It was suggested he contact Antoine Boudoine at Virginia Tech on biological control options.

Various action items and person responsible for action

Task Person Target Date
Prepare draft protocol for extractor Mattice 12/20/04
Submit meeting grant to Syngenta Mueller 1/05
Draft protocol for heating study Mattice 12/4/04
Finish paper and submit Riley 1/05
Solicit JT Baker for donation of disks Cobb 1/05
Bring letter of collaboration to June meeting Everyone 6/05
Draft boiler plate letter Mueller 3/05
Minutes Riley 12/04

Possible suggestions for reviewers for paper
Jason Krautz  USDA-ARS Stoneville
Don Wauchope  USDA-ARS Tifton


Addresses of Members  November 2004

Tom Mueller tmueller@utk.edu Phone: 865-974-8805
252 Ellington Bldg. FAX: 865-974-5365
2431 Joe Johnson Drive
Knoxville, TN 37996-4561

Jean Cobb jmcc@vt.edu Phone: 540-231-6443
Pesticide Residue Laboratory FAX: 540-231-9070
352 Litton Reaves Hall MC 0309
Virginia Tech
Blacksvurg, VA 24061

Elizabeth Watson ewatson@vt.edu Phone: 540-231-6443
Pesticide Residue Laboratory FAX: 540-231-9070
352 Litton Reaves Hall MC 0309
Virginia Tech
Blacksvurg, VA 24061

Wondi Mersie wmersie@vsu.edu Phone: 804-524-5819
P. O. Box 9061 FAX: ????
Petersburg, VA 23806

Joe Massey jmassey@pss.mmstate.edu Phone : 662-325-4725
117 Dorman Hall FAX: ?????
MississippiState University
Mississippi State, MS 39762

John Mattice jmattice@uark.edu Phone: 479-575-6791
Altheimer Lab FAX: 479-575-3957
1366 Altheimer Drive
Fayetteville, AR 72704

José Dumas jose_dumas@cca.uprm.edu Phone:787-767-9705 x2131
University of Puerto Rico
Agricultural Experiment Station
Botanical Garden  South
1193 Guayacan Street
San Juan, PR 00926-1118

Melissa Riley mbriley@clemson.edu Phone: 864-656-0580
120 Long Hall FAX: 864-656-0274
Entomology, Soils, and Plant Sciences
Clemson University
Clemson, SC 29634-0315

Clarence E. Watson cwatson@mafes.msstate.edu Phone:662-325-0868
Associate Director FAX: 662-325-3001
Mississippi Agricultural and Forestry Experiment Station
210 Bost Center
Box 9740
Mississippi State, MS 39762

Scott A. Senseman s-senseman@tamu.edu Phone:979-845-5375
Associate Professor Fax: 979-845-0456
Texas A&M University
Texas Agricultural Experiment Station
2474 TAMU
College Station, TX 77843-2474

Accomplishments

Publications

Impact Statements

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Report Information

Participants

Cobb, Jean (jmcc@vt.edu) Virgina Tech

Watson, Elizabeth (ewatson@vt.edu) Virgina Tech

Dumas, Jose (jose_dumas@cca.uprm.edu) University of Puerto Rico

Mueller, Tom (tmueller@utk.edu) University of Tennesse-Knoxvile

Mattice, john (jmattice@uark.edu) University Of Arkansas

Riley, Lissa (mbriley@CLEMSON.EDU) Clemson University

Hess, John (jlhess@vt.edu) Virginia Tech

Watson, Clarence (cwatson@mafes.msstate.edu) Mississippi State University

Brief Summary of Minutes

Minutes for S1011 Meeting

Virginia Tech, June 9-10, 2005



Tom Mueller opened the meeting up with a discussion of the agenda. Need to change the agenda due to several people needed to leave early tomorrow. Need to spend a majority of the time looking at the data and work on the development of tables, followed by the state reports, and a discussion of when/where to meet next year. Decided to change the agenda and move the state reports to 10:45 am.



Lissa Riley presented copies of the research paper that was recently accepted by Journal of Agriculture and Food Chemistry concerning previous work conducted by the group.



Clarence Watson, Administrative Advisor, discussed the requirements that he needs to have fulfilled. The minutes need to be sent to him right after the meeting. Minutes will go on the NIMSS website. There was a move this year to eliminate all multi-state research funds in the federal budget, which would have seen fewer multi-state projects but now looks unlikely to be passed. For next year will probably be going back to formula funding.



John Hess, previous department head of the Department of Biochemistry, served as the official representative on the S1011 project at Virginia Tech after Roddy Young retired welcomed the group to Virginia Tech. In January this year he became the interim Dean which he will probably be doing for another year. He stated that when he became involved in the project. He was impressed with the group and could see that something was actually getting done within the group.



Virginia Tech is now a covered (not public) university  they are trying this to get more independence concerning purchasing and hiring and less restriction on tuition control. They have to assess the contribution of the university to students. The personnel are still a part of the state pension system. The change has resulted in more autonomy in raising tuition. In questioning members of the group, most universities among the group had seen tuition up somewhere in the 5-10% range. Seeing more agriculture related families having problems getting their children into the universities. Virginia Tech has an agriculture undecided major that students can come into. College of Agriculture at Virginia Tech has around 2000 students. The Dean of Undergraduate School would like to increase the number to 2200.




Discussion concerning development of the paper/data related to current year's research:



The order of the authors was decided Jean Cobb will the first author, John Mattice - second author, Scott Senseman - third author. Joe Massey will not be an author because he was unable to conduct the test this year. Tom Mueller will be the last author.



Possible Title: Elevated temperatures and extended time intervals affect pesticide recovery from water and solid phase extraction disks in a multi-laboratory study simulating shipping conditions of water



Various technical aspects of the paper were discussed.



State reports from members of the group were presented:



John Mattice - Arkansas

Char is being tested in vegetative filter strips to see if it will help remove pesticides from runoff water. The chars are 600 to 8000 more absorptive than soil toward diuron.



We are using aluminum trays that are designed to allow user to collect leachate water and surface runoff water. Burned Bermuda grass was used as the char - char decreased the amount of diuron and chlorthalonil in runoff water even though it only has a few minutes of contact with the char. Chlorthalonil was absorbed approximately 3 fold faster than diuron. When char was used there was more runoff water and less leachate. Not sure why there is this difference in the amount of runoff/lechate. The char may plug pores in the soil decreasing leaching.



Comparison of using DVB disks instead of C18 disks. DVB shipped with isopropanol to retard microbial growth. The DVB disks can be flushed with methanol and water before going to the field. You don't have to leave the small film of methanol on disks prior to adding the water samples through the disks which is convenient.



A test of the field extraction unit using the DVB speedisk showed it worked well. Recovery of cyhalofop-butyl was enhanced by immediately transferring it to the disk instead of leaving it in water. There were significant losses of cyhalofop-butyl from water after one day.



Monitoring pesticide runoff associated with rice production.

Also conducting some work with antibiotics from chicken litter - used cyano Speedisks and Oasis cartridges - no real way to predict what will work on which disks, found some bacitracin and monencin in litter.



Jose Dumas - University of Puerto Rico

Project T89 - Studying volatile compounds that are produced by sweet potatoes during cooking



Project z177 - Looking for heavy metals and soil absorption ratio - particularly lead and copper



Project zts27 - Looking at pesticide absorption in carbonatic soils



Tom Mueller - University of Tennessee

Several projects related to pesticide fate in the environment and weed resistance to glyphosate



Jean Cobb - Virginia Tech

Facility is mainly a service center - recently had a sophomore undergraduate research student who wanted to get some experience in pesticide analysis - used Quencher's method for the extraction of coumaphos and fluvalinate in honey, working with Rich Fell who has 10 sites in state involving the growers.



Fullbright scholar - looking at organochlorine pesticides and PCB's
Investigating turtle livers from turtles with abcesses.



Sea Duck liners - PCB's - 48 samples looking at PCB's and other compounds.



Lissa Riley - Clemson University

Majority of the work being conducted involved the use of fatty acids for the identification of fungal plant pathogens.



Second project - looking at the levels of fludioxinil on peaches after they have gone through a processing line - fludioxinil is being used for the control of brown rot during shipping.



Doing a lot of analysis related to plant products such as phenolics in fig leaves, essential oil analysis in geranium, and fungicides in peach roots following the injection of fungicide into the trunk.



Business Meeting:

Field extraction apparatus - everyone in the group may not want to build this. Discussed the possibility of not meeting next year, and agreed that this was probably the best thing to do.



Scott Senseman will be out of the country over the next year.



SDC-316 is a new possible project that is looking at chemical sterols .



Meet summer 2007, possibly in Puerto Rico, to do the termination report, field extraction set-up (pesticide analysis of different compounds).



Don't see a common enough theme to have a continuation of a project for 5 years from now -Need to do something else new and different if we develop a new project after the current project.



Adjourned.

Accomplishments

Publications

Riley, Melissa B., Jose A. Dumas, Edward E. Gbur, Joseph H. Massey, John D. Mattice, Wondi Mersie, Thomas C. Mueller, Thomas Potter, Scott A. Senseman, and Elizabeth Watson. 2005. Pesticide Extraction Efficiency of Two Solid Phase Disk Types after Shipping. J. Agric. Food Chem. 53(13):5079-5083.

Impact Statements

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